CN106173207A - A kind of complex enzyme formula based on feeding guar meal and application thereof - Google Patents
A kind of complex enzyme formula based on feeding guar meal and application thereof Download PDFInfo
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- CN106173207A CN106173207A CN201610565998.9A CN201610565998A CN106173207A CN 106173207 A CN106173207 A CN 106173207A CN 201610565998 A CN201610565998 A CN 201610565998A CN 106173207 A CN106173207 A CN 106173207A
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Abstract
The present invention discloses a kind of complex enzyme formula based on feeding guar meal and application thereof, belongs to enzyme preparation application.This formula includes acid mannase 5～10%, neutral mannase 7～15%, acid protease 10～20%, neutral protease 30～60%, alkaline protease 3～10%, α tilactase 10～20%.This compound enzyme improves protein digestibility in guar meal, decomposes galactomannan in guar meal, reduces its stickiness of chyme in digestive tract, improves the digestibility of various nutritional labelings in feedstuff greatly；Reduce or eliminate the antinutritional factor in guar meal, improve the immunocompetence of animal, decreasing the waste of feedstuff, improving the utilization rate of feedstuff；Especially this complex enzyme formula can make guar meal as the expensive protein raw materials of high protein raw material substitution, can be directly added in the big production of feedstuff, reduces the production cost of feedstuff, saves feed resource, improves feed factory and the productivity effect of aquaculture.
Description
Technical field
The invention belongs to enzyme preparation application, particularly to a kind of complex enzyme formula based on feeding guar meal and
Application.
Background technology
Guar meal is the side-product after Guar beans extracts locust beam gum, and nutritive value is the highest.Guar meal contains abundant
Protein and carbohydrate, be the pure natural not genetically modified product that do not contains any chemical substance and preservative.Its albumen
Matter content is 48.9%～55.16%, and its contained aminoacid is institute in zein, wheat protein and rice protein simultaneously
Lack.Owing to guar meal is nutritious, often using it as protein supplement, use with other feed formulation.Additionally Guar
Bean cake also has the effect of dietary fiber effect and functional oligose.But, guar meal is also to there is the biggest nutrition to lack
Falling into, containing higher trypsin ihhibitor in guar meal, and can remain guar gum in guar meal, it mainly becomes
Dividing is galactomannan, due to water solublity and the stickiness of galactomannan height, can greatly increase in digestive tract and eat
Rotten stickiness, can reduce the digestibility of various nutritional labelings in feedstuff；Therefore guar meal is not as novel protein raw material
Can directly apply in feedstuff.In order to solve above auxotrophy problem, having less research the most both at home and abroad is all to use
Microbe fermentation method solves, but this method is the longest, and technology controlling and process is complicated.The most really solve guar meal to fill in feedstuff
Dividing and utilize, finding a kind of effective approach becomes crucial.
Summary of the invention
In order to overcome the shortcoming of prior art with not enough, it is an object of the invention to provide a kind of based on feeding guar meal
Complex enzyme formula.To solve the auxotrophy of existing feeding guar meal, improve feed digestion utilization rate.This compound enzyme both may be used
To be used for producing feeding guar meal raw material in vitro, the more important Feed Manufacturing that can also be directly used in, the most effectively
Remove the antitrypsin inhibitive factor in guar meal, galactomannan of degrading, reduces its viscosity.Two kinds of application approaches
All can be effectively improved the rate that digests and absorbs of animal, improve animal survival rate, increase feed factory and the productivity effect of aquaculture.
Another object of the present invention is to provide the application of above-mentioned complex enzyme formula based on feeding guar meal.
The purpose of the present invention is achieved through the following technical solutions:
A kind of complex enzyme formula based on feeding guar meal, including following component by mass percentage:
Acid mannase 5～10%, neutral mannase 7～15%, acid protease 10～20%, neutral egg
White enzyme 30～60%, alkaline protease 3～10%, alpha-galactosidase 10～20%.
Described acid mannonase neutrality mannonase acid protease, neutral protease, alkaline protease
With the mass percent of alpha-galactosidase and be 100%.
The vigor of described enzyme is respectively: acid mannase 30000～50000U/g, neutral mannase 30000
～50000U/g, acid protease 50000～100000U/g, neutral protease 50000～100000U/g, alkaline protease
50000～100000U/g, alpha-galactosidase 1000～2000U/g.
The application in producing feeding guar meal feedstuff of the described complex enzyme formula based on feeding guar meal.
Apply above-mentioned compound enzyme to produce one of feeding guar meal feedstuff method, can follow these steps to carry out:
With the guar meal that pulverizes and sieves as raw material；
Weigh 0.1～0.2% compound enzyme of material quantity, soluble in water；
Add guar meal, by material-water ratio 2:(3～5) mixing, pH is adjusted to 4.5～7.0, is warming up to 37～40 DEG C；
At the uniform velocity agitation reaction 4～6 hours, is then neutralized, is dried.
Described sieves as crossing 20～40 mesh sieves；
Described material-water ratio is preferably 2:(3～4)；
Described pH is adjusted to 4.5～7.0 preferably with hydrochloric acid or NaOH, pH is adjusted to 4.5～7.0.
Described is adjusted to preferably 4.5～6.5 by pH；
Application compound enzyme produces the two of feeding guar meal feedstuff method, can follow these steps to carry out: with guar meal be
Protein raw materials deployment and conjugation feedstuff, addition 0.01～the compound enzyme of 0.02% forage volume in its mixed feed, by mixed feed
Production technology mix homogeneously, Direct-fed.
The basic theories basis of the present invention is: utilizes the nutritive peculiarity of guar meal, in conjunction with animal gastrointestinal tract feature, seeks
Under animal digestion physiological condition can not only efficient degradation albumen, improve raw material digestibility, but also can eliminate or be passivated
Trypsin ihhibitor in guar meal, and the complex enzyme formula of galactomannan of can degrading, thus it is former to reach this
Material both can be directly added in Feed Manufacturing, can fully absorb again the purpose of utilization in vivo.Production process is simple, reduces feedstuff
Production cost, and enzymatic degradation process may also provide more small-molecular peptides and saccharide, reduces amino acid whose loss, thus improves
Nutrient efficiency, promotes the health of animal.
The present invention, relative to prior art, has such advantages as and effect:
One is to improve protein digestibility in guar meal, decomposes galactomannan in guar meal, reduces it and disappearing
Change the stickiness of chyme in road, improve the digestibility of various nutritional labelings in feedstuff greatly；Two is to reduce or eliminate Guar
Antinutritional factor in bean cake, improves the immunocompetence of animal, decreases the waste of feedstuff, improves the utilization rate of feedstuff；Three
Guar meal as high protein raw material can the expensive protein raw materials of fictitious hosts, can be directly added in the big production of feedstuff,
Reduce the production cost of feedstuff, save feed resource, improve feed factory and the productivity effect of aquaculture.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Acid mannonase neutrality mannonase acid protease used by embodiment, neutral protease, alkali
Property protease, alpha-galactosidase are all commercially to be commercially available.
Described guar meal, for India's import guar meal.
Embodiment 1
Apply above-mentioned compound enzyme to produce one of feeding guar meal feedstuff method, can follow these steps to carry out:
With pulverized 40 mesh sieves guar meal as raw material；
Preparation compound enzyme: accurately weigh acid mannase (50000U/g) 50g, neutral mannase (50000U/
G) 70g, acid protease (100000U/g) 100g, neutral protease (50000U/g) 600g, alkaline protease (50000U/g)
50g, alpha-galactosidase (1500U/g) 130g, mix homogeneously.
Weigh 1000kg guar meal, pulverized 40 mesh sieves, the most load weighted above-mentioned compound enzyme is added in 2000kg water
Stir, add guar meal, after mix homogeneously, adjust pH4.5 with hydrochloric acid, be warming up to 37 DEG C；After reacting 6 hours, carry out
Neutralize, be dried.Test is carried out at certain large-scale hoggery, and the outer ternary ablactational baby pig that 21 ages in days, health, body weight are close is chosen in test
120, gilt and galt half and half, average body weighs about 6.3 ± 0.3kg, and the principle consistent with body weight by sex is randomly divided into 3
Individual process, each process 4 repetition, each repetition one hurdle, 10, each hurdle pig.
Test group is that the protein raw materials substituted in formula with the guar meal after above-mentioned enzymolysis prepares compound feed for piglets,
Matched group is the mixed feed (this feed formula reference standard NRC2012) of the protein raw materials bean cake preparation not substituted.Two weeks
Result is (as shown in table 1) later:
The result of the test that Production Performance of Weaning Pigs and diarrhea rate are affected by test group is shown in Table 2.As shown in Table 2, test group
The average daily gain of piglet is significantly higher than matched group (P ＜ 0.05), improves 17.37% than matched group, feedstuff-meat ratio test group
Feedstuff-meat ratio reduces 12.10% (P ＜ 0.05) than matched group, and test group piglet diarrhea rate is substantially less than matched group (P ＜
0.05), have dropped 76.0% than matched group.
The guar meal that table 1 processes through the compound enzyme impact on weaned piglets
| Project | Matched group | Test group |
| Initial weight, kg | 6.35±0.67 | 6.25±0.72 |
| End weight, kg | 8.25±0.92 | 8.48±0.98 |
| Daily gain, g | 190±16a | 223±24b |
| Daily ingestion amount, g | 236±20 | 243±26 |
| Feedstuff-meat ratio | 1.24±0.10a | 1.09±0.12b |
| Diarrhea rate, % | 6.25±1.66a | 1.50±0.38b |
Note: colleague's data shoulder indicates same letter and represents that difference is not notable (P > 0.05), represents that difference shows without same letter
Write (P < 0.05).
Embodiment 2
Apply above-mentioned compound enzyme to produce one of feeding guar meal feedstuff method, can follow these steps to carry out:
With pulverized 40 mesh sieves guar meal as raw material；
Preparation compound enzyme: accurately weigh acid mannase (40000U/g) 140g, neutral mannase
(30000U/g) 300g, acid protease (70000U/g) 300g, neutral protease (80000U/g) 1000g, alkaline protease
(70000U/g) 60g, alpha-galactosidase (2000U/g) 200g, mix homogeneously.Weigh 1000kg guar meal, pulverize 20
Mesh sieve, adds the most load weighted above-mentioned compound enzyme in 1500kg water and stirs, add guar meal, after mix homogeneously,
Adjust pH 6.5 with hydrochloric acid, be warming up to 40 DEG C；After reacting 4 hours, it is neutralized, is dried.Test is entered large-scale broiler field in Guangdong
OK, test is chosen 20,000 yellow-feather broilers and is tested,
Test group is to substitute the daily ration of protein raw materials in daily ration of broiler with the guar meal after above-mentioned enzymolysis, and matched group is not
The protein raw materials daily ration (this feed formula reference standard NRC1994) substituted.Off-test after raising 56 days, result of the test is
(as shown in table 2): test group, compared with matched group, is significantly higher than matched group in terms of daily gain, and other indexs are without significant difference.
The guar meal that table 2 processes through the compound enzyme impact on yellow-feather broiler production performance
| Project | Matched group | Test group |
| Deliver weight, kg for sale | 2.97±0.12a | 2.99±0.05a |
| Survival rate, % | 98.87±1.88a | 98.98±2.11a |
| Feedstuff-meat ratio | 2.34±0.05a | 2.33±0.08a |
| Daily gain, g | 43.48±1.55a | 44.92±1.87b |
Note: colleague's data shoulder indicates same letter and represents that difference is not notable (P > 0.05), represents that difference shows without same letter
Write (P < 0.05).
Embodiment 3
Produce one of feeding guar meal feedstuff method based on above-mentioned compound enzyme further to work out this compound enzyme
Directly apply in the mixed feed with guar meal as protein raw materials, the production application of more convenient feed factory.Both applied this multiple
Synthase produces the two of feeding guar meal feedstuff method, can follow these steps to carry out:
Prepare compound enzyme according to the following ratio: acid mannase (30000U/g) 10%, neutral mannase
(40000U/g) 10%, acid protease (50000U/g) 20%, neutral protease (100000U/g) 30%, alkaline protease
(30000U/g) 10%, alpha-galactosidase (1000U/g) 20%, mix homogeneously.Weigh 1000kg guar meal to substitute and join
Close protein raw materials in feedstuff, mixed feed adds 0.2kg compound enzyme, after mix homogeneously, Direct-fed body according to feedstuff per ton
The ablactational baby pig of weight about 11kg.Matched group is former basal diet (this feed formula reference standard NRC2012).Result such as table 3
Shown in, the average daily gain of test group piglet is significantly higher than matched group (P ＜ 0.05), improves 18.8% than matched group, expects meat
Reducing 14% (P ＜ 0.05) than test group than matched group, test group piglet diarrhea rate is substantially less than matched group (P ＜
0.05), have dropped 76.4% than matched group.
Table 3 is directly added into the impact on weaned piglets of the compound enzyme guar meal feedstuff
| Project | Matched group | Test group |
| Initial weight, kg | 11.1±0.36 | 11.0±0.05 |
| End weight, kg | 20.81±0.64 | 21.76±0.10 |
| Daily gain, g | 325.08±16.12a | 386.26±6.42b |
| Daily ingestion amount, g | 690±0.01 | 730±0.04 |
| Feedstuff-meat ratio | 2.14±0.11a | 1.84±0.10b |
| Diarrhea rate, % | 15.22±3.63a | 3.59±2.65b |
Note: colleague's data shoulder indicates same letter and represents that difference is not notable (P > 0.05), represents that difference shows without same letter
Write (P < 0.05).
Embodiment 4
Apply this compound enzyme to produce the two of feeding guar meal feedstuff method, can follow these steps to carry out:
Prepare compound enzyme according to the following ratio: acid mannase (30000U/g) 7%, neutral mannase
(50000U/g) 15%, acid protease (100000U/g) 10%, neutral protease (80000U/g) 50%, alkaline protease
(80000U/g) 8%, alpha-galactosidase (1000U/g) 10%, mix homogeneously.Weigh 1000kg guar meal to replace in broiler
Protein raw materials preparation broiler fodder, add the above-mentioned compound enzyme prepared, it is multiple that addition is that broiler fodder per ton adds 0.1kg
Synthase.Matched group is the broiler fodder (this feed formula reference standard NRC (1994)) not substituted.Test large-scale in Guangdong
Broiler field is carried out, and 20,000 yellow-feather broilers are chosen in test, and off-test after raising 56 days, result of the test is as shown in table 2: test group
Compared with matched group, index is significantly higher than matched group in every respect, illustrates to make Guar beans by this complex enzyme formula effect
The dregs of rice directly apply to Feed Manufacturing as protein raw materials such as the existing bean cake of novel protein raw material substitution.
Table 4 is directly added into the impact on meat chicken production performance of the compound enzyme guar meal feedstuff
| Project | Matched group | Test group |
| Deliver weight, kg for sale | 2.44±0.09a | 2.52±0.05b |
| Survival rate, % | 92.35±2.94a | 93.66±3.70b |
| Feedstuff-meat ratio | 1.80±0.09a | 1.77±0.14b |
| Daily gain, g | 57.98±2.17a | 59.71±1.50b |
Note: colleague's data shoulder indicates same letter and represents that difference is not notable (P > 0.05), represents that difference shows without same letter
Write (P < 0.05).
Embodiment 5
Apply this compound enzyme to produce the two of feeding guar meal feedstuff method, can follow these steps to carry out:
Prepare compound enzyme according to the following ratio: acid mannase (30000U/g) 5%, neutral mannase
(50000U/g) 7%, acid protease (50000U/g) 10%, neutral protease (100000U/g) 60%, alkaline protease
(100000U/g) 5%, alpha-galactosidase (1500U/g) 13%, mix homogeneously.
Weighing 1000kg guar meal and replace fish flour in shrimp material, add the above-mentioned compound enzyme prepared, addition is per ton
Shrimp material adds this compound enzyme of 0.15kg, produces shrimp material.Matched group is shrimp material formula (this feed formula reference standard not substituted
NRC2011).Carrying out culture of Penaeus vannamei test, test carries out 30 days.Result of the test is shown in Table 5, the weightening finish of test group shrimp
Effect is obvious, and feedstuff prawn survival rate of the present invention, rate of body weight gain are significantly higher than matched group group (P ＜ 0.05), and feed coefficient is than comparison
Group reduces 11.3%, and this is probably complex enzyme formula and improves the shrimp utilization rate to nutrient substance such as protein in feedstuff, from
And promote the growth of prawn.
Table 5 is directly added into compound enzyme guar meal feedstuff prawn growth effect
| Index | Matched group | Test group |
| Average survival (%) | 91.5 | 93.2 |
| Average growth rate (%) | 30.1 | 36.5 |
| Than rate of body weight gain (%) | 100 | 121.2 |
| Feed coefficient | 1.23 | 1.09 |
The present invention is that feed industry, aquaculture provide a kind of safe and reliable, higher new method of benefit, has bigger
Application value.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not by above-described embodiment
Limit, the change made under other any spirit without departing from the present invention and principle, modify, substitute, combine, simplify,
All should be the substitute mode of equivalence, within being included in protection scope of the present invention.
Claims (9)
1. a complex enzyme formula based on feeding guar meal, it is characterised in that include following component the most by mass percentage:
Acid mannase 5～10%, neutral mannase 7～15%, acid protease 10～20%, neutral protease
30～60%, alkaline protease 3～10%, alpha-galactosidase 10～20%；
Described acid mannonase neutrality mannonase acid protease, neutral protease, alkaline protease and α-
The mass percent of tilactase and be 100%.
Complex enzyme formula based on feeding guar meal the most according to claim 1, it is characterised in that: the vigor of described enzyme
Respectively: acid mannase 30000～50000U/g, neutral mannase 30000～50000U/g, acid protease
50000～100000U/g, neutral protease 50000～100000U/g, alkaline protease 50000～100000U/g, α-gala
Glycosidase 1000～2000U/g.
3. the complex enzyme formula based on feeding guar meal described in claim 1 or 2 is in producing feeding guar meal feedstuff
Application.
Application the most according to claim 3, it is characterised in that: application compound enzyme produce feeding guar meal feedstuff method it
One, follow these steps to carry out:
With the guar meal that pulverizes and sieves as raw material；
Weigh 0.1～0.2% compound enzyme of material quantity, soluble in water；
Add guar meal, by material-water ratio 2:(3～5) mixing, pH is adjusted to 4.5～7.0, is warming up to 37～40 DEG C；At the uniform velocity
Agitation reaction 4～6 hours, is then neutralized, is dried.
Application the most according to claim 4, it is characterised in that:
Described sieves as crossing 20～40 mesh sieves.
Application the most according to claim 4, it is characterised in that:
Described material-water ratio is 2:(3～4).
Application the most according to claim 4, it is characterised in that:
It is described that pH is adjusted to 4.5～7.0 is, with hydrochloric acid or NaOH, pH is adjusted to 4.5～7.0.
Application the most according to claim 4, it is characterised in that:
Described being adjusted to by pH is 4.5～6.5.
Application the most according to claim 3, it is characterised in that: application compound enzyme produce feeding guar meal feedstuff method it
Two, follow these steps to carry out: with guar meal for protein raw materials deployment and conjugation feedstuff, in its mixed feed add 0.01～
The compound enzyme of 0.02% forage volume, by technique of producing mixed feed mix homogeneously, Direct-fed.
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Cited By (6)
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| CN110093333A (en) * | 2019-04-25 | 2019-08-06 | 天津挑战博德生物技术有限公司 | A kind of application in compound protease and its low-protein diet |
| CN110438190A (en) * | 2019-09-12 | 2019-11-12 | 北京瓜尔润科技股份有限公司 | A kind of method and Guar active peptide using a variety of enzyme complex enzyme hydrolysis preparation Guar active peptide |
| CN111466485A (en) * | 2020-05-22 | 2020-07-31 | 济南百斯杰生物工程有限公司 | Application of α -galactosidase in enzymatic hydrolysis of soybean meal |
| CN113528420A (en) * | 2021-07-28 | 2021-10-22 | 四川润格生物科技有限公司 | Yeast hydrolysate and preparation method and application thereof |
| CN115005014A (en) * | 2022-07-20 | 2022-09-06 | 湖南果秀食品有限公司 | Preparation method of edible fungus culture medium containing guar meal |
| CN115322927A (en) * | 2022-07-29 | 2022-11-11 | 武汉新华扬生物股份有限公司 | Compound biological agent and application thereof in guar meal feed |
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| CN110438190A (en) * | 2019-09-12 | 2019-11-12 | 北京瓜尔润科技股份有限公司 | A kind of method and Guar active peptide using a variety of enzyme complex enzyme hydrolysis preparation Guar active peptide |
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| CN113528420A (en) * | 2021-07-28 | 2021-10-22 | 四川润格生物科技有限公司 | Yeast hydrolysate and preparation method and application thereof |
| CN115005014A (en) * | 2022-07-20 | 2022-09-06 | 湖南果秀食品有限公司 | Preparation method of edible fungus culture medium containing guar meal |
| CN115322927A (en) * | 2022-07-29 | 2022-11-11 | 武汉新华扬生物股份有限公司 | Compound biological agent and application thereof in guar meal feed |
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Application publication date: 20161207 |
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