CN1261183C - Apparatus for preparing thrombocyte enriched blood plasma and preparing method thereof - Google Patents
Apparatus for preparing thrombocyte enriched blood plasma and preparing method thereof Download PDFInfo
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- CN1261183C CN1261183C CN 200410026230 CN200410026230A CN1261183C CN 1261183 C CN1261183 C CN 1261183C CN 200410026230 CN200410026230 CN 200410026230 CN 200410026230 A CN200410026230 A CN 200410026230A CN 1261183 C CN1261183 C CN 1261183C
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Abstract
The present invention relates to a device for preparing thrombocyte enriched blood plasma and a preparation method thereof. The device for preparing thrombocyte enriched blood plasma comprises a centrifugal tube, wherein the inside of a cavity of the centrifugal tube at least is provided with a long needle head and a short needle head, and the upper port of the centrifugal tube is connected with a sealer which is respectively connected with the needle heads and an exhaust valve. The preparation method is formed by the method that blood added with an anticoagulant is injected into the centrifugal tube through the needle heads, the centrifugal tube is put into a centrifuge to carry out centrifugation, the long needle head is used for sucking and removing liquid containing red blood cells after the blood in the tube is delaminated, blood plasma containing thrombocyte is put into the centrifuge to carry out secondary centrifugation, then the thrombocyte is condensed at the bottom of the centrifugal tube, the short needle head is used for sucking and removing the most parts of the blood plasma containing thrombocyte in the tube, and the rest blood plasma containing thrombocyte in the tube is mixed with thrombocyte groups to become thrombocyte enriched blood plasma sucked out and applied through the long needle head. The device has simple structure, easy operation, low cost and accurate volume of the obtained thrombocyte enriched blood plasma, and can effectively enrich the thrombocyte.
Description
Technical field
The present invention relates to the apparatus and method of Platelet Concentrate, be particularly useful for making the device and the preparation method of blood platelet enrichment blood plasma.
Background technology
Hinder when hemorrhage when certain position tissue damaged of body, damaged part can form sludged blood, forms sludged blood and can not lack blood platelet, and it accumulates in the blutpunkte, promotes sludged blood to form in case hemostasis liquid further runs off.Meanwhile, blood platelet discharges several growth factors, platelet derived growth factor (PDGF) most importantly, and transforming growth factors (TGF-β) etc. stimulate contiguous phoirocyte growth.These phoirocytes are the pioneers that rebuild damaged tissues, promote Wound healing.
But under nature, the local hematoblastic limited amount of assembling of damage, the growth factor amount that discharges is less, so the speed of wound healing is slower.Make speeding up of wound healing, can promote wound healing by directly using growth factor in the damage part, but these growth factors mostly are gene engineering product, cost an arm and a leg, and use single growth factor to promote wound healing, its effect is undesirable.Contain blood platelet in the body blood, PC is about 100-300 * 10 in the normal human blood
9/ liter, if the blood platelet in therefore can concentrate blood and be applied to the body damaged part can effectively promote the healing of damaged tissues.
Existing Chinese utility model patent (number of patent application is 98223340) relates to the device of Platelet Concentrate, this device comprises platelet collection bag, plasma collecting bag, conduit, puncture needle, separating bowl, the blood filtration chamber, the froth breaking chamber, three-way connection, air cleaner etc., can separate pack automatically with blood plasma to blood platelet, and with the unwanted composition feedback of receptor blood donor, the apparatus structure complexity of this Platelet Concentrate, and purpose is in order to carry out hematoblastic allosome infusion, the blood coagulation of treatment disease in the blood system or promotion body, but not promote wound healing; Another United States Patent (USP) (6,398,972) also relate to the apparatus and method of Platelet Concentrate, purpose is to promote wound healing, this device is made of two cells that linked to each other by conduit, earlier the blood after the anti-freezing is injected first Room during use, make its layering by centrifugal, by conduit hematoblastic blood plasma is contained on the upper strata and pour second Room into, carry out centrifugal once more, make the bottom of platelet deposition, pour the weary hematoblastic blood plasma in upper strata into first Room by conduit then, in second Room, obtain the blood platelet that concentrates in second Room.This device shifts at two cells that link to each other with blood component by centrifugal, effective Platelet Concentrate, but apparatus structure is complicated, need special centrifugation apparatus just can carry out during use, its range of application is very restricted, in addition, owing to use the method for toppling over to realize the transfer of liquid, the final hematoblastic blood plasma volume of enrichment that obtains can't accurately be controlled, volume is crossed conference and is caused in the blood plasma hematoblastic concentration lower, and bringing waste, the too small hematoblastic blood plasma of enrichment that can make again of volume can't cover or be full of wound, affects the treatment.
At the deficiency of existing apparatus, the invention provides a kind of simple in structurely, easy to use, with low cost, accurately controlled, adopt conventional centrifuge can prepare the device of blood platelet enrichment blood plasma and using method thereof.
Summary of the invention
The purpose of this invention is to provide a kind of simple in structurely, easy to use, with low cost, accurately controlled, adopt conventional centrifuge can prepare the device of blood platelet enrichment blood plasma.
Another object of the present invention provides a kind of method for preparing blood platelet enrichment blood plasma.
The technical scheme that realizes goal of the invention is to solve like this; Mainly consist of centrifuge tube, be equipped with minute hand head and hour hand head in the chamber of centrifuge tube at least, the upper port of centrifuge tube is connected with sealer, sealer respectively with the minute hand head with the hour hand head and have airfiltering drain tap and be connected.
4, the method for preparing blood platelet enrichment blood plasma, undertaken by following step successively:
A, the blood (6) that will be added with anti-coagulants inject centrifuge tube (3) with syringe by minute hand head (1) or hour hand head (2);
B, centrifuge tube (3) was inserted centrifugal in the centrifuge (for the first time) 4～8 minutes, its centrifugal rotational speed is 800-1200 rev/min;
C, at this moment, the blood in the centrifuge tube (3) is divided into upper and lower two-layer, and its supernatant liquid is for containing blood platelet blood plasma (7), and lower floor's liquid will contain red blood cell liquid (8) with syringe by minute hand head (1) and absorb for containing red blood cell liquid (8);
D, the remaining blood platelet blood plasma (9) that contains was inserted centrifugal in the centrifuge (for the second time) 8～12 minutes once more, its centrifugal rotational speed is 3000～5000 rev/mins, and blood platelet group this moment (11) is concentrated to centrifuge tube (3), bottom;
E, absorb by the platelet-poor plasma (10) of hour hand head (2) in will managing is most of with syringe, be blood platelet enrichment blood plasma (14) behind remaining platelet-poor plasma (13) and blood platelet group (12) mixing in the pipe, also use by minute hand head (1) sucking-off with syringe.
The present invention compared with prior art, and is simple in structure, easy to use, easy and simple to handle, with low cost, adopts conventional centrifuge to carry out.In addition, carry out the transfer of liquid, can make the volume of blood platelet enrichment blood plasma of final acquisition accurately controlled owing to use syringe.
Description of drawings
Fig. 1 is main TV structure stratiform figure of the present invention;
Fig. 2 injects the stratiform figure that is added with anti-coagulants blood for centrifuge tube;
Fig. 3 is the stratiform figure behind Fig. 2 centrifugal (for the first time);
Fig. 4 contains the stratiform figure behind the red blood cell liquid for Fig. 3 absorbs;
Fig. 5 is the stratiform figure of the blood platelet group that forms at the bottom of the platelet-poor plasma that forms of Fig. 4 centrifugal (for the second time) back and the centrifuge tube;
Fig. 6 is the stratiform figure behind Fig. 5 absorbs most of platelet-poor plasma;
Fig. 7 is the stratiform figure of the blood platelet enrichment blood plasma that forms behind Fig. 6 mixing.
The specific embodiment
Accompanying drawing is specific embodiments of the invention.
Below in conjunction with accompanying drawing content of the present invention is described further;
With reference to shown in Figure 1, mainly consist of centrifuge tube (3), at least be equipped with minute hand head (1) and hour hand head (2) in the chamber of centrifuge tube (3), the upper port of centrifuge tube (3) is connected with sealer (5), sealer (5) respectively with minute hand head (1) with hour hand head (2) and have airfiltering drain tap (4) and be connected.The lower port of centrifuge tube (3) is conical.When injecting anticoagulated blood, the gas in the centrifuge tube (3) is discharged by having airfiltering drain tap (4); During sucking-off liquid, ambient atmos enters centrifuge tube by having airfiltering drain tap (4).This device uses after sterilization, can guarantee the germ-free condition that centrifuge tube (3) is interior with airfiltering drain tap.Wherein minute hand head (1) straight cutting is gone into the bottom of centrifuge tube (3), and the length of hour hand head (2) is at least 2/3 of centrifuge tube (3) length.
The preparation method is with reference to Fig. 2 to Fig. 7.
Shown in Figure 2, the blood (6) that certain volume is added with anti-coagulants injects centrifuge tube (3) with syringe by minute hand head (1) or hour hand head (2), then centrifuge tube (3) is put into carry out on the centrifuge centrifugal;
Fig. 3 passed through centrifugal (for the first time) 4～8 minutes for Fig. 2, and its centrifugal rotational speed is 800-1200 rev/min; At this moment, the blood in the centrifuge tube (3) is divided into upper and lower two-layer, and its supernatant liquid is for containing blood platelet blood plasma (7), and lower floor's liquid is for containing red blood cell liquid (8);
Shown in Figure 4, it is lower floor among Fig. 3 to be contained red blood cell liquid (8) absorb by minute hand head (1) with syringe, because the minute hand head is positioned at the pipe end, can will contains the basic all absorptions of red blood cell liquid, and learn the volume that contains red blood cell liquid of absorption by the reading of syringe.At this moment, contain blood platelet blood plasma (9) in the centrifuge tube (3) and sink to the bottom;
Shown in Figure 5, it is to carry out centrifugal (for the second time) containing blood platelet blood plasma (9) among Fig. 4, and its centrifugation time 8～12 minutes, centrifugal rotational speed are 3000～5000 rev/mins, and blood platelet group this moment (11) is positioned at the pipe end, is platelet-poor plasma (10) above;
Shown in Figure 6, it is by hour hand head (2) shown in Figure 5 containing to be lacked the most of absorption of hematoblastic blood plasma (10) with syringe, since anticoagulation that adds and absorption to contain the red blood cell liquid volume known, by controlling the volume of the platelet-poor plasma of absorbing, just can accurately control the volume of final enrichment blood platelet blood plasma.At this moment, have only in the centrifuge tube 3 a small amount of platelet-poor plasma (13) and be positioned at the pipe end blood platelet group (12);
Shown in Figure 7, it is with a small amount of platelet-poor plasma (13) among Fig. 6 and blood platelet group (12) mixing, forms blood platelet enrichment blood plasma (14), the sucking-off of blood platelet enrichment blood plasma is also used by minute hand head (1) with syringe again.Because the minute hand head is positioned at the pipe end, can and use the basic all results of blood platelet enrichment blood plasma.
Specific embodiment
Get 30 milliliters of anticoagulations of healthy people, taking a morsel, to carry out after the platelet count be 157 * 10
9/ liter, in all the other adding apparatus, the method for pressing embodiment 2 obtains 3 milliliters of the hematoblastic blood plasma of enrichment, and carrying out platelet count is 1350 * 10
9/ liter, show that blood platelet has been concentrated about 8.6 times.
Claims (3)
1, a kind of device for preparing blood platelet enrichment blood plasma, be included in the centrifuge tube (3) that is equipped with on the centrifuge commonly used, it is characterized in that: be equipped with minute hand head (1) and hour hand head (2) in the chamber of centrifuge tube (3) at least, the upper port of centrifuge tube (3) is connected with sealer (5), and sealer (5) is connected with minute hand head (1) and hour hand head (2) and the drain tap (4) that has an air filtration effect respectively.
2, the device of preparation blood platelet enrichment blood plasma according to claim 1 is characterized in that: said centrifuge tube (3) lower end is for conical.
3, a kind of method for preparing blood platelet enrichment blood plasma, undertaken by following step successively:
A, the blood (6) that will be added with anti-coagulants inject centrifuge tube (3) with syringe by minute hand head (1) or hour hand head (2);
B, centrifuge tube (3) was inserted centrifugal in the centrifuge (for the first time) 4～8 minutes, its centrifugal rotational speed is 800-1200 rev/min;
C, at this moment, the blood in the centrifuge tube (3) is divided into upper and lower two-layer, and its supernatant liquid is for containing blood platelet blood plasma (7), and lower floor's liquid will contain red blood cell liquid (8) with syringe by minute hand head (1) and absorb for containing red blood cell liquid (8);
D, the blood platelet blood plasma (9) that contains in will managing were inserted centrifugal in the centrifuge (for the second time) 8～12 minutes, and its centrifugal rotational speed is 3000～5000 rev/mins, and blood platelet group this moment (11) is concentrated to centrifuge tube (3) bottom;
E,, part big by the platelet-poor plasma (10) of hour hand head (2) in will managing with syringe are absorbed, be blood platelet enrichment blood plasma (14) behind remaining platelet-poor plasma (13) and blood platelet group (12) mixing in the pipe, also use by minute hand head (1) sucking-off with syringe.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200410026230 CN1261183C (en) | 2004-06-11 | 2004-06-11 | Apparatus for preparing thrombocyte enriched blood plasma and preparing method thereof |
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| CN 200410026230 CN1261183C (en) | 2004-06-11 | 2004-06-11 | Apparatus for preparing thrombocyte enriched blood plasma and preparing method thereof |
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| CN1261183C true CN1261183C (en) | 2006-06-28 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106076442A (en) * | 2016-06-07 | 2016-11-09 | 腾科宝迪（厦门）生物科技有限公司 | A kind of centrifuge bottle extracted for PRP multistage parallel and using method thereof |
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| MX341634B (en) * | 2010-03-18 | 2016-08-08 | Syngen Inc | System for purifying certain cell populations in blood or bone marrow by depleting others. |
| US9164079B2 (en) * | 2011-03-17 | 2015-10-20 | Greyledge Technologies Llc | Systems for autologous biological therapeutics |
| CN104383726B (en) * | 2012-07-30 | 2016-05-25 | 第五空间健康管理江苏有限公司 | The extracting method of platelet rich plasma and the platelet rich plasma of extraction |
| CN103055365B (en) * | 2013-01-22 | 2016-03-02 | 欧阳锡林 | The blood processing system of autologous storage blood |
| CN104815764A (en) * | 2015-05-07 | 2015-08-05 | 上海交通大学附属第六人民医院 | Medical centrifugal tube and method used for producing platelet rich plasma |
| WO2016195126A1 (en) * | 2015-06-01 | 2016-12-08 | 이은혜 | Blood separation and extraction apparatus for extracting platelet-rich growth factor |
| CN106215993B (en) * | 2016-07-27 | 2023-07-28 | 上海凯戎医疗科技有限公司 | Sterile medical centrifuge tube and preparation method thereof for preparing platelet-rich plasma |
| CN108871916A (en) * | 2018-07-17 | 2018-11-23 | 厦门生命互联科技有限公司 | A kind of separation method of ultrapure blood platelet |
| CN108654142A (en) * | 2018-07-31 | 2018-10-16 | 宁波爱德康生物科技有限公司 | A kind of platelet rich plasma separator and its separation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106076442A (en) * | 2016-06-07 | 2016-11-09 | 腾科宝迪（厦门）生物科技有限公司 | A kind of centrifuge bottle extracted for PRP multistage parallel and using method thereof |
| CN106076442B (en) * | 2016-06-07 | 2017-05-17 | 腾科宝迪（厦门）生物科技有限公司 | Centrifugation bottle for PRP multistage parallel extraction, and use method thereof |
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| CN1583213A (en) | 2005-02-23 |
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Address after: High tech Zone Zhang Bayi Road 710065 Shaanxi city of Xi'an province No. 1 Huixin IBC building block B room 2401 Patentee after: Xi'an Reje Biological Technology Co., Ltd. Address before: High tech Development Zone of high road Torch Hotel 710075 Shaanxi city of Xi'an province 5 Patentee before: Ruijie Biological Medical Technology Co., Ltd., Xi'an City |