CN106243176A - The copper complex formazan preparation method of sucrose - Google Patents
The copper complex formazan preparation method of sucrose Download PDFInfo
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- CN106243176A CN106243176A CN201610613958.7A CN201610613958A CN106243176A CN 106243176 A CN106243176 A CN 106243176A CN 201610613958 A CN201610613958 A CN 201610613958A CN 106243176 A CN106243176 A CN 106243176A
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- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H23/00—Compounds containing boron, silicon, or a metal, e.g. chelates, vitamin B12
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
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Abstract
The invention discloses a kind of copper complex formazan preparation method of sucrose, it is by sucrose and mantoquita complex reaction, obtain sucrose copper solution, filter the most after cooling, obtaining filtering residue and filtrate, filtrate adds ethanol water and is settled out complex, by pelleting centrifugation sucking filtration, vacuum drying is dried, and obtains purer sucrose complex copper product；The copper ion concentration of detection filtering residue, is 1:4 according to the mol ratio of sucrose Yu copper ion, continuously adds sucrose and mantoquita, and the waste water after centrifugal sucking filtration returns to the mother solution as dissolving saccharose in reactor, and circular response continues to generate sucrose copper.The present invention prepares the process of sucrose copper, does not waste resource, reduces production cost, improves production efficiency.Obtain sucrose copper and there is safe efficient, the advantage of high performance-price ratio, can be applicable to the animal feed being suitable for producing comprehensive nutrition.
Description
Technical field
The present invention relates to a kind of complex, particularly sucrose copper complex and preparation method thereof, and disclose its purposes.
Background technology
Nineteen twenty-eight, the Hart of University of Wisconsin (WisconsinUniversity) occurs after feeding milk by research
The rat of anemia, proposes to add after copper and ferrum to the formation of haemachrome is this necessary viewpoint, important on threpsology of copper
Property just gains recognition.Hereafter research confirms that copper has close relationship with the hematopoiesis of body in succession.Animal goes out after lacking copper
The lowest pigment small cell anemia.Further study showed that, copper is by affecting the absorption of ferrum, discharging, transport and utilize and join
With hematopoiesis.Appropriate copper can promote the absorption of intestinal ferrum, promotes that internal ferrum is entered bone marrow by storing place, makes nothing
Machine ferrum becomes Organic Iron, completes the exchange between ferric iron and ferrous iron, thus the beneficially transport of ferrum and utilization.Copper can also add
Speed hemoglobin and the synthesis of porphyrin, promote erythroneocytosis ripe and discharge.
First animal is that blood copper reduces after lacking copper, and the decline of liver copper deposit then develops into serum and erythrocyte copper content subtracts
Few, form Hypocupremia (hypocupperemia).Suffering from the animal of Hypocupremia, the absorption of intestinal ferrum reduces, liver,kidney,spleen
And serum levels of iron reduces, serum average ferrum saturation reduces, and total ferrum integration capability improves, and in tissue, the ferrum of storage can not be mobilized.
Thus ferrum is gathered in various intracellular can not discharge and transport, bone marrow utilizes the process of ferrum to be suppressed, the synthesis of hemoglobin
Reducing, the period of maturation of erythroneocytosis extends, the lost of life of mature erythrocyte, iron plasma clearance time lengthening.Thus lead
Cause red cell volume reduces, and low pigment small cell anemia occurs.Now, it is administered orally or injects ferrum and vitamin B12The most invalid use,
And after giving a small amount of mantoquita, the metabolism disorder of ferrum can obtain and significantly improve.If intravenous injection mantoquita albumen, the disappearance of above-mentioned symptom
The rapidest.Accordingly, the impact of body iron metabolism and hematopoietic function is mainly carried out by copper by Ceruloplasmin.
Copper is the composition of many enzymes in body.Such as cytochrome C, tryrosinase, ascorbic acid oxidase, uricase
Copper is all contained with galactase.These enzymes are transformed into O at superoxide anion free radical2And H2O2During play important
Catalytic action.Additionally copper is also the important composition of superoxide dismutase and monoamine oxidase, MAO system.These enzymes are primarily present
In internal Elastic tissue and connective tissue, in catalyzed elastomeric protein peptide chain, lysine residue is changed into aldehyde radical.And by with
The similar aldehyde radical of another peptide chain intermolecular or amino carry out aldol condensation or aldimine condensation, and then are formed between elastin fiber
Covalent cross-linking structure.Elastic fiber is made to become insoluble state, to maintain toughness and the elasticity of tissue.After lacking copper, cell color
Element oxidase, catalase, phospholipid enzyme, the activity reduction of succinate dehydrogenase, the Phosphorylation events of fat delays, cell
Respiratory metabolism and fission process can not normally carry out.
Owing to after scarce copper, lysyl oxidase activity reduces, in elastin laminin and collagen fiber, covalent cross-linking structure forms barrier
Hindering, collagen and elastin laminin are ripe slowly, and tissue elastase content reduces, and the fragility causing blood vessel, skeleton etc. to be organized increases
Add, angiorrhexis, the osteodystrophy symptom such as skeleton deformity, fracture occur.Copper is as superoxide dismutase and cytochrome
Oxidasic prothetic group, can promote the synthesis of cephalin and participate in Energy Metabolism of Brain Tissue.Scarce copper can cause cephalin dyssynthesis,
Ataxia, hindlimb paralysis occur.In addition, cardiac muscle inner cell chromo-oxidase is the most sensitive to scarce copper, and scarce copper can cause cardiac muscle
Degeneration and heart failure.
Animal hair growth and Pigmented impact are confirmed by copper by many scholars.The physical characteristic of Pilus Caprae seu Ovis, including
Crimpiness is in the interior existence all relying on disulfide group, and disulfide group not only affects the arrangement of keratin long-chain in wool fiber and determines
To, also by the cross coupled of disulfide group or be combined with keratin simultaneously, form distinctive curling in wool fiber.And copper is at this
During be chiefly to facilitate the formation of disulfide group and the cross coupled of disulfide group or with keratic combination.Lack the sheep that copper is individual
Hair lacks crimpiness, and even straight line Pilus Caprae seu Ovis or " silk thread hair ", greatly have impact on the quality of Pilus Caprae seu Ovis.Sheep in default of curling
Wool elasticity is abnormal, and tensile strength reduces.In terms of the pigmentation of hair, copper can promote the formation of tryrosinase and strengthen
Its activity.And tryrosinase is the catalyst that tyrosine is changed into melanic premise DOPA.And by DOPA again
Be changed into melanic during be also required for the participation of copper.Therefore, some studies discovery, and in dark hair, copper content is relatively
High.Copper can make being faded by hair of animal after lacking, the most circumocular hair fugitive color.Coloured by the animal of hair in except pig
Outward, being faded by hair after lacking copper comes soon than exsanguine performance.It is believed that, the achromia of hair can be used for animal and lacks copper
Early diagnosis.Additionally, it is reported, the curling of Pilus Caprae seu Ovis disappears also can be as the early diagnosis of its scarce copper.
Copper is the prothetic group of brain superoxide dismutase, can promote the synthesis of cephalin, is again the auxiliary of cytochrome oxidase
Base, has the effect of transmission electronics to ensure the generation of ATP.Scarce copper will result in shrinking of brain tissues, grey matter and white matter generation regression
Sexually transmitted disease (STD) becomes, and neuron reduces, drowsiness, and the sensitivity stimulating body reduces.Copper the most also can cause cerebral tissue and neurocyte
There is pathological changes, cause ataxia and change of spirit.Copper has an effect on the function of organism endocrine body of gland, thus extensively regulates body
Various activities, copper can promote hypophysis growth hormone releasing (GH), thyrotropin (TSH), metakentrin (LH) and
Thyroliberin (ACTH), also can affect the synthesis of adrenocortical steroid and catecholamine.Otherwise, above-mentioned hormone
Change also can affect the metabolism of copper in body.In addition, copper can strengthen its effect, in swine rearing after being combined with some drugs
During, use high-copper coordinate antibiotics growth-promoting effect become apparent from than independent copper or antibiotics, its mechanism need into
One step research.
Many researchs in recent years all prove, there is also extremely close relation between copper and the reproduction of animal.Someone
Thinking, breeding activity is than maintaining the normal vital movement more copper of needs (Van Niekrk, 1988).When copper amount is normal, can
To maintain the During Pregnancy of animal to be normally carried out, after lacking copper, often there is embryonic death and absorbed, causing breeding potential to decline,
Lack the cattle fertility reduction that copper weed is herded on the ground, occur that post-estrus prolongs or suppresses simultaneously, though pregnancy sometimes, it is also possible to go out
Now miscarry.Hen is after raising with the serious feedstuff lacking copper (0.7-0.9 μ g/g) 20 weeks, and egg production reduces, copper in blood plasma, liver and egg
Level is less than normal, and the incubation rate of hatching egg declines rapidly, embryo's performance anemia of this hatching egg, and hypoevolutism is hatching 72-96h
Rear Bleeding rate is the highest.By inference, all these performances, it may be that due at fetal development in early days, erythrocytic generation and connective
The reason that tissue is obstructed.On the other hand, the inhibitory action of reproduction is also apparent from by copper, and its mechanism of action mainly has: 1.
Low concentration copper ion has certain toxic action to sperm；2. copper oxide can reduce the viscosity of cervical discharge, and energy
Dissolve cervical mucus, thus disturb the movement of sperm and the implantation of zygote；3. secretory endometrium cell neutral and alkali phosphoric acid
The activity of enzyme, carbonic anhydrase and β hyaluronidase is suppressed by copper ion.But copper ion has raising proliferative phase of endometrium cell
The effect of middle acid phosphatase, thus affect reproductive process；4. a part of copper ion that endometrium absorbs can disturb or a word used in person's names
The content of zinc, manganese, molybdenum, ferrum etc. and metabolism in anti-endo cell, and zinc the importance of fertility study the most in many ways obtain abundant
Certainly.
Copper is the trace element that animal is required, and they play an important role in animal body, lacks trace element permissible
Cause being greatly lowered of growth of animal and production performance, directly influence the culture benefit of raiser.Copper is in the conjunction of haemachrome
Become and erythrocytic maturation process plays an important role, being many kind enzyme such as Ferroxidase, lysyloxidase, peroxide
The constituent of dismutase and cytochrome C oxidase etc., in vivo pigmentation, neurotransmission and saccharide and protein,
Amino acid metabolism aspect plays an important role, and the most also participates in the composition of skeleton, by the calmness of hair color element and brain cell and spinal cord
Matter etc..
In feedstuff there is larger difference in content and the titer of contained trace element, needs by feed additive form
It is added.Producing and be used mostly the inorganic microelements such as copper sulfate in practice at present, inorganic microelement is in use
There is following defect: bioavailability is low, animal is only capable of utilizing the inorganic microelement of addition less than 10%, is not utilized
Part be then excluded external, not only caused waste but also increased animal body and the burden of environment；The additionally trace element of ionic state
Metal ion is affected by phytic acid, oxalic acid and cellulose in intestinal, reduces its absorbance further；Short of money between element simultaneously
Anti-effect also affects biological value, and vitamin can be damaged by the redox reaction between metal ion, so that nutrient
Mass flow is lost, and reduces its absorption rate.
Along with development and the progress of aquaculture of feed industry, the feed additive such as trace element, aminoacid has become joins
Close the indispensable raw material of feedstuff.People, according to different animals nutritional need, add the trace of various dose in mixed feed
The feed additive such as element, aminoacid carrys out balanced nutrients composition, and to reach to promote animal productiong, improve product quality, raising is raised
Material conversion ratio, reduces feeding cost, the purpose increased economic efficiency.
But, with amino-acid complex (chelating) trace element of the synthesis such as the higher lysine of price, methionine, glycine
Although biological value is higher, but its use cost is the most of a relatively high, especially under current feed market environment with keen competition, and fall
The demand of low use cost highlights day by day.
Therefore, in order to reduce use cost, the present inventor makes full use of the locality that Guangxi cane planting is wide, sugar industry is flourishing
Affluent resources, with sucrose as primary raw material, by the complex reaction of sucrose Yu mantoquita, obtain sucrose copper, greatly reduce enterprise
Production cost, improve the competitiveness of enterprise.
Summary of the invention
It is an object of the invention to for deficiencies of the prior art, it is provided that one has sexual valence safe efficient, high
The advantage of ratio, develops the even animal husbandry of organic trace element industry and will play the sucrose copper of positive guiding and impetus
Complex.
The present invention is achieved in that
The copper complex formazan preparation method of sucrose of the present invention comprises the steps:
Step one, by sucrose and water according to the ratio mix homogeneously of weight fraction 25-32:40-50, according to sucrose and mantoquita
Weight fraction is that 25-32:40-50 adds mantoquita, adds sodium hydrate aqueous solution regulation pH 11-12 and obtains reactant liquor；
Step 2, by step one gained reactant liquor temperature 60-75 DEG C, be stirred continuously under the conditions of be incubated 1-2 hour, cold filtration
Separate, obtain filtering residue and filtrate；
Step 3, take step 2 gained filtrate, be to add 75% ethanol water at 1: 2 by the weight ratio of filtrate and the ethanol water of 75%
Solution room temperature precipitates 1.5-3 hour, and precipitate is through centrifugal sucking filtration, the waste water obtained and precipitate, and precipitate is vacuum dried,
Detection i.e. obtains sucrose copper products；
Step 4, take in step 2 filtering residue and detect copper ion concentration therein, being 1:4 according to the mol ratio of sucrose Yu copper ion
In filtering residue, add sucrose and mantoquita, take the waste water after step 3 is centrifuged sucking filtration and dissolve addition sucrose and the filtering residue of mantoquita, hydrogenation
The pH 11-12 of aqueous solution of sodium oxide regulation solution obtains reactant liquor；
Step 5, taking in step 4 reactant liquor and repeat step 2, step 3 and step 4 and react, circular response generates sucrose
Copper.
Described mantoquita is any one in copper chloride or copper sulfate.
The solubility of described sodium hydrate aqueous solution is 0.1-2mol/L.
Described sucrose copper complex is as feed additive, and for feeding of pig, chicken, duck, cattle and sheep, addition is
200g/ ton feedstuff.
A pair lone pair electrons, simultaneously transition metal ions (M=Cu is had on the glycosidic bond of sucrose (few disaccharide)2+、Zn2+、Mn2+、
Fe2+、Ca2+) there is suitable unoccupied orbital, can be formed " coordination effect " with sucrose under suitable temperature environment, after chemical reaction network
Close formation complex state metal, beneficially animal body to absorb.It is as follows that it forms molecular structural formula:
Above-mentioned formula is sucrose trace element molecular structural formula, wherein M=Cu2+、Zn2+、Mn2+、Fe2+、Ca2+。
Because the reaction of sucrose trace-element complex is complicated, product is different from general amino-acid chelate, belongs to more
In macromolecular structure, the molecular weight not determined.
The above-mentioned mechanism preparing sucrose copper is by sucrose and Cu2+After reacting forming complex, utilize sucrose copper complex
Insoluble in ethanol, then Precipitation is dried and i.e. can get solid sucrose complex copper.
Inventor refilters once after first sucrose copper solution being cooled down before adding ethanol, its object is to excess
Trace element remains with filtering residue form, as one of sucrose circulation complex reaction thing, by filtrate: the weight of 75% ethanol solution
Amount carries out room temperature precipitation than=1: 2 addition 75% ethanol solution, and its principle is: the trace element of excess is insoluble in ethanol, therefore wants
First filtered this out before adding ethanol.The polarity of ethanol is more weak again, and sucrose copper this be polyhydroxy salt, polarity is relatively big,
According to the principle of similar intermiscibility, sucrose copper is slightly soluble in ethanol, is also insoluble in ethanol and sucrose followed by sucrose copper complex
It is dissolved in the feature of ethanol, therefore both are separated, it is possible to obtain purer sucrose copper products.And inventor's Application No.
201410463827.6, the patent of entitled " trace element sucrose complex feed additive and its preparation method and application " is
With cane molasses as primary raw material, adding various trace element and carry out complex reaction, obtain is that trace element sucrose complex is raised
Feed additives, is complicated premix material.Cane molasses own contain substantial amounts of impurity, have added again trace element complexation,
Trace element and the impurity of excess are not removed, and the product obtained contains impurity and excess trace element actually
Premix material, compared with the present invention, both action functions are the most different.
The copper complex formazan using method of sucrose of the present invention is: after mixing homogeneously with feedstuff, for pig, chicken, duck, cattle and sheep
Feeding, addition is 200g/ ton feedstuff.
Compared with prior art, the positive effect of the present invention is:
1, sucrose copper complex of the present invention, by the complex reaction of sucrose Yu mantoquita, obtains sucrose copper solution, add ethanol it
Front by sucrose copper solution cool down after refilter once, obtain filtering residue and filtrate, by filtrate: weight ratio=1 of 75% ethanol water:
After 2 addition 75% ethanol waters carry out room temperature precipitation, by pelleting centrifugation sucking filtration, vacuum drying is dried, is sieved, detects, obtain relatively
Pure sucrose copper products；The copper ion concentration of detecting step two filtering residue, is 1:4 according to the mol ratio of sucrose Yu copper ion, continues
Continuous add sucrose and mantoquita, step 3 is centrifuged the waste water after sucking filtration and returns in reactor the mother solution as dissolving saccharose, with
Identical reaction condition is circulated reaction to be continued to generate sucrose copper.The present invention prepares the process of sucrose copper, is that a circulation is anti-
The chemical process answered, it is achieved that the recycling of the energy, meets the requirement of Green Chemistry, reduces production cost, improve life
Produce efficiency.
2, sucrose copper complex of the present invention, has fine good palatability due to the addition of sucrose, and slight sugar sweet fragrance makes
Obtain poultry to like taking food.
3, the present invention is with sucrose as primary raw material, the sucrose copper obtained, with simple amino-acid complex (chelating)
The cost that trace element is used is compared, and present invention reduces the use cost of 20-30%.
4, the complex of sucrose copper of the present invention, dissolubility absorption good, easy, good stability, as feed additive, can be substantially
Reduce the excrement excretion of feedstuff source trace element, reduce the aquaculture pollution to environment, moreover it is possible to improve meat yellowish pink, enhancing body
Anti-oxidation function, significantly improve the vigor of GSH-Px in liver, muscle, improve serum T-AOC and T-SOD, reduce UN, aobvious
Work improves the Nutrient Apparent Digestibilities such as feedstuff CP, EE, calcium, phosphorus, increases the deposition of nitrogen, moreover it is possible to significantly improve serum levels of iron regulating liver-QI ferrum and contain
Amount.After sucrose manganese enters in animal stomach, generate absorbable organic copper with gastric acid slow reaction, so contribute to improving
The palatability of pig, promotes the feed intake of ablactational baby pig, promotes to absorb, reduce excretion, and can improve the immune disease-resistance of pig
Ability.
5, the complex of sucrose copper of the present invention, organic trace element amount ratio inorganic ions is few, and absorption rate is high
Going out 3-4 times, do not result in waste, problem of environmental pollution is improved simultaneously, and the safety to people is improved.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention,
And can be practiced according to the content of description, and in order to allow the above and other objects, features and advantages of the present invention can
Become apparent, below in conjunction with preferred embodiment, describe in detail as follows.
Detailed description of the invention
The following detailed description of the detailed description of the invention of the present invention, it, as the part of this specification, is come by embodiment
The principle of the present invention is described, other aspects of the present invention, feature and advantage thereof will be become apparent from by this detailed description.
Embodiment 1
The copper complex formazan preparation method of sucrose comprises the steps:
Step one, toward reactor adds the sucrose of 25 weight portions, after adding the water dissolution sucrose of 40 weight portions, hydro-oxidation sodium is water-soluble
Basified, the pH 11 of regulation solution；
Step 2, reacting by heating still, keep temperature in the kettle at 65 DEG C, and adding 39 weight portion copper chlorides, to carry out complex reaction 1 little
Time, course of reaction is stirred continuously, and generates sucrose copper solution, and cold filtration separates, and obtains filtering residue and filtrate；
Step 3, by filtrate: weight ratio=1 of 75% ethanol water: 2 add 75% ethanol waters carries out room temperature precipitation, precipitation
Time is 1.5 hours, and precipitate utilizes through centrifugal sucking filtration, the waste water reclamation obtained, and precipitate is vacuum dried, detects and get final product
To sucrose copper products；
Step 4, the copper ion concentration of detecting step two filtering residue, be 1:4 according to the mol ratio of sucrose Yu copper ion, continuously add
25 parts by weight of sucrose and 10 weight portion copper chlorides (copper ion concentration 29 weight portion here), after sucking filtration centrifugal in step 3
Waste water returns to the mother solution in reactor as dissolving saccharose, and hydro-oxidation sodium water solution alkalizes, the pH 11 of regulation solution；
Step 5, reacting by heating still, keep temperature in the kettle to carry out complex reaction, the condition of course of reaction and step between 65 DEG C
Two, step 3 is identical, and circular response continues to generate sucrose copper.
Embodiment 2
The copper complex formazan preparation method of sucrose comprises the steps:
Step one, toward reactor adds the sucrose of 28 weight portions, after adding the water dissolution sucrose of 45 weight portions, hydro-oxidation sodium is water-soluble
Basified, the pH 11 of regulation solution；
Step 2, reacting by heating still, keep temperature in the kettle at 70 DEG C, add 50 parts sulfuric acid copper and carry out complex reaction 1.5
Hour, course of reaction is stirred continuously, and generates sucrose copper solution, and cold filtration separates, and obtains filtering residue and filtrate, by filtrate；
Step 3, by filtrate: weight ratio=1 of 75% ethanol water: 2 add 75% ethanol waters carries out room temperature precipitation, precipitation
Time is 2 hours, and precipitate utilizes through centrifugal sucking filtration, the waste water reclamation obtained, and precipitate is vacuum dried, and detection i.e. obtains
Sucrose copper products；
The copper ion concentration of filtering residue in step 4, detecting step two, is 1:4 according to the mol ratio of sucrose Yu copper ion, continues to add
Enter 28 parts by weight of sucrose and 13 parts sulfuric acid copper (copper ion concentration 37 weight portion here), after step 2 is centrifuged sucking filtration
Waste water returns to the mother solution in reactor as dissolving saccharose, and hydro-oxidation sodium water solution alkalizes, the pH 11 of regulation solution；
Step 5, reacting by heating still, keep temperature in the kettle to carry out complex reaction at 70 DEG C, the condition of course of reaction and step 2,
Step 3 is identical, and circular response continues to generate sucrose copper.
Embodiment 3
The copper complex formazan preparation method of sucrose comprises the steps:
Step one, toward reactor adds the sucrose of 32 weight portions, after adding the water dissolution sucrose of 50 weight portions, hydro-oxidation sodium is water-soluble
Basified, the pH 12 of regulation solution；
Step 2, reacting by heating still, keep temperature in the kettle at 75 DEG C, and adding 50 weight portion copper chlorides, to carry out complex reaction 2 little
Time, course of reaction is stirred continuously, and generates sucrose copper solution, and cold filtration separates, and obtains filtering residue and filtrate；
Step 3, by filtrate: weight ratio=1 of 75% ethanol water: 2 add 75% ethanol waters carries out room temperature precipitation, precipitation
Time is 3 hours, and precipitate utilizes through centrifugal sucking filtration, the waste water reclamation obtained, and precipitate is vacuum dried, and detection i.e. obtains
Sucrose copper products；
The copper ion concentration of filtering residue in step 4, detecting step two, is 1:4 according to the mol ratio of sucrose Yu copper ion, continues to add
Enter 32 parts by weight of sucrose and 17 weight portion copper chlorides (copper ion concentration meeting 33 weight portions here), after step 2 is centrifuged sucking filtration
Waste water return in reactor the mother solution as dissolving saccharose, hydro-oxidation sodium water solution alkalizes, the pH 12 of regulation solution；
Step 5, reacting by heating still, keep temperature in the kettle to carry out complex reaction at 75 DEG C, the condition of course of reaction and step 2,
Step 3 is identical, and circular response continues to generate sucrose copper.
Application implementation is tested
1, experimental animal and feeding and management
One Day-old Broiler Chickens 60 is only randomly divided into two groups, often organizes 30 pigs, male and female half and half, and it is matched group that selection adds one group of copper sulfate, choosing
Being experimental group with adding organic copper one group, addition is all 60mg/kg.Broiler basal diet is consistent, and chicken only carries out routine and exempts from
Epidemic disease, diet and drinking-water are free, and monitoring in 24 hours.
2, the source of test material
Inorganic copper sulfate is commercial diet grade bluestone, and sucrose is same is then to be ground by Nanjing Zeweier Feed Co., Ltd.
System.
3, assay method and index
During test, sooner or later carry out feedstuff every day and weigh, calculate the day's expenditure of each group of feedstuff, and the 12nd, 24,36,48,
60 day early morning weighed the body weight of every chicken, added up and calculate the situation of growth of body weight.
4, result of the test
Table 1: each group broiler weight situation of change
During by above table it can be seen that Chickens focuses on off-test, will be apparently higher than adding sulfur with one group of body weight change of sucrose copper
Acid copper group, significant difference.
Table two: the feedstuff-meat ratio change following table of broiler
The feedstuff-meat ratio ratio adding sucrose copper group in upper table adds the low of copper sulfate group, illustrates that organic copper is easier to be absorbed and used,
Biological value is good, and utilization rate is high.
Compared with prior art, the positive effect of the present invention is:
1, sucrose copper complex of the present invention, by the complex reaction of sucrose Yu mantoquita, obtains sucrose copper solution, add ethanol it
Front by sucrose copper solution cool down after refilter once, obtain filtering residue and filtrate, by filtrate: weight ratio=1 of 75% ethanol water:
After 2 addition 75% ethanol waters carry out room temperature precipitation, by pelleting centrifugation sucking filtration, vacuum drying is dried, is sieved, detects, obtain relatively
Pure sucrose copper products；The copper ion concentration of detecting step two filtering residue, is 1:4 according to the mol ratio of sucrose Yu copper ion, continues
Continuous add sucrose and mantoquita, step 3 is centrifuged the waste water after sucking filtration and returns in reactor the mother solution as dissolving saccharose, with
Identical reaction condition is circulated reaction to be continued to generate sucrose copper.The present invention prepares the process of sucrose copper, is that a circulation is anti-
The chemical process answered, it is achieved that the recycling of the energy, meets the requirement of Green Chemistry, reduces production cost, improve life
Produce efficiency.
2, sucrose copper complex of the present invention, has fine good palatability due to the addition of sucrose, and slight sugar sweet fragrance makes
Obtain poultry to like taking food.
3, the present invention is with sucrose as primary raw material, the sucrose copper obtained, with simple amino-acid complex (chelating)
The cost that trace element is used is compared, and present invention reduces the use cost of 20-30%.
4, the complex of sucrose copper of the present invention, dissolubility absorption good, easy, good stability, as feed additive, can be substantially
Reduce the excrement excretion of feedstuff source trace element, reduce the aquaculture pollution to environment, moreover it is possible to improve meat yellowish pink, enhancing body
Anti-oxidation function, significantly improve the vigor of GSH-Px in liver, muscle, improve serum T-AOC and T-SOD, reduce UN, aobvious
Work improves the Nutrient Apparent Digestibilities such as feedstuff CP, EE, calcium, phosphorus, increases the deposition of nitrogen, moreover it is possible to significantly improve serum levels of iron regulating liver-QI ferrum and contain
Amount.After sucrose manganese enters in animal stomach, generate absorbable organic copper with gastric acid slow reaction, so contribute to improving
The palatability of pig, promotes the feed intake of ablactational baby pig, promotes to absorb, reduce excretion, and can improve the immune disease-resistance of pig
Ability.
5, the complex of sucrose copper of the present invention, organic trace element amount ratio inorganic ions is few, and absorption rate is high
Going out 3-4 times, do not result in waste, problem of environmental pollution is improved simultaneously, and the safety to people is improved.
The above is the preferred embodiment of the present invention, certainly can not limit the right model of the present invention with this
Enclose, it is noted that for those skilled in the art, under the premise without departing from the principles of the invention, also may be used
To make some improvement and variation, these improve and variation is also considered as protection scope of the present invention.
Claims (4)
1. the copper complex formazan preparation method of sucrose, it is characterised in that comprise the steps:
Step one, by sucrose and water according to the ratio mix homogeneously of weight fraction 25-32:40-50, according to sucrose and mantoquita
Weight fraction is that 25-32:40-50 adds mantoquita, adds sodium hydrate aqueous solution regulation pH 11-12 and obtains reactant liquor；
Step 2, by step one gained reactant liquor temperature 60-75 DEG C, be stirred continuously under the conditions of be incubated 1-2 hour, cold filtration
Separate, obtain filtering residue and filtrate；
Step 3, take step 2 gained filtrate, be to add 75% ethanol water at 1: 2 by the weight ratio of filtrate and the ethanol water of 75%
Solution room temperature precipitates 1.5-3 hour, and precipitate is through centrifugal sucking filtration, the waste water obtained and precipitate, and precipitate is vacuum dried,
Detection i.e. obtains sucrose copper products；
Step 4, take in step 2 filtering residue and detect copper ion concentration therein, being 1:4 according to the mol ratio of sucrose Yu copper ion
In filtering residue, add sucrose and mantoquita, take the waste water after step 3 is centrifuged sucking filtration and dissolve addition sucrose and the filtering residue of mantoquita, hydrogenation
The pH 11-12 of aqueous solution of sodium oxide regulation solution obtains reactant liquor；
Step 5, taking in step 4 reactant liquor and repeat step 2, step 3 and step 4 and react, circular response generates sucrose
Copper.
2. the copper complex formazan preparation method of sucrose as claimed in claim 1, it is characterised in that: described mantoquita be copper chloride or
Any one in copper sulfate.
3. the copper complex formazan preparation method of sucrose as claimed in claim 1, it is characterised in that: described sodium hydrate aqueous solution
Solubility is 0.1-2mol/L.
4. the copper complex formazan preparation method of sucrose as claimed in claim 1, it is characterised in that: described sucrose copper complex conduct
Feed additive, for feeding of pig, chicken, duck, cattle and sheep, addition is 200g/ ton feedstuff.
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| CN111349126A (en) * | 2020-03-23 | 2020-06-30 | 湖南华诚生物资源股份有限公司 | Steviolbioside metal copper complex and preparation method thereof |
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