CN106265864A - A kind of compound probiotic for reproductive system and its production and use - Google Patents

A kind of compound probiotic for reproductive system and its production and use Download PDF

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CN106265864A
CN106265864A CN201610768383.6A CN201610768383A CN106265864A CN 106265864 A CN106265864 A CN 106265864A CN 201610768383 A CN201610768383 A CN 201610768383A CN 106265864 A CN106265864 A CN 106265864A
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reproductive system
compound probiotic
parts
powder
fructus
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王守亮
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    • A—HUMAN NECESSITIES
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    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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Abstract

The present invention relates to nursing materials technical field, it is specifically related to a kind of compound probiotic for reproductive system and its production and use, including the raw material components of following parts by weight: the Lactobacillus plantarum powder of 5～50 parts, the ferment powder of 1～10 part, the soybean fiber of 1～20 part and the sucrose of 0.5～5 part.Its each material composition compatibility of the compound probiotic for reproductive system of the present invention is reasonable, effectively microecological balance in regulation men and women's reproductive system, in addition, nutrient substance can also be produced, the opposing infection of bacterial virus and the function of some disease of prophylactic treatment, contribute to the self-regeneration in reproductive system, activation, maintenance, clear up, arrange and subtract deposit and strengthen reproductive system immunity etc..The preparation method of the present invention combines each material combination composition and determines, appropriate design fermentation step simultaneously, makes the finished product of preparation become apparent from for reproductive system nursing, health-care effect.

Description

A kind of compound probiotic for reproductive system and its production and use
Technical field
The present invention relates to nursing materials technical field, be specifically related to a kind of compound probiotic for reproductive system and system thereof Preparation Method and purposes.
Background technology
Probiotic bacteria is the active microorganism that a class is useful to host, is to be colonizated in human body intestinal canal, reproductive system, can produce Definite health efficacy thus improve host's microecological balance, play the active beneficial microorganism general name of beneficial effect.System genitale System, including male genital organ system and female sex organs system.Say accurately, refer on complex biological body any and sexual numerous Grow and form the tissue that reproductive system is relevant, due to various factors such as immunity degradation, genital system infection may be caused, especially It is women.Vagina is protected because of normal Tiny ecosystem (profitable strain, complete physiological structure, effective regulation of secretion function) Hold its powerful ability suppressing the invasion of external pathogenic bacteria, make vagina avoid courses of infection, but, because physiology, sexual activity, childbirth With the impact of the many factors such as hygienic habit, destroy the balance of Tiny ecosystem, cause vagina to be very easy to multi-infection.In dimension Holding in the factor of microecology in vaginas balance, estrogen, lactobacillus and vagina acid-base degree play vital effect.
Care product for men and women's reproductive system is varied in the market, adds including in care product The technical scheme of functional probiotic bacteria, such as application publication number are the patent of invention of CN 105663172 A, it is provided that a kind of ecological Gynecology externally used pharmaceutical combination and preparation method thereof, said composition by glucose, Tween 80, dipotassium hydrogen phosphate, sodium acetate, 7 points Sub-water of crystallization magnesium sulfate, manganese sulfate, Triammonium citrate, soluble starch, yeast extract are sterilized, inoculate probiotics bacterial liquid, through sending out After ferment, obtain lactic acid bacteria fermentation culture；In culture, add one or more pharmaceutically acceptable additives, i.e. obtain institute The ecological gynecology externally used pharmaceutical combination stated.Described ecological gynecology externally used pharmaceutical combination can be used for regulating the outer reproductive tract of women Ecological balance, prevent and treat female vagina.Through experiment, the pharmaceutical composition of the present invention is to staphylococcus aureus, large intestine Bacillus, Candida albicans are respectively provided with inhibitory or killing effect, to no skin irritation, have no skin allergic reaction.But the medicine of this formula Compositions, also needs in its material composition to add the additive such as preservative, excipient, and it directly acts on the system genitale of people for a long time System, can produce zest, be unfavorable for health.The pharmaceutical composition of this formula is imitated for the health care of human reproductive system simultaneously Fruit is still not enough, it is impossible to effectively strengthens the immunity of reproductive system, improves its repair ability.
As can be seen here, can be based on deficiency of the prior art, it is provided that a kind of containing profitable probliotics and for reproductive system Compound probiotic and preparation method thereof so that it is material composition takes from pure natural product, does not have any zest for human body Effect, and effectively provide corresponding preparation technology for this material composition, obtain the answering for reproductive system containing profitable probliotics Closing probiotic bacteria so that it is bacteria containing amount is suitable, using effect is optimal, effectively plays nursing and the work of health care for human reproductive system With, become the technical barrier that those skilled in the art are urgently to be resolved hurrily.
Summary of the invention
The present invention is to solve above-mentioned technical problem, it is provided that a kind of compound probiotic for reproductive system and preparation side thereof Method and purposes, it is good for the health-care effect of reproductive system, is effective against infection and some disease of prophylactic treatment of bacterial virus Sick function, strengthens reproductive system immunity, and its material composition has no side effect safely.
In order to reach above-mentioned technique effect, the present invention includes techniques below scheme:
A kind of compound probiotic for reproductive system, including the raw material components of following parts by weight: the plant of 5～50 parts Lactobacillus powder, the ferment powder of 1～10 part, the soybean fiber of 1～20 part and the sucrose of 0.5～5 part.
It is further preferred that the described compound probiotic for reproductive system includes the raw material components of following parts by weight: The Lactobacillus plantarum powder of 10～18 parts, the ferment powder of 2～3 parts, the soybean fiber of 2～5 parts and the sucrose of 2～3 parts.
The present invention each material composition compatibility is reasonable, effectively microecological balance in regulation men and women's reproductive system, simultaneously can also be Sweat produces nutrient substance, the infection of opposing bacterial virus and the function of some disease of prophylactic treatment, improves reproduction The immunologic function of system, inventive formulation belong to natural material composition, can life-time service, have no side effect and drug resistance produce.Its Middle Lactobacillus plantarum has a lot of health-care effects, as follows: 1, have certain immunoregulation effect；2, suppression is had to make to pathogenic bacterium With；3, serum cholesterol content and prevention cardiovascular disease are reduced；4, intestinal flora balance is maintained；5, promote that nutrient substance is inhaled Receive；6, lactose intolerance is alleviated；7, the formation etc. of tumor cell is suppressed.
Wherein soybean fiber mainly includes that cellulose, pectic substance, xylan, mannose etc. provide enough protein with effectively Increase viable bacteria amount；Ferment powder and sucrose are used to fermentation, and ferment powder has enhancing immunity, improve resistance, anti-inflammation and sterilization, it is provided that Protein catalysis viable bacteria amount.
Further, the described compound probiotic for reproductive system also includes the raw material components of following parts by weight: 2～ 30 parts of Fructus Hordei Germinatus powder, the Flos Caryophylli of 10～50 parts and the Flos Lonicerae of 0.5～10 part.
Wherein Fructus Hordei Germinatus powder is the supplement of dietary fiber, rich in rich in protein, vitamin, as Lactobacillus plantarum powder Fermentation raw material can produce more number of viable, promotes fermentation efficiency.
It is further preferred that the described compound probiotic for reproductive system also includes the raw material group of following parts by weight Point: 8～15 parts of Fructus Hordei Germinatus powder, the Flos Caryophylli of 15～30 parts and the Flos Lonicerae of 3～5 parts.
Bacillus pestis, vibrio cholera, anthrax bacillus, bacillus subtilis and staphylococcus aureus etc. are had by syringyl alcohol extractum Inhibitory action.The water of Flos Caryophylli, alcohol, ether leachate and volatile oil to pathogenic fungus all in obviously antibacterial action.Gold Flos Lonicerae the most just medical value with it is extensive and famous.Its effect is mainly heat-clearing and toxic substances removing, cures mainly epidemic febrile disease heating, pyretic toxicity Dysentery, carbuncle furunculosis etc..Modern study proves, Flos Lonicerae contains the pharmacological component such as chlorogenic acid, luteolin glycosides, to haemolysis The property various pathogens such as streptococcus, staphylococcus aureus and upper respiratory tract infection Causative virus etc. have stronger restraint, additionally Also can enhancing immunity, antiearly pregnancy, hepatoprotective, antitumor, antiinflammatory, antipyretic, hemostasis (blood coagulation), suppression intestinal absorption cholesterol etc., Widely, it is compatible for treating the diseases such as acute urinary system, genital system infection with present component for its clinical application Disease, can improve the immunity of reproductive system simultaneously.
Further, the described compound probiotic for reproductive system also includes the Fructus Zanthoxyli that parts by weight are 0.1～2 part Shell.
Fructus Zanthoxyli shell, i.e. Fructus Zanthoxyli, remove Fructus Zanthoxyli mesh (Fructus Zanthoxyli kind), Fructus Zanthoxyli shell, and it only has warming middle-JIAO to relieve pain, parasite killing The effect itched.
It is further preferred that the described compound probiotic for reproductive system also includes that parts by weight are 0.5～0.8 part Fructus Zanthoxyli shell.
Further, the described compound probiotic for reproductive system also includes the raw material components of following parts by weight: 1～ The Herba Andrographis of 20 parts, the Cortex Phellodendri of 1～20 part, the Fructus Schisandrae Chinensis of 1～20 part, the Fructus Crataegi of 1～20 part, the Radix Pulsatillae of 1～20 part, 1～ More than one in the glucose of the Semen Tritici aestivi fiber of 20 parts and 0.1～5 part.
Further, described Lactobacillus plantarum is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms Center, preservation date is on November 30th, 2015, and preserving number is CGMCC No.11763；
Described viable bacteria content in the compound probiotic of reproductive system is 7 × 1011～9 × 1011cfu/g。
The preparation method of a kind of above-mentioned compound probiotic for reproductive system, comprises the following steps: take soybean fiber powder Add water after being broken into granule, after soaking process, be prepared as material storage liquid, stirring, add sucrose, continuation stirring, quiet putting are stirred again Mixing, add ferment powder, stirring, quiet putting are again stirring for, and rise after warming the meridian aerobic fermentation processes and obtain aerobic fermentation treatment fluid, to described Adding Lactobacillus plantarum in aerobic fermentation treatment fluid, after stirring, sealing and fermenting obtains the compound probiotic for reproductive system.
Further, described stock solution storing solution also includes Fructus Hordei Germinatus powder, Flos Caryophylli and Flos Lonicerae, take Fructus Hordei Germinatus powder, Flos Caryophylli and Flos Lonicerae powder is broken into granule, is together added to the water with the pulverized particles of soybean fiber, makes material storage liquid after soaking process.
Further, in material storage liquid, glucose is also added when adding sucrose；
Described stock solution storing solution also includes in Herba Andrographis, Cortex Phellodendri, Fructus Schisandrae Chinensis, Fructus Crataegi, the Radix Pulsatillae and Semen Tritici aestivi fiber More than one；More than one taken in Herba Andrographis, Cortex Phellodendri, Fructus Schisandrae Chinensis, Fructus Crataegi, the Radix Pulsatillae and Semen Tritici aestivi fiber are ground into granule, with The pulverized particles of soybean fiber is together added to the water, and makes material storage liquid after soaking process；
The breaking method of described Fructus Hordei Germinatus powder, soybean fiber and Semen Tritici aestivi fiber is 1～2 μm for being crushed to particle diameter after drying； The breaking method of described Flos Lonicerae, Flos Caryophylli, Herba Andrographis, Cortex Phellodendri, Fructus Schisandrae Chinensis, Fructus Crataegi and the Radix Pulsatillae is in subzero 20～25 DEG C of frosts Being crushed to particle diameter after 40～60min immediately is 1～2 μm.
Further, after aerobic fermentation processes, first add the river of size-reduced one-tenth granule to described aerobic fermentation treatment fluid Green pepper shell, adds Lactobacillus plantarum after stirring, fermentation process, stirring, sealing and fermenting obtain the compound probiotic for reproductive system； The breaking method of described Fructus Zanthoxyli shell is 1～2 μm for being crushed to particle diameter immediately after freezing 40～60min in subzero 20～25 DEG C.
The preparation method feature based on each material composition that the present invention provides is designed, and it has step of preparation process and closes Reason, the compound probiotic for reproductive system of preparation keeps healthy, bactericidal effect is good, and the advantage that preparation cost is low.Aerobic fermentation The rear Fructus Zanthoxyli shell that adds carries out producing after fermentation can make the compatibility of Fructus Zanthoxyli shell effect of maximum, and effect retains perfect, its external treatment eczema, Pudendal pruritus, parasite killing, effect such as pain relieving, it is possible to be completely dissolved in cultivation bacterium, rear add Lactobacillus plantarum sealing and fermenting, with at utmost Turn out viable bacteria amount.
A kind of above-mentioned compound probiotic for reproductive system is preparing lubricant, reproductive system cleanout fluid, reproductive system Application in nursing gel, reproductive system nursing spray or liquid detergent.
Use technique scheme, including following beneficial effect: a kind of being combined for reproductive system that the present invention provides Probiotic bacteria and its production and use, its each material composition compatibility of the compound probiotic for reproductive system of the present invention closes Microecological balance in reason, effectively regulation men and women's reproductive system, in addition to this it is possible to produce nutrient substance, resists bacterial virus Infection and the function of some disease of prophylactic treatment, contribute to the self-regeneration in reproductive system, activation, maintenance, clear up, arrange Subtract deposit and strengthen reproductive system immunity etc..The preparation method of the present invention combines each material combination composition and determines, simultaneously Appropriate design fermentation step, makes the finished product of preparation become apparent from for reproductive system nursing, health-care effect.The present invention is used for system genitale The compound probiotic of system may be used for or be added on condom, human body lubricants, secret washing liquid, gel, spray, liquid detergent, with And part is treated and the nursing phallic product of sex.
Detailed description of the invention
Below by specific embodiment, the present invention is described in further detail.
Embodiment one: a kind of compound probiotic for reproductive system, including the raw material components of following parts by weight: 5 parts Lactobacillus plantarum powder, the ferment powder of 10 parts, the soybean fiber of 1 part and the sucrose of 5 parts.
The compound probiotic of the present invention also includes that conventional use of solvent, described solvent are preferably water, art technology Personnel can determine solvent species of the present invention and content value based on limited number of time experiment.
Embodiment two: a kind of compound probiotic for reproductive system, including the raw material components of following parts by weight: 10 parts Lactobacillus plantarum powder, the ferment powder of 8 parts, the soybean fiber of 2 parts and the sucrose of 3 parts.
The preparation method of a kind of compound probiotic for reproductive system, comprises the following steps: takes soybean fiber and is ground into Add water after granule, make water raw material components not soaked, after soaking process, be prepared as material storage liquid, stirring, add Entering sucrose, continuation stirring, quiet putting are stirred for, and add ferment powder, and stirring, quiet putting are again stirring for, after rising warming the meridian aerobic fermentation process Obtaining aerobic fermentation treatment fluid, add Lactobacillus plantarum in described aerobic fermentation treatment fluid, after stirring, sealing and fermenting is used Compound probiotic in reproductive system.
Owing to water is the preparation conventional use of solvent of compound probiotic of the present invention, its using dosage is based on limited number of time experiment all Available.
Described viable bacteria content in the compound probiotic of reproductive system is 7 × 1011cfu/g。
Embodiment three: a kind of compound probiotic for reproductive system, including the raw material components of following parts by weight: 12 parts Lactobacillus plantarum powder, the ferment powder of 5 parts, the soybean fiber of 4 parts, the sucrose of 2 parts, 2 parts of Fructus Hordei Germinatus powder, Flos Caryophyllis of 50 parts and 0.5 The Flos Lonicerae of part.
The preparation method of a kind of compound probiotic for reproductive system, comprises the following steps: take soybean fiber, Fructus Hordei Germinatus Powder, Flos Caryophylli and Flos Lonicerae powder add water after being broken into granule, are prepared as material storage liquid, described material storage liquid after soaking process Interior solid material is 0.05～2: 1 stirrings with the volume ratio of water, adds sucrose, and continuation stirring, quiet putting are stirred for, and add ferment Powder, stirring, quiet putting are again stirring for, and rise after warming the meridian aerobic fermentation processes and obtain aerobic fermentation treatment fluid, at described aerobic fermentation Adding Lactobacillus plantarum in reason liquid, after stirring, sealing and fermenting obtains the compound probiotic for reproductive system.
Embodiment four: a kind of compound probiotic for reproductive system, including the raw material components of following parts by weight: 50 parts Lactobacillus plantarum powder, the ferment powder of 1 part, the soybean fiber of 20 parts, the sucrose of 0.5 part, 30 parts of Fructus Hordei Germinatus powder, the Flos Caryophylli of 10 parts, The Flos Lonicerae of 10 parts, the Fructus Zanthoxyli shell of 0.1 part.
The compound probiotic of the present invention also includes that conventional use of solvent, described solvent are preferably water, art technology Personnel can determine solvent species of the present invention and content value based on limited number of time experiment.
The preparation method of a kind of compound probiotic for reproductive system, comprises the following steps:
Step one: raw material pulverizing processes: taking Fructus Hordei Germinatus powder and soybean fiber, being crushed to particle diameter after drying is 1～2 μm；Take It is 1～2 μm that Flos Lonicerae, Flos Caryophylli and Fructus Zanthoxyli shell are crushed to particle diameter after freezing 40～60min in subzero 20～25 DEG C immediately；
Step 2: preparation aerobic fermentation treatment fluid: take step once the soybean fiber of pulverization process, Fructus Hordei Germinatus powder, Flos Caryophylli and Flos Lonicerae powder adds water after being broken into granule, makes water be prepared as material storage liquid after not having each soaking process of particulate material component, Stirring, adds sucrose, and continuation stirring, quiet putting are stirred for, and add ferment powder, and stirring, quiet putting are again stirring for, and rise warming the meridian aerobic fermentation Aerobic fermentation treatment fluid is obtained after process；
Step 3: preparation is for the compound probiotic of reproductive system: add step one in described aerobic fermentation treatment fluid The Fructus Zanthoxyli shell of size-reduced one-tenth granule, adds Lactobacillus plantarum after stirring, fermentation process, stirring, sealing and fermenting obtain for reproduction The compound probiotic of system.
Described viable bacteria content in the compound probiotic of reproductive system is 9 × 1011cfu/g。
Embodiment five: a kind of compound probiotic for reproductive system, including the raw material components of following parts by weight: 18 parts Lactobacillus plantarum powder, the ferment powder of 3 parts, the soybean fiber of 5 parts, the sucrose of 3 parts, 15 parts of Fructus Hordei Germinatus powder, the Flos Caryophylli of 30 parts, 5 parts Flos Lonicerae and the Fructus Zanthoxyli shell of 0.8 part.
The compound probiotic of the present invention also includes that conventional use of solvent, described solvent are preferably water, art technology Personnel can determine solvent species of the present invention and content value based on limited number of time experiment.
The preparation method of a kind of compound probiotic for reproductive system, comprises the following steps:
Step one: raw material pulverizing processes: taking Fructus Hordei Germinatus powder and soybean fiber, being crushed to particle diameter after drying is 1～2 μm；Take It is 1～2 μm that Flos Lonicerae, Flos Caryophylli and Fructus Zanthoxyli shell are crushed to particle diameter after freezing 40～60min in subzero 20～25 DEG C immediately；
Step 2: preparation aerobic fermentation treatment fluid: take step once the soybean fiber of pulverization process, Fructus Hordei Germinatus powder, Flos Caryophylli and Flos Lonicerae powder adds water after being broken into granule, and soaking 6h is prepared as material storage liquid after processing, stir 8～10min, adds sugarcane Sugar, continues stirring 5min, the quiet 6h of putting and is stirred for 5min, adds ferment powder, and stirring 5min, the quiet 6h of putting are again stirring for 5min, heat up To 30 DEG C, every 6h stirs 5min, obtains aerobic fermentation treatment fluid after aerobic fermentation processes 10 days；
Step 3: preparation is for the compound probiotic of reproductive system: add step one in described aerobic fermentation treatment fluid The Fructus Zanthoxyli shell of size-reduced one-tenth granule, adds Lactobacillus plantarum after stirring 5min, fermentation process 24h, stirs 5min, sealing and fermenting 15 It obtains the compound probiotic for reproductive system.
Wherein Lactobacillus plantarum powder prepares according to a conventional method with Lactobacillus plantarum CGMCC No.11763.
Viable bacteria content in the compound probiotic for reproductive system of above-mentioned preparation is 8.75 × 1011cfu/g。
Embodiment six: a kind of compound probiotic for reproductive system, including the raw material components of following parts by weight: 10 parts Lactobacillus plantarum powder, the ferment powder of 2 parts, the soybean fiber of 2 parts, the sucrose of 2 parts, 8 parts of Fructus Hordei Germinatus powder, the Flos Caryophylli of 15 parts, 3 parts Flos Lonicerae and the Fructus Zanthoxyli shell of 0.5 part.
The compound probiotic of the present invention also includes that conventional use of solvent, described solvent are preferably water, art technology Personnel can determine solvent species of the present invention and content value based on limited number of time experiment.
The preparation method of a kind of compound probiotic for reproductive system, comprises the following steps:
Step one: raw material pulverizing processes: taking Fructus Hordei Germinatus powder and soybean fiber, being crushed to particle diameter after drying is 1～2 μm；Take It is 1～2 μm that Flos Lonicerae, Flos Caryophylli and Fructus Zanthoxyli shell are crushed to particle diameter after freezing 40～60min in subzero 20～25 DEG C immediately；
Step 2: preparation aerobic fermentation treatment fluid: take step once the soybean fiber of pulverization process, Fructus Hordei Germinatus powder, Flos Caryophylli and Flos Lonicerae powder adds water after being broken into granule, and soaking 6h is prepared as material storage liquid after processing, stir 8～10min, adds sugarcane Sugar, continues stirring 5min, the quiet 6h of putting and is stirred for 5min, adds ferment powder, and stirring 5min, the quiet 6h of putting are again stirring for 5min, heat up To 30 DEG C, every 6h stirs 5min, obtains aerobic fermentation treatment fluid after aerobic fermentation processes 10 days；
Step 3: preparation is for the compound probiotic of reproductive system: add step one in described aerobic fermentation treatment fluid The Fructus Zanthoxyli shell of size-reduced one-tenth granule, adds Lactobacillus plantarum after stirring 5min, fermentation process 24h, stirs 5min, sealing and fermenting 15 It obtains the compound probiotic for reproductive system.Wherein Lactobacillus plantarum powder be with Lactobacillus plantarum CGMCC No.11763 by Conventional method prepares.
Viable bacteria content in the compound probiotic for reproductive system of above-mentioned preparation is 7.12 × 1011cfu/g。
Embodiment seven: a kind of compound probiotic for reproductive system, including the raw material components of following parts by weight: 10 parts Lactobacillus plantarum powder, the ferment powder of 2 parts, the soybean fiber of 2 parts, the sucrose of 2 parts, the Herba Andrographis of 4 parts, the Cortex Phellodendri of 20 parts, 1 part Fructus Schisandrae Chinensis, the Fructus Crataegi of 15 parts and the Radix Pulsatillae of 5 parts.
The compound probiotic of the present invention also includes that conventional use of solvent, described solvent are preferably water, art technology Personnel can determine solvent species of the present invention and content value based on limited number of time experiment.
The preparation method of a kind of compound probiotic for reproductive system, comprises the following steps:
Step one: raw material pulverizing processes: taking Fructus Hordei Germinatus powder and soybean fiber, being crushed to particle diameter after drying is 1～2 μm；Take Flos Lonicerae, Flos Caryophylli, Fructus Zanthoxyli shell, Herba Andrographis, Cortex Phellodendri, Fructus Schisandrae Chinensis, Fructus Crataegi and the Radix Pulsatillae freeze 40～60min in subzero 20～25 DEG C After to be crushed to particle diameter immediately be 1～2 μm；
Step 2: preparation aerobic fermentation treatment fluid: take step once the soybean fiber of pulverization process, Fructus Hordei Germinatus powder, Flos Caryophylli, gold Flos Lonicerae, Herba Andrographis, Cortex Phellodendri, Fructus Schisandrae Chinensis, Fructus Crataegi and the Radix Pulsatillae add water after being ground into granule, and soaking 6h is prepared as former after processing Material storing solution, stirs 8～10min, adds sucrose, continues stirring 5min, the quiet 6h of putting and is stirred for 5min, adds ferment powder, stirring 5min, the quiet 6h of putting are again stirring for 5min, are warming up to 30 DEG C, and every 6h stirs 5min, obtain aerobic after aerobic fermentation processes 10 days Fermentation treatment fluid；
Step 3: preparation is for the compound probiotic of reproductive system: add step one in described aerobic fermentation treatment fluid The Fructus Zanthoxyli shell of size-reduced one-tenth granule, adds Lactobacillus plantarum after stirring 5min, fermentation process 24h, stirs 5min, sealing and fermenting 15 It obtains the compound probiotic for reproductive system.Wherein Lactobacillus plantarum powder be with Lactobacillus plantarum CGMCC No.11763 by Conventional method prepares.
Viable bacteria content in the compound probiotic for reproductive system of above-mentioned preparation is 7.01 × 1011cfu/g。
Embodiment eight: a kind of compound probiotic for reproductive system, including the raw material components of following parts by weight: 18 parts Lactobacillus plantarum powder, the ferment powder of 3 parts, the soybean fiber of 5 parts, the sucrose of 3 parts, 15 parts of Fructus Hordei Germinatus powder, the Flos Caryophylli of 30 parts, 5 parts Flos Lonicerae, the Fructus Zanthoxyli shell of 0.8 part, the Herba Andrographis of 10 parts, the Cortex Phellodendri of 8 parts, the Fructus Schisandrae Chinensis of 6 parts, the Fructus Crataegi of 10 parts, 10 parts white Head father-in-law, the Semen Tritici aestivi fiber of 10 parts and the glucose of 1 part.
The compound probiotic of the present invention also includes that conventional use of solvent, described solvent are preferably water, art technology Personnel can determine solvent species of the present invention and content value based on limited number of time experiment.
The preparation method of a kind of compound probiotic for reproductive system, comprises the following steps:
Step one: raw material pulverizing processes: taking Fructus Hordei Germinatus powder, soybean fiber and Semen Tritici aestivi fiber, being crushed to particle diameter after drying is 1 ～2 μm；Extracting honeysuckle, Flos Caryophylli, Fructus Zanthoxyli shell, Herba Andrographis, Cortex Phellodendri, Fructus Schisandrae Chinensis, Fructus Crataegi and the Radix Pulsatillae are in subzero 20～25 DEG C of frosts Being crushed to particle diameter after 40～60min immediately is 1～2 μm；
Step 2: preparation aerobic fermentation treatment fluid: take step fine once the soybean fiber of pulverization process, Fructus Hordei Germinatus powder, Semen Tritici aestivi Dimension, Flos Caryophylli, Flos Lonicerae, Herba Andrographis, Cortex Phellodendri, Fructus Schisandrae Chinensis, Fructus Crataegi and the Radix Pulsatillae add water, soaking 6h process after being ground into granule After be prepared as material storage liquid, stir 8～10min, add sucrose and glucose, continue stirring 5min, the quiet 6h of putting and be stirred for 5min, adds ferment powder, and stirring 5min, the quiet 6h of putting are again stirring for 5min, are warming up to 30 DEG C, and every 6h stirs 5min, sends out through aerobic Ferment obtains aerobic fermentation treatment fluid after processing 10 days；
Step 3: preparation is for the compound probiotic of reproductive system: add step one in described aerobic fermentation treatment fluid The Fructus Zanthoxyli shell of size-reduced one-tenth granule, adds Lactobacillus plantarum after stirring 5min, fermentation process 24h, stirs 5min, sealing and fermenting 15 It obtains the compound probiotic for reproductive system.Wherein Lactobacillus plantarum powder be with Lactobacillus plantarum CGMCC No.11763 by Conventional method prepares.
Viable bacteria content in the compound probiotic for reproductive system of above-mentioned preparation is 9 × 1011cfu/g。
Embodiment nine: a kind of compound probiotic for reproductive system, including the raw material components of following parts by weight: 18 parts Lactobacillus plantarum powder, the ferment powder of 3 parts, the soybean fiber of 5 parts, the sucrose of 3 parts, 15 parts of Fructus Hordei Germinatus powder, the Flos Caryophylli of 30 parts, 5 parts Flos Lonicerae, the Fructus Zanthoxyli shell of 0.8 part, the Herba Andrographis of 1 part, the Fructus Crataegi of 20 parts, the Radix Pulsatillae of 1 part, the Semen Tritici aestivi fiber of 20 parts and 0.1 The glucose of part.
The compound probiotic of the present invention also includes that conventional use of solvent, described solvent are preferably water, art technology Personnel can determine solvent species of the present invention and content value based on limited number of time experiment.
The preparation method of a kind of compound probiotic for reproductive system, comprises the following steps:
Step one: raw material pulverizing processes: taking Fructus Hordei Germinatus powder, soybean fiber and Semen Tritici aestivi fiber, being crushed to particle diameter after drying is 1 ～2 μm；Extracting honeysuckle, Flos Caryophylli, Fructus Zanthoxyli shell, Herba Andrographis, Fructus Crataegi and the Radix Pulsatillae are vertical after freezing 40～60min in subzero 20～25 DEG C I.e. being crushed to particle diameter is 1～2 μm；
Step 2: preparation aerobic fermentation treatment fluid: take step fine once the soybean fiber of pulverization process, Fructus Hordei Germinatus powder, Semen Tritici aestivi Dimension, Flos Caryophylli, Flos Lonicerae, Herba Andrographis, Fructus Crataegi and the Radix Pulsatillae add water after being ground into granule, and soaking 6h is prepared as raw material after processing Storing solution, stirs 8～10min, adds sucrose and glucose, continues stirring 5min, the quiet 6h of putting and is stirred for 5min, adds ferment Powder, stirs 5min, the quiet 6h of putting and is again stirring for 5min, be warming up to 30 DEG C, and every 6h stirs 5min, after aerobic fermentation processes 10 days To aerobic fermentation treatment fluid；
Step 3: preparation is for the compound probiotic of reproductive system: add step one in described aerobic fermentation treatment fluid The Fructus Zanthoxyli shell of size-reduced one-tenth granule, adds Lactobacillus plantarum after stirring 5min, fermentation process 24h, stirs 5min, sealing and fermenting 15 It obtains the compound probiotic for reproductive system.Wherein Lactobacillus plantarum powder be with Lactobacillus plantarum CGMCC No.11763 by Conventional method prepares.
Viable bacteria content in the compound probiotic for reproductive system of above-mentioned preparation is 8.95 × 1011cfu/g。
Embodiment ten: a kind of compound probiotic for reproductive system, including the raw material components of following parts by weight: 18 parts Lactobacillus plantarum powder, the ferment powder of 3 parts, the soybean fiber of 5 parts, the sucrose of 3 parts, 15 parts of Fructus Hordei Germinatus powder, the Flos Caryophylli of 30 parts, 5 parts Flos Lonicerae, the Fructus Zanthoxyli shell of 0.8 part, the Herba Andrographis of 20 parts, the Cortex Phellodendri of 1 part, the Fructus Schisandrae Chinensis of 20 parts, the Fructus Crataegi of 1 part, 20 parts white Head father-in-law, the Semen Tritici aestivi fiber of 1 part and the glucose of 5 parts.
The compound probiotic of the present invention also includes that conventional use of solvent, described solvent are preferably water, art technology Personnel can determine solvent species of the present invention and content value based on limited number of time experiment.
The preparation method of a kind of compound probiotic for reproductive system, comprises the following steps:
Step one: raw material pulverizing processes: taking Fructus Hordei Germinatus powder, soybean fiber and Semen Tritici aestivi fiber, being crushed to particle diameter after drying is 1 ～2 μm；Extracting honeysuckle, Flos Caryophylli, Fructus Zanthoxyli shell, Herba Andrographis, Cortex Phellodendri, Fructus Schisandrae Chinensis, Fructus Crataegi and the Radix Pulsatillae are in subzero 20～25 DEG C of frosts Being crushed to particle diameter after 40～60min immediately is 1～2 μm；
Step 2: preparation aerobic fermentation treatment fluid: take step fine once the soybean fiber of pulverization process, Fructus Hordei Germinatus powder, Semen Tritici aestivi Dimension, Flos Caryophylli, Flos Lonicerae, Herba Andrographis, Cortex Phellodendri, Fructus Schisandrae Chinensis, Fructus Crataegi and the Radix Pulsatillae add water, soaking 6h process after being ground into granule After be prepared as material storage liquid, stir 8～10min, add sucrose and glucose, continue stirring 5min, the quiet 6h of putting and be stirred for 5min, adds ferment powder, and stirring 5min, the quiet 6h of putting are again stirring for 5min, are warming up to 30 DEG C, and every 6h stirs 5min, sends out through aerobic Ferment obtains aerobic fermentation treatment fluid after processing 10 days；
Step 3: preparation is for the compound probiotic of reproductive system: add step one in described aerobic fermentation treatment fluid The Fructus Zanthoxyli shell of size-reduced one-tenth granule, adds Lactobacillus plantarum after stirring 5min, fermentation process 24h, stirs 5min, sealing and fermenting 15 It obtains the compound probiotic for reproductive system.Wherein Lactobacillus plantarum powder be with Lactobacillus plantarum CGMCC No.11763 by Conventional method prepares.
Viable bacteria content in the compound probiotic for reproductive system of above-mentioned preparation is 8.59 × 1011cfu/g。
The present invention is for the compound probiotic In vitro Bactericidal Experiments of reproductive system:
Test strain:
Select staphylococcus aureus, Candida albicans, escherichia coli, staphylococcus epidermidis and trichomonas vaginitis.
Bacterium culture medium: nutrient agar and nutrient broth medium.
Instrument and reagent:
Biochemical cultivation case, model is SPH-150, Shanghai Sen Xin test apparatus company limited
Colonometer, model is BOT-50A, Beijing China National Instruments Import & Export Corporation Bo Teng Science and Technology Ltd.
AE-240 electronic analytical balance, prunus mume (sieb.) sieb.et zucc. Teller-Tuo Li Instrument Ltd.
UV-2401 type spectrophotometer, Japan's Shimadzu
Test packet:
Bacteriostatic test separating tests group and matched group, test 1 group to 8 groups select respectively described in embodiment 1 to embodiment 8 answer Closing probiotic bacteria, compareing 1 group and selecting Chinese patent application publication No. is the ecological gynaecology external-use drug disclosed in CN 105,663 172 A Compositions, compares 2 groups and selects hypochlorous acid disinfectant solution.
Content of the test: by the streak inoculation respectively of above-mentioned four kinds of strains and trichomonas vaginitis on five agar culture mediums, 37 DEG C After cultivating 24h, isolating single bacterium colony, switching, with broth bouillon, is placed in 37 DEG C of calorstats cultivation 24h.Take 1ml to cultivate Liquid, uses normal saline to be diluted successively with 10 times of dilution methods, and the 0.1ml bacterium solution choosing debita spissitudo is placed in the training of plate agar Support on base, smoothen, 37 DEG C of constant temperature culture 48h, calculate clump count.The viable bacteria of every milliliter of bacterium solution is calculated according to bacterium solution diluted concentration Number, is diluted to 106/ml, as test strain by bacterium solution.
Take each 10g of compositions described in the embodiment of the present invention 1 to embodiment 8；Add 20ml and melt the fine jade being cooled to 50-60 DEG C Fat culture medium, mixing, sucking-off 10ml, in another plate, adds 10ml agar culture medium in remaining 10ml pastille plate, mix, Sucking-off 10ml is in another plate again, repeat the above steps, to make pastille substrate concentration be 500,250,125,62.5,31.25, 15.6、7.8、3.9mg/ml；And take Chinese patent application and be published as the ecological gynaecology external-use drug disclosed in CN 105,663 172 A Compositions, according to above-mentioned steps, to make pastille substrate concentration be 500,250,125,62.5,31.25,15.6,7.8,3.9mg/ ml；Hypochlorous acid disinfectant solution 6mL, adds the agar culture medium being cooled to 45-50 DEG C after 34ml melts, and mixing, by the invention described above The dilution process of drug plate, be the plate ultimate density containing hypochlorous acid disinfectant solution be 450,225,112,56,28,14,7, 2,1,0.5ug/ml, standby.
Taking aforementioned standby bacterium solution, every vaccination inoculation 2ul bacterium solution, on corresponding pastille plate, inoculates corresponding time simultaneously Chloric acid disinfectant solution culture medium, as a control group 1, inoculate not in pastille culture medium as a control group 2, cultivate 48 hours at 37 DEG C Observed result.
Using plate sesquialter incorporation methods to measure the external to antibacterial half Mlc (MIC50) of compositions, data process Use Bliss to analyze software, the results are shown in Table 1.
Table 1 compositions is in vitro to antibacterial half Mlc (crude drug mg/ml)
"+", indicates that bacteria growing, embodiment have significant difference (P compared with comparison 1 groupa＜ 0.01)
Conclusion: from above-mentioned test, the compound probiotic for reproductive system provided by the present invention is to golden yellow Portugal Grape coccus, Candida albicans, escherichia coli, staphylococcus epidermidis and trichomonas vaginitis all have significant inhibitory action, except epidermis Portugal Outside grape coccus, the antibacterial action of embodiment five and embodiment eight is the most excellent compared with other embodiments, the antibacterial activity phase of two embodiments Significant difference is respectively provided with for matched group.
Compositions is to mouse vagina mucomembranous irritant test:
Animal subject:
Adult healthy female sd inbred rats 40,180-220g.
Test packet:
It is 13 groups by 65 female sd inbred rats random assortment, often group 5, test 1 group and select enforcement respectively to test 10 groups Compositions described in example 1 to embodiment 10, compareing 1 group and selecting Chinese patent application publication No. is disclosed in CN 105663172 A Ecological gynecology externally used pharmaceutical combination, section's washing liquid, compareing 2 groups is normal saline group；Compare 3 groups for blank group.
Content of the test:
The SD rat of test group and comparison 1 group is soaked with Chinese medicine composition aqueous solution with appropriately sized sterilizing absorbent cotton, puts In SD rat vagina, compare 2 groups and be placed in tested SD rat vagina with onesize cotton sliver leaching sterile saline, all with dynamic Thing vaginal mucosa contacts 24 hours, and blank 3 groups does not the most give any process, puts to death animal, take out whole the moon after 24 hours Road, observes the hyperemia of topical vaginal tissue, edema reacting phenomenon, carries out irritant reaction evaluation.
Result of the test:
Test group and matched group SD rat topical vaginal tissue all do not find the abnormal response such as hyperemia, edema, do not find yet Other symptoms, vaginal mucosa irritation index+0.0；Result shows, the compound probiotic pair for reproductive system of the present invention The vaginal mucosa irritation of female sd inbred rats belongs to nonirritant.
Clinical trial:
Diagnostic criteria: scorching according to the vulvitis described in " Chinese and western medical science diagnostics ", colpitis, cervicitis, suitcase and The symptom of balanitis, and Clinical detection result.
Test case:
1, suitcase inflammation patient 195 example, the age, all between 20-55, distribution 1 group of 66 example for the treatment of, treats 2 group of 65 example, comparison Organize 64 examples.
2, balanitis patient 160 example, the age, all between 20-55, distribution 1 group of 54 example for the treatment of, treats 2 group of 53 example, comparison Organize 52 examples.
3, colpitis mycotica patient 108 example, all age 18-60 year between, distribution treatment 1 group, treat 2 groups, comparison Organize each 36 examples.
4, bacterial vaginitis patient 83 example, all age 18-62 year between, distribution 1 group of 28 example for the treatment of, treat 2 group 28 Example, matched group 27 example.
5, trichomonal vaginitis patient 97 example, the age all between 20-55, distribution 1 group of 33 example for the treatment of, treat 2 group of 32 example, Matched group 32 example.
6, virus in patients with cervicitis 100 example, all age 22-65 year between, distribution 1 group of 33 example for the treatment of, treat 2 group of 34 example, right According to organizing 33 examples.
Efficacy assessment standard
With clinical symptoms with local sign as criterion
Recovery from illness: clinical symptoms disappears with local sign, reaches clinical cure standard；
Effective: after treatment, state of an illness sign is obviously improved；
Effective: after treatment, state of an illness sign makes moderate progress；
Invalid: unchanged before and after treatment.
Packet and administration:
More than treat the external preparation that 1 group of compositions all given described in the embodiment of the present invention 5 is made, treat 2 groups all to Giving the external preparation that the compositions described in the embodiment of the present invention 8 is made, matched group gives commercially available conventional reproductive system nursing medicinal Thing external preparation, uses 1.0g (crude drug amount) every day, uses 20 days continuously.Therapeutic effect is shown in Table 2.
Table 2 clinical trial results
From the data in table 2, it can be seen that be far superior to matched group for most preferred embodiment five and its therapeutic effect of embodiment eight, The embodiment of the present invention is for suitcase inflammation, balanitis, colpitis mycotica, bacterial vaginitis, trichomonal vaginitis and cervicitis All there are preferably treatment and nursing role.The foregoing is only the preferred embodiments of the present invention, be not limited to this Bright, for a person skilled in the art, the present invention can have various modifications and variations.All in the spirit and principles in the present invention Within, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.

Claims (10)

1. the compound probiotic for reproductive system, it is characterised in that include the raw material components of following parts by weight: 5～ The Lactobacillus plantarum powder of 50 parts, the ferment powder of 1～10 part, the soybean fiber of 1～20 part and the sucrose of 0.5～5 part.
Compound probiotic for reproductive system the most according to claim 1, it is characterised in that described for reproductive system Compound probiotic also include the raw material components of following parts by weight: 2～30 parts of Fructus Hordei Germinatus powder, the Flos Caryophylli of 10～50 parts and 0.5～ The Flos Lonicerae of 10 parts.
Compound probiotic for reproductive system the most according to claim 1, it is characterised in that described for reproductive system Compound probiotic also include the Fructus Zanthoxyli shell that parts by weight are 0.1～2 part.
Compound probiotic for reproductive system the most according to claim 1, it is characterised in that described for reproductive system Compound probiotic also include the raw material components of following parts by weight: the Herba Andrographis of 1～20 part, the Cortex Phellodendri of 1～20 part, 1～20 Fructus Schisandrae Chinensis, the Fructus Crataegi of 1～20 part, the Radix Pulsatillae of 1～20 part, the Semen Tritici aestivi fiber of 1～20 part and the glucose of 0.1～5 part of part In more than one.
5. according to the compound probiotic for reproductive system described in any one of Claims 1 to 4, it is characterised in that described Lactobacillus plantarum is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preservation date is 2015 11 The moon 30, preserving number is CGMCC No.11763；
Described viable bacteria content in the compound probiotic of reproductive system is 7 × 1011～9 × 1011cfu/g。
6. a preparation method for the compound probiotic for reproductive system described in any one of Claims 1 to 5, its feature exists In, comprise the following steps: take after soybean fiber is ground into granule and add water, be prepared as material storage liquid after soaking process, stir Mixing, add sucrose, continuation stirring, quiet putting are stirred for, and add ferment powder, and stirring, quiet putting are again stirring for, and rise at warming the meridian aerobic fermentation Obtaining aerobic fermentation treatment fluid after reason, add Lactobacillus plantarum in described aerobic fermentation treatment fluid, after stirring, sealing and fermenting obtains To the compound probiotic for reproductive system.
The preparation method of the compound probiotic for reproductive system the most according to claim 6, it is characterised in that described Stock solution storing solution also includes Fructus Hordei Germinatus powder, Flos Caryophylli and Flos Lonicerae, takes Fructus Hordei Germinatus powder, Flos Caryophylli and Flos Lonicerae powder and be broken into granule, with Semen sojae atricolor The pulverized particles of fiber is together added to the water, and makes material storage liquid after soaking process.
The preparation method of the compound probiotic for reproductive system the most according to claim 7, it is characterised in that adding In material storage liquid, glucose is also added during sucrose；
Described stock solution storing solution also includes the one in Herba Andrographis, Cortex Phellodendri, Fructus Schisandrae Chinensis, Fructus Crataegi, the Radix Pulsatillae and Semen Tritici aestivi fiber Above；More than one taken in Herba Andrographis, Cortex Phellodendri, Fructus Schisandrae Chinensis, Fructus Crataegi, the Radix Pulsatillae and Semen Tritici aestivi fiber are ground into granule, with Semen sojae atricolor The pulverized particles of fiber is together added to the water, and makes material storage liquid after soaking process；
The breaking method of described Fructus Hordei Germinatus powder, soybean fiber and Semen Tritici aestivi fiber is 1～2 μm for being crushed to particle diameter after drying；Described The breaking method of Flos Lonicerae, Flos Caryophylli, Herba Andrographis, Cortex Phellodendri, Fructus Schisandrae Chinensis, Fructus Crataegi and the Radix Pulsatillae be in subzero 20～25 DEG C freeze 40～ Being crushed to particle diameter after 60min immediately is 1～2 μm.
9., according to the preparation method of the compound probiotic for reproductive system described in any one of claim 6～8, its feature exists In, after aerobic fermentation processes, first add the Fructus Zanthoxyli shell of size-reduced one-tenth granule to described aerobic fermentation treatment fluid, stir, ferment Adding Lactobacillus plantarum after process, stirring, sealing and fermenting obtain the compound probiotic for reproductive system；The powder of described Fructus Zanthoxyli shell Broken method is 1～2 μm for being crushed to particle diameter immediately after freezing 40～60min in subzero 20～25 DEG C.
10. the compound probiotic for reproductive system described in an any one of Claims 1 to 5 is preparing lubricant, reproduction Application in cleaning systems liquid, reproductive system nursing gel, reproductive system nursing spray or liquid detergent.
CN201610768383.6A 2016-08-30 2016-08-30 A kind of compound probiotic for reproductive system and its production and use Pending CN106265864A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1465358A (en) * 2002-06-21 2004-01-07 陈必诚 Vagina girdle, clean and anti-inflamation agent for female
CN102670917A (en) * 2012-01-20 2012-09-19 华子昂 Composite enzyme and its preparation method
CN103409334A (en) * 2013-01-16 2013-11-27 生合生物科技股份有限公司 Lactobacillus for inhibiting pathogenic bacteria of vaginitis and application thereof
CN104399017A (en) * 2014-10-27 2015-03-11 韩国英 Composition for prevention and treatment of female vulva inflammation
CN105263504A (en) * 2013-05-14 2016-01-20 益生菌股份公司 Composition comprising lactic acid bacteria for use in the preventive and/or curative treatment of bacterial vaginosis

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1465358A (en) * 2002-06-21 2004-01-07 陈必诚 Vagina girdle, clean and anti-inflamation agent for female
CN102670917A (en) * 2012-01-20 2012-09-19 华子昂 Composite enzyme and its preparation method
CN103409334A (en) * 2013-01-16 2013-11-27 生合生物科技股份有限公司 Lactobacillus for inhibiting pathogenic bacteria of vaginitis and application thereof
CN105263504A (en) * 2013-05-14 2016-01-20 益生菌股份公司 Composition comprising lactic acid bacteria for use in the preventive and/or curative treatment of bacterial vaginosis
CN104399017A (en) * 2014-10-27 2015-03-11 韩国英 Composition for prevention and treatment of female vulva inflammation

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