CN106212286A - A kind of laurustinus special culture media - Google Patents
A kind of laurustinus special culture media Download PDFInfo
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- CN106212286A CN106212286A CN201610653568.2A CN201610653568A CN106212286A CN 106212286 A CN106212286 A CN 106212286A CN 201610653568 A CN201610653568 A CN 201610653568A CN 106212286 A CN106212286 A CN 106212286A
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- nitrate
- laurustinus
- culture medium
- lanthanum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to a kind of laurustinus special culture media, including 60 65 parts of MS basis mother solution, glucose 46 parts, lactoalbumin hydrolysate 68 parts, corn embryosperm 48 parts, Tomato juice 48 parts, Sucus Cocois 2 14 parts, indolebutyric acid 25 parts, naphthalene acetic acid 14 parts, ABT root-inducing powder 26 parts, rare-earth compound 5 15 parts, activated carbon 5 10 parts, 30 35 parts of water.The present invention has simple, the feature that quantity of emerging is many, provides technical support for industrial seedling rearing.
Description
Technical field
The present invention relates to plant culture field, especially a kind of laurustinus special culture media.
Background technology
Silk ball has another name called well Flos Hydrangeae Macrophyllae, hydrangea, for Saxifragaceae Seven barks. plant.Laurustinus flower is bought white plentiful, big and
Beauty, its pattern energy is red can be blue, makes us pleasing and soothes the spirit, is common potted plant ornamencal flower and tree.
At present laurustinus culture medium is less, and it is the best to be difficult to operation, seedling effect, it is difficult to meeting the market requirement.
Summary of the invention
Present invention aims in providing a kind of for laurustinus special culture media.For prior art being difficult to operation, going out
The problems such as Seedling poor effect, the invention provides a kind of practicable solution.The present invention has simple, number of emerging
The feature that amount is many, provides technical support for industrial seedling rearing.
The present invention is for reaching its purpose, and the technical scheme of employing is as follows:
A kind of laurustinus special culture media, described laurustinus special culture media includes MS basis mother solution 60-65 part, glucose
4-6 part, lactoalbumin hydrolysate 6-8 part, corn embryosperm 4-8 part, Tomato juice's 4-8 part, Sucus Cocois 2-14 part, indolebutyric acid 2-5 part,
Naphthalene acetic acid 1-4 part, ABT root-inducing powder 2-6 part, rare-earth compound 5-15 part, activated carbon 5-10 part, water 30-35 part.
Further, the rare-earth compound comprised in culture medium is at least in Lanthanum (III) nitrate, neodymium nitrate, samaric nitrate
Kind.
Further, rare-earth compound adds culture medium as follows to: in superclean bench environment, by nitre
One in acid lanthanum, neodymium nitrate, samaric nitrate is dissolved in water and forms solution, through filter-sterilizer filtration sterilization, will be not added with dilute simultaneously
The culture medium of earth elements compound carries out high-temperature sterilization, before culture medium not yet recovers room temperature, with liquid-transfering gun by Lanthanum (III) nitrate or nitre
The solution of acid neodymium or samaric nitrate is dispensed in each culture medium, and rocks uniformly.
Further, laurustinus special culture media include 60 parts of MS basis mother solution, glucose 4 parts, lactoalbumin hydrolysate 6 parts,
Corn embryosperm 4 parts, Tomato juice 4 parts, Sucus Cocois 4 parts, indolebutyric acid 2 parts, naphthalene acetic acid 2 parts, ABT root-inducing powder 3 parts, rare earth element
Compound 7 parts, activated carbon 6 parts, 32 parts of water.Wherein, rare-earth compound is the mixture of Lanthanum (III) nitrate and neodymium nitrate, wherein nitre
Acid lanthanum is 3:4 with the mass ratio of neodymium nitrate.Wherein, rare-earth compound can also be Lanthanum (III) nitrate, neodymium nitrate and samaric nitrate
Mixture, wherein the mass ratio 2:2:3 of Lanthanum (III) nitrate, neodymium nitrate and samaric nitrate.
Laurustinus special culture media in the present invention is primarily as adventitious bud culture medium；In the present invention it is further proposed that
Laurustinus plantlet culture medium, described laurustinus plantlet culture medium be MS basis mother solution 45-50 part, glucose 2-5 part,
Lactoalbumin hydrolysate 4-6 part, corn embryosperm 2-4 part, peach juice 2-4 part, Sucus Cocois 2-10 part, heteroauxing 2-5 part, naphthoxyacetic acid 1-
4 parts, ABT root-inducing powder 2-6 part, rare-earth compound 5-15 part, activated carbon 5-10 part, water 20-30 part.
Further, the rare-earth compound in described laurustinus plantlet culture medium is Lanthanum (III) nitrate, neodymium nitrate, nitre
At least one in acid samarium.
Further, laurustinus plantlet culture medium includes 45 parts of MS basis mother solution, glucose 2 parts, lactoalbumin hydrolysate 4
Part, corn embryosperm 2 parts, peach juice 3 parts, Sucus Cocois 2 parts, heteroauxing 2 parts, naphthoxyacetic acid 2 parts, ABT root-inducing powder 3 parts, rare earth unit
Element compound 6 parts, activated carbon 5 parts, 30 parts of water.
The beneficial effects of the present invention is:
The present invention adds in conventional medium rare-earth compound Lanthanum (III) nitrate, neodymium nitrate, samaric nitrate, low concentration
Lanthanum (III) nitrate, neodymium nitrate, samaric nitrate can stimulate the meristematic zone of root, promote that tissue cultured seedling is taken root, it is thus achieved that sturdy root system, improve seedling
Transplanting survival rate；The growth of Multiple Buds can also be promoted simultaneously, increase laurustinus tissue cultured seedling and emerge quantity, improve emergence rate, from
And reduce tissue cultured seedling cost, be so conducive to a large amount of outputs of laurustinus.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1
A kind of laurustinus culture medium, including 65 parts of MS basis mother solution, glucose 5 parts, lactoalbumin hydrolysate 5.5 parts, corn germ
Breast 4.5 parts, Tomato juice 4 parts, Sucus Cocois 4 parts, indolebutyric acid 2.75 parts, naphthalene acetic acid 2.5 parts, ABT root-inducing powder 3.75 parts, nitric acid
Lanthanum 8 parts, activated carbon 6.5 parts, 35 parts of water.
Embodiment 2
A kind of laurustinus culture medium, including 60 parts of MS basis mother solution, glucose 4 parts, lactoalbumin hydrolysate 6 parts, corn embryosperm 4
Part, Tomato juice 4 parts, Sucus Cocois 4 parts, indolebutyric acid 2 parts, naphthalene acetic acid 2 parts, ABT root-inducing powder 3 parts, Lanthanum (III) nitrate 3 parts, neodymium nitrate 4
Part, activated carbon 6 parts, 32 parts of water.
When preparing culture medium, it then follows following steps:
1. preparation MS basis mother solution.
2. weigh appropriate ABT root-inducing powder, lactoalbumin hydrolysate, corn embryosperm, glucose and activated carbon, ABT root-inducing powder is fallen
Enter to boil in tap water and stir, after being cooled to room temperature, add lactoalbumin hydrolysate, corn embryosperm, glucose and activated carbon
Until it is standby after Rong Xieing.
3. being cut into small pieces by the Fructus Lycopersici esculenti of clean peeling, be then placed in juice extractor pulverizing, the homogenate obtained is western red
Fructus Kaki juice and peach juice.
4. by mother solution, ABT root-inducing powder, lactoalbumin hydrolysate, corn embryosperm, glucose, activated carbon, Sucus Cocois, Tomato juice,
After indolebutyric acid, naphthalene acetic acid constant volume by a certain percentage, it is dispensed into culture bottle, high temperature sterilize.
5., in superclean bench, rare-earth compound is dissolved in sterilized water filtration sterilization, then uses liquid-transfering gun subpackage
Go out to bottle each described in 4 in the culture medium of bacterium, shaken up.
When preparing culture medium, in addition to various nutrient mother solutions use distilled water preparation, other components all can use certainly
Water is prepared, and can indirectly simplify program and reduce cost.
The technology being not directed in the specific embodiment of the invention belongs to techniques known, adds with reference to known technology
To implement.
Although technical scheme is described in detail by above-described embodiment, but the technical side of the present invention
Case is not limited to above example, in the case of without departing from the thought of the present invention and objective, to technical scheme institute
Any change done falls within claims of the present invention limited range.
Claims (6)
1. a laurustinus special culture media, it is characterised in that: described laurustinus special culture media includes MS basis mother solution 60-65
Part, glucose 4-6 part, lactoalbumin hydrolysate 6-8 part, corn embryosperm 4-8 part, Tomato juice's 4-8 part, Sucus Cocois 2-14 part, indole
Butanoic acid 2-5 part, naphthalene acetic acid 1-4 part, ABT root-inducing powder 2-6 part, rare-earth compound 5-15 part, activated carbon 5-10 part, water 30-
35 parts.
Laurustinus culture medium the most according to claim 1, it is characterised in that: described rare-earth compound be Lanthanum (III) nitrate,
At least one in neodymium nitrate, samaric nitrate.
Laurustinus culture medium the most according to claim 1, it is characterised in that: rare-earth compound adds as follows
To culture medium: in superclean bench environment, the one in Lanthanum (III) nitrate, neodymium nitrate, samaric nitrate or mixture are dissolved in water and are formed
Solution, through filter-sterilizer filtration sterilization, carries out high-temperature sterilization by the culture medium being not added with rare-earth compound simultaneously, in training
Before foster base not yet recovers room temperature, with liquid-transfering gun, the solution of Lanthanum (III) nitrate or neodymium nitrate or samaric nitrate is dispensed into each culture medium
In, and rock uniformly.
Laurustinus culture medium the most according to claim 1, it is characterised in that: described laurustinus special culture media includes MS base
60 parts of plinth mother solution, glucose 4 parts, lactoalbumin hydrolysate 6 parts, corn embryosperm 4 parts, Tomato juice 4 parts, Sucus Cocois 4 parts, indolebutyric acid
2 parts, naphthalene acetic acid 2 parts, ABT root-inducing powder 3 parts, rare-earth compound 7 parts, activated carbon 6 parts, 32 parts of water.
Laurustinus culture medium the most according to claim 4, it is characterised in that described rare-earth compound be Lanthanum (III) nitrate and
The mixture of neodymium nitrate, wherein Lanthanum (III) nitrate is 3:4 with the mass ratio of neodymium nitrate.
Laurustinus culture medium the most according to claim 4, it is characterised in that described rare-earth compound be Lanthanum (III) nitrate,
Neodymium nitrate and the mixture of samaric nitrate, wherein the mass ratio 2:2:3 of Lanthanum (III) nitrate, neodymium nitrate and samaric nitrate.
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| CN201610653568.2A CN106212286B (en) | 2016-08-10 | 2016-08-10 | A kind of laurustinus special culture media |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107980466A (en) * | 2017-12-29 | 2018-05-04 | 福建省林业科学研究院 | A kind of fruit of a cubeb litsea tree tissue-cultured seedling minitype cuttage method |
| CN108040881A (en) * | 2017-12-28 | 2018-05-18 | 广西南宁桂尔创环保科技有限公司 | A kind of dragon fruit synchronization tissue culture culture medium and the cultural method of plant |
| CN109287483A (en) * | 2018-11-02 | 2019-02-01 | 杭州市园林绿化股份有限公司 | A kind of method of bigleaf hydrangea container seedling quickly breeding |
| CN110839529A (en) * | 2019-11-22 | 2020-02-28 | 江苏东郁植物科技有限公司 | Hydrangea liquid culture medium tissue culture rapid propagation method |
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| EP0325816A1 (en) * | 1988-01-05 | 1989-08-02 | Leo Anne Eveleens | Method for forcing hydrangea plants into bloom all year round |
| CN101250497A (en) * | 2008-04-14 | 2008-08-27 | 潍坊学院 | Culture media for plant tissue culture |
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2016
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| EP0325816A1 (en) * | 1988-01-05 | 1989-08-02 | Leo Anne Eveleens | Method for forcing hydrangea plants into bloom all year round |
| CN101250497A (en) * | 2008-04-14 | 2008-08-27 | 潍坊学院 | Culture media for plant tissue culture |
Non-Patent Citations (3)
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| 吴华芬等: "绣球花的植物组织培养和快速繁殖技术", 《浙江农业科学》 * |
| 崔德才等: "《植物组织培养与工厂化育苗》", 31 May 2003 * |
| 江奕凤: "绣球花的组织培养（简报）", 《亚热带植物通讯》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108040881A (en) * | 2017-12-28 | 2018-05-18 | 广西南宁桂尔创环保科技有限公司 | A kind of dragon fruit synchronization tissue culture culture medium and the cultural method of plant |
| CN107980466A (en) * | 2017-12-29 | 2018-05-04 | 福建省林业科学研究院 | A kind of fruit of a cubeb litsea tree tissue-cultured seedling minitype cuttage method |
| CN109287483A (en) * | 2018-11-02 | 2019-02-01 | 杭州市园林绿化股份有限公司 | A kind of method of bigleaf hydrangea container seedling quickly breeding |
| CN109287483B (en) * | 2018-11-02 | 2022-03-25 | 杭州市园林绿化股份有限公司 | Method for quickly cultivating hydrangea macrophylla container seedlings |
| CN110839529A (en) * | 2019-11-22 | 2020-02-28 | 江苏东郁植物科技有限公司 | Hydrangea liquid culture medium tissue culture rapid propagation method |
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| CN106212286B (en) | 2019-06-25 |
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