CN112472772A - Antibacterial gel and preparation method thereof - Google Patents

Antibacterial gel and preparation method thereof Download PDF

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CN112472772A
CN112472772A CN202011464940.8A CN202011464940A CN112472772A CN 112472772 A CN112472772 A CN 112472772A CN 202011464940 A CN202011464940 A CN 202011464940A CN 112472772 A CN112472772 A CN 112472772A
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traditional chinese
chinese medicine
fermentation
deionized water
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杜俊勇
杜立刚
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Jiangxi Changtong Industry Co ltd
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Jiangxi Changtong Industry Co ltd
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Abstract

The invention provides an antibacterial gel and a preparation method thereof, and relates to the technical field of medicines. The bacteriostatic gel comprises fermented traditional Chinese medicines subjected to fermentation catalysis of dicationic acidic ionic liquid and microbial agent, a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water. The method comprises the following steps: s1, weighing the traditional Chinese medicines according to the formula proportion, mixing, crushing and sterilizing; s2, adding a fermentation catalyst and deionized water for fermentation; s3, drying the fermented traditional Chinese medicine mixture, adding ethanol for extraction to obtain traditional Chinese medicine liquid, and removing the ethanol in the traditional Chinese medicine liquid to obtain fermented traditional Chinese medicine; s4, mixing the fermented traditional Chinese medicine with a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water to prepare the antibacterial gel. The vaginal microecological environment of patients with vaginitis is improved by inhibiting the growth of harmful bacteria and regulating the growth and reproduction of vaginal probiotics, and the disease relapse is reduced.

Description

Antibacterial gel and preparation method thereof
Technical Field
The invention relates to the technical field of medicines, and particularly relates to an antibacterial gel and a preparation method thereof.
[Ps2TMEDA][BF4]2The name of N, N ' -dipropylsulfonic acid group-N, N, N ', N ' -tetramethyl ethylenediamine tetrafluoroborate and the names of ionic liquids of other different anions are analogized in the same way;
[Bs2TMEDA][BF4]2the name of N, N ' -dibutyl sulfonic group-N, N, N ', N ' -tetramethyl ethylenediamine tetrafluoroborate, ionic liquids of other different anions and the like.
Background
Vaginal flora plays an irreplaceable role in maintaining the stability and regulation of the environment in the vagina. The vaginal flora is very complex and comprises many aerobic bacteria and anaerobic bacteria besides protozoa and fungi, such as streptococcus lactis, enterobacter, proteus, gargococcus, wiener and the like which are common at the lower end of the vagina, the microorganisms can be classified into commensal microorganisms and pathological microorganisms which grow in the common environment, and the microorganisms can have an orange resistance effect. The other one influencing the growth is hydrogen ion concentration, when the pH value is 3.8-4.2, the propagation of commensal probiotics, especially lactobacillus, which is the main probiotic strain in healthy vagina, and the density in vaginal fluid can reach 105～108ml, when the vagina is infected by pathogenic microorganism, if lactobacillus is dominant, weak acid environment with pH of 3.8-4.2 in the vagina can be maintained, the vagina can not be diseased, and importantly, the lactobacillus can also generate H202Inhibiting the reproduction of other microorganisms by having a toxic effect on themAnd (4) breeding. Once the reproductive capacity of pathogenic microorganisms exceeds that of probiotics, the growth environment of the probiotics can be damaged, the environment in the vagina is further damaged, and finally gynecological diseases are caused. After gynecological diseases occur, the regulation and treatment cannot be effectively performed, and intractable inflammation attacks repeatedly, which seriously affects the reproductive health of women.
Vaginitis is a common disease of obstetrics and gynecology, married women suffer from vaginitis for at least 1-2 times in the life, and pregnant and lying-in women suffer from vaginitis which affects the pregnancy result and the postpartum course. Generally, the vagina has some resident flora such as B-type hemolytic streptococcus, candida albicans and the like, and when the body resistance is low, the vaginal mucosa is injured or the acidity in the vagina is changed, the virulence of the bacteria is increased, and the bacteria become pathogenic bacteria to cause vaginitis to happen. For example, the glycogen content of vaginal epithelial cells of a pregnant woman is increased, the acidity of the vagina is enhanced, the renal threshold is reduced, the sugar content in urine is increased, and the growth and the reproduction of candida albicans are promoted. Clinically, vaginitis is classified into nonspecific, fungal, bacterial, viral and protozoal vaginitis according to the etiology of the vaginal inflammatory disease.
The current major treatment for the disease is by antibiotic or antiseptic treatment, killing all vaginal bacteria thoroughly to clean the vagina. The vagina after treatment often needs a long time for self-repair, and more serious people can lose the self-regulation capability of the vagina due to improper treatment. By consulting the existing products and patents, the gel products sold in the private parts and having the formal letters are mostly discovered to be prepared by adding quaternary ammonium cation as a bactericide in a sterilizing mode, such as: chlorhexidine acetate, benzalkonium chloride, etc. have the function of killing bacteria by changing the permeability of bacterial cytoplasmic membrane to make the bacterial cytoplasmic substance exosmose and block its metabolism. Such disinfectants have been reported to cause allergic conjunctivitis, vision loss, contact dermatitis, and the like, and even death in severe cases. The bactericide kills probiotics while killing harmful bacteria, so that the environment in the vagina is disrupted. In addition, some washing liquid needles can clean the vagina and vulva, but the washing liquid contains surfactant and is alkaline or neutral, the washing with alkaline bath cream or tap water is not scientific, and the weak acid environment of the vagina is easily damaged by frequent use of the washing liquid, the disinfection pad and the like. Long-term application of antibacterial drugs easily causes disease recurrence, and the growth of pathogenic bacteria is well controlled on the premise of protecting probiotics, and the roots of the human body are treated and maintained by means of the self-regulation capacity of the vagina.
Ionic Liquids (ILs) are salts which are composed of positive ions and negative ions and are liquid at room temperature or use temperature, and are green solvents and catalysts which have strong dissolving capacity, are non-volatile, have high thermal stability and can be designed. The ionic liquid has excellent performances in the aspects of catalytic preparation, extraction and separation, analysis and detection, physical property modification and the like of food components. Research shows that the anions and cations in the ionic liquid contain different functional groups such as hydroxyl and the like to form hydrogen bonds, destroy the crystalline structure of the microcrystalline dietary fiber and enhance the solubility of the dietary fiber. Therefore, the ionic liquid has great potential for preparing fermented traditional Chinese medicines.
Disclosure of Invention
The invention aims to solve the problems and provides a bacteriostatic gel which is used for improving the vaginal micro-ecological environment of a vaginitis patient and helping to reduce disease recurrence by regulating the growth and reproduction of vaginal probiotics and a preparation method thereof.
In one aspect of the object of the present invention, there is provided a bacteriostatic gel comprising: the traditional Chinese medicine fermentation product comprises traditional Chinese medicines fermented by the fermentation catalysis of dicationic acidic ionic liquid and microbial agent, probiotics, pH regulator, nutrition humectant, thickener, chelating agent and deionized water.
The biological activity and the bioavailability of natural products in the fermented traditional Chinese medicine are greatly enhanced, so that the medicinal health-care function of the fermented traditional Chinese medicine is improved, and the prepared antibacterial gel has very excellent curative effect on female vaginitis by combining the regulating effect of probiotics. The fermented traditional Chinese medicine contains various natural products, is scientific and reasonable in matching, can maintain acid-base balance in the vagina, has an inhibiting effect on haemophilus vaginalis, promotes proliferation of the lactobacilli vaginalis, enhances vaginal immunity and metabolism, enables the vagina to have self-cleaning capacity and defense capacity, can clean the inside of the vagina, can improve vaginal health and vaginal verification, is high in safety performance, and has no stimulation to skin.
The microbial agent can perform metabolic activity in the fermentation process to generate probiotics, and the probiotics can be effectively combined with various pathogenic bacteria and viruses to prevent the activities of the pathogenic bacteria and the viruses and even ensure the life apoptosis of the pathogenic bacteria and the viruses, so that the microbial agent has the functions of sterilization and antivirus. Therefore, the probiotics can generate synergistic effect with probiotics and fermented traditional Chinese medicines, and the drug effect of the antibacterial gel is greatly improved. Meanwhile, the substances are generated by metabolism of probiotics, and the probiotics are microorganisms which are naturally selected along with the evolution of human bodies, adapt to the human bodies and are beneficial to the human bodies, so the substances generated by the metabolism of the probiotics, namely the probiotics are safe for the human bodies.
The dicationic acidic ionic liquid in the formula is a cationic system, harmful bacteria at the vagina are generally negatively charged, when encountering cations, the cations can puncture the cell walls of the bacteria and enter the cells under the coulomb law, and the functions of inhibiting bacteria or killing the bacteria can be achieved through the synergistic effect of the antibacterial and bactericidal components in the formula.
According to the invention, the chelating agent is added into the formula of the chelating agent, so that the thixotropy of gel is enhanced, the gel can be deeply dispersed into the inner wall and the fold area, the garbage toxin retained in the folds is removed, a series of problems caused by dirt toxin deposited at the folds are solved, and the exertion of drug effect is promoted; can also play a synergistic role with the thickening agent, and reduce the using amount of the thickening agent.
Preferably, the dicationic acidic ionic liquid is sulfonic acid functionalized tetramethylethylenediamine ionic liquid.
The invention uses sulfonic acid functionalized tetramethylethylenediamine ionic liquid as a fermentation catalyst, has good biocompatibility and catalytic activity, can improve the fermentation environment in the fermentation process, assists and promotes microorganisms to destroy cell wall structures and extracellular polymers of biomass, reacts with a large amount of crystalline cellulose in traditional Chinese medicines, promotes the decrystallization of the cellulose, accelerates the conversion rate of cellulose sugar, and obtains the environmental condition more suitable for the growth of microorganisms. Meanwhile, the microbial inoculum is matched, and the two agents generate synergistic action, so that the fermentation efficiency is improved, the fermentation time is shortened, the gas generation is reduced, and the biological activity and the bioavailability of natural products in the fermented traditional Chinese medicine are improved. In addition, the ionic liquid can also be used as an enzyme keep-alive agent, so that the activity of a microbial agent is protected, and various extracellular ferments are complete and strong in activity. In addition, the ionic liquid has acidity similar to that of the vaginal environment, so that the acidity of the antibacterial gel is not influenced, and the use safety is ensured.
Preferably, the preparation method of the dicationic acidic ionic liquid comprises the following steps:
a. adding 1, 3-propane sultone or 1, 4-propane sultone into an ethanol solution of tetramethylethylenediamine under the condition of stirring, reacting for a period of time at 60-100 ℃, and separating the obtained solid intermediate product;
b. and (4) adding an acid solution into the solid intermediate product obtained in the step S1, stirring and reacting for a period of time at the temperature of 60-100 ℃, and removing the solvent and residual reactants in the reaction mixture to obtain the dicationic acidic ionic liquid.
Preferably, the microbial agent comprises one or more of yeast, acetobacter xylinum, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus bifidus. The microbial agent disclosed by the invention is beneficial bacteria, can adjust the clean and balanced environment in the vagina and achieves the purpose of daily maintenance of the vagina.
Lactobacillus is representative of probiotics in vagina, and Lactobacillus crispatus produces H2O2The vaginal Lactobacillus crispatus has the strongest capability, can competitively exclude the colonization of other bacteria in the vagina, and women taking the Lactobacillus crispatus as the dominant bacteria have lower proportion of the bacterial vaginosis.
Preferably, the traditional Chinese medicine comprises: 20-50 parts of oat, 20-50 parts of radix sophorae flavescentis, 20-50 parts of red peony root, 10-40 parts of eucalyptus globulus leaf, 10-40 parts of fructus cnidii, 10-40 parts of centella, 10-40 parts of teasel root, 10-30 parts of folium cortex eucommiae, 10-30 parts of guava leaf, 10-30 parts of lithospermum, 0-20 parts of borneol, 0-20 parts of angelica sinensis and 0-20 parts of lotus leaf.
Preferably, the bacteriostatic gel comprises the following raw materials in parts by weight: 2-10 parts of fermented traditional Chinese medicine, 1-5 parts of pH regulator, 2-10 parts of nutritional humectant, 1-5 parts of thickener, 1-5 parts of chelating agent and 50-100 parts of deionized water.
Oats are called as 'full-value nutritional food' by nutritionists, are rich in various functional components such as protein, fat, saponin alkaloid and the like, and can be released through fermentation catalysis. Modern pharmacological research shows that the oat alkaloid has important effects of strong oxidation resistance, antianaphylaxis, lipid reduction, antiproliferation, itching relieving, inflammation diminishing and the like, and is an excellent skin protectant. The avenanthramides are alkaline organic compounds with cyclic structures, are unique nitrogen-containing phenolic acid derivatives, are also the only nitrogen-containing organic compounds found in the oats, and have remarkable biological activity and optical activity. The avenanthramides have anti-inflammatory and antipruritic effects by inhibiting histamine signal transduction.
Kuh-seng has the functions of clearing heat and drying dampness, killing parasites and promoting urination. Can be used for treating dysentery with heat, hematochezia, jaundice, anuria, leucorrhea with red and white discharge, pudendal swelling, pudendal pruritus, eczema, skin pruritus, scabies, tinea, leprosy, and trichomonas vaginitis.
Radix Paeoniae Rubra has effects of clearing heat, cooling blood, removing blood stasis and relieving pain; can be used for treating macula due to toxic heat, hematemesis, epistaxis, conjunctival congestion, swelling and pain, liver depression, hypochondriac pain, amenorrhea, dysmenorrhea, abdominal pain, traumatic injury, carbuncle, swelling, and pyocutaneous disease.
The Eucalyptus globulus Labill leaf has the effects of dispelling wind and clearing heat, sterilizing and diminishing inflammation, and preventing corrosion and relieving itching. It can be used for treating common cold, influenza, dysentery, enteritis, arthralgia, cystitis, scald, scabies, erysipelas, neurodermatitis, eczema, carbuncle, sore, and toxic swelling.
Fructus Cnidii has effects of warming kidney, tonifying yang, eliminating dampness, dispelling pathogenic wind, and killing parasite. Can be used for treating sexual impotence, cold womb, leukorrhagia due to cold-dampness, lumbago due to damp arthralgia, vulval eczema, pudendal pruritus, and trichomonas vaginitis.
Centella asiatica has the effects of clearing heat, promoting diuresis, relieving swelling and removing toxicity. It is used for treating abdominal pain due to eruptive disease, summer diarrhea, dysentery, jaundice due to damp-heat pathogen, stranguria with blood, hematuria, emesis, epistaxis, hemoptysis, conjunctival congestion, larynx swelling, rubella, scabies, tinea, furuncle, carbuncle, toxic swelling, and traumatic injury.
Radix Dipsaci has antibacterial, antiinflammatory, immunity regulating, antioxidant, antiaging, bone injury healing promoting, and premature labor abortion resisting effects. Tonify liver and kidney, strengthen tendons and bones, heal fracture and stop metrorrhagia and metrostaxis. They belong to yang tonics classified under deficiency tonics.
The folium Eucommiae has tranquilizing, analgesic, blood pressure lowering, and antiinflammatory effects, and can enhance immunity and promote healing of creative fracture. Tonify liver and kidney, strengthen tendons and bones. They belong to yang tonics classified under deficiency tonics.
The guava leaf has antioxidant effect and has effect in resisting Staphylococcus aureus; has effects of eliminating dampness, invigorating spleen, clearing away heat and toxic materials, astringing, stopping bleeding, relieving inflammation, and stopping bleeding. Can be used for treating dysentery with abdominal pain, food stagnation, abdominal distention, gingival swelling and pain, rheumatalgia, eczema, ecthyma, furuncle, toxic swelling, traumatic injury, traumatic hemorrhage, and snake and insect bite.
Radix Arnebiae is dry root of arnebia euchroma (Royle) Johnst or arnebia euchroma (Royle) Johnst of Boraginaceae, and contains naphthoquinone pigment and polysaccharide compound as main ingredients, and can be released by fermentation catalysis. The lithospermum has good antibacterial, anti-tumor, antiviral, anti-inflammatory, anti-allergic, liver-protecting and enzyme-reducing effects, and is a plant with high medicinal value. The lithospermum extract has obvious bacteriostatic action on staphylococcus aureus, staphylococcus albus, pseudomonas aeruginosa, escherichia coli, typhoid bacillus, streptococcus A and streptococcus B. The naphthoquinone in the lithospermum has strong antifungal effect and has obvious therapeutic effect on candidiasis.
The borneol has the effects of dredging orifices, dispelling stagnated fire, removing nebula, improving eyesight, reducing swelling and relieving pain. It is indicated for wind stroke, vomiting, coma due to febrile disease, epilepsy due to convulsion, phlegm-phlegm, qi block, deafness, sore throat, aphtha, otitis media, carbuncle, swelling, hemorrhoid, nebula, and enterobiasis. It can also be used as cool feeling agent.
The angelica has the effects of enriching blood, activating blood, regulating menstruation, relieving pain, moistening intestines and relaxing bowels. Can be used for treating blood deficiency, sallow complexion, menoxenia, amenorrhea, dysmenorrhea, blood deficiency, blood stagnation, blood cold, traumatic injury, rheumatalgia, superficial infection, pyocutaneous disease, intestinal dryness, and constipation; the western medicine diagnosis refers to anemia due to blood deficiency of heart and liver, endometritis, functional uterine bleeding, threatened abortion, habitual abortion, postpartum hemorrhage due to blood deficiency and blood stagnation, deep muscle abscess due to blood deficiency, habitual constipation, and senile constipation or postpartum constipation due to blood deficiency and fluid depletion.
Lotus leaf can clear summer-heat, resolve dampness, raise hair, clear yang, cool blood and stop bleeding. The lotus leaf charcoal has the effects of astringing, removing blood stasis and stopping bleeding. Can be used for treating summer heat, polydipsia, summer damp diarrhea, spleen deficiency diarrhea, hematemesis, epistaxis, hematochezia, metrorrhagia, and metrostaxis. The charred folium Nelumbinis can be used for treating hemorrhage and puerperal blood faintness.
Preferably, the bacteriostatic gel further comprises at least one of the following additional technical characteristics:
the pH regulator is one or more of lactic acid, sodium lactate, acetic acid, tartaric acid, disodium hydrogen phosphate, dipotassium hydrogen phosphate, sorbic acid, citric acid and malic acid; the acidity regulator used in the invention has high safety performance and low irritation, and can regulate the pH value of the product to be in a pH range beneficial to the growth of beneficial bacteria in vagina.
The nutritional humectant comprises one or more of plant collagen, yeast-beta-glucan, alpha-glucan oligosaccharide, inulin, levan and fructo-oligosaccharide, and also comprises one or more of propylene glycol, glycerol, butanediol, pentanediol, hydrogenated castor oil, betaine, polyethylene glycol-200 and polyethylene glycol-400.
The plant collagen has extremely strong hydrophilicity, is a very good natural moisturizing component, is a non-general coverage type moisturizing agent of the plant collagen, can promote skin keratinocytes to generate natural moisturizing factors, exerts a moisturizing effect, permeates and infiltrates cuticle, enables the skin to have transparency and a moist feeling, forms a skin self-protection mechanism, enhances the skin elasticity, smoothes skin fine lines, improves the skin touch feeling, and isolates external toxic and harmful substances.
The yeast-beta-glucan can be combined with a receptor CR3 with yeast beta-glucan on immune cells Langerhans cells in an epidermal layer to activate macrophages, generate various cytokines, epidermal growth factors, tumor necrosis factors, angiogenesis factors and the like, and enhance the immunocompetence of the skin; the yeast beta-glucan has the functions of diminishing inflammation, resisting allergy, eliminating free radicals, preserving moisture, accelerating stratum corneum renewal, helping the skin to resist various exogenous mechanical and chemical stimuli, promoting wound healing, and increasing epidermal cell growth factors can improve the generation of collagen and elastin, so that the appearance of the skin is improved, and fine wrinkles disappear.
The alpha-glucan oligosaccharide, inulin, levan and fructo-oligosaccharide can promote the growth of epidermal probiotics by selecting a nutrition supply mechanism, inhibit the growth of harmful flora by a competition mechanism, establish and maintain a stable and healthy skin microecological system, maintain the normal colony balance of vagina, recover the normal pH value of the vagina, reduce inflammation and anaphylactic reaction and reduce the probability of later-period relapse.
The nutritional humectant disclosed by the invention is a natural nutritional humectant, is high in safety and low in irritation, and can be used for improving the water content of skin, forming a skin protection barrier and increasing the smoothness of a product; can also promote the reproduction of probiotic flora, inhibit the growth of harmful bacteria, ensure the maintenance of optimal acidic environment and maintain flora.
The thickening agent is one or more of methyl cellulose, carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, polyquaternium 1, polyquaternium 6, guar gum, xanthan gum, pectin, carrageenan, chitosan and chitosan;
chitosan is the only cationic biological polysaccharide in nature, has good biocompatibility, adhesiveness, degradability, bacteriostasis and plasticity, can promote wound healing and tissue regeneration, can be combined with a thickening agent to synergistically play a thickening role, and can be combined with a balanced flora composite extract to synergistically play a role in inhibiting harmful bacteria to promote the growth of probiotics, so that the vaginal flora environment is regulated to be in a balanced state.
The chelating agent is EDTA or EDTA disodium, and has a good chelating effect.
Preferably, the pH of the antibacterial gel is 4-6, and the antibacterial gel conforms to the acidity of the vaginal environment.
In another aspect of the object of the invention, a preparation method of the bacteriostatic gel is provided, which comprises the following steps:
s1, weighing oat, radix Sophorae Flavescentis, radix Paeoniae Rubra, Eucalyptus globulus Labill leaf, fructus Cnidii, herba Centellae, radix Dipsaci, folium Eucommiae, folium Psidii Guajavae, radix Arnebiae, Borneolum Syntheticum, radix Angelicae sinensis, and folium Nelumbinis according to formula ratio, mixing, pulverizing, and sterilizing;
s2, adding dication acidic ionic liquid and a microbial agent fermentation catalyst, adding deionized water, stirring uniformly, and fermenting;
s3, drying the fermented traditional Chinese medicine mixture, adding ethanol for extraction to obtain a traditional Chinese medicine liquid, and removing the ethanol in the traditional Chinese medicine liquid to obtain a fermented traditional Chinese medicine;
s4, mixing the obtained fermented traditional Chinese medicine with probiotics, a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water to prepare the antibacterial gel.
The traditional Chinese medicine fermentation is a novel traditional Chinese medicine processing technology, changes the traditional technologies of decocting, boiling, refining, boiling and the like, and can effectively enhance the biological activity of natural products, thereby improving the medicinal health-care function of the natural products. The invention adopts a method of catalyzing solid state fermentation by using dicationic acidic ionic liquid and microbial agent together, and the natural product is accompanied with the generation and conversion of various bioactive compounds in the fermentation process, thereby improving the bioavailability and bioactivity of the natural product.
Preferably, the fermentation in step S2 is anaerobic fermentation, the time of the anaerobic fermentation is 3-10 days, and the temperature of the anaerobic fermentation is 20-40 ℃.
Preferably, in step S2, the amount of the dicationic acidic ionic liquid added is 0.05-2.5% of the total mass of the traditional Chinese medicine, and the amount of the microbial agent added is 0.05-2.5% of the total mass of the traditional Chinese medicine.
Preferably, the dicationic acidic ionic liquid is added prior to or simultaneously with the microbial agent in step S2.
Preferably, step S4 specifically includes:
s41, adding the nutrient humectant of the formula amount into deionized water to prepare an aqueous solution;
s42, adding the thickening agent and the chelating agent in the formula amount into the aqueous solution, and fully swelling to prepare a matrix solution;
s43, weighing the fermented traditional Chinese medicine according to the formula amount, adding deionized water to dissolve the fermented traditional Chinese medicine, and adding the mixture into the matrix solution under the stirring condition to prepare an active solution;
s44, adding a pH regulator to regulate the pH of the active solution to 4-6, and thus obtaining the antibacterial gel.
The invention can obtain the following beneficial effects:
1. the biological activity and bioavailability of natural products in the fermented traditional Chinese medicine are greatly enhanced, so that the medicinal health-care function of the fermented traditional Chinese medicine is improved, and the prepared antibacterial gel has very excellent curative effect on female vaginitis. The fermented traditional Chinese medicine contains various natural products, is scientific and reasonable in matching, can maintain acid-base balance in the vagina, has an inhibiting effect on haemophilus vaginalis, promotes proliferation of the lactobacilli vaginalis, enhances vaginal immunity and metabolism, enables the vagina to have self-cleaning capacity and defense capacity, can clean the inside of the vagina, can improve vaginal health and vaginal verification, is high in safety performance, and has no stimulation to skin.
2. This scheme is through scientific and reasonable's collocation, and multiple bacteriostatic composition combines, at the growth that restraines the harmful bacterium, plays the synergism in the aspect of promoting the growth of living fungus, and thickener and chelator combine to play the synergism in the aspect of increasing product viscosity, make product convenient to use, and the effective component concentration suitably increases, and the effect of nutrition humectant forms the protection barrier of skin in addition simultaneously, increases its lubricity.
3. The antibacterial gel can effectively inhibit the growth of harmful vaginal pathogenic bacteria, dispel the symptoms such as burning sensation, itching peculiar smell and the like at private parts, promote the growth and reproduction of vaginal probiotic groups, adjust the clean and balanced environment in the vagina and achieve the aim of daily maintenance of the vagina; meanwhile, the water is quickly supplemented and nourished to a private ecosystem, the repairing is continuously and mildly carried out, no stimulation is caused to mucosa, the microcirculation of a female reproductive system is quickly repaired, and the incidence rate of gynecological inflammation is reduced.
4. The antibacterial gel prepared by the invention does not contain any antibiotic, does not generate any drug resistance, does not contain a bactericide, reduces the probability of adverse reaction, and is a multipurpose gel with antibacterial balance, which can be used for gynecological adjuvant therapy and female vaginal daily maintenance.
Drawings
FIG. 1 is a flow chart of a method for preparing a bacteriostatic gel according to a preferred embodiment of the invention;
fig. 2 is a flow chart of a preparation method of the dicationic acidic ionic liquid according to the preferred embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The chemical reagents and laboratory equipment used in the preferred embodiment of the invention are commercially available.
The preparation process of dicationic acidic ionic liquid is shown in figure 2, and ionic liquid [ Ps2TMEDA][BF4]2Is an example.
Adding tetramethylethylenediamine and ethanol (1 mol: 1L) into a reactor respectively, and preheating at 70 ℃; 1, 3-propane sultone (2 times of the amount of the substance of tetramethylethylenediamine) was added dropwise under stirring, and after completion of the dropwise addition, stirring was continued for 8 hours to obtain a large amount of an intermediate (white solid). After the reaction is finished, the intermediate product is repeatedly washed by ether for 3-5 times to remove unreacted raw materials, and then the intermediate product is dried in a vacuum drying oven at 80 ℃ for 12 hours.
The intermediate product was added to the reactor, preheated at 70 ℃, and then a tetrafluoroboric acid solution (2 times the amount of tetramethylethylenediamine) was added dropwise to the reactor, with continued stirring for 10 h. After the reaction is finished, removing water in the reaction mixture by rotary evaporation, and finally washing the obtained product for 3-5 times by using acetone to remove the intermediate product which is possibly not reacted, thereby obtaining the ionic liquid [ Ps ]2TMEDA][BF4]2Vacuum drying at 80 deg.C for 10h gave a yield of about 92%.
With reference to the above method, the intermediateThe product reacts with sulfuric acid solution, phosphoric acid solution, hydrochloric acid solution, nitric acid solution, trifluoromethanesulfonic acid solution or hexafluorophosphoric acid solution respectively at 75 ℃ for 10h to prepare ionic liquid [ Ps2TMEDA][HSO4]2、[Ps2TMEDA][H2PO4]2、[Ps2TMEDA]Cl2、[Ps2TMEDA][NO3]2、[Ps2TMEDA][CF3SO3]2、[Ps2TMEDA][PCl6]2The yields of the six ionic liquids were 92%, 90%, 92%, respectively.
1, 3-propane sultone is replaced by 1, 4-butane sultone, and the ionic liquid [ Bs ] can be prepared according to the same steps2TMEDA][BF4]2、[Bs2TMEDA][HSO4]2、[Bs2TMEDA][H2PO4]2、[Bs2TMEDA]Cl2、[Bs2TMEDA][NO3]2、[Bs2TMEDA][CF3SO3]2、[Bs2TMEDA][PCl6]2The yields of the seven ionic liquids were 92%, 91%, 90%, 92%, 90%, 92%, respectively.
The dicationic acidic ionic liquid prepared by the above method was used in the following examples as shown in fig. 1.
Example 1:
a bacteriostatic gel comprising: through [ Ps ]2TMEDA][BF4]2And traditional Chinese medicines fermented and catalyzed by microbial agent fermentation, probiotics, a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water.
The preparation method comprises the following steps:
s1, weighing the following traditional Chinese medicines in parts by weight according to the formula proportion: 40 parts of oat, 30 parts of radix sophorae flavescentis, 30 parts of red peony root, 25 parts of eucalyptus globulus, 25 parts of fructus cnidii, 25 parts of centella, 25 parts of teasel root, 20 parts of eucommia ulmoides leaf, 20 parts of guava leaf, 20 parts of lithospermum, 10 parts of borneol, 10 parts of angelica and 10 parts of lotus leaf, mixing, crushing and sterilizing;
s2, adding [ Ps ] with the total mass of 1.5 percent of the traditional Chinese medicine2TMEDA][BF4]2And 0.5% of acetobacter xylinum, 0.5% of lactobacillus plantarum and 0.5% of lactobacillus acidophilus are taken as fermentation catalysts, ionized water with the total mass of 10% of the traditional Chinese medicines is added, the mixture is stirred uniformly, and anaerobic fermentation is carried out; wherein the anaerobic fermentation time is 6 days, and the temperature is 30 ℃;
s3, drying the fermented traditional Chinese medicine mixture, adding ethanol for extraction to obtain a traditional Chinese medicine liquid, and performing rotary evaporation to remove the ethanol in the traditional Chinese medicine liquid to obtain a fermented traditional Chinese medicine;
s4, adding 1 part of plant collagen, 1 part of yeast-beta-glucan, 1 part of inulin, 1 part of propylene glycol, 1 part of hydrogenated castor oil and 2001 parts of polyethylene glycol into 60 parts of deionized water to prepare an aqueous solution;
adding 1 part of carboxymethyl cellulose, 0.5 part of xanthan gum, 0.5 part of carrageenan, 0.5 part of chitosan and 2.5 parts of EDTA into the aqueous solution, and fully swelling to prepare a matrix solution;
taking 6 parts of fermented traditional Chinese medicine, adding 20 parts of deionized water to dissolve the fermented traditional Chinese medicine, adding the mixture into the matrix solution under the condition of stirring to prepare an active solution;
adding 2 parts of lactic acid and 1 part of sodium lactate to adjust the pH value of the active solution to 4-6, thus obtaining the antibacterial gel.
Example 2:
a bacteriostatic gel comprising: through [ Ps ]2TMEDA][BF4]2And traditional Chinese medicines fermented and catalyzed by microbial agent fermentation, probiotics, a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water.
The preparation method comprises the following steps:
s1, weighing the following traditional Chinese medicines in parts by weight according to the formula proportion: 40 parts of oat, 30 parts of radix sophorae flavescentis, 30 parts of red peony root, 25 parts of eucalyptus globulus, 25 parts of fructus cnidii, 25 parts of centella, 25 parts of teasel root, 20 parts of eucommia ulmoides leaf, 20 parts of guava leaf, 20 parts of lithospermum, 10 parts of borneol, 10 parts of angelica and 10 parts of lotus leaf, mixing, crushing and sterilizing;
s2, adding [ Ps ] with the total mass of 1.5 percent of the traditional Chinese medicine2TMEDA][BF4]2As fermentation catalystsAdding ionized water with the total mass of 10% of the traditional Chinese medicines, stirring uniformly, and performing anaerobic fermentation; wherein the anaerobic fermentation time is 2 days, and the temperature is 30 ℃; then 0.5% of acetobacter xylinum, 0.5% of lactobacillus plantarum and 0.5% of lactobacillus acidophilus are added as fermentation catalysts, and anaerobic fermentation is continued for 4 days.
S3, drying the fermented traditional Chinese medicine mixture, adding ethanol for extraction to obtain a traditional Chinese medicine liquid, and performing rotary evaporation to remove the ethanol in the traditional Chinese medicine liquid to obtain a fermented traditional Chinese medicine;
s4, adding 1 part of plant collagen, 1 part of yeast-beta-glucan, 1 part of inulin, 1 part of propylene glycol, 1 part of hydrogenated castor oil and 2001 parts of polyethylene glycol into 60 parts of deionized water to prepare an aqueous solution;
adding 1 part of carboxymethyl cellulose, 0.5 part of xanthan gum, 0.5 part of carrageenan, 0.5 part of chitosan and 2.5 parts of EDTA into the aqueous solution, and fully swelling to prepare a matrix solution;
taking 6 parts of fermented traditional Chinese medicine, adding 20 parts of deionized water to dissolve the fermented traditional Chinese medicine, adding the mixture into the matrix solution under the condition of stirring to prepare an active solution;
adding 2 parts of lactic acid and 1 part of sodium lactate to adjust the pH value of the active solution to 4-6, thus obtaining the antibacterial gel.
Example 3:
a bacteriostatic gel comprising: through [ Ps ]2TMEDA][HSO4]2And traditional Chinese medicines fermented and catalyzed by microbial agent fermentation, probiotics, a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water.
The preparation method comprises the following steps:
s1, weighing the following traditional Chinese medicines in parts by weight according to the formula proportion: 20 parts of oat, 50 parts of radix sophorae flavescentis, 30 parts of red peony root, 40 parts of eucalyptus globulus, 30 parts of fructus cnidii, 10 parts of centella, 10 parts of teasel root, 10 parts of folium cortex eucommiae, 10 parts of guava leaf, 30 parts of lithospermum, 20 parts of borneol, 20 parts of angelica and 20 parts of lotus leaf, mixing, crushing and sterilizing;
s2, adding [ Ps ] with the total mass of the traditional Chinese medicine of 1%2TMEDA][HSO4]2Adding ionized water with a concentration of 10% of the total mass of the Chinese medicinal materials as fermentation catalyst, and stirringUniformly performing anaerobic fermentation; wherein the anaerobic fermentation time is 2 days, and the temperature is 30 ℃; then adding 1% of lactobacillus plantarum, 0.5% of saccharomycete and 0.5% of lactobacillus acidophilus as fermentation catalysts, and continuing anaerobic fermentation for 6 days.
S3, drying the fermented traditional Chinese medicine mixture, adding ethanol for extraction to obtain a traditional Chinese medicine liquid, and performing rotary evaporation to remove the ethanol in the traditional Chinese medicine liquid to obtain a fermented traditional Chinese medicine;
s4, adding 2 parts of plant collagen, 2 parts of alpha-glucan oligosaccharide, 2002 parts of polyethylene glycol and 2 parts of pentanediol into 60 parts of deionized water to prepare an aqueous solution;
adding 1 part of hydroxyethyl cellulose, 1 part of hydroxypropyl methyl cellulose, 0.5 part of carrageenan, 0.5 part of chitosan and 2 parts of EDTA into the aqueous solution, and fully swelling to prepare a matrix solution;
taking 3 parts of fermented traditional Chinese medicine, adding 20 parts of deionized water to dissolve the fermented traditional Chinese medicine, adding the mixture into the matrix solution under the condition of stirring to prepare an active solution;
adding 1.5 parts of lactic acid and 1.5 parts of citric acid to adjust the pH of the active solution to 4-6, thus obtaining the antibacterial gel.
Example 4:
a bacteriostatic gel comprising: through [ Bs ]2TMEDA][H2PO4]2And traditional Chinese medicines fermented and catalyzed by microbial agent fermentation, probiotics, a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water.
The preparation method comprises the following steps:
s1, weighing the following traditional Chinese medicines in parts by weight according to the formula proportion: 30 parts of oat, 40 parts of radix sophorae flavescentis, 40 parts of red peony root, 20 parts of eucalyptus globulus, 20 parts of fructus cnidii, 20 parts of centella, 20 parts of teasel root, 30 parts of folium cortex eucommiae, 30 parts of guava leaf, 10 parts of lithospermum, 15 parts of borneol, 15 parts of angelica and 15 parts of lotus leaf, mixing, crushing and sterilizing;
s2, adding Bs accounting for 2 percent of the total mass of the traditional Chinese medicines2TMEDA][H2PO4]2Adding ionized water with the total mass of 10% of the traditional Chinese medicines as a fermentation catalyst, stirring uniformly, and performing anaerobic fermentation; wherein the anaerobic partThe time of oxygen fermentation is 2 days, and the temperature is 25 ℃; then adding yeast 1.5%, acetobacter xylinum 0.5% and lactobacillus acidophilus 0.5% as fermentation catalysts, and continuing anaerobic fermentation for 4 days.
S3, drying the fermented traditional Chinese medicine mixture, adding ethanol for extraction to obtain a traditional Chinese medicine liquid, and performing rotary evaporation to remove the ethanol in the traditional Chinese medicine liquid to obtain a fermented traditional Chinese medicine;
s4, adding 1.5 parts of yeast-beta-glucan, 1.5 parts of alpha-glucan oligosaccharide, 1.5 parts of fructan, 1.5 parts of butanediol and 1.5 parts of inulin into 60 parts of deionized water to prepare an aqueous solution;
adding 0.5 part of hydroxypropyl methyl cellulose, 10.5 parts of polyquaternium, 0.5 part of pectin, 0.5 part of chitosan and 3 parts of EDTA disodium into the aqueous solution, and fully swelling to prepare a matrix solution;
taking 5 parts of fermented traditional Chinese medicine, adding 20 parts of deionized water to dissolve the fermented traditional Chinese medicine, and adding the mixture into the matrix solution under the stirring condition to prepare an active solution;
adding 1 part of lactic acid, 0.5 part of acetic acid and 0.5 part of dipotassium phosphate to adjust the pH value of the active solution to 4-6, and thus obtaining the antibacterial gel.
Example 5:
a bacteriostatic gel comprising: through [ Bs ]2TMEDA]Cl2And traditional Chinese medicines fermented and catalyzed by microbial agent fermentation, probiotics, a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water.
The preparation method comprises the following steps:
s1, weighing the following traditional Chinese medicines in parts by weight according to the formula proportion: 50 parts of oat, 25 parts of radix sophorae flavescentis, 25 parts of red peony root, 25 parts of eucalyptus globulus, 25 parts of fructus cnidii, 25 parts of centella, 25 parts of teasel root, 10 parts of eucommia ulmoides leaf, 10 parts of guava leaf, 10 parts of lithospermum, 30 parts of borneol, 30 parts of angelica and 30 parts of lotus leaf, mixing, crushing and sterilizing;
s2, adding 0.5 percent of [ Bs ] in the total mass of the traditional Chinese medicines2TMEDA]Cl2Adding ionized water with the total mass of 10% of the traditional Chinese medicines as a fermentation catalyst, stirring uniformly, and performing anaerobic fermentation; wherein the anaerobic fermentation time is 3 days, and the temperature is 35 ℃; then adding 1 percentAcetobacter xylinum, 0.5% of lactobacillus plantarum, 0.5% of lactobacillus acidophilus and 0.5% of lactobacillus bifidus are used as fermentation catalysts, and anaerobic fermentation is continued for 6 days.
S3, drying the fermented traditional Chinese medicine mixture, adding ethanol for extraction to obtain a traditional Chinese medicine liquid, and performing rotary evaporation to remove the ethanol in the traditional Chinese medicine liquid to obtain a fermented traditional Chinese medicine;
s4, adding 3 parts of plant collagen, 3 parts of yeast-beta-glucan and 3 parts of betaine into 60 parts of deionized water to prepare an aqueous solution;
adding 0.75 part of methyl cellulose, 0.75 part of xanthan gum, 0.75 part of chitosan and 4 parts of EDTA disodium into the aqueous solution, and fully swelling to prepare a matrix solution;
taking 8 parts of fermented traditional Chinese medicine, adding 20 parts of deionized water to dissolve the fermented traditional Chinese medicine, adding the mixture into the matrix solution under the condition of stirring to prepare an active solution;
adding 2 parts of lactic acid, 1 part of dipotassium hydrogen phosphate and 0.5 part of malic acid to adjust the pH value of the active solution to 4-6, and thus obtaining the antibacterial gel.
Example 6:
a bacteriostatic gel comprising: through [ Bs ]2TMEDA][NO3]2And traditional Chinese medicines fermented and catalyzed by microbial agent fermentation, probiotics, a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water.
The preparation method comprises the following steps:
s1, weighing the following traditional Chinese medicines in parts by weight according to the formula proportion: 35 parts of oat, 35 parts of radix sophorae flavescentis, 35 parts of red peony root, 15 parts of eucalyptus globulus, 15 parts of fructus cnidii, 15 parts of centella, 35 parts of teasel root, 25 parts of folium cortex eucommiae, 15 parts of guava leaf, 25 parts of lithospermum, 5 parts of borneol, 5 parts of angelica and 5 parts of lotus leaf, mixing, crushing and sterilizing;
s2, adding [ Bs ] accounting for 2.5 percent of the total mass of the traditional Chinese medicines2TMEDA][NO3]2Adding ionized water with the total mass of 10% of the traditional Chinese medicines as a fermentation catalyst, stirring uniformly, and performing anaerobic fermentation; wherein the anaerobic fermentation time is 2 days, and the temperature is 30 ℃; adding 0.5% Lactobacillus plantarum and 0.5% BifidobacteriumAnaerobic fermentation was continued for 5 days as a fermentation catalyst.
S3, drying the fermented traditional Chinese medicine mixture, adding ethanol for extraction to obtain a traditional Chinese medicine liquid, and performing rotary evaporation to remove the ethanol in the traditional Chinese medicine liquid to obtain a fermented traditional Chinese medicine;
s4, adding 2 parts of fructo-oligosaccharide, 2 parts of alpha-glucan oligosaccharide, 1.5 parts of glycerol and 4001.5 parts of polyethylene glycol into 60 parts of deionized water to prepare an aqueous solution;
adding 1 part of pectin, 1 part of xanthan gum, 0.5 part of guar gum, 0.5 part of chitosan and 5 parts of EDTA into an aqueous solution, and fully swelling to prepare a matrix solution;
taking 2 parts of fermented traditional Chinese medicine, adding 20 parts of deionized water to dissolve the fermented traditional Chinese medicine, adding the mixture into the matrix solution under the condition of stirring to prepare an active solution;
adding 1 part of lactic acid, 1 part of citric acid and 0.5 part of malic acid to adjust the pH of the active solution to 4-6, thus obtaining the antibacterial gel.
Example 7:
a bacteriostatic gel comprising: through [ Bs ]2TMEDA][CF3SO3]2And traditional Chinese medicines fermented and catalyzed by microbial agent fermentation, probiotics, a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water.
The preparation method comprises the following steps:
s1, weighing the following traditional Chinese medicines in parts by weight according to the formula proportion: 30 parts of oat, 30 parts of radix sophorae flavescentis, 30 parts of red peony root, 30 parts of eucalyptus globulus, 30 parts of fructus cnidii, 30 parts of centella, 30 parts of teasel root, 15 parts of folium cortex eucommiae, 15 parts of guava leaf, 30 parts of lithospermum, 10 parts of borneol, 10 parts of angelica and 12 parts of lotus leaf, mixing, crushing and sterilizing;
s2, adding 0.2 percent of [ Bs ] in the total mass of the traditional Chinese medicines2TMEDA][CF3SO3]2Adding ionized water with the total mass of 10% of the traditional Chinese medicines as a fermentation catalyst, stirring uniformly, and performing anaerobic fermentation; wherein the anaerobic fermentation time is 3 days, and the temperature is 30 ℃; then adding 1.5% of lactobacillus plantarum, 0.5% of yeast and 0.5% of lactobacillus acidophilus as fermentation catalysts, and continuing anaerobic fermentation for 6 days.
S3, drying the fermented traditional Chinese medicine mixture, adding ethanol for extraction to obtain a traditional Chinese medicine liquid, and performing rotary evaporation to remove the ethanol in the traditional Chinese medicine liquid to obtain a fermented traditional Chinese medicine;
s4, adding 0.5 part of plant collagen, 0.5 part of betaine, 0.5 part of alpha-glucan oligosaccharide, 0.5 part of inulin and 0.5 part of propylene glycol into 60 parts of deionized water to prepare an aqueous solution;
adding 1 part of methyl cellulose, 0.5 part of guar gum, 0.5 part of carrageenan, 60.5 parts of polyquaternium and 2.5 parts of EDTA into the aqueous solution, and fully swelling to prepare a matrix solution;
taking 9 parts of fermented traditional Chinese medicine, adding 20 parts of deionized water to dissolve the fermented traditional Chinese medicine, adding the mixture into the matrix solution under the condition of stirring to prepare an active solution;
adding 1 part of lactic acid, 1 part of tartaric acid and 1 part of disodium hydrogen phosphate to adjust the pH of the active solution to 4-6, thus obtaining the antibacterial gel.
Example 8:
a bacteriostatic gel comprising: through [ Ps ]2TMEDA][PCl6]2And traditional Chinese medicines fermented and catalyzed by microbial agent fermentation, probiotics, a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water.
The preparation method comprises the following steps:
s1, weighing the following traditional Chinese medicines in parts by weight according to the formula proportion: 45 parts of oat, 45 parts of radix sophorae flavescentis, 45 parts of red peony root, 25 parts of eucalyptus globulus, 25 parts of fructus cnidii, 25 parts of centella, 20 parts of teasel root, 20 parts of folium cortex eucommiae, 20 parts of guava leaf, 20 parts of lithospermum, 12 parts of borneol, 10 parts of angelica and 10 parts of lotus leaf, mixing, crushing and sterilizing;
s2, adding [ Ps ] accounting for 1.8 percent of the total mass of the traditional Chinese medicines2TMEDA][PCl6]2Adding ionized water with the total mass of 10% of the traditional Chinese medicines as a fermentation catalyst, stirring uniformly, and performing anaerobic fermentation; wherein the anaerobic fermentation time is 2 days, and the temperature is 25 ℃; then adding yeast 0.5%, lactobacillus bifidus 0.5%, and lactobacillus acidophilus 0.5% as fermentation catalyst, and continuing anaerobic fermentation for 5 days.
S3, drying the fermented traditional Chinese medicine mixture, adding ethanol for extraction to obtain a traditional Chinese medicine liquid, and performing rotary evaporation to remove the ethanol in the traditional Chinese medicine liquid to obtain a fermented traditional Chinese medicine;
s4, adding 1.5 parts of plant collagen, 1.5 parts of yeast-beta-glucan, 1 part of pentanediol, 1 part of hydrogenated castor oil and 2001 parts of polyethylene glycol into 60 parts of deionized water to prepare an aqueous solution;
adding 1 part of chitosan, 60.5 parts of polyquaternium, 0.5 part of carrageenan, 0.5 part of chitosan and 2.5 parts of EDTA into the aqueous solution, and fully swelling to prepare a matrix solution;
taking 3.5 parts of fermented traditional Chinese medicine, adding 20 parts of deionized water to dissolve the fermented traditional Chinese medicine, adding the mixture into the matrix solution under the condition of stirring to prepare an active solution;
adding 1 part of lactic acid and 0.5 part of tartaric acid to adjust the pH of the active solution to 4-6, thus obtaining the antibacterial gel.
Comparative example 1:
the fermented Chinese medicinal materials were replaced with the decocted Chinese medicinal extract, and the rest was the same as in example 2.
Comparative example 2:
without adding dicationic acidic ionic liquid [ Ps2TMEDA][BF4]2The procedure of example 2 was repeated except that the fermentation was carried out for 6 days using a microbial agent.
Comparative example 3:
the plant collagen, yeast-beta-glucan and inulin were removed, the ratio of other components in the nutritional moisturizer was increased, and the rest was the same as in example 2.
Comparative example 4:
the procedure of example 2 was repeated except that no chelating agent was added.
The effect evaluation was performed on the bacteriostatic gels of examples 1 to 8 and comparative examples 1 to 4.
Test 1: and (3) testing the antibacterial effect: the test method refers to the evaluation method of the antibacterial and bacteriostatic effects of the light industry standard QB/T2738-.
TABLE 1
Figure BDA0002833754000000191
Figure BDA0002833754000000201
The data in Table 1 show that the formula products of the female private nursing gel have obvious antibacterial effect, the antibacterial rate to Escherichia coli, staphylococcus aureus and Candida albicans is over 93.5%, and the comparison between example 1 and example 2 shows that the dicationic acidic ionic liquid is added before the microbial agent to obtain higher antibacterial effect; the ionic liquid can destroy the crystal structure of Chinese medicine fiber to raise the dissolubility of Chinese medicine, promote the dissolution of active matter in Chinese medicine, speed the fermentation of microbial preparation and raise the available active components and bioavailability of Chinese medicine. Compared with the comparative example 1, the fermentation of the traditional Chinese medicine can greatly improve the biological activity and the utilization rate of the traditional Chinese medicine, and the bacteriostasis rate of the bacteriostasis gel is obvious; compared with the comparative example 2, the dicationic acid ionic liquid can generate a synergistic effect with the microbial agent, and the available active ingredients of the traditional Chinese medicine and the bioavailability thereof are improved, so that the bacteriostasis rate of the bacteriostasis gel is improved; compared with the comparative example 3, the addition of functional humectants such as plant collagen, yeast-beta-glucan, inulin and the like can improve the bacteriostasis rate of the bacteriostasis gel; compared with comparative example 4, the chelating agent is added to enhance the thixotropy of the gel, promote the drug effect to be exerted and improve the bacteriostasis rate of the bacteriostasis gel.
Test 2: beneficial bacteria (Lactobacillus acidophilus GDMCC1.208) growth effect test: preparation of a Lactobacillus acidophilus suspension (1.0X 10)4～9.0×104) Taking 5mL of female privacy nursing gel diluent (1:10), adding 0.1mL of acidophilic lactobacillus suspension, after acting for 2min, putting the mixture into an MSR culture medium, culturing for 12h and 24h at 37 ℃, counting viable bacterial colonies, and calculating the survival rate of lactobacillus, wherein the results are shown in Table 2. The survival rate is calculated according to the following formula:
survival rate 1- (number of colonies before treatment-number of colonies after treatment)/number of colonies before treatment × 100%
TABLE 2
Figure BDA0002833754000000211
Figure BDA0002833754000000221
Experiments show that the vaginal beneficial bacteria growth-promoting agent has no obvious effect on vaginal beneficial bacteria and high survival rate of vaginal beneficial bacteria, shows that the vaginal pathogenic bacteria are killed, the vaginal beneficial bacteria growth is slightly influenced, the beneficial bacteria growth can be promoted, the vaginal pathogenic bacteria growth-promoting agent is safe to use for a long time, and the vaginal beneficial bacteria growth-promoting agent has a good effect on treatment and prevention of vaginitis. Compared with comparative examples 1-4, the results show that traditional Chinese medicine fermentation, dicationic acidic ionic liquid, functional humectants such as plant collagen, yeast-beta-glucan, inulin and the like, and chelating agents all play a beneficial role in survival of beneficial bacteria, the reasons of the beneficial bacteria are probably similar to data analysis in table 1, harmful bacteria and beneficial bacteria are in a mutual competitive relationship in growth, and the survival rate of the beneficial bacteria is improved while the harmful bacteria are inhibited; wherein, the traditional Chinese medicine fermentation has the biggest influence on the survival rate of beneficial bacteria.
As can be seen from the data in Table 2, the best solution of the present invention is shown in example 2, and the contents of the components in the formula can satisfy the killing effect on harmful bacteria, promote the growth of probiotics, and maintain the normal colony balance of the vagina. The bacteriostatic gel is simple in process and beneficial to industrial production.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims (10)

1. A bacteriostatic gel, comprising: the traditional Chinese medicine fermentation product comprises a fermentation traditional Chinese medicine, a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water which are subjected to fermentation catalysis by a dicationic acidic ionic liquid and a microbial agent.
2. A bacteriostatic gel according to claim 1, wherein the dicationic acidic ionic liquid is sulfonic acid-functionalized tetramethylethylenediamine ionic liquid.
3. A bacteriostatic gel according to claim 2, wherein the preparation method of the dicationic acidic ionic liquid comprises the following steps:
a. adding 1, 3-propane sultone or 1, 4-propane sultone into an ethanol solution of tetramethylethylenediamine under the condition of stirring, reacting for a period of time at 60-100 ℃, and separating the obtained solid intermediate product;
b. and (4) adding an acid solution into the solid intermediate product obtained in the step S1, stirring and reacting for a period of time at the temperature of 60-100 ℃, and removing the solvent and residual reactants in the reaction mixture to obtain the dicationic acidic ionic liquid.
4. The bacteriostatic gel according to claim 1, wherein the traditional Chinese medicines comprise: 20-50 parts of oat, 20-50 parts of radix sophorae flavescentis, 20-50 parts of red peony root, 10-40 parts of eucalyptus globulus leaf, 10-40 parts of fructus cnidii, 10-40 parts of centella, 10-40 parts of teasel root, 10-30 parts of folium cortex eucommiae, 10-30 parts of guava leaf, 10-30 parts of lithospermum, 0-20 parts of borneol, 0-20 parts of angelica sinensis and 0-20 parts of lotus leaf.
5. The bacteriostatic gel according to claim 1, wherein the microbial agent comprises one or more of yeast, acetobacter xylinum, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus bifidus.
6. A bacteriostatic gel according to claim 1, characterized by further comprising at least one of the following additional technical features:
the pH regulator is one or more of lactic acid, sodium lactate, acetic acid, tartaric acid, disodium hydrogen phosphate, dipotassium hydrogen phosphate, citric acid and malic acid;
the nutritional humectant comprises one or more of plant collagen, yeast-beta-glucan, alpha-glucan oligosaccharide, inulin, levan and fructo-oligosaccharide, and also comprises one or more of propylene glycol, glycerol, butanediol, pentanediol, hydrogenated castor oil, betaine, sodium lactate, polyethylene glycol-200 and polyethylene glycol-400;
the thickening agent is one or more of methyl cellulose, carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, polyquaternium 1, polyquaternium 6, guar gum, xanthan gum, pectin, carrageenan, chitosan and chitosan;
the chelating agent is EDTA or EDTA disodium.
7. A bacteriostatic gel according to claim 1, wherein the pH is 4-6.
8. The preparation method of the antibacterial gel is characterized by comprising the following steps:
s1, weighing oat, radix Sophorae Flavescentis, radix Paeoniae Rubra, Eucalyptus globulus Labill leaf, fructus Cnidii, herba Centellae, radix Dipsaci, folium Eucommiae, folium Psidii Guajavae, radix Arnebiae, Borneolum Syntheticum, radix Angelicae sinensis, and folium Nelumbinis according to formula ratio, mixing, pulverizing, and sterilizing;
s2, adding dication acidic ionic liquid and a microbial agent fermentation catalyst, adding deionized water, stirring uniformly, and fermenting;
s3, drying the fermented traditional Chinese medicine mixture, adding ethanol for extraction to obtain a traditional Chinese medicine liquid, and removing the ethanol in the traditional Chinese medicine liquid to obtain a fermented traditional Chinese medicine;
s4, mixing the obtained fermented traditional Chinese medicine with a pH regulator, a nutrition humectant, a thickening agent, a chelating agent and deionized water to prepare the antibacterial gel.
9. A method for preparing a bacteriostatic gel according to claim 8, characterized by further comprising at least one of the following additional technical features:
in the step S2, the fermentation is anaerobic fermentation, the time of the anaerobic fermentation is 3-10 days, and the temperature of the anaerobic fermentation is 20-40 ℃;
in the step S2, the addition amount of the dicationic acidic ionic liquid is 0.05-2.5% of the total mass of the traditional Chinese medicine, and the addition amount of the microbial agent is 0.05-2.5% of the total mass of the traditional Chinese medicine;
in step S2, the dicationic acidic ionic liquid is added prior to or simultaneously with the microbial agent.
10. The method for preparing a bacteriostatic gel according to claim 8, wherein the step S4 specifically comprises:
s41, adding the nutrient humectant of the formula amount into deionized water to prepare an aqueous solution;
s42, adding the thickening agent and the chelating agent in the formula amount into the aqueous solution, and fully swelling to prepare a matrix solution;
s43, weighing the fermented traditional Chinese medicine according to the formula amount, adding deionized water to dissolve the fermented traditional Chinese medicine, and adding the mixture into the matrix solution under the stirring condition to prepare an active solution;
s44, adding a pH regulator to regulate the pH of the active solution to 4-6, and thus obtaining the antibacterial gel.
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