CN102835315A - Culture medium for cultivating papaya tissue - Google Patents
Culture medium for cultivating papaya tissue Download PDFInfo
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- CN102835315A CN102835315A CN2012103634387A CN201210363438A CN102835315A CN 102835315 A CN102835315 A CN 102835315A CN 2012103634387 A CN2012103634387 A CN 2012103634387A CN 201210363438 A CN201210363438 A CN 201210363438A CN 102835315 A CN102835315 A CN 102835315A
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Abstract
The invention discloses a culture medium for cultivating papaya tissue. The culture medium comprises a subculture medium, a first-step rooting medium and a second-step rooting medium; the combination of a basic culture medium (MS) and a growth regulator is generally adopted on selection of the culture medium, and the emphases are different. Therefore, the culture medium has the advantages that: the subculture cycle is shortened; the quality of buds is good, and the obtained seedling is high in rooting percentage, strong in rooting ability, and fewer in callus and has bottle-green leaves.
Description
Technical field
The present invention relates to tissue culture medium (TCM), relate in particular to a kind of papaya papaw culture medium for tissue culture.
Background technology
Papaya papaw (Carica papaya L.) is one of the famous torrid zone, subtropical fruits; It is a kind of perennial evergreen fruit tree crop that is distributed widely in the torrid zone, the world, subtropical zone; Its because of grow fast, the result early, output is high; Become one of fruit tree kind of the common cultivated in south China and torrid areas, the world, extensively plant on China Guangdong, Guangxi, Fujian, Taiwan and other places.It has important edible and industrial value, is one of important promotion project and tropical agriculture industry restructuring project of the tropical high-efficiency agriculture of current development.Being of high nutritive value of papaya papaw contains soluble solid 12%, and every 100g pulp contains vitamin A, B1, B2, C and G 60-122mg.In the milk of underdone Chinese olive, contain a large amount of " Fructus Chaenomelis ferment (papain) ", as meat soft tender dose can be aid digestion.Purposes is wide, remove mellow fruit and supply to eat raw as fruit, Chinese olive as vegetables edible outside, also can process pickled or process preserved fruit, preserved fruit, jam and fruit juice etc.Mellow fruit also can be made the stomach medicine, and underdone fruit contains abundant papain, can be used to softening meat, helps digest; Pharmaceutically can be used as manufacturing albumen arteries and veins; On beverage industry, can make fining agent; In cosmetic industry, can be used to make face cream, shampoo etc.Green fruit and fleshy root also can be used as feed, and visible the present invention can not only promote the production of papaya papaw, and will greatly stimulate the development of related industries such as food processing, medical and health, beauty and health care and aquaculture, has important economic implications.
Papaya papaw strain property is complicated, has male, female and 3 kinds of plant types of both sexes strain, must plantation both sexes strain in the production as fruit, and as papain production with female plant for well.Papaya papaw is slower with the plant sex performance of seed growing, does not also have quick, easy and effective early stage authentication method at present, has largely influenced production with the seed growing seedling.In addition, the seed price of high-quality papaya papaw is very expensive, and storage tolerance not, and the easy infection damage by disease and insect adopts conventional vegetative propagation technique survival rate such as cuttage, grafting low again, is not suitable for the quick breeding of papaya papaw.Therefore, breeding the consistent good seed of papaya papaw strain property fast through tissue culture technology receives much attention.Big quantity research shows that it is very desirable approach that papaya papaw utilizes group culturation rapid propagating technology to grow seedlings, and has the genetic stability that keeps improved seeds; Produce the advantage of anosis seedling; Characteristics such as sapling multiplication is rapid.
At present, domestic existing many papaya technical research reports also begin to take shape in production application.But papaya papaw is various in style, and different kinds is different to the requirement of medium, and general batch production is produced and produced several kinds simultaneously, if a kind is used a kind of medium, the production operation difficulty is big, and cost is high, is unfavorable for large-scale production.At present the subculture medium emphasis is the bud differentiation, but the bud (being effective bud) that can be used for root induction seldom, and the quality of bud is also bad, thin and delicate, short, Huang Ye; The culture of rootage based formulas is a lot, but it is irregular to go out root, instability, and the root system callusization, quantity is few etc., owing to there is above problem, the papaya seedling cost is high, relatively difficulty is produced in batch production.The general combination of adopting minimal medium (MS) and growth regulator in the selection of medium, minimal medium is generally established 3 kinds of 1/2MS, MS, 3/2MS etc., and growth regulator is selected two kinds of IBA (indolebutyric acid) and NAA (methyl) for use; But the emphasis of subculture medium and root media is different, and as not distinguishing, the seedling rooting rate that obtains is low; Root is expanded; Callus is many, and the blade chrysanthemum etc., exist a lot of not enough.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art, a kind of papaya papaw culture medium for tissue culture is provided, this medium comprises subculture medium, first step root media and the second step root media, wherein:
Component and each components contents of every liter of (L) subculture medium are following:
(1) macroelement: potassium nitrate 2550mg/L, ammonium nitrate 2148mg/L, epsom salt 475mg/L, potassium dihydrogen phosphate 325mg/L, calcium chloride 500mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: 6-benzyladenine 0.35-0.45mg/L, methyl 0.15-0.25mg/L;
Surplus is a distilled water.
Component and each components contents of every liter of (L) first step root media are following:
(1) macroelement: potassium nitrate 2100mg/L, ammonium nitrate 1824mg/L, epsom salt 352mg/L, potassium dihydrogen phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: methyl 0.1-0.2mg/L, indolebutyric acid 1.0-2.0mg/L;
(5) other additives: active carbon 2.0-3.0mg/L;
Surplus is a distilled water.
Every liter (L) second step component and each components contents of root media is following:
(1) macroelement: potassium nitrate 1050mg/L, ammonium nitrate 912mg/L, epsom salt 176mg/L, potassium dihydrogen phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: indolebutyric acid 0.1-0.2mg/L;
(5) other additives: active carbon 3.0-4.0mg/L;
Surplus is a distilled water.
The compound method of the subculture medium in the papaya papaw medium of the present invention is:
(1) preparation macroelement mother liquor, micro-mother liquor, organic matter mother liquor, plant growth regulation mother liquor;
(2) measure various mother liquors successively according to the medium amount of preparation, promptly mix mother liquor;
(3) weighing white sugar and agar, white sugar 30g/L wherein, agar 3.0g/L;
(4) heating white sugar and agar are to dissolving fully;
(5) add measured mixing mother liquor then, the water constant volume stirs;
(6) with the pH value to 5.8 of NaOH and HCl debugging medium;
(7) medium that mixes up pH value divides while hot and installs to blake bottle, and capped is tightened;
(8) put into the autoclave sterilization pot to medium, at 121.6 ℃, 0.1MPa sterilized 20 minutes down;
(9) culture medium after sterilization leaves standstill to be cooled to and solidifies, and the medium of this moment can use.
The compound method of the first step root media in the papaya papaw culture medium for tissue culture of the present invention is:
((1) preparation macroelement mother liquor, micro-mother liquor, organic matter mother liquor, plant growth regulation mother liquor, active carbon;
(2) measure various mother liquors successively according to the medium amount of preparation, promptly mix mother liquor;
(3) weighing white sugar and agar, white sugar 15g/L wherein, agar 3.2g/L;
(4) heating white sugar and agar are to dissolving fully;
(5) add measured mixing mother liquor then, the water constant volume stirs;
(6) with the pH value to 5.8 of NaOH and HCl debugging medium;
(7) medium that mixes up pH value divides while hot and installs to blake bottle, and capped is tightened;
(8) put into the autoclave sterilization pot to medium, at 121.6 ℃, 0.1MPa sterilized 20 minutes down;
(9) culture medium after sterilization leaves standstill to be cooled to and solidifies, and the medium of this moment can use.
In the papaya papaw culture medium for tissue culture of the present invention second step compound method of root media is:
(1) preparation macroelement mother liquor, micro-mother liquor, organic matter mother liquor, active carbon;
(2) measure various mother liquors successively according to the medium amount of preparation, promptly mix mother liquor;
(3) weighing white sugar and agar, white sugar 15g/L wherein, agar 3.4g/L;
(4) heating white sugar and agar are to dissolving fully;
(5) add measured mixing mother liquor then, the water constant volume stirs;
(6) with the pH value to 5.8 of NaOH and HCl debugging medium;
(7) medium that mixes up pH value divides while hot and installs to culture bag, fixes with tripod, clamps;
(8) put into the autoclave sterilization pot to medium, at 121.6 ℃, 0.1MPa sterilized 17 minutes down;
(9) culture medium after sterilization leaves standstill to be cooled to and solidifies, and the medium of this moment can use.
The method of successive transfer culture is in the papaya papaw culture medium for tissue culture of the present invention:
(1) the first low light level was cultivated 7 to 8 days, and subculture seedling wound healing very, differentiates lateral bud on the terminal bud, and nursery stock recovers growing way;
(2) high light was cultivated 8 to 12 days then, and the terminal bud lateral bud is high, and blade is unfolded more than 3 centimetres, the stem semi-lignified.The bud of this moment promptly can be used for culture of rootage.Cultivation temperature remains on 25-30 ℃.
The method of papaya papaw medium first step culture of rootage of the present invention is:
(1) secretly cultivate 5 to 7 days, the seedling base portion of taking root expands, and forms the root original hase.Temperature requirement is more extensive, is not less than 15 ℃ and gets final product, optimum temperature 25-30 ℃.
(2) seedling of taking root of this moment changes the root media continuation cultivation of second step over to.
The method of second step of papaya papaw medium culture of rootage of the present invention is:
(1) high light was cultivated 10 to 15 days, and incubated at room temperature gets final product.
(2) take root height of seedling 4-5 centimetre this moment, root system is more than 5, and blade is unfolded, and seedling stem lignification can go out the garden and transplant.
Compare with the papaya papaw tissue culture medium (TCM) of present prior art, advantage of the present invention is:
Embodiment
With embodiment the present invention is described further below, but the present invention is not limited to these embodiment.
Embodiment 1:
Papaya papaw culture medium for tissue culture, this medium comprise subculture medium, first step root media and the second step root media, wherein:
Component and each components contents of every liter of (L) subculture medium are following:
(1) macroelement: potassium nitrate 2550mg/L, ammonium nitrate 2148mg/L, epsom salt 475mg/L, potassium dihydrogen phosphate 325mg/L, calcium chloride 500mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: 6-benzyladenine 0.35mg/L, methyl 0.25mg/L;
Surplus is a distilled water.
Component and each components contents of every liter of (L) first step root media are following:
(1) macroelement: potassium nitrate 2100mg/L, ammonium nitrate 1824mg/L, epsom salt 352mg/L, potassium dihydrogen phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: methyl 0.1mg/L, indolebutyric acid 2.0mg/L;
(5) other additives: active carbon 2.0mg/L;
Surplus is a distilled water.
Every liter (L) second step component and each components contents of root media is following:
(1) macroelement: potassium nitrate 1050mg/L, ammonium nitrate 912mg/L, epsom salt 176mg/L, potassium dihydrogen phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: indolebutyric acid 0.1mg/L;
(5) other additives: active carbon 3.0mg/L;
Surplus is a distilled water.
Embodiment 2:
Papaya papaw culture medium for tissue culture, this medium comprise subculture medium, first step root media and the second step root media, wherein:
Component and each components contents of every liter of (L) subculture medium are following:
(1) macroelement: potassium nitrate 2550mg/L, ammonium nitrate 2148mg/L, epsom salt 475mg/L, potassium dihydrogen phosphate 325mg/L, calcium chloride 500mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: 6-benzyladenine 0.35mg/L, methyl 0.15mg/L;
Surplus is a distilled water.
Component and each components contents of every liter of (L) first step root media are following:
(1) macroelement: potassium nitrate 2100mg/L, ammonium nitrate 1824mg/L, epsom salt 352mg/L, potassium dihydrogen phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: methyl 0.2mg/L, indolebutyric acid 1.0mg/L;
(5) other additives: active carbon 3.0mg/L;
Surplus is a distilled water.
Every liter (L) second step component and each components contents of root media is following:
(1) macroelement: potassium nitrate 1050mg/L, ammonium nitrate 912mg/L, epsom salt 176mg/L, potassium dihydrogen phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: indolebutyric acid 0.2mg/L;
(5) other additives: active carbon 4.0mg/L;
Surplus is a distilled water.
Embodiment 3:
Papaya papaw culture medium for tissue culture, this medium comprise subculture medium, first step root media and the second step root media, wherein:
Component and each components contents of every liter of (L) subculture medium are following:
(1) macroelement: potassium nitrate 2550mg/L, ammonium nitrate 2148mg/L, epsom salt 475mg/L, potassium dihydrogen phosphate 325mg/L, calcium chloride 500mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: 6-benzyladenine 0.45mg/L, methyl 0.15mg/L;
Surplus is a distilled water.
Component and each components contents of every liter of (L) first step root media are following:
(1) macroelement: potassium nitrate 2100mg/L, ammonium nitrate 1824mg/L, epsom salt 352mg/L, potassium dihydrogen phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: methyl 0.1mg/L, indolebutyric acid 1.0mg/L;
(5) other additives: active carbon 2.0mg/L;
Surplus is a distilled water.
Every liter (L) second step component and each components contents of root media is following:
(1) macroelement: potassium nitrate 1050mg/L, ammonium nitrate 912mg/L, epsom salt 176mg/L, potassium dihydrogen phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: indolebutyric acid 0.1mg/L;
(5) other additives: active carbon 3.0mg/L;
Surplus is a distilled water.
Embodiment 4:
Papaya papaw culture medium for tissue culture, this medium comprise subculture medium, first step root media and the second step root media, wherein:
Component and each components contents of every liter of (L) subculture medium are following:
(1) macroelement: potassium nitrate 2550mg/L, ammonium nitrate 2148mg/L, epsom salt 475mg/L, potassium dihydrogen phosphate 325mg/L, calcium chloride 500mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: 6-benzyladenine 0.45mg/L, methyl 0.25mg/L;
Surplus is a distilled water.
Component and each components contents of every liter of (L) first step root media are following:
(1) macroelement: potassium nitrate 2100mg/L, ammonium nitrate 1824mg/L, epsom salt 352mg/L, potassium dihydrogen phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: methyl 0.2mg/L, indolebutyric acid 2.0mg/L;
(5) other additives: active carbon 3.0mg/L;
Surplus is a distilled water.
Every liter (L) second step component and each components contents of root media is following:
(1) macroelement: potassium nitrate 1050mg/L, ammonium nitrate 912mg/L, epsom salt 176mg/L, potassium dihydrogen phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: indolebutyric acid 0.2mg/L;
(5) other additives: active carbon 4.0mg/L;
Surplus is a distilled water.
Comparative Examples:
Adopt the combination of 1/2MS+IBA, wherein IBA concentration is 0.3mg/L.
The medium that utilizes the foregoing description 1 and Comparative Examples to process carries out tissue culture to papaya papaw, can find out that from the result of following table the technical indicator of embodiment far surpasses Comparative Examples.
Claims (3)
1. papaya papaw culture medium for tissue culture, this medium comprise subculture medium, first step root media and the second step root media, it is characterized in that:
Component and each components contents of every liter of (L) subculture medium are following:
(1) macroelement: potassium nitrate 2550mg/L, ammonium nitrate 2148mg/L, epsom salt 475mg/L, potassium dihydrogen phosphate 325mg/L, calcium chloride 500mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: 6-benzyladenine 0.35-0.45mg/L, methyl 0.15-0.25mg/L;
Surplus is a distilled water.
2. papaya papaw culture medium for tissue culture according to claim 1 is characterized in that:
Component and each components contents of every liter of (L) first step root media are following:
(1) macroelement: potassium nitrate 2100mg/L, ammonium nitrate 1824mg/L, epsom salt 352mg/L, potassium dihydrogen phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: methyl 0.1-0.2mg/L, indolebutyric acid 1.0-2.0mg/L;
(5) other additives: active carbon 2.0-3.0mg/L;
Surplus is a distilled water.
3. papaya papaw culture medium for tissue culture according to claim 1 is characterized in that:
Every liter (L) second step component and each components contents of root media is following:
(1) macroelement: potassium nitrate 1050mg/L, ammonium nitrate 912mg/L, epsom salt 176mg/L, potassium dihydrogen phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, white vitriol 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, ferrous sulfate heptahydrate 27.8mg/L, KI 0.83mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: indolebutyric acid 0.1-0.2mg/L;
(5) other additives: active carbon 3.0-4.0mg/L;
Surplus is a distilled water.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104686367A (en) * | 2015-03-31 | 2015-06-10 | 桂林市一峰食品有限公司 | Sweet tea tissue culture medium |
| CN104686365A (en) * | 2015-03-31 | 2015-06-10 | 桂林市一峰食品有限公司 | Culturing media for culturing sweet tea tissues |
| CN106900555A (en) * | 2017-03-21 | 2017-06-30 | 钦州市林业科学研究所 | Amomum viosum test tube plant division culture medium and once-seedling forming tissue culture plant division quick-breeding method |
| CN111072422A (en) * | 2020-01-16 | 2020-04-28 | 河南华薯农业科技有限公司 | Special culture medium for ipomoea batatas 32 and preparation method thereof |
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| CN1481674A (en) * | 2002-09-11 | 2004-03-17 | 广州市果树科学研究所 | Method for production of papaya sprout with strain stabilization |
| CN101268758A (en) * | 2008-05-09 | 2008-09-24 | 南京林业大学 | Quick replication method for Xuan pawpaw tissue cultivation |
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| CN1481674A (en) * | 2002-09-11 | 2004-03-17 | 广州市果树科学研究所 | Method for production of papaya sprout with strain stabilization |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686367A (en) * | 2015-03-31 | 2015-06-10 | 桂林市一峰食品有限公司 | Sweet tea tissue culture medium |
| CN104686365A (en) * | 2015-03-31 | 2015-06-10 | 桂林市一峰食品有限公司 | Culturing media for culturing sweet tea tissues |
| CN106900555A (en) * | 2017-03-21 | 2017-06-30 | 钦州市林业科学研究所 | Amomum viosum test tube plant division culture medium and once-seedling forming tissue culture plant division quick-breeding method |
| CN111072422A (en) * | 2020-01-16 | 2020-04-28 | 河南华薯农业科技有限公司 | Special culture medium for ipomoea batatas 32 and preparation method thereof |
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| CN102835315B (en) | 2013-08-28 |
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