CN108770695A - A kind of method of the in vitro tissue culture sprouting and rooting of red heart dragon fruit - Google Patents
A kind of method of the in vitro tissue culture sprouting and rooting of red heart dragon fruit Download PDFInfo
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- CN108770695A CN108770695A CN201811051691.2A CN201811051691A CN108770695A CN 108770695 A CN108770695 A CN 108770695A CN 201811051691 A CN201811051691 A CN 201811051691A CN 108770695 A CN108770695 A CN 108770695A
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- 244000157072 Hylocereus undatus Species 0.000 title claims abstract description 50
- 235000018481 Hylocereus undatus Nutrition 0.000 title claims abstract description 50
- 235000001808 Ceanothus spinosus Nutrition 0.000 title claims abstract description 38
- 241001264786 Ceanothus spinosus Species 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000000338 in vitro Methods 0.000 title claims abstract description 14
- 230000004069 differentiation Effects 0.000 claims abstract description 6
- 239000000835 fiber Substances 0.000 claims abstract description 6
- 238000005286 illumination Methods 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000001963 growth medium Substances 0.000 claims description 13
- 229920001817 Agar Polymers 0.000 claims description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 12
- 239000008272 agar Substances 0.000 claims description 12
- 239000005720 sucrose Substances 0.000 claims description 12
- 230000001939 inductive effect Effects 0.000 claims description 10
- 230000006698 induction Effects 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000003599 detergent Substances 0.000 claims description 4
- 229910001710 laterite Inorganic materials 0.000 claims description 4
- 239000011504 laterite Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000011159 matrix material Substances 0.000 claims description 4
- 229960002523 mercuric chloride Drugs 0.000 claims description 4
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 4
- 230000035755 proliferation Effects 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 239000008223 sterile water Substances 0.000 claims description 4
- 229910052902 vermiculite Inorganic materials 0.000 claims description 4
- 239000010455 vermiculite Substances 0.000 claims description 4
- 235000019354 vermiculite Nutrition 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 10
- 238000005516 engineering process Methods 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 abstract description 3
- 230000000249 desinfective effect Effects 0.000 abstract description 3
- 229930192334 Auxin Natural products 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 229930014669 anthocyanidin Natural products 0.000 description 2
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 2
- 235000008758 anthocyanidins Nutrition 0.000 description 2
- 239000002363 auxin Substances 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 239000002985 plastic film Substances 0.000 description 2
- 229920006255 plastic film Polymers 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical group [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical group [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000011574 phosphorus Chemical group 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000011782 vitamin Chemical group 0.000 description 1
- 229930003231 vitamin Chemical group 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical group 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to technical field of plant propagation, more particularly to a kind of method of the in vitro tissue culture sprouting and rooting of red heart dragon fruit, this method takes out spinule using the stem section of the barbed seat of red heart dragon fruit as explant after disinfecting, carry out Fiber differentiation, Multiplying culture, culture of rootage and transplanting culture respectively.The improved thorn seat submergence of the present invention can significantly speed up germinating and the quantity of budlet in the medium; and in budlet process of rooting culture; the nascent i.e. transplanting culture of adventitious root; significantly reduce red heart dragon fruit seeling industry period, 3-5 times of growth coefficient, rooting rate 100%; 96% or more survival rate after transplanting; seeling industry period about 90d is a kind of technology that red heart dragon fruit is quickly bred, and is suitable for seedling industrialized red heart dragon fruit kind, scale and intensive manufacture.
Description
Technical field
The invention belongs to technical field of plant propagation, and in particular to a kind of side of the in vitro tissue culture sprouting and rooting of red heart dragon fruit
Method.
Background technology
Red heart dragon fruit is also known as red dragon fruit, is dragon fruit（Hylocereus undatus）A kind of novel have
The fruit of good health-care efficacy, pulp contain a large amount of natural anthocyanidin and show red.The fruits nutrition of red heart dragon fruit is abundant,
Containing the minerals such as abundant calcium, phosphorus, iron and vitamin group, being in addition also rich in has phytalbumin, anthocyanidin and a large amount
Water-soluble dietary fiber, sugariness are far above common dragon fruit, change over the common belief of " dragon fruit is good-looking not to be very good eating ", depth
Liked by people, in the extensive introducing and planting in China tropical and subtropical region, seedling demand is big, and supply falls short of demand for seedling.
Mainly by modes such as seed, cuttage, graftings, the reproduction speed of these methods is slower for the breeding of dragon fruit, and by
The limitation of propagating materials supply, it is difficult to meet the needs of market.In recent years, also there is researcher in the tissue cultures side of dragon fruit
Face is studied, and is made some progress." one kind carries the patent of invention for being 201410135613.6 such as number of patent application
The method of high dragon fruit tissue-cultured seedling planting percent " discloses a kind of method improving dragon fruit tissue-cultured seedling planting percent, wherein from explant
Germinate budlet 35-40d, seedling period is needed about to need 100d at body thorn seat.The hair that number of patent application is 201510297283.5
A kind of bright patent " dragon fruit tissue culture and rapid propagation method ", discloses a kind of dragon fruit tissue culture and rapid propagation method, wherein being lured by explant
The time for exporting budlet is 28-32d, and the seedling period is more than 100d.Same species different genotype is to group culturation rapid propagating technology system
Requirement it is different.The above method is the tissue culture method of common dragon fruit, these methods are in the tissue culture application of red heart dragon fruit
It is ineffective.
A set of rapid propagation in vitro system stable, efficient about red heart dragon fruit is also lacked at present.Meanwhile it can further contract
The incubation time of links and ensure its quality in short red heart dragon fruit tissue culture, the production of red heart dragon fruit tissue culture will be directly affected
It can realize the target of factorial praluction.
Invention content
It is an object of the invention to：In view of the above-mentioned problems, providing a kind of side of the in vitro tissue culture sprouting and rooting of red heart dragon fruit
Method, for red heart dragon fruit kind is seedling industrialized, scale and intensive manufacture provide technical support.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of method of the in vitro tissue culture sprouting and rooting of red heart dragon fruit, it is characterised in that：With the stem section of the barbed seat of red heart dragon fruit
For explant, spinule is taken out after disinfecting, and carries out Fiber differentiation, Multiplying culture, culture of rootage and transplanting culture respectively.
The sterilization method is：Explant is impregnated using liquid detergent solution and scrubs thorn portions position with hairbrush, then uses clear water
Rinse 30min；Explant is immersed in 10s in 75% alcohol later, aseptic water washing is used after taking-up 2 times, then explant is put into
Disinfect 5-8min in 0.1% mercuric chloride solution, is rinsed 6-8 times repeatedly with sterile water after taking-up.
The method of the Fiber differentiation is：The explant for sterilizing and pull out prickle is inoculated on inducing culture vertically,
It is placed in intensity of illumination 1600-1800Lux, illumination 12h/ days, temperature is illumination cultivation 10-15d under conditions of 25 ± 2 DEG C, is obtained
Germinate the budlet for high 2-3cm or so in explant thorn portions position.
Wherein, it is explant to be inserted vertically into culture medium, and pierce seat that the explant, which is inoculated in vertically on inducing culture,
Position is immersed in culture medium.The inducing culture be 1.0 ~ 1.5 mg/L of MS 2.0 ~ 3.0mg/L of+6-BA+NAA+
20 ~ 30g/L of sucrose+4.0 ~ 6.0g/L of agar, pH value are 5.8 ~ 6.2.
The inducing culture of the present invention, and the mode that explant is inoculated in vertically on inducing culture is taken to lure
It leads, and can significantly shorten budlet eruption number of days compared with prior art, inventor takes tradition with regard to published inducing culture
The mode that is located on culture medium of thorn portions position carry out contrast test, as a result such as following table：
。
The method of the Multiplying culture is：By the budlet of induction germinating after explant is cut, it is inoculated in proliferated culture medium
On, it is placed in intensity of illumination 1600-1800Lux, illumination 12h/ days, temperature is illumination cultivation 25-30d under conditions of 25 ± 2 DEG C, from
Germinate the Multiple Buds for 3-5 high about 4-5cm at budlet base portion.Wherein, the proliferated culture medium be MS+6-BA 1.0 ~
0.5 ~ 1.0mg/L of 2.0mg/L+NAA+20 ~ 30g/L of sucrose+4.0 ~ 7.0g/L of agar, pH value are 5.8 ~ 6.2.
Compared with published Multiplying culture based component, it is as follows that proliferated culture medium of the invention has difference：
。
The basic element of cell division 6-BA of low concentration is combined by the proliferated culture medium that the present invention uses with auxins NAA, production
Significant synergistic function is given birth to, not only growth coefficient reaches 3-5 times, but also proliferation bud growing way is vigorous, highly reaches 4-
5cm not can be used to rooting induction culture by strong seedling culture link, shorten the red heart dragon fruit tissue-cultured seedling production cycle,
Production cost is saved.
The method of the culture of rootage is：The Multiple Buds that Multiplying culture obtains are cut into simple bud, are transferred in root media
On, it is placed in intensity of illumination 1600-1800Lux, illumination 12h/ days, temperature is illumination cultivation 10-15d under conditions of 25 ± 2 DEG C, is waited for
Simple bud base portion has 2-3 root adventitious roots to germinate to be transplanted to seedling culture hole plate and carry out transplanting culture.
Wherein, the root media is 0.2 ~ 1.0 0.1 ~ 0.5mg/L of mg/L+IAA of 1/2MS+IBA+sucrose
20 ~ 30g/L+4.0 ~ 7.0g/L of agar, pH value 5.8-6.2.
The present invention is compared with the root induction medium component for disclosing invention：
。
The 1/2MS of lower salt content of the present invention and the 2 of low concentration kinds of auxins exogenous hormone proportionings use, and have played more
Apparent synergistic function can not only promote inoculation budlet that can germinate in its base portion in 10-15d and 2-3 root adventitious roots,
Rooting rate reaches 100%, and budlet robust growth, can be used for transplanting seedling culture, shortens production cycle 15d compared with prior art
More than.
It is described transplanting culture method be：By seedling replanting in being filled with laterite：Fertile soil：Vermiculite=1:1:1 matrix is educated
Seedling hole tray is watered with water, seedling culture hole plate is positioned in the double-deck arched shed in greenhouse and cultivates 30d, obtains high 8- per 1 seedling of cave
The sturdy red heart dragon fruit seedling of 12cm, radical 6-8 roots, root long 10-12cm, root system.
Wherein, the double-deck arched shed in the greenhouse is the Small plastic shed for building high 50-60cm first, covers one layer above
White plastic film, and then the arched shed of high 100cm is built in Small plastic shed outer layer, one layer of White plastic film is covered above.
The present invention by the way that using the stem section of the barbed seat of red heart dragon fruit as explant, spinule is taken out after disinfecting, respectively into
The small bud inducement cultivation of row, Multiplying culture, culture of rootage and transplanting culture and etc. establish red heart dragon fruit stability and high efficiency from
The technical system of body tissue culture sprouting and rooting.Wherein, step（2）It is middle that explant thorn portions position is immersed in culture medium, it can compare
The prior art shortens budlet induction time 15d or so；Step（3）Multiplying culture realizes 3-5 times of growth coefficient；Step（4）It is raw
It when root culture, waits for that budlet base portion tentatively germinates and 2-3 root adventitious roots, that is, carry out transplanting culture, when shortening culture compared with the prior art
Between 15d or so, and rooting rate reaches 100%；Step（5）The survival rate of culture is transplanted 96% or more.The integrated application invention skill
Art can obtain the nursery stock for meeting production requirement in 90d, and can realize 1,000,000 plants of nursery stock of year production or more.
To sum up, the in vitro tissue culture sprouting and rooting method of red heart dragon fruit of the invention is that a kind of red heart dragon fruit is quickly bred
Technology is suitable for seedling industrialized red heart dragon fruit kind, scale and intensive manufacture.
Specific implementation mode
Below by way of specific embodiment, the invention will be further described.
Embodiment 1:The method of the in vitro tissue culture sprouting and rooting of red heart dragon fruit disclosed in the present embodiment, includes the following steps：
（1）Explant chooses and disinfection：The red heart dragon fruit stem section with spinosity seat is taken, impregnates explant using liquid detergent solution
Hairbrush scrub thorn portions position is used in combination, then 30min is rinsed with clear water；Explant is immersed in 10s in 75% alcohol later, after taking-up
It is put into 0.1% mercuric chloride solution the 5-8min that disinfects with aseptic water washing 2 times, then by explant, is rinsed repeatedly with sterile water after taking-up
6-8 times, prickle is taken out with the son of taking the photograph after disinfection；
（2）Fiber differentiation：The explant for sterilizing and pull out prickle is inoculated in MS+6-BA 2.0mg/L+NAA 1.5 vertically
Mg/L+sucrose 20g/L+agar 6.0g/L, on the inducing culture that pH value is 5.8 ~ 6.2, and the portions positions leaching of explant thorn
Not in culture, it is placed in intensity of illumination 1600-1800Lux, illumination 12h/ days, temperature cultivates 10- under conditions of being 25 ± 2 DEG C
15d obtains explant thorn portions position and germinates the budlet for high 2-3cm or so；
（3）Multiplying culture：By the budlet of induction germinating after explant is cut, it is inoculated in MS+6-BA 1.0mg/L+NAA
1.0mg/L+sucrose 30g/L+agar 4.0g/L, pH value are placed in illumination to carry out Multiplying culture on 5.8 ~ 6.2 culture medium
Intensity 1600-1800Lux, illumination 12h/ days, temperature is illumination cultivation 25-30d under conditions of 25 ± 2 DEG C, from budlet base portion
Germinate the Multiple Buds for 3-5 high about 4-5cm；
（4）Culture of rootage：The Multiple Buds that Multiplying culture obtains are cut into simple bud, are transferred in 0.5 mg/L+IAA of 1/2MS+IBA
0.1mg/L+sucrose 20g/L+agar 5g/L, pH value are to be placed in intensity of illumination 1600- on the root media of 5.8-6.2
1800Lux, illumination 12h/ days, temperature are illumination cultivation 10-15d under conditions of 25 ± 2 DEG C, wait for that simple bud base portion has 2-3 roots indefinite
Root, which germinates, to be transplanted to seedling culture hole plate and carries out transplanting culture；
（5）Transplanting culture：By seedling replanting in being filled with laterite：Fertile soil：Vermiculite=1:1:The seedling culture hole plate of 1 matrix, per cave 1
Seedling is watered with water, and seedling culture hole plate is positioned in the double-deck arched shed in greenhouse and cultivates 30d and can nursery stock be changed bag and be out of the garden.
The fast-propagation of red heart dragon fruit is carried out using the method, average coefficient of proliferation up to 4.6 times, rooting rate up to 100%,
Survival rate 98.6% is cultivated in average transplanting, and average height of seedling 10.6cm, average lateral root number 8, average root long are can get in 90d
The sturdy red heart dragon fruit seedling of 12cm, root system.
Embodiment 2:The method of the in vitro tissue culture sprouting and rooting of red heart dragon fruit disclosed in the present embodiment, includes the following steps：
（1）Explant chooses and disinfection：The red heart dragon fruit stem section with spinosity seat is taken, impregnates explant using liquid detergent solution
Hairbrush scrub thorn portions position is used in combination, then 30min is rinsed with clear water；Explant is immersed in 10s in 75% alcohol later, after taking-up
It is put into 0.1% mercuric chloride solution the 5-8min that disinfects with aseptic water washing 2 times, then by explant, is rinsed repeatedly with sterile water after taking-up
6-8 times, prickle is taken out with the son of taking the photograph after disinfection；
（2）Fiber differentiation：The explant for sterilizing and pull out prickle is inoculated in MS+6-BA 3.0mg/L+NAA 1.0 vertically
Mg/L+sucrose 30g/L+agar 4.0g/L, on the inducing culture that pH value is 5.8 ~ 6.2, and the portions positions leaching of explant thorn
Not in culture, it is placed in intensity of illumination 1600-1800Lux, illumination 12h/ days, temperature cultivates 10- under conditions of being 25 ± 2 DEG C
15d obtains explant thorn portions position and germinates the budlet for high 2-3cm or so；
（3）Multiplying culture：By the budlet of induction germinating after explant is cut, it is inoculated in MS+6-BA 2.0mg/L+NAA
0.5mg/L+sucrose 30g/L+agar 7.0g/L, pH value are placed in illumination to carry out Multiplying culture on 5.8 ~ 6.2 culture medium
Intensity 1600-1800Lux, illumination 12h/ days, temperature is illumination cultivation 25-30d under conditions of 25 ± 2 DEG C, from budlet base portion
Germinate the Multiple Buds for 3-5 high about 4-5cm；
（4）Culture of rootage：The Multiple Buds that Multiplying culture obtains are cut into simple bud, are transferred in 1.0 mg/L+IAA of 1/2MS+IBA
0.5mg/L+sucrose 30g/L+agar 6g/L, pH value are to be placed in intensity of illumination 1600- on the root media of 5.8-6.2
1800Lux, illumination 12h/ days, temperature are illumination cultivation 10-15d under conditions of 25 ± 2 DEG C, wait for that simple bud base portion has 2-3 roots indefinite
Root, which germinates, to be transplanted to seedling culture hole plate and carries out transplanting culture；
（5）Transplanting culture：By seedling replanting in being filled with laterite：Fertile soil：Vermiculite=1:1:The seedling culture hole plate of 1 matrix, per cave 1
Seedling is watered with water, and seedling culture hole plate is positioned in the double-deck arched shed in greenhouse and cultivates 30d and can nursery stock be changed bag and be out of the garden.
The fast-propagation of red heart dragon fruit is carried out using the method, average coefficient of proliferation up to 5 times, up to 100% put down by rooting rate
Survival rate 96.7% is cultivated in transplanting, and average height of seedling 11.8cm, average lateral root number 6.9, average root long are can get in 90d
The sturdy red heart dragon fruit seedling of 11.6cm, root system.
Claims (4)
1. a kind of method of the in vitro tissue culture sprouting and rooting of red heart dragon fruit, specifically comprises the following steps：
（1）Explant chooses and disinfection：The red heart dragon fruit stem section with spinosity seat is taken, impregnates explant using liquid detergent solution
Hairbrush scrub thorn portions position is used in combination, then 30min is rinsed with clear water；Explant is immersed in 10s in 75% alcohol later, after taking-up
It is put into 0.1% mercuric chloride solution the 5-8min that disinfects with aseptic water washing 2 times, then by explant, is rinsed repeatedly with sterile water after taking-up
6-8 times, prickle is taken out with the son of taking the photograph after disinfection；
（2）Fiber differentiation：The explant for sterilizing and pull out prickle is inoculated on inducing culture vertically, and explant pierces portions
Position is immersed in culture, is placed in intensity of illumination 1600-1800Lux, illumination 12h/ days, and temperature is cultivated under conditions of being 25 ± 2 DEG C
10-15d obtains explant thorn portions position and germinates the budlet for high 2-3cm or so；
（3）Multiplying culture：By the budlet of induction germinating after explant is cut, it is inoculated on proliferated culture medium and carries out proliferation training
It supports, is placed in intensity of illumination 1600-1800Lux, illumination 12h/ days, temperature is illumination cultivation 25-30d under conditions of 25 ± 2 DEG C, from
Germinate the Multiple Buds for 3-5 high about 4-5cm at budlet base portion；
（4）Culture of rootage：The Multiple Buds that Multiplying culture obtains are cut into simple bud, transfers on root media, it is strong to be placed in illumination
1600-1800Lux, illumination 12h/ days are spent, temperature is illumination cultivation 10-15d under conditions of 25 ± 2 DEG C, waits for that simple bud base portion has 2-3
Root adventitious root, which germinates, to be transplanted to seedling culture hole plate and carries out transplanting culture；
（5）Transplanting culture：By seedling replanting in being filled with laterite：Fertile soil：Vermiculite=1:1:The seedling culture hole plate of 1 matrix, per cave 1
Seedling is watered with water, and seedling culture hole plate is positioned in the double-deck arched shed in greenhouse and cultivates 30d and can nursery stock be changed bag and be out of the garden.
2. the method for the in vitro tissue culture sprouting and rooting of red heart dragon fruit as described in claim 1, it is characterised in that inducing culture
Using 1.0 ~ 1.5 mg/L of MS+6-BA 2.0 ~ 3.0mg/L+NAA+20 ~ 30g/L of sucrose+4.0 ~ 6.0g/L of agar,
PH value is 5.8 ~ 6.2.
3. the method for the in vitro tissue culture sprouting and rooting of red heart dragon fruit as described in claim 1, it is characterised in that proliferated culture medium
Using 0.5 ~ 1.0mg/L of MS+6-BA 1.0 ~ 2.0mg/L+NAA+20 ~ 30g/L of sucrose+4.0 ~ 7.0g/L of agar,
PH value is 5.8 ~ 6.2.
4. the method for the in vitro tissue culture sprouting and rooting of red heart dragon fruit as described in claim 1, it is characterised in that proliferated culture medium
For 0.5 ~ 1.0mg/L of MS+6-BA 1.0 ~ 2.0mg/L+NAA+20 ~ 30g/L of sucrose+agar 4.0 ~ 7.0g/L, pH
Value is 5.8 ~ 6.2.
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| CN201811051691.2A CN108770695B (en) | 2018-09-10 | 2018-09-10 | Method for in-vitro tissue culture and rapid seedling establishment of red-heart pitaya |
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| CN201811051691.2A CN108770695B (en) | 2018-09-10 | 2018-09-10 | Method for in-vitro tissue culture and rapid seedling establishment of red-heart pitaya |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111771729A (en) * | 2020-08-27 | 2020-10-16 | 海南瑞民农业科技有限公司 | Tissue culture and rapid propagation method for pitaya |
| CN113170734A (en) * | 2021-06-02 | 2021-07-27 | 中国热带农业科学院橡胶研究所 | Tissue culture, transplantation and domestication method for bird's nest fruit |
| CN113229146A (en) * | 2021-06-02 | 2021-08-10 | 中国热带农业科学院橡胶研究所 | Tissue culture, transplantation and domestication method for red-meat pitaya |
| CN116472959A (en) * | 2023-04-11 | 2023-07-25 | 广西硕果农业开发有限公司 | Culture method for bird's nest fruit seedlings |
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| CN113229146A (en) * | 2021-06-02 | 2021-08-10 | 中国热带农业科学院橡胶研究所 | Tissue culture, transplantation and domestication method for red-meat pitaya |
| CN113170734B (en) * | 2021-06-02 | 2022-11-11 | 中国热带农业科学院橡胶研究所 | Tissue culture, transplantation and domestication method for bird's nest fruit |
| CN116472959A (en) * | 2023-04-11 | 2023-07-25 | 广西硕果农业开发有限公司 | Culture method for bird's nest fruit seedlings |
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