CN106220416A - Containing addicted to sugar gemma bacillus agent and the production method of amino acid whose liquid Water soluble fertilizer - Google Patents
Containing addicted to sugar gemma bacillus agent and the production method of amino acid whose liquid Water soluble fertilizer Download PDFInfo
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The present invention relates to that a kind of Sargassum after drying is clayed into power, and marine algae powder is added to the water according to certain ratio containing addicted to sugar gemma bacillus agent and the production method of amino acid whose liquid Water soluble fertilizer, after loading fermentation tank, sterilizing under uniform temperature, certain time；Prepared by seed liquor: screening obtain a strain can degrade alignic fermentation strain YIC～ALG3 addicted to sugar bacillus cereus, be seeded to fluid medium cultivate certain time；By seed liquor according to certain mass than adding in fermentation tank, ferment under certain condition, after fermentation, adjust fermentation condition, after preserving certain time, again adjust fermentation condition and supplement seed liquor, after adjusting three times, filter pressing obtains addicted to sugar bacillus cereus filtrate, by water, join in stirred tank addicted to sugar bacillus cereus filtrate and Kaglutam, after stirring and dissolving is complete, obtain containing addicted to sugar gemma bacillus agent and amino acid whose liquid Water soluble fertilizer.Sweat of the present invention is stablized controlled, and fermentation period is short, and product efficiency is notable, is suitable to large-scale production.
Description
Technical field
The present invention relates to the production method of a kind of liquid Water soluble fertilizer.
Background technology
Marine alga fertilizer, with natural seaweed as raw material, processes through special biochemical process, extracts and retains marine alga natural activity
Composition, a kind of novel organic fertilizer material that refine concentrates and formed.Marine alga fertilizer has raising crop yield and quality, resists disease pest
Effect that evil and poor environment and improvement soil conserve water and soil, it is applied to agricultural production has the huge market demand with good
Development prospect.But, the quality of marine alga fertilizer to be ensured, it is necessary for getting hold of Sargassum raw material and two crucial rings of extraction process
Joint.
First, the quality of marine alga fertilizer quality depends on raw material used.At present, the raw material used by alga fertilizer manufacturing enterprise
Main based on Brown algae, including yellow tang, Thallus Laminariae (Thallus Eckloniae), Thallus Laminariae (Thallus Eckloniae), Alga Sgrgassi Enerves etc..Other algae such as Fucus Vesiculosus, Entermorpha, ulva, Kappa
Zao Dengyeyou minority enterprise with.The external alga fertilizer enterprise overwhelming majority uses Brown algae to be raw material, the most especially applies with yellow tang
The most extensively such as famous brand names such as Seasol, Maxicrop, practical function effect also indicates that yellow tang is the Sargassum raw material of high-quality.
But, China coast does not has yellow tang to be distributed, and the raw material type that domestic enterprise uses is numerous and diverse, and quality is irregular to differ, and causes Sargassum
Fertilizer product quality is very different, and even some producer is turned on the camouflage of " alga fertilizer ", directly blends alginic acid, fulvic acid, nitrogen phosphorus
Potassium etc. pretend to be alga fertilizer, have impact on alga fertilizer quality, have upset alga fertilizer market.
Secondly, the second factor of decision alga fertilizer quality is extraction process.Different extraction processes is to alga fertilizer activity
The kind of composition and content impact are huge.At present, the extracting method of Sargassum active component mainly has chemical method, Physical and life
Thing method.Chemical method processes alginic cell mainly by acid, alkali and organic solvent, makes cell clear up or endogenous substance solubilising, should
Method is simple to operate, easily realizes, and is also the method for the most most alga fertilizer manufacturing enterprises employing.But, chemistry examination
Agent is sizable to the destruction of the active material ingredients in alginic cell, and the organic reagent remained also is potential to environment
Harm.The physical environmental condition such as the principle of Physical is to control pressure, temperature, utilize mechanical force to make alginic cell crush, interior
Tolerant release.Although the method technique cleaning, environmental friendliness, but the requirement for instrument and equipment is harsh, change in reaction conditions
Acutely, relatively costly, it is difficult to realize large-scale production.Bioanalysis includes enzymic degradation and two kinds of methods of fermentable, and it is main
Wanting principle is that to destroy alginic cell wall construction macromolecular substances of degrading be the small-molecule substance that plant easily absorbs, the party
Method reaction gentleness, product diversification, whole production process safety and environmental protection is pollution-free, is preferable alga fertilizer production method.
The research utilizing Production by Microorganism Fermentation marine alga fertilizer is relatively fewer, and only a few example researchs are that selection is multiple micro-
Biological carry out Sargassum raw material is similar to compost fermentation or aerobic fermentation process, there is sweat poor controllability, and fermentation period is long,
The problems such as target product is indefinite, it is difficult to meet the unification of industrialization production requirements, stable, controlled standard, the quality of product
It is difficult to ensure.
The microbial inoculum quantity in the purity of microbial inoculum and unit mass is mainly included for the evaluation index of agricultural micro organism.Cause
This, the bacterial preparation process as prior art is usually: carry out once under the conditions of the temperature suitably survived in microorganism and pH
Fermentation technology, is equivalent to hot-house culture.
Owing to the acidifying of current soil compaction is serious, outside air temperature is changeable, and the microbial bacterial agent of hot-house culture is directly thrown
Being put in soil, cause mortality because not adapting to severe soil environment, surviving because population cannot be formed on a small quantity even if having
Effect, also cannot play the effect of microbial bacterial agent.
Summary of the invention
The technical problem to be solved is to provide a kind of containing addicted to sugar gemma bacillus agent and amino acid whose liquid
The production method of Water soluble fertilizer, first, use function admirable be used for degrade alignic addicted to sugar bacillus cereus degraded Sargassum, and mould
Intend soil natural ecological environment, make putting into after soil addicted to sugar bacillus cereus after fermentation, can quickly adapt to environment, it is ensured that survival
Rate；The second, provide utilize described addicted to sugar bacillus cereus preparation addicted to sugar bacillus cereus and containing addicted to sugar bacillus cereus containing aminoacid
The method of liquid Water soluble fertilizer, the method sweat is stablized controlled, and fermentation period is short, and product efficiency is notable, is suitable to scale metaplasia
Produce.
In order to solve above-mentioned technical problem, the present invention by the following technical solutions:
A kind of containing addicted to sugar gemma bacillus agent and the production method of amino acid whose liquid Water soluble fertilizer, it is characterised in that according to
Following steps are carried out:
By water, Kaglutam and join stirring addicted to sugar bacillus cereus filtrate according to mass ratio 50～75: 5～40: 1～85
In still, after stirring and dissolving, obtain containing addicted to sugar gemma bacillus agent and amino acid whose liquid Water soluble fertilizer；
Wherein it is prepared from according to following steps addicted to sugar bacillus cereus filtrate:
1), marine algae powder is prepared: the Sargassum after drying is clayed into power；
2), preparation product A: marine algae powder loads in fermentation tank with water according to mass ratio 1～10: 100, at 100～150 DEG C
Under the conditions of sterilizing 15～35min, obtain product A；
And prepare seed liquor: fluid medium will be seeded to addicted to sugar bacillus cereus and cultivate 20～30 hours；
It is 1～10: 100 addicted to the volume ratio between sugar bacillus cereus and culture medium；
3), preparation product B: seed liquor is joined in product A and obtain product B, wherein seed liquor and the mass ratio of product A
It is 1～10: 100；
4), preparation products C: be 20 DEG C～40 DEG C in temperature, under conditions of pH5～9, product B fermented, during fermentation
Between be 6～10 days, obtain products C；
5) product D, is prepared: adjust the pH to 5.0～6.0 of products C, temperature to 3～10 DEG C, preserve 3h～72h, produced
Product D；
6), preparation product E: adjust product D temperature to 20 DEG C～40 DEG C, pH to 5～9, then supplement seed liquor, supplement
The mass ratio of seed liquor and product D be 1～10: 100, preserve 3h～72h, obtain product E；
7), preparation product F: adjust the pH to 5.0～6.0 of product E, temperature to 3～10 DEG C, preserve 3h～72h, produced
Product F；
8), preparation product G: adjust product F temperature to 20 DEG C～40 DEG C, pH to 5～9, then supplement seed liquor, supplement
The mass ratio of seed liquor and product F be 1～10: 100, preserve 3h～72h, obtain product G；
9), preparation is addicted to sugar bacillus cereus filtrate: will obtain after product G filter pressing addicted to sugar bacillus cereus filtrate.
The pH7.2～7.4 of described fluid medium, and be made into according to following mass ratio: sodium alginate 5 parts, ammonium sulfate 5
Part, 1 part of magnesium sulfate, dipotassium hydrogen phosphate 2 parts, 0.01 part of ferrous sulfate, 20 parts of agar and the mixture of distilled water 1000 parts.
Step 2) mass ratio of described marine algae powder and water is 4: 100；Sterilising temp 115 DEG C, sterilization time 25min；System
During standby seed liquor, it is seeded in fluid medium addicted to sugar bacillus cereus, cultivates 22 hours, obtain seed liquor, addicted to sugar bacillus cereus
It is 5: 100 with the volume ratio of culture medium；Step 5) and step 7) in, temperature is 5 DEG C, and the holding time is 12h.
Step 4), step 6) and step 8) in, temperature is 25 DEG C, and pH is 6.5～7.0；Step 4) fermentation time is 8 days；
Step 6) and step 8) holding time is 12h；Step 6) in the mass ratio of fermentation seed liquid and product D and step 8) in fermentation
Seed liquor is 5: 100 with the mass ratio of product F.
Step 5), step 6) step 7) and step 8) in, adjust temperature be per hour adjust 3 DEG C.
Described Sargassum refers to the two or more of a kind of or arbitrary proportion in sargassum thunbergii, Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) and Alga Sgrgassi Enerves.
Described is that its preserving number is CGMCC No.12155 addicted to sugar bacillus cereus YIC～Alg3 addicted to sugar bacillus cereus.
Invent and there is following Advantageous Effects:
The present invention by screening obtain a strain marine source addicted to sugar bacillus cereus, this bacterial strain have degraded alignic energy
Power, alginic acid of on the one hand can degrading generates the brown alga oligose etc. of small-molecular-weight and is prone to the nutritional labeling that plant absorption utilizes；Separately
On the one hand destroy more nutrient substance in alginic cell wall construction promotes Sargassum body to be released.This strain fermentation is utilized to process copper
The Sargassum Sargassum raw material such as algae, sargassum thunbergii, can detect that in fermentation liquid after certain natural law that ferments molecular weight 4000Da with
Under, the degree of polymerization be the brown alga oligose of 2-20, using this fermentation liquid as alga fertilizer mother solution, direct or compounding N, P, K, trace unit
Element, aminoacid, the effective ingredient such as organic is for crop growth.Fertilizer efficiency experiment shows, utilizes the method to obtain fermentation liquid energy
Enough it is obviously promoted plant root growth, improves Soil Micro-environment, improve crop yield.
The present invention also has the following characteristics that
1, use the marine source that obtains of the present invention addicted to sugar bacillus cereus YIC～Alg3 to fermentation of seaweed, sweat
Stablizing controlled, fermentation period is short, and product efficiency is notable, is suitable to large-scale production.
What 2, the present invention obtained has good water solubility addicted to sugar bacillus cereus, it is easy to plant absorption utilizes, simultaneously as one
Important signaling molecule, it is possible to the growth regulating of involved in plant and induction resistance mechanism promote the growth of plant, improve plant pair
Poor environment and the resistance of pest and disease damage.
3, the present invention is regulated by temperature slowly, makes the change addicted to sugar bacillus cereus adaptive temperature, it is to avoid variations in temperature
Too fast, do not adapt to and mortality addicted to sugar bacillus cereus.
What 4, the present invention obtained has outside Sargassum extract feature addicted to sugar bacillus cereus, also has stronger environment and adapts to
Property, in the soil put into acidifying and in the environment of variations in temperature impermanence, can quickly adapt to environment survival, play microbial bacteria
The effect of agent.
Accompanying drawing explanation
Fig. 1 is microexamination YIC～the Gram’s staining of Alg3 bacterial strain and assembles agglomerating phenomenon figure.
Fig. 2 is YIC～Alg3 bacterial strain phylogenetic tree schematic diagram based on 16SrDNA sequence construct.
Detailed description of the invention
The present invention is further illustrated below in conjunction with instantiation.
Embodiment 1
Fresh sargassum thunbergii after clean drying is worn into 50～60 mesh powder；
Marine algae powder loads in fermentation tank according to mass ratio 2: 100 with water, and under the conditions of 115 DEG C, sterilizing 35min, is produced
Product A；And prepare seed liquor: fluid medium will be seeded to addicted to sugar bacillus cereus YIC～Alg3 and cultivate 20 hours；Described addicted to sugar bud
The preserving number of spore bacillus YIC～Alg3 is CGMCC No.12155；Described fluid medium is: sodium alginate 5g, ammonium sulfate 5g,
Magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar 20g and the mixture of distilled water 1000ml, pH7.2～7.4；
Volume ratio addicted to sugar bacillus cereus YIC～Alg3 and culture medium is 2: 100.Seed liquor is joined in product A, wherein seed
Liquid is 1: 100 with the mass ratio of product A, obtains product B；It is 20 DEG C in temperature, under conditions of pH5.5～6.0, product B is carried out
Fermentation, fermentation time is 7 days, obtains products C；Adjust the pH to 5.0～6.0 of products C, and with the variations in temperature of per hour 20 DEG C
Adjustment temperature, to 5 DEG C, preserves 24h, obtains product D；The temperature of product D is adjusted to 20 DEG C with the variations in temperature of per hour 20 DEG C,
PH to 5.5～6.0, then supplements seed liquor, and supplementary seed liquor is 1: 100 with the mass ratio of product D, preserves 24h, is produced
Product E；Adjust the pH to 5.0～6.0 of product E, and adjust temperature to 5 DEG C with the variations in temperature of per hour 20 DEG C, preserve
24h, obtains product F；
The temperature of product F is adjusted to 20 DEG C with the variations in temperature of per hour 20 DEG C, pH to 5.5～6.0, then supplements seed
Liquid, supplementary seed liquor is 1: 100 with the mass ratio of product F, preserves 24h, obtains product G；By obtain after product G filter pressing addicted to
Sugar bacillus cereus YIC～Alg3 filtrate；By water, Kaglutam and addicted to sugar bacillus cereus YIC～Alg3 filtrate according to mass ratio 70:
After join and dissolve completely in stirred tank at 22: 8, obtain containing addicted to sugared gemma bacillus agent and amino acid whose liquid Water soluble fertilizer.
Embodiment 2
Fresh Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) after clean drying and new fresh sargassum are compounded according to mass ratio 1: 1, wears into 50～60 mesh powder；
Marine algae powder loads in fermentation tank according to mass ratio 4: 100 with water, and under the conditions of 115 DEG C, sterilizing 25min, is produced
Product A；And prepare seed liquor: fluid medium will be seeded to addicted to sugar bacillus cereus YIC～Alg3 and cultivate 22 hours；Described addicted to sugar bud
The preserving number of spore bacillus YIC～Alg3 is CGMCC No.12155；Described fluid medium is: sodium alginate 5g, ammonium sulfate 5g,
Magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar 20g and the mixture of distilled water 1000ml, pH7.2～7.4；
Volume ratio addicted to sugar bacillus cereus YIC～Alg3 and culture medium is 5: 100.Seed liquor is joined in product A, wherein seed
Liquid is 5: 100 with the mass ratio of product A, obtains product B；It is 25 DEG C in temperature, under conditions of pH6.5～7.0, product B is carried out
Fermentation, fermentation time is 8 days, obtains products C；Adjust the pH to 5.0～6.0 of products C, and with the variations in temperature of per hour 3 DEG C
Adjustment temperature, to 5 DEG C, preserves 12h, obtains product D；The temperature of product D is adjusted to 25 DEG C, pH with the variations in temperature of per hour 3 DEG C
To 6.5～7.0, then supplementing seed liquor, supplementary seed liquor is 5: 100 with the mass ratio of product D, preserves 12h, obtains product
E；Adjust the pH to 5.0～6.0 of product E, and adjust temperature to 5 DEG C with the variations in temperature of per hour 3 DEG C, preserve 12h,
To product F；The temperature of product F is adjusted to 25 DEG C with the variations in temperature of per hour 3 DEG C, pH to 6.5～7.0, then supplements seed
Liquid, supplementary seed liquor is 5: 100 with the mass ratio of product F, preserves 12h, obtains product G；Obtain addicted to sugar after product G filter pressing
Bacillus cereus YIC～Alg3 filtrate；By water, Kaglutam and addicted to sugar bacillus cereus YIC～Alg3 filtrate according to mass ratio 70: 22
: after 8 join and dissolve completely in stirred tank, obtain containing addicted to sugared gemma bacillus agent and amino acid whose liquid Water soluble fertilizer.
Embodiment 3
New fresh sargassum after clean drying is worn into 50～60 mesh powder；
Marine algae powder loads in fermentation tank according to mass ratio 6: 100 with water, and under the conditions of 125 DEG C, sterilizing 35min, is produced
Product A；And prepare seed liquor: fluid medium will be seeded to addicted to sugar bacillus cereus YIC～Alg3 and cultivate 24 hours；Described addicted to sugar bud
The preserving number of spore bacillus YIC～Alg3 is CGMCC No.12155；Described fluid medium is: sodium alginate 5g, ammonium sulfate 5g,
Magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar 20g and the mixture of distilled water 1000ml, pH7.2～7.4；
Volume ratio addicted to sugar bacillus cereus YIC～Alg3 and culture medium is 8: 100.Seed liquor is joined in product A, wherein seed
Liquid is 4: 100 with the mass ratio of product A, obtains product B；It is 30 DEG C in temperature, carries out product B under conditions of pH7～7.5 sending out
Ferment, fermentation time is 9 days, obtains products C；Adjust the pH to 5.0～6.0 of products C, and adjust with the variations in temperature of per hour 1 DEG C
Whole temperature, to 5 DEG C, preserves 18h, obtains product D；Adjusting the temperature of product D to 30 DEG C with the variations in temperature of per hour 8 DEG C, pH is extremely
7～7.5, then supplement seed liquor, supplementary seed liquor is 4: 100 with the mass ratio of product D, preserves 18h, obtains product E；
Adjust the pH to 5.0～6.0 of product E, and adjust temperature to 5 DEG C with the variations in temperature of per hour 1 DEG C, preserve 18h, obtain
Product F；
The temperature of product F is adjusted to 30 DEG C with the variations in temperature of per hour 8 DEG C, pH to 7.0～7.5, then supplements seed
Liquid, supplementary seed liquor is 4: 100 with the mass ratio of product F, preserves 18h, obtains product G；Obtain addicted to sugar after product G filter pressing
Bacillus cereus YIC～Alg3 filtrate；By water, Kaglutam and addicted to sugar bacillus cereus YIC～Alg3 filtrate according to mass ratio 70: 22
: after 8 join and dissolve completely in stirred tank, obtain containing addicted to sugared gemma bacillus agent and amino acid whose liquid Water soluble fertilizer.
Embodiment 4
New fresh sargassum after clean drying is worn into 50～60 mesh powder；
Marine algae powder loads in fermentation tank according to mass ratio 9: 100 with water, and under the conditions of 140 DEG C, sterilizing 15min, is produced
Product A；And prepare seed liquor: fluid medium will be seeded to addicted to sugar bacillus cereus YIC～Alg3 and cultivate 20 hours；Described addicted to sugar bud
The preserving number of spore bacillus YIC～Alg3 is CGMCC No.12155；Described fluid medium is: sodium alginate 5g, ammonium sulfate 5g,
Magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar 20g and the mixture of distilled water 1000ml, pH7.2～7.4；
Volume ratio addicted to sugar bacillus cereus YIC～Alg3 and culture medium is 5: 100.Seed liquor is joined in product A, wherein seed
Liquid is 8: 100 with the mass ratio of product A, obtains product B；It is 35 DEG C in temperature, under conditions of pH6.5～7.0, product B is carried out
Fermentation, fermentation time is to obtain products C in 9 days；Adjust the pH to 5.0～6.0 of products C, and adjust with the variations in temperature of per hour 3 DEG C
Whole temperature, to 5 DEG C, preserves 12h, obtains product D；Adjusting the temperature of product D to 35 DEG C with the variations in temperature of per hour 3 DEG C, pH is extremely
6.5～7.0, then supplement seed liquor, supplementary seed liquor is 8: 100 with the mass ratio of product D, preserves 12h, obtains product E；
Adjust the pH to 5.0～6.0 of product E, and adjust temperature to 5 DEG C with the variations in temperature of per hour 3 DEG C, preserve 12h, obtain
Product F；
The temperature of product F is adjusted to 35 DEG C with the variations in temperature of per hour 3 DEG C, pH to 6.5～7.0, then supplements seed
Liquid, supplementary seed liquor is 8: 100 with the mass ratio of product F, preserves 12h, obtains product G；Obtain addicted to sugar after product G filter pressing
Bacillus cereus YIC～Alg3 filtrate；By water, Kaglutam and addicted to sugar bacillus cereus YIC～Alg3 filtrate according to mass ratio 70: 22
: after 8 join and dissolve completely in stirred tank, obtain containing addicted to sugared gemma bacillus agent and amino acid whose liquid Water soluble fertilizer.
Comparative example 1
Do not regulate the temperature in sweat and pH value, keep the original temperature of environment and the pH value of material itself, other
Condition and material proportion consistent with embodiment 2, operating procedure is with embodiment 2.
Comparative example 2
Obstructed everfermentation, without microbial inoculum, substitutes microbial bacterial agent with Sargassum extract, by water, Kaglutam and Sargassum
Extract joins according to mass ratio 70: 22: 8 after dissolving completely in stirred tank, obtains amino acid whose liquid alga Water soluble fertilizer.Institute
The Sargassum extract stated is obstructed everfermentation technique, the organic one-tenth of solubility extracted from Sargassum by the way of immersion is dissolved
Point.
Product checking: according to People's Republic of China (PRC) agricultural industry criteria NY/T 798-2015, People's Republic of China's agriculture
Product indices is detected by industry industry standard NY/T 1429-2010, and testing result is shown in Table 1.
Table 1: the embodiment of the present invention and the quality measurements of comparative example
Note: effectively viable bacteria refer to that the present invention uses addicted to sugar bacillus cereus.
Result above shows, the microbial manure indices that the present invention produces is obviously higher than national standard, Er Qiejing
Everfermentation processes, and in fertilizer, the content of heavy metal substantially reduces, and miscellaneous bacteria rate substantially reduces, the fermentation work of the employing that the present invention uses
Skill can be greatly improved the living bacteria count in product, with the obvious advantage in like product.
Embodiments of the invention and comparative example related fertilizer are imposed on tomato planting plot, and mu consumption is 40kg.Planting season
Being 1 year, soil property specific performance index is shown in Table 2.
Table 2: the property indices after soil improvement
Note: effectively viable bacteria refer to that the present invention uses addicted to sugar bacillus cereus.
Result shows: the present invention use strain survive in soil and grow, soil function be improved significantly, ventilate
Porosity is 13.0%-14.6%, soil pH 6.3-6.5, soil weight 1.36-1.38g/cm3, content of beary metal effectively drops
Low.
Preserving number addicted to sugar bacillus cereus YIC～Alg3 of the present invention is CGMCC No.12155, depositary institution's title:
China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution address: BeiChen West Road, Chaoyang District, BeiJing City 1
Number institute 3, preservation date: on 03 02nd, 2016, Latin Classification And Nomenclature: Bacillus halosaccharovorans.
The described biological nature addicted to sugar bacillus cereus YIC～Alg3 is as follows:
After YIC～Alg3 bacterial strain grows 48h on alginic acid sole carbon source flat board, bacterium colony is irregular, and faint yellow, centre is not
Transparent, edge is translucent, surface folding.It is shaft-like that microscope observes bacterial strain, can produce spore, gram at end or proximal end
The positive, can move, and Later growth thalline easily assembles agglomerating (Fig. 1).Growth temperature range 20～45 DEG C, PH scope 6～9, NaCl
Concentration range 0～15%, bacterial strain optimum growth temperature 37 DEG C, the most suitable growth pH 7.5.Catalase, oxidase, VP tests the positive, energy
Enough utilize glucose, mannose, mannitol, fructose, lactose, galactose, starch；Can not utilize sorbitol, cellulose,.Bacterial strain
16SrDNA sequence sees appendix 1, and phylogenetic tree is as shown in Figure 2.According to its physiological and biochemical property, 16SrDNA sequence and system are sent out
Educate tree information, be addicted to sugar bacillus halosaccharovorans by this identification of strains.
Note :+represent and can utilize ,～represent and can not utilize.
Claims (7)
1. one kind containing addicted to sugar gemma bacillus agent and the production method of amino acid whose liquid Water soluble fertilizer, it is characterised in that according to
Lower step is carried out:
By water, Kaglutam and join stirred tank addicted to sugar bacillus cereus filtrate according to mass ratio 50～75:5～40:1～85
In, after stirring and dissolving, obtain containing addicted to sugar gemma bacillus agent and amino acid whose liquid Water soluble fertilizer；
Wherein it is prepared from according to following steps addicted to sugar bacillus cereus filtrate:
1), marine algae powder is prepared: the Sargassum after drying is clayed into power；
2), preparation product A: marine algae powder loads in fermentation tank with water according to mass ratio 1～10:100,100～150 DEG C of conditions
Lower sterilizing 15～35min, obtains product A；
And prepare seed liquor: fluid medium will be seeded to addicted to sugar bacillus cereus and cultivate 20～30 hours；
It is 1～10:100 addicted to the volume ratio between sugar bacillus cereus and culture medium；
3), preparation product B: seed liquor being joined in product A and obtain product B, wherein seed liquor is 1 with the mass ratio of product A
～10:100；
4), preparation products C: being 20 DEG C～40 DEG C in temperature, under conditions of pH5～9 ferment product B, fermentation time is 6
～10 days, obtain products C；
5) product D, is prepared: adjust the pH to 5.0～6.0 of products C, temperature to 3～10 DEG C, preserve 3h～72h, obtain product D；
6), preparation product E: adjust product D temperature to 20 DEG C～40 DEG C, pH to 5～9, then supplement seed liquor, supplementary kind
Sub-liquid is 1～10:100 with the mass ratio of product D, preserves 3h～72h, obtains product E；
7), preparation product F: adjust the pH to 5.0～6.0 of product E, temperature to 3～10 DEG C, preserve 3h～72h, obtain product F；
8), preparation product G: adjust product F temperature to 20 DEG C～40 DEG C, pH to 5～9, then supplement seed liquor, supplementary kind
Sub-liquid is 1～10:100 with the mass ratio of product F, preserves 3h～72h, obtains product G；
9), preparation is addicted to sugar bacillus cereus filtrate: will obtain after product G filter pressing addicted to sugar bacillus cereus filtrate.
2. contain as claimed in claim 1 addicted to sugar gemma bacillus agent and the production method of amino acid whose liquid Water soluble fertilizer, its
It is characterised by: the pH7.2～7.4 of described fluid medium, and is made into according to following mass ratio: sodium alginate 5 parts, ammonium sulfate 5
Part, 1 part of magnesium sulfate, dipotassium hydrogen phosphate 2 parts, 0.01 part of ferrous sulfate, 20 parts of agar and the mixture of distilled water 1000 parts.
3. contain as claimed in claim 1 addicted to sugar gemma bacillus agent and the production method of amino acid whose liquid Water soluble fertilizer, its
It being characterised by step 2) mass ratio of described marine algae powder and water is 4:100；Sterilising temp 115 DEG C, sterilization time 25min；System
During standby seed liquor, it is seeded in fluid medium addicted to sugar bacillus cereus, cultivates 22 hours, obtain seed liquor, addicted to sugar bacillus cereus
It is 5:100 with the volume ratio of culture medium；In step 5) and step 7), temperature is 5 DEG C, and the holding time is 12h.
4. contain as claimed in claim 1 addicted to sugar gemma bacillus agent and the production method of amino acid whose liquid Water soluble fertilizer, its
Being characterised by: in step 4), step 6) and step 8), temperature is 25 DEG C, and pH is 6.5～7.0；Step 4) fermentation time is 8 days；
Step 6) and step 8) holding time are 12h；Step 6) is fermented in the mass ratio of fermentation seed liquid and product D and step 8)
Seed liquor is 5:100 with the mass ratio of product F.
5. contain as claimed in claim 1 addicted to sugar gemma bacillus agent and the production method of amino acid whose liquid Water soluble fertilizer, its
It is characterised by: in step 5), step 6) step 7) and step 8), adjusts temperature and be adjustment 3 DEG C per hour.
6. contain as claimed in claim 1 addicted to sugar gemma bacillus agent and the production method of amino acid whose liquid Water soluble fertilizer, its
It is characterised by: described Sargassum refers to the two or more of a kind of or arbitrary proportion in sargassum thunbergii, Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) and Alga Sgrgassi Enerves.
7. the production method containing the Water soluble fertilizer addicted to sugar bacillus cereus and humic acids as claimed in claim 1, it is characterised in that:
Described is that its preserving number is CGMCC No.12155 addicted to sugar bacillus cereus YIC～Alg3 addicted to sugar bacillus cereus.
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