CN106344775A - Traditional Chinese medicine preparation for preventing and treating black mouth diseases and red spots of sea urchins and preparation method thereof - Google Patents

Traditional Chinese medicine preparation for preventing and treating black mouth diseases and red spots of sea urchins and preparation method thereof Download PDF

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CN106344775A
CN106344775A CN201610874881.9A CN201610874881A CN106344775A CN 106344775 A CN106344775 A CN 106344775A CN 201610874881 A CN201610874881 A CN 201610874881A CN 106344775 A CN106344775 A CN 106344775A
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chinese medicine
black mouth
radix glycyrrhizae
radix isatidis
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王培亮
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Weihai Hongrun Ocean Technology Co Ltd
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Weihai Hongrun Ocean Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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    • A61K36/185Magnoliopsida (dicotyledons)
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    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/31Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A61K36/315Isatis, e.g. Dyer's woad
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484Glycyrrhiza (licorice)
    • AHUMAN NECESSITIES
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    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/896Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8962Allium, e.g. garden onion, leek, garlic or chives
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K2236/30Extraction of the material
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    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30Extraction of the material
    • A61K2236/39Complex extraction schemes, e.g. fractionation or repeated extraction steps

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Abstract

The invention provides a traditional Chinese medicine preparation for preventing and treating black mouth diseases and red spots of sea urchins and a preparation method thereof, which solve the technical problem of low survival rate of existing sea urchins due to the black mouth diseases and the red spots in the process of industrial aquaculture. The traditional Chinese medicine preparation is prepared from the following crude drugs in parts by weight: 30 to 70 parts of herba portulacae, 10 to 30 parts of garlic bulb, 2 to 20 parts of radix isatidis and 2 to 20 parts of liquorice root; the crude drugs are weighed and crushed, water is added to decoct, and then the crude drugs are mixed, mashed, filtered and stirred, so that the traditional Chinese medicine preparation for preventing and treating the black mouth diseases and the red spots of the sea urchins is obtained, and the traditional Chinese medicine preparation can be widely applied to preventing and treating common diseases such as the black mouth diseases, the red spots and the like in the industrial aquaculture of the sea urchins.

Description

A kind of preventing and treating black mouth disease of Hemicentrotus seu Strongylocentrotuss, Chinese medicine preparation of red spot disease and preparation method thereof
Technical field
The present invention relates to a kind of preventing and treating black mouth disease of aquaculture, Chinese medicine preparation of red spot disease and preparation method thereof, especially relate to And a kind of preventing and treating black mouth disease of Hemicentrotus seu Strongylocentrotuss, the Chinese medicine preparation of red spot disease and preparation method thereof, belong to fishery practical technique field.
Background technology
Hemicentrotus seu Strongylocentrotuss are one of economic breed varieties of northern China coastal area, have been enter into the large-scale production stage in the last few years And obtain good benefit.However, in recent years due to factors such as marine pollutions, in the industrialized culture of Hemicentrotus seu Strongylocentrotuss produces, frequently Occur in that the common disease such as " black mouth ", " erythema " leads to Hemicentrotus seu Strongylocentrotuss dead, cause larger loss to Hemicentrotus seu Strongylocentrotuss industrialized culture; Wherein " black mouth disease " is presently the most disease popular and that impact is the most serious.So-called " black mouth disease " is that disease Hemicentrotus seu Strongylocentrotuss membrana perisomialis becomes Black, can not ingest when sb.'s illness took a turn for the worse, adhere to, and Spina jujubae is dead after gradually coming off.This sick initial stage no any exception, but careful sight Examine and can find that Hemicentrotus seu Strongylocentrotuss ingestion ability gradually weakens.Its mortality rate is very high, and economic loss is extremely serious.So how effectively to prevent Industrialized culture Hemicentrotus seu Strongylocentrotuss " black mouth disease " it is particularly important.
Chinese herbal medicine belongs to natural drug, in application and the research of China, has accumulated rich experience.Using Chinese herbal medicine The common disease such as preventing and treating industrialized culture Hemicentrotus seu Strongylocentrotuss " black mouth ", " erythema " is increasingly subject to attention and the favor of people, increasingly wider General acceptance, is that the mechanism of action unique with it, model of action and advantage are inseparable, puts it briefly, and herbal control disease has There are Accommodation, strengthening vital QI to eliminate pathogenic factors, have no drug resistance and the other medicines such as nuisanceless are incomparable and advantage, effect is good, And there is not the residue problem of antibiotic medicine, the aquaculture products of China are exported highly beneficial.In addition, Chinese herbal medicine is also There are low price, wide material sources, the advantages of easy to use, be one of developing direction of fishery medication from now on.
Content of the invention
The invention aims to solving the deficiency of above-mentioned technology, provide a kind of preventing and treating Hemicentrotus seu Strongylocentrotuss black mouth disease, red spot disease Chinese medicine preparation and preparation method thereof.
For this reason, the present invention provides a kind of preventing and treating black mouth disease of Hemicentrotus seu Strongylocentrotuss, the Chinese medicine preparation of red spot disease, by the raw material of following weight portion Medicine is prepared from: 30~70 parts of Herba Portulacae, 10~30 parts of Bulbus Allii, 2~20 parts of Radix Isatidis, 2~20 parts of Radix Glycyrrhizae.
Preferably, a kind of above-mentioned black mouth disease of preventing and treating Hemicentrotus seu Strongylocentrotuss, the Chinese medicine preparation of red spot disease, by the crude drug of following weight portion It is prepared from: 55 parts of Herba Portulacae, 25 parts of Bulbus Allii, 5 parts of Radix Isatidis, 15 parts of Radix Glycyrrhizae.
Preferably, a kind of above-mentioned black mouth disease of preventing and treating Hemicentrotus seu Strongylocentrotuss, the dosage form of the Chinese medicine preparation of red spot disease are decoction.
A kind of above-mentioned black mouth disease of preventing and treating Hemicentrotus seu Strongylocentrotuss, the preparation method of the Chinese medicine preparation of red spot disease, its preparation method is as follows:
(1) crude drug Herba Portulacae, Bulbus Allii, Radix Isatidis, Radix Glycyrrhizae weight portion are pressed, after weighing respectively, clean, pulverizing, standby;
(2) the standby Radix Isatidis of step (1), Radix Glycyrrhizae are added water to cook, first decocts every tank adds water as Radix Isatidis and Radix Glycyrrhizae weight 4~12 times of amount part total amount, keep 2~3 hours after ebuillition of heated, and extracting solution filters through 200 mesh filters and enters settling tank; Second decocts the water heating extraction adding Radix Isatidis and 4~12 times of Radix Glycyrrhizae weight portion total amount, protects boiling 1.5~2 hours, and extracting solution passes through 200 mesh filters filter and enter settling tank, obtain Radix Isatidis and Radix Glycyrrhizae extract;
(3) Radix Isatidis in step (2) settling tank and Radix Glycyrrhizae extract are staticly settled 6~18 hours, extract supernatant Filter through 200 mesh filters, obtain Radix Isatidis and Radix Glycyrrhizae filtrate is standby, precipitate presses offal treatment;
(4) the standby Herba Portulacae of step (1), Bulbus Allii mixing are smashed to pieces, be positioned over ventilation and stand 1.5~2 hours, period Stirring 3~5 times, obtains Herba Portulacae and Bulbus Allii smashs mixture to pieces;
(5) will be water-soluble to the Herba Portulacae of step (4) and Bulbus Allii smashs mixture to pieces with volumetric concentration is 50%~75% ethanol Liquid, according to 1:1 weight than mix and blend, after standing, obtain the mixed liquor of Herba Portulacae and Bulbus Allii;
(6) Herba Portulacae obtaining step (5) and the mixed liquor of Bulbus Allii filter through 200 mesh filters, the filtration obtaining Liquid is added in step (3) Radix Isatidis and Radix Glycyrrhizae filtrate, blended stirring, obtains preventing and treating the black mouth disease of Hemicentrotus seu Strongylocentrotuss, in red spot disease Medicine preparation.
The invention has the beneficial effects as follows: the present invention provide a kind of preventing and treating the black mouth disease of Hemicentrotus seu Strongylocentrotuss, the Chinese medicine preparation of red spot disease and its Preparation method, prevention industrialized culture Hemicentrotus seu Strongylocentrotuss black mouth disease, red spot disease reasonably combined using traditional Chinese herbal medicine;The present invention adopts The Chinese herbal and crude drugs preparationses that four kinds of crude drug are made, it is convenient that crude drug is bought, low cost, and preparation method is simple is it is ensured that water and Hemicentrotus seu Strongylocentrotuss Do not remain poisonous and hazardous chemical agent in vivo.The black mouth disease of industrialized culture Hemicentrotus seu Strongylocentrotuss, red can effectively be prevented and treated using the present invention Pinta, improves Hemicentrotus seu Strongylocentrotuss survival rate, significant to the cultivation of industrialized culture Hemicentrotus seu Strongylocentrotuss, seed breeding.
Specific embodiment
With reference to specific embodiment, the invention will be further described, to help understanding present disclosure.
Select the healthy Hemicentrotus seu Strongylocentrotuss of specification identical 500, point 5 experimental grouies, every group 100, apply following examples 1 respectively What~embodiment 5 was obtained prevents and treats the black mouth disease of Hemicentrotus seu Strongylocentrotuss, the Chinese medicine preparation of red spot disease, with 100 identical rule not applying Chinese medicine preparation The healthy Hemicentrotus seu Strongylocentrotuss of lattice are contrasted, the prevention effect of checking Chinese medicine preparation black mouth disease, red spot disease to Hemicentrotus seu Strongylocentrotuss.This time experiment is in nursery The common culturing pool in workshop, environmental condition is identical with daily Hemicentrotus seu Strongylocentrotuss nursery.
Embodiment 1:
A kind of preventing and treating black mouth disease of Hemicentrotus seu Strongylocentrotuss, the Chinese medicine preparation of red spot disease, its preparation method is as follows:
(1) press raw material medicines in portions by weight, weigh 40 parts of Herba Portulacae, 10 parts of Bulbus Allii, 5 parts of Radix Isatidis, 15 parts of Radix Glycyrrhizae respectively, wash Only after, pulverizing, standby;
(2) Radix Isatidis standby for step (1), Radix Glycyrrhizae are added water to cook, first decocts every tank adds water as Radix Isatidis and Radix Glycyrrhizae weight 4 times of amount part total amount, keep 2 hours after ebuillition of heated, and extracting solution filters through 200 mesh filters and enters settling tank;Second decocts Add the water heating extraction of Radix Isatidis and 6 times of Radix Glycyrrhizae weight portion total amount, protect boiling 1.5 hours, extracting solution is through 200 mesh filter mistakes Filter enters settling tank, obtains Radix Isatidis and Radix Glycyrrhizae extract;
(3) Radix Isatidis in step (2) settling tank and Radix Glycyrrhizae extract are staticly settled 6 hours, extract supernatant and pass through 200 mesh filters filter, and obtain Radix Isatidis and Radix Glycyrrhizae filtrate is standby, and precipitate presses offal treatment;
(4) Herba Portulacae standby for step (1), Bulbus Allii mixing are smashed to pieces, be positioned over ventilation and stand 1.5 hours, period stirs Mix 3~5 times, obtain Herba Portulacae and Bulbus Allii smashs mixture to pieces;
(5) Herba Portulacae of step (4) and Bulbus Allii are smashed to pieces the ethanol water that mixture is 50% with volumetric concentration, according to The weight of 1:1, than mix and blend, after standing, obtains the mixed liquor of Herba Portulacae and Bulbus Allii;
(6) Herba Portulacae obtaining step (5) and the mixed liquor of Bulbus Allii filter through 200 mesh filters, the filtration obtaining Liquid is added in step (3) Radix Isatidis and Radix Glycyrrhizae filtrate, blended stirring, obtains preventing and treating the black mouth disease of Hemicentrotus seu Strongylocentrotuss, in red spot disease Medicine preparation.
Embodiment 2:
A kind of preventing and treating black mouth disease of Hemicentrotus seu Strongylocentrotuss, the Chinese medicine preparation of red spot disease, its preparation method is as follows:
(1) press raw material medicines in portions by weight, weigh 45 parts of Herba Portulacae, 15 parts of Bulbus Allii, 5 parts of Radix Isatidis, 15 parts of Radix Glycyrrhizae respectively, wash Only after, pulverizing, standby;
(2) Radix Isatidis standby for step (1), Radix Glycyrrhizae are added water to cook, first decocts every tank adds water as Radix Isatidis and Radix Glycyrrhizae weight 6 times of amount part total amount, keep 2.5 hours after ebuillition of heated, and extracting solution filters through 200 mesh filters and enters settling tank;Second Decoct the water heating extraction adding Radix Isatidis and 6 times of Radix Glycyrrhizae weight portion total amount, protect boiling 1.5 hours, extracting solution is through 200 mesh filters Filter and enter settling tank, obtain Radix Isatidis and Radix Glycyrrhizae extract;
(3) Radix Isatidis in step (2) settling tank and Radix Glycyrrhizae extract are staticly settled 8 hours, extract supernatant and pass through 200 mesh filters filter, and obtain Radix Isatidis and Radix Glycyrrhizae filtrate is standby, and precipitate presses offal treatment;
(4) Herba Portulacae standby for step (1), Bulbus Allii mixing are smashed to pieces, be positioned over ventilation and stand 1.5 hours, period stirs Mix 3~5 times, obtain Herba Portulacae and Bulbus Allii smashs mixture to pieces;
(5) Herba Portulacae of step (4) and Bulbus Allii are smashed to pieces the ethanol water that mixture is 60% with volumetric concentration, according to The weight of 1:1, than mix and blend, after standing, obtains the mixed liquor of Herba Portulacae and Bulbus Allii;
(6) Herba Portulacae obtaining step (5) and the mixed liquor of Bulbus Allii filter through 200 mesh filters, the filtration obtaining Liquid is added in step (3) Radix Isatidis and Radix Glycyrrhizae filtrate, blended stirring, obtains preventing and treating the black mouth disease of Hemicentrotus seu Strongylocentrotuss, in red spot disease Medicine preparation.
Embodiment 3:
A kind of preventing and treating black mouth disease of Hemicentrotus seu Strongylocentrotuss, the Chinese medicine preparation of red spot disease, its preparation method is as follows:
(1) press raw material medicines in portions by weight, weigh 50 parts of Herba Portulacae, 20 parts of Bulbus Allii, 5 parts of Radix Isatidis, 15 parts of Radix Glycyrrhizae respectively, wash Only after, pulverizing, standby;
(2) the standby Radix Isatidis of step (1), Radix Glycyrrhizae are added water to cook, first decocts every tank adds water as Radix Isatidis and Radix Glycyrrhizae weight 8 times of amount part total amount, keep 2.5 hours after ebuillition of heated, and extracting solution filters through 200 mesh filters and enters settling tank;Second Decoct the water heating extraction adding Radix Isatidis and 8 times of Radix Glycyrrhizae weight portion total amount, protect boiling 2 hours, extracting solution is through 200 mesh filter mistakes Filter enters settling tank, obtains Radix Isatidis and Radix Glycyrrhizae extract;
(3) Radix Isatidis in step (2) settling tank and Radix Glycyrrhizae extract are staticly settled 10 hours, extract supernatant and pass through 200 mesh filters filter, and obtain Radix Isatidis and Radix Glycyrrhizae filtrate is standby, and precipitate presses offal treatment;
(4) the standby Herba Portulacae of step (1), Bulbus Allii mixing are smashed to pieces, be positioned over ventilation and stand 2 hours, period stirring 3 ~5 times, obtain Herba Portulacae and Bulbus Allii smashs mixture to pieces;
(5) Herba Portulacae of step (4) and Bulbus Allii are smashed to pieces the ethanol water that mixture is 70% with volumetric concentration, according to The weight of 1:1, than mix and blend, after standing, obtains the mixed liquor of Herba Portulacae and Bulbus Allii;
(6) Herba Portulacae obtaining step (5) and the mixed liquor of Bulbus Allii filter through 200 mesh filters, the filtration obtaining Liquid is added in step (3) Radix Isatidis and Radix Glycyrrhizae filtrate, blended stirring, obtains preventing and treating the black mouth disease of Hemicentrotus seu Strongylocentrotuss, in red spot disease Medicine preparation.
Embodiment 4:
A kind of preventing and treating black mouth disease of Hemicentrotus seu Strongylocentrotuss, the Chinese medicine preparation of red spot disease, its preparation method is as follows:
(1) press raw material medicines in portions by weight, weigh 55 parts of Herba Portulacae, 25 parts of Bulbus Allii, 5 parts of Radix Isatidis, 15 parts of Radix Glycyrrhizae respectively, wash Only after, pulverizing, standby;
(2) Radix Isatidis standby for step (1), Radix Glycyrrhizae are added water to cook, first decocts every tank adds water as Radix Isatidis and Radix Glycyrrhizae weight 8 times of amount part total amount, keep 2 hours after ebuillition of heated, and extracting solution filters through 200 mesh filters and enters settling tank;Second decocts Add the water heating extraction of Radix Isatidis and 6 times of Radix Glycyrrhizae weight portion total amount, protect boiling 1.5 hours, extracting solution is through 200 mesh filter mistakes Filter enters settling tank, obtains Radix Isatidis and Radix Glycyrrhizae extract;
(3) Radix Isatidis in step (2) settling tank and Radix Glycyrrhizae extract are staticly settled 12 hours, extract supernatant and pass through 200 mesh filters filter, and obtain Radix Isatidis and Radix Glycyrrhizae filtrate is standby, and precipitate presses offal treatment;
(4) Herba Portulacae standby for step (1), Bulbus Allii mixing are smashed to pieces, be positioned over ventilation and stand 2 hours, period stirring 3 ~5 times, obtain Herba Portulacae and Bulbus Allii smashs mixture to pieces;
(5) Herba Portulacae of step (4) and Bulbus Allii are smashed to pieces the ethanol water that mixture is 55% with volumetric concentration, according to The weight of 1:1, than mix and blend, after standing, obtains the mixed liquor of Herba Portulacae and Bulbus Allii;
(6) Herba Portulacae obtaining step (5) and the mixed liquor of Bulbus Allii filter through 200 mesh filters, the filtration obtaining Liquid is added in step (3) Radix Isatidis and Radix Glycyrrhizae filtrate, blended stirring, obtains preventing and treating the black mouth disease of Hemicentrotus seu Strongylocentrotuss, in red spot disease Medicine preparation.
Embodiment 5:
A kind of preventing and treating black mouth disease of Hemicentrotus seu Strongylocentrotuss, the Chinese medicine preparation of red spot disease, its preparation method is as follows:
(1) press raw material medicines in portions by weight, weigh 60 parts of Herba Portulacae, 30 parts of Bulbus Allii, 5 parts of Radix Isatidis, 15 parts of Radix Glycyrrhizae respectively, wash Only after, pulverizing, standby;
(2) the standby Radix Isatidis of step (1), Radix Glycyrrhizae are added water to cook, first decocts every tank adds water as Radix Isatidis and Radix Glycyrrhizae weight 12 times of amount part total amount, keep 3 hours after ebuillition of heated, and extracting solution filters through 200 mesh filters and enters settling tank;Second decocts Add the water heating extraction of Radix Isatidis and 12 times of Radix Glycyrrhizae weight portion total amount, protect boiling 2 hours, extracting solution is through 200 mesh filter mistakes Filter enters settling tank, obtains Radix Isatidis and Radix Glycyrrhizae extract;
(3) Radix Isatidis in step (2) settling tank and Radix Glycyrrhizae extract are staticly settled 14 hours, extract supernatant and pass through 200 mesh filters filter, and obtain Radix Isatidis and Radix Glycyrrhizae filtrate is standby, and precipitate presses offal treatment;
(4) the standby Herba Portulacae of step (1), Bulbus Allii mixing are smashed to pieces, be positioned over ventilation and stand 2 hours, period stirring 3 ~5 times, obtain Herba Portulacae and Bulbus Allii smashs mixture to pieces;
(5) Herba Portulacae of step (4) and Bulbus Allii are smashed to pieces the ethanol water that mixture is 75% with volumetric concentration, according to The weight of 1:1, than mix and blend, after standing, obtains the mixed liquor of Herba Portulacae and Bulbus Allii;
(6) Herba Portulacae obtaining step (5) and the mixed liquor of Bulbus Allii filter through 200 mesh filters, the filtration obtaining Liquid is added in step (3) Radix Isatidis and Radix Glycyrrhizae filtrate, blended stirring, obtains preventing and treating the black mouth disease of Hemicentrotus seu Strongylocentrotuss, in red spot disease Medicine preparation.
What above-described embodiment 1~embodiment 5 prepared prevents and treats the black mouth disease of Hemicentrotus seu Strongylocentrotuss, the Chinese medicine preparation of red spot disease, applies respectively For the first to the 5th experimental group, Chinese medicine preparation and Hemicentrotus seu Strongylocentrotuss breeding water body are splashed by 1:1000 volume ratio, and experimental period is one Week, the black mouth disease of Hemicentrotus seu Strongylocentrotuss, red spot disease to prevent and treat result as shown in table 1 below:
The black mouth disease of table 1 Hemicentrotus seu Strongylocentrotuss, red spot disease prevent and treat result
The Chinese herbal and crude drugs preparationses that can be seen that the application present invention from upper table 1 experimental result can effectively prevent and treat the black mouth disease of Hemicentrotus seu Strongylocentrotuss, red Pinta, reduces the black mouth disease of Hemicentrotus seu Strongylocentrotuss, the M & M of red spot disease, and wherein embodiment 4 effect is best.The present invention is by tradition Medicine reasonable formula, anti-inflammation, enhancing immunity effect extremely notable, be that culture fishery provides a kind of black mouth of Hemicentrotus seu Strongylocentrotuss Disease, the effectively treatment medicine of red spot disease, have instant effect, curative effect really, no drug residue, without any side effects, do not produce drug resistance The feature of property;Its preparation method is simple, and suitable industrial massive cultivates the needs that Hemicentrotus seu Strongylocentrotuss produce.
Only as described above, the only specific embodiment of the present invention, when can not with this limit the present invention enforcement model Enclose, therefore the displacement of its equivalent assemblies, or the equivalent variations made according to scope of patent protection of the present invention and modification, all should still belong to this The category that bright claims are covered.

Claims (4)

1. a kind of preventing and treating the black mouth disease of Hemicentrotus seu Strongylocentrotuss, red spot disease Chinese medicine preparation it is characterised in that: by following weight portion crude drug preparation Form: 30~70 parts of Herba Portulacae, 10~30 parts of Bulbus Allii, 2~20 parts of Radix Isatidis, 2~20 parts of Radix Glycyrrhizae.
2. according to claim 1 a kind of preventing and treating the black mouth disease of Hemicentrotus seu Strongylocentrotuss, red spot disease Chinese medicine preparation it is characterised in that: by such as The crude drug of lower weight portion is prepared from: 55 parts of Herba Portulacae, 25 parts of Bulbus Allii, 5 parts of Radix Isatidis, 15 parts of Radix Glycyrrhizae.
3. according to claim 1 a kind of preventing and treating the black mouth disease of Hemicentrotus seu Strongylocentrotuss, red spot disease Chinese medicine preparation it is characterised in that: described The dosage form of Chinese medicine preparation is decoction.
4. the black mouth disease of a kind of preventing and treating Hemicentrotus seu Strongylocentrotuss according to any one of claim 1 or 2, the preparation of the Chinese medicine preparation of red spot disease Method it is characterised in that: its preparation method is as follows:
(1) described crude drug Herba Portulacae, Bulbus Allii, Radix Isatidis, Radix Glycyrrhizae weight portion are pressed, after weighing respectively, clean, pulverizing, standby;
(2) the standby Radix Isatidis described in step (1), Radix Glycyrrhizae are added water to cook, first decocts every tank adds water as described Radix Isatidis and sweet 4~12 times of careless weight portion total amount, keep 2~3 hours after ebuillition of heated, and extracting solution filters to enter through 200 mesh filters and sinks Shallow lake tank;Second decocts the water heating extraction adding described Radix Isatidis and 4~12 times of Radix Glycyrrhizae weight portion total amount, protects boiling 1.5~2 hours, Extracting solution filters through 200 mesh filters and enters settling tank, obtains Radix Isatidis and Radix Glycyrrhizae extract;
(3) Radix Isatidis in described for step (2) settling tank and Radix Glycyrrhizae extract are staticly settled 6~18 hours, extract supernatant Filter through 200 mesh filters, obtain Radix Isatidis and Radix Glycyrrhizae filtrate is standby, precipitate presses offal treatment;
(4) the standby Herba Portulacae described in step (1), Bulbus Allii mixing are smashed to pieces, be positioned over ventilation and stand 1.5~2 hours, period Stirring 3~5 times, obtains Herba Portulacae and Bulbus Allii smashs mixture to pieces;
(5) will be water-soluble to the Herba Portulacae described in step (4) and Bulbus Allii smashs mixture to pieces with volumetric concentration is 50%~75% ethanol Liquid, according to 1:1 weight than mix and blend, after standing, obtain the mixed liquor of Herba Portulacae and Bulbus Allii;
(6) mixed liquor of the Herba Portulacae obtaining described in step (5) and Bulbus Allii is filtered through 200 mesh filters, the filtration obtaining Liquid is added in the described Radix Isatidis of step (3) and Radix Glycyrrhizae filtrate, blended stirring, obtains preventing and treating the black mouth disease of Hemicentrotus seu Strongylocentrotuss, red spot disease Chinese medicine preparation.
CN201610874881.9A 2016-09-30 2016-09-30 Traditional Chinese medicine preparation for preventing and treating black mouth diseases and red spots of sea urchins and preparation method thereof Pending CN106344775A (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102160874A (en) * 2010-02-20 2011-08-24 大连水产学院 Compound Chinese herbal medicine preparation for treating black mouth disease of sea urchin

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102160874A (en) * 2010-02-20 2011-08-24 大连水产学院 Compound Chinese herbal medicine preparation for treating black mouth disease of sea urchin

Non-Patent Citations (3)

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李登来: "《水产动物疾病学 第二版》", 30 September 2007, 中国农业出版社 *
王进修: "马齿苋在兽医临床上的应用", 《江西畜牧兽医杂质》 *
马现伟: "中草药在兽医临床上的应用", 《吉林畜牧兽医》 *

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