CN106268556B - 一种蛋白纯化用磁珠的制备方法 - Google Patents
一种蛋白纯化用磁珠的制备方法 Download PDFInfo
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- CN106268556B CN106268556B CN201610674866.XA CN201610674866A CN106268556B CN 106268556 B CN106268556 B CN 106268556B CN 201610674866 A CN201610674866 A CN 201610674866A CN 106268556 B CN106268556 B CN 106268556B
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- Prior art keywords
- chitosan
- protein purification
- magnetic
- protein
- magnetic beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
- B01J13/06—Making microcapsules or microballoons by phase separation
- B01J13/14—Polymerisation; cross-linking
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610674866.XA CN106268556B (zh) | 2016-08-16 | 2016-08-16 | 一种蛋白纯化用磁珠的制备方法 |
Applications Claiming Priority (1)
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CN201610674866.XA CN106268556B (zh) | 2016-08-16 | 2016-08-16 | 一种蛋白纯化用磁珠的制备方法 |
Publications (2)
Publication Number | Publication Date |
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CN106268556A CN106268556A (zh) | 2017-01-04 |
CN106268556B true CN106268556B (zh) | 2021-06-08 |
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Family Applications (1)
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CN201610674866.XA Active CN106268556B (zh) | 2016-08-16 | 2016-08-16 | 一种蛋白纯化用磁珠的制备方法 |
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CN (1) | CN106268556B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109758989B (zh) * | 2019-03-08 | 2021-08-17 | 南京青柠生物科技有限公司 | 一种用于纯化组氨酸标记蛋白质的纳米磁珠的制备方法 |
CN115007118B (zh) * | 2022-08-08 | 2022-11-08 | 康盈红莓(中山)生物科技有限公司 | 蛋白质分离纯化用磁珠和其交联壳聚糖及其制备使用方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005087367A1 (ja) * | 2004-03-15 | 2005-09-22 | Hitachi Maxell, Ltd. | 磁性複合粒子およびその製造方法 |
CN101716494B (zh) * | 2009-11-18 | 2012-05-30 | 中国科学院生物物理研究所 | 一种纯化凝血酶的磁性亲和微球及其制备方法和应用 |
CN105170103B (zh) * | 2015-09-25 | 2017-10-31 | 哈尔滨工程大学 | 一种糠醛改性交联壳聚糖螯合树脂磁性颗粒及制备方法 |
CN105709698B (zh) * | 2016-04-07 | 2018-09-28 | 浙江科技学院 | 一种n-羧乙基壳聚糖纳米磁珠及其制备方法和应用 |
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2016
- 2016-08-16 CN CN201610674866.XA patent/CN106268556B/zh active Active
Non-Patent Citations (3)
Title |
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《N,N-二羧甲基壳聚糖的制备及其盐的抑菌作用研究》;王进涛等;《化学与生物工程》;20100531;第27卷(第5期);第35-38页 * |
《壳聚糖衍生物吸附剂在蛋白质分离纯化中的应用》;冯长根等;《离子交换与吸附》;20030630;第19卷(第3期);第284页第2-3行 * |
《磁性壳聚糖改性研究及其在水处理中的应用》;李海波;《中国优秀硕士学位论文全文数据库工程科技Ι辑》;20120115(第1期);第4页1.2.2节、第7-8页1.3.2节、第15页、第18页2.4.2节、第39-41页,图3.2 * |
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Publication number | Publication date |
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CN106268556A (zh) | 2017-01-04 |
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C06 | Publication | ||
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TA01 | Transfer of patent application right |
Effective date of registration: 20210519 Address after: 528400 1st floor, building D, no.6, Changlong North Street, Sanjiao Town, Zhongshan City, Guangdong Province Applicant after: Kangying red berry (Zhongshan) Biotechnology Co.,Ltd. Address before: 518100 8th floor, No.5 industrial lane, Taiping Road, Taiping Village, Ailian community, Longcheng street, Longgang District, Shenzhen City, Guangdong Province Applicant before: SHENZHEN REDBERRY BIOTECHNOLOGIES Ltd. |
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TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A preparation method of magnetic beads for protein purification Effective date of registration: 20220614 Granted publication date: 20210608 Pledgee: Bank of China Limited by Share Ltd. Zhongshan branch Pledgor: Kangying red berry (Zhongshan) Biotechnology Co.,Ltd. Registration number: Y2022980007751 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |