CN106220319A - Production method containing the biological organic fertilizer addicted to sugar gemma bacillus agent - Google Patents
Production method containing the biological organic fertilizer addicted to sugar gemma bacillus agent Download PDFInfo
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- CN106220319A CN106220319A CN201610573946.6A CN201610573946A CN106220319A CN 106220319 A CN106220319 A CN 106220319A CN 201610573946 A CN201610573946 A CN 201610573946A CN 106220319 A CN106220319 A CN 106220319A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention relates to a kind of production method containing the biological organic fertilizer addicted to sugar gemma bacillus agent, the Sargassum after drying is clayed into power, and marine algae powder is added to the water according to certain ratio, after loading fermentation tank, and sterilizing under uniform temperature, certain time；Prepared by seed liquor: screening obtain a strain can degrade alignic fermentation strain YIC～ALG3 addicted to sugar bacillus cereus, be seeded to fluid medium cultivate certain time；By seed liquor according to certain mass than adding in fermentation tank, ferment under certain condition, after fermentation, adjust fermentation condition, after preserving certain time, again adjust fermentation condition and supplement seed liquor, obtaining addicted to sugar bacillus cereus after adjusting three times, pelletize after mixing with agricultural Organic substance addicted to sugar bacillus cereus, obtains containing the biological organic fertilizer addicted to sugar gemma bacillus agent.Sweat of the present invention is stablized controlled, and fermentation period is short, and product efficiency is notable, is suitable to large-scale production.
Description
Technical field
The present invention relates to the production method of a kind of biological organic fertilizer.
Background technology
Marine alga fertilizer, with natural seaweed as raw material, processes through special biochemical process, extracts and retains marine alga natural activity
Composition, a kind of novel organic fertilizer material that refine concentrates and formed.Marine alga fertilizer has raising crop yield and quality, resists disease pest
Effect that evil and poor environment and improvement soil conserve water and soil, it is applied to agricultural production has the huge market demand with good
Development prospect.But, the quality of marine alga fertilizer to be ensured, it is necessary for getting hold of Sargassum raw material and two crucial rings of extraction process
Joint.
First, the quality of marine alga fertilizer quality depends on raw material used.At present, the raw material used by alga fertilizer manufacturing enterprise
Main based on Brown algae, including yellow tang, Thallus Laminariae (Thallus Eckloniae), Thallus Laminariae (Thallus Eckloniae), Alga Sgrgassi Enerves etc..Other algae such as Fucus Vesiculosus, Entermorpha, ulva, Kappa
Zao Dengyeyou minority enterprise with.The external alga fertilizer enterprise overwhelming majority uses Brown algae to be raw material, the most especially applies with yellow tang
The most extensively such as famous brand names such as Seasol, Maxicrop, practical function effect also indicates that yellow tang is the Sargassum raw material of high-quality.
But, China coast does not has yellow tang to be distributed, and the raw material type that domestic enterprise uses is numerous and diverse, and quality is irregular to differ, and causes Sargassum
Fertilizer product quality is very different, and even some producer is turned on the camouflage of " alga fertilizer ", directly blends alginic acid, fulvic acid, nitrogen phosphorus
Potassium etc. pretend to be alga fertilizer, have impact on alga fertilizer quality, have upset alga fertilizer market.
Secondly, the second factor of decision alga fertilizer quality is extraction process.Different extraction processes is to alga fertilizer activity
The kind of composition and content impact are huge.At present, the extracting method of Sargassum active component mainly has chemical method, Physical and life
Thing method.Chemical method processes alginic cell mainly by acid, alkali and organic solvent, makes cell clear up or endogenous substance solubilising, should
Method is simple to operate, easily realizes, and is also the method for the most most alga fertilizer manufacturing enterprises employing.But, chemistry examination
Agent is sizable to the destruction of the active material ingredients in alginic cell, and the organic reagent remained also is potential to environment
Harm.The physical environmental condition such as the principle of Physical is to control pressure, temperature, utilize mechanical force to make alginic cell crush, interior
Tolerant release.Although the method technique cleaning, environmental friendliness, but the requirement for instrument and equipment is harsh, change in reaction conditions
Acutely, relatively costly, it is difficult to realize large-scale production.Bioanalysis includes enzymic degradation and two kinds of methods of fermentable, and it is main
Wanting principle is that to destroy alginic cell wall construction macromolecular substances of degrading be the small-molecule substance that plant easily absorbs, the party
Method reaction gentleness, product diversification, whole production process safety and environmental protection is pollution-free, is preferable alga fertilizer production method.
The research utilizing Production by Microorganism Fermentation marine alga fertilizer is relatively fewer, and only a few example researchs are that selection is multiple micro-
Biological carry out Sargassum raw material is similar to compost fermentation or aerobic fermentation process, there is sweat poor controllability, and fermentation period is long,
The problems such as target product is indefinite, it is difficult to meet the unification of industrialization production requirements, stable, controlled standard, the quality of product
It is difficult to ensure.
The microbial inoculum quantity in the purity of microbial inoculum and unit mass is mainly included for the evaluation index of agricultural micro organism.Cause
This, the bacterial preparation process as prior art is usually: carry out once under the conditions of the temperature suitably survived in microorganism and pH
Fermentation technology, is equivalent to hot-house culture.
Owing to the acidifying of current soil compaction is serious, outside air temperature is changeable, and the microbial bacterial agent of hot-house culture is directly thrown
Being put in soil, cause mortality because not adapting to severe soil environment, surviving because population cannot be formed on a small quantity even if having
Effect, also cannot play the effect of microbial bacterial agent.
Summary of the invention
The technical problem to be solved is to provide a kind of containing the biological organic fertilizer addicted to sugar gemma bacillus agent
Production method, first, use function admirable be used for degrade alignic addicted to sugar bacillus cereus degraded Sargassum, and simulate soil from
So ecological environment, make after fermentation addicted to sugar bacillus cereus after putting into soil, can quickly adapt to environment, it is ensured that survival rate；The second,
There is provided and prepare addicted to sugar bacillus cereus with containing the seaweed bio fertilizer addicted to sugared bacillus cereus addicted to sugar bacillus cereus described in utilizing
Method, the method sweat is stablized controlled, and fermentation period is short, and product efficiency is notable, is suitable to large-scale production.
In order to solve above-mentioned technical problem, the present invention by the following technical solutions:
A kind of production method containing the biological organic fertilizer addicted to sugar gemma bacillus agent, it is characterised in that according to following steps
Carry out:
By agricultural Organic substance and addicted to sugar gemma bacillus agent according to mass ratio 40～99: 1～60 mix after pelletize, obtain
Containing the biological organic fertilizer addicted to sugar gemma bacillus agent；
Two kinds of a kind of or arbitrary proportion during wherein agricultural Organic substance is ammonification humic acids, furfural dregs and mushroom residue with
On；
Wherein it is prepared from according to following steps addicted to sugar gemma bacillus agent:
1), marine algae powder is prepared: the Sargassum after drying is clayed into power；
2), preparation product A: marine algae powder loads in fermentation tank with water according to mass ratio 1～10: 100, at 100～150 DEG C
Under the conditions of sterilizing 15～35min, obtain product A；
And prepare seed liquor: fluid medium will be seeded to addicted to sugar bacillus cereus and cultivate 20～30 hours；
It is 1～10: 100 addicted to the volume ratio between sugar bacillus cereus and culture medium；
3), preparation product B: seed liquor is joined in product A and obtain product B, wherein seed liquor and the mass ratio of product A
It is 1～10: 100；
4), preparation products C: be 20 DEG C～40 DEG C in temperature, under conditions of pH5～9, product B fermented, during fermentation
Between be 6～10 days, obtain products C；
5) product D, is prepared: adjust the pH to 5.0～6.0 of products C, temperature to 3～10 DEG C, preserve 3h～72h, produced
Product D；
6), preparation product E: adjust product D temperature to 20 DEG C～40 DEG C, pH to 5～9, then supplement seed liquor, supplement
The mass ratio of seed liquor and product D be 1～10: 100, preserve 3h～72h, obtain product E；
7), preparation product F: adjust the pH to 5.0～6.0 of product E, temperature to 3～10 DEG C, preserve 3h～72h, produced
Product F；
8), preparation product G: adjust product F temperature to 20 DEG C～40 DEG C, pH to 5～9, then supplement seed liquor, supplement
The mass ratio of seed liquor and product F be 1～10: 100, preserve 3h～72h, obtain product G；
9), preparation is addicted to sugar gemma bacillus agent: obtain after product G being air-dried addicted to sugar gemma bacillus agent.
The pH7.2～7.4 of described fluid medium, and be made into according to following mass ratio: sodium alginate 5 parts, ammonium sulfate 5
Part, 1 part of magnesium sulfate, dipotassium hydrogen phosphate 2 parts, 0.01 part of ferrous sulfate, 20 parts of agar and the mixture of distilled water 1000 parts.
Step 2) mass ratio of described marine algae powder and water is 4: 100；Sterilising temp 115 DEG C, sterilization time 25min；System
During standby seed liquor, it is seeded in fluid medium addicted to sugar bacillus cereus, cultivates 22 hours, obtain seed liquor, addicted to sugar bacillus cereus
It is 5: 100 with the volume ratio of culture medium；Step 5) and step 7) in, temperature is 5 DEG C, and the holding time is 12h.
Step 4), step 6) and step 8) in, temperature is 25 DEG C, and pH is 6.5～7.0；Step 4) fermentation time is 8 days；
Step 6) and step 8) holding time is 12h；Step 6) in the mass ratio of fermentation seed liquid and product D and step 8) in fermentation
Seed liquor is 5: 100 with the mass ratio of product F.
Step 5), step 6) step 7) and step 8) in, adjust temperature be per hour adjust 3 DEG C.
Described Sargassum refers to the two or more of a kind of or arbitrary proportion in sargassum thunbergii, Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) and Alga Sgrgassi Enerves.
Described is that its preserving number is CGMCC No.12155 addicted to sugar bacillus cereus YIC～Alg3 addicted to sugar bacillus cereus.
Invent and there is following Advantageous Effects:
The present invention by screening obtain a strain marine source addicted to sugar bacillus cereus, this bacterial strain have degraded alignic energy
Power, alginic acid of on the one hand can degrading generates the brown alga oligose etc. of small-molecular-weight and is prone to the nutritional labeling that plant absorption utilizes；Separately
On the one hand destroy more nutrient substance in alginic cell wall construction promotes Sargassum body to be released.This strain fermentation is utilized to process copper
The Sargassum Sargassum raw material such as algae, sargassum thunbergii, can detect that in fermentation liquid after certain natural law that ferments molecular weight 4000Da with
Under, the degree of polymerization be the brown alga oligose of 2-20, using this fermentation liquid as alga fertilizer mother solution, direct or compounding N, P, K, trace unit
Element, aminoacid, the effective ingredient such as organic is for crop growth.Fertilizer efficiency experiment shows, utilizes the method to obtain fermentation liquid energy
Enough it is obviously promoted plant root growth, improves Soil Micro-environment, improve crop yield.
The present invention also has the following characteristics that
1, use the marine source that obtains of the present invention addicted to sugar bacillus cereus YIC～Alg3 to fermentation of seaweed, sweat
Stablizing controlled, fermentation period is short, and product efficiency is notable, is suitable to large-scale production.
What 2, the present invention obtained has good water solubility addicted to sugar bacillus cereus, it is easy to plant absorption utilizes, simultaneously as one
Important signaling molecule, it is possible to the growth regulating of involved in plant and induction resistance mechanism promote the growth of plant, improve plant pair
Poor environment and the resistance of pest and disease damage.
3, the present invention is regulated by temperature slowly, makes the change addicted to sugar bacillus cereus adaptive temperature, it is to avoid variations in temperature
Too fast, do not adapt to and mortality addicted to sugar bacillus cereus.
What 4, the present invention obtained has outside Sargassum extract feature addicted to sugar bacillus cereus, also has stronger environment and adapts to
Property, in the soil put into acidifying and in the environment of variations in temperature impermanence, can quickly adapt to environment survival, play microbial bacteria
The effect of agent.
Accompanying drawing explanation
Fig. 1 is microexamination YIC～the Gram’s staining of Alg3 bacterial strain and assembles agglomerating phenomenon figure.
Fig. 2 is YIC～Alg3 bacterial strain phylogenetic tree schematic diagram based on 16SrDNA sequence construct.
Detailed description of the invention
The present invention is further illustrated below in conjunction with instantiation.
Embodiment 1
Fresh sargassum thunbergii after clean drying is worn into 50～60 mesh powder；
Marine algae powder loads in fermentation tank according to mass ratio 2: 100 with water, and under the conditions of 115 DEG C, sterilizing 35min, is produced
Product A；And prepare seed liquor: fluid medium will be seeded to addicted to sugar bacillus cereus YIC～Alg3 and cultivate 20 hours；Described addicted to sugar bud
The preserving number of spore bacillus YIC～Alg3 is CGMCC No.12155；Described fluid medium is: sodium alginate 5g, ammonium sulfate 5g,
Magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar 20g and the mixture of distilled water 1000ml, pH7.2～7.4；
Volume ratio addicted to sugar bacillus cereus YIC～Alg3 and culture medium is 2: 100.Seed liquor is joined in product A, wherein seed
Liquid is 1: 100 with the mass ratio of product A, obtains product B；It is 20 DEG C in temperature, under conditions of pH5.5～6.0, product B is carried out
Fermentation, fermentation time is 7 days, obtains products C；Adjust the pH to 5.0～6.0 of products C, and with the variations in temperature of per hour 20 DEG C
Adjustment temperature, to 5 DEG C, preserves 24h, obtains product D；The temperature of product D is adjusted to 20 DEG C with the variations in temperature of per hour 20 DEG C,
PH to 5.5～6.0, then supplements seed liquor, and supplementary seed liquor is 1: 100 with the mass ratio of product D, preserves 24h, is produced
Product E；Adjust the pH to 5.0～6.0 of product E, and adjust temperature to 5 DEG C with the variations in temperature of per hour 20 DEG C, preserve
24h, obtains product F；
The temperature of product F is adjusted to 20 DEG C with the variations in temperature of per hour 20 DEG C, pH to 5.5～6.0, then supplements seed
Liquid, supplementary seed liquor is 1: 100 with the mass ratio of product F, preserves 24h, obtains product G；By product G air-dry after obtain addicted to
Sugar bacillus cereus YIC～Alg3；By agricultural Organic substance and addicted to sugar bacillus cereus YIC～Alg3 according to mass ratio 95: 5 mix after
Pelletize, obtains containing the biological organic fertilizer addicted to sugar gemma bacillus agent.
Embodiment 2
Fresh Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) after clean drying and new fresh sargassum are compounded according to mass ratio 1: 1, wears into 50～60 mesh powder；
Marine algae powder loads in fermentation tank according to mass ratio 4: 100 with water, and under the conditions of 115 DEG C, sterilizing 25min, is produced
Product A；And prepare seed liquor: fluid medium will be seeded to addicted to sugar bacillus cereus YIC～Alg3 and cultivate 22 hours；Described addicted to sugar bud
The preserving number of spore bacillus YIC～Alg3 is CGMCC No.12155；Described fluid medium is: sodium alginate 5g, ammonium sulfate 5g,
Magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar 20g and the mixture of distilled water 1000ml, pH7.2～7.4；
Volume ratio addicted to sugar bacillus cereus YIC～Alg3 and culture medium is 5: 100.Seed liquor is joined in product A, wherein seed
Liquid is 5: 100 with the mass ratio of product A, obtains product B；It is 25 DEG C in temperature, under conditions of pH6.5～7.0, product B is carried out
Fermentation, fermentation time is 8 days, obtains products C；Adjust the pH to 5.0～6.0 of products C, and with the variations in temperature of per hour 3 DEG C
Adjustment temperature, to 5 DEG C, preserves 12h, obtains product D；The temperature of product D is adjusted to 25 DEG C, pH with the variations in temperature of per hour 3 DEG C
To 6.5～7.0, then supplementing seed liquor, supplementary seed liquor is 5: 100 with the mass ratio of product D, preserves 12h, obtains product
E；Adjust the pH to 5.0～6.0 of product E, and adjust temperature to 5 DEG C with the variations in temperature of per hour 3 DEG C, preserve 12h,
To product F；
The temperature of product F is adjusted to 25 DEG C with the variations in temperature of per hour 3 DEG C, pH to 6.5～7.0, then supplements seed
Liquid, supplementary seed liquor is 5: 100 with the mass ratio of product F, preserves 12h, obtains product G；Obtain addicted to sugar after product G is air-dried
Bacillus cereus YIC～Alg3；Make by agricultural Organic substance with addicted to sugar bacillus cereus YIC～Alg3 according to after mass ratio 95: 5 mixing
Grain, obtains containing the biological organic fertilizer addicted to sugar gemma bacillus agent.
Embodiment 3
New fresh sargassum after clean drying is worn into 50～60 mesh powder；
Marine algae powder loads in fermentation tank according to mass ratio 6: 100 with water, and under the conditions of 125 DEG C, sterilizing 35min, is produced
Product A；And prepare seed liquor: fluid medium will be seeded to addicted to sugar bacillus cereus YIC～Alg3 and cultivate 24 hours；Described addicted to sugar bud
The preserving number of spore bacillus YIC～Alg3 is CGMCC No.12155；Described fluid medium is: sodium alginate 5g, ammonium sulfate 5g,
Magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar 20g and the mixture of distilled water 1000ml, pH7.2～7.4；
Volume ratio addicted to sugar bacillus cereus YIC～Alg3 and culture medium is 8: 100.Seed liquor is joined in product A, wherein seed
Liquid is 4: 100 with the mass ratio of product A, obtains product B；It is 30 DEG C in temperature, carries out product B under conditions of pH7～7.5 sending out
Ferment, fermentation time is 9 days, obtains products C；Adjust the pH to 5.0～6.0 of products C, and adjust with the variations in temperature of per hour 1 DEG C
Whole temperature, to 5 DEG C, preserves 18h, obtains product D；Adjusting the temperature of product D to 30 DEG C with the variations in temperature of per hour 8 DEG C, pH is extremely
7～7.5, then supplement seed liquor, supplementary seed liquor is 4: 100 with the mass ratio of product D, preserves 18h, obtains product E；
Adjust the pH to 5.0～6.0 of product E, and adjust temperature to 5 DEG C with the variations in temperature of per hour 1 DEG C, preserve 18h, obtain
Product F；
The temperature of product F is adjusted to 30 DEG C with the variations in temperature of per hour 8 DEG C, pH to 7.0～7.5, then supplements seed
Liquid, supplementary seed liquor is 4: 100 with the mass ratio of product F, preserves 18h, obtains product G；Obtain addicted to sugar after product G is air-dried
Bacillus cereus YIC～Alg3；Make by agricultural Organic substance with addicted to sugar bacillus cereus YIC～Alg3 according to after mass ratio 95: 5 mixing
Grain, obtains containing the biological organic fertilizer addicted to sugar gemma bacillus agent.
Embodiment 4
New fresh sargassum after clean drying is worn into 50～60 mesh powder；
Marine algae powder loads in fermentation tank according to mass ratio 9: 100 with water, and under the conditions of 140 DEG C, sterilizing 15min, is produced
Product A；And prepare seed liquor: fluid medium will be seeded to addicted to sugar bacillus cereus YIC～Alg3 and cultivate 20 hours；Described addicted to sugar bud
The preserving number of spore bacillus YIC～Alg3 is CGMCC No.12155；Described fluid medium is: sodium alginate 5g, ammonium sulfate 5g,
Magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g, agar 20g and the mixture of distilled water 1000ml, pH7.2～7.4；
Volume ratio addicted to sugar bacillus cereus YIC～Alg3 and culture medium is 5: 100.Seed liquor is joined in product A, wherein seed
Liquid is 8: 100 with the mass ratio of product A, obtains product B；It is 35 DEG C in temperature, under conditions of pH6.5～7.0, product B is carried out
Fermentation, fermentation time is to obtain products C in 9 days；Adjust the pH to 5.0～6.0 of products C, and adjust with the variations in temperature of per hour 3 DEG C
Whole temperature, to 5 DEG C, preserves 12h, obtains product D；Adjusting the temperature of product D to 35 DEG C with the variations in temperature of per hour 3 DEG C, pH is extremely
6.5～7.0, then supplement seed liquor, supplementary seed liquor is 8: 100 with the mass ratio of product D, preserves 12h, obtains product E；
Adjust the pH to 5.0～6.0 of product E, and adjust temperature to 5 DEG C with the variations in temperature of per hour 3 DEG C, preserve 12h, obtain
Product F；
The temperature of product F is adjusted to 35 DEG C with the variations in temperature of per hour 3 DEG C, pH to 6.5～7.0, then supplements seed
Liquid, supplementary seed liquor is 8: 100 with the mass ratio of product F, preserves 12h, obtains product G；Obtain addicted to sugar after product G is air-dried
Bacillus cereus YIC～Alg3；Make by agricultural Organic substance with addicted to sugar bacillus cereus YIC～Alg3 according to after mass ratio 95: 5 mixing
Grain, obtains containing the biological organic fertilizer addicted to sugar gemma bacillus agent.
In embodiment 1 to 4, described agricultural Organic substance is furfural dregs.
1 materials and methods:
1.1 test sites: Wuzhoufeng Agricultural Technology Co., Ltd. proving ground, neutral brown earth, middle high fertility.
1.2 tomato varieties: tobacco powder 2.
1.3 for examination fertilizer: seaweed bio fertilizer: addicted to sugar bacillus cereus >=200,000,000/gram, organic >=60%, N+P2O5+
K2O >=6%, is provided by Wuzhoufeng Agricultural Technology Co., Ltd.；Common fertilizer: organic >=60%, N+P2O5+K2O >=6%,
Thered is provided by Wuzhoufeng Agricultural Technology Co., Ltd.；15-15-15 NPK: N >=15%, P2O5>=15%, K2O >=15%,
Thered is provided by Wuzhoufeng Agricultural Technology Co., Ltd..
1.4 EXPERIMENTAL DESIGN: test and set 6 process:
Process 1 (CK1): common fertilizer；
Process 2 (CK2): add the seaweed bio fertilizer addicted to sugar bacillus cereus not adjusting temperature and pH；
Process 3 (T1): seaweed bio fertilizer described in embodiment 1；
Process 4 (T2): seaweed bio fertilizer described in embodiment 2；
Process 5 (T3): seaweed bio fertilizer described in embodiment 3；
Process 6 (T4): seaweed bio fertilizer described in embodiment 4.
Each process repeats for 3 times, and random district group arranges, plot area 15m2, field planting 75 strain, if protection row.Community is single receives
Calculate yield respectively.
1.5 fertilizing methods:
300kg biological organic fertilizer is executed in each process 1 mu and base manure made by 100kg15-15-15 NPK, and other management are same
Conventional.
2 results and analysis
2.1 Fructus Lycopersici esculenti different fertilization fertilizer effects are analyzed
As can be seen from Table 1, each seaweed bio fertilizer, compared with the process of common fertilizer, all has increasing in various degree
Produce.Wherein embodiment 2 processes amount of increase in production maximum, mu volume increase 1169.0kg, amplification 16.3%；Several embodiments are not adjusted with adding
Whole temperature compares, with the process addicted to the seaweed bio fertilizer of sugar bacillus cereus of pH, the effect of increasing production also having in various degree.Totally
For, the yield order of several process is T2 ＞ T3 ＞ T4 ＞ T1 ＞ CK2 ＞ CK1.
The fertile impact on tomato yield of table 1 seaweed bio machine
Addicted to the activity analysis of sugar bacillus cereus in 2.2 different fertilization soil
Addicted to sugar bacillus cereus quantity in table 2 different disposal soil
105CFU/g
As shown in table 2, apply after adding the seaweed bio fertilizer addicted to sugar bacillus cereus, topsoil soils has different number
Amount addicted to sugar bacillus cereus survival, and amount of survival and tomato yield correlation.Wherein embodiment 2 processes in soil
Most addicted to sugar bacillus cereus quantity, reach 10.6*105CFU/g, T3, T4, T1, CK2 successively decrease successively thereafter.
Preserving number addicted to sugar bacillus cereus YIC～Alg3 of the present invention is CGMCC No.12155, depositary institution's title:
China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution address: BeiChen West Road, Chaoyang District, BeiJing City 1
Number institute 3, preservation date: on 03 02nd, 2016, Latin Classification And Nomenclature: Bacillus halosaccharovorans.
The described biological nature addicted to sugar bacillus cereus YIC～Alg3 is as follows:
After YIC～Alg3 bacterial strain grows 48h on alginic acid sole carbon source flat board, bacterium colony is irregular, and faint yellow, centre is not
Transparent, edge is translucent, surface folding.It is shaft-like that microscope observes bacterial strain, can produce spore, gram at end or proximal end
The positive, can move, and Later growth thalline easily assembles agglomerating (Fig. 1).Growth temperature range 20～45 DEG C, PH scope 6～9, NaCl
Concentration range 0～15%, bacterial strain optimum growth temperature 37 DEG C, the most suitable growth pH 7.5.Catalase, oxidase, VP tests the positive, energy
Enough utilize glucose, mannose, mannitol, fructose, lactose, galactose, starch；Can not utilize sorbitol, cellulose,.Bacterial strain
16SrDNA sequence sees appendix 1, and phylogenetic tree is as shown in Figure 2.According to its physiological and biochemical property, 16SrDNA sequence and system are sent out
Educate tree information, be addicted to sugar bacillus halosaccharovorans by this identification of strains.
Note :+represent and can utilize ,～represent and can not utilize.
Claims (7)
1. the production method containing the biological organic fertilizer addicted to sugar gemma bacillus agent, it is characterised in that enter according to following steps
OK:
By agricultural Organic substance and addicted to sugar gemma bacillus agent according to mass ratio 40～99:1～60 mix after pelletize, contained
Biological organic fertilizer addicted to sugar gemma bacillus agent；
A kind of or arbitrary proportion during wherein agricultural Organic substance is ammonification humic acids, furfural dregs and mushroom residue two or more；
Wherein it is prepared from according to following steps addicted to sugar gemma bacillus agent:
1), marine algae powder is prepared: the Sargassum after drying is clayed into power；
2), preparation product A: marine algae powder loads in fermentation tank with water according to mass ratio 1～10:100,100～150 DEG C of conditions
Lower sterilizing 15～35min, obtains product A；
And prepare seed liquor: fluid medium will be seeded to addicted to sugar bacillus cereus and cultivate 20～30 hours；
It is 1～10:100 addicted to the volume ratio between sugar bacillus cereus and culture medium；
3), preparation product B: seed liquor being joined in product A and obtain product B, wherein seed liquor is 1 with the mass ratio of product A
～10:100；
4), preparation products C: being 20 DEG C～40 DEG C in temperature, under conditions of pH5～9 ferment product B, fermentation time is 6
～10 days, obtain products C；
5) product D, is prepared: adjust the pH to 5.0～6.0 of products C, temperature to 3～10 DEG C, preserve 3h～72h, obtain product D；
6), preparation product E: adjust product D temperature to 20 DEG C～40 DEG C, pH to 5～9, then supplement seed liquor, supplementary kind
Sub-liquid is 1～10:100 with the mass ratio of product D, preserves 3h～72h, obtains product E；
7), preparation product F: adjust the pH to 5.0～6.0 of product E, temperature to 3～10 DEG C, preserve 3h～72h, obtain product F；
8), preparation product G: adjust product F temperature to 20 DEG C～40 DEG C, pH to 5～9, then supplement seed liquor, supplementary kind
Sub-liquid is 1～10:100 with the mass ratio of product F, preserves 3h～72h, obtains product G；
9), preparation is addicted to sugar gemma bacillus agent: obtain after product G being air-dried addicted to sugar gemma bacillus agent.
2. the production method containing the biological organic fertilizer addicted to sugar gemma bacillus agent as claimed in claim 1, it is characterised in that:
The pH7.2～7.4 of described fluid medium, and be made into according to following mass ratio: sodium alginate 5 parts, 5 parts of ammonium sulfate, magnesium sulfate 1
Part, dipotassium hydrogen phosphate 2 parts, 0.01 part of ferrous sulfate, 20 parts of agar and the mixture of distilled water 1000 parts.
3. the production method containing the biological organic fertilizer addicted to sugar gemma bacillus agent as claimed in claim 1, it is characterised in that:
Step 2) mass ratio of described marine algae powder and water is 4:100；Sterilising temp 115 DEG C, sterilization time 25min；Preparation seed liquor
Time, it is seeded in fluid medium addicted to sugar bacillus cereus, cultivates 22 hours, obtain seed liquor, addicted to sugar bacillus cereus and culture medium
Volume ratio be 5:100；In step 5) and step 7), temperature is 5 DEG C, and the holding time is 12h.
4. the production method containing the biological organic fertilizer addicted to sugar gemma bacillus agent as claimed in claim 1, it is characterised in that:
In step 4), step 6) and step 8), temperature is 25 DEG C, and pH is 6.5～7.0；Step 4) fermentation time is 8 days；Step 6) and
The step 8) holding time is 12h；In step 6) in the mass ratio of fermentation seed liquid and product D and step 8) fermentation seed liquid with
The mass ratio of product F is 5:100.
5. the production method containing the biological organic fertilizer addicted to sugar gemma bacillus agent as claimed in claim 1, it is characterised in that:
In step 5), step 6) step 7) and step 8), adjust temperature and be adjustment 3 DEG C per hour.
6. the production method containing the biological organic fertilizer addicted to sugar gemma bacillus agent as claimed in claim 1, it is characterised in that:
Described Sargassum refers to the two or more of a kind of or arbitrary proportion in sargassum thunbergii, Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) and Alga Sgrgassi Enerves.
7. as claimed in claim 1 containing addicted to sugar bacillus cereus and the production method of amino acid whose Water soluble fertilizer, it is characterised in that:
Described is that its preserving number is CGMCC No.12155 addicted to sugar bacillus cereus YIC～Alg3 addicted to sugar bacillus cereus.
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| CN104447008A (en) * | 2014-12-05 | 2015-03-25 | 中国科学院烟台海岸带研究所 | Method for preparing liquid seaweed fertilizer |
| CN104774094A (en) * | 2015-04-01 | 2015-07-15 | 大连理工大学 | Preparation method of compound microbial seaweed fertilizer |
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| CN102174430A (en) * | 2010-12-31 | 2011-09-07 | 中国海洋大学生物工程开发有限公司 | Bacillus psychrosaccharolyticus strain and application thereof in complex microorganism seaweed fertilizer |
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Application publication date: 20161214 |