CN108040880A - A kind of synchronous cultural method of dragon fruit plant - Google Patents
A kind of synchronous cultural method of dragon fruit plant Download PDFInfo
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- CN108040880A CN108040880A CN201711461482.0A CN201711461482A CN108040880A CN 108040880 A CN108040880 A CN 108040880A CN 201711461482 A CN201711461482 A CN 201711461482A CN 108040880 A CN108040880 A CN 108040880A
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- dragon fruit
- plant
- seedling
- charcoal
- root
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The present invention provides a kind of synchronous cultural method of dragon fruit plant, tissue cultures are carried out using dragon fruit cotyledonary node, add the generation synchronous with adventitious root of ethylene evoking adventive bud, break plant tissue dormancy, promote the growth of bud and root, nitrate rare earth element and charcoal are utilized simultaneously, promote the synthesis of chloroplaset, improve the respiration of aseptic plant, charcoal accelerates root growth, improve the utilization rate of culture medium, the addition of humic acid promotes absorption of the root system to nitrate rare earth element, so as to shorten entire cultivation cycle, improve the adaptability of plant hardening, shorten entire cultivation cycle.The dragon fruit plant cultivation cycle of the present invention is short, adaptable, substantially reduces toxigenic capacity.
Description
Technical field
The invention belongs to technical field of plant propagation, and in particular to a kind of method of dragon fruit tissue cultures.
Background technology
Dragon fruit is with health role, has effects that purify the blood, fall fire norcholesterol, and often feeding can promote human body new old generation
It thanks, improves immune function, its cultivation in addition is relatively easy to, and pest and disease damage is few, and early fruiting and high yield adapts to arid climate, is that kind is great
The novel fruits of market development potential.In recent years, the kind of dragon fruit is being greatly developed in the provinces such as Yunnan, Guizhou, Guangxi, Guangdong
The industries such as plant, processing, tourist sight.In this context, dragon fruit seedling market is hot, and supply falls short of demand for seedling.
At present, the mode of dragon fruit seeling industry has seminal propagation, cutting propagation and tissue culture propagation, and wherein tissue cultures are
One effective way of quick breeding production dragon fruit seedling.Existing dragon fruit group culturation rapid propagating technology generally using dragon fruit stem section as
Explant through adventitious bud inducing, is proliferated and three steps of taking root complete plant regenerations.There is the cells during Multiplying culture
The mitogen concentration excessively high and entire production cycle is long（As conventional three-step approach regeneration plant is moved from explant bottle inlet to bottle seedling
Plant the needs time of at least 110 days）Two big shortcomings, the seedling aberration rate height of thus caused production, quality is unstable, is produced into
The problems such as this is high seriously reduces dragon fruit tissue culture seedling and efficiency is factory produced, greatly limit the scale of its production.
Having will be proliferated and takes root what is synchronously carried out, but the tissue-cultured seedling of these tissue culture methods production only has base root, without aerial root（Aerial root
Refer to the root grown on the newly-increased side shoot of tissue-cultured seedling）, i.e. one plant of tissue-cultured seedling only has base root, and tissue-cultured seedling is one with the finished product seedling formed
One plant of finished product seedling at most can be only formed to one, one plant of tissue-cultured seedling, therefore, planting percent is low, and production cost is higher.
Patent of invention CN200910311410.7, a kind of method for tissue culture of dragon fruit, the ripe stem section warp of dragon fruit
It disinfects, is seeded on MS+ paclobutrazol 0.1 ~ 1.5mg/L axillary bud deriving culture mediums, the axillary bud of acquisition is cut and is transferred to MS+6-
It being proliferated on BA1.0 ~ 6.0mg/L+ cycocel 0.1 ~ 1.5mg/L culture mediums, 42% test tube seedling is synchronously taken root, and is directly used in transplanting,
Transplanting survival rate reaches more than 95%；The inventive method is suitble to industrialized production, and formulation efficacy is good, and budding is fast, the fire produced
Imperial fruit seedling has the advantages of genetic stability is high, workable, application value is high.Dragon fruit is enable to realize that batch production is quick
Nursery can more effectively develop dragon fruit.
Patent of invention CN201410135613.6, a kind of method for improving dragon fruit tissue-cultured seedling planting percent, with dragon fruit band
Thorn seat and to retain the semi-lignified stem piece of spinule and villus on thorn seat be explant, through adventitious bud inducing, is synchronously proliferated and takes root
Seedling culture, adventitious bud can grow side shoot, when adventitious bud base portion grows Ji Gen and side shoot grows aerial root, hardening, after hardening
Seedling out of bottle is taken out, side shoot with aerial root is cut and the stem with base root is planted in matrix, wait to survive grow it is new
Root can go up basin.This method can not only make that the dragon fruit tissue culture seeling industry cycle is short, adventitious bud induction frequency is high, is proliferated and takes root same
Step, growth coefficient reach more than 5.45, rooting rate and reach 100%, and multiplication seedling growing way is vigorous, and transplanting survival rate reaches more than 95%,
And an adventitious bud of inoculation can be made to grow 3 ~ 4 side shoots with aerial root, that is, an adventitious bud being inoculated with can breed 4 ~ 5
Seedling of the strain with root, dragon fruit tissue-cultured seedling planting percent improve 3 ~ 4 times.
Patent of invention CN201710081396.0, a kind of culture medium of dragon fruit tissue cultures, the component of the culture medium and
The content of each component is as follows：(1) inorganic nutrients ingredient：1. a great number of elements is 2. micro-；(2) Organic nutrient；(3) plant
Growth regulatory substance：Cytokinin 6-benzyladenine 0.55mg/L, auxins methyl α-naphthyl acetate 0.15mg/L；(4) its
His additive：Activated carbon 100mg/L；(5) white sugar 35g/L；(6) agar 3.0g/L；Surplus is distilled water.The culture medium was both
Regenerated plant, and root induction seedling simultaneously, i.e. simplified culture step can be cultivated, shoot proliferation culture and rooting induction culture are one
Step is completed, and greatly shortens the production cycle, cost-effective, the present invention provides a kind of cultures of efficient dragon fruit tissue cultures
Base, to solve the problem of that the production cycle occurred in dragon fruit cultivating process long production cost is high.
In the prior art, the research of dragon fruit tissue cultures is fewer and fewer, and the tissue cultures cycle is long, dragon fruit take root it is few into
Motility rate is low.
The content of the invention
For the problem that dragon fruit plant cultivation cycle is long in the prior art, survival rate of plant is low, and the present invention provides a kind of
The synchronous cultural method of dragon fruit plant.
The present invention is achieved by the following technical solutions：
1. a kind of synchronous cultural method of dragon fruit plant, comprises the following steps：
A. by the dragon fruit cotyledonary node of no disease and pests harm（Cut off seedling and part hypocotyl）Adventitious bud inducing is synchronously carried out in bottle
With take root, obtain aseptic seedlings；
B. aseptic seedling bottle stopper is opened, by aseptic seedlings ventilation and is interrupted illumination cultivation, obtains nascent plant；
C. after nascent plant being washed away culture medium with clear water, transplant into matrix, sprinkle profoundly water and be managed culture, both obtain dragon fruit
Plant；
The step A is specific as follows：
（1）The processing of seedling tissue
Dragon fruit edible tender branch is taken to clean up, with 75% concentration 30 ~ 60s of ethanol postincubation, aseptic water washing 1 ~ 2 time cuts off seedling
3 ~ 4cm cotyledonary nodes are obtained with part hypocotyl, marrow is removed, then is gone out with 10% ~ 20% NaClO and 0.1% ~ 0.5% Tween-40
Cotyledonary node after sterilizing is cut into width as 2cm ~ 3cm, the small pieces of a length of 3cm ~ 4cm by 15 ~ 20min of bacterium, aseptic water washing 4 ~ 5 times,
Obtain cotyledon nodal plate；
（2）The induction of adventitious bud, root
The cotyledon nodal plate handled well is inoculated into inducing culture, is 25 ~ 30 DEG C in temperature, intensity of illumination for 1500 ~
It is cultivated under 3000lux, 14 ~ 16h/d of light application time, obtains aseptic seedlings；
The inducing culture is 0.4 ~ 1.0mg/L of WPM+KT 1.0 ~ 3.0mg/L+NAA+2.0 ~ 4.0mg/ of ethylene
L+35 ~ 45g/L of tomato puree+6 ~ 8g/L of agar+5 ~ 15ml/L of earth solution+100 ~ 120g/L of charcoal+humic
Acid 10 ~ 20mg/L, pH are 6.0 ~ 6.5；
The step B is specific as follows：
When adventitious bud and adventitious root grow to 3 ~ 5cm, bottle seedling is moved in the greenhouse of outdoor ventilation, opens bottle stopper, hardening 4 ~ 6
My god, when bottle seedling grows 2 ~ 3 thorn seats, stop hardening, obtain nascent plant；
The step C is specific as follows：
After nascent plant is washed with water removal culture medium, plant in charcoal：Perlite=2:It is right before planting in 1 matrix
Matrix sprinkles profoundly water in advance, pours within 3 ~ 5 days a water, keeps 25 ~ 30 DEG C of greenhouse temperature, and relative air humidity 40 ~ 60% obtains after surviving
To dragon fruit plant.
Cotyledonary node is easiest to evoking adventive bud and occurs to be located at the incision being connected with epicotyl, and hypocotyl is easiest to induction not
Determine root growth, under the rational proportion of culture medium of the present invention, adventitious bud and adventitious root can synchronously occur.
Ethylene can induce adventitious root and bud occurs, and break the dormancy of vegetable seeds and bud, promote adventitious bud inducing generation, contracting
Short incubation time；Tomato puree is rich in vitamin A, trace element, protein and glucide, not only provides callus growth institute
The nutrition needed, while vitamin A can promote cell growth.
Charcoal has porous structure, can reduce nitrogen volatilization, reduces nutrition leak；Charcoal can improve cation exchange
Ability, so as to improve the activity of culture medium, dragon fruit root system can in multiporous biological charcoal faster stronger growth, porous structure
Stronger oxonium ion exchange capacity is improved, promotes root respiration, so as to improve plant metabolism ability, improves plant
The resistance of strain.
The basic element of cell division has hypotoxicity, and the present invention promotes cell to increase, reduces use using the vitamin a in tomato
The basic element of cell division is potentially dangerous；Early period, tissue cultures promoted the chloroplaset amount and root growth of dragon fruit plant, improved
Dragon fruit plant adapts to the ability of environment, shortens the hardening time, so as to shorten whole incubation time.
Using the property control callus of charcoal to the constant absorption of culture medium nutrient, humic acid and rare earth element promote
It is absorbed into root system and the synthesis of chloroplaset, charcoal porous structure provides more dissolved oxygens so as to promote respiratory intensity, carry significantly
The high growth efficiency of plant, shortens cultivation cycle.
WPM (Woody Plant medium) is xylophyta culture medium, can be commercially available on the market.
As a further improvement on the present invention, the humic acid molecular weight is 8000 ~ 9000D.
Macromolecule (>10000D) and small-molecular-weight (<Humic acid 9000D) is to the facilitation effect of plant absorption nitrate
It is better than the humic acid of intermediate molecular weight (9000 ~ 10000D), lower molecular weight components can significantly stimulate root system of plant to nitric acid
The absorption of radical ion；Humic acid can promote absorption of the tissue to earth solution middle rare earth nitrate compound, so as to promote to grow.
As a further improvement on the present invention, the earth solution containing cerous nitrate 11.5 ~ 14.0%, lanthanum nitrate 6.0 ~ 8.0%,
Neodymium nitrate 3.0 ~ 5.0%.
Rare-earth element cerium, lanthanum, neodymium can promote chloroplaset to synthesize, and improve respiration, three's synergistic effect, it is indefinite to shorten
The growth time of bud, while the respiratory intensity of callus is improved, make plant strain growth power obtained by the later stage strong.
As a further improvement on the present invention, the charcoal is one in maize straw charcoal, corncob charcoal, peanut shell charcoal
Kind.
Maize straw, corncob and the peanut shell micro pore volume after charing are multiplied, and microcellular structure enriches, and fixed carbon contains
Amount greatly improves, and cation exchange capacity (CEC) increases, and can effectively adjust the balance of nutriment in culture medium, reduces nutrient loss, promotees
It is absorbed into root system.
As a further improvement on the present invention, the tomato puree production method is：Clean disease-free tomato is taken, in squeezing the juice
It squeezes the juice in machine, moisture 40% ~ 60% is concentrated into after 20 ~ 30min of high pressure sterilization, you can obtain tomato puree.
The excessively high form for influencing culture medium of water content, callus are unfavorable for growing in liquid medium, respiration
It reduces, causes germination percentage and rooting rate low.
As a further improvement on the present invention, the hardening was prior to 50 ~ 75% lower refining seedling of shading rate 1 ~ 2 day, then at shading
90 ~ 95% lower refining seedling of rate 3 ~ 4 days, hardening terminates.
Beneficial effects of the present invention：Tissue cultures are carried out using the dragon fruit cotyledonary node for being easy to bud and root induction, with tomato
Mud promotes plant cell growth as nutriment, and green natural reduces the amount using the basic element of cell division；Rare earth element, corruption
It grows acid and charcoal mutually acts synergistically, promote the growing amount of chloroplaset, improve absorption of the root system to nutrition, charcoal can have
The burst size of effect control nutriment improves the utilization rate of culture medium so that adventitious bud is synchronous with adventitious root to be occurred, and is shortened
The cultivation cycle of plant, reduces cost.
Specific embodiment
With reference to embodiment, the present invention is further described.
Embodiment 1
（1）The processing of seedling tissue
Dragon fruit edible tender branch is taken to clean up, with 75% concentration ethanol postincubation 30s, aseptic water washing 1 time cuts off seedling and portion
Hypocotyl is divided to obtain 3cm cotyledonary nodes, removes marrow, then the Tween-40 sterilizing 15min of the NaClO and 0.1% with 10%, sterile water
It rinses 4 times, the cotyledonary node after sterilizing is cut into width as 2cm, the small pieces of a length of 3cm, obtains cotyledon nodal plate；
（2）Adventitious bud, root induction
The cotyledon nodal plate handled well is inoculated into inducing culture, is 25 DEG C, intensity of illumination 1500lux in temperature, during illumination
Between cultivate under 16h/d, obtain aseptic seedlings；
The inducing culture is WPM+KT 1.0mg/L+NAA 0.4mg/L+ethylene 2.0mg/L+tomato puree
35g/L+agar 6g/L+earth solution 5ml/L+charcoal 100g/L+humic acid 10mg/L, pH 6.0；It is described dilute
Native solution contains cerous nitrate 11.5%, lanthanum nitrate 6.0%, neodymium nitrate 3.0%；The humic acid molecular weight is 8000D；The biology
Charcoal is maize straw charcoal；The tomato puree production method is：Clean disease-free tomato is taken, is squeezed the juice in juice extractor, high pressure is gone out
Moisture 40% is concentrated into after bacterium 20min, you can obtain tomato puree；
（3）When adventitious bud and adventitious root grow to 3cm, bottle seedling is moved in the greenhouse of outdoor ventilation, bottle stopper is opened, prior to shading
50% lower refining seedling of rate 2 days, then at 90% lower refining seedling of shading rate 4 days, when bottle seedling grows 2 thorn seats, hardening terminated, and obtains nascent plant
Strain；
（4）After nascent plant is washed with water removal culture medium, plant in charcoal：Perlite=2:In 1 matrix, planting
It is preceding that matrix is sprinkled profoundly water in advance, a water is poured within 3 days, keeps 25 DEG C of greenhouse temperature, relative air humidity 40% obtains fire after surviving
Imperial fruit plant.
Embodiment 2
（1）The processing of seedling tissue
Dragon fruit edible tender branch is taken to clean up, with 75% concentration ethanol postincubation 60s, aseptic water washing 2 times cuts off seedling and portion
Hypocotyl is divided to obtain 4cm cotyledonary nodes, removes marrow, then the Tween-40 sterilizing 20min of the NaClO and 0.5% with 20%, sterile water
It rinses 5 times, the cotyledonary node after sterilizing is cut into width as 3cm, the small pieces of a length of 4cm, obtains cotyledon nodal plate；
（2）Adventitious bud, root induction
The cotyledon nodal plate handled well is inoculated into inducing culture, is 30 DEG C, intensity of illumination 3000lux in temperature, during illumination
Between cultivate under 14h/d, obtain aseptic seedlings；
The inducing culture is WPM+KT 3.0mg/L+NAA 1.0mg/L+ethylene 4.0mg/L+tomato puree
45g/L+agar 8g/L+earth solution 15ml/L+charcoal 120g/L+humic acid 20mg/L, pH 6.5；It is described
Earth solution contains cerous nitrate 14.0%, lanthanum nitrate 8.0%, neodymium nitrate 5.0%；The humic acid molecular weight is 9000D；The life
Object charcoal is corncob charcoal；The tomato puree production method is：Clean disease-free tomato is taken, is squeezed the juice in juice extractor, high pressure is gone out
Moisture 60% is concentrated into after 20 ~ 30min of bacterium, you can obtain tomato puree；
（3）When adventitious bud and adventitious root grow to 5cm, bottle seedling is moved in the greenhouse of outdoor ventilation, bottle stopper is opened, prior to shading
75% lower refining seedling of rate 3 days, then at 95% lower refining seedling of shading rate 5 days, when bottle seedling grows 3 thorn seats, hardening terminated, and obtains nascent plant
Strain；
（4）After nascent plant is washed with water removal culture medium, plant in charcoal：Perlite=2:In 1 matrix, planting
It is preceding that matrix is sprinkled profoundly water in advance, a water is poured within 5 days, keeps 30 DEG C of greenhouse temperature, relative air humidity 60% obtains fire after surviving
Imperial fruit plant.
Embodiment 3
（1）The processing of seedling tissue
Dragon fruit edible tender branch is taken to clean up, with 75% concentration ethanol postincubation 40s, aseptic water washing 2 times cuts off seedling and portion
Hypocotyl is divided to obtain 4cm cotyledonary nodes, removes marrow, then the Tween-40 sterilizing 20min of the NaClO and 0.3% with 15%, sterile water
It rinses 5 times, the cotyledonary node after sterilizing is cut into width as 3cm, the small pieces of a length of 4cm, obtains cotyledon nodal plate；
（2）Adventitious bud, root induction
The cotyledon nodal plate handled well is inoculated into inducing culture, is 28 DEG C, intensity of illumination 2000lux in temperature, during illumination
Between cultivate under 15h/d, obtain aseptic seedlings；
The inducing culture is WPM+KT 2.0mg/L+NAA 0.8mg/L+ethylene 3.0mg/L+tomato puree
40g/L+agar 7g/L+earth solution 10ml/L+charcoal 110g/L+humic acid 15mg/L, pH 6.2；It is described
Earth solution is containing cerous nitrate 13.5%, lanthanum nitrate 7.4%, neodymium nitrate 4.0%；The humic acid molecular weight is 8500D；The biology
Charcoal is peanut shell charcoal；The tomato puree production method is：Clean disease-free tomato is taken, is squeezed the juice in juice extractor, high pressure sterilization
Moisture 50% is concentrated into after 20 ~ 30min, you can obtain tomato puree；
（3）When adventitious bud and adventitious root grow to 4cm, bottle seedling is moved in the greenhouse of outdoor ventilation, bottle stopper is opened, prior to shading
60% lower refining seedling of rate 2 days, then at 95% lower refining seedling of shading rate 4 days, when bottle seedling grows 3 thorn seats, hardening terminated, and obtains nascent plant
Strain；
（4）After nascent plant is washed with water removal culture medium, plant in charcoal：Perlite=2:In 1 matrix, planting
It is preceding that matrix is sprinkled profoundly water in advance, a water is poured within 4 days, keeps 28 DEG C of greenhouse temperature, relative air humidity 50% obtains fire after surviving
Imperial fruit plant.
1 ~ 3 dragon fruit plant strain growth situation such as table 1 of embodiment.
1 embodiment of table, 1 ~ 3 dragon fruit seedling effect
Above example is only exemplary embodiment of the present invention, is not used in the limitation present invention, protection scope of the present invention is by weighing
Sharp claim limits.Those skilled in the art can the present invention essence and protection domain in, the present invention is made various modifications or
Equivalent substitution, this modification or equivalent substitution also should be regarded as being within the scope of the present invention.
Claims (6)
1. a kind of synchronous cultural method of dragon fruit plant, which is characterized in that comprise the following steps：
A., the dragon fruit cotyledonary node of no disease and pests harm in bottle is synchronously carried out to adventitious bud inducing and is taken root, obtains aseptic seedlings；
B. aseptic seedling bottle stopper is opened, by aseptic seedlings ventilation and is interrupted illumination cultivation, obtains nascent plant；
C. after nascent plant being washed away culture medium with clear water, transplant into matrix, sprinkle profoundly water and be managed culture, both obtain dragon fruit
Plant；
The step A is specific as follows：
（1）The processing of seedling tissue
Dragon fruit edible tender branch is taken to clean up, with 75% concentration 30 ~ 60s of ethanol postincubation, aseptic water washing 1 ~ 2 time cuts off seedling
3 ~ 4cm cotyledonary nodes are obtained with part hypocotyl, marrow is removed, then is gone out with 10% ~ 20% NaClO and 0.1% ~ 0.5% Tween-40
Cotyledonary node after sterilizing is cut into width as 2cm ~ 3cm, the small pieces of a length of 3cm ~ 4cm by 15 ~ 20min of bacterium, aseptic water washing 4 ~ 5 times,
Obtain cotyledon nodal plate；
（2）The induction of adventitious bud, root
The cotyledon nodal plate handled well is inoculated into inducing culture, is 25 ~ 30 DEG C in temperature, intensity of illumination for 1500 ~
It is cultivated under 3000lux, 14 ~ 16h/d of light application time, obtains aseptic seedlings；
The inducing culture is 0.4 ~ 1.0mg/L of WPM+KT 1.0 ~ 3.0mg/L+NAA+2.0 ~ 4.0mg/ of ethylene
L+35 ~ 45g/L of tomato puree+6 ~ 8g/L of agar+5 ~ 15ml/L of earth solution+100 ~ 120g/L of charcoal+humic
Acid 10 ~ 20mg/L, pH are 6.0 ~ 6.5；
The step B is specific as follows：
When adventitious bud and adventitious root grow to 3 ~ 5cm, bottle seedling is moved in the greenhouse of outdoor ventilation, opens bottle stopper, hardening 4 ~ 6
My god, when bottle seedling grows 2 ~ 3 thorn seats, stop hardening, obtain nascent plant；
The step C is specific as follows：
After nascent plant is washed with water removal culture medium, plant in charcoal：Perlite=2:It is right before planting in 1 matrix
Matrix sprinkles profoundly water in advance, pours within 3 ~ 5 days a water, keeps 25 ~ 30 DEG C of greenhouse temperature, and relative air humidity 40 ~ 60% obtains after surviving
To dragon fruit plant.
A kind of 2. synchronous cultural method of dragon fruit plant according to claim 1, which is characterized in that the humic acid point
Son amount is 8000 ~ 9000D.
A kind of 3. synchronous cultural method of dragon fruit plant according to claim 1, which is characterized in that the earth solution
Containing cerous nitrate 11.5 ~ 14.0%, lanthanum nitrate 6.0 ~ 8.0%, neodymium nitrate 3.0 ~ 5.0%.
4. the synchronous cultural method of a kind of dragon fruit plant according to claim 1, which is characterized in that the charcoal is
One kind in maize straw charcoal, corncob charcoal, peanut shell charcoal.
5. the synchronous cultural method of a kind of dragon fruit plant according to claim 1, which is characterized in that the tomato is clay
It is as method：Clean disease-free tomato is taken, squeezes the juice in juice extractor, moisture is concentrated into after 20 ~ 30min of high pressure sterilization
40% ~ 60%, you can obtain tomato puree.
6. the synchronous cultural method of a kind of dragon fruit plant according to claim 1, which is characterized in that the hardening is first
In 50 ~ 75% lower refining seedling of shading rate 1 ~ 2 day, then at 90 ~ 95% lower refining seedling of shading rate 3 ~ 4 days, hardening terminated.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108770695A (en) * | 2018-09-10 | 2018-11-09 | 西南林业大学 | A kind of method of the in vitro tissue culture sprouting and rooting of red heart dragon fruit |
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2017
- 2017-12-28 CN CN201711461482.0A patent/CN108040880A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108770695A (en) * | 2018-09-10 | 2018-11-09 | 西南林业大学 | A kind of method of the in vitro tissue culture sprouting and rooting of red heart dragon fruit |
| CN108770695B (en) * | 2018-09-10 | 2022-01-21 | 西南林业大学 | Method for in-vitro tissue culture and rapid seedling establishment of red-heart pitaya |
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Application publication date: 20180518 |