CN106234036A - A kind of method that Tea Germplasm preserves - Google Patents

A kind of method that Tea Germplasm preserves Download PDF

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Publication number
CN106234036A
CN106234036A CN201610597565.1A CN201610597565A CN106234036A CN 106234036 A CN106234036 A CN 106234036A CN 201610597565 A CN201610597565 A CN 201610597565A CN 106234036 A CN106234036 A CN 106234036A
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CN
China
Prior art keywords
stock
scion
grafting
preserves
tea
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Pending
Application number
CN201610597565.1A
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Chinese (zh)
Inventor
黎娜
张帆
杨慧
肖力争
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Hunan Longping Tea Industry Hi-Tech Co Ltd
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Hunan Longping Tea Industry Hi-Tech Co Ltd
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Application filed by Hunan Longping Tea Industry Hi-Tech Co Ltd filed Critical Hunan Longping Tea Industry Hi-Tech Co Ltd
Priority to CN201610597565.1A priority Critical patent/CN106234036A/en
Publication of CN106234036A publication Critical patent/CN106234036A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting

Abstract

The present invention relates to a kind of method that Tea Germplasm preserves, it includes that choose Camellia sinensis giving birth to semi-lignified branch then is stock, cuts off stock level, and carries out splitting cutting;The short fringe of clip edible tender branch bud head or bud one leaf is scion;The scion cambium layer of clip is alignd with stock cambium layer, is inserted perpendicularly into stock otch;Bundle at grafting with grafting bar, clean Cotton Gossypii pure water has been infiltrated, is wrapped in the periphery of grafting bar, then bagging;Each scion PVC plastic is hung label be marked;Then shading treatment is carried out;After scion rudiment, progressively release kraft paper bag and bagging.The present invention uses the semi-lignified branch of giving birth to then of Camellia sinensis in strong age to be stock, the edible tender branch of the tea tree breed that clip need to preserve is scion, cut-grafting method is used to carry out grafting, operation is simple, can carry out the whole year, survival rate is up to 95%, and one strong age Camellia sinensis can preserve 15 40 tea tree breeds, can effectively reduce space, soil and management cost that Resources of Tea Plant preserves.

Description

A kind of method that Tea Germplasm preserves
Technical field
The present invention relates to the method that Tea Germplasm preserves.
Background technology
The Collection and conservation of Tea Germplasm, is to preserve and utilize the multifarious important means of nature Resources of Tea Plant, is Abundant and substantial breeding work and the material base of biological study.The major way that Resources of Tea Plant preserves at present is kind of a plant protection Deposit, including Camellia sinensis transplanting, seminal propagation and asexual propagation.Old Camellia sinensis transplanting success is relatively low, need to expend bigger manpower, thing Power, easily catches cold, drought and the natural disaster such as disease pest threaten, it is difficult to reach the requirement of long-term conserving species matter, and destructible ecology ring Border;Camellia sinensis belongs to cross-pollinatd plant, and seminal propagation can not preserve the entire genetic information of female parent;Asexual propagation is by kind, time Impact notable, and wild resource asexual propagation survival rate is extremely low, it is difficult to guarantee that Tea Germplasm effectively preserves.
Summary of the invention
For above-mentioned technical problem, the present invention provides a kind of survival rate Tea Germplasm high, easy and simple to handle, efficient to protect The method deposited.
The present invention solves the technical scheme that above-mentioned technical problem used: a kind of method that Tea Germplasm preserves, It comprises the following steps:
(1) selection of stock: stock need to select to be planted in the warmhouse booth Camellia sinensis of more than 3 years;
(2) stock prepares: choosing Camellia sinensis and giving birth to the branch of semi-lignified then is stock, carries out splitting cutting in stock horizontal cross-section;
(3) scion prepares: several tea tree breed edible tender branch that clip need to preserve respectively, the bud head of clip branch or bud one leaf Short fringe be scion;
(4) grafting: scion is inserted perpendicularly into stock and splits the otch cut;
(5) grafting post processing: bundle stock with grafting bar, infiltrates clean Cotton Gossypii pure water, is wrapped in the periphery of grafting bar, Bagging again;
(6) labelling: each scion PVC plastic is hung label and is marked;
(7) shelter from heat or light: be enclosed within outside bagging with kraft paper bag after labelling, carry out shading treatment;
(8) managing after grafting: after scion rudiment, progressively releases kraft paper bag and bagging.
As preferably, when choosing stock, cut off, in stock by secateurs level at branch from bottom to top 10-20cm Between position vertically cut otch, notch depth is 1-1.5cm.
As preferably, a length of 1-2cm of clip scion, scion bottom respectively cuts a cutter, forms wedge shape, bevel is flat, Directly.
As preferably, cut when taking bud head, the long 0.6-0.8cm of wound.
As preferably, when cutting the short fringe taking bud one leaf, the long 1-1.5cm of wound, and leaf cuts off half.
As can be known from the above technical solutions, the present invention uses the semi-lignified branch of giving birth to then of Camellia sinensis in strong age to be stock, clip The edible tender branch of the tea tree breed that need to preserve is scion, uses cut-grafting method to carry out grafting, simple to operate, can carry out the whole year, survives Rate is up to 95%, and the Camellia sinensis in a strong age can preserve 15-40 tea tree breed, effectively reduces the sky that Resources of Tea Plant preserves Between, soil and management cost.
Detailed description of the invention
The present invention is described more detail below, and illustrative examples and explanation in this present invention are used for explaining the present invention, But it is not as a limitation of the invention.
A kind of method that Tea Germplasm preserves, it comprises the following steps:
(1) stock prepares: choosing Camellia sinensis and giving birth to the branch of semi-lignified then is stock, and stock need to select to be planted in warmhouse booth 3 Camellia sinensis more than year;Carry out splitting cutting in stock horizontal cross-section;Specifically cut by secateurs level at branch from bottom to top 10-20cm Disconnected, vertically cut otch in the centre position of stock, notch depth is 1-1.5cm.
(2) scion prepares: several tea tree breed edible tender branch that clip need to preserve respectively, the bud head of clip branch or a bud The short fringe of one leaf is scion;The a length of 1-2cm of clip scion, scion bottom respectively cuts a cutter, forms wedge shape, bevel is flat, Directly;Cut when taking bud head, the long 0.6-0.8cm of wound;When cutting the short fringe taking bud one leaf, the long 1-1.5cm of wound, and leaf cuts off one Half.
(3) grafting: scion is inserted perpendicularly into stock and splits the otch cut;During grafting, the cambium layer outside scion and stock shape Stratification is directed at, and top shows money or valuables one carries unintentionally 5mm, is beneficial to scion and heals with stock.
(4) grafting post processing: bundle stock with grafting bar, infiltrates clean Cotton Gossypii pure water, is wrapped in grafting bar Periphery, then bagging;
(5) labelling: each scion PVC plastic is hung label and is marked;
(6) shelter from heat or light: be enclosed within outside bagging with kraft paper bag after labelling, carry out shading treatment;
(7) managing after grafting: after scion rudiment, progressively releases kraft paper bag and bagging.
Embodiment 1
Select 6 years raw Camellia sinensis of warmhouse booth to carry out Resources of Tea Plant food preservation test in base, Hunan Inst. of Tea's high bridge town, The semi-lignified branch of giving birth to then choosing its stalwartness is stock, tea tree breed Hunan ripple Hunan green, green that clip need to preserve early, sweet oak leaf Together, sword ripple is yellow, Hunan prince wife emerald green, early and the edible tender branch of rich 7 kinds of tender tea leaves is scion to high bridge, 3 scion of each kind grafting, uses Cut-grafting method carries out grafting, specifically comprises the following steps that
(1) stock prepares: the semi-lignified branch of giving birth to then choosing 6 years raw Camellia sinensis of warmhouse booth is stock, and branch is from bottom to top At about 20cm, stock level being cut off with secateurs, the intermediate vertical at stock cuts otch, and the degree of depth is about about 1cm;
(2) scion prepares: clip Hunan ripple Hunan green, green is early, sweet oak leaf is neat, sword ripple is yellow, Hunan prince wife is emerald green, high bridge early and rich 7 the Camellia sinensis product of tender tea leaves Planting and give birth to then without pest and disease damage, healthy and strong edible tender branch, clip bud head or a bud one short fringe of leaf are scion, and length is about 1.5cm, leaf Son cuts off half, and a cutter is respectively cut in scion bottom, forms wedge shape, and bud head wound is about 0.8cm, and a bud one leaf short fringe wound is long About 1cm, bevel is flat, straight.
(3) grafting: the otch at stock inserts scion, the cambium layer outside scion and stock cambium layer alignment, top is revealed White about 5mm.
(4) grafting post processing: carry out appropriateness binding with grafting bar after grafting, clean Cotton Gossypii pure water is infiltrated, parcel Peripheral at grafting bar, last bagging.
(5) labelling: each scion PVC plastic is hung label and is marked.
(6) shelter from heat or light: after grafting, be enclosed within bagging with kraft paper bag, carry out shading treatment.
(7) managing after grafting: after waiting 10d, scion and stock start healing, and scion starts rudiment, after 15d, and scion rudiment Growing about 1cm, after two months, progressively release kraft paper bag and bagging, survival rate is up to 90%.
Embodiment 2
Select 4 years raw Camellia sinensis of warmhouse booth to carry out Resources of Tea Plant food preservation test in base, Hunan Inst. of Tea's high bridge town, The semi-lignified branch of giving birth to then choosing its stalwartness is stock, tea tree breed Anji Ramulus et Folium Mussaendae Pubescentis that clip need to preserve, Chimonanthus salicifolius S.Y.Hu., green Hunan Early, sword ripple is yellow, the edible tender branch of Fuding white tea and green No. 26 kinds of Hunan ripple is scion, 3 scion of each kind grafting, adopts Grafting is carried out by cut-grafting method;Specifically comprise the following steps that
(1) stock prepares: the semi-lignified branch of giving birth to then choosing 4 years raw Camellia sinensis of warmhouse booth is stock, and branch is from bottom to top At about 15cm, stock level being cut off with secateurs, the intermediate vertical at anvil bar cuts a kerf, and the degree of depth is about about 1.2cm;
(2) scion prepares: Anji, clip Hunan Ramulus et Folium Mussaendae Pubescentis, Chimonanthus salicifolius S.Y.Hu., green Hunan are early, sword ripple is yellow, Fuding white tea and green No. 26 of Hunan ripple Giving birth to then without pest and disease damage, healthy and strong edible tender branch of tea tree breed, clip bud head or a bud one short fringe of leaf are scion, and length is about 1.5cm, leaf cuts off half, and a cutter is respectively cut in scion bottom, forms wedge shape, and bud head wound is about 0.8cm, and bud one leaf is short Fringe wound is about 1cm, and bevel is flat, straight.
(3) grafting: the otch at stock inserts scion, the cambium layer outside scion and stock cambium layer alignment, top is revealed White about 5mm.
(4) grafting post processing: carry out appropriateness binding with grafting bar after grafting, clean Cotton Gossypii pure water is infiltrated, parcel Peripheral at grafting bar, last bagging.
(5) labelling: each scion PVC plastic is hung label and is marked.
(6) shelter from heat or light: after grafting, be enclosed within bagging with kraft paper bag, carry out shading treatment.
(7) managing after grafting: after waiting 12d, scion and stock start healing, and scion starts rudiment, after 20d, and scion rudiment Growing about 1.1cm, after two months, progressively release kraft paper bag and bagging, survival rate is up to 90%.
The technical scheme provided the embodiment of the present invention above is described in detail, specific case used herein Principle and embodiment to the embodiment of the present invention are set forth, and the explanation of above example is only applicable to help to understand this The principle of inventive embodiments;Simultaneously for one of ordinary skill in the art, according to the embodiment of the present invention, in specific embodiment party All will change in formula and range of application, in sum, this specification content should not be construed as limitation of the present invention.

Claims (5)

1. the method that Tea Germplasm preserves, it comprises the following steps:
(1) selection of stock: stock need to select to be planted in the warmhouse booth Camellia sinensis of more than 3 years;
(2) stock prepares: choosing Camellia sinensis and giving birth to the branch of semi-lignified then is stock, carries out splitting cutting in stock horizontal cross-section;
(3) scion prepares: several tea tree breed edible tender branch that clip need to preserve respectively, the bud head of clip branch or bud one leaf Short fringe be scion;
(4) grafting: scion is inserted perpendicularly into stock and splits the otch cut;
(5) grafting post processing: bundle stock with grafting bar, infiltrates clean Cotton Gossypii pure water, is wrapped in the periphery of grafting bar, Bagging again;
(6) labelling: each scion PVC plastic is hung label and is marked;
(7) shelter from heat or light: be enclosed within outside bagging with kraft paper bag after labelling, carry out shading treatment;
(8) managing after grafting: after scion rudiment, progressively releases kraft paper bag and bagging.
The method that the most according to claim 1, Tea Germplasm preserves, it is characterised in that: when choosing stock, at stock certainly Cutting off by secateurs level at upper 10-20cm down, vertically cut otch in the centre position of stock, notch depth is 1-1.5cm.
The method that the most according to claim 2, Tea Germplasm preserves, it is characterised in that: a length of 1-of clip scion 2cm, scion bottom respectively cuts a cutter, forms wedge shape, and bevel is flat, straight.
The method that the most according to claim 3, Tea Germplasm preserves, it is characterised in that: when clip bud head is scion, its The long 0.6-0.8cm of bevel wound.
The method that the most according to claim 3, Tea Germplasm preserves, it is characterised in that: cut the short fringe taking bud one leaf Time, the long 1-1.5cm of wound, and leaf cuts off half.
CN201610597565.1A 2016-07-27 2016-07-27 A kind of method that Tea Germplasm preserves Pending CN106234036A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108040733A (en) * 2018-01-04 2018-05-18 湖北瑞晟生物有限责任公司 A kind of technology for the grafting success rate for improving Camellia nitidissima
CN108419556A (en) * 2018-06-20 2018-08-21 高方德 A kind of engrafting method of Jing-Qiu Pear
CN109417923A (en) * 2017-06-26 2019-03-05 聂后昌 The method of screening is covered in cleft grafting
CN110679314A (en) * 2019-06-03 2020-01-14 山东农业工程学院 Pepper grafting fixing device and using method
CN112913556A (en) * 2021-01-21 2021-06-08 中国农业科学院果树研究所 Fruit tree cultivation method beneficial to disease and insect prevention and control and improvement of fruit tree resource preservation efficiency
CN113692866A (en) * 2021-09-22 2021-11-26 禄劝普林生物科技有限责任公司 Method for grafting drupe twigs

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CN104885794A (en) * 2015-06-04 2015-09-09 贵州习水县勤韵茶业有限公司 Grafting method of wild tea camellias
CN104982232A (en) * 2015-06-19 2015-10-21 广西南亚热带农业科学研究所 Grafting and replanting method for tea tree
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CN104604538A (en) * 2015-01-27 2015-05-13 安徽农业大学 Method for performing positive and negative grafting on tea tree and tea-oil tree based on plumular axis grating technology
CN104885794A (en) * 2015-06-04 2015-09-09 贵州习水县勤韵茶业有限公司 Grafting method of wild tea camellias
CN104982232A (en) * 2015-06-19 2015-10-21 广西南亚热带农业科学研究所 Grafting and replanting method for tea tree
CN105532272A (en) * 2016-01-29 2016-05-04 广西南亚热带农业科学研究所 Grafting cultivation method of camellia chrysantha

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109417923A (en) * 2017-06-26 2019-03-05 聂后昌 The method of screening is covered in cleft grafting
CN108040733A (en) * 2018-01-04 2018-05-18 湖北瑞晟生物有限责任公司 A kind of technology for the grafting success rate for improving Camellia nitidissima
CN108419556A (en) * 2018-06-20 2018-08-21 高方德 A kind of engrafting method of Jing-Qiu Pear
CN110679314A (en) * 2019-06-03 2020-01-14 山东农业工程学院 Pepper grafting fixing device and using method
CN112913556A (en) * 2021-01-21 2021-06-08 中国农业科学院果树研究所 Fruit tree cultivation method beneficial to disease and insect prevention and control and improvement of fruit tree resource preservation efficiency
CN113692866A (en) * 2021-09-22 2021-11-26 禄劝普林生物科技有限责任公司 Method for grafting drupe twigs

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Application publication date: 20161221