CN108849506A - A kind of method of dragon fruit tissue cultivating and seedling - Google Patents
A kind of method of dragon fruit tissue cultivating and seedling Download PDFInfo
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- CN108849506A CN108849506A CN201810694489.5A CN201810694489A CN108849506A CN 108849506 A CN108849506 A CN 108849506A CN 201810694489 A CN201810694489 A CN 201810694489A CN 108849506 A CN108849506 A CN 108849506A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The present invention provides a kind of method of dragon fruit tissue cultivating and seedling, belong to dragon fruit nursery field, it chooses first and acquires tender stem-tip tissue on excellent maternal plant, the spinule on thorn seat and fur removal marrow are peelled off after cleaning, then it carries out disinfection to obtain explant, then explant is continued to break up in induced medium culture sterile bud cultivating in base sterile bud in seedling, seedling is finally developed into root media, then can carry out subsequent nursery after being tamed.Method provided by the invention can promote the cultivation speed of dragon fruit seedling, reduce growing-seedling period, while can guarantee seedling quality, stablize the merit of heredity maternal plant, is suitable for large scale systemization and plants.
Description
【Technical field】
The present invention relates to dragon fruit seedling-raising technique fields, and in particular to a kind of method of dragon fruit tissue cultivating and seedling.
【Background technique】
Dragon fruit is rich in vegetable albumin, anthocyanidin, vitamin and water-soluble dietary fiber, often edible, has
Weight-reducing reduces cholesterol, prevent constipation, colorectal cancer and other effects, the vegetable albumin contained can with the intracorporal heavy metal of people from
Son combines, and is excluded in vitro by excretory system, and plays the role of removing toxic substances.The anthocyanidin contained can protect human body from freedom
The damage of base enhances blood vessel elasticity, helps to prevent a variety of diseases related with free radical.Therefore, dragon fruit plantation industry exists
The country has a vast market foreground and economic benefit.
Currently, dragon fruit mostly uses seed, cuttage and the mode of reproduction of plant division in cultivation, seminal propagation is poor because of its germination
Different big and offspring's shape separation is obvious, and the kind original of cuttage and breeding is few, it is difficult to meet the market demand；To solve the above problems, adopting
It is short with the method for tissue cultures to be cultivated to dragon fruit the not only period, and seedling quality is high.
【Summary of the invention】
Goal of the invention of the invention is：In view of the above problems, a kind of dragon fruit tissue cultivating and seedling is provided
Method, this method can promote the cultivation speed of dragon fruit seedling, reduce growing-seedling period, while can improve seedling quality, stablize and lose
The merit of maternal plant is passed, is suitable for large scale systemization and plants.
To achieve the goals above, the technical solution adopted by the present invention is as follows：
A kind of method of dragon fruit tissue cultivating and seedling, includes the following steps：
S1：Tender stem-tip tissue is acquired from the excellent maternal plant of robust growth, by it in gnotobasis incision at length
It for the segment of 4~5cm, is rinsed well with tap water, after carefully peelling off spinule and the fur removal marrow on thorn seat, then with sterile
Water is rinsed well, then is put in 20~30s of immersion in alcoholic solution, then 10~12min is sterilized with 0.1% mercuric chloride solution, with nothing
It is explant after bacterium water rinses 5 times, then after blotting surface moisture with aseptic filter paper；
S2：Explant described in step S1 is inoculated into 35~42d of induced medium culture and obtains sterile bud, the induction training
Supporting base is+0.08~0.12mg/L of MS culture medium NAA+2~3mg/L 6-BA+0.5~0.7mg/L IAA+35~40g/L sugarcane
Sugar+5~7g/L agar, it is 5.7~5.9 that above-mentioned substance, which is fitted into culture bottle, and adjusts pH value；
S3：Sterile bud described in step S2 is put into seedling culture medium and carries out 25~30d of cultivation and obtains young shoot, the seedling
Culture medium be+0.08~0.10mg/L of MS culture medium NAA+2.0~2.5mg/L 6-BA+30~32g/L sucrose+4.5~
+ 0.03%~0.04% active carbon of 5.2g/L agar；
S4：By young shoot described in step S3 continue cultivate to length be greater than 2cm after, move in root media cultivate 20~
30d obtains seedling, and the root media is 1/2MS culture medium+0.15~0.20mg/L NAA+0.1~0.2mg/L 6-BA+
30~32g/L sucrose+4.5~5.2g/L agar；
S5：Seedling described in step S4 is subjected to rooting culture.
Further, in step sl, the volumetric concentration of the alcoholic solution is 70~75%.
Further, in step S2~S4, the cultivating process, be temperature be 24~26 DEG C, illumination be 2000~
It is carried out under 2600LX, the time of the illumination is 13~14h/ days.
Further, in step s3, the pH value of the seedling culture medium is 5.7~5.9.
Further, in step s 4, the pH value of the root media is 5.8~6.0.
Further, in step s 5, the domestication is to be impregnated by seedling after bottle outlet in culture bottle with carbendazim solution
Cleaning seedling 2~3 times, removes the culture medium of root, is then planted in compost seed plate.
The present invention provides a kind of methods of dragon fruit tissue cultivating and seedling, compared with prior art, have beneficial below
Effect：
The present invention chooses acquire tender stem-tip tissue on excellent maternal plant first, and the differentiation capability of callus is stronger,
Differentiation rate is higher, and the spinule on thorn seat and fur removal marrow are peelled off after cleaning, then carries out disinfection to obtain explant, and sterilize
With sterilization time strict control, it on the one hand can guarantee explant disinfection and sterilizing sufficiently, on the other hand ensure the tissue of explant
Structure is not destroyed, and disinfection and sterilization effect are good, then by explant in induced medium culture sterile bud, by sterile bud at
Seedling is cultivated to be continued to break up in base, and seedling is finally developed into root media, and applicant is led to according to the nutritional requirement of explant
It crosses research to grope to filter out the type and concentration of suitable culture medium, and the temperature and humidity of strict control culture, accelerates seedling
It is formed, induction time can averagely shorten 4-6d, and inductivity is high, will can carry out subsequent nursery after the domestication of gained seedling.
Above-mentioned each technological means all cooperates, mutually promotes, and camps step by step, is all linked with one another, generated total skill
Art effect is significantly larger than the simple adduction of technological means caused by single technological means.
In short, the present invention provides the cultivation speed that a kind of method of dragon fruit tissue cultivating and seedling can promote dragon fruit seedling
Degree reduces growing-seedling period, while can improve seedling quality, stablizes the merit of heredity maternal plant, is suitable for large scale system kind
It plants.
【Specific embodiment】
Below by way of specific embodiment, the invention will be further described.
Embodiment 1
A kind of method of dragon fruit tissue cultivating and seedling, includes the following steps：
S1：Tender stem-tip tissue is acquired from the excellent maternal plant of robust growth, by it in gnotobasis incision at length
It for the segment of 4cm, is rinsed well with tap water, after carefully peelling off spinule and the fur removal marrow on thorn seat, then uses sterile water
It rinses well, then is put in the alcoholic solution that volumetric concentration is 70% and impregnates 20s, then sterilized with 0.1% mercuric chloride solution
10min is explant after aseptic water washing 5 times, then after blotting surface moisture with aseptic filter paper；
S2：Explant described in step S1 is inoculated into induced medium culture 35d and obtains sterile bud, the induced medium
For MS culture medium+0.08mg/L NAA+2mg/L 6-BA+0.5mg/L IAA+35g/L sucrose+5g/L agar, by above-mentioned substance
Being fitted into culture bottle and adjusting pH value is 5.7；
S3：Sterile bud described in step S2 is put into seedling culture medium carry out cultivate 25d obtain young shoot, the seedling culture
Base is+0.03% active carbon of MS culture medium+0.08mg/L NAA+2.0mg/L 6-BA+30g/L sucrose+4.5g/L agar；It is described
The pH value of seedling culture medium is 5.7；
S4：Young shoot described in step S3 is continued to cultivate to length and is obtained greater than cultivation 20d in root media after 2cm, is moved to
To seedling, the root media be 1/2MS culture medium+0.15mg/L NAA+0.1mg/L 6-BA+30g/L sucrose+4.5~
5.2g/L agar；The pH value of the root media is 5.8；
In step S2~S4, the cultivating process is carried out in the case where temperature is 24 DEG C, illumination is 2000LX, the light
According to time be 13h/ days；
S5：Seedling described in step S4 is subjected to rooting culture；It is described domestication be by seedling after bottle outlet in culture bottle,
With carbendazim solution soaking and washing seedling 2~3 times, the culture medium of root is removed, is then planted in compost seed plate i.e.
It can.
Embodiment 2
A kind of method of dragon fruit tissue cultivating and seedling, includes the following steps：
S1：Tender stem-tip tissue is acquired from the excellent maternal plant of robust growth, by it in gnotobasis incision at length
It for the segment of 4.5cm, is rinsed well with tap water, after carefully peelling off spinule and the fur removal marrow on thorn seat, then with sterile
Water is rinsed well, then is put in the alcoholic solution that volumetric concentration is 72% and is impregnated 25s, is then sterilized with 0.1% mercuric chloride solution
11min is explant after aseptic water washing 5 times, then after blotting surface moisture with aseptic filter paper；
S2：Explant described in step S1 is inoculated into induced medium culture 38d and obtains sterile bud, the induced medium
For MS culture medium+0.1mg/L NAA+2.5mg/L 6-BA+0.6mg/L IAA+38g/L sucrose+6g/L agar, by above-mentioned substance
Being fitted into culture bottle and adjusting pH value is 5.8；
S3：Sterile bud described in step S2 is put into seedling culture medium carry out cultivate 28d obtain young shoot, the seedling culture
Base is+0.035 active carbon of MS culture medium+0.09mg/L NAA+2.2mg/L 6-BA+31g/L sucrose+4.8g/L agar；It is described
The pH value of seedling culture medium is 5.8；
S4：Young shoot described in step S3 is continued to cultivate to length and is obtained greater than cultivation 25d in root media after 2cm, is moved to
To seedling, the root media is 1/2MS culture medium+0.18mg/L NAA+0.15mg/L 6-BA+31g/L sucrose+4.6g/
L agar；The pH value of the root media is 5.9；
In step S2~S4, the cultivating process is carried out in the case where temperature is 25 DEG C, illumination is 2200LX, the light
According to time be 13h/ days；
S5：Seedling described in step S4 is subjected to rooting culture；It is described domestication be by seedling after bottle outlet in culture bottle,
With carbendazim solution soaking and washing seedling 2~3 times, the culture medium of root is removed, is then planted in compost seed plate i.e.
It can.
Embodiment 3
A kind of method of dragon fruit tissue cultivating and seedling, includes the following steps：
S1：Tender stem-tip tissue is acquired from the excellent maternal plant of robust growth, by it in gnotobasis incision at length
It for the segment of 5cm, is rinsed well with tap water, after carefully peelling off spinule and the fur removal marrow on thorn seat, then uses sterile water
It rinses well, then is put in the alcoholic solution that volumetric concentration is 75% and impregnates 30s, then sterilized with 0.1% mercuric chloride solution
12min is explant after aseptic water washing 5 times, then after blotting surface moisture with aseptic filter paper；
S2：Explant described in step S1 is inoculated into induced medium culture 42d and obtains sterile bud, the induced medium
For MS culture medium+0.12mg/L NAA+3mg/L 6-BA+0.7mg/L IAA+40g/L sucrose+7g/L agar, by above-mentioned substance
Being fitted into culture bottle and adjusting pH value is 5.9；
S3：Sterile bud described in step S2 is put into seedling culture medium carry out cultivate 30d obtain young shoot, the seedling culture
Base is+0.04% active carbon of MS culture medium+0.10mg/L NAA+2.5mg/L 6-BA+32g/L sucrose+5.2g/L agar；It is described
The pH value of seedling culture medium is 5.9；
S4：Young shoot described in step S3 is continued to cultivate to length and is obtained greater than cultivation 30d in root media after 2cm, is moved to
To seedling, the root media is 1/2MS culture medium+0.20mg/L NAA+0.2mg/L 6-BA+32g/L sucrose+5.2g/L
Agar；The pH value of the root media is 6.0；
In step S2~S4, the cultivating process is carried out in the case where temperature is 26 DEG C, illumination is 2600LX, the light
According to time be 14h/ days；
S5：Seedling described in step S4 is subjected to rooting culture；It is described domestication be by seedling after bottle outlet in culture bottle,
With carbendazim solution soaking and washing seedling 2~3 times, the culture medium of root is removed, is then planted in compost seed plate i.e.
It can.
Comparative example 1
A kind of method of dragon fruit tissue cultivating and seedling, includes the following steps：
S1：Tender stem-tip tissue is acquired from the excellent maternal plant of robust growth, by it in gnotobasis incision at length
It for the segment of 5cm, is rinsed well with tap water, after carefully peelling off spinule and the fur removal marrow on thorn seat, then uses sterile water
It rinses well, then is put in the alcoholic solution that volumetric concentration is 75% and impregnates 30s, after aseptic water washing 5 times, then with sterile filter
Paper is explant after blotting surface moisture；
S2：Explant described in step S1 is inoculated into induced medium culture 42d and obtains sterile bud, the induced medium
For MS culture medium+0.12mg/L NAA+3mg/L 6-BA+0.7mg/L IAA+40g/L sucrose+7g/L agar, by above-mentioned substance
Being fitted into culture bottle and adjusting pH value is 5.9；
S3：Sterile bud described in step S2 is put into seedling culture medium carry out cultivate 30d obtain young shoot, the seedling culture
Base is+0.04% active carbon of MS culture medium+0.10mg/L NAA+2.5mg/L 6-BA+32g/L sucrose+5.2g/L agar；It is described
The pH value of seedling culture medium is 5.9；
S4：Young shoot described in step S3 is continued to cultivate to length and is obtained greater than cultivation 30d in root media after 2cm, is moved to
To seedling, the root media is 1/2MS culture medium+0.20mg/L NAA+0.2mg/L 6-BA+32g/L sucrose+5.2g/L
Agar；The pH value of the root media is 6.0；
In step S2~S4, the cultivating process is carried out in the case where temperature is 26 DEG C, illumination is 2600LX, the light
According to time be 14h/ days；
S5：Seedling described in step S4 is subjected to rooting culture；It is described domestication be by seedling after bottle outlet in culture bottle,
With carbendazim solution soaking and washing seedling 2~3 times, the culture medium of root is removed, is then planted in compost seed plate i.e.
It can.
Comparative example 2
A kind of method of dragon fruit tissue cultivating and seedling, includes the following steps：
S1：Tender stem-tip tissue is acquired from the excellent maternal plant of robust growth, by it in gnotobasis incision at length
It for the segment of 5cm, is rinsed well with tap water, after carefully peelling off spinule and the fur removal marrow on thorn seat, then uses sterile water
It rinses well, then is put in the alcoholic solution that volumetric concentration is 75% and impregnates 30s, then sterilized with 0.1% mercuric chloride solution
12min is explant after aseptic water washing 5 times, then after blotting surface moisture with aseptic filter paper；
S2：Explant described in step S1 is inoculated into induced medium culture 42d and obtains sterile bud, the induced medium
For MS culture medium+0.12mg/L NAA+40g/L sucrose+7g/L agar, above-mentioned substance, which is fitted into adjusting pH value in culture bottle, is
5.9；
S3：Sterile bud described in step S2 is put into seedling culture medium carry out cultivate 30d obtain young shoot, the seedling culture
Base is+0.04% active carbon of MS culture medium+0.10mg/L NAA+32g/L sucrose+5.2g/L agar；The seedling culture medium
PH value is 5.9；
S4：Young shoot described in step S3 is continued to cultivate to length and is obtained greater than cultivation 30d in root media after 2cm, is moved to
To seedling, the root media is 1/2MS culture medium+0.20mg/L NAA+32g/L sucrose+5.2g/L agar；It is described to take root
The pH value of culture medium is 6.0；
In step S2~S4, the cultivating process is carried out in the case where temperature is 26 DEG C, illumination is 2600LX, the light
According to time be 14h/ days；
S5：Seedling described in step S4 is subjected to rooting culture；It is described domestication be by seedling after bottle outlet in culture bottle,
With carbendazim solution soaking and washing seedling 2~3 times, the culture medium of root is removed, is then planted in compost seed plate i.e.
It can.
Comparative example 3
A kind of method of dragon fruit tissue cultivating and seedling, includes the following steps：
S1：Tender stem-tip tissue is acquired from the excellent maternal plant of robust growth, by it in gnotobasis incision at length
It for the segment of 5cm, is rinsed well with tap water, after carefully peelling off spinule and the fur removal marrow on thorn seat, then uses sterile water
It rinses well, then is put in the alcoholic solution that volumetric concentration is 75% and impregnates 30s, then sterilized with 0.1% mercuric chloride solution
12min is explant after aseptic water washing 5 times, then after blotting surface moisture with aseptic filter paper；
S2：Explant described in step S1 is inoculated into induced medium culture 42d and obtains sterile bud, the induced medium
For MS culture medium+0.12mg/L NAA+3mg/L 6-BA+0.7mg/L IAA+40g/L sucrose+7g/L agar, by above-mentioned substance
Being fitted into culture bottle and adjusting pH value is 5.9；
S3：Sterile bud described in step S2 is put into seedling culture medium carry out cultivate 30d obtain young shoot, the seedling culture
Base is+0.04% active carbon of MS culture medium+0.10mg/L NAA+2.5mg/L 6-BA+32g/L sucrose+5.2g/L agar；It is described
The pH value of seedling culture medium is 5.9；
S4：Young shoot described in step S3 is continued to cultivate to length and is obtained greater than cultivation 30d in root media after 2cm, is moved to
To seedling, the root media is 1/2MS culture medium+0.20mg/L NAA+0.2mg/L 6-BA+32g/L sucrose+5.2g/L
Agar；The pH value of the root media is 6.0；
In step S2~S4, the cultivating process is carried out in the case where temperature is 23 DEG C, illumination is 2600LX, the light
According to time be 14h/ days；
S5：Seedling described in step S4 is subjected to rooting culture；It is described domestication be by seedling after bottle outlet in culture bottle,
With carbendazim solution soaking and washing seedling 2~3 times, the culture medium of root is removed, is then planted in compost seed plate i.e.
It can.
Comparative example 4
A kind of method of dragon fruit tissue cultivating and seedling, includes the following steps：
S1：Tender stem-tip tissue is acquired from the excellent maternal plant of robust growth, by it in gnotobasis incision at length
It for the segment of 5cm, is rinsed well with tap water, after carefully peelling off spinule and the fur removal marrow on thorn seat, then uses sterile water
It rinses well, then is put in the alcoholic solution that volumetric concentration is 75% and impregnates 30s, then sterilized with 0.1% mercuric chloride solution
12min is explant after aseptic water washing 5 times, then after blotting surface moisture with aseptic filter paper；
S2：Explant described in step S1 is inoculated into induced medium culture 42d and obtains sterile bud, the induced medium
For MS culture medium+0.12mg/L NAA+3mg/L 6-BA+0.7mg/L IAA+40g/L sucrose+7g/L agar, by above-mentioned substance
Being fitted into culture bottle and adjusting pH value is 5.9；
S3：Sterile bud described in step S2 is put into seedling culture medium carry out cultivate 30d obtain young shoot, the seedling culture
Base is+0.04% active carbon of MS culture medium+0.10mg/L NAA+2.5mg/L 6-BA+32g/L sucrose+5.2g/L agar；It is described
The pH value of seedling culture medium is 5.9；
S4：Young shoot described in step S3 is continued to cultivate to length and is obtained greater than cultivation 30d in root media after 2cm, is moved to
To seedling, the root media is 1/2MS culture medium+0.20mg/L NAA+0.2mg/L 6-BA+32g/L sucrose+5.2g/L
Agar；The pH value of the root media is 6.0；
In step S2~S4, the cultivating process is carried out in the case where temperature is 27 DEG C, illumination is 1500LX, the light
According to time be 14h/ days；
S5：Seedling described in step S4 is subjected to rooting culture；It is described domestication be by seedling after bottle outlet in culture bottle,
With carbendazim solution soaking and washing seedling 2~3 times, the culture medium of root is removed, is then planted in compost seed plate i.e.
It can.
Experiment
The cultural method of 1-3 of the embodiment of the present invention and comparative example 1-4 is respectively adopted, is counted after culture and calculates sterile bud
Tissue inductivity and rooting culture seedling growth survival, it is specific to induce result as shown in table 1 below.
1 cultivation results of table
| It organizes inductivity (%) | Growth survival (%) | |
| Embodiment 1 | 52 | 96 |
| Embodiment 2 | 58 | 95 |
| Embodiment 3 | 62 | 98 |
| Comparative example 1 | 38 | 86 |
| Comparative example 2 | 22 | 88 |
| Comparative example 3 | 36 | 76 |
| Comparative example 4 | 28 | 78 |
It can be seen that from upper table 1, dragon fruit tissue cultures carried out using 1-3 method of the embodiment of the present invention, tissue can be turned out
Seedling, tissue inductivity are up to 62%, and organize the growth survival of seedling high, realize the purpose rapidly and efficiently bred.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.
Claims (6)
1. a kind of method of dragon fruit tissue cultivating and seedling, which is characterized in that include the following steps：
S1：Acquire tender stem-tip tissue from the excellent maternal plant of robust growth, by its in gnotobasis incision at length be 4~
The segment of 5cm, is rinsed well with tap water, is carefully peelled off after piercing the spinule on seat and fur removal marrow, then rushed with sterile water
Wash clean, then it is put in 20~30s of immersion in alcoholic solution, 10~12min then is sterilized with 0.1% mercuric chloride solution, uses sterile water
It is explant after rinsing 5 times, then after blotting surface moisture with aseptic filter paper；
S2：Explant described in step S1 is inoculated into 35~42d of induced medium culture and obtains sterile bud, the induced medium
For MS culture medium+0.08~0.12mg/L NAA+2~3mg/L 6-BA+0.5~0.7mg/L IAA+35~40g/L sucrose+5
~7g/L agar, it is 5.7~5.9 that above-mentioned substance, which is fitted into culture bottle, and adjusts pH value；
S3：Sterile bud described in step S2 is put into seedling culture medium and carries out 25~30d of cultivation and obtains young shoot, the seedling culture
Base is+0.08~0.10mg/L of MS culture medium NAA+2.0~2.5mg/L 6-BA+30~32g/L sucrose+4.5~5.2g/L fine jade
Rouge+0.03%~0.04% active carbon；
S4：Young shoot described in step S3 is continued to cultivate to length and is obtained greater than 20~30d of cultivation in root media after 2cm, is moved to
To seedling, the root media be 1/2MS culture medium+0.15~0.20mg/L NAA+0.1~0.2mg/L 6-BA+30~
32g/L sucrose+4.5~5.2g/L agar；
S5：Seedling described in step S4 is subjected to rooting culture.
2. the method for dragon fruit tissue cultivating and seedling according to claim 1, which is characterized in that in step sl, the wine
The volumetric concentration of smart solution is 70~75%.
3. the method for dragon fruit tissue cultivating and seedling according to claim 1, which is characterized in that in step S2~S4, institute
Cultivating process is stated, to be carried out in the case where temperature is 24~26 DEG C, illumination is 2000~2600LX, time of the illumination is 13~
14h/ days.
4. the method for dragon fruit tissue cultivating and seedling according to claim 1, which is characterized in that in step s3, it is described at
The pH value of seedling culture medium is 5.7~5.9.
5. the method for dragon fruit tissue cultivating and seedling according to claim 1, which is characterized in that in step s 4, the life
The pH value of root culture medium is 5.8~6.0.
6. the method for dragon fruit tissue cultivating and seedling according to claim 1, which is characterized in that in step s 5, described to tame and docile
Change is, with carbendazim solution soaking and washing seedling 2~3 times, to remove the culture medium of root, so by seedling after bottle outlet in culture bottle
It is planted in compost seed plate afterwards.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108770695A (en) * | 2018-09-10 | 2018-11-09 | 西南林业大学 | A kind of method of the in vitro tissue culture sprouting and rooting of red heart dragon fruit |
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| CN108770695A (en) * | 2018-09-10 | 2018-11-09 | 西南林业大学 | A kind of method of the in vitro tissue culture sprouting and rooting of red heart dragon fruit |
| CN108770695B (en) * | 2018-09-10 | 2022-01-21 | 西南林业大学 | Method for in-vitro tissue culture and rapid seedling establishment of red-heart pitaya |
| CN113170734A (en) * | 2021-06-02 | 2021-07-27 | 中国热带农业科学院橡胶研究所 | Tissue culture, transplantation and domestication method for bird's nest fruit |
| CN113170734B (en) * | 2021-06-02 | 2022-11-11 | 中国热带农业科学院橡胶研究所 | Tissue culture, transplantation and domestication method for bird's nest fruit |
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