CN101133779A - Highly effective biological zyme micro-zoology composite feed additive - Google Patents
Highly effective biological zyme micro-zoology composite feed additive Download PDFInfo
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Abstract
The present invention relates to a high-effective bioenzyme microecological composite feed additive. The invented feed additive uses NSP enzyme as main enzyme system and uses acid proteinase and cellulose as auxiliary enzyme system, and is matched with functional oligose microecological preparation with antibiotic function. Said additive contains the following components: beta-mannase, xylanase, acid proteinase, cellulose, mannoligose and oligoxylose.
Description
Technical field
The present invention relates to a kind of composite feed additive, relate in particular to the highly effective biological zyme micro-zoology feed addictive of a kind of NSP of containing enzyme system and function oligosaccharides probiotics.
Technical background
Along with the progress of biotechnology, biological enzyme formulation has obtained extensive use in feed additive field, includes single enzyme preparation and complex enzyme formulation kind.Wherein, mannase, zytase fodder enzyme preparation occupy the bigger market share.On the other hand, be that the function oligosaccharides feeding micro-ecological preparation of representative also is applied on feed addictive market with manna oligosacchride, wood oligose.
Disclosed achievement in research shows that biology enzyme has its obvious specificity characteristics as a kind of biocatalyst efficiently, and different substrates needs different enzymes and comes catalytic decomposition.Widely used in China mainly is dregs of rice class feeds such as dregs of beans, cotton dregs, the dish dregs of rice, contains SNSPs (NSP) such as a large amount of xylans, mannosan in these feeds.These NSP are referred to as ANFs in animal nutrition, not only be difficult to by digestibility and utilization, also cause in the animal and bird intestines chyme viscosity too high easily, have reduced the utilization rate of feed.If with the degraded of physics or chemical method or remove it, difficulty is big and cost is high, and removes with corresponding biological enzyme formulation, is most economical and effective way.Mannase, wood oligose enzyme all belong to the NSP enzyme, has common characteristic: 1. strengthen Oilgosaccharkdes effect in the body effectively, promote decomposition, the digestion of mannosan, wood oligose etc. in the dregs of rice class feed, improve the micro-ecological environment of animal intestinal, improve the energy i (in vivo) metaboilic level; 2. eliminate of the harmful effect of NSP ANFs, reduce chyme viscosity, strengthen the useful effect of endogenous enzymes, increase the absorption of duodenum, improve the nutrient utilization of feed amino acid and glucose to digesting and assimilating; 3. promote secretion of growth hormone, increase lean meat percentage and lean tissue area, strengthen Abwehrkraft des Koepers; 4. reduce the transition immune response of livestock and poultry, improve livestock birds health and improve the Production of Livestock and Poultry ability.
Economic and the high feed of efficient of feeding is the key that aquaculture is produced.From the angle of economy and nutrition, the development of feed technology objectively requires feed resource more extensive, and this requires the digestion power of livestock and poultry to have with it to mate competence exertion well to act on accordingly.But true really not so, the digestion adaptive capacity of livestock and poultry is always not enough.At this problem, people have developed feeding biological complex enzyme, according to the digestive physiological characteristics of livestock and poultry, with the method for adding exogenous enzymes, the diet feed of separate sources are deployed into the high-efficiency feed that the suitable livestock and poultry of basic unification digest and assimilate.
In the existing enzyme preparation, be widely used in the feed biology enzyme and mainly contain: galactosidase, effectively decompose the alpha-galactose-glycoside key flatulence factor that contains in the grouts feed, improve the efficiency of feed utilization of dregs of beans; Acid protease, the digestion that is used to improve forage protein; Cellulase, the native cellulose in can hydrolysate feed.
Disclosed achievement in research also shows, the function oligosaccharides has outstanding biological effectiveness: 1. have the Oilgosaccharkdes effect, optimize intestinal microecology system, effects such as growth regulating of participation Bifidobacterium and adhesion, suppress growing of the assorted bacterium of enteron aisle, the host is played helpful effect; 2. discern pathogen, hinder the adhesion of pathogen and intestinal mucosa,, will get rid of external by its non-digestibility; 3. have certain immunogenicity, can stimulate immune response, strengthen the phagocytic activity of macrophage.The function oligosaccharides has obtained the extensive approval of feed industry as antibiotic substitute.
As everyone knows, the antibiotic of feeding for a long time can cause that bacterial drug resistance increases, and causes side effects such as animal food antibiotic residue easily, and health and food security are constituted a threat to.Public community is progressively taking drastic measure, bans use of antibiotic as feed addictive, and is also more and more stricter to the requirement of antibiotic residues in animal-derived food simultaneously.Eliminating antibiotic residue has become the ten minutes urgent realistic that the feed processing industry faces, and is the significant problem that is related to Business survival.
On the present domestic and international market, feeding biology enzyme additive is separate product with the feeding micro-ecological additive with antibiotic alternative functions, and effect is single, costs an arm and a leg.Its market share is essentially minority foreign brand name product and monopolizes.But, in the market also not with feeding biological enzyme formulation and feeding micro-ecological preparation characteristics, Cheap highly effective, noresidue safety-type composite feed additive product.
Summary of the invention
Purpose of the present invention is exactly in order to overcome the defective that above-mentioned prior art exists, provide a kind of with feeding biological enzyme formulation and feeding micro-ecological preparation characteristics, can eliminate the NSP ANFs in the dregs of rice class feed and substitute the biological zyme micro-zoology composite feed additive of antibiotic, highly effective and safe, noresidue.
Purpose of the present invention is achieved through the following technical solutions: with the NSP enzyme is that main enzyme is, acid protease, cellulase are auxiliary enzymes system, are equipped with the function oligosaccharides probiotics with antibiotic effect.NSP enzyme of the present invention includes mannase, zytase, galactosidase; The function oligosaccharides probiotics that is adopted is manna oligosacchride or wood oligose.
Among the present invention, various components in proportions are as follows: beta-mannase enzymatic activity 3000～60000IU/mL, xylanase activity 2000～100000IU/mL, galactosidase activity 100～10000IU/mL, acid protease activity 100～1000IU/mL, cellulase activity 50～300IU/mL, and add the wood oligose of the manna oligosacchride or 0.01～0.8% (w/w) of 0.01～0.8% (w/w) on this basis.Particularly adopt following optimization component ratio: beta-mannase enzymatic activity 10000IU/mL, xylanase activity 60000IU/mL, galactosidase activity 300IU/mL, acid protease activity 400IU/mL, cellulase activity 50～300IU/mL and manna oligosacchride 0.03% (w/w) or wood oligose 0.07% (w/w).
Biology enzyme of the present invention and function oligosaccharides can be liquid preparations, also can be solid pharmaceutical preparations.
Compare with existing product, the present invention has the following advantages:
1. the characteristics that have feeding biological enzyme formulation: exogenous enzymes such as unexistent acid protease in the animal body, cellulase can be provided, add in the feed, significantly short digestion, absorption all be arranged for the livestock and poultry of all ages and classes.
2. have the feeding micro-ecological preparation characteristics: by the effect of NSP enzyme system, decompose the NSP in the dregs of rice class feed, eliminate its ANFs effect, improve energy absorption and the Nutrition and Metabolism level of feed.
3. has the characteristic that meets biology enzyme: the biology enzyme that contains more than one simultaneously, changed the single drawback of biological in the past exogenous enzymes, can make full use of the synergy of enzyme, significantly improve the nutritive value and the utilization rate of feed, make that the scope of application of feedstuff is more extensive.
4. substitute antibiotic: feeding micro-ecological preparation in the past is to use single NSP enzyme, utilizes enzyme degraded NSP to produce oligosaccharides.This must keep higher activity after just requiring the NSP enzyme to enter body, could produce antibiotic substitution effect.And the activity of enzyme often is achieved by a variety of factors.The present invention directly adds the function oligosaccharides, with NSP enzyme system synergy, can bring into play the Oilgosaccharkdes effect of function oligosaccharides rapidly, plays the effect of antibiotic substitute.
Embodiment
Below in conjunction with embodiment, the invention will be further described.The specific descriptions of embodiment do not constitute any limitation scope of the present invention.
The preparation of embodiment 1 composite feed additive
A kind of composite feed additive, it comprises following composition: beta-mannase enzymatic activity 10000IU/mL, xylanase activity 60000IU/mL, galactosidase activity 300IU/mL, acid protease activity 400IU/mL, cellulase activity 100IU/mL and manna oligosacchride 0.03% (w/w).Its preparation technology is as follows:
Get the liquid preparation of 'beta '-mannase, zytase, galactosidase, acid protease, cellulase, respectively through enzyme biopsy survey, dosing, composite after, obtain complex enzyme formulation, standby.Get the manna oligosacchride solid pharmaceutical preparation again, after meeting purified water allotment, carry out compositely according to the final ratio of 0.03% (w/w) and above-mentioned complex enzyme formulation, obtain the composite feed additive of composition proportion as mentioned above.
The composite feed additive that adopts said method to obtain can reach following index:
The solid type liquid-type
The tiny powder of organoleptic indicator's yellowish-brown does not have the caking brown liquid
Sanitary index Escherichia coli solid enzyme is less than 30CUF/mL
Salmonella is negative
Plumbous smaller or equal to 30mg/kg
Inorganic arsenic is smaller or equal to 10mg/kg
The appropriate pH of product of the present invention is 4.0～8.0, and optimal pH is 6.0.Preference temperature is 15 ℃～60 ℃.Under 70 ℃, 5min condition, the active fall of complex enzyme formulation is applicable to the moment prilling in the feed granulating process less than 20%.
The preparation of embodiment 2 composite feed additives
A kind of composite feed additive, it comprises following composition: beta-mannase enzymatic activity 10000IU/mL, xylanase activity 60000IU/mL, galactosidase activity 300IU/mL, acid protease activity 400IU/mL, cellulase activity 100IU/mL and wood oligose 0.07% (w/w).Its preparation technology is as follows:
Get the liquid preparation of 'beta '-mannase, zytase, galactosidase, acid protease, cellulase, respectively through enzyme biopsy survey, dosing, composite after, obtain complex enzyme formulation, standby.Get the wood oligose liquid preparation again, carry out compositely according to the final ratio of 0.07% (w/w) and above-mentioned complex enzyme formulation, obtain the composite feed additive of composition proportion as mentioned above.
The preparation of embodiment 3 composite feed additives
A kind of composite feed additive, it comprises following composition: beta-mannase enzymatic activity 8000IU/mL, xylanase activity 20000IU/mL, galactosidase activity 200IU/mL, acid protease activity 400IU/mL, cellulase activity 100IU/mL and manna oligosacchride 0.07% (w/w).Get above-mentioned various enzyme preparation, make complex enzyme formulation by aforementioned technology after, get the manna oligosacchride liquid preparation again, carry out compositely according to the final ratio of 0.07% (w/w) and above-mentioned complex enzyme formulation, obtain the composite feed additive of composition proportion as mentioned above.
The application of embodiment 4 composite feed additives in fleshy duck fodder
The commodity of getting nascent 2 ages in days are divided into 6 groups at random for 1800 of ducklings, and 300 every group, wherein 4 groups is test group, and 4 groups is control group.Two groups are all adopted China Agricultural Cultivation Import ﹠ Export Co. that fleshy duck fodder is provided, carry out isolated rearing.Test group is added the product of the present invention of 0.05% (w/w), and control group does not add.Carry out feeding and management routinely, per two weeks weigh once, relatively weightening finish and the feed consumption situation of two groups of meat ducks.Result of the test shows (table 1, table 2), and test group increases weight obviously more than control group, and average growth rate surpasses 8%; During 6 ages in week, the feedstuff-meat ratio of test group reduces to 2.59 by 2.78 of control group, and the price of deed obviously is dominant.And, reached listing criteria in advance in 2～3 days than control group because the test group weightening finish is very fast.
Table 1 test group and control group meat duck body weight (unit: g)
| Group | Initially | 2 ages in week | 6 ages in week | |||
| Test group | Control group | Test group | Control group | Test group | Control group | |
| 1 | 78 | 77 | 481 | 458 | 2687 | 2379 |
| 2 | 76 | 78 | 492 | 425 | 2604 | 2451 |
| 3 | 81 | 80 | 472 | 451 | 2572 | 2422 |
| On average | 78 | 78 | 482 | 445 | 2621 | 2417 |
| Rate of body weight gain | 8.3％ | 8.4％ | ||||
Table 2 test group and control group meat duck feed consumption and feedstuff-meat ratio (unit: g)
| 2 ages in week | 6 ages in week | |||||||
| Group | Test group | Control group | Test group | Control group | ||||
| Feed consumption | Feedstuff-meat ratio | Feed consumption | Feedstuff-meat ratio | Feed consumption | Feedstuff-meat ratio | Feed consumption | Feedstuff-meat ratio | |
| 1 | 671 | 1.40 | 688 | 1.50 | 6715 | 2.50 | 6721 | 2.83 |
| 2 | 668 | 1.36 | 679 | 1.60 | 6877 | 2.64 | 6682 | 2.73 |
| 3 | 655 | 1.39 | 663 | 1.47 | 6755 | 2.63 | 6723 | 2.78 |
| On average | 665 | 1.38 | 677 | 1.52 | 6782 | 2.59 | 6709 | 2.78 |
Embodiment 5 composite feed additives are to the influence test of fryer enteric microorganism
Choose 1000 of the healthy fryer of body weight basically identical, be divided into 6 groups at random by 200 every group, the 1st group is contrast (basal diet of only feeding), and the 2nd group is added 4mg/kg flavomycoin, the 3rd, 4,5 group of product of the present invention (0.5g/kg, 1.0g/kg, 1.5g/kg) that adds various dose respectively in basal diet.Since 11 ages in days, feed to listing.
Last 1 day of test is got 5 moderate healthy fryer of body weight at random for every group and is butchered, and cuts open the belly immediately, gets each one section in blind, ileum, detects the content of Escherichia coli, Bacillus acidi lactici, Bifidobacterium and salmonella in each intestinal segment content.The result shows (table 3), and the Bifidobacterium quantity in caecum and the ileum is significantly improved (p＜0.05), and the Bacillus acidi lactici cultivation effect also has raising in various degree, and Escherichia coli quantity does not have notable difference; Each group does not all detect salmonella.Presentation of results, product of the present invention have effectively improved the microecosystem of meat duck enteron aisle.
Table 3 manna oligosacchride is to the influence (1g-CFU/g of unit) of fryer enteric microorganism
| Group | Bifidobacterium | Bacillus acidi lactici | Escherichia coli | |||
| Caecum | Ileum | Caecum | Ileum | Caecum | Ileum | |
| 1 | 9.14 | 8.53 | 9.05 | 6.35 | 8.59 | 7.95 |
| 2 | 9.34 | 9.09 | 9.17 | 6.23 | 8.88 | 8.66 |
| 3 | 9.95 | 8.94 | 9.69 | 4.26 | 9.24 | / |
| 4 | 10.21 | 9.46 | 9.03 | 6.29 | 8.73 | 8.21 |
| 5 | 9.64 | 9.40 | 9.12 | 6.82 | 8.65 | 6.22 |
Embodiment 6 composite feed additives are to the influence test of immune functions in broiler chickens
The grouping of test and the management and preceding identical of feeding.11 age in days collunarium eye droppings inoculation newcastle disease vaccine, 22 ages in days are randomly drawed 8 for every group, wing vein sterile blood sampling 2mL, separation of serum.Adopt hemagglutination test (HA test) and hemagglutination inhibition test (HI test) 96U shape plate reaction method to measure antibody titer.Adopt A-acetic acid naphthalene ester (ANAE) decoration method to measure the T lymphocyte.During off-test, randomly draw 3 fryer for every group, wing vein sterile blood sampling adopts full-automatic specific protein of Backman Array-360 and Pharmaceutical Analysis system, measures the content of IgA, IgG, IgM in the serum with the rate scattering immunoturbidimetry.Result's (table 4) shows, adds product of the present invention after, newcastle epidemic disease antibody is tired, T lymphocyte content, IgA, IgG and all obviously raisings of IgM content, shows that product of the present invention can effectively improve the cellular immunity and the humoral immunity ability of fryer.
Table 4 manna oligosacchride is to the immune influence of fryer
| Handle | Newcastle epidemic disease antibody is tired | The T lymphocyte, % | IgA，mg/L | IgG，mg/L | IgM，mg/L |
| 1 | 4.56 | 32.80 | 50.90 | 47.00 | 80.90 |
| 2 | 6.50 | 43.00 | 44.80 | 39.00 | 52.00 |
| 3 | 4.88 | 37.70 | 53.00 | 46.00 | 91.60 |
| 4 | 5.62 | 50.00 | 61.70 | 56.00 | 101.80 |
| 5 | 5.50 | 42.25 | 48.40 | 41.00 | 71.64 |
Claims (7)
1. highly effective biological zyme micro-zoology composite feed additive, the composition that it is characterized in that this composite feed additive is: with the NSP enzyme is that main enzyme is, acid protease, cellulase are auxiliary enzymes system, are equipped with the function oligosaccharides probiotics with antibiotic effect.
2. according to claim 1, it is characterized in that described NSP enzyme is mannase, zytase, galactosidase.
3. according to claim 1, it is characterized in that described function oligosaccharides probiotics is manna oligosacchride or wood oligose.
4. as described in the claim 1,2,3, it is characterized in that described composite feed additive includes following composition: beta-mannase enzymatic activity 3000～60000IU/mL, xylanase activity 2000～100000IU/mL, galactosidase activity 100～10000IU/mL, acid protease activity 100～1000IU/mL, cellulase activity 50～300IU/mL and manna oligosacchride 0.01～0.8% (w/w).
5. as described in the claim 1,2,3, it is characterized in that described composite feed additive includes following composition: beta-mannase enzymatic activity 3000～60000IU/mL, xylanase activity 2000～100000IU/mL, galactosidase activity 100～10000IU/mL, acid protease activity 100～1000IU/mL, cellulase activity 50～300IU/mL and wood oligose 0.01～0.8% (w/w).
6. as described in the claim 4, it is characterized in that described composite feed additive includes following optimization composition: beta-mannase enzymatic activity 10000IU/mL, xylanase activity 60000IU/mL, galactosidase activity 300IU/mL, acid protease activity 400IU/mL, cellulase activity 100IU/mL and manna oligosacchride 0.03% (w/w).
7. as described in the claim 5, it is characterized in that described composite feed additive includes following optimization composition: beta-mannase enzymatic activity 10000IU/mL, xylanase activity 60000IU/mL, galactosidase activity 300IU/mL, acid protease activity 400IU/mL, cellulase activity 100IU/mL and wood oligose 0.07% (w/w).
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102132770A (en) * | 2011-02-18 | 2011-07-27 | 青岛根源生物技术集团有限公司 | Corn bean pulp type feed complex enzyme additive and application thereof |
| CN101690545B (en) * | 2009-09-07 | 2012-10-17 | 肇东市日成酶制剂有限公司 | Method for producing complex micro-ecological preparation with microbial agents and enzyme |
| CN103493987A (en) * | 2013-09-29 | 2014-01-08 | 广东大华农动物保健品股份有限公司 | Water-soluble complex enzyme preparation for preventing and treating diarrhea and promoting growth of livestock and poultry and preparation method thereof |
| CN103564198A (en) * | 2013-10-14 | 2014-02-12 | 济南天天香有限公司 | Feeding solid mannase and application of feeding solid mannase to feed containing palm dregs |
| CN106173207A (en) * | 2016-07-15 | 2016-12-07 | 广州市博仕奥生物科技有限公司 | A kind of complex enzyme formula based on feeding guar meal and application thereof |
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2006
- 2006-09-01 CN CNA2006100374151A patent/CN101133779A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690545B (en) * | 2009-09-07 | 2012-10-17 | 肇东市日成酶制剂有限公司 | Method for producing complex micro-ecological preparation with microbial agents and enzyme |
| CN102132770A (en) * | 2011-02-18 | 2011-07-27 | 青岛根源生物技术集团有限公司 | Corn bean pulp type feed complex enzyme additive and application thereof |
| CN102132770B (en) * | 2011-02-18 | 2013-04-24 | 青岛根源生物技术集团有限公司 | Corn bean pulp type feed complex enzyme additive and application thereof |
| CN103493987A (en) * | 2013-09-29 | 2014-01-08 | 广东大华农动物保健品股份有限公司 | Water-soluble complex enzyme preparation for preventing and treating diarrhea and promoting growth of livestock and poultry and preparation method thereof |
| CN103493987B (en) * | 2013-09-29 | 2015-08-26 | 广东大华农动物保健品股份有限公司 | A kind ofly prevent and treat water-soluble complex enzyme preparation of livestock and poultry diarrhea and growth promoting effects and preparation method thereof |
| CN103564198A (en) * | 2013-10-14 | 2014-02-12 | 济南天天香有限公司 | Feeding solid mannase and application of feeding solid mannase to feed containing palm dregs |
| CN103564198B (en) * | 2013-10-14 | 2015-06-03 | 济南天天香有限公司 | Feeding solid mannase and application of feeding solid mannase in feed containing palm dregs |
| CN106173207A (en) * | 2016-07-15 | 2016-12-07 | 广州市博仕奥生物科技有限公司 | A kind of complex enzyme formula based on feeding guar meal and application thereof |
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Application publication date: 20080305 |