CN104830828A - Specific compound enzyme for husk barley and full-husk barley-type feed and preparation method thereof - Google Patents
Specific compound enzyme for husk barley and full-husk barley-type feed and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a specific compound enzyme for husk barley and a full-husk barley-type feed and a preparation method thereof. The compound enzyme includes beta-glucanase, arabinoxylanase, cellulase, phytase, neutral protease, alkali protease and pectinase. The full-husk barley-type feed includes the ingredients of full-husk barley, whole-fat extruded soybean, soybean meal, cottonseed protein, corn protein, soybean oil, calcium hydrogen phosphate, stone flour, lysine, methionine, threonine, sodium chloride, sodium bicarbonate, and a premix containing the above compound enzyme and a pigment. By adding the specific compound enzyme for husk barley, the anti-nutritional factors in the full-husk is eliminated, and combination of a variety of materials solves the problem of imbalance between energy value and arginine of the husk barley. The husk barley can be uses as the main feed ingredient to completely replace high quality chicken feed such as corn, and is very applicable to the high-quality chickens resistant to crude feed in large and middle stages.
Description
Technical field
The invention belongs to poultry feed technical field, be specifically related to barley special composite enzyme and full barley type feed and preparation method thereof.
Background technology
Along with the fast development of China's livestock industry, the feed resource of China supplies nervous, and the import total amount of China's dregs of beans in 2014, more than 6,000 ten thousand tons, goes back import a large amount of corn, Chinese sorghum homenergic feed simultaneously at present every year.Current corn, value of the meal are in a high position always.Develop new feed resource to be conducive to reducing costs, meet the needs of China's aquaculture sustainable and healthy development.At present, the high-quality chicken amount of delivering for sale of China accounts for the half that total amount delivered for sale by China's broiler chicken.High-quality chicken is low relative to the feed efficiency of white meat-type chickens, and vegetative period is long, therefore the feed total amount that high-quality chicken consumes every year is far longer than the feed that white meat-type chickens consumes.
In the current situation, the cost ratio corn of barley is low 200 ~ 400 yuan/ton, its feeding value is equivalent to 95% of corn, starch content is a little less than corn, crude protein is higher than corn by nearly more than 30%, and from amino acid composition, Methionin is almost 2 times of corn, methionine(Met) is a little more than corn, and to grow closely-related Nicotinic Acid Content higher than corn more than 2 times with poultry growth.In addition, the binding property of barley is good, uses barley that the granularity of finished product material can be made good.The consumption of barley adds the use that conference reduces corn, dregs of beans etc.
Patent of invention " a kind of barley chicken feed and preparation method thereof " (application number: 201310372358.2) mention barley is used for chicken feed, this feed is by barley 480 ~ 500, soya-bean cake 100 ~ 120, alfalfa hay 8 ~ 10, green hay 8 ~ 10, willow leaf 7 ~ 9, condensed milk 90 ~ 100, corncob powder 90 ~ 100, sorghum flour 90 ~ 100, Semen Crataegi powder 20 ~ 25, white sugar 35 ~ 40, red date powder 35 ~ 40, phagostimulant 4 ~ 5 are made, and effect is better.But in this patent of invention barley main and soya-bean cake, condensed milk, alfalfa hay, corn cob meal etc. with the use of, that does not consider barley can the relatively low problem of value.In addition, what use in the present invention is barley, and the usage quantity in broiler fodder is more than 50%.Patent of invention " production method of barley fermented feed " (application number: 201210501899.6) feeding dorking after barley fermentation is achieved good effect, but need repeatedly boiling, fermentation in preparation process, complex steps, operational difficulty when measuring large.
Direct barley complete replacement of corn feeding dorking often there will be certain negative impact, as food consumption reduces, and speed of growth reduction etc.
Summary of the invention
For above-mentioned deficiency of the prior art, the invention provides a kind of barley special composite enzyme; Present invention also offers a kind of full barley type feed; In addition, present invention also offers the preparation method of this full barley type feed.
A kind of barley special composite enzyme, comprises beta-glucanase 3000 ~ 5000u/g, arabinoxylanase 20000 ~ 40000u/g, cellulase 500 ~ 1500u/g, phytase 8000 ~ 15000u/g, neutral protease 400 ~ 800u/g, Sumizyme MP 600 ~ 1000u/g and polygalacturonase 500 ~ 800u/g.Above-mentioned enzyme all directly can buy commercial goods.
Barley contains a large amount of antinutritional factor, as beta-glucan, araboxylan, phytate phosphorus etc.In barley, non-starch polysaccharide content is high, glucan content about 3.3%, is 33 times of corn; Araboxylan about 7.6%, it is 2.3 times of corn, beta-glucan accounts for 75% of barley cell walls, it has higher Coefficient shrinkage, in feed, the use of barley can increase the viscosity of chyme greatly, reduce endogenous enzyme to the digestibility and utilization of barley Middle nutrition material, finally cause the digestive utilization ratio of barley Middle nutrition material to reduce.Same araboxylan also can increase the viscosity of alimentary canal content.After adding a large amount of barleys in feed, 1. the Negotiation speed of chyme reduces, and can reduce the food consumption of livestock and poultry.High viscosity can make the amount of drinking water of poultry increase simultaneously, and the nutrient of excretion also can increase; 2. the ability of enteron aisle mechanically mixing content declines.High viscosity can make each component mixing in chyme uneven.In chyme, nutrient accumulation in enteron aisle can make pathogenic micro-organism amount reproduction, causes maldigestion etc.; 3. Water soluble pentosan is not only difficult to be utilized by poultry but also viscosity by increasing intestinal contents, stops the digestibility and utilization of other nutrient particularly fat and energy.
β ~ dextranase in barley special composite enzyme, the Main Function of arabinoxylanase are the impacts eliminating β ~ dextran, araboxylan in barley, are directly degraded to the carbohydrate that poultry can utilize.To consider in barley that high, the amino acid whose utilization ratio of content of cellulose is on the low side relative to corn, containing certain phytate phosphorus, therefore have in this prozyme and add certain cellulase, neutral protease, Sumizyme MP and phytase mating reaction to overcome the above problems.Beta-glucanase, arabinoxylanase, cellulase, phytase, neutral protease, Sumizyme MP and polygalacturonase are coordinated to use in above-mentioned amount ranges, the impact of antinutritional factor in barley can be eliminated well, improve the character of barley, increase its raising property, improve nutritive value.
Preferably, above-mentioned barley special composite enzyme, beta-glucanase 4000u/g, arabinoxylanase 20000u/g, cellulase 400u/g, phytase 8000u/g, neutral protease 500u/g, Sumizyme MP 1000u/g and polygalacturonase 800u/g.
Above-mentioned barley special composite enzyme is preparing the application in feed also in protection scope of the present invention.
A kind of full barley type feed, raw material comprises: barley, full-fat extruded soybean, dregs of beans, cottonseed protein, zein, soybean oil, secondary calcium phosphate, stone flour, Methionin, methionine(Met), Threonine, sodium-chlor, sodium bicarbonate, Preblend and pigment;
Described Preblend contains: vitamin A, vitamins D, vitamin-E, vitamin K, VitB1, riboflavin, pantothenic acid, nicotinic acid, pyridoxol, vitamin H, folic acid, vitamin B12, choline, copper, iron, manganese, zinc, selenium, iodine and above-mentioned barley special composite enzyme.
Being worth lower than corn of barley, if replace corn completely with barley, it can be worth the nutritional need that can not meet high-quality chicken, therefore coordinate in feed and higher full-fat extruded soybean, Zein powder and the soybean oil of value can solve this problem, add the arginine of a small amount of cottonseed protein energy balanced feed.The crude fiber content of barley is also higher than corn, but high-quality chicken crude feed tolerance, certain fibre content contributes to the gastral growth of high-quality chicken and health.Work in coordination based on above each composition, barley can be applied in large chicken stage feed by replacement of corn completely in high-quality chicken, greatly reduce feed formulation cost.Also in meeting, the nutritional need of large chicken stage high-quality chicken normal growth, ensures that its intestinal growth is normal and healthy.Consider that in barley, pigment content is low, also add certain pigment.
Preferably, above-mentioned full barley type feed, the consumption of each composition of described Preblend is:
In Preblend, each composition is mainly for meeting the normal need of animal to trace element, VITAMIN, coordinates barley special composite enzyme together, eliminates the impact of antinutritional factor better.
Preferably, above-mentioned full barley type feed, each Composition Weight Parts:
Preferably, above-mentioned full barley type feed, described soybean oil is level Four soybean oil, and described pigment is natural pigment.Described natural pigment is plant milk extract, such as preferred Flos Tagetis Erectae extract.
Preferably, above-mentioned full barley type feed, each Composition Weight Parts:
The preparation method of above-mentioned full barley type feed, step comprises:
(1) the macrobead composition in raw material is ground into the Powdered of below particle diameter 2.5mm, adds all the other material compositions except soybean oil, mixing and stirring;
(2) powder raw material that step (1) mixes carries out steam regulation of texture, conditioning of texture, reaches the object of slaking;
(3) feedstock transportation after step (2) slaking being entered ring mould compression ratio is that the nodulizer of 1:9 is granulated; carry out after pelletizing forming contraflow cooler being cooled to room temperature; obtain finished granule, in pelletization, by rear spraying equipment, soybean oil is sprayed to feed surface.
Preferably, the preparation method of above-mentioned full barley type feed, the condition of step (2) described steam regulation of texture, conditioning of texture is vapor pressure is 0.4 MPa, and raw aqueous rate is 17%, and refining temperature is 82 DEG C, and conditioning period is 25 seconds.
Above-mentioned full barley type feed is mainly used in yellow-feathered broiler of feeding, especially in the large chicken stage.
The present invention has following beneficial effect:
The present invention's full barley type feed, with the high-quality chicken feed of barley as the complete replacement of corn of main feedstuff raw material etc., adds barley special composite enzyme simultaneously, eliminates the impact of antinutritional factor in barley; Feed formulation can be worth the uses such as higher full-fat extruded soybean, Zein powder, and what overcome barley can the relatively low defect of value, simultaneously by adding cottonseed protein balance arginine.Consider that in barley, pigment content is low, except adding Zein powder, also add certain natural pigment (Flos Tagetis Erectae extract).Finally reach the object of complete replacement of corn, greatly reduce feed cost, increase economic efficiency.
Because the robust fibre of barley is relatively high, high-quality chicken crude feed tolerance, therefore full barley type feed of the present invention, be applicable to large chicken stage use in high-quality chicken more.
Embodiment
The composition measurement of embodiment 1 barley
Table 1 barley composition measurement result
| Project | Measured value |
| Dry-matter, % | 87.0～90.0 |
| Crude protein, % | 10.0～13.0 |
| Robust fibre, % | 4.60～5.00 |
| Crude fat, % | 1.50～1.90 |
| Calcium, % | 0.08～0.10 |
| Phosphorus, % | 0.31～0.35 |
| Phosphorus can be utilized, % | 0.15～0.17 |
| Chicken Apparent metabolizable energy, kcal/kg | 2800～2900 |
| The main essential amino acids content of poultry | |
| Threonine, % | 0.40～0.43 |
| Gelucystine, % | 0.18～0.21 |
| Methionine(Met), % | 0.16～0.20 |
| Methionin, % | 0.40～0.45 |
| Tryptophane, % | 0.10～0.14 |
| Arginine, % | 0.60～0.65 |
| The Average True digestibility of main indispensable amino acid, % | 80.0 |
| Beta-glucan, % | 2.8～3.6 |
| Araboxylan, % | 5.0～8.0 |
| Nicotinic acid, mg/kg | 35～60 |
As can be seen from the index of analyzing and testing, barley crude protein content, higher than corn, can utilize phosphorus content low, to the True Amino Acid Digestibility of poultry relatively low (about 80.0%), lower than corn more than 5%.In addition, to the Apparent metabolizable energy value of poultry lower than corn.Antinutritional factor beta-glucan and arabinoxylan content higher.
Embodiment 2
Produce 1 ton of yellow-feather broiler fodder formula as following table 2:
The full barley type feed formulation of table 2
Wherein contain barley special composite enzyme (250g/ ton) in Preblend, wherein beta-glucanase 4000u/g, arabinoxylanase 20000u/g, cellulase 400u/g, phytase 8000u/g, neutral protease 500u/g, Sumizyme MP 1000u/g, polygalacturonase 800u/g.
The preparation method of described full barley type feed completes according to following processing step:
A, by after the raw material such as barley, dregs of beans, Zein powder of each for table 2 weight removing impurity, carry out being crushed to Powdered respectively with the pulverizer of 2.5mm sieve.
B, then the raw material after pulverizing is put into after mixing machine carries out mixing and stirring, the Methionin of described parts by weight, methionine(Met), Threonine, sodium bicarbonate, salt, Preblend, stone flour, secondary calcium phosphate, barley special composite enzyme are put in the lump by mixing machine small powder opening for feed after secondary premix, then remix is even together for mixed raw material powder.
C, the powder raw material mixed obtained is delivered into quality-adjusting device carry out steam regulation of texture, conditioning of texture, reach the object of slaking, the vapor pressure in modulator process is 0.4 MPa, and raw aqueous rate is 17%, and refining temperature is 82 DEG C, and conditioning period is 25 seconds.
D, the feedstock transportation after obtained modulation to be entered ring mould compression ratio be that the nodulizer of 1:9 is granulated; carry out after pelletizing forming contraflow cooler being cooled to room temperature; obtain finished granule, in pelletization, by rear spraying equipment, level Four soybean oil is sprayed to feed surface.
E, sampling experiment room after the assay was approved, become 40kg/ bag by the packaging bags with inner membrance, feeding for yellow-feathered broiler.
Embodiment 3
Test diet recipe is as following table 3:
Table 3
Wherein control group yellow-feathered broiler is identical with the trophic level of the corresponding diet of test group yellow-feathered broiler, identical feed of feeding for 1 ~ 21 day, and its body weight average is 420g, from 22 ages in days formally test.
Produce feed according to the feed manufacturing program in above diet recipe and example 2, then carry out high-quality chicken feeding experiment, test point three treatment group, each process 6 repetition, each repetition 75 chickens.Below test and carry out in summer, have no and use barley to have negative impact under the condition of heat stress.
Control group: feed A1 in 22 ~ 43 days, feeds A2 in 43 ~ 63 days;
Barley not enzyme-added group: 22 ~ 42 days feeds B1, within 43 ~ 63 days, feeds B2;
Barley feeds C1 in enzyme-added group: 22 ~ 42 days, within 43 ~ 63 days, feeds C2.
Feed add barley on 22 ~ 63 age in days high-quality chicken growth performances to affect result as shown in table 4 below, average daily food consumption between each group and between surviving rate all without significant difference (P>0.23), but the enzyme-added rear surviving rate of barley improves, the Average weight increasing a day that barley is enzyme-added group remarkable (P < 0.01) is higher than corn group, feed consumption weightening finish ratio is significantly lower than (P < 0.01) corn group, and the growth performance of the not enzyme-added group broiler chicken of barley is lower than corn group and enzyme-added group of barley, this may be relevant with antinutritional factor such as the dextran of high-content in barley and araboxylans.Simultaneously completely replacement of corn use a chicken feed cost average specific control group of barley group low 0.34 yuan/only, namely feed cost is about low than control group by about about 3.0%.
Table 4 feed adds barley to the impact of 22 ~ 63 age in days high-quality chicken growth performances
Note: same column letter is different represents that between average, difference extremely significantly (P<0.01).
The high-quality chicken feed that the present invention obtains in Guangdong, Guangxi, the ground such as Anhui carries out a large amount of administering transgenic, result shows that effect is better compared with conventional corn ~ bean pulp type diet, and feed cost is per ton lower about 100 ~ 150 yuan than conventional corn ~ bean pulp type feed.Yellow-feathered broiler large-scale soon feed of the present invention 22 ~ 63 days total augment weights of feeding reach 1.4 ~ 1.7 kilograms, feed consumption weightening finish is than about 2.55 ~ 2.70, colourity reaches the requirement of client, and it is better than corn group to find to use the digestic property of barley enzyme-added group of broiler chicken, may improve relevant with the fiber of feed relative to corn group.
Claims (10)
1. a barley special composite enzyme, it is characterized in that, comprise β ~ dextranase 3000 ~ 5000 u/g, arabinoxylanase 20000 ~ 40000 u/g, cellulase 500 ~ 1500 u/g, phytase 8000 ~ 15000 u/g, neutral protease 400 ~ 800 u/g, Sumizyme MP 600 ~ 1000 u/g and polygalacturonase 500 ~ 800 u/g.
2. barley special composite enzyme according to claim 1, it is characterized in that, β ~ dextranase 4000 u/g, arabinoxylanase 20000u/g, cellulase 400 u/g, phytase 8000u/g, neutral protease 500 u/g, Sumizyme MP 1000 u/g and polygalacturonase 800 u/g.
3. the barley special composite enzyme described in claim 1 or 2 is preparing the application in feed.
4. a full barley type feed, it is characterized in that, raw material comprises: barley, full-fat extruded soybean, dregs of beans, cottonseed protein, zein, soybean oil, secondary calcium phosphate, stone flour, Methionin, methionine(Met), Threonine, sodium-chlor, sodium bicarbonate, Preblend and pigment;
Described Preblend contains: the barley special composite enzyme described in vitamin A, vitamins D, vitamin-E, vitamin K, VitB1, riboflavin, pantothenic acid, nicotinic acid, pyridoxol, vitamin H, folic acid, vitamin B12, choline, copper, iron, manganese, zinc, selenium, iodine and claim 1 or 2.
5. full barley type feed according to claim 4, it is characterized in that, the consumption of each composition of described Preblend is:
Vitamin A 2500 ~ 6000 IU/kg;
Vitamins D 800 ~ 2000 IU/kg;
Vitamin-E 10 ~ 60 IU/kg;
Vitamin K 0.3 ~ 0.8 mg/kg;
VitB1 1.0 ~ 3.0 mg/kg;
Riboflavin Tetrabutyrate .0 ~ 5.0 mg/kg;
Pantothenic acid 6 ~ 20 mg/kg;
Nicotinic acid 20 ~ 50 mg/kg;
Pyridoxol 2.0 ~ 6.0 mg/kg;
Vitamin H 0.10 ~ 0.30 mg/kg;
Folic acid 0.40 ~ 0.80 mg/kg;
Vitamin B12 0.01 ~ 0.02 mg/kg;
Choline 500 ~ 1200 mg/kg;
Copper 8 ~ 10 mg/kg;
Iron 60 ~ 100 mg/kg;
Manganese 80 ~ 120 mg/kg;
Zinc 40 ~ 120 mg/kg;
Selenium 0.15 ~ 0.30 mg/kg;
Iodine 0.30 ~ 0.80 mg/kg;
Barley special composite enzyme 0.200 ~ 0.250 g/kg.
6. the full barley type feed according to claim 4 or 5, is characterized in that, each Composition Weight Parts:
Barley 500 ~ 800 parts;
Full-fat extruded soybean 10 ~ 100 parts;
Dregs of beans 10 ~ 150 parts;
Cottonseed protein 10 ~ 100 parts;
Zein 10 ~ 100 parts;
Soybean oil 10 ~ 60 parts;
Secondary calcium phosphate 10 ~ 18 parts;
Stone flour 8 ~ 15 parts;
Methionin 1 ~ 10 part;
Methionine(Met) 0.1 ~ 8 part;
Threonine 0.1 ~ 6 part;
1 ~ 3 part, sodium-chlor;
Sodium bicarbonate 1 ~ 3 part;
Preblend 5 ~ 15 parts;
Pigment 1 ~ 3 part.
7. full barley type feed according to claim 6, it is characterized in that, described soybean oil is level Four soybean oil, and described pigment is natural pigment.
8. full barley type feed according to claim 7, is characterized in that, each Composition Weight Parts:
Barley 680 parts;
Full-fat extruded soybean 65 parts;
Dregs of beans 20 parts
Cottonseed protein 69 parts;
Zein 60 parts;
Level Four soybean oil 60 parts;
Secondary calcium phosphate 10.2 parts;
Stone flour 9.2 parts;
Methionin 8.0 parts;
Methionine(Met) 1.8 parts;
Threonine 1.6 parts;
2.2 parts, sodium-chlor;
Sodium bicarbonate 2.0 parts;
Preblend 10 parts;
Natural pigment 1 part.
9. the preparation method of the arbitrary described full barley type feed of claim 4 ~ 8, it is characterized in that, step comprises:
(1) the macrobead composition in raw material is ground into the Powdered of particle diameter 2.5 below mm, adds all the other material compositions except soybean oil, mixing and stirring;
(2) powder raw material that step (1) mixes carries out steam regulation of texture, conditioning of texture, reaches the object of slaking;
(3) feedstock transportation after step (2) slaking being entered ring mould compression ratio is that the nodulizer of 1:9 is granulated; carry out after pelletizing forming contraflow cooler being cooled to room temperature; obtain finished granule, in pelletization, by rear spraying equipment, soybean oil is sprayed to feed surface.
10. the preparation method of full barley type feed according to claim 6, is characterized in that, the condition of step (2) described steam regulation of texture, conditioning of texture is vapor pressure is 0.4 MPa, and raw aqueous rate is 17%, and refining temperature is 82 DEG C, and conditioning period is 25 seconds.
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| CN105104859A (en) * | 2015-08-28 | 2015-12-02 | 青岛大信饲料有限公司 | Low-mycotoxin clean sow compound feed |
| CN105831443A (en) * | 2016-03-28 | 2016-08-10 | 上海新农饲料股份有限公司 | High-oil barley granule compound feed with low pulverization ratio for pig and a preparation method thereof. |
| CN105941938A (en) * | 2016-06-15 | 2016-09-21 | 环山集团有限公司 | Pure barley pregnant sow feed and production method thereof |
| CN109456956A (en) * | 2018-11-06 | 2019-03-12 | 济南百斯杰生物工程有限公司 | Beer process barley by-product enzymolysis processing enzymatic compositions and processing method |
| CN110140827A (en) * | 2019-05-28 | 2019-08-20 | 四川铁骑力士实业有限公司 | A kind of biological feedstuff and complete diet pellet of precious jade chicken |
| CN110558444A (en) * | 2019-08-09 | 2019-12-13 | 江门市得宝集团有限公司 | Quail egg mixed feed capable of improving pattern and hardness of quail egg shells |
| CN112544788A (en) * | 2020-12-09 | 2021-03-26 | 新疆希普生物科技股份有限公司 | Cottonseed protein-containing nutritional composition and preparation method and application thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105104859A (en) * | 2015-08-28 | 2015-12-02 | 青岛大信饲料有限公司 | Low-mycotoxin clean sow compound feed |
| CN105831443A (en) * | 2016-03-28 | 2016-08-10 | 上海新农饲料股份有限公司 | High-oil barley granule compound feed with low pulverization ratio for pig and a preparation method thereof. |
| CN105941938A (en) * | 2016-06-15 | 2016-09-21 | 环山集团有限公司 | Pure barley pregnant sow feed and production method thereof |
| CN109456956A (en) * | 2018-11-06 | 2019-03-12 | 济南百斯杰生物工程有限公司 | Beer process barley by-product enzymolysis processing enzymatic compositions and processing method |
| CN109456956B (en) * | 2018-11-06 | 2022-03-18 | 济南百斯杰生物工程有限公司 | Enzyme composition for enzymolysis treatment of barley by-product in beer processing process and treatment method |
| CN110140827A (en) * | 2019-05-28 | 2019-08-20 | 四川铁骑力士实业有限公司 | A kind of biological feedstuff and complete diet pellet of precious jade chicken |
| CN110558444A (en) * | 2019-08-09 | 2019-12-13 | 江门市得宝集团有限公司 | Quail egg mixed feed capable of improving pattern and hardness of quail egg shells |
| CN112544788A (en) * | 2020-12-09 | 2021-03-26 | 新疆希普生物科技股份有限公司 | Cottonseed protein-containing nutritional composition and preparation method and application thereof |
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