CN106348867A - Method for preparing medium element liquid water-soluble fertilizer by seaweed combined hierarchical fermentation - Google Patents
Method for preparing medium element liquid water-soluble fertilizer by seaweed combined hierarchical fermentation Download PDFInfo
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- CN106348867A CN106348867A CN201610742214.5A CN201610742214A CN106348867A CN 106348867 A CN106348867 A CN 106348867A CN 201610742214 A CN201610742214 A CN 201610742214A CN 106348867 A CN106348867 A CN 106348867A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C05C5/04—Fertilisers containing other nitrates containing calcium nitrate
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention relates to a method for preparing medium element liquid water-soluble fertilizer by seaweed combined hierarchical fermentation; dried seaweed is ground into powder that is loaded in a fermentation pot for sterilizing; seed liquids of two bacteria are prepared respectively, the seed liquids are inoculated to seaweed stock in multiple phases such that hierarchical fermentation is achieved; after fermentation, seaweed liquid is obtained by press filtering, and the seaweed liquid and medium elements are proportionally added into a stirring kettle and are stirred and fully dissolved to obtain the medium element liquid water-soluble fertilizer with seaweed liquid; the combined hierarchical fermentation technique used herein is more effective than single bacterium fermentation and common multi-bacterium mixed fermentation, with high fermentation efficiency and low time consumption; the whole process is highly operable, green and energy-saving, and is environment-friendly; the fertilizer combines medium elements with the effective ingredients of seaweed, and the fertilizer is significantly effective in improving stress tolerance and disease resistance in crops and can meet the needs of modern agriculture.
Description
Technical field
The present invention relates to a kind of production method of moderate-element liquid Water soluble fertilizer.
Background technology
Marine alga fertilizer, with natural seaweed as raw material, is processed through special biochemical process, extracts and retain marine alga natural activity
Composition, a kind of novel organic fertilizer material that refine concentrates and formed.Marine alga fertilizer has raising crop yield and quality, resists disease pest
Effect that evil and poor environment and improved soil conserve water and soil, it is applied to agricultural production the huge market demand and good
Development prospect.But, the quality of marine alga fertilizer to be ensured, it is necessary for getting hold of Sargassum raw material and the crucial ring of extraction process two
Section.
First, the quality of marine alga fertilizer quality depends on raw material used.At present, the raw material used by alga fertilizer manufacturing enterprise
Mainly based on Brown algae, including yellow tang, Thallus Laminariae (Thallus Eckloniae), Thallus Laminariae (Thallus Eckloniae), Alga Sgrgassi Enervess etc..Other algae such as Fucus Vesiculosus, Entermorpha, ulva, Kappa
Zao Dengyeyou minority enterprise with.External alga fertilizer enterprise is most to be raw material using Brown algae, is wherein especially applied with yellow tang
The most extensive such as famous brand name such as seasol, maxicrop, practical function effect also indicates that yellow tang is the Sargassum raw material of high-quality.
But, China coast does not have yellow tang to be distributed, and the raw material type that domestic enterprise adopts is numerous and diverse, and quality is irregular to differ, and causes Sargassum
Fertilizer product quality is very different, or even some producers are turned on the camouflage of " alga fertilizer ", directly blends alginic acid, fulvic acid, nitrogen phosphorus
Potassium etc. pretends to be alga fertilizer, have impact on alga fertilizer quality, has upset alga fertilizer market.
Secondly, the second factor of decision alga fertilizer quality is extraction process.Different extraction processes is to alga fertilizer activity
The species and content impact of composition is huge.At present, the extracting method of Sargassum active component mainly has chemical method, Physical and life
Thing method.Chemical method mainly uses acid, alkali and organic solvent and processes alginic cell, so that cell is cleared up or endogenous substance solubilising, should
Method is simple to operate, easily realizes, and is also the method that most alga fertilizer manufacturing enterprises adopt both at home and abroad.But, chemistry examination
Agent is sizable to the destruction of the active material ingredients in alginic cell, and the organic reagent remaining is also potential to environment
Harm.The principle of Physical is control pressure, the physical environmental condition such as temperature, so that alginic cell is crushed using mechanical force, interior
Tolerant release.Although the method technique cleaning, environmental friendliness, the requirement for instrument and equipment is harsh, change in reaction conditions
Acutely, relatively costly it is difficult to realize large-scale production.Bioanalysises include two methods of enzymic degradation and fermentable, its master
Principle is wanted to be to destroy the small-molecule substance that alginic cell wall construction macromolecular substances of degrading easily absorb, the party for plant
Method reaction is gentle, product diversification, and whole production process safety and environmental protection is pollution-free, is preferable Sargassum fertilizer production method.
Research using Production by Microorganism Fermentation marine alga fertilizer is relatively fewer, and only several researchs are from multiple micro-
Biology carries out similar compost fermentation to Sargassum raw material or aerobic fermentation is processed, and there is sweat poor controllability, fermentation period is long,
The problems such as target product is indefinite is it is difficult to meet the unification of industrialization production requirements, stable, controlled standard, the quality of product
It is difficult to ensure.
Evaluation index for agricultural micro organism mainly includes the microbial inoculum quantity in the purity and unit mass of microbial inoculum.Cause
This, the bacterial preparation process as prior art is usually: carries out once under the conditions of the temperature of the suitable existence of microorganism and ph
Fermentation technology, is equivalent to hot-house culture.
Because the acidifying of current soil compaction is serious, outside air temperature is changeable, and the microbial bacterial agent of hot-house culture is directly thrown
It is put in soil, the soil environment because not adapting to severe causes mortality, even if having surviving because population cannot be formed on a small quantity
Effect, also cannot play the effect of microbial bacterial agent.
Content of the invention
The technical problem to be solved is to provide a kind of Sargassum composite ratings fermentation and prepares moderate-element liquid water
Molten fertile method, the thermophilic sugar spore of first, the bacillus megaterium of cellulase-producing using function admirable and degraded alginic acid
Bacillus degraded Sargassum, and simulate soil natural ecological environment, make the strain after fermentation after putting into soil, ring can be adapted to quickly
Border is it is ensured that survival rate；Secondth, prepare moderate-element liquid using described bacillus megaterium and thermophilic sugar fermentation of bacillus Sargassum liquid
Body Water soluble fertilizer, in preparation method, sweat is stablized controlled, and fermentation period is short, and product efficiency is notable, is suitable to large-scale production.
In order to solve above-mentioned technical problem, the present invention employs the following technical solutions:
Sargassum composite ratings fermentation prepares the method for moderate-element liquid Water soluble fertilizer it is characterised in that entering according to following steps
OK:
1), prepare marine algae powder: the Sargassum after drying is clayed into power；
2) product a and seed liquor, are prepared: marine algae powder and water load in fermentation tank according to mass ratio 1～10: 100,
Sterilize under the conditions of 100～150 DEG C 15～35min, obtains product a；
Bacillus megaterium is activated and spreads cultivation, prepare bacillus megaterium seed liquor x；By thermophilic sugar bacillus cereuss activate with
Spread cultivation, prepare thermophilic sugar bacillus cereuss seed liquor y；
3), prepare product b: seed liquor x is inoculated in product a, seed liquor x consumption be product a mass 1%～
10%, fermentation temperature is 30～37 DEG C, and the ph value adjusting reaction with acid-base modifier is 5～8, and fermentation time is 8～24h, obtains
To product b；
4), prepare product c: the ph to 4.0～5.5 of adjustment product b, in fermentation tank, temperature, to 0 DEG C～5 DEG C, preserves 2～5
My god, then temperature is adjusted to 20 DEG C～35 DEG C, obtain product c；
5), prepare product d: seed liquor y is inoculated in product c, seed liquor y consumption be product c mass 1%～
10%, fermentation temperature is 30～34 DEG C, and the ph value adjusting reaction with acid-base modifier is 5～8, and fermentation time is 8～24h, obtains
To product d；
6), prepare product e: the ph to 4.0～5.5 of adjustment product d, in fermentation tank, temperature, to 0 DEG C～5 DEG C, preserves 2～7
My god, then temperature is adjusted to 25 DEG C～35 DEG C, obtain product e；
7), prepare seaweed extracted liquor: product e filter pressing is obtained Sargassum slag and filtrate, wherein filtrate is seaweed extracted liquor；
8), prepare moderate-element liquid Water soluble fertilizer: by step 7) seaweed extracted liquor that obtains mixed with moderate-element, stirring
To dissolving completely, obtain moderate-element liquid Water soluble fertilizer；Wherein seaweed extracted liquor and the mass ratio of moderate-element are 40～70: 30
～60.
Step 1) in Sargassum be two kinds of one of Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.), Thallus Laminariae (Thallus Eckloniae), sargassum thunbergii and Alga Sgrgassi Enervess or arbitrary proportion with
On.
Step 2) in bacillus megaterium activation with spread cultivation: fluid medium is made into according to following mass ratio: carboxymethyl
10 parts of sodium cellulosate, 10 parts of tryptone, 3 parts of dipotassium hydrogen phosphate, 3 parts of sodium dihydrogen phosphate, 3 parts of iron chloride, 0.5 part of sodium chloride and
1000 parts of distilled water；The volume ratio of bacillus megaterium yic～bm1 and culture medium is 5: 100, and fermentation temperature is 32 DEG C, uses
The ph value that acid-base modifier adjusts reaction is 6.5, and dissolved oxygen controls 20%, and rotating speed is 200r/min, ventilation in 800l/min,
Fermentation tank pressure should maintain 0.03mpa, and fermentation time is 20h；
Step 2) in thermophilic sugar bacillus cereuss activation with spread cultivation: fluid medium is made into according to following mass ratio: alginic acid
5 parts of sodium, 5 parts of ammonium sulfate, 1 part of magnesium sulfate, 2 parts of dipotassium hydrogen phosphate, 0.01 part of ferrous sulfate and 1000 parts of distilled water；Thermophilic sugar spore
Bacillus is seeded in fluid medium, and the volume ratio of thermophilic sugar bacillus cereuss yic～alg3 and culture medium is 5: 100, fermentation temperature
Spend for 30 DEG C, the ph value adjusting reaction with acid-base modifier is 7.2, dissolved oxygen controls 20%, rotating speed is 200r/min, ventilation
In 800l/min, the tank pressure that ferments should maintain 0.03mpa, and fermentation time is 20h.
Step 2) described in marine algae powder and water mass ratio be 4: 100,115 DEG C of sterilising temp, sterilization time 25min；
Step 4) in, the ph to 5.0 of adjustment product b, in fermentation tank, temperature, to 5 DEG C, preserves 3 days, then adjusts temperature
To 20 DEG C.
Step 6) in, the ph to 5.0 of adjustment product d, in fermentation tank, temperature, to 5 DEG C, preserves 3 days, then adjusts temperature
To 25 DEG C.
Described acid-base modifier is citric acid, oxalic acid, acetic acid, dipotassium hydrogen phosphate, one of sodium carbonate and Sodium Acetate Trihydrate.
Described moderate-element is Magnesium sulfate heptahydrate, four water-calcium nitrate, ethylenediamine tetraacetic acid disodium magnesium salt, ethylenediaminetetraacetic acid two
One of sodium calcium and seven water magnesium nitrates or arbitrary proportion two or more.
Described bacillus megaterium is bacillus megaterium yic～bm1, and preserving number is cgmcc no.12156.
Described thermophilic sugar bacillus cereuss are thermophilic sugar bacillus cereuss yic～alg3, and preserving number is cgmcc no.12155.
Invent and there are following Advantageous Effects:
The huge spore bacterial strain that the present invention adopts has the ability of cellulase-producing, can destroy alginic cell wall construction and promote
More nutrient substance in Sargassum body are made to be released.The thermophilic sugar bacillus cereuss that the present invention adopts have the alignic ability of degraded,
On the one hand alginic acid of can degrading generates the nutritional labeling that brown alga oligose of small-molecular-weight etc. is easy to plant absorption utilization, the opposing party
Face is destroyed alginic cell wall construction and promotes more nutrient substance in Sargassum body to be released.Using this strain fermentation process Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.),
The Sargassum raw material such as sargassum thunbergii, Sargassum, after certain natural law that ferments, using this fermentation liquid as alga fertilizer mother solution, directly or multiple
Join n, p, k, trace element, aminoacid, the effective ingredient such as organic matter is used for crop growth.Fertilizer efficiency experiment shows, using the party
Method obtains fermentation liquid and can be obviously promoted plant root growth, improved soil microenvironment, improves crop yield.
The present invention also has the following characteristics that
1st, the present invention adopts bacillus megaterium yic～bm1 and thermophilic sugar bacillus cereuss yic～alg3 to fermentation of seaweed,
Alginic cell wall construction can be destroyed promotes more nutrient substance in Sargassum body to be released, and sweat stablizes controlled, fermentation
Cycle is short, product efficiency is notable, is suitable to large-scale production.
2nd, the present invention adopts composite bacteria to ferment, and all kinds of bacterial strains play synergism, and effect fertilizer efficiency is significantly better than that single
Strain fermentation, and pure biofermentation is not it is ensured that Sargassum active component is damaged.
3rd, the composite bacteria fermentation that the present invention adopts is different from different strain seed liquor mixed fermentation simply, but
According to the effect of microorganism different, according to precedence successively staged fermentation, it is to avoid vying each other between microorganism species is short of money
Anti-, reduce in-fighting, the effect of respective flora is performed to and maximizes the effect it is ensured that best.
4th, trace element is combined by the present invention with effective ingredient in Sargassum, can significantly improve that crop is degeneration-resistant, disease resistance.It is easy to
Plant absorption utilizes, and Sargassum active component is as a kind of important signaling molecule simultaneously, be capable of involved in plant growth regulating and
Induction resistance mechanism promotes the growth of plant, improves the resistance to poor environment and pest and disease damage for the plant.
5th, the present invention makes the bacterial strain that fermentation goes out possess the ability adapting to severe soil environment by adjusting fermentation condition, has
Performance beneficial to bacterium function.
6th, the present invention, by slow temperature adjustment, makes the change of bacterial strain adaptive temperature, it is to avoid temperature change is too fast, bacterial strain
Do not adapt to and mortality.
Specific embodiment
Further illustrate the present invention with reference to instantiation.
Embodiment 1
Fresh sargassum thunbergii after clean drying is worn into 50～60 mesh powder.
Marine algae powder loads in fermentation tank with water according to mass ratio 4: 100, and sterilize under the conditions of 115 DEG C 25min, is produced
Product a.
And prepare seed liquor:
Seed liquor x prepare example: the bacillus megaterium yic～bm1 of cellulase-producing is seeded to fluid medium,
The volume ratio of bacillus megaterium yic～bm1 and culture medium is 5: 100, and fermentation temperature is 32 DEG C, is adjusted with acid-base modifier
The ph value of reaction is 6.5, and dissolved oxygen controls 20%, and rotating speed is 200r/min, and in 800l/min, the tank pressure that ferments should maintain ventilation
In 0.03mpa, fermentation time is 20h；Spread cultivation by same operating procedure after activation, obtain seed liquor x；
Seed liquor y prepare example: thermophilic sugar bacillus cereuss yic～alg3 is seeded to fluid medium, thermophilic sugar spore bar
The volume ratio of bacterium yic～alg3 and culture medium is 5: 100, and fermentation temperature is 30 DEG C, adjusts the ph of reaction with acid-base modifier
It is worth for 7.2, dissolved oxygen controls 20%, and rotating speed is 200r/min, and in 800l/min, the tank pressure that ferments should maintain ventilation
0.03mpa, fermentation time is 20h；Spread cultivation by same operating procedure after activation, obtain seed liquor y；
Seed liquor x is added in product a, wherein seed liquor x and the mass ratio of product a are 5: 100, and fermentation temperature is 32
DEG C, the ph value adjusting reaction with acid-base modifier is 6.5, and dissolved oxygen controls 20%, and rotating speed is 200r/min, and ventilation exists
800l/min, fermentation tank pressure should maintain 0.03mpa, and fermentation time is 24h, obtains product b；The ph to 5.0 of adjustment product b,
And with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 3 days, product is adjusted with per hour 3 DEG C of temperature change
Temperature, to 20 DEG C, obtains product c；Then supplement seed liquor y, seed liquor y supplemented is 5: 100 with the mass ratio of product c, fermentation
Temperature is 30 DEG C, and the ph value adjusting reaction with acid-base modifier is 7.2, and dissolved oxygen controls 20%, and rotating speed is 200r/min, ventilation
In 800l/min, the tank pressure that ferments should maintain 0.03mpa to amount, and fermentation time is 24h, obtains product d；The ph of adjustment product d is extremely
5.0, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 3 days, adjusted with per hour 3 DEG C of temperature change
The temperature of whole product d, to 25 DEG C, obtains product e；Yic containing bacillus megaterium～bm1 and thermophilic sugar bud will be obtained after product e filter pressing
Spore bacillus yic～alg3 seaweed extracted liquor；By seaweed extracted liquor, Magnesium sulfate heptahydrate and four water-calcium nitrate according to mass ratio 60: 20:
20 mixing, after stirring and dissolving is entirely complete, as moderate-element liquid Water soluble fertilizer.
Embodiment 2
Fresh Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) after clean drying is worn into 50～60 mesh powder；
Marine algae powder loads in fermentation tank with water according to mass ratio 4: 100, and sterilize under the conditions of 115 DEG C 25min, is produced
Product a.
And prepare seed liquor:
Seed liquor x prepare example: the bacillus megaterium yic～bm1 of cellulase-producing is seeded to fluid medium,
The volume ratio of bacillus megaterium yic～bm1 and culture medium is 5: 100, and fermentation temperature is 32 DEG C, is adjusted with acid-base modifier
The ph value of reaction is 6.5, and dissolved oxygen controls 20%, and rotating speed is 200r/min, and in 800l/min, the tank pressure that ferments should maintain ventilation
In 0.03mpa, fermentation time is 24h；Spread cultivation by same operating procedure after activation, obtain seed liquor x；
Seed liquor y prepare example: thermophilic sugar bacillus cereuss yic～alg3 is seeded to fluid medium, thermophilic sugar spore bar
The volume ratio of bacterium yic～alg3 and culture medium is 5: 100, and fermentation temperature is 30 DEG C, adjusts the ph of reaction with acid-base modifier
It is worth for 7.2, dissolved oxygen controls 20%, and rotating speed is 200r/min, and in 800l/min, the tank pressure that ferments should maintain ventilation
0.03mpa, fermentation time is 24h；Spread cultivation by same operating procedure after activation, obtain seed liquor y；
Seed liquor x is added in product a, wherein seed liquor x and the mass ratio of product a are 5: 100, and fermentation temperature is 32
DEG C, the ph value adjusting reaction with acid-base modifier is 6.5, and dissolved oxygen controls 20%, and rotating speed is 200r/min, and ventilation exists
800l/min, fermentation tank pressure should maintain 0.03mpa, and fermentation time is 24h, obtains product b；The ph to 5.0 of adjustment product b,
And with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 3 days, product is adjusted with per hour 3 DEG C of temperature change
Temperature, to 20 DEG C, obtains product c；Then supplement seed liquor y, seed liquor y supplemented is 5: 100 with the mass ratio of product c, fermentation
Temperature is 30 DEG C, and the ph value adjusting reaction with acid-base modifier is 7.2, and dissolved oxygen controls 20%, and rotating speed is 200r/min, ventilation
In 800l/min, the tank pressure that ferments should maintain 0.03mpa to amount, and fermentation time is 24h, obtains product d；The ph of adjustment product d is extremely
5.0, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 3 days, adjusted with per hour 3 DEG C of temperature change
The temperature of whole product d, to 25 DEG C, obtains product e；Yic containing bacillus megaterium～bm1 and thermophilic sugar bud will be obtained after product e filter pressing
Spore bacillus yic～alg3 Sargassum filtrate；Bacterium Sargassum filtrate, ethylenediamine tetraacetic acid disodium magnesium salt, calcium disodium chelate will be contained
Mix according to mass ratio 65: 10: 10: 15 with seven water magnesium nitrates, after stirring and dissolving is entirely complete, as moderate-element liquid Water soluble fertilizer.
Embodiment 3
Fresh Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) after clean drying and new fresh sargassum are compounded according to mass ratio 1: 1, wears into 50～60 mesh powder；
Marine algae powder loads in fermentation tank with water according to mass ratio 4: 100, and sterilize under the conditions of 115 DEG C 25min, is produced
Product a.
And prepare seed liquor:
Seed liquor x prepare example: the bacillus megaterium yic～bm1 of cellulase-producing is seeded to fluid medium,
The volume ratio of bacillus megaterium yic～bm1 and culture medium is 5: 100, and fermentation temperature is 32 DEG C, is adjusted with acid-base modifier
The ph value of reaction is 6.5, and dissolved oxygen controls 20%, and rotating speed is 200r/min, and in 800l/min, the tank pressure that ferments should maintain ventilation
In 0.03mpa, fermentation time is 24h；Spread cultivation by same operating procedure after activation, obtain seed liquor x；
Seed liquor y prepare example: thermophilic sugar bacillus cereuss yic～alg3 is seeded to fluid medium, thermophilic sugar spore bar
The volume ratio of bacterium yic～alg3 and culture medium is 5: 100, and fermentation temperature is 30 DEG C, adjusts the ph of reaction with acid-base modifier
It is worth for 7.2, dissolved oxygen controls 20%, and rotating speed is 200r/min, and in 800l/min, the tank pressure that ferments should maintain ventilation
0.03mpa, fermentation time is 24h；Spread cultivation by same operating procedure after activation, obtain seed liquor y；
Seed liquor x is added in product a, wherein seed liquor x and the mass ratio of product a are 5: 100, and fermentation temperature is 32
DEG C, the ph value adjusting reaction with acid-base modifier is 6.5, and dissolved oxygen controls 20%, and rotating speed is 200r/min, and ventilation exists
800l/min, fermentation tank pressure should maintain 0.03mpa, and fermentation time is 24h, obtains product b；The ph to 5.0 of adjustment product b,
And with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 3 days, product is adjusted with per hour 3 DEG C of temperature change
Temperature, to 20 DEG C, obtains product c；Then supplement seed liquor y, seed liquor y supplemented is 5: 100 with the mass ratio of product c, fermentation
Temperature is 30 DEG C, and the ph value adjusting reaction with acid-base modifier is 7.2, and dissolved oxygen controls 20%, and rotating speed is 200r/min, ventilation
In 800l/min, the tank pressure that ferments should maintain 0.03mpa to amount, and fermentation time is 24h, obtains product d；The ph of adjustment product d is extremely
5.0, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 3 days, adjusted with per hour 3 DEG C of temperature change
The temperature of whole product d, to 25 DEG C, obtains product e；Yic containing bacillus megaterium～bm1 and thermophilic sugar bud will be obtained after product e filter pressing
Spore bacillus yic～alg3 Sargassum filtrate；To contain bacterium Sargassum filtrate ethylenediamine tetraacetic acid disodium magnesium salt, calcium disodium chelate and
Four water-calcium nitrate mixes according to mass ratio 60: 15: 10: 15, after stirring and dissolving is entirely complete, as moderate-element liquid Water soluble fertilizer.
Embodiment 4
Thallus Laminariae (Thallus Eckloniae) after clean drying is worn into 50～60 mesh powder；
Marine algae powder loads in fermentation tank with water according to mass ratio 4:100, and sterilize under the conditions of 115 DEG C 25min, is produced
Product a.
And prepare seed liquor:
Seed liquor x prepare example: the bacillus megaterium yic～bm1 of cellulase-producing is seeded to fluid medium,
The volume ratio of bacillus megaterium yic～bm1 and culture medium is 5: 100, and fermentation temperature is 32 DEG C, is adjusted with acid-base modifier
The ph value of reaction is 6.5, and dissolved oxygen controls 20%, and rotating speed is 200r/min, and in 800l/min, the tank pressure that ferments should maintain ventilation
In 0.03mpa, fermentation time is 24h；Spread cultivation by same operating procedure after activation, obtain seed liquor x；
Seed liquor y prepare example: thermophilic sugar bacillus cereuss yic～alg3 is seeded to fluid medium, thermophilic sugar spore bar
The volume ratio of bacterium yic～alg3 and culture medium is 5: 100, and fermentation temperature is 30 DEG C, adjusts the ph of reaction with acid-base modifier
It is worth for 7.2, dissolved oxygen controls 20%, and rotating speed is 200r/min, and in 800l/min, the tank pressure that ferments should maintain ventilation
0.03mpa, fermentation time is 24h；Spread cultivation by same operating procedure after activation, obtain seed liquor y；
Seed liquor x is added in product a, wherein seed liquor x and the mass ratio of product a are 5: 100, and fermentation temperature is 32
DEG C, the ph value adjusting reaction with acid-base modifier is 6.5, and dissolved oxygen controls 20%, and rotating speed is 200r/min, and ventilation exists
800l/min, fermentation tank pressure should maintain 0.03mpa, and fermentation time is 24h, obtains product b；The ph to 5.0 of adjustment product b,
And with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 3 days, product is adjusted with per hour 3 DEG C of temperature change
Temperature, to 20 DEG C, obtains product c；Then supplement seed liquor y, seed liquor y supplemented is 5: 100 with the mass ratio of product c, fermentation
Temperature is 30 DEG C, and the ph value adjusting reaction with acid-base modifier is 7.2, and dissolved oxygen controls 20%, and rotating speed is 200r/min, ventilation
In 800l/min, the tank pressure that ferments should maintain 0.03mpa to amount, and fermentation time is 24h, obtains product d；The ph of adjustment product d is extremely
5.0, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 3 days, adjusted with per hour 3 DEG C of temperature change
The temperature of whole product d, to 25 DEG C, obtains product e；Yic containing bacillus megaterium～bm1 and thermophilic sugar bud will be obtained after product e filter pressing
Spore bacillus yic～alg3 Sargassum filtrate；Bacterium Sargassum filtrate and four water-calcium nitrate, calcium disodium chelate, ethylenediamine will be contained
Tetraacethyl disodium magnesium and Magnesium sulfate heptahydrate mix according to mass ratio 50: 15: 10: 10: 15, after stirring and dissolving is entirely complete, as middle amount
Element liquid Water soluble fertilizer.
In above-described embodiment, acid regulator is citric acid, and alkali regulator is sodium carbonate.
Further illustrate with reference to experimental data the invention has the advantages that:
The sample collected for Sargassum extraction process of active component different on market, carries out the detection of Sargassum polysaccharides, detection
Result such as table 1.
Table 1 Different Extraction Method gained sample Sargassum polysaccharides content
Microbe fermentation method in this method is utilized to extract the active component of Sargassum, Sargassum in fermentation liquid as can be seen from Table 1
Polyoses content highest, reaction condition gently destroys minimum to Sargassum effective ingredient.Main reason is that two plants of bacterium that fermentation adopts
Can degrade alginic cell wall, so that nutrient substance in frond is released, thermophilic sugar bacillus cereuss have degraded alginic acid life simultaneously
The ability of brown alga oligose becoming small-molecular-weight is so that polysaccharide is higher than all other extraction processs in content and species.
Manure trial data is as follows:
1 materials and methods:
1.1 test sites: Wuzhoufeng Agricultural Technology Co., Ltd. proving ground, neutral brown earth, middle high fertility.Soil nutrient
Situation is: organic 79.3g/kg, alkali-hydrolyzable nitrogen 76.7mg/kg, available phosphoruss 84.6mg/kg, available potassium 80.2mg/kg, ph6.89.
1.2 test materials to be tested: common moderate-element liquid Water soluble fertilizer sample (in addition to being not added with seaweed extracted liquor, make by other
Standby condition is identical with product of the present invention) and product of the present invention；The Semen arachidis hypogaeae of same management.
1.3 EXPERIMENTAL DESIGN:
Process 1 (t1): Sargassum moderate-element liquid Water soluble fertilizer described in embodiment 1；
Process 2 (t2): Sargassum moderate-element liquid Water soluble fertilizer described in embodiment 2；
Process 3 (t3): Sargassum moderate-element liquid Water soluble fertilizer described in embodiment 3；
Process 4 (t4): Sargassum moderate-element liquid Water soluble fertilizer described in embodiment 4；
Control treatment (ck1-4): process that t1-t4 is corresponding to compare common moderate-element liquid Water soluble fertilizer (except being not added with Sargassum
Outside extracting solution, other preparation conditions are identical with product of the present invention).
In addition to experiment fertilizer difference, other agronomy control measures are all consistent for each process.Each processes 3 repetitions, at random
District's groups arrange, and 1kg experiment fertilizer is dissolved separately in 1000kg water, applies 3 times according to the punching of plant growth situation, plot area
100m2, surrounding sets protection row, north-south, line-spacing 45cm, spacing in the rows 15cm.
2 results and analysis
The impact to Semen arachidis hypogaeae character for 2.1 different disposal
The peanut plant fertility shape applying product of the present invention improves significantly compared with matched group, well developed root system, blade ink
Green, cane is sturdy, and resistance strengthens, and disease resistance ability strengthens, and peanut kernels are big.
The impact to peanut yield for 2.2 different disposal
The impact to yield for table 2 different disposal
As can be seen from Table 2, the peanut yield applying product of the present invention significantly improves.
The preserving number of bacillus megaterium yic～bm1 of the present invention is cgmcc no.12156；Depositary institution's title
It is: China Committee for Culture Collection of Microorganisms's common micro-organisms center；Depositary institution address is: the Chaoyang District, Beijing City North Star
West Road 1 institute 3；Preservation date: on March 2nd, 2016；Latin Classification And Nomenclature is: bacillus megaterium.
The biological nature of described bacillus megaterium yic～bm1 is as follows:
After yic～bm1 bacterial strain grows 48h on cellulose culture medium flat plate, bacterium colony is circular, white, surfacing.Micro-
Sem observation bacterial strain is Gram-positive, shaft-like, can move, and produces spore.20～40 DEG C of growth temperature range, ph scope 6-10,
Nacl concentration range 0～20%, 37 DEG C of bacterial strain optimum growth temperature, the most suitable growth ph 7.5.Catalase, oxidase, galactoside
Enzyme positive, indole, vp experiment is negative, does not go back orthonitric acid；Can utilize glucose, mannose, Lactose, Raffinose, sucrose, I
Uncle's sugar, xylose, Mannitol, Sorbitol.According to its physiological and biochemical property, gene order and phylogenetic tree information, by this bacterial strain
It is accredited as bacillus megaterium bacillus megaterium.
The preserving number of thermophilic sugar bacillus cereuss yic～alg3 of the present invention is cgmcc no.12155；Depositary institution's title
It is: China Committee for Culture Collection of Microorganisms's common micro-organisms center；Depositary institution address is: Chaoyang District, Beijing City North Star west
Road 1 institute 3；Preservation date: on March 2nd, 2016；Latin Classification And Nomenclature is: bacillus halosaccharovorans.
The biological nature of described thermophilic sugar bacillus cereuss yic～alg3 is as follows:
After yic～alg3 bacterial strain grows 48h on alginic acid sole carbon source flat board, bacterium colony is irregular, faint yellow, middle impermeable
Bright, edge is translucent, surface folding.Micro- sem observation bacterial strain is shaft-like, can produce spore in end or proximal end, Gram-positive,
Can move, Later growth thalline is easily assembled agglomerating.20～45 DEG C of growth temperature range, ph scope 6～9, nacl concentration range 0～
15%, 37 DEG C of bacterial strain optimum growth temperature, the most suitable growth ph 7.5.Catalase, oxidase, vp test is positive, can utilize glucose,
Mannose, Mannitol, Fructose, Lactose, galactose, starch；Sorbitol, cellulose can not be utilized.According to its physiological and biochemical property, base
Because of sequence and phylogenetic tree information, this identification of strains is thermophilic sugar bacillus cereuss bacillus halosaccharovorans.
Note :+represent and can utilize ,～represent and can not utilize.
Claims (10)
1. the fermentation of Sargassum composite ratings prepares the method for moderate-element liquid Water soluble fertilizer it is characterised in that entering according to following steps
OK:
1), prepare marine algae powder: the Sargassum after drying is clayed into power；
2) product a and seed liquor, are prepared: marine algae powder and water load in fermentation tank according to mass ratio 1～10:100,100～
Sterilize under the conditions of 150 DEG C 15～35min, obtains product a；
Bacillus megaterium is activated and spreads cultivation, prepare bacillus megaterium seed liquor x；Thermophilic sugar bacillus cereuss are activated and expands
Training, prepares thermophilic sugar bacillus cereuss seed liquor y；
3), prepare product b: seed liquor x is inoculated in product a, seed liquor x consumption is the 1%～10% of product a mass, fermentation
Temperature is 30～37 DEG C, and the ph value adjusting reaction with acid-base modifier is 5～8, and fermentation time is 8～24h, obtains product b；
4), prepare product c: the ph to 4.0～5.5 of adjustment product b, in fermentation tank, temperature, to 0 DEG C～5 DEG C, preserves 2～5 days, so
Afterwards temperature is adjusted to 20 DEG C～35 DEG C, obtain product c；
5), prepare product d: seed liquor y is inoculated in product c, seed liquor y consumption is the 1%～10% of product c mass, fermentation
Temperature is 30～34 DEG C, and the ph value adjusting reaction with acid-base modifier is 5～8, and fermentation time is 8～24h, obtains product d；
6), prepare product e: the ph to 4.0～5.5 of adjustment product d, in fermentation tank, temperature, to 0 DEG C～5 DEG C, preserves 2～7 days, so
Afterwards temperature is adjusted to 25 DEG C～35 DEG C, obtain product e；
7), prepare seaweed extracted liquor: product e filter pressing is obtained Sargassum slag and filtrate, wherein filtrate is seaweed extracted liquor；
8), prepare moderate-element liquid Water soluble fertilizer: the seaweed extracted liquor that step 7) is obtained is mixed with moderate-element, stir to molten
Solution completely, obtains moderate-element liquid Water soluble fertilizer；Wherein seaweed extracted liquor and the mass ratio of moderate-element are 40 ~ 70:30 ~ 60.
2. the method that moderate-element liquid Water soluble fertilizer is prepared in Sargassum composite ratings fermentation as claimed in claim 1, its feature exists
It is the two or more of one of Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.), Thallus Laminariae (Thallus Eckloniae), sargassum thunbergii and Alga Sgrgassi Enervess or arbitrary proportion in: the Sargassum in step 1).
3. the method that moderate-element liquid Water soluble fertilizer is prepared in Sargassum composite ratings fermentation as claimed in claim 1, its feature exists
In:
Step 2) in bacillus megaterium activation with spread cultivation: fluid medium is made into according to following mass ratio: carboxymethyl cellulose
10 parts of plain sodium, 10 parts of tryptone, 3 parts of dipotassium hydrogen phosphate, 3 parts of sodium dihydrogen phosphate, 3 parts of iron chloride, 0.5 part of sodium chloride and distillation
1000 parts of water；The volume ratio of bacillus megaterium yic～bm1 and culture medium is 5:100, and fermentation temperature is 32 DEG C, uses soda acid
The ph value that regulator adjusts reaction is 6.5, and dissolved oxygen controls 20%, and rotating speed is 200r/min, and ventilation, in 800l/min, ferments
Tank pressure should maintain 0.03mpa, and fermentation time is 20h；
Step 2) in thermophilic sugar bacillus cereuss activation with spread cultivation: fluid medium is made into according to following mass ratio: sodium alginate 5
Part, 5 parts of ammonium sulfate, 1 part of magnesium sulfate, 2 parts of dipotassium hydrogen phosphate, 0.01 part of ferrous sulfate and 1000 parts of distilled water；Thermophilic sugar spore bar
Bacterium is seeded in fluid medium, and the volume ratio of thermophilic sugar bacillus cereuss yic～alg3 and culture medium is 5:100, fermentation temperature
For 30 DEG C, the ph value adjusting reaction with acid-base modifier is 7.2, and dissolved oxygen controls 20%, and rotating speed is 200r/min, and ventilation exists
800l/min, fermentation tank pressure should maintain 0.03mpa, and fermentation time is 20h.
4. the method that moderate-element liquid Water soluble fertilizer is prepared in Sargassum composite ratings fermentation as claimed in claim 1, its feature exists
In step 2) described in the mass ratio of marine algae powder and water be 4:100,115 DEG C of sterilising temp, sterilization time 25min.
5. the method that moderate-element liquid Water soluble fertilizer is prepared in Sargassum composite ratings fermentation as claimed in claim 1, its feature exists
In: in step 4), the ph to 5.0 of adjustment product b, in fermentation tank, temperature, to 5 DEG C, preserves 3 days, then adjusts temperature to 20
℃.
6. the method that moderate-element liquid Water soluble fertilizer is prepared in Sargassum composite ratings fermentation as claimed in claim 1, its feature exists
In: in step 6), the ph to 5.0 of adjustment product d, in fermentation tank, temperature, to 5 DEG C, preserves 3 days, then adjusts temperature to 25
℃.
7. the method that moderate-element liquid Water soluble fertilizer is prepared in Sargassum composite ratings fermentation as claimed in claim 1, its feature exists
In: described acid-base modifier is citric acid, oxalic acid, acetic acid, dipotassium hydrogen phosphate, one of sodium carbonate and Sodium Acetate Trihydrate.
8. the method that moderate-element liquid Water soluble fertilizer is prepared in Sargassum composite ratings fermentation as claimed in claim 1, its feature exists
In: described moderate-element is Magnesium sulfate heptahydrate, four water-calcium nitrate, ethylenediamine tetraacetic acid disodium magnesium salt, disodiumedetate
One of calcium and seven water magnesium nitrates or arbitrary proportion two or more.
9. moderate-element liquid Water soluble fertilizer is prepared in the Sargassum composite ratings fermentation as described in any one of claim 1 to 8
Method it is characterised in that: described bacillus megaterium is bacillus megaterium yic～bm1, preserving number be cgmcc
no.12156.
10. moderate-element liquid Water soluble fertilizer is prepared in the Sargassum composite ratings fermentation as described in any one of claim 1 to 8
Method it is characterised in that: described thermophilic sugar bacillus cereuss be thermophilic sugar bacillus cereuss yic～alg3, preserving number be cgmcc
no.12155.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618872A (en) * | 2019-01-15 | 2019-04-16 | 五洲丰农业科技有限公司 | A kind of reusable cultivation matrix |
| CN110256138A (en) * | 2019-04-17 | 2019-09-20 | 青岛明月蓝海生物科技有限公司 | A kind of clear liquid type water-soluble fertilizer and preparation method thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85108913A (en) * | 1985-11-04 | 1987-05-20 | 生物有机体公司 | Microbial plant growth promoter and yield enhancer |
| CN102174430A (en) * | 2010-12-31 | 2011-09-07 | 中国海洋大学生物工程开发有限公司 | Bacillus psychrosaccharolyticus strain and application thereof in complex microorganism seaweed fertilizer |
| CN102372517A (en) * | 2011-08-15 | 2012-03-14 | 上海理工大学 | Preparation method and application method of seaweed industrial solid waste organic fertilizer |
| CN102391042A (en) * | 2011-08-03 | 2012-03-28 | 青岛丰泰海洋生物科技有限公司 | Production method of seaweed biological liquid fertilizer |
| CN104262008A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation |
| CN104447008A (en) * | 2014-12-05 | 2015-03-25 | 中国科学院烟台海岸带研究所 | Method for preparing liquid seaweed fertilizer |
| CN104446767A (en) * | 2014-11-17 | 2015-03-25 | 苏州市新巷农艺科技园 | Method for preparing seaweed-containing special fertilizer for flowers and plants |
| CN104692880A (en) * | 2013-12-04 | 2015-06-10 | 青岛根源生物技术集团有限公司 | Method for treating alga through microbial fermentation to produce alga fertilizers |
-
2016
- 2016-08-29 CN CN201610742214.5A patent/CN106348867A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85108913A (en) * | 1985-11-04 | 1987-05-20 | 生物有机体公司 | Microbial plant growth promoter and yield enhancer |
| CN102174430A (en) * | 2010-12-31 | 2011-09-07 | 中国海洋大学生物工程开发有限公司 | Bacillus psychrosaccharolyticus strain and application thereof in complex microorganism seaweed fertilizer |
| CN102391042A (en) * | 2011-08-03 | 2012-03-28 | 青岛丰泰海洋生物科技有限公司 | Production method of seaweed biological liquid fertilizer |
| CN102372517A (en) * | 2011-08-15 | 2012-03-14 | 上海理工大学 | Preparation method and application method of seaweed industrial solid waste organic fertilizer |
| CN104692880A (en) * | 2013-12-04 | 2015-06-10 | 青岛根源生物技术集团有限公司 | Method for treating alga through microbial fermentation to produce alga fertilizers |
| CN104262008A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation |
| CN104446767A (en) * | 2014-11-17 | 2015-03-25 | 苏州市新巷农艺科技园 | Method for preparing seaweed-containing special fertilizer for flowers and plants |
| CN104447008A (en) * | 2014-12-05 | 2015-03-25 | 中国科学院烟台海岸带研究所 | Method for preparing liquid seaweed fertilizer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618872A (en) * | 2019-01-15 | 2019-04-16 | 五洲丰农业科技有限公司 | A kind of reusable cultivation matrix |
| CN110256138A (en) * | 2019-04-17 | 2019-09-20 | 青岛明月蓝海生物科技有限公司 | A kind of clear liquid type water-soluble fertilizer and preparation method thereof |
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