CN106243176B - The copper complex formazan preparation method of sucrose - Google Patents
The copper complex formazan preparation method of sucrose Download PDFInfo
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- CN106243176B CN106243176B CN201610613958.7A CN201610613958A CN106243176B CN 106243176 B CN106243176 B CN 106243176B CN 201610613958 A CN201610613958 A CN 201610613958A CN 106243176 B CN106243176 B CN 106243176B
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- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H23/00—Compounds containing boron, silicon, or a metal, e.g. chelates, vitamin B12
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
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Abstract
The invention discloses a kind of copper complex formazan preparation methods of sucrose, it is by sucrose and mantoquita complex reaction, obtain sucrose copper solution, then it filters after cooling, filter residue and filtrate are obtained, filtrate is added ethanol water and is settled out complex compound, and precipitating centrifugation is filtered, vacuum drying drying obtains purer sucrose complexing copper products；The copper ion concentration for detecting filter residue is 1:4 according to the molar ratio of sucrose and copper ion, continuously adds sucrose and mantoquita, will be centrifuged filtered waste water back to the mother liquor in reaction kettle as dissolving saccharose, circular response continues generation sucrose copper.The present invention prepares the process of sucrose copper, not waste of resource, reduces production cost, improves production efficiency.Sucrose copper is obtained with safe and efficient, high performance-price ratio advantage, can be applied to be suitble to the production comprehensive animal feed of nutrition.
Description
Technical field
The present invention relates to a kind of complex compounds, especially sucrose copper complex and preparation method thereof, and disclose its purposes.
Background technique
Nineteen twenty-eight, the Hart of University of Wisconsin (WisconsinUniversity) after research feeding milk by occurring
The rat of anaemia, after proposing that addition copper and iron are necessary this viewpoint to the formation of ferroheme, copper is important in nutrition
Property just gains recognition.Hereafter research confirms that the hematopoiesis of copper and body has close relationship in succession.Animal goes out after lacking copper
Existing low pigment small cell anemia.Further study showed that copper is joined by influencing absorption, release, transport and the utilization of iron
With hematopoiesis.Suitable copper can promote the absorption of enteron aisle iron, promote intracorporal iron to enter marrow by storing place, make nothing
Machine iron becomes Organic Iron, the exchange between ferric iron and ferrous iron is completed, to be conducive to the transport and utilization of iron.Copper can also add
The synthesis of fast hemoglobin and porphyrin promotes erythroneocytosis mature and discharges.
Animal is the reduction of blood copper first after lacking copper, and the decline of liver copper deposit then develops to serum and red blood cell copper content subtracts
It is few, it is formed Hypocupremia (hypocupperemia).The absorption of animal with Hypocupremia, enteron aisle iron is reduced, liver,kidney,spleen
And serum levels of iron reduces, serum be averaged the reduction of iron saturation degree, the raising of total iron integration capability, and the iron stored in tissue cannot be mobilized.
Thus iron be gathered in it is various cannot discharge and transport into the cell, marrow is suppressed using the process of iron, the synthesis of hemoglobin
It reduces, the maturity period of erythroneocytosis extends, and the lost of life of mature erythrocyte, the iron plasma clearance time extends.Thus it leads
It causes red cell volume to reduce, low pigment small cell anemia occurs.At this time, take orally or inject iron and vitamin B12Without effectiveness,
And after giving a small amount of mantoquitas, the metabolic disorder of iron, which can get, to be significantly improved.If being injected intravenously mantoquita albumen, the disappearance of above-mentioned symptom
It is more rapid.Accordingly, influence of the copper to body iron metabolism and hematopoietic function is mainly carried out by ceruloplasmin.
Copper is the composition of many enzymes in body.Such as cromoci, tyrosinase, ascorbic acid oxidase, uricase
Contain copper with galactase.These enzymes are transformed into O in superoxide anion free radical2And H2O2During play it is important
Catalytic action.In addition copper is also the important composition of superoxide dismutase and monoamine oxidase system.These enzymes are primarily present
In intracorporal elastic fibrous tissue and connective tissue, lysine residue is changed into aldehyde radical in catalyzed elastomeric protein peptide chain.And by with
The similar aldehyde radical or amino of intermolecular another peptide chain carry out aldol condensation or aldimine condensation, and then are formed between elastic protein fiber
Covalently cross-linked structure.Elastomer is set to become insoluble state, to maintain the toughness and elasticity of tissue.After lacking copper, cell color
The activity reduction of plain oxidizing ferment, catalase, phosphatide enzyme, succinate dehydrogenase, fatty Phosphorylation events delay, cell
Respiratory metabolism and fission process cannot normally carry out.
Since lysyl oxidase activity reduces after lacking copper, covalently cross-linked structure forms barrier in elastin laminin and collagenous fibres
Hinder, collagen and elastin laminin are mature slowly, and tissue elastase content is reduced, and the brittleness of the tissues such as blood vessel, bone is caused to increase
Add, angiorrhoxis, the osteodystrophies symptom such as skeleton deformity, fracture occurs.Copper is as superoxide dismutase and cytochromes
The prothetic group of oxidizing ferment can promote the synthesis of cephalin and participate in Energy Metabolism of Brain Tissue.Scarce copper can cause cephalin dyssynthesis,
There is incoordination, hindlimb paralysis.In addition, myocardium inner cell chromo-oxidase is more sensitive to scarce copper, scarce copper can cause cardiac muscle
Denaturation and heart failure.
Copper grows animal hair and pigmented influence has been confirmed by many scholars.The physical characteristic of wool, including
Crimpiness all relies on the presence of disulfide group inside, and disulfide group not only influences the arrangement of keratin long-chain in wool fiber and determines
To, while also being combined by the intersection of disulfide group or in conjunction with keratin, forming distinctive curling in wool fiber.And copper is herein
Be chiefly to facilitate in the process disulfide group formation and disulfide group intersection combine or and keratin combination.Lack the sheep of copper individual
Hair lacks crimpiness, even straight line wool or " silk thread hair ", greatly affected the quality of wool.In default of curling
Wool elasticity is abnormal, and tensile strength reduces.In terms of the pigmentation of hair, copper can promote the formation of tyrosinase and increase
Its strong activity.And tyrosinase is the premise that tyrosine is changed into melanin --- the catalyst of DOPA.And by DOPA
The participation of copper is also required for during being changed into melanin again.Therefore, some research discoveries, in dark hair copper content compared with
It is high.Copper can make the coat of animal fade after lacking, especially circumocular hair most fugitive color.Pig is removed in the animal of coloured coat
Outside, performance of the coat colour fading than anemia comes fastly after lacking copper.It is believed that the achroa of hair, which can be used for animal, lacks copper
Early diagnosis.In addition, it is reported that, the curling of wool, which disappears, also can be used as its early diagnosis for lacking copper.
Copper is the prothetic group of brain superoxide dismutase, can promote the synthesis of cephalin, and is the auxiliary of cytochrome oxidase
Base has the function of transmitting generation of the electronics to guarantee ATP.Scarce copper will cause shrinking of brain tissues, and regression occurs for grey matter and white matter
Venereal disease becomes, and neuron is reduced, drowsiness, reduces to the susceptibility of body stimulation.Copper excessively can also cause brain tissue and nerve cell
Lesion occurs, causes incoordination and spirit variation.Copper has an effect on the function of organism endocrine body of gland, to adjust body extensively
Various activities, copper can promote hypophysis growth hormone releasing (GH), thyrotropic hormone (TSH), metakentrin (LH) and
Corticotropin (ACTH), can also influence the synthesis of adrenocorticotro and catecholamine.Otherwise it is above-mentioned to swash
The variation of element also will affect the metabolism of copper in body.In addition, copper in conjunction with some drugs after can enhance its effect, in swine rearing
In the process, with high-copper cooperation antibiotic growth-promoting effect become apparent than individually with copper or antibiotic, mechanism need into
One step research.
Many researchs in recent years prove that there is also extremely close relationships between copper and the reproduction of animal.Someone
Think, breeding activity is than maintaining normal vital movement to need more copper (Van Niekrk, 1988).It, can when amount of copper is normal
To maintain the During Pregnancy of animal to be normally carried out, after lacking copper, embryonic death often occurs and is absorbed, breeding potential is caused to decline,
Lacking the ox fertility that copper weed is herded on the ground reduces, while post-estrus occurs and prolonging or inhibit, even if being pregnant sometimes, it is also possible to go out
Now miscarry.After raising seriously to lack feed 20 weeks of copper (0.7-0.9 μ g/g), egg production reduces hen, copper in blood plasma, liver and egg
Lower than normally, the hatching rate of hatching egg declines rapidly level, and the embryo of this hatching egg shows anaemia, and hypoevolutism is being incubated for 72-96h
Bleeding rate is very high afterwards.By inference, all these performances, it may be that due in embryonic development early stage, the generation and connective of red blood cell
The reason that tissue is obstructed.On the other hand, copper is also apparent from the inhibiting effect of reproduction, and mechanism of action mainly has: 1.
Low concentration copper ion has certain toxic action to sperm；2. copper oxide can reduce the viscosity of cervical discharge, and energy
Cervical mucus is dissolved, to interfere the movement of sperm and the implantation of zygote；3. secretory endometrium cell neutral and alkali phosphoric acid
The activity of enzyme, carbonic anhydrase and β hyaluronidase is inhibited by copper ion.But copper ion, which has, improves proliferative phase of endometrium cell
The effect of middle acid phosphatase, to influence reproductive process；4. a part of copper ion that endometrium absorbs can interfere or a word used in person's names
The content and metabolism of zinc, manganese, molybdenum, iron etc. in anti-endo cell, and research has obtained sufficiently importance of the zinc to fertility in many ways
Certainly.
Copper is microelement necessary to animal, they play an important role in animal body, and lacking microelement can be with
Cause being greatly lowered for growth of animal and production performance, directly influences the culture efficiency of raiser.Conjunction of the copper in ferroheme
It is many kinds of enzymes such as Ferroxidase, lysyloxidase, peroxide at playing an important role in the maturation with red blood cell
The constituent of mutase and cytochrome C oxidase etc., in vivo pigment deposition, neurotransmission and carbohydrate and protein,
It plays an important role in terms of amino acid metabolism, while also participating in composition, the calmness of coat pigment and the brain cell and spinal cord of bone
Matter etc..
The content and potency of contained microelement are needed there are larger difference through feed addictive form in feedstuff
It is added.It is mostly in use using inorganic microelements, inorganic microelements such as copper sulphate in production and practice at present
Have the following deficiencies: that bioavailability is low, animal is only capable of not being utilized using the inorganic microelement below of additive amount 10%
Part is then excluded the burden for not only causing to waste but also increase animal body and environment in vitro；In addition the microelement gold of ionic state
Belonging to ion is influenced in enteron aisle by phytic acid, oxalic acid and cellulose, its absorptivity is further decreased；Antagonism between element simultaneously
Effect also influences biological value, and the redox reaction between metal ion can damage vitamin, to make nutriment
It is lost, reduces its absorption rate.
With the development of feed industry and the progress of aquaculture, the feed addictives such as microelement, amino acid, which have become, matches
Close the indispensable raw material of feed.The micro of various dose is added according to different animals nutritional need in people in mixed feed
The feed addictives such as element, amino acid carry out balanced nutrients composition, promote animal productiong, improvement product quality to reach, improve and raise
Expect conversion ratio, reduces feeding cost, the purpose increased economic efficiency.
However, with amino-acid complex (chelating) microelement of the synthesis such as the higher lysine of price, methionine, glycine
Although biological value is higher, its use cost is also relatively high, especially under current feed market environment with keen competition, drop
The demand of low use cost is increasingly prominent.
Therefore, in order to reduce use cost, the present inventor makes full use of the locality that Guangxi cane planting is wide, sugar industry is flourishing
Affluent resources, by the complex reaction of sucrose and mantoquita, obtain sucrose copper, greatly reduce enterprise using sucrose as primary raw material
Production cost, improve the competitiveness of enterprise.
Summary of the invention
It is a kind of with safe and efficient, high sexual valence the purpose of the present invention is In view of the above shortcomings of the prior art, providing
The advantage of ratio will play the sucrose copper of positive guidance and impetus to the development of organic trace element industry even animal husbandry
Complex compound.
The present invention is implemented as follows:
The copper complex formazan preparation method of sucrose of the present invention includes the following steps:
Step 1: sucrose and water are uniformly mixed according to the ratio of weight fraction 25-32:40-50, according to sucrose and mantoquita
Weight fraction be 25-32:40-50 be added mantoquita, be added sodium hydrate aqueous solution adjust pH 11-12 obtain reaction solution；
Step 2: reaction solution obtained by step 1 is kept the temperature 1-2 hours under the conditions of 60-75 DEG C of temperature, being stirred continuously, it is cooling
It is separated by filtration, obtains filter residue and filtrate；
It is 1: 2 addition 75% by the weight ratio of filtrate and 75% ethanol water Step 3: taking filtrate obtained by step 2
Ethanol water room temperature precipitates 1.5-3 hours, and sediment is filtered by centrifugation, obtained waste water and sediment, and sediment is through true
Sky is dry, and detection obtains sucrose copper products；
Step 4: taking filter residue in step 2 and detecting copper ion concentration therein, according to the molar ratio of sucrose and copper ion
Sucrose and mantoquita are added into filter residue for 1:4, step 3 is taken to be centrifuged the filter residue that sucrose and mantoquita is added in filtered waste water dissolution,
The pH 11-12 that adding sodium hydroxide aqueous solution adjusts solution obtains reaction solution；
Step 5: reaction solution in step 4 is taken to repeat Step 2: step 3 and step 4 are reacted, circular response is generated
Sucrose copper.
The mantoquita is any one of copper chloride or copper sulphate.
The concentration of the sodium hydrate aqueous solution is 0.1-2mol/L.
The sucrose copper complex is as feed addictive, and for the feeding of pig, chicken, duck, ox and sheep, additive amount is
200g/ tons of feeds.
There are a pair of of lone pair electrons, while transition metal ions (M=Cu on the glycosidic bond of sucrose (few disaccharides)2+、Zn2+、 Mn2 +、Fe2+、Ca2+) there is suitable unoccupied orbital, under suitable temperature environment " coordination effect ", after chemical reaction can be formed with sucrose
Complexing forms complex state metal, is absorbed and utilized conducive to animal body.It is as follows that it forms molecular structural formula:
Above-mentioned formula is sucrose microelement molecular structural formula, wherein M=Cu2+、Zn2+、Mn2+、Fe2+、Ca2+。
Because the reaction of sucrose trace-element complex is complicated, product is different from general amino-acid chelate, belong to more
In macromolecular structure, the molecular weight that does not determine.
The above-mentioned mechanism for preparing sucrose copper is by sucrose and Cu2+After reacting forming complex, sucrose copper complex is utilized
Insoluble in ethyl alcohol, then solid sucrose complex copper is can be obtained in drying to Precipitation.
Inventor is first primary refiltering after the cooling of sucrose copper solution before ethyl alcohol is added, and its object is to excessive
Microelement is remained in the form of filter residue, as one of sucrose circulation complex reaction object, by filtrate: the weight of 75% ethanol solution
It measures ratio=1: 2 and 75% ethanol solution progress room temperature precipitating is added, principle is: excessive microelement does not dissolve in ethyl alcohol, therefore
It to be filtered this out first before ethyl alcohol is added.Again ethyl alcohol polarity it is weaker, and sucrose copper this be polyhydroxy salt, polarity compared with
Greatly, according to the principle of similar intermiscibility, sucrose copper is slightly soluble in ethyl alcohol, is also insoluble in ethyl alcohol and sugarcane followed by sucrose copper complex
Sugar is dissolved in the characteristics of ethyl alcohol, therefore the two is separated, so that it may obtain purer sucrose copper products.And inventor application No. is
201410463827.6 the patent of entitled " microelement sucrose complex compound feed addictive and its preparation method and application " is
Using cane molasses as primary raw material, various microelements are added and carry out complex reaction, what is obtained is that microelement sucrose complex compound is raised
Feed additives are complicated premix.Just contain a large amount of impurity in cane molasses itself, and microelement complexing be added,
Excessive microelement and impurity are not removed, and obtained product is containing impurity and excessive microelement actually
Premix, compared with the present invention, the action function of the two is completely different.
The copper complex formazan application method of sucrose of the present invention is: after mixing with feed, for pig, chicken, duck, ox and sheep
Feeding, additive amount are 200g/ tons of feeds.
Compared with prior art, the positive effect of the present invention is:
1, sucrose copper complex of the present invention obtains sucrose copper solution by the complex reaction of sucrose and mantoquita, and second is being added
It will be refiltered once after the cooling of sucrose copper solution before alcohol, and filter residue and filtrate be obtained, by filtrate: the weight of 75% ethanol water
After 75% ethanol water progress room temperature precipitating is added than=1: 2, precipitating centrifugation is filtered, vacuum drying is dried, screening, inspection
It surveys, obtains purer sucrose copper products；The copper ion concentration of two filter residue of detecting step, according to the molar ratio of sucrose and copper ion
For 1:4, sucrose and mantoquita are continuously added, step 3 is centrifuged filtered waste water and is returned in reaction kettle as dissolving saccharose
Mother liquor, with identical reaction condition carry out circular response continue generate sucrose copper.The present invention prepares the process of sucrose copper, is one
The chemical process of a circular response realizes the recycling of the energy, meets the requirement of Green Chemistry, reduces production cost,
Improve production efficiency.
2, sucrose copper complex of the present invention, due to sucrose addition and have fine palatability good, slight sugar sweet fragrance makes
It obtains livestock and poultry and likes feeding.
3, the present invention is using sucrose as primary raw material, obtained sucrose copper, uses amino-acid complex (chelating) with simple
Cost used in microelement is compared, and present invention reduces the use costs of 20-30%.
4, the complex compound of sucrose copper of the present invention, dissolubility is good, it is easy absorb, stability it is good, can be obvious as feed addictive
The excrement excretion of feed source microelement is reduced, pollution of the aquaculture to environment is reduced, moreover it is possible to improve meat yellowish pink, enhance body
Anti-oxidation function, significantly improve the vigor of liver, GSH-Px in muscle, improve serum T-AOC and T-SOD, reduce UN, show
Writing improves the Nutrient Apparent Digestibilities such as feed CP, EE, calcium, phosphorus, increases the deposition of nitrogen, moreover it is possible to significantly improve serum levels of iron and liver iron contains
Amount.After sucrose manganese enters in animal stomach, absorbable organic copper is generated with gastric acid slow reaction, facilitates to improve in this way
The palatability of pig promotes the feed intake of weanling pig, promotes to absorb, reduces excretion, and the immune disease-resistance of pig can be improved
Ability.
5, the complex compound of sucrose copper of the present invention, organic trace element amount ratio inorganic ions is few, and absorption rate is high
It 3-4 times out, not will cause waste, while problem of environmental pollution is improved, the safety of people be improved.
The above description is only an overview of the technical scheme of the present invention, in order to better understand the technical means of the present invention,
And it can be implemented in accordance with the contents of the specification, and in order to allow above and other objects, features and advantages of the invention can
It is clearer and more comprehensible, below in conjunction with preferred embodiment, detailed description are as follows.
Specific embodiment
The following detailed description of a specific embodiment of the invention, as part of this specification, by embodiment come
Illustrate that the principle of the present invention, other aspects of the present invention, feature and its advantage will become apparent by the detailed description.
Embodiment 1
The copper complex formazan preparation method of sucrose includes the following steps:
Step 1: add the sucrose of 25 parts by weight into reaction kettle, and after the water dissolving saccharose for adding 40 parts by weight, adding sodium hydroxide
It is water-soluble basified, adjust the pH 11 of solution；
Step 2: heating reaction kettle, keeps temperature in the kettle at 65 DEG C, adds 39 parts by weight copper chlorides and carry out complex reaction
1 hour, reaction process was stirred continuously, and generated sucrose copper solution, and cold filtration separation obtains filter residue and filtrate；
Step 3: pressing filtrate: weight ratio=1 of 75% ethanol water: 2, which are added 75% ethanol water progress room temperature, sinks
It forms sediment, the sedimentation time is 1.5 hours, and sediment is filtered by centrifugation, and obtained Sewage treatment utilizes, and sediment is vacuum dried,
Detection obtains sucrose copper products；
Step 4: the copper ion concentration of two filter residue of detecting step, is 1:4 according to the molar ratio of sucrose and copper ion, continues
25 parts by weight of sucrose and 10 parts by weight copper chlorides (29 parts by weight of copper ion concentration here) are added, are filtered being centrifuged in step 3
Waste water afterwards returns to the mother liquor in reaction kettle as dissolving saccharose, and adding sodium hydroxide is water-soluble basified, adjusts the pH of solution
11；
Step 5: heating reaction kettle, keeps temperature in the kettle to carry out complex reaction between 65 DEG C, the condition of reaction process with
Step 2: step 3 is identical, circular response continues to generate sucrose copper.
Embodiment 2
The copper complex formazan preparation method of sucrose includes the following steps:
Step 1: add the sucrose of 28 parts by weight into reaction kettle, and after the water dissolving saccharose for adding 45 parts by weight, adding sodium hydroxide
It is water-soluble basified, adjust the pH 11 of solution；
Step 2: heating reaction kettle, keeps temperature in the kettle at 70 DEG C, adds 50 parts sulfuric acid copper and carry out complex reaction
1.5 hours, reaction process was stirred continuously, and generated sucrose copper solution, and cold filtration separation obtains filter residue and filtrate, by filtrate；
Step 3: pressing filtrate: weight ratio=1 of 75% ethanol water: 2, which are added 75% ethanol water progress room temperature, sinks
It forms sediment, the sedimentation time is 2 hours, and sediment is filtered by centrifugation, and obtained Sewage treatment utilizes, and sediment is vacuum dried, inspection
It surveys and obtains sucrose copper products；
Step 4: in detecting step two filter residue copper ion concentration, according to the molar ratio of sucrose and copper ion be 1:4, after
It is continuous that 28 parts by weight of sucrose and 13 parts sulfuric acid copper (37 parts by weight of copper ion concentration here) are added, step 2 is centrifuged and is taken out
Waste water after filter returns to the mother liquor in reaction kettle as dissolving saccharose, and adding sodium hydroxide is water-soluble basified, adjusts the pH of solution
11；
Step 5: heating reaction kettle, keeps temperature in the kettle in 70 DEG C of progress complex reactions, the condition and step of reaction process
Two, step 3 is identical, and circular response continues to generate sucrose copper.
Embodiment 3
The copper complex formazan preparation method of sucrose includes the following steps:
Step 1: add the sucrose of 32 parts by weight into reaction kettle, and after the water dissolving saccharose for adding 50 parts by weight, adding sodium hydroxide
It is water-soluble basified, adjust the pH 12 of solution；
Step 2: heating reaction kettle, keeps temperature in the kettle at 75 DEG C, adds 50 parts by weight copper chlorides and carry out complex reaction
2 hours, reaction process was stirred continuously, and generated sucrose copper solution, and cold filtration separation obtains filter residue and filtrate；
Step 3: pressing filtrate: weight ratio=1 of 75% ethanol water: 2, which are added 75% ethanol water progress room temperature, sinks
It forms sediment, the sedimentation time is 3 hours, and sediment is filtered by centrifugation, and obtained Sewage treatment utilizes, and sediment is vacuum dried, inspection
It surveys and obtains sucrose copper products；
Step 4: in detecting step two filter residue copper ion concentration, according to the molar ratio of sucrose and copper ion be 1:4, after
It is continuous that 32 parts by weight of sucrose and 17 parts by weight copper chlorides (33 parts by weight of copper ion concentration meeting here) are added, step 2 is centrifuged
Filtered waste water returns to the mother liquor in reaction kettle as dissolving saccharose, and adding sodium hydroxide is water-soluble basified, adjusts solution
pH 12；
Step 5: heating reaction kettle, keeps temperature in the kettle in 75 DEG C of progress complex reactions, the condition and step of reaction process
Two, step 3 is identical, and circular response continues to generate sucrose copper.
Application implementation test
1, experimental animal and feeding management
One Day-old Broiler Chickens 60 are only randomly divided into two groups, and every group of 30 pigs, half male and half female, is selected plus one group of copper sulphate is control
Group, selecting one group of organic copper of addition is experimental group, and additive amount is all 60mg/kg.Broiler chicken basal diet is consistent, and chicken only carries out often
Rule are immune, and diet and drinking-water are free, and 24 hours monitor.
2, the source of test material
Inorganic copper sulphate is commercial diet grade bluestone, and same sucrose is then by Nanjing Zeweier Feed Co., Ltd.
It develops.
3, measuring method and index
During test, carry out feed weighing sooner or later daily, calculate the day's expenditure of each group feed, and the 12nd, 24,
36,48,60 day early morning weighed the weight of every chicken, counted and calculate the situation of growth of weight.
4, test result
Table 1: each group broiler weight situation of change
| Project | Copper sulphate group (g) | Sucrose copper group (g) |
| 12nd day | 269±20.4 | 299±31.2 |
| 24th day | 801±64.2 | 918±102.2 |
| 36th day | 1621±211.4 | 1799±188.4 |
| 48th day | 2682±222.5 | 2924±312.5 |
| 60th day | 3148±206.7 | 3541±158.6 |
When Chickens focus on off-test it can be seen from the above table, one group of changes of weight of sucrose copper is added to be apparently higher than
Add copper sulphate group, significant difference.
Table two: the feedstuff-meat ratio of broiler chicken changes following table
| Project | Copper sulphate group | Sucrose copper group |
| 36th day | 1.689±0.11 | 1.468±0.1. |
| 60th day | 2.324±0.08 | 2.050±0.09 |
The feedstuff-meat ratio that sucrose copper group is added in upper table is lower than addition copper sulphate group, illustrates that organic copper is easier to be absorbed benefit
With biological value is good, and utilization rate is high.
Compared with prior art, the positive effect of the present invention is:
1, sucrose copper complex of the present invention obtains sucrose copper solution by the complex reaction of sucrose and mantoquita, and second is being added
It will be refiltered once after the cooling of sucrose copper solution before alcohol, and filter residue and filtrate be obtained, by filtrate: the weight of 75% ethanol water
After 75% ethanol water progress room temperature precipitating is added than=1: 2, precipitating centrifugation is filtered, vacuum drying is dried, screening, inspection
It surveys, obtains purer sucrose copper products；The copper ion concentration of two filter residue of detecting step, according to the molar ratio of sucrose and copper ion
For 1:4, sucrose and mantoquita are continuously added, step 3 is centrifuged filtered waste water and is returned in reaction kettle as dissolving saccharose
Mother liquor carries out circular response with identical reaction condition and continues to generate sucrose copper.The present invention prepares the process of sucrose copper, is one
The chemical process of circular response realizes the recycling of the energy, meets the requirement of Green Chemistry, reduces production cost, mentions
High production efficiency.
2, sucrose copper complex of the present invention, due to sucrose addition and have fine palatability good, slight sugar sweet fragrance makes
It obtains livestock and poultry and likes feeding.
3, the present invention is using sucrose as primary raw material, obtained sucrose copper, uses amino-acid complex (chelating) with simple
Cost used in microelement is compared, and present invention reduces the use costs of 20-30%.
4, the complex compound of sucrose copper of the present invention, dissolubility is good, it is easy absorb, stability it is good, can be obvious as feed addictive
The excrement excretion of feed source microelement is reduced, pollution of the aquaculture to environment is reduced, moreover it is possible to improve meat yellowish pink, enhance body
Anti-oxidation function, significantly improve the vigor of liver, GSH-Px in muscle, improve serum T-AOC and T-SOD, reduce UN, show
Writing improves the Nutrient Apparent Digestibilities such as feed CP, EE, calcium, phosphorus, increases the deposition of nitrogen, moreover it is possible to significantly improve serum levels of iron and liver iron contains
Amount.After sucrose manganese enters in animal stomach, absorbable organic copper is generated with gastric acid slow reaction, facilitates to improve in this way
The palatability of pig promotes the feed intake of weanling pig, promotes to absorb, reduces excretion, and the immune disease-resistance of pig can be improved
Ability.
5, the complex compound of sucrose copper of the present invention, organic trace element amount ratio inorganic ions is few, and absorption rate is high
It 3-4 times out, not will cause waste, while problem of environmental pollution is improved, the safety of people be improved.
The above is a preferred embodiment of the present invention, cannot limit the right model of the present invention with this certainly
It encloses, it is noted that for those skilled in the art, without departing from the principle of the present invention, may be used also
To make several improvement and variation, these, which improve and change, is also considered as protection scope of the present invention.
Claims (2)
1. a kind of copper complex formazan preparation method of sucrose, which comprises the steps of:
Step 1: sucrose and water are uniformly mixed according to the ratio of weight fraction 25-32:40-50, according to the weight of sucrose and mantoquita
Measuring score is that mantoquita is added in 25-32:40-50, and sodium hydrate aqueous solution adjusting pH 11-12 is added and obtains reaction solution；
Step 2: reaction solution obtained by step 1 is kept the temperature 1-2 hours under the conditions of 60-75 DEG C of temperature, being stirred continuously, cold filtration
Separation, obtains filter residue and filtrate；
It is 1: 2 75% ethyl alcohol of addition by the weight ratio of filtrate and 75% ethanol water Step 3: taking filtrate obtained by step 2
Aqueous solution room temperature precipitates 1.5-3 hours, and sediment is filtered by centrifugation, obtained waste water and sediment, and sediment is dry through vacuum
Dry, detection obtains sucrose copper products；
It is 1:4 according to the molar ratio of sucrose and copper ion Step 4: taking filter residue in step 2 and detecting copper ion concentration therein
Sucrose and mantoquita are added into filter residue, takes step 3 to be centrifuged the filter residue that sucrose and mantoquita is added in filtered waste water dissolution, adds hydrogen
The pH 11-12 that aqueous solution of sodium oxide adjusts solution obtains reaction solution；
Step 5: reaction solution in step 4 is taken to repeat Step 2: step 3 and step 4 are reacted, circular response generates sucrose
Copper；
The mantoquita is any one of copper chloride or copper sulphate；
The concentration of the sodium hydrate aqueous solution is 0.1-2mol/L.
2. the copper complex formazan preparation method of sucrose as described in claim 1, it is characterised in that: the sucrose copper complex conduct
Feed addictive, for the feeding of pig, chicken, duck, ox and sheep, additive amount is 200g/ tons of feeds.
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| CN107827945A (en) * | 2017-11-21 | 2018-03-23 | 天峨县宏昌农机专业合作社 | The preparation method and its usage of the complex compound of secondary complex copper |
| CN111349126B (en) * | 2020-03-23 | 2021-04-06 | 江西海富生物工程有限公司 | Steviolbioside metal copper complex and preparation method thereof |
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| EP1819720B1 (en) * | 2004-12-06 | 2013-05-15 | Emcure Pharmaceuticals Limited | A cost-effective process for preparation of manufacture of iron sucrose |
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| CN101671373A (en) * | 2009-10-10 | 2010-03-17 | 天津中敖生物科技有限公司 | Preparation method for iron sucrose bulk drug and injection thereof |
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