CN106260642A - A kind of lactobacillus feed additive preventing and treating swine fever and its preparation method and application - Google Patents
A kind of lactobacillus feed additive preventing and treating swine fever and its preparation method and application Download PDFInfo
- Publication number
- CN106260642A CN106260642A CN201610671903.1A CN201610671903A CN106260642A CN 106260642 A CN106260642 A CN 106260642A CN 201610671903 A CN201610671903 A CN 201610671903A CN 106260642 A CN106260642 A CN 106260642A
- Authority
- CN
- China
- Prior art keywords
- parts
- feed additive
- swine fever
- preventing
- lactobacillus feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/734—Crataegus (hawthorn)
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of lactobacillus feed additive preventing and treating swine fever and its preparation method and application, this lactobacillus feed additive is made up of according to the raw material of weight portion following: lactic acid bacteria 15 parts, wood root 8 16 parts, Herba thalictri squarrosi 5 12 parts, Cortex Glyptostrobi Pensilis 10 18 parts, Folium Sapii 15 23 parts, Fructus Crataegi 20 28 parts.Wood root, Cortex Glyptostrobi Pensilis are pulverized, adds deionized water heat treated, filter to get filtrate；Herba thalictri squarrosi, Folium Sapii, Fructus Crataegi being pulverized, addition vinegar seals immersion, stir process prepares mixed material；After filtrate, mixed material are down to room temperature, be incorporated in 35 DEG C at a temperature of mixed with lactic acid bacteria is fermented, and stirring is to doing and get final product.The present invention has definite efficacy result notable for preventing and treating hog cholera, instant effect, without any side effects, pollution-free, no drug residue, have no drug resistance, non-relapse after healing, treating both the principal and secondary aspects of a disease, pig is searched for food without negative effect, and consumption is few, promotes pig weightening finish, and make its product meat reach nuisanceless requirement.
Description
Technical field
The present invention relates to field of feed additive technology for animals, a kind of lactobacillus feed additive preventing and treating swine fever
And its preparation method and application.
Background technology
Swine fever is commonly called as " rinderpest ", be the one that the swine fever virus belonged to by flaviviridae swine fever virus causes acute, heating,
Contagious infection infectious disease.It is one of Infectious Diseases threatening pig industry, it is characterized in that: acute, change in septic, essence
Organ hemorrhage, downright bad and infarction；Chronic in cellulosic necrotic enteritis, often there are paratyphoid fever and Bacillus pasteurii disease secondary in the later stage.
There is hyperinfection and lethal.A primary disease infected pigs under field conditions (factors), all ages and classes, sex, the pig of kind and wild boar
The most susceptible, the most all can occur.Sick pig is major source of infection, sick pig excrements and secretions, sick dead pig and internal organs and corpse
Body, anxious the government official sick blood of pig, meat, internal organs, waste water, the feedstuff of waste entrainment, drinking-water all can disseminate virus, and the propagation of swine fever is mainly led to
Cross contact, through digestive tract infection.Additionally, ill and low virulent strain infection sow can also produce through Placenta Hominis vertical infection fetus
Weak piglet, stillborn fetus, mummy tire etc..
At present, treating this disease and be most commonly used that Chinese medicine and antiviral Western medicine, Chinese medicine is owing to its prescription is unreasonable or simply letter
Single pulverizing, particle diameter is excessive, and drug release is relatively slow, causes its bioavailability relatively low, and clinical effectiveness is not fine；And it is various anti-
The virus medicine such as Ministry of Agriculture such as ribavirin, amantadine has prohibitted the use of.Therefore research and develop and a kind of treat the residual without medicine of swine fever
Stay, the specific medicament produced that has no drug resistance is one of this field scientific research technical staff new problem anxious to be resolved.
Summary of the invention
It is an object of the invention to provide a kind of instant effect, without any side effects, pollution-free, no drug residue, have no drug resistance,
Cure rate height, non-relapse after healing, the lactobacillus feed additive and its preparation method and application of preventing and treating swine fever of weightening finish, to solve
The problem certainly proposed in above-mentioned background technology.
For achieving the above object, the present invention provides following technical scheme:
A kind of lactobacillus feed additive preventing and treating swine fever, is made up of according to the raw material of weight portion following: lactic acid bacteria 1-5
Part, wood root 8-16 part, Herba thalictri squarrosi 5-12 part, Cortex Glyptostrobi Pensilis 10-18 part, Folium Sapii 15-23 part, Fructus Crataegi 20-28 part.
As the further scheme of the present invention: the lactobacillus feed additive of described preventing and treating swine fever, by following according to weight
The raw material composition of part: lactic acid bacteria 2-4 part, wood root 10-14 part, Herba thalictri squarrosi 7-10 part, Cortex Glyptostrobi Pensilis 12-16 part, Folium Sapii
17-21 part, Fructus Crataegi 22-26 part.
As the further scheme of the present invention: the lactobacillus feed additive of described preventing and treating swine fever, by following according to weight
The raw material composition of part: lactic acid bacteria 3 parts, wood root 12 parts, Herba thalictri squarrosi 8 parts, Cortex Glyptostrobi Pensilis 14 parts, Folium Sapii 19 parts, Fructus Crataegi 24
Part.
Another object of the present invention is to provide the preparation method of a kind of lactobacillus feed additive preventing and treating swine fever, by following step
Rapid composition:
1) wood root, Cortex Glyptostrobi Pensilis are pulverized, crossed 100 mesh sieves, add the deionized water of the two quality 10 times, the temperature of 70 DEG C
The lower heated and stirred of degree processes 3h, then at 98-100 DEG C, heated and stirred processes 0.8-1h, filters to get filtrate；
2) Herba thalictri squarrosi, Folium Sapii, Fructus Crataegi are pulverized, cross 150 mesh sieves, add the vinegar of three's mass 0.8 times,
65-68 DEG C of lower seal soaks 3h, then stir process 60min at a temperature of 120 DEG C, prepares mixed material；
3), after filtrate, mixed material being down to room temperature, mix with lactic acid bacteria and be incorporated in fermentation 6h at a temperature of 35 DEG C, then exist
At a temperature of 50 DEG C, stirring is extremely done and is i.e. obtained lactobacillus feed additive.
Another object of the present invention is to provide the application in pig feed of the described lactobacillus feed additive.
Another object of the present invention is to provide the application in preventing and treating swine fever medicine of the described lactobacillus feed additive.
Lactic acid bacteria can promote growth of animal, regulation composition of gut flora, maintenance microecological balance, thus improves gastrointestinal tract
Function；Improve food digestion rate and biological value；Reduce serum cholesterol, control endotoxin；Putrefaction bacteria growth in suppression intestinal:
Improve immunity of organisms etc..
Wood root wind-damp dispelling, diuresis, dissipating blood stasis blood, detumescence poison.Treating rheumatic arthritis, oedema due to nephritis, cirrhotic ascites,
Acute, chronic hepatitis, stomachache, stranguria with turbid discharge, metrorrhagia, traumatic injury, scrofula, carbuncle.
Herba thalictri squarrosi heat-clearing and toxic substances removing, stomach invigorating antacid, diaphoresis.
Cortex Glyptostrobi Pensilis killing parasites for relieving itching；Releasing fire-toxin.Main blister skin ulcer；Burn due to hot liquid or fire.
Folium Sapii controls carbuncle furuncle, skin ulcer scabies, tinea pedis, eczema, snakebite, vaginitis.
Hawthorn digesting amasss, dissipating blood stasis blood, expelling tenia.Control meat to amass, lump in the abdomen, phlegm retention, feeling of fullness, acid regurgitation, dysentery, hemorrhoidal hamorrhage, lumbago, hernia
Gas after-pains in puerperal, lochiorrhea, children's's breast food is stagnated.Promoting digestion and invigorating the stomach, circulation of qi promoting dissipating blood stasis.For meat stagnation, stomach alkane distension, rush down
Dysentery stomachache, blood stasis amenorrhea, postpartum stagnation, trusted subordinate's twinge, hernia pain, hyperlipidemia.
Compared with prior art, the invention has the beneficial effects as follows:
The lactobacillus feed additive of the present invention, under the synergism of each raw material, has definite curative effect for preventing and treating hog cholera
Effect is notable, and instant effect is without any side effects, pollution-free, no drug residue, has no drug resistance, and cure rate is high, non-relapse after healing,
Treating both the principal and secondary aspects of a disease, searches for food to pig without negative effect, and consumption is few, improves pig body health, reduces the generation of disease, promotes
Pig increases weight, and is conducive to increasing the income of pig farmer, effect highly significant.The present invention improves the immunocompetence of full swinery, moreover it is possible to pre-
Anti-various diseases (swine fever, pig lung plague, viral diarrhea etc.), improve efficiency of feed utilization, and speed of weight increment is fast.Pure Chinese medicine ingredients, green
Color safety and environmental protection, and make its product meat reach nuisanceless requirement, meet the export standard of product, be effectively ensured people's
Healthy.Preparation process of the present invention is simple, and low cost is suitable to industrialized production.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, all
Belong to the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, a kind of lactobacillus feed additive preventing and treating swine fever, by the following raw material according to weight portion
Composition: lactic acid bacteria 1 part, wood root 8 parts, Herba thalictri squarrosi 5 parts, Cortex Glyptostrobi Pensilis 10 parts, Folium Sapii 15 parts, Fructus Crataegi 20 parts.
Wood root, Cortex Glyptostrobi Pensilis are pulverized, crossed 100 mesh sieves, adds the deionized water of the two quality 10 times, the temperature of 70 DEG C
Lower heated and stirred processes 3h, then at 98 DEG C, heated and stirred processes 0.8h, filters to get filtrate.By Herba thalictri squarrosi, Folium Sapii, mountain
Short, bristly hair or beard is pulverized, is crossed 150 mesh sieves, adds the vinegar of three's mass 0.8 times, soaks 3h at 65 DEG C of lower seals, then at a temperature of 120 DEG C
Stir process 60min, prepares mixed material.After filtrate, mixed material are down to room temperature, mix the temperature being incorporated in 35 DEG C with lactic acid bacteria
Degree bottom fermentation 6h, then at a temperature of 50 DEG C, stirring is extremely done and is i.e. obtained lactobacillus feed additive.
Embodiment 2
In the embodiment of the present invention, a kind of lactobacillus feed additive preventing and treating swine fever, by the following raw material according to weight portion
Composition: lactic acid bacteria 5 parts, wood root 16 parts, Herba thalictri squarrosi 12 parts, Cortex Glyptostrobi Pensilis 18 parts, Folium Sapii 23 parts, Fructus Crataegi 28 parts.
Wood root, Cortex Glyptostrobi Pensilis are pulverized, crossed 100 mesh sieves, adds the deionized water of the two quality 10 times, the temperature of 70 DEG C
Lower heated and stirred processes 3h, then at 100 DEG C, heated and stirred processes 1h, filters to get filtrate.By Herba thalictri squarrosi, Folium Sapii, mountain
Short, bristly hair or beard is pulverized, is crossed 150 mesh sieves, adds the vinegar of three's mass 0.8 times, soaks 3h at 68 DEG C of lower seals, then at a temperature of 120 DEG C
Stir process 60min, prepares mixed material.After filtrate, mixed material are down to room temperature, mix the temperature being incorporated in 35 DEG C with lactic acid bacteria
Degree bottom fermentation 6h, then at a temperature of 50 DEG C, stirring is extremely done and is i.e. obtained lactobacillus feed additive.
Embodiment 3
In the embodiment of the present invention, a kind of lactobacillus feed additive preventing and treating swine fever, by the following raw material according to weight portion
Composition: lactic acid bacteria 2 parts, wood root 10 parts, Herba thalictri squarrosi 7 parts, Cortex Glyptostrobi Pensilis 12 parts, Folium Sapii 17 parts, Fructus Crataegi 22 parts.
Wood root, Cortex Glyptostrobi Pensilis are pulverized, crossed 100 mesh sieves, adds the deionized water of the two quality 10 times, the temperature of 70 DEG C
Lower heated and stirred processes 3h, then at 99 DEG C, heated and stirred processes 0.9h, filters to get filtrate.By Herba thalictri squarrosi, Folium Sapii, mountain
Short, bristly hair or beard is pulverized, is crossed 150 mesh sieves, adds the vinegar of three's mass 0.8 times, soaks 3h at 66 DEG C of lower seals, then at a temperature of 120 DEG C
Stir process 60min, prepares mixed material.After filtrate, mixed material are down to room temperature, mix the temperature being incorporated in 35 DEG C with lactic acid bacteria
Degree bottom fermentation 6h, then at a temperature of 50 DEG C, stirring is extremely done and is i.e. obtained lactobacillus feed additive.
Embodiment 4
In the embodiment of the present invention, a kind of lactobacillus feed additive preventing and treating swine fever, by the following raw material according to weight portion
Composition: lactic acid bacteria 4 parts, wood root 14 parts, Herba thalictri squarrosi 10 parts, Cortex Glyptostrobi Pensilis 16 parts, Folium Sapii 21 parts, Fructus Crataegi 26 parts.
Wood root, Cortex Glyptostrobi Pensilis are pulverized, crossed 100 mesh sieves, adds the deionized water of the two quality 10 times, the temperature of 70 DEG C
Lower heated and stirred processes 3h, then at 99 DEG C, heated and stirred processes 0.9h, filters to get filtrate.By Herba thalictri squarrosi, Folium Sapii, mountain
Short, bristly hair or beard is pulverized, is crossed 150 mesh sieves, adds the vinegar of three's mass 0.8 times, soaks 3h at 66 DEG C of lower seals, then at a temperature of 120 DEG C
Stir process 60min, prepares mixed material.After filtrate, mixed material are down to room temperature, mix the temperature being incorporated in 35 DEG C with lactic acid bacteria
Degree bottom fermentation 6h, then at a temperature of 50 DEG C, stirring is extremely done and is i.e. obtained lactobacillus feed additive.
Embodiment 5
In the embodiment of the present invention, a kind of lactobacillus feed additive preventing and treating swine fever, by the following raw material according to weight portion
Composition: lactic acid bacteria 3 parts, wood root 12 parts, Herba thalictri squarrosi 8 parts, Cortex Glyptostrobi Pensilis 14 parts, Folium Sapii 19 parts, Fructus Crataegi 24 parts.
Wood root, Cortex Glyptostrobi Pensilis are pulverized, crossed 100 mesh sieves, adds the deionized water of the two quality 10 times, the temperature of 70 DEG C
Lower heated and stirred processes 3h, then at 99 DEG C, heated and stirred processes 0.9h, filters to get filtrate.By Herba thalictri squarrosi, Folium Sapii, mountain
Short, bristly hair or beard is pulverized, is crossed 150 mesh sieves, adds the vinegar of three's mass 0.8 times, soaks 3h at 66 DEG C of lower seals, then at a temperature of 120 DEG C
Stir process 60min, prepares mixed material.After filtrate, mixed material are down to room temperature, mix the temperature being incorporated in 35 DEG C with lactic acid bacteria
Degree bottom fermentation 6h, then at a temperature of 50 DEG C, stirring is extremely done and is i.e. obtained lactobacillus feed additive.
Comparative example 1
In addition to not containing Herba thalictri squarrosi, its formula and preparation process are consistent with embodiment 5.
Comparative example 2
Containing only Herba thalictri squarrosi, its preparation process is consistent with embodiment 5.
Embodiment 6 toxicity test
1 material and method
1.1 material
1.1.1 laboratory animal Kunming hybridization healthy mice, body weight 18～24g, be purchased from Hunan Medical University's zoopery
Portion.
1.1.2 the lactobacillus feed additive that the embodiment of the present invention 5 prepares.
1.2 method
1.2.1 acute toxicity test
Using administration by gavage to take 60 white mice, be randomly divided into six groups, often group 10, male and female half and half, every white mice presses body
Heavily it is administered and is: first group of given low is 10g/kg, and second group of given low is 20g/kg, and the 3rd group is 50g/kg, the 4th group
For 100g/kg, the 5th group is 150g/kg.Matched group gavage distilled water 150g/kg.
1.2.2 chronic toxicity test
50 white mice are randomly divided into five groups, often group 10, male and female half and half (separately feeding), gavaging of each group white mice
Dosage is calculated by the every 20g of body weight, and first group is 0.3g, and second group is 0.4g, and the 3rd group is 0.6g, and the 4th group is 0.8g.Every day
Gavaging the morning once, matched group gavages 1.00g/ distilled water every day, observe every day white mice spirit, appetite, activity, fecaluria and
Death condition.Weighing once for each group when experiment starts, within the most every 15 days, weigh once, laboratory observation is at the end of 45 days, all
Slaughter and cut open inspection, take 0.8g group matched group white mice liver, the heart, stomach, kidney do pathological examination.
2 result of the tests
2.1 the acute toxicity tests
Test result indicate that, when lactobacillus feed additive gavage of the present invention is by 150g/kg dosage, do not cause white mice yet
Dead.The no abnormality seen compared with matched group white mice such as each experimental group white mice spirit, appetite, activity.Little Bai is respectively organized after cuing open inspection
The internal organs of Mus no abnormality seen phenomenon compared with matched group, histopathologic examination's also no abnormality seen pathological changes, it was demonstrated that white mice
Maximum tolerated dose more than stomach 150g/kg to lactobacillus feed additive of the present invention, can be considered nontoxic level.
2.2 chronic toxicity test results
2.2.1 clinical observation gavages each test group white mice spirit of lactobacillus feed additive of the present invention, appetite, work
Dynamic, hair color, fecaluria were observed through 45 days, be showed no obvious abnormal response, but the weightening finish of white mice is obvious, with matched group phase ratio
Different notable (0.05 ＞ P ＞ 0.01).
2.2.2 after cuing open a viewing test observation 45 days, whole white mice being cutd open inspection, result is respectively organized white mice internal organs and is not all sent out
Existing pathological change.
2.2.3 0.8g dosage group and the liver of matched group white mice, kidney, stomach, the heart, spleen tissue are done disease by pathologic examination
Reason histological observation, result does not finds pathological change.
3 brief summaries
Experiment proves that the maximum tolerated dose of lactobacillus feed additive gavage white mice of the present invention, can at more than 150g/kg
It is considered as nontoxic level.Gavage each group of 0.2～0.8g/20g dosage every day, observed through 45 days, all healthy, find no poisoning or dead
Phenomenon of dying and weightening finish substantially, are cutd open inspection and histological observation are not shown any abnormalities change.Thus it is the most nontoxic to confirm that the present invention gavages,
Safe and reliable.
Embodiment 7
Experiment of preventive effects: use 10 age in days children pig 900, wherein 100 is only used as matched group isolation and supports, use common
Feedstuff；100 are only used as blank group, use normal diet；Another 700 test isolated rearings, are randomly divided into 7 groups, often organize 100
Only, embodiment 1～5 and lactobacillus feed additive+the normal diet of comparative example 1～2 preparation, lactobacillus feed additive is used
Addition is normal diet 0.8%.Feeding manner is free choice feeding, freely drinks water, and after 24h, takes test group and matched group
In young pig intramuscular injection inoculation dilution after isolated hog cholera venom in sick pig body, record 120h death condition, test knot
Fruit refers to table 1 below.
Therapeutic effect is tested: use 10 age in days children pig 800, separating in sick pig body after 800 eye dripping inoculation dilutions
The hog cholera venom gone out, feeding manner is free choice feeding and freely drinks water, and after infecting 12h, wherein 100 is only used as matched group, makes
Use normal diet；Another 700 is test group, is randomly divided into 7 groups, often group 100, uses embodiment 1～5 and comparative example 1～2 system
Standby lactobacillus feed additive+normal diet, the addition of lactobacillus feed additive is the 0.8% of normal diet, continuously
The death condition that record 120h respectively organizes, result of the test refers to table 1 below.
The result of table 1 each process group preventing and treating swine fever
As shown in Table 1, the lactobacillus feed additive of embodiment 1～5 preparation has good prophylactic-therapeutic effect to swine fever, protects
Protect rate and survival rate all more than 90%, and the protective rate of the lactobacillus feed additive of comparative example 1～2 preparation and survival rate exist
Less than 65%, the lactobacillus feed additive effect of the embodiment of the present invention is better than the lactic acid bacteria of comparative example embodiment 1～2 preparation and raises
Feed additives.Understanding, the present invention is to play a role under the synergism of each raw material.
Embodiment 8
In order to observe the present invention gaining effect to piglet, carry out tests below:
1. trial drug
The lactobacillus feed additive of the embodiment of the present invention 5.
2. experimental animal and packet
Taking certain pig farm to grow up miscellaneous ablactational baby pig 20, pig body weight is close, and being divided into two groups: one group is test group, totally 10
Pig (public 5, female 5), adds lactobacillus feed additive in feedstuff；Another group is matched group, totally 10 pigs (public affairs 5, mothers
5), do not offer medicine.
3. test method
Raising by two component hurdles after being weighed on an empty stomach by piglet, test group adds 3g dosage by this by every pig every day from this very day
Invention is uniformly admixed in feedstuff, and matched group is not offerd medicine, and two groups of forage volumes thrown something and fed every day are equal, and every day, feedstuff all allowed pig search for food
Complete.Whole pigs are weighed by off-test on an empty stomach after one and a half months.Gain weight examination effect according to test group and matched group,
During testing, observe the daily state of pig, body condition etc. simultaneously.
4. result
The present invention the results are shown in Table 2 to the weight increment test of piglet:
Table 2
From in table 2, test group pig average every pig weightening finish 10.76kg, increases weight than matched group pig (6.99kg) more
3.77kg, rate of body weight gain is 72.7%, improves 25.8 percentage points than the rate of body weight gain (46.9%) of matched group pig.Additionally, test group
Pig there are no any abnormal conditions.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of the spirit or essential attributes of the present invention, it is possible to realize the present invention in other specific forms.Therefore, no matter
From the point of view of which point, all should regard embodiment as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all by fall in the implication of equivalency and scope of claim
Change is included in the present invention.
Although moreover, it will be appreciated that this specification is been described by according to embodiment, but the most each embodiment only wraps
Containing an independent technical scheme, this narrating mode of description is only that for clarity sake those skilled in the art should
Description can also be formed those skilled in the art through appropriately combined as an entirety, the technical scheme in each embodiment
May be appreciated other embodiments.
Claims (6)
1. the lactobacillus feed additive preventing and treating swine fever, it is characterised in that be made up of according to the raw material of weight portion following: breast
Acid bacterium 1-5 part, wood root 8-16 part, Herba thalictri squarrosi 5-12 part, Cortex Glyptostrobi Pensilis 10-18 part, Folium Sapii 15-23 part, Fructus Crataegi 20-28
Part.
The lactobacillus feed additive of preventing and treating swine fever the most according to claim 1, it is characterised in that by following according to weight
The raw material composition of part: lactic acid bacteria 2-4 part, wood root 10-14 part, Herba thalictri squarrosi 7-10 part, Cortex Glyptostrobi Pensilis 12-16 part, Folium Sapii
17-21 part, Fructus Crataegi 22-26 part.
The lactobacillus feed additive of preventing and treating swine fever the most according to claim 1, it is characterised in that by following according to weight
The raw material composition of part: lactic acid bacteria 3 parts, wood root 12 parts, Herba thalictri squarrosi 8 parts, Cortex Glyptostrobi Pensilis 14 parts, Folium Sapii 19 parts, Fructus Crataegi 24
Part.
4. a preparation method for the lactobacillus feed additive of the preventing and treating swine fever as described in claim 1-3 is arbitrary, its feature
It is, comprises the steps of:
1) wood root, Cortex Glyptostrobi Pensilis are pulverized, crossed 100 mesh sieves, add the deionized water of the two quality 10 times, at a temperature of 70 DEG C
Heated and stirred processes 3h, then at 98-100 DEG C, heated and stirred processes 0.8-1h, filters to get filtrate；
2) Herba thalictri squarrosi, Folium Sapii, Fructus Crataegi are pulverized, cross 150 mesh sieves, add the vinegar of three's mass 0.8 times, at 65-68
DEG C lower seal soaks 3h, then stir process 60min at a temperature of 120 DEG C, prepares mixed material；
3), after filtrate, mixed material being down to room temperature, mix with lactic acid bacteria and be incorporated in fermentation 6h at a temperature of 35 DEG C, then at 50 DEG C
At a temperature of stirring i.e. obtain lactobacillus feed additive to doing.
5. the application in pig feed of the lactobacillus feed additive as described in claim 1-3 is arbitrary.
6. the application in preventing and treating swine fever medicine of the lactobacillus feed additive as described in claim 1-3 is arbitrary.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610671903.1A CN106260642A (en) | 2016-08-15 | 2016-08-15 | A kind of lactobacillus feed additive preventing and treating swine fever and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610671903.1A CN106260642A (en) | 2016-08-15 | 2016-08-15 | A kind of lactobacillus feed additive preventing and treating swine fever and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106260642A true CN106260642A (en) | 2017-01-04 |
Family
ID=57671239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610671903.1A Pending CN106260642A (en) | 2016-08-15 | 2016-08-15 | A kind of lactobacillus feed additive preventing and treating swine fever and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106260642A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627796A (en) * | 2009-08-13 | 2010-01-20 | 宜春强微生物科技有限公司 | Liquid feed additive and preparation method thereof |
| CN101869220A (en) * | 2010-06-07 | 2010-10-27 | 浙江省农业科学院 | Traditional herbal medicine feed addictive for preventing and controlling swine high fever syndrome |
| CN102008513A (en) * | 2009-09-04 | 2011-04-13 | 蒋盛军 | Method for controlling high swine fever by using nose atomization microecological preparation |
| CN102232998A (en) * | 2011-04-15 | 2011-11-09 | 北京康华远景科技有限公司 | Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof |
| CN102920946A (en) * | 2012-11-28 | 2013-02-13 | 蒋常德 | Preparation method of Chinese herbal medicinal active fermentation broth for preventing and treating porcine infectious diseases |
| CN103053863A (en) * | 2013-02-06 | 2013-04-24 | 韶关金苹果饲料有限公司 | Swine feed additive with effects of enhancing immunity and deodorizing |
| CN103549141A (en) * | 2013-04-25 | 2014-02-05 | 北京生泰尔生物科技有限公司 | Chinese herbal medicine composite probiotic feed additive for pigs and preparation method of additive |
| CN104087424A (en) * | 2014-08-04 | 2014-10-08 | 安阳市晶华油脂工程有限公司 | Method for one-step extraction and separation of essential oil, water solution and lipid of fresh peony flowers |
| CN105211651A (en) * | 2015-09-26 | 2016-01-06 | 梁佳灵 | Feed feed and the production method thereof of wild boar children pig |
-
2016
- 2016-08-15 CN CN201610671903.1A patent/CN106260642A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627796A (en) * | 2009-08-13 | 2010-01-20 | 宜春强微生物科技有限公司 | Liquid feed additive and preparation method thereof |
| CN102008513A (en) * | 2009-09-04 | 2011-04-13 | 蒋盛军 | Method for controlling high swine fever by using nose atomization microecological preparation |
| CN101869220A (en) * | 2010-06-07 | 2010-10-27 | 浙江省农业科学院 | Traditional herbal medicine feed addictive for preventing and controlling swine high fever syndrome |
| CN102232998A (en) * | 2011-04-15 | 2011-11-09 | 北京康华远景科技有限公司 | Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof |
| CN102920946A (en) * | 2012-11-28 | 2013-02-13 | 蒋常德 | Preparation method of Chinese herbal medicinal active fermentation broth for preventing and treating porcine infectious diseases |
| CN103053863A (en) * | 2013-02-06 | 2013-04-24 | 韶关金苹果饲料有限公司 | Swine feed additive with effects of enhancing immunity and deodorizing |
| CN103549141A (en) * | 2013-04-25 | 2014-02-05 | 北京生泰尔生物科技有限公司 | Chinese herbal medicine composite probiotic feed additive for pigs and preparation method of additive |
| CN104087424A (en) * | 2014-08-04 | 2014-10-08 | 安阳市晶华油脂工程有限公司 | Method for one-step extraction and separation of essential oil, water solution and lipid of fresh peony flowers |
| CN105211651A (en) * | 2015-09-26 | 2016-01-06 | 梁佳灵 | Feed feed and the production method thereof of wild boar children pig |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103689296A (en) | Feed for ewe at later pregnancy and lactation period and preparation method thereof | |
| CN102302599A (en) | Chinese medicinal composition for preventing and treating porcine diarrhea | |
| CN109364123A (en) | A kind of fermented tcm and preparation method thereof improving performance of poultry | |
| CN104783003A (en) | Chinese herbal medicine complete feed for piglets and preparation method of Chinese herbal medicine complete feed | |
| WO2018188525A1 (en) | Composition for use in improving circulation in body and delaying aging as well as use thereof | |
| CN106729054A (en) | A kind of Chinese herbal feed additive for preventing and treating sheep endoparasite and preparation method thereof | |
| CN106578477A (en) | Special feed for cattle and preparation method of special feed | |
| CN105941917A (en) | Pig feed capable of preventing and treating dysentery and preparation method of pig feed | |
| CN105146228A (en) | Chinese herb compound preparation used for sow miscarriage prevention and preparing method thereof | |
| CN103598437B (en) | For improving the feed of Layer Production Performance | |
| CN104161205B (en) | A kind of sow feed additive and application thereof preventing yellow and white dysentery of piglet | |
| CN102743657B (en) | Traditional Chinese medicine composition for preventing piglet dysentery and preparation method thereof | |
| CN106858055A (en) | A kind of fermented feed for strengthening pigling immunity and preparation method thereof | |
| CN106173450A (en) | Improve compound feed additive and the feedstuff of pigeon immunity | |
| CN110664896B (en) | Anti-stress sudden death prevention compound fermentation traditional Chinese medicine additive and preparation method and application thereof | |
| CN109999100B (en) | Pharmaceutical composition for improving egg yield of free-range laying hens | |
| CN106900981A (en) | It is a kind of to be applied to the immunopotentiating composition containing micro-powder of Chinese herbs, xylo-oligosaccharide, enzyme preparation and probiotics in livestock and poultry cultivation | |
| CN108567875A (en) | A kind of Chinese medicine composition and its Chinese medicine preparation for treating salpingitis of layer chicken | |
| CN104547770A (en) | Fermented traditional Chinese medicament for promoting growth of livestock and poultry and preparation method for fermented traditional Chinese medicament | |
| CN114903956A (en) | Chinese herbal medicine composition for preventing and treating pig diseases and preparation method and application thereof | |
| CN106260642A (en) | A kind of lactobacillus feed additive preventing and treating swine fever and its preparation method and application | |
| CN105999037A (en) | Mongolian medicine composition for treating cow mange sickness | |
| CN106071252A (en) | Clear feed additive of swine fever poison and its preparation method and application | |
| CN106109908A (en) | A kind of mongolian medicine compositions treating sheep babesiasis | |
| CN104606627B (en) | A kind of pharmaceutical composition for treating grice diarrhoea and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20170414 Address after: Financial Building No. 28 Hebi Henan province 458030 City Qibin District Qishui Avenue 6 floor No. 1 Applicant after: Hebi Huifeng Biotechnology Co., Ltd. Address before: Hebi City, Henan province 458099 City Shancheng District Road West five alley No. 1 No. 30 hospital Applicant before: Wang Dongping |
|
| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170104 |
|
| RJ01 | Rejection of invention patent application after publication |