CN1965643B - Tissue culture method of Hosta plantiginea Ascherson - Google Patents
Tissue culture method of Hosta plantiginea Ascherson Download PDFInfo
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- CN1965643B CN1965643B CN2006100474215A CN200610047421A CN1965643B CN 1965643 B CN1965643 B CN 1965643B CN 2006100474215 A CN2006100474215 A CN 2006100474215A CN 200610047421 A CN200610047421 A CN 200610047421A CN 1965643 B CN1965643 B CN 1965643B
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Abstract
The invention relates to a method for cultivating jade needle organism, wherein it comprises that: using fresh bud, via initial cultivation to form germ-free seed, without cure organism to induce the bud to increment, to root and cultivate. The invention has simple process and high yield.
Description
Technical field
The present invention relates to the fragrant plantain lily flower method for tissue culture, belong to field of plant tissue culture technique.
Background technology
Fragrant plantain lily flower originates in east, is widely used all over the world as ornamental plants in garden and medicinal plant.Originating in the jade hairpin (Hosta plantiginea Ascherson) of China, have another name called beautiful spring rod, white crane celestial being, white calyx, is unique type of spending in vain and having strong fragrance of opening in the hosta.Complete stool has little poison, flower to have the effect of clearing heat and moistening lung.But the white jade hairpin adopts the breeding of plant division method at natural world, and growth and reproduction speed are very slow, have restricted its extensive use.If can then be expected to produce at short notice the white jade hairpin test-tube plantlet of a large amount of proterties unanimities by the method breeding of tissue culture.
At present, there is the comparatively deep research of system the aspects such as classification, cultivation and breeding in areas such as America and Europe and Japan to hosta, and China is still inadequate to the utilization exploitation dynamics of Hosta germ plasm resource.Have only on a small quantity about Hosta germ plasm resource and Landscape Application in all kinds of at home documents and materials, view and admire report, then do not appear in the newspapers about the tissue culture technique of spending jade hairpin in vain with the aspects such as tissue culture of jade hairpin kind.
Summary of the invention
At the problems referred to above, the object of the present invention is to provide a kind of jade hairpin method for tissue culture, simple to operate, the reproduction rate height can be produced white jade hairpin test-tube plantlet in a large number.
Purpose of the present invention can be achieved through the following technical solutions,
A kind of tissue culture method of Hosta plantiginea Ascherson is provided, and concrete steps are as follows:
1) selection of explant and sterilization
Select New Development then for use, the axillalry bud that length is about 1 centimetre is with the flowing water flushing 30 minutes after 5～8 minutes of washing powder water rinse, on superclean bench, handled for 10 seconds with 70% alcohol, soak with 0.1% mercuric chloride again and vibrated 6～8 minutes, sterile water wash 4～6 times, standby with the filter paper suck dry moisture.
2) initial culture
Jade hairpin young shoot after the sterilization is seeded in MS+BA
0.2～0.8mg/L+ NAA
0.01～0.1mg/LOn the medium, 20～25 ℃ of cultivation temperature, intensity of illumination 2000Lux, illumination every day 14 hours.
3) shoot proliferation
Proliferated culture medium is: MS+BA
1～3mg/L+ NAA
0.05～0.5mg/L. the jade hairpin aseptic seedling is peelled off the outside blade inoculation that has launched in proliferated culture medium, promptly had axillary bud sprouting to form the bud clump in 25～35 days.
4) culture of rootage
Big and healthy and strong bud is forwarded to root media (MS+NAA
0～0.05mg/L) in, carry out culture of rootage, get final product acclimatization and transplants after treating to have about 20 days 1～3 root to send.
6-BA among the present invention, NAA is plant growth regulator.
The invention has the beneficial effects as follows:
The invention provides the method for tissue culture of a kind of fragrant plantain lily flower, adopting the jade hairpin young shoot is that explant carries out tissue culture, simple to operate, the reproduction coefficient height, owing to do not utilize callus directly to induce axillary bud sprouting to reduce the probability that in expanding numerous process, produces variation, can be used for batch production production.
Embodiment
Embodiment 1:
1, cuts the young shoot of about 1 centimetre of the length of New Development then spring, with the flowing water flushing 30 minutes after 5 minutes of washing powder water rinse, on superclean bench, handled for 10 seconds with 70% alcohol, soak with 0.1% mercuric chloride again and vibrated 6 minutes, sterile water wash 6 times, use the filter paper suck dry moisture, insert inducing culture: MS+BA
0.2mg/L+ NAA
0.01mg/L, additional agar powder 0.6%, sucrose 3%.20 ℃ of cultivation temperature, intensity of illumination 2000Lux, illumination every day 14 hours.After 35 days, basically form aseptic seedling.Pollution rate 28%, planting percent 100%.
2, test-tube plantlet is peelled off the outside blade inoculation that has launched in MS+BA
2mg/L+ NAA
0.1mg/LMedium on, begin to sprout new axillalry bud from exposed leaf scar about 15 days.Most axillalry buds are long more than 1 centimetre after 35 days, can cut apart switching once more.Average bud breaks up several 3.34.
3, switching the time is selected big and healthy and strong bud grafting and is gone into the MS medium and carry out culture of rootage, has root to sprout after 20 days, when treating long 0.5 centimetre of root, transplants to matrix after the uncork hardening and tames.Rooting rate 100%, domestication survival rate 93.3%.
Embodiment 2:
Repeat embodiment 1 process, additional hormone BA0.5mg/L, NAA0.05mg/L in the inducing culture.Planting percent 100%.
Proliferated culture medium is MS+BA
3mg/L+ NAA
0.3mg/L, bud breaks up several 3.67.
Claims (1)
1. a tissue culture method of Hosta plantiginea Ascherson is characterized in that this method comprises the steps:
(1) sterilization: select New Development then for use, the axillalry bud that length is 1 centimetre, adopt the flowing water flushing 30 minutes after 5～8 minutes of washing powder water rinse, on superclean bench, handled for 10 seconds with 70% alcohol, soak with 0.1% mercuric chloride again and vibrated 6～8 minutes, sterile water wash 4～6 times, standby with the filter paper suck dry moisture;
(2) initial culture: the explant after will sterilizing is seeded in MS+BA
0.2～0.8mg/L+ NAA
0.01～0.1mg/LOn the medium, cultivation temperature is 20～25 ℃, intensity of illumination 2000Lux, illumination every day 14 hours;
(3) shoot proliferation: at MS medium supplemented plant growth regulating substance BA1～3mg/L, NAA 0.05～0.5mg/L, the jade hairpin aseptic seedling is peelled off the outside blade inoculation that has launched in above-mentioned proliferated culture medium, induce axillary bud sprouting to form the bud clump;
(4) culture of rootage: big and healthy and strong bud is forwarded to root media: MS+NAA
0～0.05mg/L, carry out culture of rootage, there is 1～3 root to send the back acclimatization and transplants.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100474215A CN1965643B (en) | 2006-08-10 | 2006-08-10 | Tissue culture method of Hosta plantiginea Ascherson |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100474215A CN1965643B (en) | 2006-08-10 | 2006-08-10 | Tissue culture method of Hosta plantiginea Ascherson |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1965643A CN1965643A (en) | 2007-05-23 |
| CN1965643B true CN1965643B (en) | 2011-04-06 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2006100474215A Expired - Fee Related CN1965643B (en) | 2006-08-10 | 2006-08-10 | Tissue culture method of Hosta plantiginea Ascherson |
Country Status (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283130A (en) * | 2011-07-22 | 2011-12-21 | 福建农林大学 | Method for culturing and fast propagating tissue of chimaera hosta plantaginea plant |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103875535B (en) * | 2014-04-03 | 2015-09-02 | 中国科学院植物研究所 | A kind of method of floral leaf jade hairpin fast breeding |
| CN104304035B (en) * | 2014-11-14 | 2016-06-15 | 黑龙江省科学院自然与生态研究所 | The tissue cultivation rapid breeding method of golden-rimmed jade hairpin |
| CN106135004A (en) * | 2016-08-25 | 2016-11-23 | 广西壮族自治区药用植物园 | A kind of quick breeding method for tissue culture of Flos hostae ventricosae |
| CN106900307A (en) * | 2017-01-05 | 2017-06-30 | 天津泰达绿化集团有限公司 | A kind of division propagation method of jade hairpin |
| CN110972945A (en) * | 2019-12-19 | 2020-04-10 | 美尚生态景观股份有限公司 | Culture medium and culture method for tissue culture of plantain plantaginea |
| CN111802379B (en) * | 2020-06-23 | 2021-11-09 | 中国农业科学院作物科学研究所 | Method for storing test-tube plantlets of Hosta plantaginea |
-
2006
- 2006-08-10 CN CN2006100474215A patent/CN1965643B/en not_active Expired - Fee Related
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283130A (en) * | 2011-07-22 | 2011-12-21 | 福建农林大学 | Method for culturing and fast propagating tissue of chimaera hosta plantaginea plant |
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| CN1965643A (en) | 2007-05-23 |
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Granted publication date: 20110406 Termination date: 20110810 |