CN112370489A - Antibacterial gel for restoring ecological balance of women and preparation method thereof - Google Patents
Antibacterial gel for restoring ecological balance of women and preparation method thereof Download PDFInfo
- Publication number
- CN112370489A CN112370489A CN202011314033.5A CN202011314033A CN112370489A CN 112370489 A CN112370489 A CN 112370489A CN 202011314033 A CN202011314033 A CN 202011314033A CN 112370489 A CN112370489 A CN 112370489A
- Authority
- CN
- China
- Prior art keywords
- parts
- ecological balance
- plant
- gel
- raw materials
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 60
- 238000002360 preparation method Methods 0.000 title abstract description 11
- 229960002233 benzalkonium bromide Drugs 0.000 claims abstract description 47
- KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 claims abstract description 47
- 230000003385 bacteriostatic effect Effects 0.000 claims abstract description 42
- 102000008186 Collagen Human genes 0.000 claims abstract description 38
- 108010035532 Collagen Proteins 0.000 claims abstract description 38
- 229920001436 collagen Polymers 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 35
- 239000002994 raw material Substances 0.000 claims abstract description 34
- 239000008213 purified water Substances 0.000 claims abstract description 26
- SNCIAAAFBLUWEE-NMTVEPIMSA-N CSCC[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(C)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](C)C(O)=O Chemical compound CSCC[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(C)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](C)C(O)=O SNCIAAAFBLUWEE-NMTVEPIMSA-N 0.000 claims abstract description 21
- 150000004676 glycans Chemical class 0.000 claims abstract description 20
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 20
- 239000005017 polysaccharide Substances 0.000 claims abstract description 20
- 239000000419 plant extract Substances 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 239000011159 matrix material Substances 0.000 claims abstract description 16
- 230000008961 swelling Effects 0.000 claims abstract description 16
- 239000004480 active ingredient Substances 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 7
- 238000007865 diluting Methods 0.000 claims abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 50
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 39
- 239000000243 solution Substances 0.000 claims description 27
- 239000000203 mixture Substances 0.000 claims description 13
- 239000000284 extract Substances 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 12
- 239000000341 volatile oil Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 10
- 229920002125 Sokalan® Polymers 0.000 claims description 9
- WDQLRUYAYXDIFW-RWKIJVEZSA-N (2r,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-4-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-[[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](CO)O[C@@H](O)[C@H](O)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@@H](CO[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)O1 WDQLRUYAYXDIFW-RWKIJVEZSA-N 0.000 claims description 8
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical group OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 8
- 240000002045 Guettarda speciosa Species 0.000 claims description 8
- 235000001287 Guettarda speciosa Nutrition 0.000 claims description 8
- 229920002305 Schizophyllan Polymers 0.000 claims description 8
- 229960001631 carbomer Drugs 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 244000178870 Lavandula angustifolia Species 0.000 claims description 6
- 235000010663 Lavandula angustifolia Nutrition 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000001102 lavandula vera Substances 0.000 claims description 6
- 235000018219 lavender Nutrition 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 244000020518 Carthamus tinctorius Species 0.000 claims description 5
- 235000003255 Carthamus tinctorius Nutrition 0.000 claims description 5
- 235000000604 Chrysanthemum parthenium Nutrition 0.000 claims description 5
- 235000000802 Leonurus cardiaca ssp. villosus Nutrition 0.000 claims description 5
- 241000750718 Pterocarpus santalinus Species 0.000 claims description 4
- 241000304195 Salvia miltiorrhiza Species 0.000 claims description 4
- 235000011135 Salvia miltiorrhiza Nutrition 0.000 claims description 4
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical group OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 4
- 239000003085 diluting agent Substances 0.000 claims description 4
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 238000004090 dissolution Methods 0.000 claims description 3
- 239000003995 emulsifying agent Substances 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 238000005325 percolation Methods 0.000 claims description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229920000053 polysorbate 80 Polymers 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 244000301850 Cupressus sempervirens Species 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 240000007890 Leonurus cardiaca Species 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 46
- 239000003814 drug Substances 0.000 abstract description 6
- 230000001225 therapeutic effect Effects 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract 1
- 239000000499 gel Substances 0.000 description 68
- 239000000047 product Substances 0.000 description 44
- 230000009286 beneficial effect Effects 0.000 description 24
- 210000003491 skin Anatomy 0.000 description 18
- 230000001737 promoting effect Effects 0.000 description 14
- 208000003251 Pruritus Diseases 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 9
- 230000004888 barrier function Effects 0.000 description 9
- 235000019441 ethanol Nutrition 0.000 description 9
- 230000002147 killing effect Effects 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 208000002193 Pain Diseases 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 230000001086 cytosolic effect Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 230000036407 pain Effects 0.000 description 6
- 239000006041 probiotic Substances 0.000 description 6
- 235000018291 probiotics Nutrition 0.000 description 6
- 230000008929 regeneration Effects 0.000 description 6
- 238000011069 regeneration method Methods 0.000 description 6
- 150000003384 small molecules Chemical class 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 210000001215 vagina Anatomy 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 241000222122 Candida albicans Species 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 5
- 241000191967 Staphylococcus aureus Species 0.000 description 5
- 229940095731 candida albicans Drugs 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 5
- 230000007803 itching Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 230000017531 blood circulation Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000002708 enhancing effect Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 241000411851 herbal medicine Species 0.000 description 4
- 230000028993 immune response Effects 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 241000195493 Cryptophyta Species 0.000 description 3
- 201000004624 Dermatitis Diseases 0.000 description 3
- 102000016942 Elastin Human genes 0.000 description 3
- 108010014258 Elastin Proteins 0.000 description 3
- 241000207925 Leonurus Species 0.000 description 3
- 208000025865 Ulcer Diseases 0.000 description 3
- 230000003213 activating effect Effects 0.000 description 3
- 230000032683 aging Effects 0.000 description 3
- 239000003899 bactericide agent Substances 0.000 description 3
- 239000013060 biological fluid Substances 0.000 description 3
- 239000003093 cationic surfactant Substances 0.000 description 3
- 230000021164 cell adhesion Effects 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000005536 corrosion prevention Methods 0.000 description 3
- 230000007123 defense Effects 0.000 description 3
- 230000000249 desinfective effect Effects 0.000 description 3
- 229920002549 elastin Polymers 0.000 description 3
- 210000002919 epithelial cell Anatomy 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 150000002605 large molecules Chemical class 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 230000035699 permeability Effects 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- 210000000434 stratum corneum Anatomy 0.000 description 3
- 238000005728 strengthening Methods 0.000 description 3
- 230000017423 tissue regeneration Effects 0.000 description 3
- 230000010415 tropism Effects 0.000 description 3
- 231100000397 ulcer Toxicity 0.000 description 3
- 230000029663 wound healing Effects 0.000 description 3
- 208000006820 Arthralgia Diseases 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 241000628997 Flos Species 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 206010023126 Jaundice Diseases 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- 208000037093 Menstruation Disturbances Diseases 0.000 description 2
- 206010027339 Menstruation irregular Diseases 0.000 description 2
- 208000008350 Pruritus Vulvae Diseases 0.000 description 2
- 244000086363 Pterocarpus indicus Species 0.000 description 2
- 235000009984 Pterocarpus indicus Nutrition 0.000 description 2
- 206010042674 Swelling Diseases 0.000 description 2
- 241000224526 Trichomonas Species 0.000 description 2
- 208000007074 Trichomonas Vaginitis Diseases 0.000 description 2
- 208000025206 Trichomonas vaginitis urogenital infection Diseases 0.000 description 2
- 206010046914 Vaginal infection Diseases 0.000 description 2
- 201000008100 Vaginitis Diseases 0.000 description 2
- 206010056530 Vulvovaginal pruritus Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 208000010668 atopic eczema Diseases 0.000 description 2
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 2
- 229960001701 chloroform Drugs 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 208000001848 dysentery Diseases 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 230000000474 nursing effect Effects 0.000 description 2
- -1 pH regulator Substances 0.000 description 2
- 244000045947 parasite Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000003381 solubilizing effect Effects 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 208000002003 vulvitis Diseases 0.000 description 2
- 206010000077 Abdominal mass Diseases 0.000 description 1
- 201000000736 Amenorrhea Diseases 0.000 description 1
- 206010001928 Amenorrhoea Diseases 0.000 description 1
- 206010002198 Anaphylactic reaction Diseases 0.000 description 1
- 201000003126 Anuria Diseases 0.000 description 1
- 208000008035 Back Pain Diseases 0.000 description 1
- 206010006784 Burning sensation Diseases 0.000 description 1
- 206010007247 Carbuncle Diseases 0.000 description 1
- 208000032544 Cicatrix Diseases 0.000 description 1
- 208000005171 Dysmenorrhea Diseases 0.000 description 1
- 206010013935 Dysmenorrhoea Diseases 0.000 description 1
- 208000012671 Gastrointestinal haemorrhages Diseases 0.000 description 1
- 208000034507 Haematemesis Diseases 0.000 description 1
- 206010024229 Leprosy Diseases 0.000 description 1
- 208000008930 Low Back Pain Diseases 0.000 description 1
- 208000019255 Menstrual disease Diseases 0.000 description 1
- 206010027514 Metrorrhagia Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000001431 Psychomotor Agitation Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 206010038743 Restlessness Diseases 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 241000246044 Sophora flavescens Species 0.000 description 1
- 241001502500 Trichomonadida Species 0.000 description 1
- 206010046788 Uterine haemorrhage Diseases 0.000 description 1
- 206010046910 Vaginal haemorrhage Diseases 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 231100000540 amenorrhea Toxicity 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 229940124350 antibacterial drug Drugs 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 102000006834 complement receptors Human genes 0.000 description 1
- 108010047295 complement receptors Proteins 0.000 description 1
- 208000027744 congestion Diseases 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229920006037 cross link polymer Polymers 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 208000001780 epistaxis Diseases 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 210000003780 hair follicle Anatomy 0.000 description 1
- 208000035861 hematochezia Diseases 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 208000007106 menorrhagia Diseases 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 201000002266 mite infestation Diseases 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000001047 pyretic effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000037387 scars Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000000115 thoracic cavity Anatomy 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 210000003905 vulva Anatomy 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/14—Quaternary ammonium compounds, e.g. edrophonium, choline
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/234—Cnidium (snowparsley)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/286—Carthamus (distaff thistle)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/533—Leonurus (motherwort)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/756—Phellodendron, e.g. corktree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/39—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/02—Drugs for genital or sexual disorders; Contraceptives for disorders of the vagina
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Communicable Diseases (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Oncology (AREA)
- Gynecology & Obstetrics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Reproductive Health (AREA)
- Inorganic Chemistry (AREA)
- Immunology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Endocrinology (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a bacteriostatic gel for restoring female ecological balance and a preparation method thereof, and relates to the technical field of bacteriostatic drugs. A bacteriostatic gel for restoring female ecological balance is mainly prepared from bacteriostatic materials; the bacteriostatic raw materials comprise ecological balance liquid and plant extracts, wherein the ecological balance liquid comprises the following raw materials in parts by weight: 4.5-5.5 parts of acetyl heptapeptide-4, 0.3-0.8 part of recombinant human collagen, 12-25 parts of plant active polysaccharide, 15-25 parts of plant active ingredients and 10-12 parts of purified water; the antibacterial raw materials also comprise the following raw materials in parts by weight: 0.05-0.1 part of benzalkonium bromide, 0.8-1.5 parts of pH regulator, 1-3 parts of gel matrix and 30-32 parts of purified water. It has the advantage of good therapeutic effect. The preparation method comprises the following steps: dissolving, extracting plant, swelling, diluting, and gelatinizing. The preparation method has the advantage of improving the treatment effect of the product.
Description
Technical Field
The invention relates to the technical field of antibacterial drugs, in particular to an antibacterial gel for restoring female ecological balance and a preparation method thereof.
Background
The gynecological antibacterial gel has effects of dispelling pathogenic wind, removing dampness, relieving itching, killing parasite, preventing putrefaction and promoting granulation, and can be used for treating symptoms caused by vulvitis, vulval ulcer, vaginitis, etc., such as vulval or vaginal congestion, swelling, burning sensation, pain, secretion increase, topical ulcer, erosion and pruritus.
The common bacteriostatic gel commonly used at present is mainly prepared from the following raw materials: plant extract, benzalkonium bromide, pH regulator, gel matrix and water. The plant extract is prepared from Chinese herbal medicines such as cortex Phellodendri, fructus Cnidii, radix Sophorae Flavescentis, Carthami flos, herba Leonuri, etc.
In view of the above-mentioned related technologies, the inventor believes that the antibacterial gel prepared by using Chinese herbal medicines as main ingredients can achieve effective antibacterial effect, but has poor effects of nourishing and moisturizing female private parts and promoting regeneration of cells in the private parts, which affects the treatment effect.
Disclosure of Invention
In order to improve the treatment effect of the gynecological bacteriostatic gel, the application provides the bacteriostatic gel for restoring the ecological balance of women and a preparation method thereof.
In a first aspect, the application provides a bacteriostatic gel for restoring female ecological balance, which adopts the following technical scheme:
a bacteriostatic gel for restoring female ecological balance is mainly prepared from bacteriostatic materials; the bacteriostatic raw materials comprise ecological balance liquid and plant extracts, wherein the ecological balance liquid comprises the following raw materials in parts by weight: 4.5-5.5 parts of acetyl heptapeptide-4, 0.3-0.8 part of recombinant human collagen, 12-25 parts of plant active polysaccharide, 15-25 parts of plant active ingredients and 10-12 parts of purified water; the plant extract is prepared by extracting a plant mixture; the plant mixture comprises the following raw materials in parts by weight: 1.5-4 parts of radix sophorae flavescentis, 1-3 parts of cortex phellodendri, 2-5 parts of fructus cnidii, 1.5-4.5 parts of salvia miltiorrhiza, 1.2-3 parts of safflower, 1.8-4.5 parts of motherwort and 2-3.5 parts of folium artemisiae argyi; the antibacterial raw materials also comprise the following raw materials in parts by weight: 0.05-0.1 part of benzalkonium bromide, 0.8-1.5 parts of pH regulator, 1-3 parts of gel matrix and 30-32 parts of purified water.
By adopting the technical scheme, the prepared product is used as the main effective component of the antibacterial gel for restoring the ecological balance of women through the matching of all components, the perfect combination of modern biotechnology and traditional Chinese medicine technology is adopted, so that the product has the interaction of large and small molecules with plant activity, the gel has excellent hydrophilic performance, and as the acetyl heptapeptide-4 molecule is in a 3D network structure, a large amount of water and biological fluid can be absorbed, the gel is similar to biological tissues, and various plant polysaccharides and plant traditional Chinese medicine small molecule active components can be cross-linked and wrapped, so that the gel has the effects of resisting bacteria, resisting trichomonad, relieving itching, activating the cell elastin activity of female private parts, promoting the tissue repair capacity and the like, and is beneficial to making the private parts healthier, more compact and more moist. The acetyl heptapeptide-4 is a heptapeptide which is induced by probiotics, is beneficial to maintaining or promoting the balance and diversity of microbial communities, can strengthen fragile skin, is beneficial to increasing probiotics in vagina, regulating immune response, strengthening cell adhesion function, enhancing the integrity of physical barriers and enabling private micro-ecological environment to be healthier, thereby improving the self defense system and barrier protection effect of private parts. The structure of the recombinant human collagen is equivalent to that of a human body, and meanwhile, the recombinant human collagen is optimized on the basis, so that the hydrophilicity and the activity of the collagen are improved, a large number of hydrophilic genes are contained, the recombinant human collagen has good film forming property, the moisture of the stratum corneum of the skin is kept, and meanwhile, the tropism guiding effect of the recombinant human collagen can guide epithelial cells to quickly enter damaged parts, the regeneration speed of the skin is effectively improved, the wound healing time is shortened, and the barrier function of the skin is recovered. According to the application, the bacteriostatic gel is added with abundant plant extracts, so that the function of the bacteriostatic gel is richer; acetyl heptapeptide-4, benzalkonium bromide and recombinant human collagen are added into the antibacterial gel, the benzalkonium bromide is a cationic surfactant type broad-spectrum bactericide, has the effects of cleaning, sterilizing, disinfecting and killing algae, and can be used in the fields of sterilization, disinfection and corrosion prevention, the benzalkonium bromide can change the permeability of a bacterial cytoplasmic membrane, so that bacterial cytoplasmic substances are leaked out, the metabolism of the bacterial cytoplasmic substances is hindered, the killing effect is realized, and the antibacterial effect of the product is improved by adding the benzalkonium bromide into the antibacterial gel; the acetyl heptapeptide-4 and the recombinant human collagen are helpful for uniformly dispersing all components in the antibacterial gel in the product, better improving the antibacterial effect of the product and helping to improve the treatment effect of the product.
Preferably, the ecological balance liquid comprises the following raw materials in parts by weight: 4.5-5.5 parts of acetyl heptapeptide-4, 0.45-0.65 part of recombinant human collagen, 17-20 parts of plant active polysaccharide, 15-25 parts of plant active ingredients and 10-12 parts of purified water; the plant mixture comprises the following raw materials in parts by weight: 2.5-3 parts of radix sophorae flavescentis, 1-3 parts of golden cypress, 2-5 parts of fructus cnidii, 1.5-4.5 parts of salvia miltiorrhiza, 1.8-2.4 parts of safflower, 2.8-3.5 parts of motherwort and 2-3.5 parts of folium artemisiae argyi; the antibacterial raw materials also comprise the following raw materials in parts by weight: 0.05-0.1 part of benzalkonium bromide, 1-1.3 parts of pH regulator, 1.5-2.5 parts of gel matrix and 30-32 parts of purified water.
By adopting the technical scheme, the better raw material feeding proportion is used, the product antibacterial effect is favorably improved, and the product market popularization is favorably realized.
Preferably, the pH regulator is triethanolamine.
By adopting the technical scheme, the pH value of the product is adjusted by using triethanolamine, so that the product is in a proper acid-base environment, the antibacterial effect is favorably exerted, and the treatment effect of the product is favorably improved.
Preferably, the gel matrix is carbomer.
By adopting the technical scheme, the carbomer is a polyacrylic acid cross-linked polymer, a high-viscosity gel can be formed at a low concentration, and the carbomer is used for helping the effective components to be uniformly dispersed in the gel, so that the carbomer can better play a role and improve the treatment effect.
Preferably, the plant active polysaccharide consists of angelica polysaccharide and schizophyllan, and the weight ratio of the angelica polysaccharide to the schizophyllan is (0.8-1.1): 1.
By adopting the technical scheme, the angelica polysaccharide can regulate the activity of a complement receptor of a lymphocyte membrane phase, is beneficial to enhancing lymphocyte immune response and has certain enhancement effect on the immune function of an organism, and also has the effects of resisting aging, injury and radiation; the schizophyllan has the physiological activities of inhibiting tumor, improving immunity, resisting bacteria, diminishing inflammation, delaying aging and the like, and also can enhance delayed skin anaphylactic reaction, inhibit tumor growth and improve cellular immunity; by adding the plant active polysaccharide consisting of the angelica polysaccharide and the schizophyllan, the bacteriostatic effect of the product is improved, and the recovery of patients is accelerated.
Preferably, the plant active ingredients comprise the following raw materials in parts by weight: 5-8 parts of pterocarpus santalinus essential oil, 4.5-10.5 parts of lavender essential oil and 5.5-6.5 parts of purified water.
By adopting the technical scheme, the pterocarpus santalinus essential oil has a certain antibacterial effect, is beneficial to improving skin itching and inflammation, has the effect of promoting skin cell growth, can quickly recover wounds or scars, further has the effects of elasticity and contraction, is beneficial to balancing and softening skin and weakening textures, and is beneficial to rehabilitation of patients; the lavender essential oil can clear away heat and toxic materials, remove wrinkles, tender skin, promote regeneration and recovery of damaged tissues, quickly permeate hair follicles, disinfect and resist bacteria, promote cell regeneration, promote blood circulation, relieve congestion and swelling, enhance immunity and organism vitality, and can be used in the treatment process of diseases such as irregular menstruation, dysmenorrheal and the like; the use of plant active ingredients consisting of rosewood essential oil and lavender essential oil helps to improve the therapeutic effect of the product.
Preferably, the ecological balance liquid also comprises 10-12 parts by weight of collagen peptide.
By adopting the technical scheme, the collagen peptide is a fibrous high-molecular protein peptide twisted into a spiral by 3 peptide chains, can be crosslinked in the hydrogel, has good biocompatibility, can keep the inner wall of the vagina firm and elastic, is beneficial to keeping the moisture of private parts, increasing the elasticity and preventing relaxation and aging, and is beneficial to improving the treatment effect of products by adding the collagen peptide.
In a second aspect, the application provides a preparation method of a bacteriostatic gel for restoring female ecological balance, which adopts the following technical scheme:
a preparation method of a bacteriostatic gel for restoring the ecological balance of women comprises the following steps:
s1 dissolution: adding purified water 8-12 times the weight of benzalkonium bromide into benzalkonium bromide, and dissolving benzalkonium bromide to obtain antibacterial solution;
s2 plant extraction: pulverizing dried plant mixture into coarse powder, soaking in 55% ethanol solution (mass fraction, water) as solvent for 5-7 days, adding 3 times of ethanol solution, and percolating according to percolation method (Chinese pharmacopoeia) under the items of fluid extract and extract; filtering, adding 3 times of ethanol solution, soaking for 3-5 days, percolating, squeezing residue, mixing extractive solutions, decolorizing with active carbon, standing for 24 hr, filtering to remove active carbon, rotary evaporating filtrate to recover ethanol, and evaporating until the material density is 1.08-1.10g/ml (60 deg.C) to obtain plant extract;
s3 swelling: dissolving gel matrix in purified water 8-10 times of the weight of the gel matrix, standing for 10-12 hr to swell the gel matrix, and stirring to obtain swelling solution;
s4 dilution: diluting the plant active ingredient with 0.3-0.5 times of ethanol to obtain plant active diluent, and adding the rest raw materials to obtain ecological balance solution;
s5 gelling: adding the bacteriostatic solution prepared in the step S1, the plant extract prepared in the step S2 and the ecological balance solution prepared in the step S4 into the swelling solution prepared in the step S4, uniformly mixing, adding the rest purified water, stirring, adding a pH regulator to obtain a system pH value of 4-6, adding a Tween 80 emulsifier which is 0.01-0.02 time of the weight of the ecological balance solution, and uniformly mixing to obtain the bacteriostatic gel for reducing the ecological balance of the female.
By adopting the technical scheme, the plant extraction stock solution decoloration technology is adopted, no harmful chemical component and hormone are added, candida albicans, escherichia coli and staphylococcus aureus can be effectively inhibited, no inhibiting effect on beneficial bacteria lactobacillus in the vagina is generated, and the plant extraction stock solution decoloration agent can be used for nursing, removing peculiar smell and cleaning female private parts for a long time; the preparation method disclosed by the application is used for improving the treatment effect of the product.
In summary, the invention includes at least one of the following beneficial technical effects:
1. according to the application, through the matching of all components, the product is used as a main effective component of the antibacterial gel for restoring the ecological balance of women, the interaction of large and small molecules with plant activity is realized through the perfect combination of modern biotechnology and traditional Chinese medicine technology, the gel has excellent hydrophilic performance, and as the acetyl heptapeptide-4 molecule is in a 3D network structure, a large amount of water and biological fluid can be absorbed, the gel is similar to biological tissues, and various plant polysaccharides and plant traditional Chinese medicine small molecule active components can be cross-linked and wrapped, so that the gel has the effects of resisting bacteria, resisting trichomonas, relieving itching, activating the cell elastin activity of female private parts, promoting the tissue repair capacity and the like, and is beneficial to making the private parts healthier, more compact and more moist; the acetyl heptapeptide-4 is a heptapeptide which is induced by probiotics, is beneficial to maintaining or promoting the balance and diversity of microbial communities, can strengthen fragile skin, is beneficial to increasing probiotics in vagina, regulating immune response, strengthening cell adhesion function and enhancing the integrity of physical barriers, enables private micro-ecological environment to be healthier, and further improves the self defense system and barrier protection effect of private parts; the structure of the recombinant human collagen is consistent with that of a human body, and meanwhile, the recombinant human collagen is optimized on the basis, so that the hydrophilicity and the activity of the collagen are improved, a large number of hydrophilic genes are contained, the recombinant human collagen has good film-forming property, the moisture of the stratum corneum of the skin is kept, and meanwhile, the tropism guide effect of the recombinant human collagen can guide epithelial cells to quickly enter the damaged part, the regeneration speed of the skin is effectively improved, the wound healing time is shortened, and the barrier function of the skin is recovered; according to the application, the bacteriostatic gel is added with abundant plant extracts, so that the function of the bacteriostatic gel is richer; acetyl heptapeptide-4, benzalkonium bromide and recombinant human collagen are added into the antibacterial gel, the benzalkonium bromide is a cationic surfactant type broad-spectrum bactericide, has the effects of cleaning, sterilizing, disinfecting and killing algae, and can be used in the fields of sterilization, disinfection and corrosion prevention, the benzalkonium bromide can change the permeability of a bacterial cytoplasmic membrane, so that bacterial cytoplasmic substances are leaked out, the metabolism of the bacterial cytoplasmic substances is hindered, the killing effect is realized, and the antibacterial effect of the product is improved by adding the benzalkonium bromide into the antibacterial gel; the acetyl heptapeptide-4 and the recombinant human collagen are helpful for uniformly dispersing all components in the antibacterial gel in the product, so that the antibacterial effect of the product is better improved, and the treatment effect is improved;
2. the application adopts the modes of adding collagen peptide, selecting proper gel matrix, selecting plant active polysaccharide containing angelica polysaccharide and schizophyllan, selecting plant active ingredients containing rosewood essential oil and lavender essential oil and the like, is beneficial to improving the treatment effect and is beneficial to market popularization of products;
3. the application adopts a plant extraction stock solution decoloring technology, does not add harmful chemical components and hormones, can effectively inhibit candida albicans, escherichia coli and staphylococcus aureus, does not inhibit beneficial bacteria lactobacillus in the vagina, and can be used for nursing, removing peculiar smell and cleaning female private parts for a long time.
Detailed Description
Examples
The antibacterial gel is mainly used for treating gynecological diseases such as vulvitis, vulval ulcer and vaginitis, benzalkonium bromide and Chinese herbal medicines are usually added into the antibacterial gel, and the antibacterial gel prepared by taking the Chinese herbal medicines as main components can achieve an effective antibacterial effect, but has poor effects of nourishing and moisturizing female private parts and promoting the renewal of private parts cells, so that the treatment effect is influenced. The antibacterial gel is matched with each component, the product is used as a main effective component of the antibacterial gel for restoring female ecological balance, the modern biotechnology and the traditional Chinese medicine technology are perfectly combined, the product has the interaction of large and small molecules with plant activity, the gel has excellent hydrophilic performance, and because the acetyl heptapeptide-4 molecule is in a 3D network structure, a large amount of water and biological fluid can be absorbed, the antibacterial gel is similar to biological tissues, various plant polysaccharides and small molecule active components of the plant traditional Chinese medicines can be cross-linked and wrapped, the gel has the effects of resisting bacteria, resisting trichomonas, relieving itching, activating the activity of cell elastin in female private parts, promoting the tissue repair capacity and the like, and is beneficial to making the private parts healthier, more compact and more moist. The acetyl heptapeptide-4 is a heptapeptide which is induced by probiotics, is beneficial to maintaining or promoting the balance and diversity of microbial communities, can strengthen fragile skin, is beneficial to increasing probiotics in vagina, regulating immune response, strengthening cell adhesion function, enhancing the integrity of physical barriers and enabling private micro-ecological environment to be healthier, thereby improving the self defense system and barrier protection effect of private parts. The structure of the recombinant human collagen is consistent with that of a human body, and meanwhile, the recombinant human collagen is optimized on the basis, so that the hydrophilicity and the activity of the collagen are improved, a large number of hydrophilic genes are contained, the recombinant human collagen has good film forming property, the moisture of the stratum corneum of the skin is kept, meanwhile, the tropism guiding effect of the recombinant human collagen can guide epithelial cells to quickly enter the damaged part, the regeneration speed of the skin is effectively improved, the wound healing time is shortened, and the barrier function of the skin is recovered. According to the application, the bacteriostatic gel is added with abundant plant extracts, so that the function of the bacteriostatic gel is richer; acetyl heptapeptide-4, benzalkonium bromide and recombinant human collagen are added into the antibacterial gel, the benzalkonium bromide is a cationic surfactant type broad-spectrum bactericide, has the effects of cleaning, sterilizing, disinfecting and killing algae, and can be used in the fields of sterilization, disinfection and corrosion prevention, the benzalkonium bromide can change the permeability of a bacterial cytoplasmic membrane, so that bacterial cytoplasmic substances are leaked out, the metabolism of the bacterial cytoplasmic substances is hindered, the killing effect is realized, and the antibacterial effect of the product is improved by adding the benzalkonium bromide into the antibacterial gel; the acetyl heptapeptide-4 and the recombinant human collagen are beneficial to improving the stability of benzalkonium bromide in the antibacterial gel, and are beneficial to uniformly dispersing all components in the antibacterial gel in products, so that the antibacterial effect of the products is better improved, and the treatment effect of the products is improved.
The application uses abundant plant extracts, and the sophora flavescens extract has the effects of clearing heat and drying dampness, killing parasites and promoting urination, and can be used for treating dysentery with fever, hematochezia, jaundice anuria, leucorrhea with reddish discharge, vulva swelling and pruritus vulvae, eczema, skin pruritus, mange and leprosy and externally treating trichomonas vaginitis. Cortex Phellodendri extract can be used for treating damp-heat dysentery, jaundice with dark urine, leukorrhagia pruritus vulvae, pyretic stranguria with pain, pyocutaneous disease, toxic swelling, and eczema. The fructus Cnidii extract can be used for treating dampness eliminating, pathogenic wind dispelling, uterus cold, leukorrhagia due to cold-dampness, lumbago due to damp arthralgia, vulval eczema, pudendal pruritus of women, and trichomonas vaginitis. The Saviae Miltiorrhizae radix extract has effects of promoting blood circulation, removing blood stasis, dredging channels, relieving pain, clearing heart fire, relieving restlessness, cooling blood, and resolving carbuncle, and can be used for treating thoracic obstruction, heart pain, abdominal mass, heat arthralgia, vexation, insomnia, menoxenia, dysmenorrhea, amenorrhea and pyocutaneous disease with swelling and pain. The Carthami flos extract has effects of promoting blood circulation, dredging channels, removing blood stasis, relieving pain, and promoting blood circulation, removing blood stasis, and eliminating dampness. The folium Artemisiae Argyi extract can be used for treating hematemesis, epistaxis, metrorrhagia, menorrhagia, vaginal bleeding due to fetal leakage, lower abdomen psychroalgia and irregular menstruation, has excellent antibacterial effect, and can be used for treating skin pruritus.
The raw materials related to the invention are all commercially available, and the types and sources of the raw materials are shown in table 1.
TABLE 1 Specification, type and origin of the raw materials
The plant medicinal materials used in the plant extracts are all produced in Shaanxi and can be used after being dried.
Example 1: a preparation method of bacteriostatic gel for restoring female ecological balance comprises the following steps:
s1 dissolution: 0.08kg of benzalkonium bromide is taken, 0.8kg of purified water (the conductivity is 12 mu s/cm) is added, the mixture is stirred uniformly, and the benzalkonium bromide is dissolved to prepare the antibacterial solution.
S2 plant extraction: adding water into absolute ethyl alcohol to prepare 55 percent (mass concentration) of ethanol solution. Taking 2.8kg of dried radix sophorae flavescentis, adding 2kg of cortex phellodendri, 3.5kg of fructus cnidii, 3kg of radix salviae miltiorrhizae, 2.1kg of safflower, 3.2kg of motherwort and 2.8kg of folium artemisiae argyi, uniformly mixing to obtain a plant mixture, crushing the plant mixture by using a crusher to obtain coarse powder with the particle size of not more than 2.5mm, soaking the plant mixture for 6 days at one time by using 55% ethanol solution as a solvent according to a percolation method (Chinese pharmacopoeia) under the items of fluid extract and extract, and slowly percolating the plant mixture; filtering, adding 49.8kg ethanol solution into the solid, soaking for 4 days, slowly percolating, squeezing the residue, mixing the two extractive solutions, mixing, decolorizing with active carbon, standing for 24 hr, filtering to remove active carbon, rotary evaporating the filtrate at 60 deg.C to recover ethanol, and evaporating until the material density is 1.09g/ml (60 deg.C) to obtain plant extract.
S3 swelling: dissolving 2kg of carbomer in 18kg of purified water, standing for 11 hours, swelling the gel matrix, and uniformly stirring to obtain a swelling solution.
S4 dilution: taking 7.5kg of lavender essential oil, adding 6.5kg of pterocarpus santalinus essential oil and 6kg of purified water, adding 8kg of absolute ethyl alcohol for dilution to prepare a plant active diluent, adding 11kg of collagen peptide, 0.55kg of recombinant human collagen, 8.8kg of angelica polysaccharide, 9.2kg of schizophyllan, 5kg of acetyl heptapeptide-4 and 11kg of purified water into the plant active diluent, and uniformly mixing to prepare the ecological balance liquid.
S5 gelling: adding the antibacterial liquid prepared in the step S1, the plant extract prepared in the step S2 and the ecological balance liquid prepared in the step S4 into the swelling liquid prepared in the step S4, uniformly mixing, adding the rest 12.2kg of purified water, stirring at the rotating speed of 200 revolutions per minute, slowly adding 1.2kg of triethanolamine to adjust the pH value of the system to be 5, adding 1.1kg of Tween 80 emulsifier, uniformly mixing, and gelling to prepare the antibacterial gel for reducing the ecological balance of the female.
Example 2
Example 2 differs from example 1 in that example 2 does not add collagen peptide and otherwise remains the same as example 1.
Examples 3 to 10
Examples 3 to 10 differ from example 1 in the amount of each ingredient added in examples 3 to 10, the weight ratio of purified water used for solubilizing benzalkonium bromide to benzalkonium bromide was kept the same as in example 1, the weight ratio of purified water used for solubilizing carbomer to carbomer was kept the same as in example 1, and the other examples were kept the same as in example 1, and the amounts of each ingredient added in examples 3 to 10 are shown in Table 2.
TABLE 2 addition amounts of the respective raw materials of examples 3 to 10
Examples 11 to 14
Examples 11-14 differ from example 1 in that the process parameters for each step of examples 11-14 are different, the total purified water usage of examples 11-14 is consistent with example 1, and the process parameters for each step of examples 11-14 are shown in Table 3.
TABLE 3 parameters in the various steps of examples 11-14
Comparative example
Comparative example 1
Comparative example 1 is different from example 1 in that comparative example 1 does not add acetyl heptapeptide-4 and recombinant human collagen, and the others are identical to example 1.
Performance detection
1. Benzalkonium bromide stability: the stability of benzalkonium bromide needs to be tested; the stability experiments were as follows: putting the product into a constant temperature and humidity box, standing for 90 days at 38 ℃ and 75% relative humidity; and (3) measuring the content of benzalkonium bromide in the product before and after the stability experiment, and dividing the difference value of the content of benzalkonium bromide before and after the stability experiment by the content of benzalkonium bromide before the stability experiment to obtain the reduction rate of benzalkonium bromide. The method for testing the content of benzalkonium bromide comprises the following steps: taking 200g of bacteriostatic gel, adding 50ml of distilled water and 1ml of sodium hydroxide aqueous solution with the mass concentration of 4.3%, shaking up, adding 0.4ml of bromophenol blue indicator solution (prepared from 0.1g of bromophenol blue, 3ml of 0.05mol/L sodium hydroxide aqueous solution and distilled water till 200 ml) and 10ml of trichloromethane, shaking up, titrating with 0.02mol/L sodium tetraphenylborate titration solution, shaking up forcefully until the blue color of a trichloromethane layer disappears, calculating the content of benzalkonium bromide in the bacteriostatic gel, and then calculating the reduction rate of the benzalkonium bromide. The results are shown in Table 4.
2. The bacteriostatic effect is as follows: standing the product at room temperature for 40 days, and detecting the antibacterial effect; the detection method refers to GB15979-2002 hygienic Standard for Disposable sanitary articles, and detects average inhibition rate of Escherichia coli, Staphylococcus aureus and Candida albicans after 2min, 5min, 10min and 20 min. The results are shown in tables 5-7.
TABLE 4 comparison of benzalkonium bromide stability test results in different antibacterial gel products
| Sample numbering | Benzalkonium bromide decreasing rate (%) |
| Example 1 | 1.01 |
| Example 2 | 1.57 |
| Example 3 | 1.63 |
| Example 4 | 1.24 |
| Example 5 | 1.28 |
| Example 6 | 1.36 |
| Example 7 | 1.29 |
| Example 8 | 1.14 |
| Example 9 | 1.07 |
| Example 10 | 1.12 |
| Example 11 | 1.02 |
| Example 12 | 0.96 |
| Example 13 | 0.91 |
| Example 14 | 1.03 |
| Comparative example 1 | 4.73 |
TABLE 5 comparison of the inhibition effect of different bacteriostatic gel products on escherichia coli
TABLE 6 comparison of inhibitory effect of different bacteriostatic gel products on staphylococcus aureus
TABLE 7 comparison of the inhibitory effect of different bacteriostatic gel products on Candida albicans
The lower the reduction rate of the benzalkonium bromide is, the better the stability of the benzalkonium bromide is, and the better the antibacterial effect of the product is.
Compared with the prior art, the acetyl heptapeptide-4 and the recombinant human collagen are not added in the comparative example 1, so that the benzalkonium bromide in the prepared antibacterial gel product has poor stability and low antibacterial efficiency, and is not beneficial to product market popularization.
Comparing the experimental results of the example 1 and the comparative example 1, it can be seen that, in the process of preparing the antibacterial gel, the acetyl heptapeptide-4 and the recombinant human collagen are added simultaneously, so that the stability of benzalkonium bromide in the prepared antibacterial gel product is obviously improved, the antibacterial efficiency of the product is obviously improved, and the market popularization of the product is facilitated.
Comparing the experimental results of example 1 and example 2, the collagen peptide is not added in example 2, and the stability of benzalkonium bromide in the prepared bacteriostatic gel product is slightly reduced, and the bacteriostatic efficiency is slightly reduced, which is not beneficial to the market popularization of the product.
Compared with the embodiment 1, the addition amounts of the raw materials in the embodiments 3 to 10 are different, the process parameters in the steps of the embodiments 11 to 14 are different, the stability of benzalkonium bromide in the prepared antibacterial gel product is good, the product has excellent antibacterial effects on escherichia coli, candida albicans and staphylococcus aureus, and the market popularization of the product is facilitated.
The embodiments of the present invention are preferred embodiments of the present invention, and the scope of the present invention is not limited by these embodiments, so: all equivalent changes made according to the structure, shape and principle of the invention are covered by the protection scope of the invention.
Claims (8)
1. An antibacterial gel for restoring the ecological balance of women is characterized by being mainly prepared from antibacterial raw materials; the bacteriostatic raw materials comprise ecological balance liquid and plant extracts, wherein the ecological balance liquid comprises the following raw materials in parts by weight: 4.5-5.5 parts of acetyl heptapeptide-4, 0.3-0.8 part of recombinant human collagen, 12-25 parts of plant active polysaccharide, 15-25 parts of plant active ingredients and 10-12 parts of purified water; the plant extract is prepared by extracting a plant mixture; the plant mixture comprises the following raw materials in parts by weight: 1.5-4 parts of radix sophorae flavescentis, 1-3 parts of cortex phellodendri, 2-5 parts of fructus cnidii, 1.5-4.5 parts of salvia miltiorrhiza, 1.2-3 parts of safflower, 1.8-4.5 parts of motherwort and 2-3.5 parts of folium artemisiae argyi; the antibacterial raw materials also comprise the following raw materials in parts by weight: 0.05-0.1 part of benzalkonium bromide, 0.8-1.5 parts of pH regulator, 1-3 parts of gel matrix and 30-32 parts of purified water.
2. The bacteriostatic gel for restoring female ecological balance according to claim 1, wherein the ecological balance liquid comprises the following raw materials in parts by weight: 4.5-5.5 parts of acetyl heptapeptide-4, 0.45-0.65 part of recombinant human collagen, 17-20 parts of plant active polysaccharide, 15-25 parts of plant active ingredients and 10-12 parts of purified water; the plant mixture comprises the following raw materials in parts by weight: 2.5-3 parts of radix sophorae flavescentis, 1-3 parts of golden cypress, 2-5 parts of fructus cnidii, 1.5-4.5 parts of salvia miltiorrhiza, 1.8-2.4 parts of safflower, 2.8-3.5 parts of motherwort and 2-3.5 parts of folium artemisiae argyi; the antibacterial raw materials also comprise the following raw materials in parts by weight: 0.05-0.1 part of benzalkonium bromide, 1-1.3 parts of pH regulator, 1.5-2.5 parts of gel matrix and 30-32 parts of purified water.
3. The bacteriostatic gel for restoring female ecological balance according to claim 1, characterized in that: the pH regulator is triethanolamine.
4. The bacteriostatic gel for restoring female ecological balance according to claim 3, characterized in that: the gel matrix is carbomer.
5. The bacteriostatic gel for restoring female ecological balance according to claim 1, characterized in that: the plant active polysaccharide consists of angelica polysaccharide and schizophyllan, and the weight ratio of the angelica polysaccharide to the schizophyllan is (0.8-1.1): 1.
6. The bacteriostatic gel for restoring female ecological balance according to claim 1, characterized in that: the plant active ingredients comprise the following raw materials in parts by weight: 5-8 parts of pterocarpus santalinus essential oil, 4.5-10.5 parts of lavender essential oil and 5.5-6.5 parts of purified water.
7. The bacteriostatic gel for restoring female ecological balance according to claim 1, characterized in that: the ecological balance liquid also comprises 10-12 parts by weight of collagen peptide.
8. A method of preparing a bacteriostatic gel for restoring female ecological balance according to any one of claims 1 to 7, comprising the steps of:
s1 dissolution: adding purified water 8-12 times the weight of benzalkonium bromide into benzalkonium bromide, and dissolving benzalkonium bromide to obtain antibacterial solution;
s2 plant extraction: pulverizing dried plant mixture into coarse powder, soaking in 55% ethanol solution (mass fraction, water) as solvent for 5-7 days, adding 3 times of ethanol solution, and percolating according to percolation method (Chinese pharmacopoeia) under the items of fluid extract and extract; filtering, adding 3 times of ethanol solution, soaking for 3-5 days, percolating, squeezing residue, mixing extractive solutions, decolorizing with active carbon, standing for 24 hr, filtering to remove active carbon, rotary evaporating filtrate to recover ethanol, and evaporating until the material density is 1.08-1.10g/ml (60 deg.C) to obtain plant extract;
s3 swelling: dissolving gel matrix in purified water 8-10 times of the weight of the gel matrix, standing for 10-12 hr to swell the gel matrix, and stirring to obtain swelling solution;
s4 dilution: diluting the plant active ingredient with 0.3-0.5 times of ethanol to obtain plant active diluent, and adding the rest raw materials to obtain ecological balance solution;
s5 gelling: and (3) adding the bacteriostatic solution prepared in the step (S1), the plant extract prepared in the step (S2) and the ecological balance liquid prepared in the step (S4) into the swelling liquid prepared in the step (S4), uniformly mixing, adding the rest purified water, stirring, adding a pH regulator, adding a Tween 80 emulsifier which is 0.01-0.02 time of the weight of the ecological balance liquid, and uniformly mixing to prepare the bacteriostatic gel for restoring the ecological balance of the female.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011314033.5A CN112370489A (en) | 2020-11-21 | 2020-11-21 | Antibacterial gel for restoring ecological balance of women and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011314033.5A CN112370489A (en) | 2020-11-21 | 2020-11-21 | Antibacterial gel for restoring ecological balance of women and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112370489A true CN112370489A (en) | 2021-02-19 |
Family
ID=74587245
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011314033.5A Pending CN112370489A (en) | 2020-11-21 | 2020-11-21 | Antibacterial gel for restoring ecological balance of women and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112370489A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114712482A (en) * | 2022-04-09 | 2022-07-08 | 朵博士健康科技(杭州)有限公司 | Vagina-cleaning bacteriostatic gel containing astaxanthin and preparation method thereof |
| CN116003577A (en) * | 2023-02-14 | 2023-04-25 | 广东丸美生物技术股份有限公司 | Recombinant collagen for skin soothing and repairing and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110693778A (en) * | 2019-10-29 | 2020-01-17 | 浙江英树生物科技有限公司 | Face cream and production process thereof |
| CN111135292A (en) * | 2020-01-17 | 2020-05-12 | 赵丽 | Recombinant private collagen peptide nursing antibacterial gel and preparation method thereof |
| CN111358939A (en) * | 2020-03-17 | 2020-07-03 | 陕西健驰生物药业有限公司 | Traditional Chinese medicine preparation for promoting female genital tract repair and preparation method thereof |
-
2020
- 2020-11-21 CN CN202011314033.5A patent/CN112370489A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110693778A (en) * | 2019-10-29 | 2020-01-17 | 浙江英树生物科技有限公司 | Face cream and production process thereof |
| CN111135292A (en) * | 2020-01-17 | 2020-05-12 | 赵丽 | Recombinant private collagen peptide nursing antibacterial gel and preparation method thereof |
| CN111358939A (en) * | 2020-03-17 | 2020-07-03 | 陕西健驰生物药业有限公司 | Traditional Chinese medicine preparation for promoting female genital tract repair and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 于玲，等: "冰硼散联合中药熏洗治疗复发性念珠菌性阴道炎35例", 《陕西中医》 * |
| 陕西远志医药生物工程有限公司，著: "芳茵R多肽护理抑菌凝胶", 《Q/SXYZ 001-2020芳茵R多肽护理抑菌凝胶》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114712482A (en) * | 2022-04-09 | 2022-07-08 | 朵博士健康科技(杭州)有限公司 | Vagina-cleaning bacteriostatic gel containing astaxanthin and preparation method thereof |
| CN114712482B (en) * | 2022-04-09 | 2024-06-25 | 朵博士健康科技(杭州)有限公司 | Astaxanthin-containing vagina cleaning antibacterial gel and preparation method thereof |
| CN116003577A (en) * | 2023-02-14 | 2023-04-25 | 广东丸美生物技术股份有限公司 | Recombinant collagen for skin soothing and repairing and application thereof |
| CN116003577B (en) * | 2023-02-14 | 2023-10-31 | 广东丸美生物技术股份有限公司 | Recombinant collagen for skin soothing and repairing and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109078165B (en) | Composition for nursing female private parts and preparation method and application thereof | |
| CN103920072B (en) | A kind of chitosan antibacterial gel and preparation method thereof | |
| CN101879283B (en) | Novel membrane spraying preparation for skin trauma and preparation method | |
| CN113081928B (en) | Plant antibacterial gel and preparation method and application thereof | |
| CN112353866A (en) | Polypeptide nursing antibacterial gel and preparation method thereof | |
| CN111744054B (en) | Antibacterial wound surface dressing capable of promoting healing and preparation method thereof | |
| CN103272153A (en) | Antibacterial gel and preparation method thereof | |
| CN101269192A (en) | Chinese medicinal composition for treating gynaecologic inflammation and preparation thereof | |
| CN106214573A (en) | A kind of medical disposable hand saver skin disinfectant solution and preparation method thereof | |
| CN104688786B (en) | A kind of externally-applied medicinal composition and its preparation method and application | |
| CN112370489A (en) | Antibacterial gel for restoring ecological balance of women and preparation method thereof | |
| CN103893640B (en) | A kind of chitosan infant bio-medical antimicrobial fluid and preparation method thereof | |
| CN104547303A (en) | Externally used gel for treating gynecological inflammation and preparation method thereof | |
| CN111803624A (en) | Skin antibacterial gel preparation containing transfer factor and preparation method thereof | |
| CN111297961A (en) | Dong medicine for promoting wound healing | |
| CN113616714A (en) | High-selenium anti-HPV (human papillomavirus) biological protein gel dressing and preparation method thereof | |
| CN101053612B (en) | External applied traditional Chinese medicine vagina cleaning agent for curing gynecopathy and vagina cleaning ointment thereof | |
| CN105012818A (en) | Composition for treating gynecological inflammation and preparation and preparation method thereof | |
| CN103599437B (en) | One treats colpitic traditional Chinese medicine liquid | |
| CN108653402A (en) | giant knotweed skin antibacterial liquid | |
| CN107243029B (en) | Bacteriostatic lotion and preparation method and application thereof | |
| CN112546113A (en) | Probiotic bacteriostatic gel and preparation method thereof | |
| CN105106459A (en) | Traditional Chinese medicine gelling agent for treating superficial ulcer stage pressure sores and preparation method thereof | |
| CN112472772A (en) | Antibacterial gel and preparation method thereof | |
| CN111184832A (en) | Plant probiotic composition with functions of contracting vagina, lubricating, nourishing and protecting nests and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210219 |
|
| RJ01 | Rejection of invention patent application after publication |