CN106305431A - Anoectochilus roxburghii tissue culture seedling culture medium - Google Patents
Anoectochilus roxburghii tissue culture seedling culture medium Download PDFInfo
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- CN106305431A CN106305431A CN201610985495.7A CN201610985495A CN106305431A CN 106305431 A CN106305431 A CN 106305431A CN 201610985495 A CN201610985495 A CN 201610985495A CN 106305431 A CN106305431 A CN 106305431A
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- China
- Prior art keywords
- salt
- culture medium
- seedling culture
- herba anoectochili
- anoectochili roxburghii
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention discloses an anoectochilus roxburghii tissue culture seedling culture medium which is prepared from the following raw materials: cantaloupe juice, citric acid, O-benzoylsulfonyl imide, apple polyphenol, sylvite, microcosmic salt, nitrogen salt, calcium salt, magnesium salt, iodate, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, ferric salt, vitamin, amino acid, indolebutyric acid, gibberellin, 6 - benzyl gland fat poison, naphthylacetic acid, kinetin, asparagine and water. The anoectochilus roxburghii tissue culture seedling culture medium is reasonable in matching and complete in nutrients, can rapidly promote roots and stems, improve the content of anoectochilus roxburghii polysaccharide, flavonoid compound and alkaloid, shorten the culture period of anoectochilus roxburghii, improve the survival rate of transplanted anoectochilus roxburghii, and lower the production cost.
Description
Technical field
The invention belongs to Herba Anoectochili roxburghii planting technology field, be specifically related to a kind of Herba Anoectochili roxburghii tissue cultured seedling culture medium.
Background technology
Herba Anoectochili roxburghii, also known as Anoectochilus nefiliforme (Nakai) hara, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae), bird Radix Ginseng etc., is famous and precious Valuable Herbal Medicine, for the orchid family Anoectochilus Blume for many years
Raw herbaceous plant, China is mainly distributed on the ground such as Fujian, Taiwan, Zhejiang, Jiangxi, Guangdong and Guangxi.Herba Anoectochili roxburghii with all herbal medicine,
There is the effects such as cooling and internal heat relieving, the solid lung of removing heat from blood, wound of dispelling removing toxic substances, strengthening by means of tonics, among the people, there is extensive medical value, owing to it is controlled
Treat wide, special effect, it is considered as " god's medicine " by among the people, Taiwan is referred to as " king of Chinese medicine "；In recent years, Herba Anoectochili roxburghii is applied to again
The disease such as treatment hypertension, diabetes, hepatitis and tumor, it is medicinal, eat, many fine qualities such as view and admire is increasingly subject to
The favor of people, has wide exploitation prospect.
Gold thread Nelumbo shade plant, light saturation point is low in its natural state, and wild Herba Anoectochili roxburghii is distributed in height above sea level 300-
Between 1200m, the most sporadicly it is distributed in the sylvan life surface layer at the dark and damp places such as relative humidity is high, grass is less, humus is abundant, likes Wen Liang
Humidity and low light environment.Under natural environment, Herba Anoectochili roxburghii growth thermophilic 18-25 DEG C, temperature growth more than 28 DEG C is suppressed, 5 DEG C
Following undergrowth even stops growing, and temperature gradually restores growth after raising；Feeling nice and cool at relative air humidity 85%-92%
The growth of dark and damp weather is preferably.Herba Anoectochili roxburghii is typical shallow root shade plant, and because its seed is little, embryo development is not complete, the most numerous
Growing inferior capabilities, natural production is the most rare, adds and artificially excessively excavates for a long time so that it is wild resource is increasingly exhausted, be on the verge of
Extinction, accordingly, it would be desirable to a large amount of artificial culture is to meet the market demand constantly expanded.
At present, in the breeding system of Herba Anoectochili roxburghii test tube Seedling, root media uses solid medium, solid culture
The application of base, not only adds production cost, and easily causes root injury when seedling taking, reduce transplanting survival rate.
Summary of the invention
The present invention provides a kind of Herba Anoectochili roxburghii tissue cultured seedling culture medium, is to use solid culture to solve existing tissue culture Quito
Base, causes and not only adds production cost, and easily causes root injury when seedling taking, reduces and transplants asking of survival rate
Topic.The Herba Anoectochili roxburghii tissue cultured seedling culture medium collocation of the present invention is reasonable, comprehensive nutrition, it is possible to quickly promotees the raw stem of root, improves Herba Anoectochili roxburghii many
Sugar, flavone compound, the content of alkaloid, shorten Herba Anoectochili roxburghii cultivation cycle, improves Herba Anoectochili roxburghii and transplants survival rate, reduces into product
Cost.
For solve above technical problem, the present invention by the following technical solutions:
A kind of Herba Anoectochili roxburghii tissue cultured seedling culture medium, in units of weight portion, including following raw material: Cantaloupe juice 130-350 part, Fructus Citri Limoniae
Acid 2.3-3.6 part, ortho-sulphobenzimide 0.08-0.12 part, apple polyphenol 1.6-3 part, potassium salt 2.6-4.2 part, microcosmic salt
1.5-3.8 part, nitrogen salt 1.2-2.6 part, calcium salt 0.8-1.3 part, magnesium salt 0.7-1.2 part, iodine salt 0.4-0.6 part, boron salt 0.2-0.4
Part, molybdenum salt 0.2-0.3 part, zinc salt 0.3-0.4 part, manganese salt 0.1-0.3 part, mantoquita 0.1-0.2 part, iron salt 0.1-0.2 part, dimension life
Element 0.12-0.28 part, aminoacid 0.1-0.22 part, indolebutyric acid 0.08-0.14 part, gibberellins 0.08-0.12 part, 6-benzyl gland
Fat purine 0.07-0.09 part, naphthalene acetic acid 0.08-0.14 part, kinetins 0.05-0.09 part, Radix Asparagi door element 0.14-0.26 part, water
2500-3600 part.
Preferably, described potassium salt is potassium chloride；Described microcosmic salt is calcium phosphate；Described nitrogen salt is ammonium hydrogen carbonate；Described calcium salt
For calcium glycine；Described magnesium salt is magnesium glycinate.
Preferably, described iodine salt is potassium iodide.
Preferably, described boron salt is potassium borate.
Preferably, described molybdenum salt is potassium molybdate.
Preferably, described zinc salt is zinc chloride.
Preferably, described manganese salt is Mn-Gly.
Preferably, described mantoquita is cupric glycinate.
Preferably, described iron salt is ferrous sulfate.
Preferably, described vitamin is vitamin C；Described aminoacid is glycine.
The method have the advantages that
The Herba Anoectochili roxburghii tissue cultured seedling culture medium collocation of the present invention is reasonable, comprehensive nutrition, it is possible to quickly promotees the raw stem of root, improves Herba Anoectochili roxburghii many
Sugar, flavone compound, the content of alkaloid, shorten Herba Anoectochili roxburghii cultivation cycle, improves Herba Anoectochili roxburghii and transplants survival rate, reduces into product
Cost.
Detailed description of the invention
For ease of being more fully understood that the present invention, being illustrated by following example, these embodiments belong to the present invention's
Protection domain, but it is not intended to protection scope of the present invention.
In an embodiment, described Herba Anoectochili roxburghii tissue cultured seedling culture medium, in units of weight portion, including following raw material: hami melon
Juice 130-350 part, citric acid 2.3-3.6 part, ortho-sulphobenzimide 0.08-0.12 part, apple polyphenol 1.6-3 part, potassium salt
2.6-4.2 part, microcosmic salt 1.5-3.8 part, nitrogen salt 1.2-2.6 part, calcium salt 0.8-1.3 part, magnesium salt 0.7-1.2 part, iodine salt 0.4-0.6
Part, boron salt 0.2-0.4 part, molybdenum salt 0.2-0.3 part, zinc salt 0.3-0.4 part, manganese salt 0.1-0.3 part, mantoquita 0.1-0.2 part, iron salt
0.1-0.2 part, vitamin 0.12-0.28 part, aminoacid 0.1-0.22 part, indolebutyric acid 0.08-0.14 part, gibberellins 0.08-
0.12 part, 6-benzyl gland fat purine 0.07-0.09 part, naphthalene acetic acid 0.08-0.14 part, kinetins 0.05-0.09 part, Radix Asparagi door element
0.14-0.26 part, water 2500-3600 part.
Described potassium salt is potassium chloride；Described microcosmic salt is calcium phosphate；Described nitrogen salt is ammonium hydrogen carbonate；Described calcium salt is glycine
Calcium；Described magnesium salt is magnesium glycinate；Described iodine salt is potassium iodide；Described boron salt is potassium borate；Described molybdenum salt is potassium molybdate；Described
Zinc salt is zinc chloride；Described manganese salt is Mn-Gly；Described mantoquita is cupric glycinate；Described iron salt is ferrous sulfate.
Described vitamin is vitamin C；Described aminoacid is glycine.
Embodiment 1
A kind of Herba Anoectochili roxburghii tissue cultured seedling culture medium, in units of weight portion, including following raw material: Cantaloupe juice 250 parts, citric acid 3
Part, ortho-sulphobenzimide 0.1 part, apple polyphenol 2.5 parts, potassium salt 3.5 parts, microcosmic salt 2.6 parts, nitrogen salt 2.2 parts, calcium salt 1 part,
Magnesium salt 1 part, iodine salt 0.5 part, boron salt 0.3 part, molybdenum salt 0.25 part, zinc salt 0.35 part, manganese salt 0.2 part, mantoquita 0.15 part, iron salt
0.16 part, vitamin 0.2 part, 0.16 part of aminoacid, indolebutyric acid 0.1 part, gibberellins 0.1 part, 6-benzyl gland fat purine 0.08 part,
Naphthalene acetic acid 0.12 part, kinetins 0.08 part, Radix Asparagi door element 02 part, 3000 parts of water.
Described potassium salt is potassium chloride；Described microcosmic salt is calcium phosphate；Described nitrogen salt is ammonium hydrogen carbonate；Described calcium salt is glycine
Calcium；Described magnesium salt is magnesium glycinate；Described iodine salt is potassium iodide；Described boron salt is potassium borate；Described molybdenum salt is potassium molybdate；Described
Zinc salt is zinc chloride；Described manganese salt is Mn-Gly；Described mantoquita is cupric glycinate；Described iron salt is ferrous sulfate.
Described vitamin is vitamin C；Described aminoacid is glycine.
Embodiment 2
A kind of Herba Anoectochili roxburghii tissue cultured seedling culture medium, in units of weight portion, including following raw material: Cantaloupe juice 130 parts, citric acid
2.3 parts, ortho-sulphobenzimide 0.08 part, apple polyphenol 1.6 parts, potassium salt 2.6 parts, microcosmic salt 1.5 parts, nitrogen salt 1.2 parts, calcium salt
0.8 part, magnesium salt 0.7 part, iodine salt 0.4 part, boron salt 0.2 part, molybdenum salt 0.2 part, zinc salt 0.3 part, manganese salt 0.1 part, mantoquita 0.1 part,
0.1 part of iron salt, vitamin 0.12 part, 0.1 part of aminoacid, indolebutyric acid 0.08 part, gibberellins 0.08 part, 6-benzyl gland fat purine
0.07 part, naphthalene acetic acid 0.08 part, kinetins 0.05 part, Radix Asparagi door element 0.14 part, 2500 parts of water.
Described potassium salt is potassium chloride；Described microcosmic salt is calcium phosphate；Described nitrogen salt is ammonium hydrogen carbonate；Described calcium salt is glycine
Calcium；Described magnesium salt is magnesium glycinate；Described iodine salt is potassium iodide；Described boron salt is potassium borate；Described molybdenum salt is potassium molybdate；Described
Zinc salt is zinc chloride；Described manganese salt is Mn-Gly；Described mantoquita is cupric glycinate；Described iron salt is ferrous sulfate.
Described vitamin is vitamin C；Described aminoacid is glycine.
Embodiment 3
A kind of Herba Anoectochili roxburghii tissue cultured seedling culture medium, in units of weight portion, including following raw material: Cantaloupe juice 350 parts, citric acid
3.6 parts, ortho-sulphobenzimide 0.12 part, apple polyphenol 3 parts, potassium salt 4.2 parts, microcosmic salt 3.8 parts, nitrogen salt 2.6 parts, calcium salt
1.3 parts, magnesium salt 1.2 parts, iodine salt 0.6 part, boron salt 0.4 part, molybdenum salt 0.3 part, zinc salt 0.4 part, manganese salt 0.3 part, mantoquita 0.2 part,
0.2 part of iron salt, vitamin 0.28 part, 0.22 part of aminoacid, indolebutyric acid 0.14 part, gibberellins 0.12 part, 6-benzyl gland fat purine
0.09 part, naphthalene acetic acid 0.14 part, kinetins 0.09 part, Radix Asparagi door element 0.26 part, 364000 parts of water.
Described potassium salt is potassium chloride；Described microcosmic salt is calcium phosphate；Described nitrogen salt is ammonium hydrogen carbonate；Described calcium salt is glycine
Calcium；Described magnesium salt is magnesium glycinate；Described iodine salt is potassium iodide；Described boron salt is potassium borate；Described molybdenum salt is potassium molybdate；Described
Zinc salt is zinc chloride；Described manganese salt is Mn-Gly；Described mantoquita is cupric glycinate；Described iron salt is ferrous sulfate.
Described vitamin is vitamin C；Described aminoacid is glycine.
Above content it cannot be assumed that the present invention be embodied as be confined to these explanation, technology belonging to the present invention is led
For the those of ordinary skill in territory, without departing from the inventive concept of the premise, it is also possible to make some simple deduction or replace,
All should be considered as belonging to the scope of patent protection that the present invention is determined by the claims submitted to.
Claims (10)
1. a Herba Anoectochili roxburghii tissue cultured seedling culture medium, it is characterised in that in units of weight portion, including following raw material: Cantaloupe juice
130-350 part, citric acid 2.3-3.6 part, ortho-sulphobenzimide 0.08-0.12 part, apple polyphenol 1.6-3 part, potassium salt
2.6-4.2 part, microcosmic salt 1.5-3.8 part, nitrogen salt 1.2-2.6 part, calcium salt 0.8-1.3 part, magnesium salt 0.7-1.2 part, iodine salt 0.4-0.6
Part, boron salt 0.2-0.4 part, molybdenum salt 0.2-0.3 part, zinc salt 0.3-0.4 part, manganese salt 0.1-0.3 part, mantoquita 0.1-0.2 part, iron salt
0.1-0.2 part, vitamin 0.12-0.28 part, aminoacid 0.1-0.22 part, indolebutyric acid 0.08-0.14 part, gibberellins 0.08-
0.12 part, 6-benzyl gland fat purine 0.07-0.09 part, naphthalene acetic acid 0.08-0.14 part, kinetins 0.05-0.09 part, Radix Asparagi door element
0.14-0.26 part, water 2500-3600 part.
Herba Anoectochili roxburghii tissue cultured seedling culture medium the most according to claim 1, it is characterised in that described potassium salt is potassium chloride；Described phosphorus
Salt is calcium phosphate；Described nitrogen salt is ammonium hydrogen carbonate；Described calcium salt is calcium glycine；Described magnesium salt is magnesium glycinate.
Herba Anoectochili roxburghii tissue cultured seedling culture medium the most according to claim 1, it is characterised in that described iodine salt is potassium iodide.
Herba Anoectochili roxburghii tissue cultured seedling culture medium the most according to claim 1, it is characterised in that described boron salt is potassium borate.
Herba Anoectochili roxburghii tissue cultured seedling culture medium the most according to claim 1, it is characterised in that described molybdenum salt is potassium molybdate.
Herba Anoectochili roxburghii tissue cultured seedling culture medium the most according to claim 1, it is characterised in that described zinc salt is zinc chloride.
Herba Anoectochili roxburghii tissue cultured seedling culture medium the most according to claim 1, it is characterised in that described manganese salt is Mn-Gly.
Herba Anoectochili roxburghii tissue cultured seedling culture medium the most according to claim 1, it is characterised in that described mantoquita is cupric glycinate.
Herba Anoectochili roxburghii tissue cultured seedling culture medium the most according to claim 1, it is characterised in that described iron salt is ferrous sulfate.
Herba Anoectochili roxburghii tissue cultured seedling culture medium the most according to claim 1, it is characterised in that described vitamin is vitamin C；Institute
Stating aminoacid is glycine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610985495.7A CN106305431A (en) | 2016-11-09 | 2016-11-09 | Anoectochilus roxburghii tissue culture seedling culture medium |
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|---|---|---|---|
| CN201610985495.7A CN106305431A (en) | 2016-11-09 | 2016-11-09 | Anoectochilus roxburghii tissue culture seedling culture medium |
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|---|---|---|---|---|
| CN101124890A (en) * | 2006-08-18 | 2008-02-20 | 上海雷允上科技发展有限公司 | Method for cultivating tissue cultured gloden line lotus seedling |
| CN102499074A (en) * | 2011-09-30 | 2012-06-20 | 杭州鼎韵农业开发有限公司 | Tissue culture medium for anoectochilus roxburghii |
| CN103858762A (en) * | 2014-03-17 | 2014-06-18 | 厦门涌泉科技有限公司 | Rapid proliferation method for anoectochilus formosanus tissue culture |
| CN104855285A (en) * | 2015-04-23 | 2015-08-26 | 福建农林大学 | One-time seedling induction method for organic cultivation of polyploid Anoectochilus roxburghii |
| CN105724249A (en) * | 2016-02-22 | 2016-07-06 | 中国药科大学 | Exogenous additive culture medium for promoting differentiation of anoectochilus formosanus protocorm |
| CN106045699A (en) * | 2016-06-06 | 2016-10-26 | 大新县生产力促进中心 | Pueraria lobata culture medium |
-
2016
- 2016-11-09 CN CN201610985495.7A patent/CN106305431A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101124890A (en) * | 2006-08-18 | 2008-02-20 | 上海雷允上科技发展有限公司 | Method for cultivating tissue cultured gloden line lotus seedling |
| CN102499074A (en) * | 2011-09-30 | 2012-06-20 | 杭州鼎韵农业开发有限公司 | Tissue culture medium for anoectochilus roxburghii |
| CN103858762A (en) * | 2014-03-17 | 2014-06-18 | 厦门涌泉科技有限公司 | Rapid proliferation method for anoectochilus formosanus tissue culture |
| CN104855285A (en) * | 2015-04-23 | 2015-08-26 | 福建农林大学 | One-time seedling induction method for organic cultivation of polyploid Anoectochilus roxburghii |
| CN105724249A (en) * | 2016-02-22 | 2016-07-06 | 中国药科大学 | Exogenous additive culture medium for promoting differentiation of anoectochilus formosanus protocorm |
| CN106045699A (en) * | 2016-06-06 | 2016-10-26 | 大新县生产力促进中心 | Pueraria lobata culture medium |
Non-Patent Citations (5)
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| 张洪昌等: "《植物生长调节剂使用手册》", 30 March 2011 * |
| 张铁: "金线莲组织培养研究进展", 《文山学院学报》 * |
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