CN106396907A - Production method of water-soluble fertilizer containing Bacillusmegaterium fungicide and amino acids - Google Patents
Production method of water-soluble fertilizer containing Bacillusmegaterium fungicide and amino acids Download PDFInfo
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- CN106396907A CN106396907A CN201610770117.7A CN201610770117A CN106396907A CN 106396907 A CN106396907 A CN 106396907A CN 201610770117 A CN201610770117 A CN 201610770117A CN 106396907 A CN106396907 A CN 106396907A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention relates to a production method of a water-soluble fertilizer containing Bacillusmegaterium fungicide and amino acids. According to the method, firstly, the dried seaweed is ground into powders, and seaweed powders are added into the water according to a certain ratio. After that, an obtained mixture is fed into a fermentation tank and sterilized at a certain temperature for a certain period of time. Secondly, a seed solution is prepared, wherein a fermentation strain of bacillus megaterium YIC-BM1 capable of generating cellulase is screened out, inoculated into a liquid culture medium and cultured for a certain period of time. The obtained seed solution is added into the fermentation tank according to a certain mass ratio, and fermented under a certain condition. After the fermentation process, the fermentation condition is adjusted and maintained for a certain period of time. After that, the fermentation condition is adjusted again and then the seed solution is replenished. After the fermentation condition is adjusted three times, the fermentation broth is air-dried to obtain the Bacillusmegaterium fungicide. The Bacillusmegaterium fungicide and amino acid-containing organic substances are mixed up and uniformly stirred to prepare the water-soluble fertilizer containing Bacillusmegaterium fungicide and amino acids. The fermentation process is stable and controllable, and the fermentation period is short. Meanwhile, obtained products are obvious in effect, and suitable for large-scale production.
Description
Technical field
The present invention relates to a kind of production method containing bacillus megaterium and the Water soluble fertilizer of amino acid.
Background technology
Marine alga fertilizer, with natural seaweed as raw material, is processed through special biochemical process, extracts and retain marine alga natural activity
Composition, a kind of novel organic fertilizer material that refining concentrates and formed.Marine alga fertilizer has raising crop yield and quality, resists disease pest
Effect that evil and poor environment and improved soil conserve water and soil, it is applied to agricultural production the huge market demand and good
Development prospect.But, the quality of marine alga fertilizer to be ensured, it is necessary for getting hold of marine alga raw material and the crucial ring of extraction process two
Section.
First, the quality of marine alga fertilizer quality depends on raw material used.At present, the raw material used by alga fertilizer manufacturing enterprise
Mainly based on brown alga, including yellow tang, kelp, sea-tangle, sargassum etc..Other algae such as bladder-wrack, Enteromorpha, sea lettuce, Kappa
Zao Dengyeyou minority enterprise with.External alga fertilizer enterprise is most to be raw material using brown alga, is wherein especially applied with yellow tang
The most extensive such as famous brand name such as Seasol, Maxicrop, practical function effect also indicates that yellow tang is the marine alga raw material of high-quality.
But, China coast does not have yellow tang to be distributed, and the raw material type that domestic enterprise adopts is numerous and diverse, and quality is irregular to differ, and causes marine alga
Fertilizer product quality is very different, or even some producers are turned on the camouflage of " alga fertilizer ", directly blends alginic acid, fulvic acid, nitrogen phosphorus
Potassium etc. pretends to be alga fertilizer, have impact on alga fertilizer quality, has upset alga fertilizer market.
Secondly, the second factor of decision alga fertilizer quality is extraction process.Different extraction processes is to alga fertilizer activity
The species and content impact of composition is huge.At present, the extracting method of marine alga active component mainly has chemical method, Physical and life
Thing method.Chemical method mainly uses acid, alkali and organic solvent and processes alginic cell, so that cell is cleared up or endogenous substance solubilising, should
Method is simple to operate, easily realizes, and is also the method that most alga fertilizer manufacturing enterprises adopt both at home and abroad.But, chemistry examination
Agent is sizable to the destruction of the active material ingredients in alginic cell, and the organic reagent remaining is also potential to environment
Harm.The principle of Physical is control pressure, the physical environmental condition such as temperature, so that alginic cell is crushed using mechanical force, interior
Tolerant release.Although the method technique cleaning, environmental friendliness, the requirement for instrument and equipment is harsh, change in reaction conditions
Acutely, relatively costly it is difficult to realize large-scale production.Bioanalysis includes two methods of enzymic degradation and fermentable, its master
Principle is wanted to be to destroy the small-molecule substance that alginic cell wall construction macromolecular substances of degrading easily absorb, the party for plant
Method reaction is gentle, product diversification, and whole production process safety and environmental protection is pollution-free, is preferable marine alga fertilizer production method.
Research using Production by Microorganism Fermentation marine alga fertilizer is relatively fewer, and only several researchs are from multiple micro-
Biology carries out similar compost fermentation to marine alga raw material or aerobic fermentation is processed, and there is sweat poor controllability, fermentation period is long,
The problems such as target product is indefinite is it is difficult to meet the unification of industrialization production requirements, stable, controlled standard, the quality of product
It is difficult to ensure.
Evaluation index for agricultural micro organism mainly includes the microbial inoculum quantity in the purity and unit mass of microbial inoculum.Cause
This, the bacterial preparation process as prior art is usually：Carry out once under the conditions of the temperature of the suitable existence of microorganism and pH
Zymotechnique, is equivalent to hot-house culture.
Because the acidifying of current soil compaction is serious, outside air temperature is changeable, and the microbial bacterial agent of hot-house culture is directly thrown
It is put in soil, the soil environment because not adapting to severe causes mortality, even if having surviving because population cannot be formed on a small quantity
Effect, also cannot play the effect of microbial bacterial agent.
Content of the invention
The technical problem to be solved is to provide a kind of water-soluble containing bacillus megaterium microbial inoculum and amino acid
Fertile production method, first, using function admirable cellulase-producing bacillus megaterium degraded marine alga, and simulate soil from
So ecological environment, makes the bacillus megaterium after fermentation after putting into soil, can adapt to environment quickly it is ensured that survival rate；Secondth,
There is provided and prepare bacillus megaterium and containing bacillus megaterium containing amino acid Water soluble fertilizer using described bacillus megaterium
Method, the method sweat is stablized controlled, and fermentation period is short, and product efficiency is notable, is suitable to large-scale production.
In order to solve above-mentioned technical problem, the present invention employs the following technical solutions：
A kind of production method containing bacillus megaterium microbial inoculum and the Water soluble fertilizer of amino acid is it is characterised in that according to following steps
Carry out：
Amino acid organic matter and bacillus megaterium microbial inoculum will be contained according to mass ratio 40～99:After 1～60 mixes, obtain containing huge
Bacterium anthracoides and the Water soluble fertilizer of amino acid；
One of resultant product after wherein organic matter containing amino acid is extraction monosodium glutamate, sodium glutamate and potassium fulvate or any
Ratio two or more；
Wherein bacillus megaterium microbial inoculum is prepared from according to following steps：
1）, prepare marine algae powder：Marine alga after drying is clayed into power；
2）, prepare product A：Marine algae powder and water are according to mass ratio 1～10:100 load in fermentation tank, in 100～150 DEG C of conditions
Lower sterilizing 15～35min, obtains product A；
And prepare seed liquor：Bacillus megaterium is seeded to fluid nutrient medium, cultivates 20～30 hours at 25 DEG C～40 DEG C；Huge
Volume ratio between Bacterium anthracoides and culture medium is 1～10:100；
3）, prepare product B：Seed liquor is added in product A and obtains product B, wherein seed liquor and the mass ratio of product A are 1
～10:100；
4）, preparation products C：It is 25 DEG C～40 DEG C in temperature, under conditions of pH5～9, product B is fermented, fermentation time is 6
～10 days, obtain products C；
5）, prepare product D：The pH to 5.0～6.0 of adjustment products C, temperature, to 3～10 DEG C, preserves 3h～72h, obtains product D；
6）, preparation product E:Adjustment product D temperature to 25 DEG C～40 DEG C, pH to 5～9, then supplement seed liquor, supplement kind
Sub- liquid is 1～10 with the mass ratio of product D:100, preserve 3h～72h, obtain product E；
7）, preparation product F：The pH to 5.0～6.0 of adjustment product E, temperature, to 3～10 DEG C, preserves 3h～72h, obtains product F；
8）, preparation product G：Adjustment product F temperature to 25 DEG C～40 DEG C, pH to 5～9, then supplement seed liquor, supplement kind
Sub- liquid is 1～10 with the mass ratio of product F:100, preserve 3h～72h, obtain product G；
9）, prepare the bacillus megaterium YIC～BM1 microbial inoculum of cellulase-producing：Cellulase-producing is obtained after product G is air-dried
Bacillus megaterium microbial inoculum；
Described fluid nutrient medium is made into according to following mass ratio：5 parts of sodium alginate, 5 parts of ammonium sulfate, 1 part of magnesium sulfate, phosphoric acid hydrogen two
2 parts of potassium, 0.01 part of ferrous sulfate, 20 parts of agar and 1000 parts of distilled water.
Step 2）The mass ratio of described marine algae powder and water is 4:100；115 DEG C of sterilising temp, sterilization time 25min；System
During standby seed liquor, bacillus megaterium is seeded in fluid nutrient medium, cultivates 22 hours, obtains seed liquor, bacillus megaterium
Volume ratio with culture medium is 5:100；Step 5）With step 7）In, temperature is 5 DEG C, and the holding time is 12h.
Step 4）, step 6）With step 8）In, temperature is 25 DEG C, and pH is 6.5～7.0；Step 4）Fermentation time is 8 days；
Step 6）With step 8）Holding time is 12h；Step 6）The mass ratio of middle fermentation seed liquid and product D and step 8）Middle fermentation
Seed liquor is 5 with the mass ratio of product F:100.
Step 5）, step 6）Step 7）With step 8）In, adjustment temperature is and adjusts 3 DEG C per hour.
Described organic matter containing amino acid is single amino acid, the salt of single amino acid, compound amino acid, compound ammonia
The salt of base acid, lysine factory and one of Gourmet Powder Factory's leftover bits and pieces, molasses fermented liquid and yeast factory leftover bits and pieces etc. or any
Two kinds of ratio and more than.
Mass ratio between organic matter containing amino acid and bacillus megaterium microbial inoculum is 96:4.
Described marine alga refers to the two or more of sargassum thunbergii, one of Sargassum horneri and sargassum or arbitrary proportion.
Described bacillus megaterium is bacillus megaterium YIC～BM1, and preserving number is GMCC No.12156.
Invention has following Advantageous Effects：
The bacillus megaterium that the present invention adopts has the ability of cellulase-producing, and alginic acid of on the one hand can degrading generates little point
Brown alga oligose of son amount etc. is easy to the nutrient content of plant absorption utilization；On the other hand destroy alginic cell wall construction and promote marine alga
More nutriments are released in vivo.Process the Sargassum marine alga raw materials such as Sargassum horneri, sargassum thunbergii, fermentation using this strain fermentation
Air-dried with this zymotic fluid after certain number of days and make pulvis, direct or compounding N, P, K, trace element, amino acid, organic matter etc. has
Effect composition is used for crop growth.Fertilizer efficiency experiment shows, obtains zymotic fluid using the method and can be obviously promoted root system of plant life
Long, improved soil microenvironment, improves crop yield.
The present invention also has the characteristics that：
1st, the bacillus megaterium YIC～BM1 of the marine source being obtained using the present invention, to fermentation of seaweed, can destroy marine alga thin
Cell wall structures promote more nutriments in marine alga body to be released, and sweat is stablized controlled, and fermentation period is short, product efficiency
Significantly, it is suitable to large-scale production.
2nd, amino acid is combined by the present invention with active ingredient in marine alga, can significantly improve that crop is degeneration-resistant, disease resistance.It is easy to
Plant absorption utilizes, and simultaneously as a kind of important signaling molecule, is capable of growth regulating and the induction resistance mechanism of involved in plant
Promote the growth of plant, improve the resistance to poor environment and pest and disease damage for the plant.
3rd, the present invention makes the bacterium that fermentation goes out possess the ability adapting to severe soil environment by adjusting fermentation condition, favorably
Performance in bacterium function.
4th, the present invention, by slow temperature adjustment, makes the change of the bacillus megaterium adaptive temperature of cellulase-producing,
Avoid temperature change too fast, the bacillus megaterium of cellulase-producing does not adapt to and mortality.
Brief description
Fig. 1 is the Gram's staining of microexamination YIC～BM1 bacterial strain and assembles agglomerating phenomenon figure.
YIC～BM1 bacterial strain phylogenetic tree schematic diagram based on 16SrDNA sequence construct for the Fig. 2.
Fig. 3 is the schematic diagram of the impact to YIC～BM1 strain enzyme-producing for the different temperatures.
Fig. 4 is the impact schematic diagram to YIC～BM1 strain enzyme-producing for the different pH.
Specific embodiment
Further illustrate the present invention with reference to instantiation.
Embodiment 1
Fresh sargassum thunbergii after clean drying is worn into 50～60 mesh powder；
Marine algae powder and water are according to mass ratio 4:100 load in fermentation tank, and sterilize under the conditions of 115 DEG C 25min, obtains product A；
And prepare seed liquor：Bacillus megaterium YIC～the BM1 of cellulase-producing is seeded to fluid nutrient medium, cultivates 24 at 30 DEG C
Hour；The preserving number of the bacillus megaterium YIC～BM1 of described cellulase-producing is CGMCC No.12156；Cellulase-producing
Bacillus megaterium YIC～BM1 and culture medium volume ratio be 5:100.Seed liquor is added in product A, wherein plants
Sub- liquid is 10 with the mass ratio of product A:100, obtain product B；It is 30 DEG C in temperature, to product B under conditions of pH6.5～7.0
Fermented, fermentation time is 8 days, obtains products C；The pH to 5.0～6.0 of adjustment products C, and with per hour 3 DEG C of temperature
Change adjustment temperature, to 5 DEG C, preserves 12h, obtains product D；With the temperature of per hour 3 DEG C of temperature change adjustment product D to 30
DEG C, pH to 6.5～7.0, then supplements seed liquor, the seed liquor supplemented is 5 with the mass ratio of product D:100, preserve 12h, obtain
To product E；The pH to 5.0～6.0 of adjustment product E, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 12h,
Obtain product F；The temperature of product F is adjusted to 30 DEG C with per hour 3 DEG C of temperature change, pH to 6.5～7.0, then supplements and plants
Sub- liquid, the seed liquor supplemented is 5 with the mass ratio of product F:100, preserve 12h, obtain product G；Obtain huge after product G is air-dried
Bacterium anthracoides microbial inoculum；Amino acid organic matter and bacillus megaterium microbial inoculum will be contained according to mass ratio 96:After 4 mix, contained
There is the Water soluble fertilizer of bacillus megaterium and amino acid.
Embodiment 2
By the fresh Sargassum horneri after clean drying and new fresh sargassum according to mass ratio 1:1 compounds, and wears into 50～60 mesh powder；
Marine algae powder and water are according to mass ratio 6:100 load in fermentation tank, and sterilize under the conditions of 115 DEG C 25min, obtains product A；
And prepare seed liquor：Bacillus megaterium YIC～the BM1 of cellulase-producing is seeded to fluid nutrient medium, cultivates 24 at 35 DEG C
Hour；The preserving number of the bacillus megaterium YIC～BM1 of described cellulase-producing is CGMCC No.12156；Cellulase-producing
Bacillus megaterium YIC～BM1 and culture medium volume ratio be 5:100.Seed liquor is added in product A, wherein plants
Sub- liquid is 10 with the mass ratio of product A:100, obtain product B；It is 35 DEG C in temperature, to product B under conditions of pH6.0～6.5
Fermented, fermentation time is 8 days, obtains products C；The pH to 5.5～6.0 of adjustment products C, and with per hour 3 DEG C of temperature
Change adjustment temperature, to 5 DEG C, preserves 12h, obtains product D；With the temperature of per hour 3 DEG C of temperature change adjustment product D to 35
DEG C, pH to 6.0～6.5, then supplements seed liquor, the seed liquor supplemented is 6 with the mass ratio of product D:100, preserve 12h, obtain
To product E；The pH to 5.5～6.0 of adjustment product E, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 12h,
Obtain product F；The temperature of product F is adjusted to 35 DEG C with per hour 3 DEG C of temperature change, pH to 6.0～6.5, then supplements and plants
Sub- liquid, the seed liquor supplemented is 6 with the mass ratio of product F:100, preserve 12h, obtain product G；Obtain huge after product G is air-dried
Bacterium anthracoides YIC～BM1 microbial inoculum；Amino acid organic matter and bacillus megaterium microbial inoculum will be contained according to mass ratio 96:4 mixings
Afterwards, obtain the Water soluble fertilizer containing bacillus megaterium and amino acid.
Embodiment 3
New fresh sargassum after clean drying is worn into 50～60 mesh powder；
Marine algae powder and water are according to mass ratio 8:100 load in fermentation tank, and sterilize under the conditions of 115 DEG C 25min, obtains product A；
And prepare seed liquor：Bacillus megaterium YIC～the BM1 of cellulase-producing is seeded to fluid nutrient medium, cultivates 24 at 30 DEG C
Hour；The preserving number of the bacillus megaterium YIC～BM1 of described cellulase-producing is CGMCC No.12156；Cellulase-producing
Bacillus megaterium YIC～BM1 and culture medium volume ratio be 5:100.Seed liquor is added in product A, wherein plants
Sub- liquid is 10 with the mass ratio of product A:100, obtain product B；It is 30 DEG C in temperature, to product B under conditions of pH6.5～7.0
Fermented, fermentation time is 8 days, obtains products C；The pH to 5.0～6.0 of adjustment products C, and with per hour 3 DEG C of temperature
Change adjustment temperature, to 5 DEG C, preserves 12h, obtains product D；With the temperature of per hour 3 DEG C of temperature change adjustment product D to 30
DEG C, pH to 6.5～7.0, then supplements seed liquor, the seed liquor supplemented is 5 with the mass ratio of product D:100, preserve 12h, obtain
To product E；The pH to 5.0～6.0 of adjustment product E, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 12h,
Obtain product F；The temperature of product F is adjusted to 30 DEG C with per hour 3 DEG C of temperature change, pH to 6.5～7.0, then supplements and plants
Sub- liquid, the seed liquor supplemented is 8 with the mass ratio of product F:100, preserve 12h, obtain product G；Obtain huge after product G is air-dried
Bacterium anthracoides YIC～BM1 microbial inoculum；Amino acid organic matter and bacillus megaterium microbial inoculum will be contained according to mass ratio 96:4 mixings
Afterwards, obtain the Water soluble fertilizer containing bacillus megaterium and amino acid.
Embodiment 4
New fresh sargassum after clean drying is worn into 50～60 mesh powder；
Marine algae powder and water are according to mass ratio 10:100 load in fermentation tank, and sterilize under the conditions of 115 DEG C 25min, obtains product
A；And prepare seed liquor：Bacillus megaterium YIC～the BM1 of cellulase-producing is seeded to fluid nutrient medium, cultivates at 30 DEG C
24 hours；The preserving number of the bacillus megaterium YIC～BM1 of described cellulase-producing is CGMCC No.12156；Produce cellulose
The volume ratio of the bacillus megaterium YIC～BM1 of enzyme and culture medium is 5:100.Seed liquor is added in product A, wherein
Seed liquor is 10 with the mass ratio of product A:100, obtain product B；It is 30 DEG C in temperature, to product under conditions of pH6.5～7.0
B is fermented, and fermentation time is 8 days, obtains products C；The pH to 5.0～6.0 of adjustment products C, and with per hour 3 DEG C of temperature
Change adjustment temperature, to 5 DEG C, preserves 12h, obtains product D；With the temperature of per hour 3 DEG C of temperature change adjustment product D to 30
DEG C, pH to 6.5～7.0, then supplements seed liquor, the seed liquor supplemented is 6 with the mass ratio of product D:100, preserve 12h, obtain
To product E；The pH to 5.0～6.0 of adjustment product E, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 12h,
Obtain product F；The temperature of product F is adjusted to 30 DEG C with per hour 3 DEG C of temperature change, pH to 6.5～7.0, then supplements and plants
Sub- liquid, the seed liquor supplemented is 8 with the mass ratio of product F:100, preserve 12h, obtain product G；Obtain huge after product G is air-dried
Bacterium anthracoides YIC～BM1 microbial inoculum；Amino acid organic matter and bacillus megaterium microbial inoculum will be contained according to mass ratio 96:4 mixings
Afterwards, obtain the Water soluble fertilizer containing bacillus megaterium and amino acid.
In embodiment 1 to 4, described organic matter containing amino acid is sodium glutamate and potassium fulvate mass ratio 7:89 combination
Thing.
Comparative example 1
Do not adjust the temperature in sweat and pH value, keep the original temperature of environment and the pH value of material itself, other bars
Part and material proportion are consistent with embodiment 2, and operating procedure is with embodiment 2.
Comparative example 2
Obstructed everfermentation, without microbial inoculum, is compounded with the potassium fulvate of 4 parts of marine algae extracts, 17 parts of sodium glutamate and 79 parts
Obtain composition.Described marine algae extract is obstructed everfermentation technique, and that is extracted from marine alga by way of soaking and dissolving can
Dissolubility organic principle.
Product checking：According to People's Republic of China (PRC) agricultural industry criteria NY/T 798-2015, People's Republic of China's agriculture
Industry professional standard NY/T 1429-2010 detects, testing result is shown in Table 1 to product indices.
Table 1：The embodiment of the present invention and the quality measurements of comparative example
Note：Record the bacillus megaterium that the effective viable bacteria in soil refers to present invention employing.
Result above shows, the microbial manure indices that the present invention produces are obviously higher than national standard, Er Qiejing
Everfermentation is processed, and in fertilizer, the content of heavy metal substantially reduces, and miscellaneous bacteria rate substantially reduces, and the zymotechnique that the present invention adopts is permissible
Living bacteria count in product is greatly improved, with the obvious advantage in like product.
Embodiments of the invention and comparative example related fertilizer are imposed on tomato planting plot, mu consumption is 40kg.
Planting season is 1 year, and soil property specific performance index is shown in Table 2.
Table 2：Property indices after soil improvement
Note：Record the bacillus megaterium that the effective viable bacteria in soil refers to present invention employing.
Result shows：The present invention adopt bacterial classification survive in soil and grows, soil function be improved significantly, ventilate
Porosity is 12.9%-14.2%, soil pH 6.5-6.6, soil weight 1.31-1.41g/cm3, content of beary metal effectively reduces.
Apply the embodiment and comparative example product impact situation to tomato crop in the present invention, with the bacterium applying market purchase
Fertile class product contrast, fertilizer efficiency the results are shown in Table 4.
Table 4：Fertilizer efficiency comparing result
| Project | Output increased | Malformed fruit rate reduces | Commodity fruit rate improves | Freeze injury rate reduces | Insect pest rate reduces | Disease rate reduces |
| Embodiment 1 | 7.5% | 6.5% | 10.9% | 11% | 16% | 15% |
| Embodiment 2 | 8.7% | 6.9% | 11.5% | 14% | 20% | 17% |
| Embodiment 3 | 8.5% | 6.7% | 11.2% | 12% | 18% | 16% |
| Embodiment 4 | 8.2% | 6.7% | 11.0% | 11% | 17% | 14% |
| Comparative example 1 | 5.9% | 2.2% | 5.2% | 5% | 7% | 9% |
| Comparative example 2 | 5.7% | 2.0% | 4.9% | 4% | 6% | 7% |
Result shows：In the case that dose is suitable, the microbiological compound fertilizer product using the present invention makees article hence it is evident that being lifted
Matter, greatly reduces effect pest and disease damage, effectively strengthens the resistance of crop, can raising effect yield 7.5%-8.7%.
The preserving number of described bacillus megaterium YIC～BM1 for CGMCC No.12156 depositary institution title is：China is micro-
Biological inoculum preservation administration committee common micro-organisms center；Depositary institution address is：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
No. 3；Preservation date：On March 2nd, 2016；Latin Classification And Nomenclature is：Bacillus megaterium.
The biological nature of described bacillus megaterium YIC～BM1 is as follows：
Cellulose culture medium：Sodium cellulose glycolate 15g, ammonium nitrate 1g, magnesium sulfate 1g, potassium dihydrogen phosphate 2g, agar 20g, steam
Distilled water 1000ml.
Liquid fermentation medium：Sodium cellulose glycolate 10g, peptone 3g, dusty yeast 0.5g, ammonium nitrate 2g, magnesium sulfate
0.3g, potassium dihydrogen phosphate 2g, calcium chloride 0.3g, distilled water 1000ml, pH7.0-7.4.
After YIC～BM1 bacterial strain grows 48h on cellulose culture medium flat plate, bacterium colony is circular, white, surfacing.Micro-
Sem observation bacterial strain is Gram-positive, shaft-like, can move, and produces gemma.20～40 DEG C of growth temperature range, PH scope 6-10,
NaCl concentration scope 0～20%, 37 DEG C of bacterial strain optimum growth temperature, the most suitable growth pH 7.5.Catalase, oxidizing ferment, galactosidase
The positive, indoles, VP experiment is negative, does not go back orthonitric acid；Glucose, mannose, lactose, gossypose, sucrose, Arab can be utilized
Sugar, wood sugar, mannitol, sorbierite.According to its physiological and biochemical property, this identification of strains is bacillus megateriumBacillus megaterium.
Fig. 1 finds out, YIC～BM1 bacterial strain is gram-positive bacteria；
Fig. 2 finds out, the affiliation of YIC～BM1 bacterial strain is nearest with bacillus megaterium；
Fig. 3 finds out, the most suitable producing enzyme temperature of YIC～BM1 bacterial strain is 35 DEG C；
Fig. 4 finds out, optimal producing enzyme pH of YIC～BM1 bacterial strain is 6.0.
Claims (10)
1. a kind of production method containing bacillus megaterium microbial inoculum and the Water soluble fertilizer of amino acid is it is characterised in that according to following step
Suddenly carry out：
Amino acid organic matter and bacillus megaterium microbial inoculum will be contained according to mass ratio 40～99:After 1～60 mixes, obtain containing huge
Bacterium anthracoides and the Water soluble fertilizer of amino acid；
One of resultant product after wherein organic matter containing amino acid is extraction monosodium glutamate, sodium glutamate and potassium fulvate or any
Ratio two or more；
Wherein bacillus megaterium microbial inoculum is prepared from according to following steps：
1）, prepare marine algae powder：Marine alga after drying is clayed into power；
2）, prepare product A：Marine algae powder and water are according to mass ratio 1～10:100 load in fermentation tank, in 100～150 DEG C of conditions
Lower sterilizing 15～35min, obtains product A；
And prepare seed liquor：Bacillus megaterium is seeded to fluid nutrient medium, cultivates 20～30 hours at 25 DEG C～40 DEG C；Huge
Volume ratio between Bacterium anthracoides and culture medium is 1～10:100；
3）, prepare product B：Seed liquor is added in product A and obtains product B, wherein seed liquor and the mass ratio of product A are 1
～10:100；
4）, preparation products C：It is 25 DEG C～40 DEG C in temperature, under conditions of pH5～9, product B is fermented, fermentation time is 6
～10 days, obtain products C；
5）, prepare product D：The pH to 5.0～6.0 of adjustment products C, temperature, to 3～10 DEG C, preserves 3h～72h, obtains product D；
6）, preparation product E:Adjustment product D temperature to 25 DEG C～40 DEG C, pH to 5～9, then supplement seed liquor, supplement kind
Sub- liquid is 1～10 with the mass ratio of product D:100, preserve 3h～72h, obtain product E；
7）, preparation product F：The pH to 5.0～6.0 of adjustment product E, temperature, to 3～10 DEG C, preserves 3h～72h, obtains product F；
8）, preparation product G：Adjustment product F temperature to 25 DEG C～40 DEG C, pH to 5～9, then supplement seed liquor, supplement kind
Sub- liquid is 1～10 with the mass ratio of product F:100, preserve 3h～72h, obtain product G；
9）, prepare the bacillus megaterium YIC～BM1 microbial inoculum of cellulase-producing：Cellulase-producing is obtained after product G is air-dried
Bacillus megaterium microbial inoculum.
2. the production method containing bacillus megaterium microbial inoculum and the Water soluble fertilizer of amino acid as claimed in claim 1, its feature
It is：Described fluid nutrient medium is made into according to following mass ratio：5 parts of sodium alginate, 5 parts of ammonium sulfate, 1 part of magnesium sulfate, phosphoric acid hydrogen
2 parts of dipotassium, 0.01 part of ferrous sulfate, 20 parts of agar and 1000 parts of distilled water.
3. the production method containing bacillus megaterium microbial inoculum and the Water soluble fertilizer of amino acid as claimed in claim 1, its feature
It is step 2）The mass ratio of described marine algae powder and water is 4:100；115 DEG C of sterilising temp, sterilization time 25min；Preparation kind
During sub- liquid, bacillus megaterium is seeded in fluid nutrient medium, cultivates 22 hours, obtains seed liquor, bacillus megaterium and training
The volume ratio of foster base is 5:100；Step 5）With step 7）In, temperature is 5 DEG C, and the holding time is 12h.
4. the production method containing bacillus megaterium microbial inoculum and the Water soluble fertilizer of amino acid as claimed in claim 1, its feature
It is：Step 4）, step 6）With step 8）In, temperature is 25 DEG C, and pH is 6.5～7.0；Step 4）Fermentation time is 8 days；Step
6）With step 8）Holding time is 12h；Step 6）The mass ratio of middle fermentation seed liquid and product D and step 8）Middle fermentation seed
Liquid is 5 with the mass ratio of product F:100.
5. the production method containing bacillus megaterium microbial inoculum and the Water soluble fertilizer of amino acid as claimed in claim 1, its feature
It is：Step 5）, step 6）Step 7）With step 8）In, adjustment temperature is and adjusts 3 DEG C per hour.
6. the production method containing bacillus megaterium microbial inoculum and the Water soluble fertilizer of amino acid as claimed in claim 1, its feature
It is：Described organic matter containing amino acid is single amino acid, the salt of single amino acid, compound amino acid, compound amino acid
Salt, lysine factory and one of Gourmet Powder Factory's leftover bits and pieces, molasses fermented liquid and yeast factory leftover bits and pieces etc. or arbitrary proportion
Two kinds and more than.
7. the production method containing bacillus megaterium microbial inoculum and the Water soluble fertilizer of amino acid as claimed in claim 1, its feature
It is：Mass ratio between organic matter containing amino acid and bacillus megaterium microbial inoculum is 96:4.
8. the production containing bacillus megaterium microbial inoculum and the Water soluble fertilizer of amino acid as described in any one of claim 1-7
Method it is characterised in that：Described marine alga refers to the two or more of sargassum thunbergii, one of Sargassum horneri and sargassum or arbitrary proportion.
9. the production containing bacillus megaterium microbial inoculum and the Water soluble fertilizer of amino acid as described in any one of claim 1-7
Method it is characterised in that：Described bacillus megaterium is bacillus megaterium YIC～BM1, and preserving number is GMCC
No.12156.
10. the production method containing bacillus megaterium microbial inoculum and the Water soluble fertilizer of amino acid as claimed in claim 8, its feature
It is：Described bacillus megaterium is bacillus megaterium YIC～BM1, and preserving number is GMCC No.12156.
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| CN201610770117.7A CN106396907A (en) | 2016-08-31 | 2016-08-31 | Production method of water-soluble fertilizer containing Bacillusmegaterium fungicide and amino acids |
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| CN201610770117.7A CN106396907A (en) | 2016-08-31 | 2016-08-31 | Production method of water-soluble fertilizer containing Bacillusmegaterium fungicide and amino acids |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116041115A (en) * | 2022-10-14 | 2023-05-02 | 烟台绿云生物科技有限公司 | Water-soluble seaweed fertilizer containing bacillus californicus and polyglutamic acid as well as production method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262008A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation |
| CN104447008A (en) * | 2014-12-05 | 2015-03-25 | 中国科学院烟台海岸带研究所 | Method for preparing liquid seaweed fertilizer |
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2016
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104262008A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation |
| CN104447008A (en) * | 2014-12-05 | 2015-03-25 | 中国科学院烟台海岸带研究所 | Method for preparing liquid seaweed fertilizer |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116041115A (en) * | 2022-10-14 | 2023-05-02 | 烟台绿云生物科技有限公司 | Water-soluble seaweed fertilizer containing bacillus californicus and polyglutamic acid as well as production method and application thereof |
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