CN106348885A - Method for preparing organic liquid water-soluble fertilizer through combination of alkaline extraction and fermentation - Google Patents
Method for preparing organic liquid water-soluble fertilizer through combination of alkaline extraction and fermentation Download PDFInfo
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- CN106348885A CN106348885A CN201610776203.9A CN201610776203A CN106348885A CN 106348885 A CN106348885 A CN 106348885A CN 201610776203 A CN201610776203 A CN 201610776203A CN 106348885 A CN106348885 A CN 106348885A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
Abstract
The invention relates to a method for preparing an organic liquid water-soluble fertilizer through combination of alkaline extraction and fermentation. The method comprises the following steps: grinding dried algae into powder, putting into a fermenting tank, adding sodium carbonate, performing suction extraction, reacting for a period of time, adjusting the pH value of an extract, and sterilizing; inoculating a beef extract-peptone liquid culture medium with fermenting strains, namely bacillus megatherium YIC-BM1 and bacillus subtilis respectively, and culturing overnight to obtain seed liquid 1 and seed liquid 2; adding the seed liquid 1 and the seed liquid 2 into the fermenting tank according to a certain mass ratio, fermenting, adjusting a fermenting condition, keeping for a certain time, adjusting the fermenting condition again and supplementing the seed liquid, adjusting for three times to obtain a compound inoculum filtrate, adding water, compound inoculum liquid, a nitrogen fertilizer, a phosphate fertilizer, a potassium fertilizer and an organic substance-containing organic matter into a stirring tank, and stirring for dissolving completely to obtain the compound inoculum liquid containing organic liquid water-soluble fertilizer. By the method, the fermenting process is stable and controllable, the fermenting cycle is short, and the product efficacy is remarkable; the method is suitable for large-scale production.
Description
Technical field
The present invention relates to a kind of production method of liquid Water soluble fertilizer.
Background technology
Marine alga fertilizer, with natural seaweed as raw material, is processed through special biochemical process, extracts and retain marine alga natural activity
Composition, a kind of novel organic fertilizer material that refine concentrates and formed.Marine alga fertilizer has raising crop yield and quality, resists disease pest
Effect that evil and poor environment and improved soil conserve water and soil, it is applied to agricultural production the huge market demand and good
Development prospect.But, the quality of marine alga fertilizer to be ensured, it is necessary for getting hold of Sargassum raw material and the crucial ring of extraction process two
Section.
First, the quality of marine alga fertilizer quality depends on raw material used.At present, the raw material used by alga fertilizer manufacturing enterprise
Mainly based on Brown algae, including yellow tang, Thallus Laminariae (Thallus Eckloniae), Thallus Laminariae (Thallus Eckloniae), Alga Sgrgassi Enervess etc..Other algae such as Fucus Vesiculosus, Entermorpha, ulva, Kappa
Zao Dengyeyou minority enterprise with.External alga fertilizer enterprise is most to be raw material using Brown algae, is wherein especially applied with yellow tang
The most extensive such as famous brand name such as seasol, maxicrop, practical function effect also indicates that yellow tang is the Sargassum raw material of high-quality.
But, China coast does not have yellow tang to be distributed, and the raw material type that domestic enterprise adopts is numerous and diverse, and quality is irregular to differ, and causes Sargassum
Fertilizer product quality is very different, or even some producers are turned on the camouflage of " alga fertilizer ", directly blends alginic acid, fulvic acid, nitrogen phosphorus
Potassium etc. pretends to be alga fertilizer, have impact on alga fertilizer quality, has upset alga fertilizer market.
Secondly, the second factor of decision alga fertilizer quality is extraction process.Different extraction processes is to alga fertilizer activity
The species and content impact of composition is huge.At present, the extracting method of Sargassum active component mainly has chemical method, Physical and life
Thing method.Chemical method mainly uses acid, alkali and organic solvent and processes alginic cell, so that cell is cleared up or endogenous substance solubilising, should
Method is simple to operate, easily realizes, and is also the method that most alga fertilizer manufacturing enterprises adopt both at home and abroad.But, strong acid is strong
Acutely, the destruction to the active material ingredients in alginic cell is sizable to alkali reaction condition, and the organic reagent remaining
It is also potentially hazardous to environment.The principle of Physical is control pressure, the physical environmental condition such as temperature, makes sea using mechanical force
Frustule crushes, and content discharges.Although the method technique cleaning, environmental friendliness, the requirement for instrument and equipment is harsh,
Change in reaction conditions acutely, relatively costly it is difficult to realize large-scale production.Bioanalysises include enzymic degradation and fermentable two
Kind of method, its cardinal principle be destroy alginic cell wall construction and macromolecular substances of degrading easily absorb for plant little point
Sub- material, the method reaction is gentle, product diversification, and whole production process safety and environmental protection is pollution-free, is preferable alga fertilizer life
Product method.
Research using Production by Microorganism Fermentation marine alga fertilizer is relatively fewer, and only several researchs are from multiple micro-
Biology carries out similar compost fermentation to Sargassum raw material or aerobic fermentation is processed, and there is sweat poor controllability, fermentation period is long,
The problems such as target product is indefinite is it is difficult to meet the unification of industrialization production requirements, stable, controlled standard, the quality of product
It is difficult to ensure.
Evaluation index for agricultural micro organism mainly includes the microbial inoculum quantity in the purity and unit mass of microbial inoculum.Cause
This, the bacterial preparation process as prior art is usually: carries out once under the conditions of the temperature of the suitable existence of microorganism and ph
Fermentation technology, is equivalent to hot-house culture.
Because the acidifying of current soil compaction is serious, outside air temperature is changeable, and the microbial bacterial agent of hot-house culture is directly thrown
It is put in soil, the soil environment because not adapting to severe causes mortality, even if having surviving because population cannot be formed on a small quantity
Effect, also cannot play the effect of microbial bacterial agent.
Content of the invention
The technical problem to be solved is to provide the side that a kind of alkali carries fermentation in combining prepares organic liquid Water soluble fertilizer
Method, first, Sargassum of being degraded using the bacillus megaterium of function admirable, and simulate soil natural ecological environment, after making fermentation
Bacillus megaterium, after putting into soil, can adapt to environment quickly it is ensured that survival rate；Secondth, provide and utilize described compound bacteria system
The method of standby liquid Water soluble fertilizer, the method sweat is stablized controlled, and fermentation period is short, and product efficiency is notable, is suitable to scale
Produce；3rd, add bacillus subtilises in seed liquor, serve synergism, be more beneficial for bacillus megaterium
Growth
In order to solve above-mentioned technical problem, the present invention employs the following technical solutions:
A kind of alkali carries fermentation in combining prepares the method for organic liquid Water soluble fertilizer it is characterised in that following the steps below:
By water, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, Organic substance and compound bacteria filtrate according to mass ratio 10～40:10～50:10～50:
10～50:10～20:1～60 are added in stirred tank, after stirring and dissolving, obtain organic liquid Water soluble fertilizer；
Wherein Organic substance is the two or more of one of shitosan, molasses, citric acid or arbitrary proportion；
Wherein compound bacteria filtrate is prepared from according to following steps:
1), prepare marine algae powder: the Sargassum after drying is clayed into power；
2), prepare product a: marine algae powder, sodium carbonate loads fermentation with water according to mass ratio 1～10:0.25-2.5:100
In tank, under the conditions of 60 DEG C, react 120～180min, adjust its ph in 7.2-7.4, then under the conditions of 100～150 DEG C
Sterilizing 15～35min, obtains product a；
And prepare seed liquor: bacillus megaterium and bacillus subtilises are seeded in fluid medium respectively, 25
～40 DEG C of cultures obtain seed liquor 1 and seed liquor 2 for 20～30 hours；Seed liquor 1 and seed liquor 2 are according to volume ratio 1～5:1～5
It is mixed to get seed mixture liquid；
Two kinds of volume ratios between bacterium and culture medium are 1～10:100；
3), prepare product b: seed mixture liquid is added in product a and obtains product b, wherein seed mixture liquid and product a
Mass ratio be 1～10:100；
4), prepare product c: be 25 DEG C～40 DEG C in temperature, under conditions of ph5～9, product b fermented, during fermentation
Between be 6～10 days, obtain product c；
5), prepare product d: the ph to 5.0～6.0 of adjustment product c, temperature, to 3～10 DEG C, preserves 3h～72h, produced
Product d；
6), prepare product e: the temperature of adjustment product d to 25 DEG C～40 DEG C, ph to 5～9, then supplement seed mixture liquid,
The seed mixture liquid supplementing is 1～10:100 with the mass ratio of product d, preserves 3h～72h, obtains product e；
7), prepare product f: the ph to 5.0～6.0 of adjustment product e, temperature, to 3～10 DEG C, preserves 3h～72h, produced
Product f；
8), prepare product g: the temperature of adjustment product f to 25 DEG C～40 DEG C, ph to 5～9, then supplement seed mixture liquid,
The seed mixture liquid supplementing is 1～10:100 with the mass ratio of product f, preserves 3h～72h, obtains product g；
9), prepare compound bacteria filtrate: obtain compound bacteria filtrate by after product g filter pressing.
Described fluid medium is made into according to following mass ratio: 5 parts of peptone, 5 parts of Carnis Bovis seu Bubali cream, 5 parts of ammonium sulfate, magnesium sulfate
1 part, 2 parts of dipotassium hydrogen phosphate, 0.01 part of ferrous sulfate and 1000 parts of distilled water.
Step 2) described marine algae powder, sodium carbonate and water mass ratio be 4:1:100；115 DEG C of sterilising temp, sterilization time
25min；During preparation seed liquor, bacillus megaterium and bacillus subtilises are seeded in fluid medium respectively, and culture 22 is little
When obtain seed liquor 1 and seed liquor 2, the volume ratio of two kinds of bacterium total amounts and culture medium is 5:100；Step 5) and step 7) in,
Temperature is 5 DEG C, and the holding time is 12h.
Step 4), step 6) and step 8) in, temperature be 25 DEG C, ph be 6.5～7.0；Step 4) fermentation time be 8 days；
Step 6) and step 8) holding time be 12h；Step 6) in the mass ratio of seed mixture liquid and product d that supplements and step 8)
The seed mixture liquid of middle supplement and the mass ratio of product f are 5:100.
Step 5), step 6) step 7) and step 8) in, adjustment temperature be per hour adjust 3 DEG C.
Described nitrogenous fertilizer is ammonium nitrate urea liquid, and phosphate fertilizer is APP, and potash fertilizer is dipotassium hydrogen phosphate.
Described Sargassum refer to two kinds of sargassum thunbergii, Thallus Laminariae (Thallus Eckloniae), opotism, one of Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) and Alga Sgrgassi Enervess or arbitrary proportion with
On.
Described bacillus megaterium is bacillus megaterium yic～bm1, and preserving number is cgmcc no.12156.
Invent and there are following Advantageous Effects:
1st, sweat of the present invention is stablized controlled, and fermentation period is short, and product efficiency is notable, is suitable to large-scale production.
2nd, the fermentation liquid that the present invention obtains has good water solubility it is easy to plant absorption utilizes, simultaneously as a kind of important
Signaling molecule, is capable of growth regulating and the induction resistance mechanism of involved in plant, promotes the growth of plant, improve plant to bad ring
Border and the resistance of pest and disease damage.
3rd, the present invention makes the bacterium that fermentation goes out possess the ability adapting to severe soil environment by adjusting fermentation condition, more has
Performance beneficial to bacterium function.
4th, the present invention is by slow temperature adjustment, makes the change of compound bacteria gradually adaptive temperature, it is to avoid temperature change mistake
Hurry up, compound bacteria does not adapt to and mortality.
6. increase single step reaction before present invention fermentation, carry out alkali carries with sodium carbonate, reaction condition is relatively mild, after alkali carries again
Fermented, the content of core active material brown alga oligose can be significantly improved, improved the release of the endogenous organic substance of Sargassum itself.
Brief description
Fig. 1 is the Gram’s staining of microexamination yic～bm1 bacterial strain and assembles agglomerating phenomenon figure.
Yic～bm1 bacterial strain phylogenetic tree schematic diagram based on 16srdna sequence construct for the Fig. 2.
Fig. 3 is the schematic diagram of the impact to yic～bm1 strain enzyme-producing for the different temperatures.
Fig. 4 is the impact schematic diagram to yic～bm1 strain enzyme-producing for the different ph.
Specific embodiment
Further illustrate the present invention with reference to instantiation.
Embodiment 1
Fresh sargassum thunbergii after clean drying is worn into 50～60 mesh powder；
Marine algae powder, sodium carbonate and water load in fermentation tank according to mass ratio 5:1:100, react under the conditions of 60 DEG C
120min, with glacial acetic acid adjustment ph 7.2～7.4, then the 20min that sterilizes under the conditions of 120 DEG C, obtain product a；
And prepare seed liquor: bacillus megaterium yic～bm1 and bacillus subtilises are seeded to fluid medium respectively
Culture 20 hours；The preserving number of described bacillus megaterium yic～bm1 is cgmcc no.12156；Described fluid medium is:
Peptone 5g, 5 parts of Carnis Bovis seu Bubali cream, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g and distilled water 1000ml
Mixture, ph7.2～7.4；The volume ratio of bacillus megaterium yic～bm1 and culture medium is 2:100, bacillus subtilis
The volume ratio of bacterium and culture medium is 1:100.Two kinds of seed liquor are added to as seed mixture liquid according to the volume ratio mixing of 1:1
In product a, wherein seed mixture liquid and the mass ratio of product a are 2:100, obtain product b；It is 20 DEG C in temperature, ph5.5～
Under conditions of 6.0, product b is fermented, fermentation time is 7 days, obtains product c；The ph to 5.0～6.0 of adjustment product c, and
With per hour 20 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 24h, obtain product d；With per hour 20 DEG C of temperature change
The temperature of adjustment product d to 20 DEG C, ph to 5.5～6.0, then supplement seed mixture liquid, seed mixture liquid and the product d supplementing
Mass ratio be 1:100, preserve 24h, obtain product e；The ph to 5.0～6.0 of adjustment product e, and with per hour 20 DEG C of temperature
Degree change adjustment temperature, to 5 DEG C, preserves 24h, obtains product f；
The temperature of product f is adjusted to 20 DEG C with per hour 20 DEG C of temperature change, ph to 5.5～6.0, then supplements mixing
Seed liquor, the seed mixture liquid supplementing is 1:100 with the mass ratio of product f, preserves 24h, obtains product g；After product g filter pressing
Obtain compound bacteria filtrate；By water, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, molasses and compound bacteria filtrate according to mass ratio 40:10:10:10:20:
After 10 are added to dissolving completely in stirred tank, obtain the organic liquid Water soluble fertilizer containing compound bacteria.
Embodiment 2
Fresh Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) after clean drying and new fresh sargassum are compounded according to mass ratio 1:1, wears into 50～60 mesh powder；
Marine algae powder, sodium carbonate and water load in fermentation tank according to mass ratio 2:0.5:100, react under the conditions of 60 DEG C
120min, with glacial acetic acid adjustment ph 7.2～7.4, then the 30min that sterilizes under the conditions of 115 DEG C, obtain product a；And prepare seed
Liquid: bacillus megaterium yic～bm1 and bacillus subtilises are seeded to fluid medium respectively, cultivate 24 hours at 35 DEG C；
The preserving number of described bacillus megaterium yic～bm1 is cgmcc no.12156；Bacillus megaterium yic～bm1 and culture medium
Volume ratio be 2:100, the volume ratio of bacillus subtilises and culture medium is 1:100.By two kinds of seed liquor according to 2:1 body
The mixing of long-pending ratio is added in product a as seed mixture liquid, and wherein mixing seed liquor and the mass ratio of product a are 5:100, obtain
Product b；It is 37 DEG C in temperature, under conditions of ph6.0～6.5, product b is fermented, fermentation time is 8 days, obtains product c；
The ph to 5.5～6.0 of adjustment product c, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 12h, obtain product
d；The temperature of product d is adjusted to 35 DEG C with per hour 3 DEG C of temperature change, ph to 6.0～6.5, then supplement mixing seed liquor,
The seed mixture liquid supplementing is 6:100 with the mass ratio of product d, preserves 12h, obtains product e；The ph to 5.5 of adjustment product e～
6.0, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 12h, obtain product f；With per hour 3 DEG C of temperature
Change adjustment product f temperature to 35 DEG C, ph to 6.0～6.5, then supplement seed mixture liquid, supplement seed mixture liquid with
The mass ratio of product f is 6:100, preserves 12h, obtains product g；Obtain compound bacteria filtrate by after product g filter pressing；By water, nitrogenous fertilizer,
Phosphate fertilizer, potash fertilizer, molasses and compound bacteria filtrate are added in stirred tank according to mass ratio 40:10:10:10:20:10 dissolves completely
Afterwards, obtain the organic liquid Water soluble fertilizer containing compound bacteria.
Embodiment 3
New fresh sargassum after clean drying is worn into 50～60 mesh powder；
Marine algae powder, sodium carbonate and water load in fermentation tank according to mass ratio 6:1.5:100, react under the conditions of 60 DEG C
120min, with glacial acetic acid adjustment ph 7.2～7.4, then the 20min that sterilizes under the conditions of 125 DEG C, obtain product a；And prepare seed
Liquid: bacillus megaterium yic～bm1 and bacillus subtilises are seeded to fluid medium respectively and cultivate 24 hours；Described huge
The preserving number of Bacterium anthracoides yic～bm1 is cgmcc no.12156；Described fluid medium is: peptone 5g, Carnis Bovis seu Bubali cream 5
Part, ammonium sulfate 5g, magnesium sulfate 1g, the mixture of dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g and distilled water 1000ml, ph7.2～
7.4；The volume ratio of bacillus megaterium yic～bm1 and culture medium is 1:100, the volume of bacillus subtilises and culture medium
Than for 1:100.Using two kinds of seed liquor according to 1:2 mixing be added in product a as seed mixture liquid, wherein seed mixture liquid with
The mass ratio of product a is 2:100, obtains product b；It is 30 DEG C in temperature, under conditions of ph7～7.5, product b is fermented,
Fermentation time is 9 days, obtains product c；The ph to 5.0～6.0 of adjustment product c, and temperature is adjusted with per hour 1 DEG C of temperature change
Spend to 5 DEG C, preserve 18h, obtain product d；The temperature of product d is adjusted to 30 DEG C with per hour 8 DEG C of temperature change, ph to 7～
7.5, then supplement seed mixture liquid, the seed mixture liquid supplementing is 4:100 with the mass ratio of product d, preserves 18h, is produced
Product e；The ph to 5.0～6.0 of adjustment product e, and with per hour 1 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 18h, obtain
Product f；
The temperature of product f is adjusted to 30 DEG C with per hour 8 DEG C of temperature change, ph to 7.0～7.5, then supplements mixing
Seed liquor, the seed mixture liquid supplementing is 4:100 with the mass ratio of product f, preserves 18h, obtains product g；After product g filter pressing
Obtain compound bacteria filtrate；By water, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, molasses and compound bacteria filtrate according to mass ratio 40:10:10:10:20:
After 10 are added to dissolving completely in stirred tank, obtain the organic liquid Water soluble fertilizer containing compound bacteria.
Embodiment 4
New fresh sargassum after clean drying is worn into 50～60 mesh powder；
Marine algae powder, sodium carbonate and water load in fermentation tank according to mass ratio 8:2:100, react under the conditions of 60 DEG C
120min, with glacial acetic acid adjustment ph 7.2～7.4, then the 15min that sterilizes under the conditions of 140 DEG C, obtain product a；And prepare seed
Liquid: bacillus megaterium yic～bm1 and bacillus subtilises are seeded to fluid medium respectively and cultivate 20 hours；Described huge
The preserving number of Bacterium anthracoides yic～bm1 is cgmcc no.12156；Described fluid medium is: peptone 5g, Carnis Bovis seu Bubali cream 5
Part, ammonium sulfate 5g, magnesium sulfate 1g, the mixture of dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g and distilled water 1000ml, ph7.2～
7.4；The volume ratio of bacillus megaterium yic～bm1 and culture medium is 4:100, the volume of bacillus subtilises and culture medium
Than for 1:100.Two kinds of seed liquor are added in product a as seed mixture liquid according to the mixing of 1:5 volume ratio, wherein hybrid
Sub- liquid is 8:100 with the mass ratio of product a, obtains product b；It is 35 DEG C in temperature, under conditions of ph6.5～7.0, product b is entered
Row fermentation, fermentation time obtained product c for 9 days；The ph to 5.0～6.0 of adjustment product c, and with per hour 3 DEG C of temperature change
Adjustment temperature, to 5 DEG C, preserves 12h, obtains product d；The temperature of product d is adjusted to 35 DEG C with per hour 3 DEG C of temperature change, ph
To 6.5～7.0, then supplement seed mixture liquid, the seed mixture liquid supplementing is 8:100 with the mass ratio of product d, preserves 12h,
Obtain product e；The ph to 5.0～6.0 of adjustment product e, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve
12h, obtains product f；
The temperature of product f is adjusted to 35 DEG C with per hour 3 DEG C of temperature change, ph to 6.5～7.0, then supplements mixing
Seed liquor, the seed mixture liquid supplementing is 8:100 with the mass ratio of product f, preserves 12h, obtains product g；After product g filter pressing
Obtain compound bacteria filtrate；By water, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, molasses and compound bacteria filtrate according to mass ratio 40:10:10:10:20:
After 10 are added to dissolving completely in stirred tank, obtain the organic liquid Water soluble fertilizer containing compound bacteria.
Comparative example 1
Do not adjust the temperature in sweat and ph value, keep the original temperature of environment and the ph value of material itself, other
Condition and material proportion consistent with embodiment 2, operating procedure is with embodiment 2.
Comparative example 2
Do not pass through alkali carries and fermentation, without microbial inoculum, microbial bacterial agent is substituted with Sargassum extract, by water, nitrogenous fertilizer, phosphorus
Fertilizer, potash fertilizer, Organic substance and solubility Sargassum extract are added to dissolving in stirred tank according to mass ratio 40:10:10:10:20:10
After completely, obtain Sargassum organic liquid Water soluble fertilizer.
Embodiment 1 to 4, in comparative example 1 to 2, described nitrogenous fertilizer is ammonium nitrate urea liquid, and phosphate fertilizer is APP, and potash fertilizer is
Dipotassium hydrogen phosphate；Described Sargassum extract is not pass through alkali carries and fermentation technology, by way of soaking and dissolving from Sargassum
The soluble organic fraction extracting.
Product checking: according to the company standard of organic water-soluble fertilizer, People's Republic of China (PRC) agricultural industry criteria ny/t
1106-2010 detects, testing result is shown in Table 1 to product indices.
Table 1: the quality measurements of the embodiment of the present invention and comparative example
Note: effectively viable bacteria refers to the compound bacteria of present invention employing.
Result above shows, the microbial manure indices that the present invention produces are obviously higher than national standard, Er Qiejing
Everfermentation is processed, and in fertilizer, the content of heavy metal substantially reduces, and miscellaneous bacteria rate substantially reduces, the alkali carries fermentation technology that the present invention adopts
Living bacteria count in product can be greatly improved, with the obvious advantage in like product.
Embodiments of the invention and comparative example related fertilizer are imposed on pepper planting plot, mu consumption is 40kg.
Planting season is 1 year, and soil property specific performance index is shown in Table 2.
Table 2: the property indices after soil improvement
Note: effectively viable bacteria refers to the compound bacteria of present invention employing.
Result shows: the present invention adopt strain survive in soil and grows, soil function be improved significantly, ventilate
Porosity is 12.9%-14.6%, soil ph 6.4-6.7, soil weight 1.38-1.42g/cm3, content of beary metal effectively drops
Low.
Apply the embodiment and comparative example product impact situation to Pepper crops in the present invention, with the bacterium applying market purchase
Fertile class product contrast, fertilizer efficiency the results are shown in Table 4.
Table 4: fertilizer efficiency comparing result
Result shows: in the case that dose is suitable, using the present invention organic liquid fertilizer product hence it is evident that lifting crop
Quality, greatly reduces effect pest and disease damage, effectively strengthens the resistance of crop, can raising effect yield 10.2%-10.9%.
The preserving number of described bacillus megaterium yic～bm1 is cgmcc no.12156；Depositary institution's title is: China
Microbiological Culture Collection administration committee common micro-organisms center；Depositary institution address is: BeiChen West Road, Chaoyang District, BeiJing City 1
Institute 3；Preservation date: on March 2nd, 2016；Latin Classification And Nomenclature is: bacillus megaterium.
The biological nature of described bacillus megaterium yic～bm1 is as follows:
Cellulose culture medium: Carboxymethyl cellulose sodium 15g, ammonium nitrate 1g, magnesium sulfate 1g, potassium dihydrogen phosphate 2g, agar
20g, distilled water 1000ml.
Liquid fermentation medium: Carboxymethyl cellulose sodium 10g, peptone 3g, yeast powder 0.5g, ammonium nitrate 2g, magnesium sulfate
0.3g, potassium dihydrogen phosphate 2g, calcium chloride 0.3g, distilled water 1000ml, ph7.0-7.4.
After yic～bm1 bacterial strain grows 48h on cellulose culture medium flat plate, bacterium colony is circular, white, surfacing.Micro-
Sem observation bacterial strain is Gram-positive, shaft-like, can move, and produces spore.20～40 DEG C of growth temperature range, ph scope 6-10,
Nacl concentration range 0～20%, 37 DEG C of bacterial strain optimum growth temperature, the most suitable growth ph 7.5.Catalase, oxidase, galactoside
Enzyme positive, indole, vp experiment is negative, does not go back orthonitric acid；Can utilize glucose, mannose, Lactose, Raffinose, sucrose, I
Uncle's sugar, xylose, Mannitol, Sorbitol.According to its physiological and biochemical property, this identification of strains is bacillus megaterium bacillus
megaterium.
Fig. 1 finds out, yic～bm1 bacterial strain is gram positive bacteria；
Fig. 2 finds out, the sibship of yic～bm1 bacterial strain is nearest with bacillus megaterium；
Fig. 3 finds out, the most suitable producing enzyme temperature of yic～bm1 bacterial strain is 35 DEG C；
Fig. 4 finds out, optimal producing enzyme ph of yic～bm1 bacterial strain is 6.0.
Claims (9)
1. a kind of alkali carries fermentation in combining prepares the method for organic liquid Water soluble fertilizer it is characterised in that following the steps below:
By water, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, Organic substance and compound bacteria filtrate according to mass ratio 10～40:10～50:10～50:10～
50:10～20:1～60 are added in stirred tank, after stirring and dissolving, obtain organic liquid Water soluble fertilizer；
Wherein Organic substance is the two or more of one of shitosan, molasses, citric acid or arbitrary proportion；
Wherein compound bacteria filtrate is prepared from according to following steps:
1), prepare marine algae powder: the Sargassum after drying is clayed into power；
2), prepare product a: marine algae powder, sodium carbonate loads in fermentation tank with water according to mass ratio 1～10:0.25-2.5:100,
React 120～180min under the conditions of 60 DEG C, adjust its ph in 7.2-7.4, then sterilize under the conditions of 100～150 DEG C
15～35min, obtains product a；
And prepare seed liquor: bacillus megaterium and bacillus subtilises are seeded in fluid medium respectively, 25～40
DEG C culture obtains seed liquor 1 and seed liquor 2 for 20～30 hours；Seed liquor 1 and seed liquor 2 mix according to volume ratio 1～5:1～5
Obtain seed mixture liquid；
Two kinds of volume ratios between bacterium and culture medium are 1～10:100；
3), prepare product b: seed mixture liquid is added in product a and obtains product b, wherein the matter of seed mixture liquid and product a
Amount ratio is 1～10:100；
4), prepare product c: be 25 DEG C～40 DEG C in temperature, under conditions of ph5～9, product b fermented, fermentation time is 6
～10 days, obtain product c；
5), prepare product d: the ph to 5.0～6.0 of adjustment product c, temperature, to 3～10 DEG C, preserves 3h～72h, obtains product d；
6), prepare product e: the temperature of adjustment product d to 25 DEG C～40 DEG C, ph to 5～9, then supplements seed mixture liquid, supplements
The mass ratio of seed mixture liquid and product d be 1～10:100, preserve 3h～72h, obtain product e；
7), prepare product f: the ph to 5.0～6.0 of adjustment product e, temperature, to 3～10 DEG C, preserves 3h～72h, obtains product f；
8), prepare product g: the temperature of adjustment product f to 25 DEG C～40 DEG C, ph to 5～9, then supplements seed mixture liquid, supplements
The mass ratio of seed mixture liquid and product f be 1～10:100, preserve 3h～72h, obtain product g；
9), prepare compound bacteria filtrate: obtain compound bacteria filtrate by after product g filter pressing.
2. alkali carries fermentation in combining as claimed in claim 1 prepare organic liquid Water soluble fertilizer method it is characterised in that: described liquid
Body culture medium is made into according to following mass ratio: 5 parts of peptone, 5 parts of Carnis Bovis seu Bubali cream, 5 parts of ammonium sulfate, 1 part of magnesium sulfate, dipotassium hydrogen phosphate
2 parts, 0.01 part of ferrous sulfate and 1000 parts of distilled water.
3. alkali carries fermentation in combining as claimed in claim 1 prepare organic liquid Water soluble fertilizer method it is characterised in that: step 2) institute
The mass ratio stating marine algae powder, sodium carbonate and water is 4:1:100；115 DEG C of sterilising temp, sterilization time 25min；Preparation seed liquor
When, bacillus megaterium and bacillus subtilises are seeded in fluid medium respectively, and culture obtains seed liquor 1 for 22 hours and plants
Sub- liquid 2, the volume ratio of two kinds of bacterium total amounts and culture medium is 5:100；In step 5) and step 7), temperature is 5 DEG C, the holding time
For 12h.
4. alkali carries fermentation in combining as claimed in claim 1 prepare organic liquid Water soluble fertilizer method it is characterised in that: step
4), in step 6) and step 8), temperature is 25 DEG C, and ph is 6.5～7.0；Step 4) fermentation time is 8 days；Step 6) and step
8) holding time is 12h；The mixing supplementing in the seed mixture liquid supplementing in step 6) and the mass ratio of product d and step 8)
Seed liquor is 5:100 with the mass ratio of product f.
5. alkali carries fermentation in combining as claimed in claim 1 prepare organic liquid Water soluble fertilizer method it is characterised in that: step
5), in step 6) step 7) and step 8), adjustment temperature is and adjusts 3 DEG C per hour.
6. alkali carries fermentation in combining as claimed in claim 1 prepare organic liquid Water soluble fertilizer method it is characterised in that: described nitrogen
Fertilizer is ammonium nitrate urea liquid, and phosphate fertilizer is APP, and potash fertilizer is dipotassium hydrogen phosphate.
7. the method that the alkali carries fermentation in combining as described in any one of claim 1-6 prepares organic liquid Water soluble fertilizer, it is special
Levy and be: described Sargassum refers to the two or more of sargassum thunbergii, Thallus Laminariae (Thallus Eckloniae), opotism, one of Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) and Alga Sgrgassi Enervess or arbitrary proportion.
8. the method that the alkali carries fermentation in combining as described in any one of claim 1-6 prepares organic liquid Water soluble fertilizer, it is special
Levy and be: described bacillus megaterium is bacillus megaterium yic～bm1, preserving number is cgmcc no.12156.
9. the method that the alkali carries fermentation in combining as described in any one of claim 7 prepares organic liquid Water soluble fertilizer, its feature
It is: described bacillus megaterium is bacillus megaterium yic～bm1, preserving number is cgmcc no.12156.
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| CN110256138A (en) * | 2019-04-17 | 2019-09-20 | 青岛明月蓝海生物科技有限公司 | A kind of clear liquid type water-soluble fertilizer and preparation method thereof |
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