CN106212286B - A kind of laurustinus special culture media - Google Patents
A kind of laurustinus special culture media Download PDFInfo
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- CN106212286B CN106212286B CN201610653568.2A CN201610653568A CN106212286B CN 106212286 B CN106212286 B CN 106212286B CN 201610653568 A CN201610653568 A CN 201610653568A CN 106212286 B CN106212286 B CN 106212286B
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- nitrate
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- culture medium
- earth compound
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The present invention relates to a kind of laurustinus special culture medias, including mother liquor 60-65 parts of the basis MS, 4-6 parts of glucose, 6-8 parts of lactoalbumin hydrolysate, 4-8 parts of corn embryosperm, Tomato juice 4-8 parts, 2-14 parts of coconut milk, 2-5 parts of indolebutyric acid, 1-4 parts of methyl α-naphthyl acetate, 2-6 parts of ABT root-inducing powder, 5-15 parts of rare-earth compound, 5-10 parts of active carbon, 30-35 parts of water.The present invention has simple and easy feature more than quantity of emerging, and provides technical support for industrial seedling rearing.
Description
Technical field
The present invention relates to plant culture field, especially a kind of laurustinus special culture media.
Background technique
Silk ball is good also known as hydrangea, hydrangea, is Saxifragaceae Hydrangea plant.Laurustinus flower buy it is white plentiful, it is big and
Beauty, pattern energy is red can be blue, makes us pleasing and soothes the spirit, is common potting ornamencal flower and tree.
Silk ball spent culture medium is less at present, and it is not easy to operate, seedling effect is bad, it is difficult to meet the market requirement.
Summary of the invention
Present invention aims to a kind of for laurustinus special culture media in providing.For in the prior art it is not easy to operate, go out
The problems such as seedling is ineffective, the present invention provides a kind of practicable solutions.The present invention has simple and easy, number of emerging
The more feature of amount, provides technical support for industrial seedling rearing.
The present invention be reach its purpose, the technical solution adopted is as follows:
A kind of laurustinus special culture media, the laurustinus special culture media include mother liquor 60-65 parts of the basis MS, glucose
4-6 parts, 6-8 parts of lactoalbumin hydrolysate, 4-8 parts of corn embryosperm, Tomato juice 4-8 parts, 2-14 parts of coconut milk, 2-5 parts of indolebutyric acid,
1-4 parts of methyl α-naphthyl acetate, 2-6 parts of ABT root-inducing powder, 5-15 parts of rare-earth compound, 5-10 parts of active carbon, 30-35 parts of water.
Further, the rare-earth compound for including in culture medium is lanthanum nitrate, neodymium nitrate, at least one in samaric nitrate
Kind.
Further, rare-earth compound is added to culture medium as follows: in superclean bench environment, by nitre
One of sour lanthanum, neodymium nitrate, samaric nitrate are dissolved in water and form solution, through filter-sterilizer filtration sterilization, while will be not added with dilute
The culture medium of earth elements compound carries out high-temperature sterilization, before culture medium not yet restores room temperature, with liquid-transfering gun by lanthanum nitrate or nitre
The solution of sour neodymium or samaric nitrate is dispensed into each culture medium, and is rocked uniformly.
Further, laurustinus special culture media include 60 parts of the basis MS mother liquor, 4 parts of glucose, 6 parts of lactoalbumin hydrolysate,
4 parts of corn embryosperm, 4 parts of Tomato juice, 4 parts of coconut milk, 2 parts of indolebutyric acid, 2 parts of methyl α-naphthyl acetate, 3 parts of ABT root-inducing powder, rare earth element
7 parts of compound, 6 parts of active carbon, 32 parts of water.Wherein, rare-earth compound is the mixture of lanthanum nitrate and neodymium nitrate, wherein nitre
The mass ratio of sour lanthanum and neodymium nitrate is 3:4.Wherein, rare-earth compound may be lanthanum nitrate, neodymium nitrate and samaric nitrate
Mixture, wherein the mass ratio 2:2:3 of lanthanum nitrate, neodymium nitrate and samaric nitrate.
Laurustinus special culture media in the present invention is primarily as adventitious bud culture medium；In the present invention it is further proposed that
Laurustinus plantlet culture medium, the laurustinus plantlet with culture medium be mother liquor 45-50 parts of the basis MS, 2-5 parts of glucose,
4-6 parts of lactoalbumin hydrolysate, 2-4 parts of corn embryosperm, 2-4 parts of peach juice, 2-10 parts of coconut milk, 2-5 parts of heteroauxin, naphthyloxyacetic acid 1-
4 parts, 2-6 parts of ABT root-inducing powder, 5-15 parts of rare-earth compound, 5-10 parts of active carbon, 20-30 parts of water.
Further, the laurustinus plantlet is lanthanum nitrate, neodymium nitrate, nitre with the rare-earth compound in culture medium
At least one of sour samarium.
Further, laurustinus plantlet culture medium includes 45 parts of the basis MS mother liquor, 2 parts of glucose, lactoalbumin hydrolysate 4
Part, 2 parts of corn embryosperm, 3 parts of peach juice, 2 parts of coconut milk, 2 parts of heteroauxin, 2 parts of naphthyloxyacetic acid, 3 parts of ABT root-inducing powder, rare earth member
6 parts of plain compound, 5 parts of active carbon, 30 parts of water.
The beneficial effects of the present invention are:
Rare-earth compound lanthanum nitrate, neodymium nitrate, samaric nitrate are added in the present invention into conventional medium, low concentration
Lanthanum nitrate, neodymium nitrate, samaric nitrate can stimulate the meristematic zone of root, promote tissue culture seedling rooting, obtain sturdy root system, improve seedling
Transplanting survival rate；It can also promote the growth of Multiple Buds simultaneously, increase laurustinus tissue-cultured seedling emergence quantity, improve emergence rate, from
And tissue-cultured seedling cost is reduced, be conducive to a large amount of outputs of laurustinus in this way.
Specific embodiment
The present invention will be further described combined with specific embodiments below.
Embodiment 1
A kind of silk ball spent culture medium, including 65 parts of the basis MS mother liquor, 5 parts of glucose, 5.5 parts of lactoalbumin hydrolysate, maize
Newborn 4.5 parts, 4 parts of Tomato juice, 4 parts of coconut milk, 2.75 parts of indolebutyric acid, 2.5 parts of methyl α-naphthyl acetate, 3.75 parts of ABT root-inducing powder, nitric acid
8 parts of lanthanum, 6.5 parts of active carbon, 35 parts of water.
Embodiment 2
A kind of silk ball spent culture medium, including 60 parts of the basis MS mother liquor, 4 parts of glucose, 6 parts of lactoalbumin hydrolysate, corn embryosperm 4
Part, 4 parts of Tomato juice, 4 parts of coconut milk, 2 parts of indolebutyric acid, 2 parts of methyl α-naphthyl acetate, 3 parts of ABT root-inducing powder, 3 parts of lanthanum nitrate, neodymium nitrate 4
Part, 6 parts of active carbon, 32 parts of water.
When preparing culture medium, it then follows following steps:
1. preparing the basis MS mother liquor.
2. weighing appropriate ABT root-inducing powder, lactoalbumin hydrolysate, corn embryosperm, glucose and active carbon, ABT root-inducing powder is fallen
Enter to boil in tap water and stir evenly, after being cooled to room temperature, adds lactoalbumin hydrolysate, corn embryosperm, glucose and active carbon
Until spare after dissolution.
3. the tomato of clean peeling is cut into small pieces, it is then placed in juice extractor and crushes, obtained homogenate is western red
Persimmon juice and peach juice.
4. by mother liquor, ABT root-inducing powder, lactoalbumin hydrolysate, corn embryosperm, glucose, active carbon, coconut milk, Tomato juice,
Indolebutyric acid, methyl α-naphthyl acetate after constant volume, are dispensed into culture bottle, high-temperature sterilization by a certain percentage.
5. rare-earth compound in superclean bench, is dissolved in sterile water and filtration sterilization, then dispensed with liquid-transfering gun
Into the sterilized culture medium of each bottle described in 4, shake up.
When preparing culture medium, in addition to various nutrient mother liquors are prepared using distilled water, other components be can be used certainly
Water is prepared, and can be simplified program indirectly and be reduced cost.
The technology being not directed in the specific embodiment of the invention belongs to techniques known, with reference to well-known technique plus
To implement.
Although above-described embodiment is described in detail technical solution of the present invention, technical side of the invention
Case is not limited to above embodiments, in the case where not departing from thought and objective of the invention, to technical solution of the present invention institute
Any change done falls within claims of the present invention limited range.
Claims (3)
1. a kind of laurustinus special culture media, it is characterised in that: the laurustinus special culture media is by the basis MS mother liquor 60-65
Part, 4-6 parts of glucose, 6-8 parts of lactoalbumin hydrolysate, 4-8 parts of corn embryosperm, Tomato juice 4-8 parts, 2-14 parts of coconut milk, indoles
2-5 parts of butyric acid, 1-4 parts of methyl α-naphthyl acetate, 2-6 parts of ABT root-inducing powder, 5-15 parts of rare-earth compound, 5-10 parts of active carbon, water 30-
35 parts of compositions；The rare-earth compound is the mixture of lanthanum nitrate and neodymium nitrate, wherein the quality of lanthanum nitrate and neodymium nitrate
Than for 3:4；Rare-earth compound is added to culture medium as follows: in superclean bench environment, by lanthanum nitrate, nitric acid
Neodymium is dissolved in water and forms solution, carries out through filter-sterilizer filtration sterilization, while by the culture medium for being not added with rare-earth compound
The solution is added in culture medium with liquid-transfering gun, and rocks uniformly before culture medium not yet restores room temperature for high-temperature sterilization.
2. a kind of laurustinus special culture media, it is characterised in that: the laurustinus special culture media is by the basis MS mother liquor 60-65
Part, 4-6 parts of glucose, 6-8 parts of lactoalbumin hydrolysate, 4-8 parts of corn embryosperm, Tomato juice 4-8 parts, 2-14 parts of coconut milk, indoles
2-5 parts of butyric acid, 1-4 parts of methyl α-naphthyl acetate, 2-6 parts of ABT root-inducing powder, 5-15 parts of rare-earth compound, 5-10 parts of active carbon, water 30-
35 parts of compositions；The rare-earth compound is the mixture of lanthanum nitrate, neodymium nitrate and samaric nitrate, wherein lanthanum nitrate, neodymium nitrate
With the mass ratio 2:2:3 of samaric nitrate；Rare-earth compound is added to culture medium as follows: in superclean bench environment
In, lanthanum nitrate, neodymium nitrate, samaric nitrate are dissolved in water and form solution, through filter-sterilizer filtration sterilization, while rare earth will be not added with
The culture medium of element compound carries out high-temperature sterilization and the solution is added with liquid-transfering gun before culture medium not yet restores room temperature
In culture medium, and rock uniformly.
3. silk ball spent culture medium according to claim 1 or 2, it is characterised in that: the laurustinus special culture media is by MS
60 parts of basic mother liquor, 4 parts of glucose, 6 parts of lactoalbumin hydrolysate, 4 parts of corn embryosperm, 4 parts of Tomato juice, 4 parts of coconut milk, indoles fourth
2 parts of acid, 2 parts of methyl α-naphthyl acetate, 3 parts of ABT root-inducing powder, 7 parts of rare-earth compound, 6 parts of active carbon, 32 parts of water compositions.
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| CN201610653568.2A CN106212286B (en) | 2016-08-10 | 2016-08-10 | A kind of laurustinus special culture media |
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| CN201610653568.2A CN106212286B (en) | 2016-08-10 | 2016-08-10 | A kind of laurustinus special culture media |
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| CN106212286B true CN106212286B (en) | 2019-06-25 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108040881A (en) * | 2017-12-28 | 2018-05-18 | 广西南宁桂尔创环保科技有限公司 | A kind of dragon fruit synchronization tissue culture culture medium and the cultural method of plant |
| CN107980466A (en) * | 2017-12-29 | 2018-05-04 | 福建省林业科学研究院 | A kind of fruit of a cubeb litsea tree tissue-cultured seedling minitype cuttage method |
| CN109287483B (en) * | 2018-11-02 | 2022-03-25 | 杭州市园林绿化股份有限公司 | Method for quickly cultivating hydrangea macrophylla container seedlings |
| CN110839529A (en) * | 2019-11-22 | 2020-02-28 | 江苏东郁植物科技有限公司 | Hydrangea liquid culture medium tissue culture rapid propagation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0325816A1 (en) * | 1988-01-05 | 1989-08-02 | Leo Anne Eveleens | Method for forcing hydrangea plants into bloom all year round |
| CN101250497A (en) * | 2008-04-14 | 2008-08-27 | 潍坊学院 | Culture media for plant tissue culture |
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2016
- 2016-08-10 CN CN201610653568.2A patent/CN106212286B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0325816A1 (en) * | 1988-01-05 | 1989-08-02 | Leo Anne Eveleens | Method for forcing hydrangea plants into bloom all year round |
| CN101250497A (en) * | 2008-04-14 | 2008-08-27 | 潍坊学院 | Culture media for plant tissue culture |
Non-Patent Citations (2)
| Title |
|---|
| 绣球花的植物组织培养和快速繁殖技术;吴华芬等;《浙江农业科学》;20091231(第1期);第90-91页 |
| 绣球花的组织培养（简报）;江奕凤;《亚热带植物通讯》;19981231;第27卷(第1期);第53页第3段 |
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