CN106187511A - A kind of microbial manure preventing and treating root-knot nematode and preparation method thereof - Google Patents
A kind of microbial manure preventing and treating root-knot nematode and preparation method thereof Download PDFInfo
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- CN106187511A CN106187511A CN201610571955.1A CN201610571955A CN106187511A CN 106187511 A CN106187511 A CN 106187511A CN 201610571955 A CN201610571955 A CN 201610571955A CN 106187511 A CN106187511 A CN 106187511A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C05G3/80—Soil conditioners
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Abstract
The present invention relates to a kind of microbial manure preventing and treating root-knot nematode, it is made up of following components: tea saponin 5 10 kilograms, smectite powder 20 40 kilograms, 10 50 kilograms of peat composed of rotten mosses powder, 20 40 kilograms of compost, nimbin 1.5 5 kilograms, 0.5 5 kilograms of sodium sulfate, polyglutamic acid 0.5 1 kilograms, poly-aspartate 13 kilograms, propylene glycol alginate 15 kilograms, bacteria agent 36 kilograms, sulfobutyl-beta cyclodextrin 0.5 3 kilograms, compound enzymic preparation 0.5 2 kilograms, NPE 15 kilograms, lanthanum chloride 0.5 1 kilograms, cocamido propyl betaine 0.5 1 kilograms.The present invention prevents and treats the microbial manure of root-knot nematode can effectively prevent and treat infecting and falling ill of root-knot nematode pathogenic bacteria, the most also can improve Soil structure, increases permeability, improves pH value, reach the multi-efficiency of volume increase, upgrading, environmental protection.
Description
Technical field
The invention belongs to microbial manure technical field, relate to a kind of microbial manure preventing and treating root-knot nematode and preparation thereof
Method.
Background technology
After plant infection root-knot nematode, growth retardation, leaf margin jaundice or dried-up, plant deadlock is the most dispirited, and fruit also ossifys and stops
Only growth.Can see at plant root and on side root, fibrous root, grow the knob that many differs in size, have in beading, have
Like chicken feet shape.Once moisture weight, diseased plant butt rot more can be caused.After time dry or before midday, overground part plant
There will be wilting symptom, the root-knot nematode morbidity later stage causes plant withered.
Summary of the invention
The present invention relates to a kind of microbial manure preventing and treating root-knot nematode, the microbial manure of this preventing and treating root-knot nematode can increase
Add the probiotics content in soil, suppress harmful bacteria breeding amount；Can effectively prevent and treat infecting and falling ill of root-knot nematode pathogenic bacteria, make
Robust plant, improves the disease-resistant insect resistance capacity of plant；The most also can improve Soil structure, increase permeability, improve pH value, reach
Volume increase, upgrading, the multi-efficiency of environmental protection.
Specifically, the present invention relates to a kind of microbial manure preventing and treating root-knot nematode, be made up of following components: tea saponin
5-10 kilogram, smectite powder 20-40 kilogram, 10-50 kilogram of peat composed of rotten mosses powder, 20-40 kilogram of compost, nimbin 1.5-5 kilogram,
0.5-5 kilogram of sodium sulfate, polyglutamic acid 0.5-1 kilogram, poly-aspartate 1-3 kilogram, propylene glycol alginate 1-5 kilogram, raw
Thing microbial inoculum 3-6 kilogram, Sulfobutyl ether β _ cyclodextrin 0.5-3 kilogram, compound enzymic preparation 0.5-2 kilogram, NPE
1-5 kilogram, lanthanum chloride 0.5-1 kilogram, cocamido propyl betaine 0.5-1 kilogram.
In one specific embodiment of the present invention, the microbial manure of described preventing and treating root-knot nematode is by following components system
Become: tea saponin 6 kilograms, smectite powder 30 kilograms, 25 kilograms of peat composed of rotten mosses powder, 30 kilograms of compost, nimbin 3 kilograms, sodium sulfate 3
Kilogram, polyglutamic acid 0.6 kilogram, poly-aspartate 2 kilograms, propylene glycol alginate 3 kilograms, bacteria agent 5 kilograms, sulphur butyl
-beta-schardinger dextrin-2 kilograms, compound enzymic preparation 1.5 kilograms, NPE 3 kilograms, lanthanum chloride 0.6 kilogram, coconut oleoyl amine
CAB 0.8 kilogram.
In one specific embodiment of the present invention, the microbial manure of described preventing and treating root-knot nematode is by following component
Make: tea saponin 6 kilograms, smectite powder 25 kilograms, 20 kilograms of peat composed of rotten mosses powder, 25 kilograms of compost, nimbin 2 kilograms, sulphuric acid
2 kilograms of sodium, polyglutamic acid 0.6 kilogram, poly-aspartate 1.5 kilograms, propylene glycol alginate 2 kilograms, bacteria agent 4 kilograms,
Sulfobutyl ether β _ cyclodextrin 1 kilogram, compound enzymic preparation 0.8 kilogram, NPE 2 kilograms, lanthanum chloride 0.8 kilogram,
Cocamido propyl betaine 0.6 kilogram.
In one specific embodiment of the present invention, the microbial manure of described preventing and treating root-knot nematode is by following component
Make: tea saponin 8 kilograms, smectite powder 35 kilograms, 45 kilograms of peat composed of rotten mosses powder, 35 kilograms of compost, nimbin 4 kilograms, sulphuric acid
4 kilograms of sodium, polyglutamic acid 0.8 kilogram, poly-aspartate 2 kilograms, propylene glycol alginate 4 kilograms, bacteria agent 5 kilograms, sulphur
Fourth group-beta-cyclodextrin 2 kilograms, compound enzymic preparation 2 kilograms, NPE 4 kilograms, lanthanum chloride 1 kilogram, cocos nucifera oil acyl
Amine CAB 1 kilogram.
In one specific embodiment of the present invention, the microbial manure of described preventing and treating root-knot nematode is by following component
Make: tea saponin 6 kilograms, smectite powder 30 kilograms, 45 kilograms of peat composed of rotten mosses powder, 25 kilograms of compost, nimbin 5 kilograms, sulphuric acid
0.5 kilogram of sodium, polyglutamic acid 1 kilogram, poly-aspartate 3 kilograms, propylene glycol alginate 2 kilograms, bacteria agent 5 kilograms, sulphur
Fourth group-beta-cyclodextrin 1 kilogram, compound enzymic preparation 2 kilograms, NPE 2 kilograms, lanthanum chloride 1 kilogram, cocos nucifera oil acyl
Amine CAB 0.5 kilogram.
In one specific embodiment of the present invention, described bacteria agent is Trichoderma viride, Paecilomyces lilacinus, Kazakhstan thatch wood
Mould, streptomyces microflavus and the mixture of aspergillus oryzae composition, their weight ratio is 2:1:1:2:1.
In one specific embodiment of the present invention, described compost is by bagasse 80-120 kilogram, Pericarppium arachidis hypogaeae 30-50
Kilogram, zeolite powder 30-50 kilogram, chicken manure 30-50 kilogram, probiotics 3-10 kilogram, tributyl citrate 1-3 kilogram and water
30-50 kilogram carries out compost fermentation at normal temperatures, and fermentation time 10-15 days, drying and crushing obtains.
In one specific embodiment of the present invention, described compost be by bagasse double centner, Pericarppium arachidis hypogaeae 40 kilograms,
Zeolite powder 40 kilograms, chicken manure 40 kilograms, probiotics 7 kilograms, tributyl citrate 2 kilograms and 40 kilograms of water are carried out at normal temperatures
Compost fermentation, fermentation time 12 days, drying and crushing obtains.
In one specific embodiment of the present invention, described compost is by bagasse 90 kilograms, Pericarppium arachidis hypogaeae 35 kilograms, boiling
Stone powder 35 kilograms, chicken manure 35 kilograms, probiotics 5 kilograms, tributyl citrate 1.5 kilograms and 35 kilograms of water are carried out at normal temperatures
Compost fermentation, fermentation time 10 days, drying and crushing obtains.
In one specific embodiment of the present invention, described compost be by bagasse 110 kilograms, Pericarppium arachidis hypogaeae 45 kilograms,
Zeolite powder 45 kilograms, chicken manure 45 kilograms, probiotics 8 kilograms, tributyl citrate 2 kilograms and 45 kilograms of water are carried out at normal temperatures
Compost fermentation, fermentation time 15 days, drying and crushing obtains.
In one specific embodiment of the present invention, described compost is by bagasse 80 kilograms, Pericarppium arachidis hypogaeae 50 kilograms, boiling
Stone powder 30 kilograms, chicken manure 50 kilograms, probiotics 3 kilograms, tributyl citrate 3 kilograms and 30 kilograms of water carry out heap at normal temperatures
Fertile fermentation, fermentation time 15 days, drying and crushing obtains.
In one specific embodiment of the present invention, described probiotics is the nitrogen-fixing bacteria of equivalent, lactic acid bacteria, yeast
Bacterium, the mixture of streptococcusfaecium enterococci strain.
In one specific embodiment of the present invention, described compound enzymic preparation is cellulase, laccase and xylanase,
The weight ratio of three is 3:1:1.
In one specific embodiment of the present invention, the preparation process of described smectite powder includes: by natural
The ratio mixing with weight ratio as 30-50:1 of montmorillonite powder and hexadecyltrimethylammonium chloride, is added to the water stirring 3-5h；Will
Gained montmorillonite powder filtration drying, joins in the aqueous solution containing 5-10% sodium chloride and the sodium lauryl sulphate of 3-5% and stirs
Mix 3-6h, filter, be dried；Then using the calcining of syllogic gradient, concrete calcination operation is: (a) calcines at 500 DEG C
30-60min；B () is warmed up to 800 DEG C with the heating rate of 8-12 DEG C/min, and insulation calcining 30-60min at such a temperature；
C () is warmed up to 1200 DEG C with the heating rate of 5-8 DEG C/min, and insulation calcining 30-60min at such a temperature, after calcining terminates,
Naturally cool to room temperature, obtain smectite powder.
In one specific embodiment of the present invention, the microbial manure of preventing and treating root-knot nematode is prepared as granulated
Shape.
Another aspect of the present invention relates to the preparation method preventing and treating the microbial manure of root-knot nematode, it is characterised in that include
Following steps:
(1) each component raw material is weighed by described weight proportion standby；
(2) first by load weighted tea saponin, smectite powder, poly-aspartate, Sulfobutyl ether β _ cyclodextrin, lanthanum chloride, coconut palm
Send in granulating disc or rotary drum granulator after oleamide CAB mix homogeneously, and coordinate and add appropriate steam
Pelletize, Task-size Controlling is at 120-150 mesh, and particle drying is standby；
(3) by peat composed of rotten mosses powder, compost, nimbin, sodium sulfate, polyglutamic acid, propylene glycol alginate, compound enzymic preparation, nonyl phenol
After polyoxyethylene ether mixes in batch mixer, add granule and the bacteria agent of preparation in step (2), after mix homogeneously, send into circle
In dish comminutor or rotary drum granulator, adding suitable quantity of water steam pelletize, Task-size Controlling, at 80-100 mesh, is dried, to obtain final product.
Detailed description of the invention
Embodiment 1: the preparation of a kind of microbial manure preventing and treating root-knot nematode
Dispensing is: tea saponin 6 kilograms, smectite powder 30 kilograms, 25 kilograms of peat composed of rotten mosses powder, 30 kilograms of compost, nimbin 3,000
Gram, 3 kilograms of sodium sulfate, polyglutamic acid 0.6 kilogram, poly-aspartate 2 kilograms, propylene glycol alginate 3 kilograms, bacteria agent 5
Kilogram, Sulfobutyl ether β _ cyclodextrin 2 kilograms, compound enzymic preparation 1.5 kilograms, NPE 3 kilograms, lanthanum chloride 0.6
Kilogram, cocamido propyl betaine 0.8 kilogram；
Described bacteria agent is Trichoderma viride, Paecilomyces lilacinus, Trichoderma harzianum, streptomyces microflavus and the mixing of aspergillus oryzae composition
Thing, their weight ratio is 2:1:1:2:1；
Described compost is by bagasse double centner, Pericarppium arachidis hypogaeae 40 kilograms, zeolite powder 40 kilograms, chicken manure 40 kilograms, probiotics 7
Kilogram, tributyl citrate 2 kilograms and 40 kilograms of water carry out compost fermentation at normal temperatures, fermentation time 12 days, drying and crushing obtains
Arrive；
Described probiotics is the mixture of the nitrogen-fixing bacteria of equivalent, lactic acid bacteria, yeast, streptococcusfaecium enterococci strain；
Described compound enzymic preparation is cellulase, laccase and xylanase, and the weight ratio of three is 3:1:1；
The preparation process of described smectite powder includes: by natural smectite powder and hexadecyltrimethylammonium chloride with weight
Amount mixes than the ratio for 40:1, is added to the water stirring 4h；By gained montmorillonite powder filtration drying, join containing 6% sodium chloride
With stirring 5h in the aqueous solution of the sodium lauryl sulphate of 4%, filter, be dried；Then use the calcining of syllogic gradient, specifically forge
Burning operation is: (a) carries out calcining 45min at 500 DEG C；B () is warmed up to 800 DEG C with the heating rate of 10 DEG C/min, and at this
At a temperature of insulation calcining 45min；C () is warmed up to 1200 DEG C with the heating rate of 6 DEG C/min, and be incubated calcining at such a temperature
45min, after calcining terminates, naturally cools to room temperature, obtains smectite powder；
Preparation method comprises the steps:
(1) each component raw material is weighed by described weight kilogram proportioning standby；
(2) first by load weighted tea saponin, smectite powder, poly-aspartate, Sulfobutyl ether β _ cyclodextrin, lanthanum chloride, coconut palm
Send in granulating disc or rotary drum granulator after oleamide CAB mix homogeneously, and coordinate and add appropriate steam
Pelletize, Task-size Controlling is at 135 mesh, and particle drying is standby；
(3) by peat composed of rotten mosses powder, compost, nimbin, sodium sulfate, polyglutamic acid, propylene glycol alginate, compound enzymic preparation, nonyl phenol
After polyoxyethylene ether mixes in batch mixer, add granule and the bacteria agent of preparation in step (2), after mix homogeneously, send into circle
In dish comminutor or rotary drum granulator, adding suitable quantity of water steam pelletize, Task-size Controlling, at 90 mesh, is dried, to obtain final product.
Embodiment 2 one kinds prevents and treats the preparation of the microbial manure of root-knot nematode
Dispensing is: tea saponin 6 kilograms, smectite powder 25 kilograms, 20 kilograms of peat composed of rotten mosses powder, 25 kilograms of compost, nimbin 2,000
Gram, 2 kilograms of sodium sulfate, polyglutamic acid 0.6 kilogram, poly-aspartate 1.5 kilograms, propylene glycol alginate 2 kilograms, bacteria agent
4 kilograms, Sulfobutyl ether β _ cyclodextrin 1 kilogram, compound enzymic preparation 0.8 kilogram, NPE 2 kilograms, lanthanum chloride 0.8
Kilogram, cocamido propyl betaine 0.6 kilogram；
Described bacteria agent is Trichoderma viride, Paecilomyces lilacinus, Trichoderma harzianum, streptomyces microflavus and the mixing of aspergillus oryzae composition
Thing, their weight ratio is 2:1:1:2:1；
Described compost is by bagasse 90 kilograms, Pericarppium arachidis hypogaeae 35 kilograms, zeolite powder 35 kilograms, chicken manure 35 kilograms, probiotics 5,000
Gram, tributyl citrate 1.5 kilograms and 35 kilograms of water carry out compost fermentation at normal temperatures, fermentation time 10 days, drying and crushing obtains
Arrive；
Described probiotics is the mixture of the nitrogen-fixing bacteria of equivalent, lactic acid bacteria, yeast, streptococcusfaecium enterococci strain；
Described compound enzymic preparation is cellulase, laccase and xylanase, and the weight ratio of three is 3:1:1；
The preparation process of described smectite powder includes: by natural smectite powder and hexadecyltrimethylammonium chloride with weight
Amount mixes than the ratio for 35:1, is added to the water stirring 3h；By gained montmorillonite powder filtration drying, join containing 5% sodium chloride
With stirring 3h in the aqueous solution of the sodium lauryl sulphate of 3%, filter, be dried；Then use the calcining of syllogic gradient, specifically forge
Burning operation is: (a) carries out calcining 30min at 500 DEG C；B () is warmed up to 800 DEG C with the heating rate of 8 DEG C/min, and in this temperature
Degree lower insulation calcining 30min；C () is warmed up to 1200 DEG C with the heating rate of 5 DEG C/min, and be incubated calcining at such a temperature
30min, after calcining terminates, naturally cools to room temperature, obtains smectite powder；
Preparation method comprises the steps:
(1) each component raw material is weighed by described weight kilogram proportioning standby；
(2) first by load weighted tea saponin, smectite powder, poly-aspartate, Sulfobutyl ether β _ cyclodextrin, lanthanum chloride, coconut palm
Send in granulating disc or rotary drum granulator after oleamide CAB mix homogeneously, and coordinate and add appropriate steam
Pelletize, Task-size Controlling is 120, and particle drying is standby；
(3) by peat composed of rotten mosses powder, compost, nimbin, sodium sulfate, polyglutamic acid, propylene glycol alginate, compound enzymic preparation, nonyl phenol
After polyoxyethylene ether mixes in batch mixer, add granule and the bacteria agent of preparation in step (2), after mix homogeneously, send into circle
In dish comminutor or rotary drum granulator, adding suitable quantity of water steam pelletize, Task-size Controlling, at 80 mesh, is dried, to obtain final product.
Embodiment 3 one kinds prevents and treats the preparation of the microbial manure of root-knot nematode
Dispensing is: tea saponin 8 kilograms, smectite powder 35 kilograms, 45 kilograms of peat composed of rotten mosses powder, 35 kilograms of compost, nimbin 4,000
Gram, 4 kilograms of sodium sulfate, polyglutamic acid 0.8 kilogram, poly-aspartate 2 kilograms, propylene glycol alginate 4 kilograms, bacteria agent 5
Kilogram, Sulfobutyl ether β _ cyclodextrin 2 kilograms, compound enzymic preparation 2 kilograms, NPE 4 kilograms, lanthanum chloride 1 kilogram,
Cocamido propyl betaine 1 kilogram；
Described bacteria agent is Trichoderma viride, Paecilomyces lilacinus, Trichoderma harzianum, streptomyces microflavus and the mixing of aspergillus oryzae composition
Thing, their weight ratio is 2:1:1:2:1；
Described compost is by bagasse 110 kilograms, Pericarppium arachidis hypogaeae 45 kilograms, zeolite powder 45 kilograms, chicken manure 45 kilograms, probiotics 8
Kilogram, tributyl citrate 2 kilograms and 45 kilograms of water carry out compost fermentation at normal temperatures, fermentation time 15 days, drying and crushing obtains
Arrive；
Described probiotics is the mixture of the nitrogen-fixing bacteria of equivalent, lactic acid bacteria, yeast, streptococcusfaecium enterococci strain；
Described compound enzymic preparation is cellulase, laccase and xylanase, and the weight ratio of three is 3:1:1；
The preparation process of described smectite powder includes: by natural smectite powder and hexadecyltrimethylammonium chloride with weight
Amount mixes than the ratio for 50:1, is added to the water stirring 5h；By gained montmorillonite powder filtration drying, join containing 10% chlorination
Sodium and 5% sodium lauryl sulphate aqueous solution in stir 6h, filter, be dried；Then the calcining of syllogic gradient is used, specifically
Calcination operation is: (a) carries out calcining 60min at 500 DEG C；B () is warmed up to 800 DEG C with the heating rate of 12 DEG C/min, and
Insulation calcining 60min at a temperature of Gai；C () is warmed up to 1200 DEG C with the heating rate of 8 DEG C/min, and be incubated calcining at such a temperature
60min, after calcining terminates, naturally cools to room temperature, obtains smectite powder；
Preparation method comprises the steps:
(1) each component raw material is weighed by described weight kilogram proportioning standby；
(2) first by load weighted tea saponin, smectite powder, poly-aspartate, Sulfobutyl ether β _ cyclodextrin, lanthanum chloride, coconut palm
Send in granulating disc or rotary drum granulator after oleamide CAB mix homogeneously, and coordinate and add appropriate steam
Pelletize, Task-size Controlling is at 150 mesh, and particle drying is standby；
(3) by peat composed of rotten mosses powder, compost, nimbin, sodium sulfate, polyglutamic acid, propylene glycol alginate, compound enzymic preparation, nonyl phenol
After polyoxyethylene ether mixes in batch mixer, add granule and the bacteria agent of preparation in step (2), after mix homogeneously, send into circle
In dish comminutor or rotary drum granulator, adding suitable quantity of water steam pelletize, Task-size Controlling, at 100 mesh, is dried, to obtain final product.
Comparative example 1(omits sodium sulfate)
Dispensing is: tea saponin 6 kilograms, smectite powder 30 kilograms, 25 kilograms of peat composed of rotten mosses powder, 30 kilograms of compost, nimbin 3,000
Gram, polyglutamic acid 0.6 kilogram, poly-aspartate 2 kilograms, propylene glycol alginate 3 kilograms, bacteria agent 5 kilograms, sulphur butyl-
Beta-schardinger dextrin-2 kilograms, compound enzymic preparation 1.5 kilograms, NPE 3 kilograms, lanthanum chloride 0.6 kilogram, coconut oleoyl amine
CAB 0.8 kilogram；Other are with embodiment 1.
Comparative example 2 (eliminating Sulfobutyl ether β _ cyclodextrin)
Dispensing is: tea saponin 6 kilograms, smectite powder 30 kilograms, 25 kilograms of peat composed of rotten mosses powder, 30 kilograms of compost, nimbin 3,000
Gram, 3 kilograms of sodium sulfate, polyglutamic acid 0.6 kilogram, poly-aspartate 2 kilograms, propylene glycol alginate 3 kilograms, bacteria agent 5
Kilogram, compound enzymic preparation 1.5 kilograms, NPE 3 kilograms, lanthanum chloride 0.6 kilogram, cocamido propyl betaine
0.8 kilogram；Other are with embodiment 1.
Comparative example 3(eliminates lanthanum chloride)
Dispensing is: tea saponin 6 kilograms, smectite powder 30 kilograms, 25 kilograms of peat composed of rotten mosses powder, 30 kilograms of compost, nimbin 3,000
Gram, 3 kilograms of sodium sulfate, polyglutamic acid 0.6 kilogram, poly-aspartate 2 kilograms, propylene glycol alginate 3 kilograms, bacteria agent 5
Kilogram, Sulfobutyl ether β _ cyclodextrin 2 kilograms, compound enzymic preparation 1.5 kilograms, NPE 3 kilograms, coconut oleoyl amine third
Base glycine betaine 0.8 kilogram；Other are with embodiment 1.
Comparative example 4:(eliminates Trichoderma viride)
Described bacteria agent is Paecilomyces lilacinus, Trichoderma harzianum, streptomyces microflavus and the mixture of aspergillus oryzae composition, they
Weight ratio is 1:1:2:1；Other are with embodiment 1.
Comparative example 5:(eliminates tributyl citrate)
Described compost is by bagasse double centner, Pericarppium arachidis hypogaeae 40 kilograms, zeolite powder 40 kilograms, chicken manure 40 kilograms, probiotics 7
Kilogram and 40 kilograms of water carry out compost fermentation at normal temperatures, fermentation time 12 days, drying and crushing obtains；Other are with embodiment 1.
Comparative example 6:(eliminates laccase)
Described compound enzymic preparation is cellulase and xylanase, and the weight ratio of three is 3:1；Other are with embodiment 1.
Comparative example 7:(eliminates 6% sodium chloride)
The preparation process of described smectite powder includes: by natural smectite powder and hexadecyltrimethylammonium chloride with weight
Amount mixes than the ratio for 40:1, is added to the water stirring 4h；By gained montmorillonite powder filtration drying, join containing 4% 12
The aqueous solution of alkyl sodium sulfate stirs 5h, filters, be dried；Then using the calcining of syllogic gradient, concrete calcination operation is:
A () carries out calcining 45min at 500 DEG C；B () is warmed up to 800 DEG C with the heating rate of 10 DEG C/min, and be incubated at such a temperature
Calcining 45min；C () is warmed up to 1200 DEG C with the heating rate of 6 DEG C/min, and insulation calcining 45min at such a temperature, calcining knot
Shu Hou, naturally cools to room temperature, obtains smectite powder；Other are with embodiment 1.
Comparative example 8:(eliminates the sodium lauryl sulphate of 4%)
The preparation process of described smectite powder includes: by natural smectite powder and hexadecyltrimethylammonium chloride with weight
Amount mixes than the ratio for 40:1, is added to the water stirring 4h；By gained montmorillonite powder filtration drying, join containing 6% sodium chloride
Aqueous solution stirs 5h, filters, be dried；Then using the calcining of syllogic gradient, concrete calcination operation is: (a) enters at 500 DEG C
Row calcining 45min；B () is warmed up to 800 DEG C with the heating rate of 10 DEG C/min, and insulation calcining 45min at such a temperature；(c)
It is warmed up to 1200 DEG C with the heating rate of 6 DEG C/min, and insulation calcining 45min at such a temperature, after calcining terminates, natural cooling
To room temperature, obtain smectite powder；Other are with embodiment 1.
The test example 1 microbial manure of the present invention field test to preventing and treating tomato in plastic greenhouse root-knot nematode
As the consumption of 30kg/ mu, the microbial manure prepared by embodiment of the present invention 1-3 and comparative example 1-8 is uniformly spread fertilizer over the fields
On the native face in experimental plot, and being mixed in soil with Cultivator, matched group uses common fertilizer, without any nematicide
Agent.Transplant 180 days " Invest, Then Investigate " plant root knot level indexes of tomato seedling (0 grade: without root nodule；The root system of 1 grade: 1-20% has root nodule；2
Level: the root system of 21-40% has root nodule；The root system of 3 grades: 41-60% has root nodule；The root system of 4 grades: 61-80% has root nodule；5 grades:
The root system of 81-100% has root nodule), and calculate disease index and preventive effect as follows:
Disease index (%)=(n1×1+n2×2+n3×3+n4×4+n5× 5)/(S × 5) × 100, n1-n5Represent root nodule respectively
Sick level index 1-5 level corresponding plant sum, S represents the plant sum of investigation.
Preventive effect (%)=100 (1-x/y), the disease index that x, y process respectively and compare.Result of the test is as shown in table 1.
Table 1
| Group | Disease index (%) | Preventive effect (%) |
| Comparison | 95.87 | 0 |
| Embodiment 1 | 8.62 | 91.01 |
| Embodiment 2 | 8.74 | 90.88 |
| Embodiment 3 | 8.50 | 91.13 |
| Comparative example 1 | 21.25 | 77.83 |
| Comparative example 2 | 18.71 | 80.48 |
| Comparative example 3 | 23.42 | 75.57 |
| Comparative example 4 | 18.20 | 81.02 |
| Comparative example 5 | 20.37 | 78.75 |
| Comparative example 6 | 19.58 | 79.58 |
| Comparative example 7 | 18.93 | 80.25 |
| Comparative example 8 | 22.72 | 76.30 |
The data of table 1 show, use microbial manure of the present invention has preferable preventive and therapeutic effect to root-knot nematode, with blank group
Compare, use microbial manure of the present invention and all can significantly reduce the morbidity of root-knot nematode；Meanwhile, in table 1 data it is also shown that omit
After the component of the present invention, effect will decline, and illustrates that each component of the present invention is the most collaborative.
Claims (8)
1. prevent and treat a microbial manure for root-knot nematode, be made up of following components: tea saponin 5-10 kilogram, smectite powder
20-40 kilogram, 10-50 kilogram of peat composed of rotten mosses powder, 20-40 kilogram of compost, nimbin 1.5-5 kilogram, 0.5-5 kilogram of sodium sulfate, poly-paddy
Propylhomoserin 0.5-1 kilogram, poly-aspartate 1-3 kilogram, propylene glycol alginate 1-5 kilogram, bacteria agent 3-6 kilogram, sulphur butyl
-beta-schardinger dextrin-0.5-3 kilogram, compound enzymic preparation 0.5-2 kilogram, NPE 1-5 kilogram, lanthanum chloride 0.5-1 thousand
Gram, cocamido propyl betaine 0.5-1 kilogram.
The microbial manure of preventing and treating root-knot nematode the most according to claim 1, wherein bacteria agent is Trichoderma viride, pale purple
Paecilomyces varioti, Trichoderma harzianum, streptomyces microflavus and the mixture of aspergillus oryzae composition, their weight ratio is 2:1:1:2:1.
The microbial manure of preventing and treating root-knot nematode the most according to claim 1, described compost is by bagasse 80-120 thousand
Gram, Pericarppium arachidis hypogaeae 30-50 kilogram, zeolite powder 30-50 kilogram, chicken manure 30-50 kilogram, probiotics 3-10 kilogram, citric acid three fourth
Ester 1-3 kilogram and 30-50 kilogram of water carry out compost fermentation at normal temperatures, and fermentation time 10-15 days, drying and crushing obtains.
The microbial manure of preventing and treating root-knot nematode the most according to claim 3, wherein said probiotics is equivalent
Nitrogen-fixing bacteria, lactic acid bacteria, yeast, the mixture of streptococcusfaecium enterococci strain.
The microbial manure of preventing and treating root-knot nematode the most according to claim 1, described compound enzymic preparation is cellulase, paint
Enzyme and xylanase, the weight ratio of three is 3:1:1.
The microbial manure of preventing and treating root-knot nematode the most according to claim 1, the system of wherein said smectite powder
Standby process includes: the ratio mixing with weight ratio as 30-50:1 by natural smectite powder and hexadecyltrimethylammonium chloride, adds
Enter stirring 3-5h in water；By gained montmorillonite powder filtration drying, join the dodecyl sulfur containing 5-10% sodium chloride and 3-5%
The aqueous solution of acid sodium stirs 3-6h, filters, be dried；Then using the calcining of syllogic gradient, concrete calcination operation is: (a) exists
Carry out at 500 DEG C calcining 30-60min；B () is warmed up to 800 DEG C with the heating rate of 8-12 DEG C/min, and be incubated at such a temperature
Calcining 30-60min；C () is warmed up to 1200 DEG C with the heating rate of 5-8 DEG C/min, and insulation calcining 30-at such a temperature
60min, after calcining terminates, naturally cools to room temperature, obtains smectite powder.
The microbial manure of preventing and treating root-knot nematode the most according to claim 1, is prepared into grain shape.
8. the preparation method of a kind of microbial manure preventing and treating root-knot nematode described in any one of claim 1-7, its feature exists
In, comprise the steps:
(1) each component raw material is weighed by described weight proportion standby；
(2) first by load weighted tea saponin, smectite powder, poly-aspartate, Sulfobutyl ether β _ cyclodextrin, lanthanum chloride, coconut palm
Send in granulating disc or rotary drum granulator after oleamide CAB mix homogeneously, and coordinate and add appropriate steam
Pelletize, Task-size Controlling is at 120-150 mesh, and particle drying is standby；
(3) by peat composed of rotten mosses powder, compost, nimbin, sodium sulfate, polyglutamic acid, propylene glycol alginate, compound enzymic preparation, nonyl phenol
After polyoxyethylene ether mixes in batch mixer, add granule and the bacteria agent of preparation in step (2), after mix homogeneously, send into circle
In dish comminutor or rotary drum granulator, adding suitable quantity of water steam pelletize, Task-size Controlling, at 80-100 mesh, is dried, to obtain final product.
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