CN106278534A - A kind of compound microorganism ferments prepares the method for marine organisms organic liquid fertilizer - Google Patents
A kind of compound microorganism ferments prepares the method for marine organisms organic liquid fertilizer Download PDFInfo
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- CN106278534A CN106278534A CN201610580465.8A CN201610580465A CN106278534A CN 106278534 A CN106278534 A CN 106278534A CN 201610580465 A CN201610580465 A CN 201610580465A CN 106278534 A CN106278534 A CN 106278534A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention relates to a kind of method that compound microorganism ferments prepares marine organisms organic liquid fertilizer.First by fresh marine algae plant clean, remove impurity, immersion, defibrination, prepare the seed liquor of strain the most respectively, seed liquor be inoculated into several times in Sargassum bed material, it is achieved staged fermentation.Experiment shows, the composite ratings fermentation technique effect that the present invention uses is significantly better than single culture fermentation and common multiple bacteria compound fermentation, and fermentation efficiency is high, and the time is short, and whole technique is handling by force, green energy conservation, environmental protection.The living bacteria count using marine organisms organic liquid fertilizer prepared by this technology is 1,500,000,000 more than CFU/ml, and pH value is 6.5 7.0, and N, P, K content ratio is 1 2:1:0.8 1.5.
Description
Technical field
The invention belongs to agricultural fertilizer field, be specifically related to prepare marine organisms by compound microorganism ferments technology organic
The method of liquid fertilizer.
Background technology
At present, the production method of marine organisms organic liquid fertilizer mainly has chemical method, Physical and bioanalysis.Chemistry
Method processes alginic cell mainly by acid, alkali and organic solvent, makes cell clear up release endogenous substance, the method operation letter
Single, easily realize, be also the method for the most most alga fertilizer manufacturing enterprises employing.But, chemical reagent is thin to Sargassum
The destructiveness of the active material ingredients of intracellular is quite big, and the organic reagent remained also is potentially hazardous to environment.Thing
The principle of logos is to control the physical environmental condition such as pressure, temperature, utilizes mechanical force to make alginic cell crush, and content discharges.
Though the method technique cleaning, environmental friendliness, the requirement for instrument and equipment is harsh, and change in reaction conditions is violent, cost
Higher, it is difficult to realize large-scale production.Bioanalysis includes enzymic degradation and two kinds of methods of fermentable, and its cardinal principle is broken
Bad alginic cell wall construction macromolecular substances of degrading are the small-molecule substance that plant easily absorbs, the method reaction temperature
With, whole production process safety and environmental protection is pollution-free.
The research utilizing Production by Microorganism Fermentation marine alga fertilizer is relatively fewer, only a few example researchs be select the most micro-
Biological carry out Sargassum raw material is similar to compost fermentation or aerobic fermentation process, there is sweat poor controllability, and fermentation period is long,
The problems such as target product is indefinite, it is difficult to meet the unification of industrialization production requirements, stable, controlled standard, the quality of product
It is difficult to ensure.
Summary of the invention
A kind of method that present invention aim at providing compound microorganism ferments to prepare marine organisms organic liquid fertilizer, logical
Cross composite bacteria fermentation, make all kinds of bacterial strain play synergism, improve the fertilizer efficiency of organic liquid fertilizer.
For achieving the above object, the present invention is by the following technical solutions:
A kind of compound microorganism ferments prepares the method for marine organisms organic liquid fertilizer, it is characterised in that include following step
Rapid:
1) product A is prepared: fresh marine algae plant is clean, remove impurity, after soaking 12-48h, defibrination, proceed to fermentation
In tank, 110-150 DEG C of sterilizing 15-30min, it is cooled to 20-35 DEG C and obtains product A；
2) seed liquor is prepared: activated by bacillus cereus and spread cultivation, preparing bacillus cereus seed liquor X；Will be addicted to sugar
Bacillus cereus activates and spreads cultivation, and prepares addicted to sugar bacillus cereus seed liquor Y；Being activated by bacillus amyloliquefaciens and spread cultivation, preparation solves
Bacillus amyloliquefaciens seed liquor Z；
3) preparing product B: seed liquor X be inoculated in product A, seed liquor X consumption is the 10%-20% of product A quality,
Fermentation temperature is 30-37 DEG C, is 5-8 with the pH value of acid-base modifier regulation reaction, and dissolved oxygen controls at 10-40%, and rotating speed is
100-250r/min, ventilation should maintain 0.02-0.08Mpa at 500-2000L/min, fermentation tank pressure, and fermentation time is 8-
24h, obtains product B；
4) prepare products C: adjust the pH to 4.0～5.5 of product B, and temperature in fermentation tank is cooled to 0 DEG C～5 DEG C, protect
Deposit 2～5 days, then temperature is recovered to 20 DEG C～35 DEG C, obtain products C；
5) preparing product D: seed liquor Y be inoculated in products C, seed liquor Y consumption is the 10%-20% of products C quality,
Fermentation temperature is 30-34 DEG C, is 5-8 with the pH value of acid-base modifier regulation reaction, and dissolved oxygen controls at 10-40%, and rotating speed is
100-250r/min, ventilation should maintain 0.02-0.08Mpa at 500-2000L/min, fermentation tank pressure, and fermentation time is 8-
24h, obtains product D；
6) prepare product E: adjust the pH to 4.0～5.5 of product D, and temperature in fermentation tank is cooled to 0 DEG C～5 DEG C, protect
Deposit 2～7 days, then temperature is recovered to 25 DEG C～35 DEG C, obtain product E；
7) preparing product F: seed liquor Z be inoculated in product E, seed liquor Z consumption is the 10%-20% of product E quality,
Fermentation temperature is 30-34 DEG C, is 5-8 with the pH value of acid-base modifier regulation reaction, and dissolved oxygen controls at 10-40%, and rotating speed is
100-250r/min, ventilation should maintain 0.02-0.08Mpa at 500-2000L/min, fermentation tank pressure, and fermentation time is 8-
24h, obtains product F；
8) prepare product G: adjust the pH to 4.0～5.5 of product F, and temperature in fermentation tank is cooled to 0 DEG C～5 DEG C, protect
Deposit 2～7 days, then temperature is recovered to 25 DEG C～35 DEG C, obtain product G；
9) marine organisms organic liquid fertilizer is prepared: in product G, add ammonium sulfate 10-15 weight portion, potassium sulfate 8-20
Weight portion, ammonium dihydrogen phosphate 5-15 weight portion, carbamide 10-15 part, be stirred to dissolve the marine organisms uniformly obtaining 100 weight portions
Organic liquid fertilizer.
Described step 1) in marine algae plant be the one in Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.), Thallus Laminariae (Thallus Eckloniae), Brown algae, sargassum thunbergii and Alga Sgrgassi Enerves or
Person's arbitrary proportion two or more.
Described step 2) in bacillus cereus activation with the culture medium prescription spread cultivation be: bean cake powder 20g, yeast extract
6g, sodium alginate 1.2g, bovine bile 0.05g, potassium dihydrogen phosphate 0.04g, sodium chloride 0.2g, magnesium sulfate 0.2g, mannitol 9.5g,
Water 1000mL, regulates pH6.8-7.0, and the condition of spreading cultivation is: cultivate 20h for 33 DEG C.
Described step 2) in addicted to sugar bacillus cereus activation with the culture medium prescription spread cultivation be: sweet potato powder 22g, glutamic acid
0.03g, yeast extract 5g, peptone 11g, manganese dioxide 0.02g, potassium dihydrogen phosphate 0.02g, iron chloride 0.005g, magnesium sulfate
0.2g, calcium carbonate 0.1g, water 1000mL, regulate pH7.2-7.5, and the condition of spreading cultivation is: cultivates 22h for 30 DEG C.
Described step 2) in bacillus amyloliquefaciens activation with the culture medium prescription spread cultivation be: starch 20g, bean cake
40g, yeast extract 6g, potassium dihydrogen phosphate 2.0g, sodium acetate 5.0g, manganese sulfate 0.5g, magnesium sulfate 0.5g, calcium carbonate 0.3g, water
1000ml, regulates pH6.8-7.0, and the condition of spreading cultivation is: cultivate 24h for 35 DEG C.
Described step 3) to step 8) in acid-base modifier be citric acid, oxalic acid, acetic acid, dipotassium hydrogen phosphate, carbonic acid
Sodium, one or more in sodium acetate.
Described step 9) in the living bacteria count of marine organisms organic liquid fertilizer be 1,500,000,000 more than CFU/ml, pH value
For 6.5-7.0, N, P, K content ratio is 1-2:1:0.8-1.5.
Described is that its preserving number is CGMCC No12155 addicted to sugar bacillus cereus YIC～Alg3 addicted to sugar bacillus cereus.
Beneficial effects of the present invention:
1. the living bacteria count of the Chemical Mixed Fertilizer of the present invention is high, all at 1,000,000,000 more than CFU/g.
2. the present invention uses composite bacteria to ferment, and all kinds of bacterial strains play synergism, and effect fertilizer efficiency is significantly better than that single
Strain fermentation.
3. the composite bacteria fermentation that the present invention uses is different from simple by different strain seed liquor mixed fermentation, but
Effect according to microorganism is different, according to precedence successively staged fermentation, it is to avoid vying each other between microorganism species is short of money
Anti-, reduce in-fighting, perform to the effect of respective flora maximize, it is ensured that best effect.
4. the strain that the present invention uses all through strict screening, has high survival and reproduction ability and fermentation efficiency, sends out
Ferment time super many 24h, the minimum 6h that only needs, traditional fermentation then takes several days to tens days.Substantially increase production efficiency,
Save energy consumption and cost.
5. the marine organisms organic liquid fertilizer fertilizer content of beary metal using the technique of the present invention to produce is low, indices
Apparently higher than industry standard, crop yield and resistance can be significantly improved.
Accompanying drawing explanation
Fig. 1 is microexamination YIC～the Gram’s staining of Alg3 bacterial strain and assembles agglomerating phenomenon figure.
Fig. 2 is YIC～Alg3 bacterial strain phylogenetic tree schematic diagram based on 16SrDNA sequence construct.
Detailed description of the invention
In conjunction with specific embodiments technical scheme is described in further detail, but not as to the present invention's
Limit.
Embodiment 1
1) product A is prepared: by clean to fresh Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) and Brown algae (mass ratio 1:1), remove impurity, after soaking 24h, defibrination, turn
Enter in fermentation tank, sterilizing 30min at 115 DEG C, it is cooled to 25 DEG C and obtains product A, standby.
2) seed liquor is prepared: activated by bacillus cereus and spread cultivation, preparing bacillus cereus seed liquor X；Will be addicted to sugar
Bacillus cereus activates and spreads cultivation, and prepares addicted to sugar bacillus cereus seed liquor Y；Being activated by bacillus amyloliquefaciens and spread cultivation, preparation solves
Bacillus amyloliquefaciens seed liquor Z；
Described bacillus cereus activation with the culture medium prescription spread cultivation is: bean cake powder 20g, yeast extract 6g, alginic acid
Sodium 1.2g, bovine bile 0.05g, potassium dihydrogen phosphate 0.04g, sodium chloride 0.2g, magnesium sulfate 0.2g, mannitol 9.5g, water 1000mL,
Regulation pH6.8.The condition of spreading cultivation is: cultivate 20h for 33 DEG C.
Described activating addicted to sugar bacillus cereus with the culture medium prescription spread cultivation is: sweet potato powder 22g, glutamic acid 0.03g, yeast
Cream 5g, peptone 11g, manganese dioxide 0.02g, potassium dihydrogen phosphate 0.02g, iron chloride 0.005g, magnesium sulfate 0.2g, calcium carbonate
0.1g, water 1000mL, regulate pH7.2.The condition of spreading cultivation is: cultivate 22h for 30 DEG C.
Described bacillus amyloliquefaciens activation with the culture medium prescription spread cultivation is: starch 20g, bean cake 40g, yeast extract
6g, potassium dihydrogen phosphate 2.0g, sodium acetate 5.0g, manganese sulfate 0.5g, magnesium sulfate 0.5g, calcium carbonate 0.3g, water 1000mL, regulation
pH7.0.The condition of spreading cultivation is: cultivate 24h for 35 DEG C.
3) preparing product B: seed liquor X be inoculated in product A, inoculum concentration is that 10% (described inoculum concentration refers to seed liquor
Account for the mass percent of fermentation medium, lower same), fermentation temperature is 33 DEG C, regulates the pH value of reaction with citric acid and sodium carbonate
Being 6.5, dissolved oxygen controls 25%, and rotating speed is that 150r/min. ventilation should maintain 0.03Mpa at 600L/min, fermentation tank pressure,
Fermentation time is 16h, obtains product B.
4) prepare products C: adjust the pH to 4.5 of product B, and temperature in fermentation tank is cooled to 2 DEG C, preserve 3 days, then
Temperature is recovered to 30 DEG C, obtains products C；
5) preparing product D: seed liquor Y be inoculated in products C, inoculum concentration is 10%, and fermentation temperature is 31 DEG C, uses Fructus Citri Limoniae
Acid and ammonia regulation reaction pH value be 6.5, dissolved oxygen controls 20%, rotating speed be 200r/min. ventilation at 700L/min, send out
Ferment tank pressure should maintain 0.03Mpa, and fermentation time is 16h, obtains product D.
6) prepare product E: adjust the pH to 4.5 of product D, and temperature in fermentation tank is cooled to 5 DEG C, preserve 5 days, then
Temperature is recovered to 25 DEG C, obtains product E；
7) preparing product F: seed liquor Z be inoculated in product E, inoculum concentration is 15%, and fermentation temperature is 33 DEG C, and regulation is anti-
The pH value answered is 7.3, and dissolved oxygen controls 25%, and rotating speed is that 200r/min. ventilation should maintain at 800L/min, fermentation tank pressure
0.04Mpa, fermentation time is 17h, obtains product F.
8) prepare product G: adjust the pH to 5.0 of product F, and temperature in fermentation tank is cooled to 2 DEG C, preserve 4 days, then
Temperature is recovered to 25 DEG C, obtains product G；
9) marine organisms organic liquid fertilizer (being in terms of 100 parts by product gross weight) is prepared: add in 47 weight portion product G
Enter ammonium sulfate 10 weight portion, potassium sulfate 16 weight portion, ammonium dihydrogen phosphate 12 weight portion, 15 parts of carbamide, be stirred to dissolve uniformly.
Embodiment 2
1) product A is prepared: by clean to fresh Thallus Laminariae (Thallus Eckloniae) and Alga Sgrgassi Enerves (mass ratio is 1:1), remove impurity, after soaking 36h, mill
Slurry, proceeds in fermentation tank, and sterilizing 30min at 125 DEG C is cooled to 30 DEG C and obtains product A, standby.
2) seed liquor is prepared: operational approach is same as in Example 1.
3) preparing product B: seed liquor X be inoculated in product A, inoculum concentration is 15%, and fermentation temperature is 35 DEG C, uses oxalic acid
With sodium acetate regulation reaction pH value be 7.0, dissolved oxygen controls 30%, rotating speed be 200r/min. ventilation at 1000L/min,
Fermentation tank pressure should maintain 0.05Mpa, and fermentation time is 16h, obtains product B.
4) prepare products C: adjust the pH to 4.0 of product B, and temperature in fermentation tank is cooled to 3 DEG C, preserve 4 days, then
Temperature is recovered to 25 DEG C, obtains products C；
5) preparing product D: seed liquor Y be inoculated in products C, inoculum concentration is 15%, and fermentation temperature is 33 DEG C, uses acetic acid
Being 6.8 with the pH value of ammonia regulation reaction, dissolved oxygen controls 25%, and rotating speed is that 200r/min. ventilation is in 800L/min, fermentation
Tank pressure should maintain 0.04Mpa, and fermentation time is 12h, obtains product D.
6) prepare product E: adjust the pH to 4.5 of product D, and temperature in fermentation tank is cooled to 2 DEG C, preserve 3 days, then
Temperature is recovered to 30 DEG C, obtains product E；
7) preparing product F: seed liquor Z be inoculated in product E, inoculum concentration is 15%, and fermentation temperature is 30 DEG C, and regulation is anti-
The pH value answered is 7.2, and dissolved oxygen controls 20%, and rotating speed is that 200r/min. ventilation should maintain at 900L/min, fermentation tank pressure
0.05Mpa, fermentation time is 18h, obtains product F.
8) prepare product G: adjust the pH to 4.0 of product F, and temperature in fermentation tank is cooled to 3 DEG C, preserve 3 days, then
Temperature is recovered to 30 DEG C, obtains product G；
9) marine organisms organic liquid fertilizer (being in terms of 100 parts by product gross weight) is prepared: add in 47 weight portion product G
Enter ammonium sulfate 10 weight portion, potassium sulfate 20 weight portion, ammonium dihydrogen phosphate 8 weight portion, 15 parts of carbamide, be stirred to dissolve uniformly.
Comparative example 1
1) product A is prepared: operational approach is with embodiment 2.
2) preparing seed liquor: only prepare the seed liquor of single culture, described single culture is addicted to sugar bacillus cereus, activation
With the culture medium prescription spread cultivation it is: sweet potato powder 22g, glutamic acid 0.03g, yeast extract 5g, peptone 11g, manganese dioxide 0.02g,
Potassium dihydrogen phosphate 0.02g, iron chloride 0.005g, magnesium sulfate 0.2g, calcium carbonate 0.1g, water 1000mL, regulate pH7.2.Spread cultivation bar
Part is: cultivate 22h for 30 DEG C.Obtain product B.
3) prepare products C: adjust the pH to 4.5 of product B, and temperature in fermentation tank is cooled to 2 DEG C, preserve 3 days, then
Temperature is recovered to 30 DEG C, obtains products C.
4) marine organisms organic liquid fertilizer (being in terms of 100 parts by product gross weight) is prepared: in products C, add ammonium sulfate
10 weight portions, potassium sulfate 20 weight portion, ammonium dihydrogen phosphate 8 weight portion, 15 parts of carbamide, be stirred to dissolve uniformly.
Comparative example 2
1) product A is prepared: operational approach is same as in Example 2.
2) seed liquor is prepared: operational approach is same as in Example 2.
3) product B is prepared: after seed liquor X, seed liquor Y, seed liquor Z being mixed according to mass ratio 1:1:1, be inoculated into product A
In, inoculum concentration is 15%, and fermentation temperature is 35 DEG C, and the pH value regulating reaction with oxalic acid and sodium acetate is 7.0, and dissolved oxygen controls
30%, rotating speed is that 200r/min. ventilation should maintain 0.05Mpa at 1000L/min, fermentation tank pressure, and fermentation time is 20h,
Obtain product B.
4) prepare products C: adjust the pH to 4.5 of product B, and temperature in fermentation tank is cooled to 2 DEG C, preserve 3 days, then
Temperature is recovered to 30 DEG C, obtains products C.
5) marine organisms organic liquid fertilizer (being in terms of 100 parts by product gross weight) is prepared: in products C, add ammonium sulfate
10 weight portions, potassium sulfate 20 weight portion, ammonium dihydrogen phosphate 8 weight portion, 15 parts of carbamide, be stirred to dissolve uniformly.
Product checking: product indices is entered according to People's Republic of China (PRC) agricultural industry criteria NY/T 884-2012
Row detection, testing result is shown in Table 1.
Table 1: the embodiment of the present invention and the quality measurements of comparative example
Result above shows, the microbial manure indices that the present invention produces is obviously higher than national standard, miscellaneous bacteria rate
Substantially reduce, ascarid mortality rate 100%.
Embodiments of the invention and comparative example related fertilizer impose on Fructus Solani melongenae plantation plot, and mu consumption is 40kg.Planting season
Being 1 year, contrast with using the similar products bought in market, fertilizer efficiency the results are shown in Table 2.
Table 2: fertilizer efficiency comparing result
Result shows: in the case of dose is suitable, uses the marine organisms organic liquid fertilizer product of the present invention, hence it is evident that
Promote crop quality, greatly reduce effect pest and disease damage, effectively strengthen the resistance of crop, can raising effect yield 10-12%；
No matter showing from product quality and fertilizer efficiency, the composite ratings fermentation technique effect that the present invention uses is significantly better than
Single culture fermentation and common multiple bacteria compound fermentation.
Preserving number addicted to sugar bacillus cereus YIC～Alg3 of the present invention is CGMCC No.12155, depositary institution's title:
China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution address: BeiChen West Road, Chaoyang District, BeiJing City 1
Number institute 3, preservation date: on 03 02nd, 2016, Latin Classification And Nomenclature: Bacillus halosaccharovorans.
The described biological nature addicted to sugar bacillus cereus YIC～Alg3 is as follows:
After YIC～Alg3 bacterial strain grows 48h on alginic acid sole carbon source flat board, bacterium colony is irregular, and faint yellow, centre is not
Transparent, edge is translucent, surface folding.It is shaft-like that microscope observes bacterial strain, can produce spore, gram at end or proximal end
The positive, can move, and Later growth thalline easily assembles agglomerating (Fig. 1).Growth temperature range 20～45 DEG C, PH scope 6～9, NaCl
Concentration range 0～15%, bacterial strain optimum growth temperature 37 DEG C, the most suitable growth pH 7.5.Catalase, oxidase, VP tests the positive, energy
Enough utilize glucose, mannose, mannitol, fructose, lactose, galactose, starch；Can not utilize sorbitol, cellulose,.Bacterial strain
16SrDNA sequence sees appendix 1, and phylogenetic tree is as shown in Figure 2.According to its physiological and biochemical property, 16SrDNA sequence and system are sent out
Educate tree information, be addicted to sugar bacillus halosaccharovorans by this identification of strains.
Note :+represent and can utilize ,～represent and can not utilize.
Bacillus cereus of the present invention is known commodity, its Classification system: Bacillus
cereus.Frankland。
Bacillus amyloliquefaciens of the present invention is known commodity, its Classification system: Bacillus
amyloliquefaciens。
Claims (8)
1. the method that a compound microorganism ferments prepares marine organisms organic liquid fertilizer, it is characterised in that include following step
Rapid:
1) product A is prepared: fresh marine algae plant is clean, remove impurity, after soaking 12-48h, defibrination, proceed in fermentation tank,
110-150 DEG C of sterilizing 15-30min, is cooled to 20-35 DEG C and obtains product A；
2) seed liquor is prepared: activated by bacillus cereus and spread cultivation, preparing bacillus cereus seed liquor X；Will be addicted to sugar spore
Bacillus activates and spreads cultivation, and prepares addicted to sugar bacillus cereus seed liquor Y；Being activated by bacillus amyloliquefaciens and spread cultivation, preparation solves starch
Bacillus cereus seed liquor Z；
3) preparing product B: seed liquor X be inoculated in product A, seed liquor X consumption is the 10%-20% of product A quality, fermentation temperature
Degree, for 30-37 DEG C, is 5-8 with the pH value of acid-base modifier regulation reaction, and dissolved oxygen controls at 10-40%, and rotating speed is 100-250r/
Min, ventilation should maintain 0.02-0.08Mpa at 500-2000L/min, fermentation tank pressure, and fermentation time is 8-24h, is produced
Product B；
4) prepare products C: adjust the pH to 4.0～5.5 of product B, and temperature in fermentation tank is cooled to 0 DEG C～5 DEG C, preserve 2
～5 days, then temperature is recovered to 20 DEG C～35 DEG C, obtain products C；
5) preparing product D: seed liquor Y be inoculated in products C, seed liquor Y consumption is the 10%-20% of products C quality, fermentation temperature
Degree, for 30-34 DEG C, is 5-8 with the pH value of acid-base modifier regulation reaction, and dissolved oxygen controls at 10-40%, and rotating speed is 100-250r/
Min, ventilation should maintain 0.02-0.08Mpa at 500-2000L/min, fermentation tank pressure, and fermentation time is 8-24h, is produced
Product D；
6) prepare product E: adjust the pH to 4.0～5.5 of product D, and temperature in fermentation tank is cooled to 0 DEG C～5 DEG C, preserve 2
～7 days, then temperature is recovered to 25 DEG C～35 DEG C, obtain product E；
7) preparing product F: seed liquor Z be inoculated in product E, seed liquor Z consumption is the 10%-20% of product E quality, fermentation temperature
Degree, for 30-34 DEG C, is 5-8 with the pH value of acid-base modifier regulation reaction, and dissolved oxygen controls at 10-40%, and rotating speed is 100-250r/
Min, ventilation should maintain 0.02-0.08Mpa at 500-2000L/min, fermentation tank pressure, and fermentation time is 8-24h, is produced
Product F；
8) prepare product G: adjust the pH to 4.0～5.5 of product F, and temperature in fermentation tank is cooled to 0 DEG C～5 DEG C, preserve 2
～7 days, then temperature is recovered to 25 DEG C～35 DEG C, obtain product G；
9) marine organisms organic liquid fertilizer is prepared: in product G, add ammonium sulfate 10-15 weight portion, potassium sulfate 8-20 weight
Part, ammonium dihydrogen phosphate 5-15 weight portion, carbamide 10-15 part, be stirred to dissolve that uniformly to obtain the marine organisms of 100 weight portions organic
Liquid fertilizer.
2. the method that compound microorganism ferments as claimed in claim 1 prepares marine organisms organic liquid fertilizer, its feature exists
In: the marine algae plant in described step 1) is the one in Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.), Thallus Laminariae (Thallus Eckloniae), Brown algae, sargassum thunbergii and Alga Sgrgassi Enerves or appoints
Meaning ratio two or more.
3. the method that compound microorganism ferments as claimed in claim 1 prepares marine organisms organic liquid fertilizer, its feature exists
In described step 2) in bacillus cereus activation with the culture medium prescription spread cultivation be: bean cake powder 20g, yeast extract 6g, extra large
Sodium alginate 1.2g, bovine bile 0.05g, potassium dihydrogen phosphate 0.04g, sodium chloride 0.2g, magnesium sulfate 0.2g, mannitol 9.5g, water
1000mL, regulates pH6.8-7.0, and the condition of spreading cultivation is: cultivate 20h for 33 DEG C.
4. the method that compound microorganism ferments as claimed in claim 1 prepares marine organisms organic liquid fertilizer, its feature exists
In described step 2) in addicted to sugar bacillus cereus activation with the culture medium prescription spread cultivation be: sweet potato powder 22g, glutamic acid
0.03g, yeast extract 5g, peptone 11g, manganese dioxide 0.02g, potassium dihydrogen phosphate 0.02g, iron chloride 0.005g, magnesium sulfate
0.2g, calcium carbonate 0.1g, water 1000mL, regulate pH7.2-7.5, and the condition of spreading cultivation is: cultivates 22h for 30 DEG C.
5. the method that compound microorganism ferments as claimed in claim 1 prepares marine organisms organic liquid fertilizer, its feature exists
In described step 2) in bacillus amyloliquefaciens activation with the culture medium prescription spread cultivation be: starch 20g, bean cake 40g, ferment
Female cream 6g, potassium dihydrogen phosphate 2.0g, sodium acetate 5.0g, manganese sulfate 0.5g, magnesium sulfate 0.5g, calcium carbonate 0.3g, water 1000ml, adjust
Joint pH6.8-7.0, the condition of spreading cultivation is: cultivate 24h for 35 DEG C.
6. the method that compound microorganism ferments as claimed in claim 1 prepares marine organisms organic liquid fertilizer, its feature exists
In: the acid-base modifier in described step 3) to step 8) is citric acid, oxalic acid, acetic acid, dipotassium hydrogen phosphate, sodium carbonate, vinegar
One or more in acid sodium.
7. the method that compound microorganism ferments as claimed in claim 1 prepares marine organisms organic liquid fertilizer, its feature exists
In: the living bacteria count of the marine organisms organic liquid fertilizer in described step 9) is 1,500,000,000 more than CFU/ml, and pH value is
6.5-7.0, N, P, K content ratio are 1-2:1:0.8-1.5.
8. the method that compound microorganism ferments as claimed in claim 1 prepares marine organisms organic liquid fertilizer, its feature exists
In: described addicted to sugar bacillus cereus be addicted to sugar bacillus cereus YIC～Alg3, its preserving number is CGMCC No12155.
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