CN106076442A - A kind of centrifuge bottle extracted for PRP multistage parallel and using method thereof - Google Patents
A kind of centrifuge bottle extracted for PRP multistage parallel and using method thereof Download PDFInfo
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- CN106076442A CN106076442A CN201610399268.6A CN201610399268A CN106076442A CN 106076442 A CN106076442 A CN 106076442A CN 201610399268 A CN201610399268 A CN 201610399268A CN 106076442 A CN106076442 A CN 106076442A
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- cavity
- prp
- conduit
- centrifuge bottle
- air scoop
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- External Artificial Organs (AREA)
- Centrifugal Separators (AREA)
Abstract
The present invention provides a kind of centrifuge bottle extracted for PRP multistage parallel and using method thereof, wherein centrifuge bottle is included in centrifuge bottle this most internal is provided with airtight interlayer, centrifuge bottle cavity is divided into the first cavity and second cavity of sealing by described airtight interlayer, and described first cavity and the second cavity are connected by U-tube；The first air scoop, the first conduit and blood plasma inlet it is provided with at bottle cap on described first cavity；The second air scoop and the second conduit it is provided with at bottle cap on described second cavity.The centrifuge bottle using the present invention to provide, can carry out multistage centrifugal to blood plasma, it is not necessary to transfer blood, will not be contaminated, and purity is higher.
Description
Technical field
The present embodiments relate to technical field of medical instruments, particularly to a kind of for PRP multistage parallel extract from
Heart bottle and using method thereof.
Background technology
Platelet rich plasma (Platelet Rich Plasma, be called for short PRP) be obtained by centrifugal blood containing height
The thrombocyte plasma of concentration.The platelet of human body, in addition to can providing cohesion stopping blooding when, also contains in platelet
Have much the most relevant with wound healing and bone regeneration multiple somatomedin, such as platelet derived growth factor, convert grow because of
Son, insulin like growth factor, epidermal growth factor and VEGF etc..Somatomedin in platelet,
Differentiation and propagation to cell play facilitation, and these somatomedin have cooperative response each other, promote thin with other
The factor interaction of cytoactive and impact, maintain the balance of organizational environment jointly, to wound healing, repairs and regeneration has
Important effect.Further, since PRP can extract from patient's self-blood, after extracted enrichment processes, can be used for disease of being correlated with
Sick treatment, thus abundance, draw materials conveniently, and preparation method is simple and health can be allowed to absorb.Self PRP uses and can keep away
The problem exempting from virus disseminating and immunologic rejection occurs.Therefore during PRP is widely applied to different medical domains.Such as orthopaedics, mouth
Chamber section, decorative sursery, sports medical science and aesthetic medicine.
At present, PRP preparation suit includes the centrifuge bottle for blood is centrifuged operation.But current is centrifugal
Bottle there is problems in that and is 1. easily subject to outside contamination, shifts, add platelet activation and contaminated machine in multiple containers
Meeting；2. PRP collection purity is the highest, inefficiency；3. easily being affected by operation factors, preparation method is not sufficiently stable.
Summary of the invention
For solving the problem of above-mentioned existence, the present invention provides a kind of centrifuge bottle extracted for PRP multistage parallel and makes
By method, it is possible to achieve the most quickly PRP extracts, and the PRP purity extracted is higher.
The present invention provides a kind of centrifuge bottle extracted for platelet rich plasma PRP multistage parallel, wherein: at centrifuge bottle originally
Internal being provided with airtight interlayer, centrifuge bottle cavity is divided into the first cavity and second cavity of sealing by described airtight interlayer, and described
One cavity and the second cavity are connected by U-tube；Be provided with at bottle cap on described first cavity the first air scoop, the first conduit and
Blood plasma inlet；The second air scoop and the second conduit it is provided with at bottle cap on described second cavity.
Further, the bottom of described centrifuge bottle body is coniform, described first conduit and the lower port of the second conduit
And the two-port of U-tube is positioned at described cone shape bottom.
Further, described first air scoop, the first conduit, blood plasma inlet, U-tube, the second air scoop, the second conduit
It is equipped with sealing rubber ring in bottle cap junction.
Further, it is hose connection for being positioned at described centrifuge bottle body exterior in described U-tube, is provided with switch at hose
Control device.
Further, described first air scoop, the first conduit, blood plasma inlet, the second air scoop and the second conduit from
The outside of heart bottle body is equipped with sealing lid.
Further, described interlayer is V-type, and centrifuge bottle cavity is divided into the first cavity and second cavity of sealing, described
The volume of the first cavity is more than the volume of described second cavity.
The present invention also provides for the using method of a kind of centrifuge bottle using any one PRP multistage parallel above-mentioned to extract, bag
Include:
Step one: open the first air scoop, will be injected in centrifuge bottle by blood plasma inlet containing anticoagulant fresh blood
The first cavity in, then by the first air scoop, the first conduit and blood plasma inlet seal, described centrifuge bottle is placed in centrifuge
In be centrifuged；
Step 2: after being centrifuged, opens the first air scoop, uses syringe to be extracted by the first conduit and is in the first chamber
The red blood cell layer of internal bottom, when syringe extraction to red blood cell layer is with when being in the PRP layer demarcation line lower limb in intermediate layer, stops
Only extraction, seals the first conduit mouth；
Step 3: use syringe outwards to extract the air of described second cavity by the second air scoop so that the first chamber
The PRP layer of internal bottom is acted on by air pressure, is input in described second cavity by U-tube, in being drawn into the first cavity
PRP layer and supernatant layer demarcation line top edge time, stop extraction；
Step 4: the first air scoop and the second air scoop are sealed, described centrifuge bottle is placed in a centrifuge and carries out again
Centrifugal；
Step 5: after recentrifuge completes, opens the first air scoop and the second air scoop, with syringe by the second conduit
Extract the second cavity inner bottom part red blood cell layer, when syringe extraction to red blood cell layer is with PRP layer demarcation line, exchange new injection for
Device collects the PRP layer in the second cavity by the second conduit extraction.
Further, also include between step 3 and step 4: use syringe by the first conduit by the first cavity
Interior supernatant layer extracts, and then injects containing anticoagulant fresh blood to the first cavity again by blood plasma inlet.
Further, when the PRP layer collected in centrifugal rear second cavity, can extract the most commensurability according to actual needs
The stillness of night, be mixedly configured into variable concentrations PRP.
The PRP centrifuge bottle using the present invention to provide, has the advantage that
(1) blood plasma can be carried out secondary centrifuging, and when carrying out secondary centrifuging, it is not necessary to blood is transferred to outside centrifuge bottle
Portion, directly shifts inside device, will not be contaminated；
(2) second time is to be stored in by centrifugal liquid in smaller container time centrifugal, PRP layer and the boundary of red blood cell layer
Line is obvious, easy DNA purity higher rich platelet blood；
(3) parallel multi-level can be realized extract so that same centrifuge bottle, it is achieved multistage parallel extracts, and mentions
Efficiency.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
In having technology to describe, the required accompanying drawing used is briefly described, it should be apparent that, the accompanying drawing in describing below is this
Some bright embodiments, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to
Other accompanying drawing is obtained according to these accompanying drawings.
The structural representation of the centrifuge bottle embodiment one extracted for PRP multistage parallel that Fig. 1 provides for the present invention；
Fig. 2 is the structural representation of the bottle cap embodiment one in Fig. 1；
Fig. 3 is the structural representation of the bottle cap embodiment two in Fig. 1；
The centrifuge bottle using effect figure extracted for PRP multistage parallel that Fig. 4 provides for the present invention.
Reference:
1 centrifuge bottle 2 airtight interlayer 3U type pipe
10 first cavity 11 first air scoop 12 first conduits
13 blood plasma inlet 20 second cavity 21 second air scoops
22 second conduits
Detailed description of the invention
For making the purpose of the embodiment of the present invention, technical scheme and advantage clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
The a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art
The every other embodiment obtained under not making creative work premise, broadly falls into the scope of protection of the invention.
The embodiment of the present invention provides a kind of centrifuge bottle extracted for platelet rich plasma PRP multistage parallel, such as Fig. 1～figure
Shown in 2, being provided with airtight interlayer 2 in centrifuge bottle body 1, centrifuge bottle cavity is divided into the first chamber of sealing by described airtight interlayer 2
Body 10 and the second cavity 20 (outside of two cavitys can be according to using labelling high scale), described first cavity 10 and the second cavity
20 are connected by U-tube 3；First air scoop the 11, first conduit 12 and blood plasma note it is provided with at bottle cap on described first cavity 10
Entrance 13；The second air scoop 21 and the second conduit 22 it is provided with at bottle cap on described second cavity 20.
In said structure, for the ease of the liquid bottom collecting, it is preferable that the bottom of described centrifuge bottle body 1 is circular cone
Shape, the two-port of described first conduit 12 and the lower port of the second conduit 22 and U-tube 3 is positioned at described cone shape bottom.
In said structure, in order to increase sealing, it is preferable that described first air scoop the 11, first conduit 12, blood plasma inject
Mouth 13, U-tube the 3, second air scoop the 21, second conduit 22 are equipped with sealing rubber ring in bottle cap junction.And it is described first empty
QI KOU the 11, first conduit 12, blood plasma inlet the 13, second air scoop 21 and the second conduit 22 are equal in the outside of centrifuge bottle body 1
It is provided with sealing lid.
When using said structure to extract PRP, it is embodied as step and may include that
Step one: open the first air scoop 11, will inject centrifugal by blood plasma inlet 13 containing anticoagulant fresh blood
Bottle 1 in the first cavity 10 in, then first air scoop the 11, first conduit 12 and blood plasma inlet 13 are sealed, by described from
Heart bottle 1 is placed in a centrifuge and is centrifuged；
Step 2: after being centrifuged, blood plasma can be divided into three layers, i.e. supernatant layer, PRP layer and red blood cell layer.Now, open
First air scoop 11, is used syringe to be extracted by the first conduit 12 and is in the red blood cell layer of bottom in the first cavity 10, work as note
To red blood cell layer, (purpose is to retain red blood cell layer and PRP with when being in the PRP layer demarcation line lower limb in intermediate layer in emitter extraction
Mixed liquor between Ceng), stop extraction, by the sealing of 12 mouthfuls of the first conduit；
Step 3: use syringe outwards to extract the air of described second cavity 20 by the second air scoop 21.Now
One air scoop 11 is open mode, and the second air scoop 21 is sealing state, then can exist in the first cavity 10 and the second cavity 20
Draught head, so that the PRP layer of bottom is acted on by air pressure in the first cavity 10, is input to described second chamber by U-tube 3
In body 20, (retain PRP layer and supernatant when the PRP layer being drawn in the first cavity 10 and supernatant layer demarcation line top edge
Layer boundary mixing liquid), stop extraction；
Step 4: the first air scoop 11 and the second air scoop 21 are sealed, described centrifuge bottle 1 is placed in a centrifuge and carries out
Recentrifuge；
Step 5: after recentrifuge completes, opens the first air scoop 11 and the second air scoop 21, with syringe by second
Conduit 22 extracts the second cavity 20 inner bottom part red blood cell layer, when syringe extraction to red blood cell layer is with PRP layer demarcation line, exchanges for
New syringe collects the PRP layer in the second cavity 20 by the second conduit 22 extraction.
Now, the PRP purity collected from the second cavity 20 is the highest, reason be on the one hand the second cavity 20 relative to
Overall centrifuge bottle, between scale value relatively accurate, the boundary between three layers that produces after being easier to distinguish recentrifuge, the
Two aspects pass through once centrifugal PRP liquid, recentrifuge on this basis exactly due to what the second cavity 20 was centrifuged
Operation, the purity mentioned will be higher.
For the ease of controlling U-tube 3, further, it is also possible in the specific implementation, described U-tube 3 be positioned at described from
Heart bottle body 1 is outside is hose connection, is provided with switch controlling device at hose.Switch controlling device does not do concrete limit herein
Fixed, if energy closed flexible pipe so that and U-tube 3 is not turned on.
In said method, in order to concurrent working can be realized, also include between step 3 and step 4: use syringe
By the first conduit 12, the supernatant layer in first cavity 10 is extracted, then again by blood plasma inlet 13 to the first cavity
10 inject containing anticoagulant fresh blood.Now, recentrifuge so that the first cavity 10 and the second cavity 20 the most parallel from
The heart, as shown in Figure 4, what the first cavity 10 was centrifugal is the primitive plasma for extracting to design sketch, and what the second cavity 20 was centrifugal is
PRP liquid after first time centrifugally operated.
PRP amount owing to containing in blood is less, further in order to increase the efficiency of extraction, when structure designs, and institute
Stating interlayer can be V-type, concrete as it is shown on figure 3, centrifuge bottle cavity is divided into the first cavity 10 and the second cavity 20, institute of sealing
State the volume volume more than described second cavity 20 of the first cavity 10.Such structure is so that as far as possible in the first chamber
Body 10 injects more primitive plasma so that the PRP amount of extraction is purer relatively greatly.
Additionally, when the PRP layer collected in centrifugal rear second cavity 20, can extract different amounts of according to actual needs
The stillness of night, it is mixedly configured into variable concentrations PRP.
Use the present invention provide PRP centrifuge bottle and using method, have pollution-free, purity is high, efficiency is high, extracted amount is big
Advantage.
Although the most more such as centrifuge bottle that employs, blood plasma inlet, the term such as conduit mouth, air scoop, but also
It is not excluded for using the probability of other term.Use these terms to be only used to more easily to describe and explain the basis of the present invention
Matter；It is all contrary with spirit of the present invention for being construed as any additional restriction.
Last it is noted that various embodiments above is only in order to illustrate technical scheme, it is not intended to limit；To the greatest extent
The present invention has been described in detail by pipe with reference to foregoing embodiments, it will be understood by those within the art that: it depends on
So the technical scheme described in foregoing embodiments can be modified, or the most some or all of technical characteristic is entered
Row equivalent；And these amendments or replacement, do not make the essence of appropriate technical solution depart from various embodiments of the present invention technology
The scope of scheme.
Claims (9)
1. the centrifuge bottle extracted for platelet rich plasma PRP multistage parallel, it is characterised in that: in centrifuge bottle body (1)
Inside being provided with airtight interlayer (2), centrifuge bottle cavity is divided into the first cavity (10) and second cavity of sealing by described airtight interlayer (2)
(20), described first cavity (10) is connected by U-tube (3) with the second cavity (20)；Bottle cap on described first cavity (10)
Place is provided with the first air scoop (11), the first conduit (12) and blood plasma inlet (13)；At bottle cap on described second cavity (20)
It is provided with the second air scoop (21) and the second conduit (22).
The centrifuge bottle that PRP multistage parallel the most according to claim 1 extracts, it is characterised in that: described centrifuge bottle body (1)
Bottom be coniform, the two-port of described first conduit (12) and the lower port of the second conduit (22) and U-tube (3) is positioned at
Described cone shape bottom.
The centrifuge bottle that PRP multistage parallel the most according to claim 1 extracts, it is characterised in that: described first air scoop
(11), the first conduit (12), blood plasma inlet (13), U-tube (3), the second air scoop (21), the second conduit (22) are tied at bottle cap
Sealing rubber ring it is equipped with at conjunction.
The centrifuge bottle that PRP multistage parallel the most according to claim 1 extracts, it is characterised in that: in described U-tube (3) position
Outside in described centrifuge bottle body (1) is hose connection, is provided with switch controlling device at hose.
The centrifuge bottle that PRP multistage parallel the most according to claim 1 extracts, it is characterised in that: described first air scoop
(11), the first conduit (12), blood plasma inlet (13), the second air scoop (21) and the second conduit (22) are in centrifuge bottle body (1)
Outside be equipped with sealing lid.
6. according to according to any one of Claims 1 to 5 PRP multistage parallel extract centrifuge bottle, it is characterised in that: described every
Layer is V-type, and centrifuge bottle cavity is divided into the first cavity (10) and second cavity (20) of sealing, the appearance of described first cavity (10)
The long-pending volume more than described second cavity (20).
7. use a using method for the centrifuge bottle that the PRP multistage parallel according to any one of claim 1～6 extracts, its
It is characterised by, including:
Step one: open the first air scoop (11), will inject centrifugal containing anticoagulant fresh blood by blood plasma inlet (13)
In the first cavity (10) in bottle (1), then by close to the first air scoop (11), the first conduit (12) and blood plasma inlet (13)
Envelope, is placed in a centrifuge described centrifuge bottle (1) and is centrifuged；
Step 2: after being centrifuged, opens the first air scoop (11), uses syringe to be extracted by the first conduit (12) and is in the
The red blood cell layer of one cavity (10) interior bottom, when syringe extraction to red blood cell layer be under the PRP layer demarcation line in intermediate layer
During edge, stop extraction, the first conduit (12) mouth is sealed；
Step 3: use syringe outwards to be extracted the air of described second cavity (20) by the second air scoop (21) so that the
The PRP layer of one cavity (10) interior bottom is acted on by air pressure, is input in described second cavity (20) by U-tube (3), when taking out
When getting the PRP layer in the first cavity (10) with supernatant layer demarcation line top edge, stop extraction；
Step 4: the first air scoop (11) and the second air scoop (21) are sealed, described centrifuge bottle (1) is placed in a centrifuge into
Row recentrifuge；
Step 5: after recentrifuge completes, opens the first air scoop (11) and the second air scoop (21), with syringe by second
Conduit (22) extracts the second cavity (20) inner bottom part red blood cell layer, when syringe extraction to red blood cell layer is with PRP layer demarcation line,
Exchange new syringe for and collect the PRP layer in the second cavity (20) by the second conduit (22) extraction.
The using method of the centrifuge bottle that PRP multistage parallel the most according to claim 7 extracts, it is characterised in that in step
Also include between three and step 4: use syringe the supernatant layer in the first cavity (10) to be taken out by the first conduit (12)
Remove, then inject containing anticoagulant fresh blood to the first cavity (10) again by blood plasma inlet (13).
The using method of the centrifuge bottle that PRP multistage parallel the most according to claim 7 extracts, it is characterised in that: collecting
After centrifugal during the PRP layer in the second cavity (20), the different amounts of stillness of night can be extracted, is mixedly configured into not according to actual needs
With concentration PRP.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610399268.6A CN106076442B (en) | 2016-06-07 | 2016-06-07 | Centrifugation bottle for PRP multistage parallel extraction, and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610399268.6A CN106076442B (en) | 2016-06-07 | 2016-06-07 | Centrifugation bottle for PRP multistage parallel extraction, and use method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN106076442A true CN106076442A (en) | 2016-11-09 |
| CN106076442B CN106076442B (en) | 2017-05-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610399268.6A Active CN106076442B (en) | 2016-06-07 | 2016-06-07 | Centrifugation bottle for PRP multistage parallel extraction, and use method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112934287A (en) * | 2021-02-26 | 2021-06-11 | 深圳市金灿华实业有限公司 | Centrifugal tube and PRP extraction method using same |
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| CN1583213A (en) * | 2004-06-11 | 2005-02-23 | 西安瑞捷生物医疗技术有限公司 | Apparatus for preparing thrombocyte enriched blood plasma and preparing method thereof |
| CN1658941A (en) * | 2002-04-08 | 2005-08-24 | 热起源公司 | Blood component separation method and apparatus |
| CN103974777A (en) * | 2011-11-01 | 2014-08-06 | 朴铉贞 | Container for centrifugal separation providing rapid centrifugal separation |
| CN104694382A (en) * | 2015-03-09 | 2015-06-10 | 余家昌 | Rapid nucleic acid extraction device and method |
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| CN1058551A (en) * | 1990-08-03 | 1992-02-12 | 让·吉根 | Device with blood plasma in the centrifugal separation separating whole blood |
| CN1261183A (en) * | 2000-02-21 | 2000-07-26 | 华南理工大学 | Management information automatization system and its autocontrol method and program for passenger traffic stations on highway |
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| CN1261183C (en) * | 2004-06-11 | 2006-06-28 | 西安瑞捷生物医疗技术有限公司 | Apparatus for preparing thrombocyte enriched blood plasma and preparing method thereof |
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| CN112934287A (en) * | 2021-02-26 | 2021-06-11 | 深圳市金灿华实业有限公司 | Centrifugal tube and PRP extraction method using same |
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| CN106076442B (en) | 2017-05-17 |
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Effective date of registration: 20210305 Address after: 530000 no.66-303, Gaoling Road, Nanning, Guangxi Zhuang Autonomous Region Patentee after: NANNING TECBOD BIOTECHNOLOGY Co.,Ltd. Address before: 5 / F, No.8 workshop, 2879 Dongfu Avenue, Haicang District, Xiamen City, Fujian Province, 361027 Patentee before: TECBOD (XIAMEN) BIOTECHNOLOGY Co.,Ltd. |