CN106171988B - Improve quick reproductive survival rate culture medium of blueberry detoxic seedling and preparation method thereof - Google Patents
Improve quick reproductive survival rate culture medium of blueberry detoxic seedling and preparation method thereof Download PDFInfo
- Publication number
- CN106171988B CN106171988B CN201610551412.3A CN201610551412A CN106171988B CN 106171988 B CN106171988 B CN 106171988B CN 201610551412 A CN201610551412 A CN 201610551412A CN 106171988 B CN106171988 B CN 106171988B
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- Prior art keywords
- blueberry
- culture medium
- survival rate
- seedling
- detoxic seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The present invention relates to a kind of culture mediums, and in particular to a kind of raising quick reproductive survival rate culture medium of blueberry detoxic seedling and preparation method thereof.The culture medium, minimal medium is WPM, and added with 0.2 1.0g/L lactoalbumin hydrolysates, 0.4 1.2g/L vitamin Es, 0.002 0.006g/L zeatin, 0.002 0.008g/L, 6 benzyladenines, 0.003 0.01g/L methyl α-naphthyl acetates, 0.004 0.02g/L, 2,4 dichlorphenoxyacetic acids and 10 40g/L sucrose.The culture medium can ensure good growth conditions and high survival rate of the blueberry detoxic seedling in tissue-culturing rapid propagation, and making detoxic seedling for blueberry tissue culture lays a good foundation；The preparation method, it is simple and practicable, easy to operation.
Description
Technical field
The present invention relates to a kind of culture mediums, and in particular to a kind of raising quick reproductive survival rate culture medium of blueberry detoxic seedling
And preparation method thereof.
Background technology
Blueberry is a kind of small berries, and fruit is in blue, pulp is fine and smooth, seed is minimum, has " king of berry " and " fruit emperor
Good reputation afterwards ".Blueberry averagely weighs 0.5~2.5g, maximum weight 5g, edible rate 100%, sweet and sour palatability, and feels well with fragrant
Pleasant fragrance, to eat good merchantable brand raw.Blueberry is not only rich in conventional nutrients, such as proteins,vitamins,minerals and micro member
Element etc., but also containing extremely abundant anthocyanidin, flavonoids and polysaccharide compound, have and alleviate asthenopia, promote to regard
Visual purple regeneration in retinulae, pre- myopia prevention improve and promote eyesight and other effects.
In addition to asthenopia is alleviated, blueberry also has many healthcare functions, such as prevents cardiovascular and cerebrovascular disease, anti-cancer, raising
Immunity, alleviation is had sore throat and diarrhea etc., so, utilization and spin-off exploitation to blueberry are increasingly taken seriously.
In view of the broad mass market prospect of blueberry, is also increasingly taken seriously to the research and development of blueberry nursery stock.It is trained in blueberry nursery stock
In educating, cuttage and tissue culture detoxicating mode can be passed through.Cuttage seeding causes a timing due to that may contain viral germ etc.
Between after blueberry quality deterioration, so as to influence the yield and quality of blueberry and economic benefit.Therefore, to prevent blueberry quality deterioration from showing
As occurring, ensure the quality of blueberry and yield and the economic benefit of blueberry manufacturing enterprise, by make blueberry detoxic seedling carry out it is numerous
Educating just becomes outstanding science-and-technology enterprise or the preferred varieties breeding mode of scientific and technical research personnel.
Detoxic seedling makes, and the general blueberry stem apex or leaf bud etc. chosen has the separate living tissue of stronger meristematic capacity, in reality
It tests in room by sterilizing except virus treated, is then inoculated in sterile virus-free culture medium, by gradually cultivating, then observes
The contamination microbiological contamination situation of blueberry seedling filters out the blueberry seedling without virus and bacterial invasion, passes through modern biotechnology means
Further segment is numerous soon, so as to fulfill the purpose of a large amount of blueberry detoxic seedlings is obtained.
In blueberry detoxic seedling manufacturing process, how to ensure the detoxic seedling obtained can fast-growth breeding be that blueberry takes off
Key factor whether malicious seedling is successful.
Invention content
In view of the deficiencies of the prior art, the object of the present invention is to provide a kind of raising quick reproductive survival rates of blueberry detoxic seedling
With culture medium, blueberry detoxic seedling can be made to keep good growth conditions and high survival rate during tissue-culturing rapid propagation, be
Blueberry tissue culture makes detoxic seedling and lays the foundation；Present invention simultaneously provides preparation methods.
The raising quick reproductive survival rate culture medium of blueberry detoxic seedling of the present invention, minimal medium WPM, and add
Added with 0.2-1.0g/L lactoalbumin hydrolysates, 0.4-1.2g/L vitamin Es, 0.002-0.006g/L zeatin, 0.002-0.008g/
L 6-benzyladenines, 0.003-0.01g/L methyl α-naphthyl acetates, 0.004-0.02g/L 2,4 dichlorophenoxyacetic acids and 10-40g/L sugarcanes
Sugar.
The culture medium is preferably：Minimal medium is WPM, and is tieed up added with 0.5g/L lactoalbumin hydrolysates, 0.6g/L
Raw element E, 0.003g/L zeatin, 0.004g/L 6-benzyladenines, 0.004g/L methyl α-naphthyl acetates, 0.008g/L 2,4 dichloro benzenes
Fluoroacetic acid and 20g/L sucrose.
The preparation method of the raising quick reproductive survival rate culture medium of blueberry detoxic seedling, by lactoalbumin hydrolysate, dimension
Raw element E, zeatin, 6-benzyladenine, methyl α-naphthyl acetate, 2,4- dichlorphenoxyacetic acids and sucrose are added in minimal medium, are mixed
It is dispensed after closing uniformly, it is spare after sterilizing.
The packing specification is 20-30mL/ bottles.
Compared with prior art, beneficial effects of the present invention are as follows：
(1) in the present invention, WPM minimal mediums provide nutritional ingredient needed for the growth of blueberry tissue culture seedling, and vitamin
E, lactoalbumin hydrolysate have the function of inhibition phenoloxidase activity, and a combination thereof can mitigate tissue-cultured seedling metabolite to blueberry seedling
Toxic action, lactoalbumin hydrolysate can also supply nutrient needed for seedling as nutrient；Zeatin, 6-benzyladenine, naphthalene
Acetic acid and 2,4- dichlorphenoxyacetic acid etc. are used as plant hormone ingredient, have and promote cell division, and root system is promoted to be formed, and promote leaf
Functions, four synergy such as bud differentiation ensure that the quick division growth of blueberry tissue culture seedling；Sucrose provides not only blueberry group
The carbon supply needed for growth of seedling is trained, while Premeabilisation of cells pressure can be adjusted, ensure that the normal growth of blueberry bottle seedling.
(2) the raising quick reproductive survival rate culture medium of blueberry detoxic seedling of the present invention, can ensure blueberry detoxification
Good growth conditions and high survival rate of the seedling in tissue-culturing rapid propagation make detoxic seedling for blueberry tissue culture and have established base
Plinth.
(3) preparation method of the present invention, it is simple and practicable, easy to operation.
Specific embodiment
With reference to embodiment, the present invention will be further described.
The minimal medium WPM used in embodiment is prepared using those skilled in the art's well-known formulations.
Embodiment 1
The quick reproductive survival rate culture medium of raising blueberry detoxic seedling, minimal medium WPM, and be added with
0.5g/L lactoalbumin hydrolysates, 0.6g/L vitamin Es, 0.003g/L zeatin, 0.004g/L 6-benzyladenines, 0.004g/L
Methyl α-naphthyl acetate, 0.008g/L 2,4 dichlorophenoxyacetic acids and 20g/L sucrose.
Preparation method is：
By lactoalbumin hydrolysate, vitamin E, zeatin, 6-benzyladenine, methyl α-naphthyl acetate, 2,4 dichlorophenoxyacetic acid and sugarcane
Sugar is added in minimal medium, is dispensed after mixing, spare after sterilizing.
Packing specification is 25mL/ bottles.
Embodiment 2
The quick reproductive survival rate culture medium of raising blueberry detoxic seedling, minimal medium WPM, and be added with
0.2g/L lactoalbumin hydrolysates, 0.4g/L vitamin Es, 0.002g/L zeatin, 0.002g/L 6-benzyladenines, 0.003g/L
Methyl α-naphthyl acetate, 0.004g/L 2,4 dichlorophenoxyacetic acids and 10g/L sucrose.
Preparation method is：
By lactoalbumin hydrolysate, vitamin E, zeatin, 6-benzyladenine, methyl α-naphthyl acetate, 2,4 dichlorophenoxyacetic acid and sugarcane
Sugar is added in minimal medium, is dispensed after mixing, spare after sterilizing.
Packing specification is 20mL/ bottles.
Embodiment 3
Minimal medium is WPM, and added with 1.0g/L lactoalbumin hydrolysates, 1.2g/L vitamin Es, 0.006g/L corns
Element, 0.008g/L 6-benzyladenines, 0.01g/L methyl α-naphthyl acetates, 0.02g/L 2,4 dichlorophenoxyacetic acids and 40g/L sucrose.
Preparation method is：
By lactoalbumin hydrolysate, vitamin E, zeatin, 6-benzyladenine, methyl α-naphthyl acetate, 2,4 dichlorophenoxyacetic acid and sugarcane
Sugar is added in minimal medium, is dispensed after mixing, spare after sterilizing.
Packing specification is 30mL/ bottles.
Blueberry seedling is inoculated into respectively on culture medium prepared by WPM minimal mediums and embodiment 1-3, is observed after 5 weeks
Growing state of the blueberry seedling on different culture media, is specifically shown in Table 1.Situation is survived to be represented with survival rate.Survival rate calculation formula
For：The total blueberry seedling number being inoculated in the blueberry seedling number survived in survival rate=each culture bottle/each culture bottle.
The growing state of blueberry seedling on 1 different culture media of table
| Every bottle of inoculation sum | Survive a number | Survival rate (%) | |
| WPM minimal mediums | 30 | 8 | 26.7 |
| 1 culture medium of embodiment | 30 | 30 | 100 |
| 2 culture medium of embodiment | 30 | 26 | 86.7 |
| 3 culture medium of embodiment | 30 | 24 | 80 |
Observation is found：Embodiment 1-3 prepare culture medium can significantly improve blueberry detoxication and fast reproduction tissue-cultured seedling into
Motility rate improves the growth conditions of bottle seedling.The growing state of blueberry seedling on embodiment 1-3 culture mediums is far superior to minimal medium
On, the blueberry seedling growing state wherein on 1 culture medium of embodiment is optimal.
Claims (4)
1. a kind of raising quick reproductive survival rate culture medium of blueberry detoxic seedling, it is characterised in that：Minimal medium is WPM, and
Added with 0.2-1.0g/L lactoalbumin hydrolysates, 0.4-1.2g/L vitamin Es, 0.002-0.006g/L zeatin, 0.002-
0.008g/L6- benzyladenines, 0.003-0.01g/L methyl α-naphthyl acetates, 0.004-0.02g/L 2,4 dichlorophenoxyacetic acids and 10-
40g/L sucrose.
2. the raising quick reproductive survival rate culture medium of blueberry detoxic seedling according to claim 1, it is characterised in that：Substantially
Culture medium is WPM, and added with 0.5g/L lactoalbumin hydrolysates, 0.6g/L vitamin Es, 0.003g/L zeatin, 0.004g/L 6-
Benzyladenine, 0.004g/L methyl α-naphthyl acetates, 0.008g/L 2,4 dichlorophenoxyacetic acids and 20g/L sucrose.
3. a kind of preparation method of the raising quick reproductive survival rate culture medium of blueberry detoxic seedling described in claims 1 or 2,
It is characterized in that：By lactoalbumin hydrolysate, vitamin E, zeatin, 6-benzyladenine, methyl α-naphthyl acetate, 2,4 dichlorophenoxyacetic acid and sugarcane
Sugar is added in minimal medium, is dispensed after mixing, spare after sterilizing.
4. the preparation method according to claim 3 for improving the quick reproductive survival rate culture medium of blueberry detoxic seedling, special
Sign is：The packing specification is 20-30mL/ bottles.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610551412.3A CN106171988B (en) | 2016-07-13 | 2016-07-13 | Improve quick reproductive survival rate culture medium of blueberry detoxic seedling and preparation method thereof |
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| CN201610551412.3A CN106171988B (en) | 2016-07-13 | 2016-07-13 | Improve quick reproductive survival rate culture medium of blueberry detoxic seedling and preparation method thereof |
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| Publication Number | Publication Date |
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| CN106171988A CN106171988A (en) | 2016-12-07 |
| CN106171988B true CN106171988B (en) | 2018-06-22 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110432133A (en) * | 2019-08-27 | 2019-11-12 | 吉林农业大学 | A kind of blueberry rapid breeding method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004754A (en) * | 2012-12-14 | 2013-04-03 | 苏州和美生物科技有限公司 | Composition for preventing browning of plant tissue culture and using method of composition |
| CN103404434A (en) * | 2013-08-05 | 2013-11-27 | 浙江省农业科学院 | Prevention technology for multiple blueberry viruses at early stage |
| CN103583368A (en) * | 2013-11-22 | 2014-02-19 | 西南林业大学 | Construction method for vaccinium ashei tissue culture rapid propagation system |
| CN104823857A (en) * | 2015-05-18 | 2015-08-12 | 江苏农林职业技术学院 | Seedling raising method for promoting ex-vitro rooting of blueberry tissue culture seedlings |
-
2016
- 2016-07-13 CN CN201610551412.3A patent/CN106171988B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004754A (en) * | 2012-12-14 | 2013-04-03 | 苏州和美生物科技有限公司 | Composition for preventing browning of plant tissue culture and using method of composition |
| CN103404434A (en) * | 2013-08-05 | 2013-11-27 | 浙江省农业科学院 | Prevention technology for multiple blueberry viruses at early stage |
| CN103583368A (en) * | 2013-11-22 | 2014-02-19 | 西南林业大学 | Construction method for vaccinium ashei tissue culture rapid propagation system |
| CN104823857A (en) * | 2015-05-18 | 2015-08-12 | 江苏农林职业技术学院 | Seedling raising method for promoting ex-vitro rooting of blueberry tissue culture seedlings |
Non-Patent Citations (2)
| Title |
|---|
| 兔眼蓝莓快繁技术研究;朱芳明等;《西南林业大学学报》;20160630;第36卷(第3期);第73-79页 * |
| 越橘组织培养研究进展;梁晓晶等;《黑龙江农业科学》;20111231(第6期);第138-142页，尤其是第139页第4段1.1.3节 * |
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| CN106171988A (en) | 2016-12-07 |
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