CN105028214A - Efficient expanding propagation method for dahlia toxin-free seedlings - Google Patents

Efficient expanding propagation method for dahlia toxin-free seedlings Download PDF

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CN105028214A
CN105028214A CN201510569349.1A CN201510569349A CN105028214A CN 105028214 A CN105028214 A CN 105028214A CN 201510569349 A CN201510569349 A CN 201510569349A CN 105028214 A CN105028214 A CN 105028214A
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dahlia
seedling
culture
nutrient solution
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CN105028214B (en
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不公告发明人
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Jinan Yiran Seedling Planting Co ltd
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Abstract

The invention discloses an efficient expanding propagation method for dahlia toxin-free seedlings. The method includes the following steps of 1, pretreatment of dahlia toxin-free seedlings, 2, induction of adventitious buds, 3, cutting of adventitious buds for mulitiplication, 4, hardening-seedling, 5, water planting and rooting, 6, water planting of strong seedlings and 7 transplanting. The efficient expanding propagation method solves the problems that dahlia toxin-free seedlings are low in efficiency in the expanding propagation process and prone to secondary pollution. A great number of excellent dahlia toxin-free seedlings can be cultured in an expanding propagation mode in a short period of time, the problem of variety degeneration brought by dahlia division propagation and seed propagation can be avoided, and the method is easy to operate and has higher practical value in production, and industrialized popularization is achieved easily.

Description

A kind of efficient expanding propagation method of dahlia virus-elimination seedlings
Technical field
The present invention relates to a kind of efficient expanding propagation method of dahlia virus-elimination seedlings, be specifically related to a kind ofly to expand the expanding propagation method of numerous a large amount of excellent dahlia virus-elimination seedlings in the short time, belong to technical field of agriculture science.
Background technology
Dahlia ( dahliapinnatacav) be the perennial flowering bulb of composite family dahlia, full genus about 27 kinds, have another name called Dali chrysanthemum, dahlia, passionflower, foreign Chinese herbaceous peony, large beautiful chrysanthemum, pachyrhizus, sweet potato flower etc., originate in the state such as Mexico, Colombia and Guatemala, after pass to Europe, Japan and China.Dahlia cane is sturdy, and blade is loose, and flower shape is similar to tree peony, spends large and beautiful in colour, has the features such as colored appearance is graceful, flower pattern is various, the florescence is long, various in style, is described as the favorite in flowers.Dahlia is natural interspecific cross, and the history of artificially breeding is not long, and countries in the world are generally cultivated, and at present northeastward, northwest, the cultivation of the ground such as North China be comparatively extensive, wherein many at Guangdong autumn, winter two season cultivation.Select through artificial hybridization, become allooctaploid, new varieties emerge in an endless stream, and pattern is delicate and charming, and the florescence is long, strong adaptability, are easy to cultivation, therefore suddenly become world's famous flower.Current dahlia is the city flower of Mexican national flower, Seattle, and the province in Jilin Province of China spends, the city flower in the city such as Zhangjiakou, Chifeng, packet header (little beautiful flower), and dahlia symbol is generous, gorgeous, and good luck is world-renowned ornamental flower.
Dahlia adopts point root and cottage propagation usually, but point root and cottage propagation reproduction coefficient low, but every strain is often only and can be separated 5-6 new strain, the requirement that industrialization is produced cannot be met, and use division propagation always, easily make virus by generation accumulation, thus cause dahlia deterioration of variety serious.
Utilize the technology such as tissue cultures, rapid propagation in vitro can solve the problem of the accumulation of dahlia virus disease and Rapid Popularization.More domestic researchers adopt dahlia explant; some Study on tissue culture are carried out; obtain some progress; but the tissue culture technique studies in China of dahlia is started late; research accumulation is few; problem that quick-breeding method after dahlia detoxification exists that inductivity is lower, induction seedling is of poor quality and how weak seedling, seedling proliferation efficiency is low, transplantation of seedlings survival rate is low etc., seriously constrains that dahlia kind is seedling industrialized, large-scale production.Therefore, establish and improve the dahlia virus-elimination seedlings multiplication technique of simple economy, to the batch production of promotion dahlia virus-elimination seedlings, large-scale production, to the popularization of dahlia improved seeds, there are wide market prospects.
Summary of the invention
For the problem that solves that the inductivity that current dahlia detoxic seedling expanding propagation method exists is lower, induction seedling is of poor quality and how weak seedling, seedling proliferation efficiency is low, transplantation of seedlings survival rate is low etc., the invention provides a kind of efficient expanding propagation method of dahlia virus-elimination seedlings, by having established and improve the proportioning of cultivation program, condition of culture and cultivation component, improve quality and proliferate efficiency, the transplanting survival rate of induction seedling, thus establish high-quality and high quantity rapid propagation system.
The object of the invention is to solve by the following technical programs:
An efficient expanding propagation method for dahlia virus-elimination seedlings, is characterized in that: the method step is as follows:
1) dahlia detoxic seedling pre-treatment: cleaned up by dahlia detoxic seedling and surface sterilization, aseptically cut petiole and holder, moves into and is equipped with in the adventitious bud induction culture base wide-mouth bottle prepared;
2) induction of indefinite bud: culturing room's light inoculation being had the adventitious bud induction culture base wide-mouth bottle of detoxic seedling explant to be placed in disinfect is cultivated, and induces indefinite bud;
3) cut indefinite bud shoot proliferation: aseptically cut by indefinite bud, move into be equipped with in the wide-mouth bottle of proliferated culture medium and cultivate, obtain a large amount of dahlia seedling;
4) hardening: the method for progressively employing enhancing cultivation illumination, reduction cultivation temperature carries out hardening;
5) water planting rooting treatment: taken out from wide-mouth bottle by the detoxification test tube plantlet after hardening, retains original root system, then is planted by plantlet in vitro in the cultivation plate in water culture disc, adds nutrient solution of taking root in water culture disc;
6) water planting strong sprout: culture of rootage, after 7 days, outwells nutrient solution of taking root, and adds nutrient solution in strong sprout;
7) transplant: detoxic seedling is transplanted in sterile matrix, grows under being placed in outdoor natural light.
The detailed step of the method is as follows:
1) dahlia detoxic seedling pre-treatment:
Dahlia detoxic seedling is first rinsed repeatedly with running water, with hairbrush dip liquid detergent wash one time after then clean with ultrapure water, with 70% alcohol-pickled 15s, use aseptic water washing twice again, in 0.1% mercuric chloride, addend drips polysorbas20 solution and soaks 5min, with aseptic water washing 4-5 time, filter paper blots, and the petiole of dahlia detoxic seedling and holder is moved into and is equipped with in the wide-mouth bottle of adventitious bud induction culture base;
2) induction of indefinite bud:
For anti-dahlia detoxic seedling is contaminated by virus in process of production again, culturing room is first through sterilization, and 40% formaldehyde 10ml/m selected by disinfectant 3, potassium permanganate 5g/m 3stifling 2 hours are carried out to whole culturing room, after stifling, adopt 75% ethanol culturing rack to carry out surface sterilization again, then cultivated by culturing room's light that inoculation has the adventitious bud induction culture base wide-mouth bottle of dahlia detoxic seedling explant to be placed in disinfect, it is 26 ± 1 DEG C that culturing room's temperature controls, take fluorescent lamp as light source, intensity of illumination controls as 1500-2000Lx, and the photoperiod controls as within the dark 10h of light 14h/, 25-30 days, to induce indefinite bud;
3) indefinite bud shoot proliferation is cut:
Aseptically by indefinite bud complete cutting from explant of induction 2-3cm, be transferred to and be equipped with in the wide-mouth bottle of proliferated culture medium, it is 24 ± 1 DEG C that culturing room's temperature controls, intensity of illumination controls as 1500-2000Lx, photoperiod control, for cultivating under the dark 12h of light 12h/, to cultivate after 15-25 days and can obtain a large amount of dahlia seedling;
4) hardening:
The dahlia detoxification test tube plantlet of screening 5-8cm, transferring to intensity of illumination is under the condition of 2500-3500lx, after 3-5 days, cultivation temperature is reduced to 20-22 DEG C, after 3-5 days, opens wide-mouth bottle sealing, continues hardening 3-5 days;
5) water planting rooting treatment:
For reducing the injury of transferring and bringing detoxification test tube plantlet root system, a small amount of water is added in switching forward direction wide-mouth bottle, and stir medium gently with tweezers, in order to avoid damage root when young plant is poured out, test-tube plantlet pours out the medium on afterwash root, be transplanted in the cultivation plate in the water culture disc that culture of rootage liquid is housed, planting density 1000-1200 seedling/m 2spacing in the rows 2-3cm, line-spacing 2-3cm, length × wide=44 × 32cm at the bottom of water planting vessel basin, basin is high=plastic basin of 15cm as cultivation tray, every basin puts 2L nutrient solution, and has field planting hole spacing to be that the cultivation plate of 2cm-3cm swims on nutrient solution, the plastic film of layer of transparent will be costed above detoxic seedling, make that it is in temperature 22-25 DEG C, humidity>=90%, intensity of illumination be the clean greenhouse environment growth of 2000-3000lx;
6) water planting strong sprout:
Culture of rootage is after 7 days, and detoxic seedling root system is grown completely substantially, now, removes plastic film, outwells nutrient solution of taking root, and waters execute nutrient solution in strong sprout to seedling;
7) transplant:
When dahlia detoxic seedling grows to 12-15cm, be transplanted in sterile matrix, Transplanting Density is line-spacing 15cm, spacing in the rows 10cm, and covered rearing with plastic film moisturizing progressively removed film after 10-15 days, grew under being placed in outdoor natural light.
Step 1) and 2) in adventitious bud induction culture based formulas be: MS+2.5mg/L6-BA+0.08NAA+3% sucrose+6.5g/L plant gel, pH is 5.8.
Proliferation culture medium formula in step 3) is: MS+3.0mg/L6-BA+0.5mg/LNAA+0.5mg/LGA+2.5mg/L Choline Chloride+4% sucrose+6.0g/L agar, pH is 5.8.
Step 5) and 6) in culture of rootage formula of liquid be: 500mg/LKNO 3, 500mg/LNH 4nO 3, 92.5mg/LMgSO 47H 2o, 173mg/LCa (NO 3) 24H 2o, 150mg/LKH 2pO 4, 32mg/LKCl, 17mg/LMnSO 47H 2o, 19mg/LNa 2-EDTA, 14mg/LFeSO 47H 2o, 2.2mg/LMnSO 44H 2o, 0.8mg/LZnSO 47H 2o, 0.8mg/LH 3bO 3, 0.4mg/LKI, 50mg/L inositol, 1mg/L glycine, 0.05mg/LVB 1, 0.05mg/LVB 6, be mixed and made into nutrient solution of taking root.
Step 5) and 6) in the optional formula of another kind of culture of rootage liquid be: 950mg/LKNO 3, 825mg/LNH 4nO 3, 85mg/LKH 2pO 4, 220mg/LCaCl 22H 2o, 185mg/LMgSO 47H 2o, 19mg/LNa 2-EDTA, 14mg/LFeSO 47H 2o, 11mg/LMnSO 44H 2o, 0.4mg/LKI, 3.1mg/LH 3bO 3, 4.3mg/LZnSO 47H 2o, 0.125mg/LNa 2moO 4, 0.0125mg/LCuSO 4, 0.0125mg/LCoCl 26H 2o, 50mg/L inositol, 1mg/L glycine, 0.05mg/LVB 1, 0.05mg/LVB 6, be mixed and made into nutrient solution of taking root.
Nutrient solution prescription in strong sprout in step 6) is: 850mg/LKNO 3, 750mg/LNH 4nO 3, 165mg/LMgSO 47H 2o, 75mg/LKH 2pO 4, 200mg/LCaCl 22H 2o, 20mg/LNa 2-EDTA, 15mg/LFeSO 47H 2o, 10.5mg/LMnSO 44H 2o, 4.25mg/LZnSO 47H 2o, 3.0mg/LH 3bO 3, 0.4mg/LKI, 0.12mg/LNa 2m oo 42H 20,0.012mg/LCoC1 26H 2o, 0.012mg/LCuSO 45H 2o, 0.4mg/LKI, 30mg/L inositol, 0.5mg/L glycine, 0.05mg/LVB 1, 0.05mg/LVB 6, be mixed and made into nutrient solution in strong sprout.
Sterile matrix components in step 7) is the peat composed of rotten mosses, perlite, the vermiculite of equal-volume ratio.
Beneficial effect of the present invention:
(1) sterilization method of the present invention is simple, effectively, explant Disinfection Effect is good, and the pollution rate of explant is reduced to less than 5%;
(2) the invention solves the shortcoming that prior art inductivity is lower, proliferate efficiency is low, adventitious bud induction frequency improves about 30% than average level; Adventitious bud proliferation efficiency improves about 30% than average level; The numerous efficiency of final expansion improves about 70%;
(3) the invention solves the shortcoming that poor, the weak seedling of prior art seedling quality is many, the seedling quality of acquisition is high, and weak seedling or death rate are reduced to only has about 1%;
(4) transplantation of seedlings survival rate of the present invention improves greatly than prior art, and transplanting survival rate reaches about 99%.
Accompanying drawing explanation
Accompanying drawing 1 is the result figure of dahlia adventitious bud inducing;
Accompanying drawing 2 is the result figure of dahlia adventitious bud proliferation;
Accompanying drawing 3 is the result figure obtaining high-quality dahlia seedling under this technology path.
Embodiment
Further describe concrete technical scheme of the present invention below in conjunction with embodiment and accompanying drawing, so that those skilled in the art understands the present invention further, and do not form the restriction to its right.
Embodiment
The efficient expanding propagation method of the dahlia virus-elimination seedlings of the present embodiment, the enforcement time is 2012-2013 years, the pre-treatment of dahlia detoxic seedling, plant regeneration and hardening are carried out in development flowers garden, Lianyungang, plantlet in vitro is transplanted and is carried out in flowers proving ground, Dong Xin farm, Lianyungang, and detailed step is as follows:
1) dahlia detoxic seedling pre-treatment:
Dahlia detoxic seedling is first rinsed repeatedly with running water, with hairbrush dip liquid detergent wash one time after then clean with ultrapure water, with 70% alcohol-pickled 15s, use aseptic water washing twice again, in 0.1% mercuric chloride, addend drips polysorbas20 solution and soaks 5min, with aseptic water washing 5 times, filter paper blots, the petiole of dahlia detoxic seedling and holder immigration are equipped with adventitious bud induction culture base, and (culture medium prescription is: MS+2.5mg/L6-BA+0.08NAA+3% sucrose+6.5g/L plant gel, pH is 5.8) wide-mouth bottle in, for contrasting the effect of adventitious bud induction culture base of the present invention, have selected another 2 kinds of adventitious bud induction culture bases simultaneously, culture medium prescription is respectively: MS+BA1.0mg/L+NAA0.8mg/L+3% sucrose+5g/L agar, pH is that 5.8(documents comes from " tissue cultures of dahlia " such as Tian Muzhen), MS+6-BA0.5mg/L+NAA0.1mg/L+3% sucrose+6g/L agar, pH is that 5.8(documents comes from " the tissue cultures Primary Study of dahlia " such as Qiu Jing),
2) induction of indefinite bud:
For anti-dahlia detoxic seedling is contaminated by virus in process of production again, culturing room is first through sterilization, and 40% formaldehyde 10ml/m selected by disinfectant 3, potassium permanganate 5g/m 3, stifling 2 hours are carried out to whole culturing room, after stifling, 75% ethanol culturing rack is adopted to carry out surface sterilization again, then culturing room's light that inoculation has the adventitious bud induction culture base wide-mouth bottle of dahlia detoxic seedling explant to be placed in disinfect is cultivated, it is 26 DEG C that culturing room's temperature controls, take fluorescent lamp as light source, intensity of illumination controls as 1800Lx, photoperiod controls as the dark 10h of light 14h/, indefinite bud is induced after 25 days, result is as Fig. 1, statistics adventitious bud induction frequency, adventitious bud induction frequency of the present invention reaches 69.8%, be significantly higher than the adventitious bud induction frequency of other 3 average about 46%, adventitious bud induction frequency statistics is as table 1,
3) indefinite bud shoot proliferation is cut:
Aseptically by indefinite bud complete cutting from explant of induction 2-3cm, be transferred to and proliferated culture medium (MS+3.0mg/L6-BA+0.5mg/LNAA+0.5mg/LGA+2.5mg/L Choline Chloride+4% sucrose+6.0g/L agar is housed, pH is 5.8) wide-mouth bottle in, for contrasting the effect of proliferated culture medium of the present invention, have selected another 2 kinds of proliferated culture mediums simultaneously, culture medium prescription is respectively: MS+1.5mg/LBA+0.2mg/LNAA+3% sucrose+5g/L agar, pH is that 5.8(documents comes from " tissue cultures of dahlia " such as Tian Muzhen), MS+6-BA0.5mg/L+NAA0.1mg/L+3% sucrose+6g/L agar, pH is that 5.8(documents comes from " the tissue cultures Primary Study of dahlia " such as Qiu Jing), then culturing room's temperature is controlled to be 24 ± 1 DEG C, intensity of illumination controls as 1800Lx, photoperiod controls as cultivating under the dark 12h of light 12h/, cultivate and a large amount of dahlia seedling within about 20 days, can be obtained, as Fig. 2, repeatedly the Multiple Buds of formation is cut into simple bud, be inoculated on proliferated culture medium and carry out Multiplying culture, dahlia rapid, high volume ground propagation, medium of the present invention reaches 6.8 to dahlia increment multiple, be significantly higher than other proliferation times of about 4.7, each medium is as shown in table 2 to dahlia increment multiple,
4) hardening:
The dahlia detoxification test tube plantlet of screening 5-8cm, transferring to intensity of illumination is under the condition of about 3000lx, after 3-5 days, cultivation temperature is reduced to 20-22 DEG C, after 3-5 days, opens wide-mouth bottle sealing, continues hardening 3-5 days;
5) water planting rooting treatment:
For reducing the injury of transferring and bringing detoxification test tube plantlet root system, a small amount of water is added in switching forward direction wide-mouth bottle, and stir medium gently with tweezers, in order to avoid damage root when young plant is poured out, test-tube plantlet pours out the medium on afterwash root, is transplanted to culture of rootage liquid to be housed (culture fluid formula is: 500mg/LKNO 3, 500mg/LNH 4nO 3, 92.5mg/LMgSO 47H 2o, 173mg/LCa (NO 3) 24H 2o, 150mg/LKH 2pO 4, 32mg/LKCl, 17mg/LMnSO 47H 2o, 19mg/LNa 2-EDTA, 14mg/LFeSO 47H 2o, 2.2mg/LMnSO 44H 2o, 0.8mg/LZnSO 47H 2o, 0.8mg/LH 3bO 3, 0.4mg/LKI, 50mg/L inositol, 1mg/L glycine, 0.05mg/LVB 1, 0.05mg/LVB 6, be mixed and made into nutrient solution of taking root) water culture disc in cultivation plate on, planting density 1000-1200 seedling/m 2spacing in the rows 2-3cm, line-spacing 2-3cm, length × wide=44 × 32cm at the bottom of water planting vessel basin, basin is high=plastic basin of 15cm as cultivation tray, every basin puts 2L nutrient solution, and has field planting hole spacing to be that the cultivation plate of 2cm-3cm swims on nutrient solution, the plastic film of layer of transparent will be costed above detoxic seedling, make that it is in temperature 22-25 DEG C, humidity>=90%, intensity of illumination be the clean greenhouse environment growth of 2000-3000lx;
6) water planting strong sprout:
Culture of rootage is after 7 days, and detoxic seedling root system is grown completely substantially, now, removes plastic film, outwells nutrient solution of taking root, and waters execute nutrient solution in strong sprout to seedling, the weak seedling rate of dead seedling of seedling after statistics propagation, and the weak seedling rate of the dead seedling of result only has 2.7%;
7) transplant:
When dahlia detoxic seedling grows to 12-15cm, be transplanted in sterile matrix, Transplanting Density is line-spacing 15cm, spacing in the rows 10cm, covered rearing with plastic film moisturizing, progressively remove film after 10-15 days, grow under being placed in outdoor natural light, transplanting survival rate statistics reaches 99%, and obtain the dahlia seedling of high-quality, as Fig. 3.
By the visible Be very effective of the present invention of the present embodiment: (1) is in step 2) in, 69.8% is reached to the adventitious bud induction frequency of dahlia, is significantly higher than the adventitious bud induction frequency of conventional 46%; Statistic procedure 3) middle adventitious bud proliferation efficiency, proliferation times reaches 6.8, is significantly higher than the proliferation times of conventional 4.7; The numerous efficiency of final expansion improves about 1.2 times; (2) seedling quality obtained is high, without damage by disease and insect, weak seedling or death rate only account for 2.7%; (3) transplantation of seedlings survival rate improves greatly than prior art, and transplanting survival rate reaches about 99%.
Those skilled in the art can make replacement or modification according to content disclosed by the invention and the art technology grasped to content of the present invention; but these replacements or modification should not be considered as disengaging the present invention design, and these replacements or modification are all in the interest field of application claims protection.

Claims (8)

1. an efficient expanding propagation method for dahlia virus-elimination seedlings, is characterized in that: the method step is as follows:
1) dahlia detoxic seedling pre-treatment: cleaned up by dahlia detoxic seedling and surface sterilization, aseptically cut petiole and holder, moves into and is equipped with in the adventitious bud induction culture base wide-mouth bottle prepared;
2) induction of indefinite bud: culturing room's light inoculation being had the adventitious bud induction culture base wide-mouth bottle of detoxic seedling explant to be placed in disinfect is cultivated, and induces indefinite bud;
3) cut indefinite bud shoot proliferation: aseptically cut by indefinite bud, move into be equipped with in the wide-mouth bottle of proliferated culture medium and cultivate, obtain a large amount of dahlia seedling;
4) hardening: the method for progressively employing enhancing cultivation illumination, reduction cultivation temperature carries out hardening;
5) water planting rooting treatment: taken out from wide-mouth bottle by the detoxification test tube plantlet after hardening, retains original root system, then is planted by plantlet in vitro in the cultivation plate in water culture disc, adds nutrient solution of taking root in water culture disc;
6) water planting strong sprout: culture of rootage, after 7 days, outwells nutrient solution of taking root, and adds nutrient solution in strong sprout;
7) transplant: detoxic seedling is transplanted in sterile matrix, grows under being placed in outdoor natural light.
2. the efficient expanding propagation method of dahlia virus-elimination seedlings according to claim 1, is characterized in that: the detailed step of the method is as follows:
1) dahlia detoxic seedling pre-treatment:
Dahlia detoxic seedling is first rinsed repeatedly with running water, with hairbrush dip liquid detergent wash one time after then clean with ultrapure water, with 70% alcohol-pickled 15s, use aseptic water washing twice again, in 0.1% mercuric chloride, addend drips polysorbas20 solution and soaks 5min, with aseptic water washing 4-5 time, filter paper blots, and the petiole of dahlia detoxic seedling and holder is moved into and is equipped with in the wide-mouth bottle of adventitious bud induction culture base;
2) induction of indefinite bud:
For anti-dahlia detoxic seedling is contaminated by virus in process of production again, culturing room is first through sterilization, and 40% formaldehyde 10ml/m selected by disinfectant 3, potassium permanganate 5g/m 3stifling 2 hours are carried out to whole culturing room, after stifling, adopt 75% ethanol culturing rack to carry out surface sterilization again, then cultivated by culturing room's light that inoculation has the adventitious bud induction culture base wide-mouth bottle of dahlia detoxic seedling explant to be placed in disinfect, it is 26 ± 1 DEG C that culturing room's temperature controls, take fluorescent lamp as light source, intensity of illumination controls as 1500-2000Lx, and the photoperiod controls as within the dark 10h of light 14h/, 25-30 days, to induce indefinite bud;
3) indefinite bud shoot proliferation is cut:
Aseptically by indefinite bud complete cutting from explant of induction 2-3cm, be transferred to and be equipped with in the wide-mouth bottle of proliferated culture medium, it is 24 ± 1 DEG C that culturing room's temperature controls, intensity of illumination controls as 1500-2000Lx, photoperiod control, for cultivating under the dark 12h of light 12h/, to cultivate after 15-25 days and can obtain a large amount of dahlia seedling;
4) hardening:
The dahlia detoxification test tube plantlet of screening 5-8cm, transferring to intensity of illumination is under the condition of 2500-3500lx, after 3-5 days, cultivation temperature is reduced to 20-22 DEG C, after 3-5 days, opens wide-mouth bottle sealing, continues hardening 3-5 days;
5) water planting rooting treatment:
For reducing the injury of transferring and bringing detoxification test tube plantlet root system, a small amount of water is added in switching forward direction wide-mouth bottle, and stir medium gently with tweezers, in order to avoid damage root when young plant is poured out, test-tube plantlet pours out the medium on afterwash root, be transplanted in the cultivation plate in the water culture disc that culture of rootage liquid is housed, planting density 1000-1200 seedling/m 2spacing in the rows 2-3cm, line-spacing 2-3cm, length × wide=44 × 32cm at the bottom of water planting vessel basin, basin is high=plastic basin of 15cm as cultivation tray, every basin puts 2L nutrient solution, and has field planting hole spacing to be that the cultivation plate of 2cm-3cm swims on nutrient solution, the plastic film of layer of transparent will be costed above detoxic seedling, make that it is in temperature 22-25 DEG C, humidity>=90%, intensity of illumination be the clean greenhouse environment growth of 2000-3000lx;
6) water planting strong sprout:
Culture of rootage is after 7 days, and detoxic seedling root system is grown completely substantially, now, removes plastic film, outwells nutrient solution of taking root, and waters execute nutrient solution in strong sprout to seedling;
7) transplant:
When dahlia detoxic seedling grows to 12-15cm, be transplanted in sterile matrix, Transplanting Density is line-spacing 15cm, spacing in the rows 10cm, and covered rearing with plastic film moisturizing progressively removed film after 10-15 days, grew under being placed in outdoor natural light.
3. the efficient expanding propagation method of dahlia virus-elimination seedlings according to claim 1 and 2, is characterized in that: step 1) and 2) in adventitious bud induction culture based formulas be: MS+2.5mg/L6-BA+0.08NAA+3% sucrose+6.5g/L plant gel, pH is 5.8.
4. the efficient expanding propagation method of dahlia virus-elimination seedlings according to claim 1 and 2, it is characterized in that: the proliferation culture medium formula in step 3) is: MS+3.0mg/L6-BA+0.5mg/LNAA+0.5mg/LGA+2.5mg/L Choline Chloride+4% sucrose+6.0g/L agar, pH is 5.8.
5. the efficient expanding propagation method of dahlia virus-elimination seedlings according to claim 1 and 2, is characterized in that: step 5) and 6) in culture of rootage formula of liquid be: 500mg/LKNO 3, 500mg/LNH 4nO 3, 92.5mg/LMgSO 47H 2o, 173mg/LCa (NO 3) 24H 2o, 150mg/LKH 2pO 4, 32mg/LKCl, 17mg/LMnSO 47H 2o, 19mg/LNa 2-EDTA, 14mg/LFeSO 47H 2o, 2.2mg/LMnSO 44H 2o, 0.8mg/LZnSO 47H 2o, 0.8mg/LH 3bO 3, 0.4mg/LKI, 50mg/L inositol, 1mg/L glycine, 0.05mg/LVB 1, 0.05mg/LVB 6, be mixed and made into nutrient solution of taking root.
6. the efficient expanding propagation method of dahlia virus-elimination seedlings according to claim 1 and 2, is characterized in that: step 5) and 6) in the optional formula of another kind of culture of rootage liquid be: 950mg/LKNO 3, 825mg/LNH 4nO 3, 85mg/LKH 2pO 4, 220mg/LCaCl 22H 2o, 185mg/LMgSO 47H 2o, 19mg/LNa 2-EDTA, 14mg/LFeSO 47H 2o, 11mg/LMnSO 44H 2o, 0.4mg/LKI, 3.1mg/LH 3bO 3, 4.3mg/LZnSO 47H 2o, 0.125mg/LNa 2moO 4, 0.0125mg/LCuSO 4, 0.0125mg/LCoCl 26H 2o, 50mg/L inositol, 1mg/L glycine, 0.05mg/LVB 1, 0.05mg/LVB 6, be mixed and made into nutrient solution of taking root.
7. the efficient expanding propagation method of dahlia virus-elimination seedlings according to claim 1 and 2, is characterized in that: the nutrient solution prescription in strong sprout in step 6) is: 850mg/LKNO 3, 750mg/LNH 4nO 3, 165mg/LMgSO 47H 2o, 75mg/LKH 2pO 4, 200mg/LCaCl 22H 2o, 20mg/LNa 2-EDTA, 15mg/LFeSO 47H 2o, 10.5mg/LMnSO 44H 2o, 4.25mg/LZnSO 47H 2o, 3.0mg/LH 3bO 3, 0.4mg/LKI, 0.12mg/LNa 2m oo 42H 20,0.012mg/LCoC1 26H 2o, 0.012mg/LCuSO 45H 2o, 0.4mg/LKI, 30mg/L inositol, 0.5mg/L glycine, 0.05mg/LVB 1, 0.05mg/LVB 6, be mixed and made into nutrient solution in strong sprout.
8. the efficient expanding propagation method of dahlia virus-elimination seedlings according to claim 1 and 2, is characterized in that: the sterile matrix components in step 7) is the peat composed of rotten mosses, perlite, the vermiculite of equal-volume ratio.
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CN107306632A (en) * 2017-06-29 2017-11-03 成都苗夫现代苗木科技有限公司 A kind of culturing and reproducing of dahlia
CN108901852A (en) * 2018-07-30 2018-11-30 连云港秀景园林绿化工程有限公司 A kind of industrial fast breeding method of broad-leaved epiphyllum virus-elimination seedlings
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CN111642392A (en) * 2020-04-29 2020-09-11 上海润绣农业科技有限公司 Sterilization method for surface of dahlia explant

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