CN106187615B - Method for producing water-soluble fertilizer containing glycotropic bacillus and amino acid - Google Patents
Method for producing water-soluble fertilizer containing glycotropic bacillus and amino acid Download PDFInfo
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- CN106187615B CN106187615B CN201610574457.2A CN201610574457A CN106187615B CN 106187615 B CN106187615 B CN 106187615B CN 201610574457 A CN201610574457 A CN 201610574457A CN 106187615 B CN106187615 B CN 106187615B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention relates to a method for producing water-soluble fertilizer containing glycotropic bacillus and amino acid. Dried seaweed is ground into powder, the seaweed powder is added to water according to a certain proportion, and the mixture is placed in a fermentation tank and then sterilized at a certain temperature for a certain period of time; a seed solution is prepared; a fermentation strain YIC-ALG3 glycotropic bacillus capable of degrading alginic acid is obtained through screening, and a liquid culture medium is then inoculated with the fermentation strain for culture lasting a certain period of time; the seed solution is added into the fermentation tank according to a certain mass ratio to be fermented under a certain condition, the fermentation condition is adjusted after fermentation, the fermentation condition is adjusted again and the seed solution is supplemented after a certain period time of storage, glycotropic bacillus is obtained after three times of adjustment, and glycotropic bacillus and organic matter containing amino acid are evenly stirred to obtain the water-soluble fertilizer containing glycotropic bacillus and amino acid. The fermentation process is stable and controllable, fermentation period is short, product functions are remarkable, and the fertilizer is suitable for large-scale production.
Description
Technical field
The present invention relates to a kind of production method of the Water soluble fertilizer containing thermophilic sugared bacillus and amino acid.
Background technology
Marine alga fertilizer is processed with natural seaweed as raw material by special biochemical process, extracts and retain marine alga natural activity
Composition, a kind of novel organic fertilizer material that refining is concentrated and formed.Marine alga fertilizer has raising crop yield and quality, resists disease pest
Evil and effect for conserving water and soil of poor environment and improved soil, it is applied to agricultural production the huge market demand and good
Development prospect.But, the quality of marine alga fertilizer is ensured, must just get hold of marine alga raw material and the crucial ring of extraction process two
Section.
First, the quality of marine alga fertilizer quality depends on raw material used.At present, the raw material used by alga fertilizer manufacturing enterprise
Mainly based on brown alga, including yellow tang, kelp, sea-tangle, sargassum etc..Other algae such as bladder-wrack, Enteromorpha, sea lettuce, Kappa
Algae etc. also have a small number of enterprises with.It is raw material using brown alga that external alga fertilizer enterprise is most, wherein being applied with yellow tang especially
Such as Seasol, Maxicrop famous brand name the most extensive, practical function effect also indicates that yellow tang is the marine alga raw material of high-quality.
But, China coast does not have yellow tang to be distributed, and the raw material type that domestic enterprise uses is numerous and diverse, and quality is irregular to differ, and causes marine alga
Fertilizer product quality is very different, or even some producers are turned on the camouflage of " alga fertilizer ", directly blends alginic acid, fulvic acid, nitrogen phosphorus
Potassium etc. pretends to be alga fertilizer, have impact on alga fertilizer quality, has upset alga fertilizer market.
Secondly, the second factor for determining alga fertilizer quality is extraction process.Different extraction processes is to alga fertilizer activity
The species and content influence of composition is huge.At present, the extracting method of marine alga active component mainly has chemical method, Physical and life
Thing method.Chemical method mainly uses acid, alkali and organic solvent treatment alginic cell, cell is cleared up or endogenous substance solubilising, should
Method is simple to operate, easily realizes, is also the method that overwhelming majority alga fertilizer manufacturing enterprise uses both at home and abroad.But, chemistry examination
Destruction of the agent to the active material ingredients in alginic cell is sizable, and the organic reagent for remaining is also potential to environment
Harm.The principle of Physical is control pressure, the physical environmental condition such as temperature, and alginic cell is crushed using mechanical force, interior
Tolerant release.Although the method technique cleaning, it is environment-friendly, the requirement for instrument and equipment is harsh, change in reaction conditions
Acutely, it is relatively costly, it is difficult to realize large-scale production.Bioanalysis includes two methods of enzymic degradation and microbial fermentation, its master
It is the small-molecule substance that destruction alginic cell wall construction and macromolecular substances of degrading easily are absorbed for plant, the party to want principle
Method reaction is gentle, product diversification, and whole production process safety and environmental protection is pollution-free, is preferable marine alga fertilizer production method.
Research using Production by Microorganism Fermentation marine alga fertilizer is relatively fewer, and only several research is from various micro-
It is biological that similar compost fermentation or aerobic fermentation treatment are carried out to marine alga raw material, there is fermentation process poor controllability, fermentation period is long,
The problems such as target product is indefinite, it is difficult to meet the unification of industrialization production requirements, stabilization, controllable standard, the quality of product
It is difficult to ensure.
For agricultural micro organism evaluation index mainly including microbial inoculum purity and unit mass in microbial inoculum quantity.Cause
This, the bacterial preparation process as prior art is usually：Carried out once under the conditions of the temperature and pH that microorganism suitably survives
Zymotechnique, equivalent to hot-house culture.
Because the acidifying of current soil hardening is serious, outside air temperature is changeable, and the microbial bacterial agent of hot-house culture is directly thrown
Be put into soil, because not adapting to severe soil environment caused by mortality, even if having surviving because population cannot be formed on a small quantity
Effect, cannot also play the effect of microbial bacterial agent.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of Water soluble fertilizer containing thermophilic sugared bacillus and amino acid
Production method, first, using function admirable for alignic thermophilic sugared bacillus degraded marine alga of degrading, and simulate soil from
Right ecological environment, makes the thermophilic sugared bacillus after fermentation after soil is put into, and can quickly adapt to environment, it is ensured that survival rate；Secondth,
There is provided using the thermophilic sugared bacillus prepare thermophilic sugared bacillus and containing thermophilic sugared bacillus containing amino acid Water soluble fertilizer
Method, the method fermentation process stablizes controllable, and fermentation period is short, and product efficiency significantly, is suitable to large-scale production.
In order to solve the above-mentioned technical problem, the present invention uses following technical scheme：
One kind contains thermophilic sugared bacillus (Latin Classification And Nomenclature：Bacillus halosaccharovorans) and ammonia
The production method of the Water soluble fertilizer of base acid, it is characterised in that follow the steps below：
Amino acid organic matter and thermophilic sugared gemma bacillus agent will be contained according to mass ratio 40~99:After 1~60 mixes, contained
There is the Water soluble fertilizer of thermophilic sugared bacillus and amino acid；
Wherein organic matter containing amino acid be extract the resultant product after monosodium glutamate, the one kind in sodium glutamate and potassium fulvate or
Arbitrary proportion it is two or more；
Wherein thermophilic sugared gemma bacillus agent is prepared from according to following steps：
1) marine algae powder, is prepared：Marine alga after drying is clayed into power；
2) product A, is prepared：Marine algae powder is with water according to mass ratio 1~10:100 are fitted into fermentation tank, at 100~150 DEG C
Under the conditions of sterilize 15~35min, obtain product A；
And prepare seed liquor：Thermophilic sugared bacillus is seeded to fluid nutrient medium culture 20~30 hours；
Volume ratio between thermophilic sugared bacillus and culture medium is 1~10:100；
3) product B, is prepared：Seed liquor is added to product B is obtained in product A, wherein the mass ratio of seed liquor and product A
It is 1~10:100；
4) products C, is prepared：It is 20 DEG C~40 DEG C in temperature, product B is fermented under conditions of pH5~9, during fermentation
Between be 6~10 days, obtain products C；
5) product D, is prepared：The pH to 5.0~6.0 of products C is adjusted, temperature preserves 3h~72h to 3~10 DEG C, is produced
Product D；
6) product E, is prepared:To 20 DEG C~40 DEG C, then pH to 5~9 supplements seed liquor to the temperature of adjustment product D, supplement
The mass ratio of seed liquor and product D be 1~10:100,3h~72h is preserved, obtain product E；
7) product F, is prepared：The pH to 5.0~6.0 of product E is adjusted, temperature preserves 3h~72h to 3~10 DEG C, is produced
Product F；
8) product G, is prepared：The temperature of product F is adjusted to 20 DEG C~40 DEG C, then pH to 5~9 supplements seed liquor, supplement
The mass ratio of seed liquor and product F be 1~10:100,3h~72h is preserved, obtain product G；
9) thermophilic sugared gemma bacillus agent, is prepared：Thermophilic sugared gemma bacillus agent is obtained after product G is air-dried；
Wherein thermophilic sugared bacillus, the referred to as YIC~Alg3 of its depositary institution, its preserving number is CGMCC No12155.
The production method of the Water soluble fertilizer as claimed in claim 1 containing thermophilic sugared bacillus and amino acid, its feature exists
In：PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：5 parts of sodium alginate, 5 parts of ammonium sulfate, sulfuric acid
The mixture of 1000 parts of 1 part of magnesium, 2 parts of dipotassium hydrogen phosphate, 0.01 part of ferrous sulfate, 20 parts of agar and distilled water.
The production method of the Water soluble fertilizer as claimed in claim 1 containing thermophilic sugared bacillus and amino acid, its feature exists
In step 2) mass ratio of the marine algae powder and water is 4:100；115 DEG C of sterilising temp, sterilization time 25min；Prepare seed
During liquid, thermophilic sugared bacillus is seeded in fluid nutrient medium, is cultivated 22 hours, obtains seed liquor, thermophilic sugared bacillus and culture
The volume ratio of base is 5:100；Step 5) and step 7) in, temperature is 5 DEG C, and the holding time is 12h.
The production method of the Water soluble fertilizer as claimed in claim 1 containing thermophilic sugared bacillus and amino acid, its feature exists
In：Step 4), step 6) and step 8) in, temperature be 25 DEG C, pH be 6.5~7.0；Step 4) fermentation time be 8 days；Step 6)
With step 8) holding time be 12h；Step 6) in fermentation seed liquid and product D mass ratio and step 8) in fermentation seed liquid
Mass ratio with product F is 5:100.
The production method of the Water soluble fertilizer as claimed in claim 1 containing thermophilic sugared bacillus and amino acid, its feature exists
In：Step 5), step 6) step 7) and step 8) in, adjustment temperature be per hour adjust 3 DEG C.
The production method of the Water soluble fertilizer as claimed in claim 1 containing thermophilic sugared bacillus and amino acid, its feature exists
In：The marine alga refers to sargassum thunbergii, the one kind or arbitrary proportion in Sargassum horneri and sargassum it is two or more.
Invention has following Advantageous Effects：
The present invention obtains one plant of thermophilic sugared bacillus of marine source by screening, and the bacterial strain has the alignic energy of degraded
Power, brown alga oligose of the alginic acid that on the one hand can degrade generation small-molecular-weight etc. are easy to the nutritional ingredient that plant absorption is utilized；Separately
On the one hand destruction alginic cell wall construction promotes more nutriments in marine alga body to be released.Copper is processed using the strain fermentation
The Sargassum marine alga raw material such as algae, sargassum thunbergii, be can detect in zymotic fluid after the certain number of days of fermenting molecular weight 4000Da with
Under, the degree of polymerization for 2-20 brown alga oligose, using this zymotic fluid as alga fertilizer mother liquor, directly or compounding N, P, K, micro unit
The active ingredients such as element, amino acid, organic matter are used for crop growth.Fertilizer efficiency experiment shows, using the method acquisition fermentation liquid energy
Plant root growth is enough obviously promoted, improved soil microenvironment improves crop yield.
The present invention also has the characteristics that：
1st, the thermophilic sugared bacillus YIC~Alg3 of the marine source obtained using the present invention is to fermentation of seaweed, fermentation process
Stablize controllable, fermentation period is short, product efficiency significantly, is suitable to large-scale production.
2nd, the enzymolysis product of the thermophilic sugared bacillus that the present invention is obtained has good water solubility, it is easy to which plant absorption is utilized, together
A kind of important signaling molecules of Shi Zuowei, the growth regulating and induction resistance mechanism for being capable of involved in plant promotes the growth of plant,
Plant is improved to poor environment and the resistance of pest and disease damage.
3rd, the present invention makes the change of thermophilic sugared bacillus adaptive temperature, it is to avoid temperature change by slow temperature adjustment
Too fast, thermophilic sugared bacillus does not adapt to and mortality.
4th, the thermophilic sugared bacillus that obtains of the present invention is with decomposing outside marine algae extract feature, also with stronger environment
Adaptability, in the soil for putting into acidifying and in the variable environment of temperature change, can quickly adapt to environment survival, play micro- life
The effect of thing microbial inoculum.
Brief description of the drawings
Fig. 1 is the Gram's staining of microexamination YIC~Alg3 bacterial strains and assembles agglomerating phenomenon figure.
Fig. 2 is the YIC~Alg3 bacterial strain phylogenetic tree schematic diagrames based on 16SrDNA sequence constructs.
Specific embodiment
The present invention is further illustrated with reference to instantiation.
Embodiment 1
Fresh sargassum thunbergii after by clean drying wears into 50~60 mesh powder；
Marine algae powder is with water according to mass ratio 2:100 are fitted into fermentation tank, and sterilize 35min under the conditions of 115 DEG C, is produced
Product A；And prepare seed liquor：Thermophilic sugared bacillus YIC~Alg3 is seeded to fluid nutrient medium culture 20 hours；The thermophilic sugared bud
The preserving number of spore bacillus YIC~Alg3 is CGMCC No12155；The fluid nutrient medium is：Sodium alginate 5g, ammonium sulfate 5g,
The mixture of magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar 20g and distilled water 1000ml, pH7.2~7.4；
The volume ratio of thermophilic sugared bacillus YIC~Alg3 and culture medium is 2:100.Seed liquor is added in product A, wherein seed
Liquid is 1 with the mass ratio of product A:100, obtain product B；It is 20 DEG C in temperature, product B is carried out under conditions of pH5.5~6.0
Fermentation, fermentation time is 7 days, obtains products C；The pH of products C is adjusted to 5.0~6.0, and with 20 DEG C per hour of temperature change
Adjustment temperature preserves 24h to 5 DEG C, obtains product D；With the temperature of 20 DEG C per hour of temperature change adjustment product D to 20 DEG C,
PH to 5.5~6.0, then supplements seed liquor, and the seed liquor of supplement is 1 with the mass ratio of product D:100,24h is preserved, produced
Product E；The pH of product E is adjusted to 5.0~6.0, and temperature is adjusted to 5 DEG C with 20 DEG C per hour of temperature change, preserved
24h, obtains product F；The temperature of product F is adjusted to 20 DEG C with 20 DEG C per hour of temperature change, then pH to 5.5~6.0 mends
Seed liquor is filled, the seed liquor of supplement is 1 with the mass ratio of product F:100,24h is preserved, obtain product G；After product G is air-dried
To thermophilic sugared gemma bacillus agent；Amino acid organic matter and thermophilic sugared gemma bacillus agent will be contained according to mass ratio 96:After 4 mix, obtain
To the Water soluble fertilizer containing thermophilic sugared bacillus and amino acid.
Embodiment 2
Fresh Sargassum horneri and new fresh sargassum after by clean drying is according to mass ratio 1:1 compounding, wears into 50~60 mesh powder；
Marine algae powder is with water according to mass ratio 4:100 are fitted into fermentation tank, and sterilize 25min under the conditions of 115 DEG C, is produced
Product A；And prepare seed liquor：Thermophilic sugared bacillus YIC~Alg3 is seeded to fluid nutrient medium culture 22 hours；The thermophilic sugared bud
The preserving number of spore bacillus YIC~Alg3 is CGMCC No12155；The fluid nutrient medium is：Sodium alginate 5g, ammonium sulfate 5g,
The mixture of magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar 20g and distilled water 1000ml, pH7.2~7.4；
The volume ratio of thermophilic sugared bacillus YIC~Alg3 and culture medium is 5:100.Seed liquor is added in product A, wherein seed
Liquid is 5 with the mass ratio of product A:100, obtain product B；It is 25 DEG C in temperature, product B is carried out under conditions of pH6.5~7.0
Fermentation, fermentation time is 8 days, obtains products C；The pH of products C is adjusted to 5.0~6.0, and with 3 DEG C per hour of temperature change
Adjustment temperature preserves 12h to 5 DEG C, obtains product D；With the temperature of 3 DEG C per hour of temperature change adjustment product D to 25 DEG C, pH
To 6.5~7.0, seed liquor is then supplemented, the seed liquor of supplement is 5 with the mass ratio of product D:100,12h is preserved, obtain product
E；The pH of product E is adjusted to 5.0~6.0, and temperature is adjusted to 5 DEG C with 3 DEG C per hour of temperature change, preserve 12h, obtained
To product F；
The temperature of product F is adjusted to 25 DEG C with 3 DEG C per hour of temperature change, then pH to 6.5~7.0 supplements seed
Liquid, the seed liquor of supplement is 5 with the mass ratio of product F:100,12h is preserved, obtain product G；Thermophilic sugar is obtained after product G is air-dried
Gemma bacillus agent；Amino acid organic matter and thermophilic sugared gemma bacillus agent will be contained according to mass ratio 96:After 4 mix, contained
Thermophilic sugared bacillus and the Water soluble fertilizer of amino acid.
Embodiment 3
New fresh sargassum after by clean drying wears into 50~60 mesh powder；
Marine algae powder is with water according to mass ratio 6:100 are fitted into fermentation tank, and sterilize 35min under the conditions of 125 DEG C, is produced
Product A；And prepare seed liquor：Thermophilic sugared bacillus YIC~Alg3 is seeded to fluid nutrient medium culture 24 hours；The thermophilic sugared bud
The preserving number of spore bacillus YIC~Alg3 is CGMCC No12155；The fluid nutrient medium is：Sodium alginate 5g, ammonium sulfate 5g,
The mixture of magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar 20g and distilled water 1000ml, pH7.2~7.4；
The volume ratio of thermophilic sugared bacillus YIC~Alg3 and culture medium is 8:100.Seed liquor is added in product A, wherein seed
Liquid is 4 with the mass ratio of product A:100, obtain product B；It is 30 DEG C in temperature, product B is sent out under conditions of pH7~7.5
Ferment, fermentation time is 9 days, obtains products C；The pH of products C is adjusted to 5.0~6.0, and is adjusted with 1 DEG C per hour of temperature change
Whole temperature preserves 18h to 5 DEG C, obtains product D；With the temperature of 8 DEG C per hour of temperature change adjustment product D to 30 DEG C, pH is extremely
7~7.5, seed liquor is then supplemented, the seed liquor of supplement is 4 with the mass ratio of product D:100,18h is preserved, obtain product E；Adjust
The pH of whole product E adjusts temperature to 5 DEG C to 5.0~6.0 with 1 DEG C per hour of temperature change, preserves 18h, is produced
Product F；
The temperature of product F is adjusted to 30 DEG C with 8 DEG C per hour of temperature change, then pH to 7.0~7.5 supplements seed
Liquid, the seed liquor of supplement is 4 with the mass ratio of product F:100,18h is preserved, obtain product G；Thermophilic sugar is obtained after product G is air-dried
Gemma bacillus agent；Amino acid organic matter and thermophilic sugared gemma bacillus agent will be contained according to mass ratio 96:After 4 mix, contained
Thermophilic sugared bacillus and the Water soluble fertilizer of amino acid.
Embodiment 4
New fresh sargassum after by clean drying wears into 50~60 mesh powder；
Marine algae powder is with water according to mass ratio 9:100 are fitted into fermentation tank, and sterilize 15min under the conditions of 140 DEG C, is produced
Product A；And prepare seed liquor：Thermophilic sugared bacillus YIC~Alg3 is seeded to fluid nutrient medium culture 20 hours；The thermophilic sugared bud
The preserving number of spore bacillus YIC~Alg3 is CGMCC No12155；The fluid nutrient medium is：Sodium alginate 5g, ammonium sulfate 5g,
The mixture of magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar 20g and distilled water 1000ml, pH7.2~7.4；
The volume ratio of thermophilic sugared bacillus YIC~Alg3 and culture medium is 5:100.Seed liquor is added in product A, wherein seed
Liquid is 8 with the mass ratio of product A:100, obtain product B；It is 35 DEG C in temperature, product B is carried out under conditions of pH6.5~7.0
Fermentation, fermentation time obtained products C for 9 days；The pH of products C is adjusted to 5.0~6.0, and is adjusted with 3 DEG C per hour of temperature change
Whole temperature preserves 12h to 5 DEG C, obtains product D；With the temperature of 3 DEG C per hour of temperature change adjustment product D to 35 DEG C, pH is extremely
6.5~7.0, seed liquor is then supplemented, the seed liquor of supplement is 8 with the mass ratio of product D:100,12h is preserved, obtain product E；
The pH of product E is adjusted to 5.0~6.0, and temperature is adjusted to 5 DEG C with 3 DEG C per hour of temperature change, preserve 12h, obtained
Product F；
The temperature of product F is adjusted to 35 DEG C with 3 DEG C per hour of temperature change, then pH to 6.5~7.0 supplements seed
Liquid, the seed liquor of supplement is 8 with the mass ratio of product F:100,12h is preserved, obtain product G；Thermophilic sugar is obtained after product G is air-dried
Gemma bacillus agent；Amino acid organic matter and thermophilic sugared gemma bacillus agent will be contained according to mass ratio 96:After 4 mix, contained
Thermophilic sugared bacillus and the Water soluble fertilizer of amino acid.
In embodiment 1 to 4, the organic matter containing amino acid is sodium glutamate and potassium fulvate mass ratio 17:79 combination
Thing.
Comparative example 1
The temperature and pH value in fermentation process are not adjusted, keep the original temperature of environment and material pH value in itself, other
Condition and material proportion it is consistent with embodiment 2, operating procedure is with embodiment 2.
Comparative example 2
Obstructed everfermentation, without microbial inoculum, with 4 parts of marine algae extracts, 17 parts of sodium glutamate and 79 parts of potassium fulvate
Compound to obtain composition.Described marine algae extract is obstructed everfermentation technique, is extracted from marine alga by way of soaking dissolving
Soluble organic fraction.
Product checking：According to People's Republic of China (PRC) agricultural industry criteria NY/T 798-2015, People's Republic of China's agriculture
Industry professional standard NY/T 1429-2010 detect that testing result is shown in Table 1 to product indices.
Table 1：The quality measurements of the embodiment of the present invention and comparative example
Note：Measure the thermophilic sugared bacillus that the effective viable bacteria in soil refers to present invention use.
Result above shows that the microbial manure indices of present invention production are obviously higher than national standard, Er Qiejing
Everfermentation treatment, the content of heavy metal is substantially reduced in fertilizer, and miscellaneous bacteria rate is substantially reduced, the fermentation work of the use that the present invention is used
Skill can greatly improve the living bacteria count in product, with the obvious advantage in like product.
Embodiments of the invention and comparative example related fertilizer are imposed on into tomato planting plot, mu consumption is 40kg.Planting season
It it is 1 year, soil property specific performance index is shown in Table 2.
Table 2：Property indices after soil improvement
Note：Measure the thermophilic sugared bacillus that the effective viable bacteria in soil refers to present invention use.
Result shows：The present invention use strain survive and grows in soil, soil function be improved significantly, ventilate
Porosity is 13.9%-15.2%, soil pH 6.2-6.4, soil weight 1.38-1.44g/cm3, content of beary metal effectively drops
It is low.
Influence situation using embodiment in the present invention and comparative example product to tomato crop, with the bacterium for applying market purchase
Fertile class product contrast, fertilizer efficiency the results are shown in Table 4.
Table 4：Fertilizer efficiency comparing result
Result shows：In the case where dose is suitable, microbiological compound fertilizer product of the invention is used, hence it is evident that lifting is made
Article matter, greatly reduces effect pest and disease damage, effectively the resistance of enhancing crop, can raising effect yield 9.2%-9.7%.
The preserving number of thermophilic sugared bacillus YIC~Alg3 of the present invention is CGMCC No12155, depositary institution's title：
China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution address：BeiChen West Road, Chaoyang District, BeiJing City 1
Number institute 3, preservation date：02 day 03 month, Latin Classification And Nomenclature in 2016：Bacillus halosaccharovorans.
The biological nature of the thermophilic sugared bacillus YIC~Alg3 is as follows：
After YIC~Alg3 bacterial strains grow 48h on alginic acid sole carbon source flat board, bacterium colony is irregular, faint yellow, and centre is not
Transparent, edge is translucent, surface folding.Micro- sem observation bacterial strain is shaft-like, and gemma, gram can be produced in end or proximal end
The positive, can move, and Later growth thalline is easily assembled agglomerating (Fig. 1).20~45 DEG C of growth temperature range, PH scopes 6~9, NaCl
Concentration range 0~15%, 37 DEG C of bacterial strain optimum growth temperature, the most suitable growth pH 7.5.Catalase, oxidizing ferment, VP experiments are positive, energy
Enough utilize glucose, mannose, mannitol, fructose, lactose, galactolipin, starch；Can not utilize sorbierite, cellulose,.Bacterial strain
16SrDNA sequences see appendix 1, and phylogenetic tree is as shown in Figure 2.According to its physiological and biochemical property, 16SrDNA sequences and system are sent out
Tree information is educated, is thermophilic sugared bacillus halosaccharovorans by the identification of strains.
Note：+ represent and can utilize ,~represent and can not utilize.
Claims (6)
1. it is a kind of to contain thermophilic sugared bacillus（Latin Classification And Nomenclature：Bacillus halosaccharovorans）And amino
The production method of the Water soluble fertilizer of acid, it is characterised in that follow the steps below：
Amino acid organic matter and thermophilic sugared gemma bacillus agent will be contained according to mass ratio 40~99:After 1~60 mixes, obtain containing thermophilic
Sugared bacillus and the Water soluble fertilizer of amino acid；
Wherein organic matter containing amino acid is to extract the one kind or any in the resultant product after monosodium glutamate, sodium glutamate and potassium fulvate
Ratio it is two or more；
Wherein thermophilic sugared gemma bacillus agent is prepared from according to following steps：
1）, prepare marine algae powder：Marine alga after drying is clayed into power；
2）, prepare product A：Marine algae powder is with water according to mass ratio 1~10:100 are fitted into fermentation tank, in 100~150 DEG C of conditions
15~35min of lower sterilizing, obtains product A；
And prepare seed liquor：Thermophilic sugared bacillus is seeded to fluid nutrient medium culture 20~30 hours；
Volume ratio between thermophilic sugared bacillus and culture medium is 1~10:100；
3）, prepare product B：Seed liquor is added to product B is obtained in product A, wherein seed liquor and the mass ratio of product A is 1
~10:100；
4）, prepare products C：It is 20 DEG C~40 DEG C in temperature, product B is fermented under conditions of pH5~9, fermentation time is 6
~10 days, obtain products C；
5）, prepare product D：The pH to 5.0~6.0 of products C is adjusted, temperature preserves 3h~72h to 3~10 DEG C, obtains product D；
6）, prepare product E:To 20 DEG C~40 DEG C, then pH to 5~9 supplements seed liquor, the kind of supplement to the temperature of adjustment product D
Sub- liquid is 1~10 with the mass ratio of product D:100,3h~72h is preserved, obtain product E；
7）, prepare product F：The pH to 5.0~6.0 of product E is adjusted, temperature preserves 3h~72h to 3~10 DEG C, obtains product F；
8）, prepare product G：The temperature of product F is adjusted to 20 DEG C~40 DEG C, then pH to 5~9 supplements seed liquor, the kind of supplement
Sub- liquid is 1~10 with the mass ratio of product F:100,3h~72h is preserved, obtain product G；
9）, prepare thermophilic sugared gemma bacillus agent：Thermophilic sugared gemma bacillus agent is obtained after product G is air-dried；
Wherein thermophilic sugared bacillus, the referred to as YIC~Alg3 of its depositary institution, its preserving number is CGMCC No12155.
2. the production method of the Water soluble fertilizer of thermophilic sugared bacillus and amino acid is contained as claimed in claim 1, it is characterised in that：
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：5 parts of sodium alginate, 5 parts of ammonium sulfate, magnesium sulfate 1
The mixture of part, 2 parts of dipotassium hydrogen phosphate, 0.01 part of ferrous sulfate, 20 parts of agar and 1000 parts of distilled water.
3. the production method of the Water soluble fertilizer of thermophilic sugared bacillus and amino acid is contained as claimed in claim 1, it is characterised in that
Step 2）The mass ratio of the marine algae powder and water is 4:100；115 DEG C of sterilising temp, sterilization time 25min；Prepare seed liquor
When, thermophilic sugared bacillus is seeded in fluid nutrient medium, is cultivated 22 hours, obtains seed liquor, thermophilic sugared bacillus and culture medium
Volume ratio be 5:100；Step 5）With step 7）In, temperature is 5 DEG C, and the holding time is 12h.
4. the production method of the Water soluble fertilizer of thermophilic sugared bacillus and amino acid is contained as claimed in claim 1, it is characterised in that：
Step 4）, step 6）With step 8）In, temperature is 25 DEG C, and pH is 6.5~7.0；Step 4）Fermentation time is 8 days；Step 6）With
Step 8）Holding time is 12h；Step 6）The mass ratio and step 8 of middle fermentation seed liquid and product D）Middle fermentation seed liquid with
The mass ratio of product F is 5:100.
5. the production method of the Water soluble fertilizer of thermophilic sugared bacillus and amino acid is contained as claimed in claim 1, it is characterised in that：
Step 5）, step 6）Step 7）With step 8）In, adjustment temperature is and adjusts 3 DEG C per hour.
6. the production method of the Water soluble fertilizer of thermophilic sugared bacillus and amino acid is contained as claimed in claim 1, it is characterised in that：
The marine alga refers to sargassum thunbergii, the one kind or arbitrary proportion in Sargassum horneri and sargassum it is two or more.
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Citations (4)
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|---|---|---|---|---|
| CN1640854A (en) * | 2004-01-14 | 2005-07-20 | 郭伟光 | Method for producing seeweed, yellow humic acid microbial fertilizer |
| CN102174430A (en) * | 2010-12-31 | 2011-09-07 | 中国海洋大学生物工程开发有限公司 | Bacillus psychrosaccharolyticus strain and application thereof in complex microorganism seaweed fertilizer |
| CN102924134A (en) * | 2012-11-26 | 2013-02-13 | 日照益康有机农业科技发展有限公司 | Alga microorganism fertilizer agent and preparation method thereof |
| CN104774094A (en) * | 2015-04-01 | 2015-07-15 | 大连理工大学 | Preparation method of compound microbial seaweed fertilizer |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1640854A (en) * | 2004-01-14 | 2005-07-20 | 郭伟光 | Method for producing seeweed, yellow humic acid microbial fertilizer |
| CN102174430A (en) * | 2010-12-31 | 2011-09-07 | 中国海洋大学生物工程开发有限公司 | Bacillus psychrosaccharolyticus strain and application thereof in complex microorganism seaweed fertilizer |
| CN102924134A (en) * | 2012-11-26 | 2013-02-13 | 日照益康有机农业科技发展有限公司 | Alga microorganism fertilizer agent and preparation method thereof |
| CN104774094A (en) * | 2015-04-01 | 2015-07-15 | 大连理工大学 | Preparation method of compound microbial seaweed fertilizer |
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Denomination of invention: Production method of water soluble fertilizer containing glycophilic bacillus and amino acids Effective date of registration: 20201126 Granted publication date: 20170517 Pledgee: SINOAGRI HOLDING Co.,Ltd. Pledgor: WUZHOUFENG AGRICULTURAL SCIENCE & TECHNOLOGY Co.,Ltd. Registration number: Y2020980008490 |