CN106215993B - Sterile medical centrifuge tube and preparation method thereof for preparing platelet-rich plasma - Google Patents
Sterile medical centrifuge tube and preparation method thereof for preparing platelet-rich plasma Download PDFInfo
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- CN106215993B CN106215993B CN201610597174.XA CN201610597174A CN106215993B CN 106215993 B CN106215993 B CN 106215993B CN 201610597174 A CN201610597174 A CN 201610597174A CN 106215993 B CN106215993 B CN 106215993B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
- B01L3/50825—Closing or opening means, corks, bungs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides a sterile medical centrifuge tube, which comprises a centrifuge tube body, a tube cover arranged at the tube orifice of the centrifuge tube body, a liquid inlet/waste liquid discharge tube and a suction tube arranged in the centrifuge tube body; the liquid inlet/waste liquid discharge pipe is characterized in that an opening and a hole cover for sealing or opening the opening are arranged on the pipe cover, one end of the liquid inlet/waste liquid discharge pipe is communicated with the opening, the other end of the liquid inlet/waste liquid discharge pipe is contacted with or is close to the bottom of the centrifuge pipe body, one end of the suction pipe is communicated with the opening, the other end of the suction pipe is close to the bottom of the centrifuge pipe body and slightly higher than the bottom of the liquid inlet/waste liquid discharge pipe, and a selection knob capable of selecting the opening to be connected with the liquid inlet/waste liquid discharge pipe or the suction pipe is arranged above the pipe cover. The invention also provides a preparation method of the platelet-rich plasma. The aseptic medical centrifuge tube is more convenient to operate, more in preparation types, wider in application range, stronger in universality and stronger in sterility.
Description
Technical Field
The invention relates to a medical instrument, in particular to a sterile medical centrifuge tube and a preparation method for preparing platelet-rich plasma by using the same.
Background
Platelet Rich Plasma (PRP) is isolated from blood and contains various growth factors such as transforming growth factor- β (transforming growth factor, TGF), platelet-derived growth factor (platelet-derived growth factor, PDGF), insulin-like growth factor (insulin-like growth factor, IGF), vascular endothelial growth factor (vascular endothelial growth factor, VEGF), and in addition, leukocytes and fibrin. Several studies have shown that PRP is significant in soft tissue repair, and can promote cartilage regeneration while slowing down cartilage degeneration. Thus, PRP has now become a hot topic in the current field of cartilage repair.
In fact, the requirements for PRP for repair are different for different tissues in the human body, e.g. leukocyte-rich PRP is suitable for most tissues but not for intra-articular use. Thus, an apparatus capable of performing strict PRP production according to various needs is highly demanded in the art. In addition to the defects of single product preparation, inflexible use, limited PRP extraction mode and the like, the existing ten-item PRP preparation equipment which passes the American FDA certification also has other various defects including high price, complicated centrifugation times, low platelet collection rate and the like. However, similar products sold in domestic markets have various limitations, such as products designed to be only aimed at wounds, and single products are prepared; and due to design problems, blood is sucked and injected through the same suction pipe, so that excessive red blood cells and other impurities remain, and the stability of PRP preparation is affected.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides a sterile medical centrifuge tube and a preparation method for preparing platelet-rich plasma by using the same, wherein the sterile medical centrifuge tube is more convenient to operate, more in preparation types, wider in application range, stronger in universality and stronger in sterility.
The invention relates to a sterile medical centrifuge tube, which comprises a centrifuge tube body, a tube cover arranged at the tube orifice of the centrifuge tube body, a liquid inlet/waste liquid discharge tube and a suction tube arranged in the centrifuge tube body; the liquid inlet/waste liquid discharge pipe is characterized in that an opening and a hole cover for sealing or opening the opening are arranged on the pipe cover, one end of the liquid inlet/waste liquid discharge pipe is communicated with the opening, the other end of the liquid inlet/waste liquid discharge pipe is contacted with or is close to the bottom of the centrifuge pipe body, one end of the suction pipe is communicated with the opening, the other end of the suction pipe is close to the bottom of the centrifuge pipe body and slightly higher than the bottom of the liquid inlet/waste liquid discharge pipe, and a selection knob capable of selecting the opening to be connected with the liquid inlet/waste liquid discharge pipe or the suction pipe is arranged above the pipe cover.
Further, the opening is provided at a central position of the tube cover, so that the suction tube can suck the platelet part formed after centrifugation to the maximum extent.
The open hole is divided into an inner layer and an outer layer, the outer side of the outer layer is provided with threads or an embedded ring, the threads or the embedded ring are matched with the inner side threads or the inner side embedded rings of the hole cover to form threads or embedded connection, the inner layer is an injector interface, and the inner layer is embedded or in threaded connection with a common injector or a threaded injector; further, a gap of 0.7-2.5mm is reserved between the inner layer and the outer layer, and the gap is covered with a permeable water sealing filtering membrane; a rigid connection exists between the inner layer and the outer layer. The hole covers can be tightly connected, and as the stress of each component is huge in the centrifugation process, liquid leakage or shaking can be prevented in the centrifugation process; in addition, the ventilation part and the liquid inlet and outlet part are arranged in the same opening and covered by the same hole cover, so that the centrifuge tube is completely sealed in the centrifugation process, and the sterility of the inside of the centrifugation process is ensured.
Further, the pipe diameters of the liquid inlet/waste liquid discharge pipe and the suction pipe are 0.3-1.2mm, and the bottom ends of the liquid inlet/waste liquid discharge pipe and the suction pipe are inclined planes. The smaller pipe diameter ensures that the residual quantity of red blood cells is smaller, and the liquid level control degree is better when the operator absorbs the red blood cells.
Further, the bottom end of the suction pipe is 0.5-1mm higher than the bottom end of the liquid inlet/waste liquid discharge pipe, the suction pipe and the liquid inlet/waste liquid discharge pipe are symmetrically positioned at two sides of the center shaft and mutually adjacent, can be integrally formed, and are rigidly connected by the same material in the middle. Therefore, when the platelet part is sucked through the suction tube, the platelet part can not be touched, the interference of red blood cells is reduced as much as possible, and the precision and purity of sucking PRP are ensured. In addition, the suction pipe does not pass through any liquid before finally sucking the PRP, and thus, no other components remain.
Further, densely-protruding vertical lines or longitudinally-protruding strips for increasing rotation friction are arranged on the outer side of the selection knob; the inside of the liquid inlet/waste liquid outlet pipe is provided with a fixing protrusion, so that the liquid inlet/waste liquid outlet pipe can be fixed at two positions, the two positions enable the hole cover to be communicated with the liquid inlet/waste liquid outlet pipe and the suction pipe respectively, and two sides of the fixing protrusion are provided with fixing plates for preventing excessive rotation so as to limit the rotation range of the fixing plates.
Further, the bottom of the centrifuge tube body is a symmetrical reverse cone.
Further, the centrifuge tube body is manufactured into appliances with various specifications within the range of 5-100mL and is provided with standard graduation marks.
Further, the centrifuge tube body is tightly connected with the tube cover and cannot be separated. Therefore, the problem that the tube cover is separated or opened by mistake in actual operation can be avoided, and the blood is polluted by the external environment is avoided.
The subject of the invention is also a process for the preparation of platelet rich plasma by means of a sterile medical centrifuge tube as described in any of the preceding claims.
Further, the liquid inlet/waste liquid discharge pipe is positioned at the center of the centrifuge tube, is vertical and extends to the bottom end of the centrifuge tube; the suction pipe is adjacent to the liquid inlet/waste liquid discharge pipe and slightly shorter than the liquid inlet/waste liquid discharge pipe; one end of the opening can be tightly communicated with the injector, the injector is wrapped by the inner diameter, the injector is inserted in a seamless way, one end of the opening is communicated with the liquid inlet/waste liquid discharge pipe or the suction pipe, a selection knob below the opening is used for selecting whether the opening is communicated with the liquid inlet/waste liquid discharge pipe or the suction pipe, and an external spiral cover can be used for sealing the opening above the opening
In one embodiment, the preparation method uses a secondary centrifugation method to prepare the leukocyte-enriched PRP, and the specific steps include:
step 1: after the blood of a patient is subjected to centrifugal layering in a sterile medical centrifuge tube, inserting a syringe into an opening, adjusting a selection knob to a hole cover to be communicated with a liquid inlet/waste liquid discharge pipe, and sucking a bottom erythrocyte layer from the liquid inlet/waste liquid discharge pipe;
step 2: centrifuging again, inserting the injector into the opening, adjusting the selection knob to the hole cover to be communicated with the liquid inlet/waste liquid discharge pipe, and sucking out the residual red blood cell layer at the bottom layer from the liquid inlet/waste liquid discharge pipe;
step 3: after the suction is finished, the selection knob is adjusted to the hole cover to be communicated with the suction pipe, and the blood intermediate layer (rich in white blood cells and platelets) and the components below the plasma layer are sucked from the suction pipe to obtain the white blood cell-rich PRP.
In one embodiment, the preparation method uses a one-time centrifugation method to prepare the leukocyte-depleted PRP, and the specific steps include:
step 1: after the blood of a patient is centrifugally layered in a sterile medical centrifuge tube, inserting a syringe into the opening, adjusting a selection knob to a hole cover to be communicated with a liquid inlet/waste liquid discharge pipe, and sucking out a red blood cell layer and a blood middle layer (rich in white blood cells) of the bottom layer from the liquid inlet/waste liquid discharge pipe;
step 2: and after the suction is finished, adjusting the selection knob to the hole cover to be communicated with the suction pipe, and sucking components below the plasma layer from the suction pipe to obtain the lean white blood cell PRP.
In one embodiment, the preparation method uses a secondary centrifugation method to prepare the leukocyte-depleted PRP, and the specific steps include:
step 1: after the blood of a patient is centrifugally layered in a sterile medical centrifuge tube, inserting a syringe into the opening, adjusting a selection knob to a hole cover to be communicated with a liquid inlet/waste liquid discharge pipe, and sucking out a red blood cell layer and a blood middle layer (rich in white blood cells) of the bottom layer from the liquid inlet/waste liquid discharge pipe;
step 2: centrifuging again, inserting the injector into the opening, adjusting the selection knob to the hole cover to be communicated with the liquid inlet/waste liquid discharge pipe, and sucking out the residual red blood cell layer at the bottom layer from the liquid inlet/waste liquid discharge pipe;
step 3: after the sucking is finished, the selection knob is adjusted to the hole cover to be communicated with the sucking pipe, and the components below the middle layer (rich in platelets) and the plasma layer of blood are sucked from the sucking pipe to obtain the lean white blood cell PRP.
Compared with the prior art, the invention has the beneficial effects that: 1. the invention is suitable for preparing various plasma centrifugal products such as lean leukocyte PRP, rich leukocyte PRP and the like; 2. the invention prepares a plurality of PRPs, has wider PRP indications, and is suitable for various diseases such as cartilage defect, osteoarthritis, wound surface, ligament injury, tendon injury and the like; 3. the invention adopts reasonable sealing and sterile design, and the whole process of using the invention does not need to put foreign matters into a centrifuge tube, so that the sterility of the prepared blood product is better; 4. according to the invention, a double-pipeline design is used, and the PRP pipeline is finally taken as the suction pipe, and no liquid passes through the pipeline in the whole process, so that erythrocytes can be prevented from entering the final PRP to the greatest extent, interference is reduced, and manufacturing efficiency is improved.
Drawings
FIG. 1 is a schematic view of one embodiment of a sterile medical centrifuge tube according to the present invention;
fig. 2 is a top view of the opening portion of the present invention.
Detailed Description
According to the figures 1 and 2, the invention provides a sterile medical centrifuge tube, which comprises a centrifuge tube body 1, a tube cover 2 arranged at the tube orifice of the centrifuge tube body, a liquid inlet/waste liquid discharge tube 3 and a suction tube 4 arranged in the centrifuge tube body; the tube cover 2 is provided with an opening 5 and a hole cover 6 for sealing or opening the opening 5, one end of the liquid inlet/waste liquid discharge tube 3 is communicated with the opening 5, the other end of the liquid inlet/waste liquid discharge tube contacts or approaches the bottom of the centrifuge tube body 1, one end of the suction tube 4 is communicated with the opening 5, the other end of the suction tube approaches the bottom of the centrifuge tube body 1 and is slightly higher than the bottom end of the liquid inlet/waste liquid discharge tube 3, and a selection knob 7 capable of selecting the connection of the opening 5 and the liquid inlet/waste liquid discharge tube 3 or the suction tube 4 is arranged at the lower end of the opening 5 and above the tube cover 2.
Further, the opening 5 is provided at the center of the tube cover 2 so that the suction tube can suck the platelet fraction formed after centrifugation to the maximum.
The open hole 5 is divided into an inner layer and an outer layer, the outer side of the outer layer 51 is provided with threads or embedded rings, the threads or embedded rings are matched with the inner side threads or the inner side embedded rings of the hole cover 6 to form threads or embedded connection, the inner layer 52 is an injector interface, and the inner layer is embedded or in threaded connection with a common injector or a threaded injector; further, a gap of 0.7-2.5mm is reserved between the inner layer 52 and the outer layer 51, and the gap is covered by a breathable water sealing filtering membrane 54; there is a rigid connection 53 between the inner layer and the outer layer. The hole covers can be tightly connected, and as the stress of each component is huge in the centrifugation process, liquid leakage or shaking can be prevented in the centrifugation process; in addition, the ventilation part and the liquid inlet and outlet part are arranged in the same opening and covered by the same hole cover, so that the centrifuge tube is completely sealed in the centrifugation process, and the sterility of the inside of the centrifugation process is ensured.
Further, the pipe diameters of the liquid inlet/waste liquid discharge pipe 3 and the suction pipe 4 are 0.3-1.2mm, and the bottom ends are inclined planes. The smaller pipe diameter ensures that the residual quantity of red blood cells is smaller, and the liquid level control degree is better when the operator absorbs the red blood cells.
Further, the bottom end of the suction pipe 4 is 0.5-1mm higher than the bottom end of the liquid inlet/waste liquid discharge pipe 3, the suction pipe 4 and the liquid inlet/waste liquid discharge pipe 3 are symmetrically positioned at two sides of the central shaft and are mutually adjacent, can be integrally formed, and are rigidly connected by the same material in the middle. Therefore, when the platelet part is sucked through the suction tube, the platelet part can not be touched, the interference of red blood cells is reduced as much as possible, and the precision and purity of sucking PRP are ensured. In addition, the suction pipe does not pass through any liquid before finally sucking the PRP, and thus, no other components remain.
Further, densely-protruding vertical lines or longitudinally-protruding strips for increasing rotation friction are arranged on the outer side of the selection knob 7; the inside of the suction pipe is provided with a fixing protrusion which can be fixed at two positions, the two positions enable the hole cover 6 to be respectively communicated with the liquid inlet/waste liquid discharge pipe 4 and the suction pipe 3, and the two sides of the fixing protrusion are provided with fixing plates for preventing excessive rotation so as to limit the rotation range of the fixing protrusion.
Further, the bottom of the centrifuge tube body 1 is a symmetrical reverse cone.
Further, the centrifuge tube body 1 is manufactured into appliances with various specifications within the range of 5-100mL and is provided with standard graduation marks.
Further, the centrifuge tube body 1 is tightly connected with the tube cover 2, and cannot be separated. Therefore, the problem that the tube cover is separated or opened by mistake in actual operation can be avoided, and the blood is polluted by the external environment is avoided.
The subject of the invention is also a process for the preparation of platelet rich plasma by means of a sterile medical centrifuge tube as described in any of the preceding claims.
Further, the liquid inlet/waste liquid discharge pipe is positioned at the center of the centrifuge tube, is vertical and extends to the bottom end of the centrifuge tube; the suction pipe is adjacent to the liquid inlet/waste liquid discharge pipe and slightly shorter than the liquid inlet/waste liquid discharge pipe; one end of the opening can be tightly communicated with the injector, the injector is wrapped by the inner diameter, the injector is inserted in a seamless way, one end of the opening is communicated with the liquid inlet/waste liquid discharge pipe or the suction pipe, a selection knob below the opening is used for selecting whether the opening is communicated with the liquid inlet/waste liquid discharge pipe or the suction pipe, and an external spiral cover can be used for sealing the opening above the opening
In one embodiment, the preparation method uses a secondary centrifugation method to prepare the leukocyte-enriched PRP, and the specific steps include:
step 1: after the blood of a patient is subjected to centrifugal layering in a sterile medical centrifuge tube, inserting a syringe into an opening, adjusting a selection knob to a hole cover to be communicated with a liquid inlet/waste liquid discharge pipe, and sucking a bottom erythrocyte layer from the liquid inlet/waste liquid discharge pipe;
step 2: centrifuging again, inserting the injector into the opening, adjusting the selection knob to the hole cover to be communicated with the liquid inlet/waste liquid discharge pipe, and sucking out the residual red blood cell layer at the bottom layer from the liquid inlet/waste liquid discharge pipe;
step 3: after the suction is finished, the selection knob is adjusted to the hole cover to be communicated with the suction pipe, and the blood intermediate layer (rich in white blood cells and platelets) and the components below the plasma layer are sucked from the suction pipe to obtain the white blood cell-rich PRP.
In one embodiment, the preparation method uses a one-time centrifugation method to prepare the leukocyte-depleted PRP, and the specific steps include:
step 1: after the blood of a patient is centrifugally layered in a sterile medical centrifuge tube, inserting a syringe into the opening, adjusting a selection knob to a hole cover to be communicated with a liquid inlet/waste liquid discharge pipe, and sucking out a red blood cell layer and a blood middle layer (rich in white blood cells) of the bottom layer from the liquid inlet/waste liquid discharge pipe;
step 2: and after the suction is finished, adjusting the selection knob to the hole cover to be communicated with the suction pipe, and sucking components below the plasma layer from the suction pipe to obtain the lean white blood cell PRP.
In one embodiment, the preparation method uses a secondary centrifugation method to prepare the leukocyte-depleted PRP, and the specific steps include:
step 1: after the blood of a patient is centrifugally layered in a sterile medical centrifuge tube, inserting a syringe into the opening, adjusting a selection knob to a hole cover to be communicated with a liquid inlet/waste liquid discharge pipe, and sucking out a red blood cell layer and a blood middle layer (rich in white blood cells) of the bottom layer from the liquid inlet/waste liquid discharge pipe;
step 2: centrifuging again, inserting the injector into the opening, adjusting the selection knob to the hole cover to be communicated with the liquid inlet/waste liquid discharge pipe, and sucking out the residual red blood cell layer at the bottom layer from the liquid inlet/waste liquid discharge pipe;
step 3: after the sucking is finished, the selection knob is adjusted to the hole cover to be communicated with the sucking pipe, and the components below the middle layer (rich in platelets) and the plasma layer of blood are sucked from the sucking pipe to obtain the lean white blood cell PRP.
The sterile medical centrifuge tube provided by the invention can improve the convenience of PRP manufacture, can completely avoid sample pollution in the operation process, can further improve the platelet collection rate, reduce the impurity doping of red blood cells and the like, and can collect different types of PRPs according to different use modes.
The above description of the specific embodiments of the present invention has been given by way of example only, and the present invention is not limited to the above described specific embodiments. Any equivalent modifications and substitutions for the present invention will occur to those skilled in the art, and are also within the scope of the present invention. Accordingly, equivalent changes and modifications are intended to be included within the scope of the present invention without departing from the spirit and scope thereof.
Claims (7)
1. A method for preparing platelet rich plasma by means of a sterile medical centrifuge tube, comprising the steps of:
step 1: after the blood of a patient is subjected to centrifugal layering in a sterile medical centrifuge tube, inserting a syringe into an opening, adjusting a selection knob to a hole cover to be communicated with a liquid inlet/waste liquid discharge pipe, and sucking a bottom erythrocyte layer from the liquid inlet/waste liquid discharge pipe; or (b)
After the blood of a patient is subjected to centrifugal layering in a sterile medical centrifuge tube, inserting a syringe into an opening, adjusting a selection knob to a hole cover to be communicated with a liquid inlet/waste liquid discharge pipe, and sucking a red blood cell layer and a blood middle layer at the bottom layer from the liquid inlet/waste liquid discharge pipe;
step 2: centrifuging again, inserting the injector into the opening, adjusting the selection knob to the hole cover to be communicated with the liquid inlet/waste liquid discharge pipe, and sucking out the residual red blood cell layer at the bottom layer from the liquid inlet/waste liquid discharge pipe;
step 3: after the suction is finished, adjusting a selection knob to a hole cover to be communicated with a suction pipe, and sucking components below a blood middle layer and a blood plasma layer from the suction pipe to obtain the platelet-rich blood plasma;
the sterile medical centrifuge tube comprises a centrifuge tube body (1), a tube cover (2) arranged at the tube orifice of the centrifuge tube body (1), a liquid inlet/waste liquid discharge tube (3) arranged in the centrifuge tube body (1) and a suction tube (4);
the utility model discloses a centrifugal tube, including centrifuging tube body (1), centrifuging tube body (3), liquid inlet/waste liquid discharge pipe (4), be equipped with trompil (5) on tube cover (2) and carry out sealed or open handhole door (6) to it, the one end of liquid inlet/waste liquid discharge pipe (3) with trompil (5) intercommunication, the other end contact or be close the bottom of centrifuging tube body (1), the one end of suction tube (4) with trompil (5) intercommunication, the other end is close the bottom of centrifuging tube body (1) just slightly higher than the bottom of liquid inlet/waste liquid discharge pipe (3), trompil (5) lower extreme just be equipped with in tube cover (2) top can select trompil (5) with liquid inlet/waste liquid discharge pipe (3) or suction tube (4) are connected select knob (7), the bottom of suction tube (4) is higher than liquid inlet/waste liquid discharge pipe (3) bottom 0.5-1mm, suction tube (4) with liquid inlet/waste liquid discharge pipe (3) symmetry is located the axis both sides, and is adjacent each other, but integrated into one piece, the centre is by same material rigid connection.
2. The method according to claim 1, characterized in that the opening (5) is provided in the central position of the pipe cover (2) and is divided into an inner layer and an outer layer, the outer side of the outer layer is provided with threads or embedded rings, the threads or embedded rings are matched with the inner threads or the inner embedded rings of the hole cover (6) to form threads or embedded connection, the inner layer is a syringe interface, and the inner layer is embedded or in threaded connection with a common syringe or a threaded syringe.
3. The method of claim 2, wherein a 0.7-2.5mm gap is left between the inner layer and the outer layer, the gap being covered with a gas permeable water sealing filter membrane; a rigid connection exists between the inner layer and the outer layer.
4. Method according to claim 1, characterized in that the tube diameter of the liquid inlet/waste liquid outlet tube (3) and the suction tube (4) is 0.3-1.2mm, the bottom end being beveled.
5. Method according to claim 1, characterized in that the selection knob (7) is provided on the outside with densely protruding vertical threads or longitudinal protruding strips that increase the rotational friction; the inside of the liquid inlet/waste liquid outlet pipe is provided with a fixing protrusion, so that the liquid inlet/waste liquid outlet pipe can be fixed at two positions, the two positions enable the hole cover (6) to be communicated with the liquid inlet/waste liquid outlet pipe (3) and the suction pipe (4) respectively, and two sides of the fixing protrusion are provided with fixing plates for preventing excessive rotation so as to limit the rotation range of the fixing plates.
6. Method according to claim 1, characterized in that the bottom of the centrifuge tube body (1) is a symmetrical inverted cone.
7. Method according to claim 1, characterized in that the centrifuge tube body (1) is tightly connected to the tube cover (2) and cannot be separated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610597174.XA CN106215993B (en) | 2016-07-27 | 2016-07-27 | Sterile medical centrifuge tube and preparation method thereof for preparing platelet-rich plasma |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610597174.XA CN106215993B (en) | 2016-07-27 | 2016-07-27 | Sterile medical centrifuge tube and preparation method thereof for preparing platelet-rich plasma |
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| CN106215993A CN106215993A (en) | 2016-12-14 |
| CN106215993B true CN106215993B (en) | 2023-07-28 |
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|---|---|---|---|---|
| US5215102A (en) * | 1991-10-25 | 1993-06-01 | La Mina Ltd. | Capillary blood antigen testing apparatus |
| EP0930504A2 (en) * | 1998-01-15 | 1999-07-21 | MWG -Biotech AG | Device for the gripping of a lid |
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