CN106176893B - Application of radix crotonis argenteae and its extract - Google Patents
Application of radix crotonis argenteae and its extract Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Alternative & Traditional Medicine (AREA)
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- Pharmacology & Pharmacy (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the technical field of traditional Chinese medicines, and particularly discloses application of croton argentea or a croton argentea extract in preparation of a medicine for treating and/or preventing streptococcus suis.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicines, in particular to application of radix sophorae tonkinensis and an extract thereof.
Background
Streptococcus suis (Streptococcus suis) is a general term for epidemic diseases in pigs caused by Streptococcus equi subsp zooepidemicus, Streptococcus equi subsp, group D, E, L streptococci in Lancefield grouping, and Streptococcus suis. Clinically, the symptoms are mainly lymphadenectasis, meningitis, arthritis, septicemia and the like. The streptococcus suis is the main pathogen causing streptococcus suis, and can cause epidemic diseases such as swine meningitis and septicemia, and people can also infect the streptococcus suis through a specific transmission path. Kilpper-Balz and Schleifer J suggested a new group of S.suis in 1987. Since the disease is reported for the first time in 1958, the disease is increased in the 70 th 20 th century, the disease is more serious in the later 80 th century, and the disease is outbreak in large groups in many places and is endemic, so that great harm is caused. In the summer of 1998-1999, in parts of Jiangsu province, the swine herd has a large-area outbreak of the streptococcus suis type 2 disease, the morbidity and the mortality of the swine herd are both about 50%, and the related practitioners are caused to be seriously infected. The streptococcus suis type 2 infection events occurring in areas such as the capital yang of Sichuan province and the Nei river in 6-7 months in 2005 caused certain social influence and great economic loss, and attracted extensive attention. In recent years, pig farms in the Wuzhishan mountain of Hainan have mixed infection of streptococcicosis and other diseases, which causes certain loss. Although there are theoretically many drugs that can treat swine streptococcosis, such as penicillin, the drug resistance of pathogenic bacteria is becoming more serious with the widespread use of antibacterial drugs and the unreasonable clinical application.
Chinese patent CN105267916A discloses a veterinary drug for treating swine streptococcosis, which is a pure traditional Chinese medicine preparation prepared from 50g of astragalus root, 40g of emblic leafflower fruit, 15g of finger citron, 10g of great burdock achene, 8g of citron, 7g of sea-buckthorn, 7g of black sesame, 5g of nutmeg, 100g of houttuynia cordata, 45g of dried ginger, 20g of medlar, 15g of dandelion, 10g of plantain herb, 6g of fenugreek, 5g of rhodiola rosea, 4g of fructus evodiae, 4g of gordon euryale seed and 3g of pepper, and can effectively treat swine streptococcosis and avoid the drug resistance problem of current antibacterial drugs. However, the problem is that the existing traditional Chinese medicine products use more traditional Chinese medicine components, about 20 traditional Chinese medicine components are used in the above patents, which are disadvantageous in terms of production simplicity and cost, and the similar patents also contain CN105267797A and CN 105147972A. Therefore, there is a need to provide a simple and effective drug for the control of streptococcus suis.
Disclosure of Invention
In view of the above, the present invention aims to provide application of croton argentea and its extract, so that the croton argentea and its extract have significant efficacy in treating streptococcus suis, have high sensitivity to streptococcus suis, and can be used for preparation of related medicines.
Croton cascarlloides Raeusch, Euphorbiaceae (Euphorbiaceae) Croton L, the alias of the leaf of Croton L, is the common herb in Hainan Li. Growing in river valleys or seaside shrubs or sparse forests below 500 m in elevation. Produced in Taiwan, Fujian, Guangdong, Hainan, Guangxi and Yunnan provinces. Distributed in the islands of the glaze ball group of japan, philippines and southeast asia. Its branches and leaves can be used as medicine, has astringent taste and cool nature, and can be used for clearing pathogenic fire, removing toxic substance, killing parasites, relieving itching, astringing water and relieving diarrhea. Its root can be used for dispelling wind and strengthening tendons and bones. Is used for treating rheumatic ostealgia, acute carbuncle, pharyngalgia, and leukorrhagia. However, the application of the croton argentea root for treating the swine streptococcosis is not reported at home and abroad at present.
According to the application, the croton argentea root and the extract thereof can be used for treating and/or preventing the swine streptococcosis through research, so that the application of the croton argentea root or the extract thereof in preparing the medicament for treating and/or preventing the swine streptococcosis is provided.
Preferably, the extract is water extract, organic solvent extract or mixture of water extract and organic solvent extract of radix crotonis Pulveratum, and the organic solvent extract is more preferably ethanol extract.
Preferably, the medicament is an injection, a capsule, a tablet or a powder injection.
According to the application, the invention provides a medicament for treating and/or preventing swine streptococcosis, which comprises the silver leaf croton root or the silver leaf croton root extract. Wherein, the radix crotonis argenteae extract is preferably water extract, organic solvent extract or mixture of the two, and the organic solvent extract is more preferably ethanol extract of radix crotonis argenteae. The medicine is preferably injection, capsule, tablet or powder injection.
In addition, the invention also provides a croton argentea extract, which is a water extract, an organic solvent extract or a mixture of the water extract and the organic solvent extract of the croton argentea. Preferably, the organic solvent extract is an ethanol extract.
Meanwhile, the invention also provides an extraction method of the croton argentea extract, which is characterized in that the croton argentea is extracted by adding water or an organic solvent, and the obtained water extract, organic solvent extract or the combination of the water extract and the organic solvent extract is the croton argentea extract.
The further extraction scheme is that the croton argentea extract is obtained by soaking croton argentea in ethanol, heating for extraction, repeating extraction twice, mixing filtrates after each filtration, concentrating under reduced pressure, and recovering ethanol solution to fluid extract.
More preferably, the extraction scheme is that the croton argentea root is put into a decocting tool, 75% ethanol solution with the weight 10 times of the croton argentea root is added into the decocting tool, the solution is soaked for 30 minutes, the solution is boiled by strong fire at the temperature of 300 to 350 ℃ and then boiled by slow fire at the temperature of 150 to 170 ℃ for 30 minutes, and the liquid medicine is filtered while the solution is hot; adding 5 times of 75% ethanol solution into the residue, boiling with strong fire at 300-350 deg.C, decocting with slow fire at 150-170 deg.C for 30 min, filtering the medicinal liquid while it is hot, mixing the medicinal liquids for 2 times, concentrating under reduced pressure, and recovering ethanol solution to obtain fluid extract.
The invention takes the traditional Chinese medicine preparation in the patent CN105267916A as a contrast, and clinical observation and treatment are carried out on sick pigs with streptococcus suis by adopting the croton argentea ethanol extract extracted by the invention, and the result shows that the total effective rate and the contrast of the treatment of the croton argentea ethanol extract provided by the invention are both 100%, but the invention has more advantages in terms of the dosage. Meanwhile, the invention also detects the drug sensitivity to the streptococcus suis together with penicillin G, compound dixin and Junbizhi, and the result shows that the ethanol extract of the croton argentea has high sensitivity to the streptococcus suis, while penicillin G generates drug resistance.
According to the technical scheme, the application of the croton argentea roots and the extract thereof in preparing the medicament for treating and/or preventing the streptococcus suis is provided, the remarkable treatment effect is achieved by using a single traditional Chinese medicine component, the sensitivity to the streptococcus suis is high, and a new way is developed for preventing and treating the streptococcus suis.
Detailed Description
The invention discloses application of the radix sophorae tonkinensis and the extract thereof, and can be realized by appropriately improving process parameters by taking the contents as reference by the technical personnel in the field. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the invention has been described in terms of preferred embodiments, it will be apparent to those skilled in the art that the techniques of the invention can be implemented and applied by modifying or appropriately combining the applications described herein without departing from the spirit, scope and spirit of the invention.
The invention is further illustrated by the following examples.
Example 1: preparation of extract of Vietnamese croton root
Putting 50 kg of croton argentea root into a decocting tool, adding 10 times of 75% ethanol solution, soaking for 30 min to make croton argentea root soaked by the ethanol solution, boiling with strong fire at 300-350 deg.C, decocting with slow fire at 150-170 deg.C for 30 min, and filtering the liquid medicine while it is hot; adding 5 times of 75% ethanol solution into the residue, boiling with strong fire at 300-350 deg.C, decocting with slow fire at 150-170 deg.C for 30 min, filtering the medicinal liquid, mixing the medicinal liquids for 2 times, concentrating under reduced pressure, and recovering ethanol solution to obtain fluid extract (about 3 kg).
Example 2: animal testing
Control group 1: the patent CN105267916A provides a veterinary drug for treating swine streptococcicosis, which comprises 50g of astragalus, 40g of emblic leafflower fruit, 15g of fingered citron, 10g of great burdock achene, 8g of citron, 7g of sea-buckthorn, 7g of black sesame, 5g of nutmeg, 100g of houttuynia cordata, 45g of dried ginger, 20g of medlar, 15g of dandelion, 10g of plantain herb, 6g of fenugreek, 5g of rhodiola rosea, 4g of fructus evodiae, 4g of gordon euryale seed and 3g of pepper.
Putting the traditional Chinese medicinal materials in the weight into a decocting tool, and adding 5000 milliliters of purified water; soaking for 30 min to thoroughly moisten Chinese medicinal materials, boiling with strong fire, decocting with slow fire for 1 hr, filtering to remove residue to obtain Chinese medicinal liquid; heating and concentrating the traditional Chinese medicine liquid to 2000 ml to obtain the veterinary medicine for treating the swine streptococcosis.
Control group 2: 50g of astragalus membranaceus, 40g of phyllanthus emblica, 15g of fingered citron, 10g of burdock, 8g of citron, 7g of sea-buckthorn, 7g of black sesame and 5g of nutmeg. The preparation method is the same as that of the control group 1.
Test groups: 10g of the drug obtained by the production method of example 1 was added with 10ml of sterile purified water to prepare a drug solution having a concentration of about 1 g/ml.
Clinical data: the clinical observation and treatment are carried out on 62 sick pigs suffering from the streptococcus suis disease in Wuzhishan city, Hainan province, and the main disease symptoms are as follows: fever, decreased appetite, lameness or incapability of standing, shortness of breath, rhinorrhea, conjunctival flushing and the like, swine infectious atrophic rhinitis, swine contagious pleuropneumonia and porcine reproductive and respiratory syndrome are eliminated, and the diagnosis can be confirmed by finding gram-positive streptococcus through pathogenic microorganism detection. The sick pigs diagnosed with streptococcicosis suis were divided into 3 treatment groups, 21 pigs in the first group, 21 pigs in the second group, and 20 pigs in the third group. The first group was treated with the test group drugs; the second group was treated with the veterinary drug of control group 1; the third group was treated with the veterinary drug of control group 2.
(1) The treatment method comprises the following steps:
the medicines in the test group are directly taken by sick pigs, 1g of the weight of each kilogram of the pigs is taken in 3 times every day, and the change of symptoms is observed after the medicines are continuously taken for 3 days.
The veterinary drugs of the control groups 1 and 2 are directly drunk by sick pigs three times a day with each time of 100 ml and 200 ml, and the symptom change is observed after continuous treatment for 3 days.
(2) The evaluation standard of curative effect is as follows:
and (3) curing: the clinical symptoms such as fever, decreased appetite, limping or not standing, shortness of breath, nasal discharge, conjunctival flushing and the like completely disappear, and the functional activity returns to normal.
The method has the following advantages: the clinical symptoms such as fever, decreased appetite, lameness or incapability of standing, shortness of breath, nasal discharge, conjunctival flushing and the like are obviously improved.
And (4) invalidation: there was no improvement in clinical signs.
(3) The treatment effect is as follows:
a first group: 18 healed heads, accounting for 85%; effective 3 heads, accounting for 15%; invalid 0 head; the total effective rate is 100%.
Second group: cure 19 heads, accounting for 90%; 2 effective heads, accounting for 10%; invalid 0 head; the total effective rate is 100%.
Third group: 12 heads are cured, accounting for 60%; effective 6 heads, accounting for 30%; invalid 2 heads; the total effective rate is 90%.
The clinical tests show that the radix crotonalis argenteae has obvious curative effect on the swine streptococcosis, the cure rate is as high as 85%, the total effective rate is 100%, the effect is equivalent to that of the second group, but the traditional Chinese medicine components are far less than that of the existing product.
Example 3: in vitro drug susceptibility testing
1. Medicaments and strains
1.1 test drugs
Penicillin G (Penicilin G), compound dixin (Fortum) and Hardge bacteria in sterile water solution with the concentration of about 1mg/ml for later use;
the drug solutions obtained in the test group of example 2 of the present invention;
1.2 test strains
Separating the liver and spleen of the spontaneously dead pig, and obtaining 2 strains of streptococcus suis through physiological and biochemical experiments and pathogenicity test verification, wherein the number of the 2 strains is as follows: zlqj1 and zlqj 2.
2. Test method
A paper diffusion method is adopted. The stored purified strains were taken up at 37 days respectivelyCulturing in nutritive bouillon of 'shi' degree for 18-24 hr, diluting with aseptic normal saline to 2X 106pieces/mL, spread evenly on nutrient agar plates. Each flat plate is stuck with 4 pieces of drug sensitive paper, the distance between the two paper pieces is 25mm, the distance between the paper piece and the edge of the flat plate is 10 mm-15 mm, the center of the paper piece is lightly pressed to ensure tight adhesion, and the flat plate is cultured for 24 hours at the constant temperature of 37 ℃. The diameter of the inhibition zone is measured, and the sensitivity of the streptococcus suis to the drug sensitive paper is judged (the judgment standard is shown in table 1). And comparing the high sensitivity rate and the drug resistance rate according to the test result.
TABLE 1 criteria for drug susceptibility test
The test results are shown in Table 2.
TABLE 2 drug sensitivity test results of two strains of Streptococcus suis
As can be seen from the data shown in Table 2, 2 strains of Streptococcus suis were highly sensitive to Todaxin, Junbizhi and Croton tiglium, and had significant resistance to penicillins.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (6)
1. Application of radix crotonis Latifoliae or radix crotonis Latifoliae extract in preparing medicine for treating and/or preventing Streptococcus suis is provided.
2. The use of claim 1, wherein the croton argentea extract is an aqueous extract, an organic solvent extract, or a mixture thereof.
3. The use according to claim 2, wherein the organic solvent extract is an ethanol extract.
4. The use of claim 1, wherein the medicament is an injection, a capsule, a tablet or a powder injection.
5. A medicine for treating and/or preventing swine streptococcosis is characterized in that the active ingredient is the silver leaf croton root or silver leaf croton root extract.
6. The medicament of claim 5, wherein the croton argentea extract is an aqueous extract, an organic solvent extract, or a mixture thereof.
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| CN101732426A (en) * | 2010-02-10 | 2010-06-16 | 孙胜俊 | Formula of medicament for treating swine high fever and using method thereof |
| CN103720977A (en) * | 2012-10-12 | 2014-04-16 | 天津佳瑞兴科技发展有限公司 | Traditional Chinese medicine composition used for treating pig mixed infection |
| CN105079412A (en) * | 2014-05-13 | 2015-11-25 | 重庆美德实业集团有限公司 | Veterinary compound traditional Chinese medicine injection and preparation method thereof |
| CN104873675A (en) * | 2015-06-11 | 2015-09-02 | 山东新希望六和集团有限公司 | Pharmaceutical composition for treating swine high fever and preparation method thereof |
| CN105126091A (en) * | 2015-08-26 | 2015-12-09 | 梁庆乐 | Swine high fever prevention and treatment method |
| CN105233253B (en) * | 2015-11-11 | 2018-06-19 | 河南后羿实业集团有限公司 | A kind of compound Chinese medicinal preparation for treating swine fever hyperpyrexia disease and preparation method thereof |
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