CN103947553A - Passion flower tissue culture medium - Google Patents
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Abstract
The invention discloses a passion flower tissue culture medium. The culture medium comprises a subculture medium, a first-step rooting medium and a second-step rooting medium. The culture medium is generally combined by using a basic culture medium (MS) and a growth regulator at different focuses. The passion flower tissue culture medium has the advantages of shortening of the subculture period, good bud quality, high seedling rooting rate, high rooting capacity, small number of calluses, dark green leaves and the like.
Description
Technical field
The present invention relates to tissue culture medium (TCM), relate in particular to a kind of passionflower culture medium for tissue culture.
Background technology
Passionflower (formal name used at school: Passiflora caerulea L.) is the good to eat fruit of a kind of fragrance, there is the good reputation of " king of fruit juice ", for perennial evergreen is climbed bejuco, strain state grace, evergreen all the year round, blade profile is peculiar, and pattern is bright-coloured, smell fragrance, sweet fruit acid is good to eat, fruit fragrance, edible fruit flesh, sweet and sour taste.With rich flavor, fragrance is pleasant.Passionflower originates in Brazil, afterwards in South America, there is plantation South Africa, Southeast Asian countries, Australia and each department, the South Pacific Ocean.Because the fruit shape of passionflower is like egg, fruit juice color and luster as egg yolk and have the various fruits such as pineapple, guava, banana, mango, grass poison, watermelon, smoked plum, sugarcane and peaches and plums local flavor, so have again egg really, the title of passion fruit.Passionflower whole body is all precious, and according to surveying and determination, passionflower fruit contains and exceedes 135 kinds of above aromatic substances, measure according to chemical analysis, in passionfruit, vitamin A, B, C rich content, also contain phosphorus, calcium, potassium, iron and 17 seed amino acids, go through the compound of 5 kinds, and nutrition and medical value are very high.It is whole, root, leaf can be used as medicine, and has anti-inflammatory, pain relieving, invigorates blood circulation and the effect such as keep fit, lipopenicillinase is hypotensive, and pericarp can be processed into feed.
In dahlia fruit juice, there are 165 kinds of compounds, are suitable for being processed into fruit juice most, or become blended fruit juice with other fruit (as mango, pineapple, guava, mandarin orange orange and apple etc.) processing mixture, can improve significantly mouthfeel and the fragrance of these fruit syrup; Dahlia fruit juice forms peat-reek, has skin maintenance, removes tired, anti-aging, antihypertensive curative effect.There is very high nutritive value, economic worth and ornamental value.
Passionflower is slower with the plant Sex of seed growing, has largely affected production with seed growing seedling.In addition, adopt the conventional vegetative propagation technique survival rate such as cuttage, grafting low again, be not suitable for the Fast-propagation of passionflower.Therefore, receive much attention by the consistent good seed of tissue culture technology Fast-propagation passionflower strain.Quantity research shows greatly, and it is ideal approach that passionflower utilizes group culturation rapid propagating technology to grow seedlings, and has the genetic stability that keeps improved seeds; Produce the advantage of disease-free seedlings; The features such as sapling multiplication is rapid.
At present, domestic existing many passionflower tissue culture technology research reports also begin to take shape in production application.But Characteristics of Passionfruit Cultivars is various, the requirement difference of different kinds to medium, general batch production is produced and is produced several kinds simultaneously, if a kind is used a kind of medium, production operation difficulty is large, cost is high, is unfavorable for large-scale production.At present subculture medium emphasis is bud differentiation, but it is little to be used for the bud (being effective bud) of root induction, and the quality of bud is also bad, thin and delicate, short, Huang Ye; Prescription of rooting medium is a lot, but it is irregular, unstable to go out root, root system callus, and quantity is few etc., and owing to there being above problem, passionflower tissue culture cost is high, batch production comparision of production difficulty.The general combination that adopts minimal medium (MS) and growth regulator in the selection of medium, minimal medium is generally established 3 kinds of 1/2MS, MS, 3/2MS etc., growth regulator is selected two kinds of IBA (indolebutyric acid) and NAA (methyl α-naphthyl acetate), but the emphasis of subculture medium and root media is different, if do not distinguished, the seedling rooting rate obtaining is low, root is expanded, callus is many, and blade chrysanthemum etc., exist a lot of not enough.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of passionflower culture medium for tissue culture is provided, this medium comprises subculture medium, first step root media and second step root media, wherein:
The component of every liter of (L) subculture medium and the content of each component are as follows:
(1) macroelement: potassium nitrate 2450mg/L, ammonium nitrate 2100mg/L, epsom salt 450mg/L, potassium dihydrogen phosphate 350mg/L, calcium chloride 450mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: 6-benzyladenine 0.25-0.35mg/L, methyl α-naphthyl acetate 0.15-0.25mg/L;
Surplus is distilled water.
The component of every liter of (L) first step root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 2100mg/L, ammonium nitrate 1824mg/L, epsom salt 352mg/L, potassium dihydrogen phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: methyl α-naphthyl acetate 0.1-0.2mg/L, indolebutyric acid 1.5-2.5mg/L;
(5) other additives: active carbon 2.0-3.0mg/L;
Surplus is distilled water.
The component of every liter of (L) second step root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 1050mg/L, ammonium nitrate 912mg/L, epsom salt 176mg/L, potassium dihydrogen phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: indolebutyric acid 0.1-0.2mg/L;
(5) other additives: active carbon 3.0-4.0mg/L;
Surplus is distilled water.
The compound method of the subculture medium in passionflower medium of the present invention is:
(1) preparation macroelement mother liquor, micro-mother liquor, organic matter mother liquor, plant growth regulation mother liquor;
(2) measure successively various mother liquors according to medium amount of preparation, mix mother liquor;
(3) weigh white sugar and agar, wherein white sugar 30g/L, agar 3.0g/L;
(4) heating white sugar and agar are to dissolving completely;
(5) then add measured mixing mother liquor, water constant volume, stirs;
(6) use NaOH and HCl to debug the pH value to 5.8 of medium;
(7) medium that mixes up pH value divides while hot and installs to blake bottle, covers bottle cap, tightens;
(8) medium is put into autoclave sterilization pot, at 121.6 DEG C, sterilizing 20 minutes under 0.1MPa;
(9) medium after sterilizing leaves standstill to be cooled to and solidifies, and medium now can use.
The compound method of the first step root media in passionflower culture medium for tissue culture of the present invention is:
(1) preparation macroelement mother liquor, micro-mother liquor, organic matter mother liquor, plant growth regulation mother liquor, active carbon;
(2) measure successively various mother liquors according to medium amount of preparation, mix mother liquor;
(3) weigh white sugar and agar, wherein white sugar 15g/L, agar 3.2g/L;
(4) heating white sugar and agar are to dissolving completely;
(5) then add measured mixing mother liquor, water constant volume, stirs;
(6) use NaOH and HCl to debug the pH value to 5.8 of medium;
(7) medium that mixes up pH value divides while hot and installs to blake bottle, covers bottle cap, tightens;
(8) medium is put into autoclave sterilization pot, at 121.6 DEG C, sterilizing 20 minutes under 0.1MPa;
(9) medium after sterilizing leaves standstill to be cooled to and solidifies, and medium now can use.
The compound method of the second step root media in passionflower culture medium for tissue culture of the present invention is:
(1) preparation macroelement mother liquor, micro-mother liquor, organic matter mother liquor, active carbon;
(2) measure successively various mother liquors according to medium amount of preparation, mix mother liquor;
(3) weigh white sugar and agar, wherein white sugar 15g/L, agar 3.4g/L;
(4) heating white sugar and agar are to dissolving completely;
(5) then add measured mixing mother liquor, water constant volume, stirs;
(6) use NaOH and HCl to debug the pH value to 5.8 of medium;
(7) medium that mixes up pH value divides while hot and installs to culture bag, fixes with tripod, clamps;
(8) medium is put into autoclave sterilization pot, at 121.6 DEG C, sterilizing 17 minutes under 0.1MPa;
(9) medium after sterilizing leaves standstill to be cooled to and solidifies, and medium now can use.
The method of passionflower culture medium for tissue culture relaying culture of the present invention is:
(1) the first low light level is cultivated 7 to 8 days, and subculture seedling wound healing very, differentiates lateral bud on terminal bud, and nursery stock recovers growing way;
(2) then high light is cultivated 8 to 12 days, and terminal bud lateral bud is high, and more than 3 centimetres blade is unfolded, stem semi-lignified.Bud now can be used for culture of rootage.Cultivation temperature remains on 25-30 DEG C.
The method of passionflower medium first step culture of rootage of the present invention is:
(1) secretly cultivate 5 to 7 days, the seedling base portion of taking root expands, and forms the former base of root.Temperature requirement is more extensive, is not less than 15 DEG C, optimum temperature 25-30 DEG C.
(2) seedling of taking root now proceeds to second step root media and continues to cultivate.
The method of passionflower medium second step culture of rootage of the present invention is:
(1) high light is cultivated 10 to 15 days, incubated at room temperature.
(2) the height of seedling 4-5 centimetre of now taking root, root system is more than 5, and blade is unfolded, and seedling stem lignification can go out garden and transplant.
Compared with the passionflower tissue culture medium (TCM) of currently available technology, advantage of the present invention is:
Embodiment
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiment.
Embodiment 1:
Passionflower culture medium for tissue culture, this medium comprises subculture medium, first step root media and second step root media, wherein:
The component of every liter of (L) subculture medium and the content of each component are as follows:
(1) macroelement: potassium nitrate 2450mg/L, ammonium nitrate 2100mg/L, epsom salt 450mg/L, potassium dihydrogen phosphate 350mg/L, calcium chloride 450mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: 6-benzyladenine 0.35mg/L, methyl α-naphthyl acetate 0.25mg/L;
Surplus is distilled water.
The component of every liter of (L) first step root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 2100mg/L, ammonium nitrate 1824mg/L, epsom salt 352mg/L, potassium dihydrogen phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: methyl α-naphthyl acetate 0.1mg/L, indolebutyric acid 2.0mg/L;
(5) other additives: active carbon 2.0mg/L;
Surplus is distilled water.
The component of every liter of (L) second step root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 1050mg/L, ammonium nitrate 912mg/L, epsom salt 176mg/L, potassium dihydrogen phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: indolebutyric acid 0.1mg/L;
(5) other additives: active carbon 3.0mg/L;
Surplus is distilled water.
Embodiment 2:
Passionflower culture medium for tissue culture, this medium comprises subculture medium, first step root media and second step root media, wherein:
The component of every liter of (L) subculture medium and the content of each component are as follows:
(1) macroelement: potassium nitrate 2450mg/L, ammonium nitrate 2100mg/L, epsom salt 450mg/L, potassium dihydrogen phosphate 350mg/L, calcium chloride 450mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: 6-benzyladenine 0.25mg/L, methyl α-naphthyl acetate 0.15mg/L;
Surplus is distilled water.
The component of every liter of (L) first step root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 2100mg/L, ammonium nitrate 1824mg/L, epsom salt 352mg/L, potassium dihydrogen phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: methyl α-naphthyl acetate 0.2mg/L, indolebutyric acid 1.5mg/L;
(5) other additives: active carbon 3.0mg/L;
Surplus is distilled water.
The component of every liter of (L) second step root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 1050mg/L, ammonium nitrate 912mg/L, epsom salt 176mg/L, potassium dihydrogen phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: indolebutyric acid 0.2mg/L;
(5) other additives: active carbon 4.0mg/L;
Surplus is distilled water.
Embodiment 3:
Passionflower culture medium for tissue culture, this medium comprises subculture medium, first step root media and second step root media, wherein:
The component of every liter of (L) subculture medium and the content of each component are as follows:
(1) macroelement: potassium nitrate 2450mg/L, ammonium nitrate 2100mg/L, epsom salt 450mg/L, potassium dihydrogen phosphate 350mg/L, calcium chloride 450mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: 6-benzyladenine 0.30mg/L, methyl α-naphthyl acetate 0.15mg/L;
Surplus is distilled water.
The component of every liter of (L) first step root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 2100mg/L, ammonium nitrate 1824mg/L, epsom salt 352mg/L, potassium dihydrogen phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: methyl α-naphthyl acetate 0.1mg/L, indolebutyric acid 2.5mg/L;
(5) other additives: active carbon 2.0mg/L;
Surplus is distilled water.
The component of every liter of (L) second step root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 1050mg/L, ammonium nitrate 912mg/L, epsom salt 176mg/L, potassium dihydrogen phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: indolebutyric acid 0.1mg/L;
(5) other additives: active carbon 3.0mg/L;
Surplus is distilled water.
Embodiment 4:
Passionflower culture medium for tissue culture, this medium comprises subculture medium, first step root media and second step root media, wherein:
The component of every liter of (L) subculture medium and the content of each component are as follows:
(1) macroelement: potassium nitrate 2450mg/L, ammonium nitrate 2100mg/L, epsom salt 450mg/L, potassium dihydrogen phosphate 350mg/L, calcium chloride 450mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: 6-benzyladenine 0.35mg/L, methyl α-naphthyl acetate 0.25mg/L;
Surplus is distilled water.
The component of every liter of (L) first step root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 2100mg/L, ammonium nitrate 1824mg/L, epsom salt 352mg/L, potassium dihydrogen phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: methyl α-naphthyl acetate 0.2mg/L, indolebutyric acid 2.0mg/L;
(5) other additives: active carbon 3.0mg/L;
Surplus is distilled water.
The component of every liter of (L) second step root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 1050mg/L, ammonium nitrate 912mg/L, epsom salt 176mg/L, potassium dihydrogen phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: indolebutyric acid 0.2mg/L;
(5) other additives: active carbon 4.0mg/L;
Surplus is distilled water.
Comparative example:
Adopt the combination of 1/2MS+IBA, wherein IBA concentration is 0.3mg/L.
The medium that utilizes above-described embodiment 1 and comparative example to make, organizes cultivation to passionflower, can find out from the result of following table, and the technical indicator of embodiment far exceedes comparative example.
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Claims (1)
1. a passionflower culture medium for tissue culture, this medium comprises subculture medium, first step root media and second step root media, it is characterized in that:
The component of every liter of (L) subculture medium and the content of each component are as follows:
(1) macroelement: potassium nitrate 2450mg/L, ammonium nitrate 2100mg/L, epsom salt 450mg/L, potassium dihydrogen phosphate 350mg/L, calcium chloride 450mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: 6-benzyladenine 0.25-0.35mg/L, methyl α-naphthyl acetate 0.15-0.25mg/L;
Surplus is distilled water;
The component of every liter of (L) first step root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 2100mg/L, ammonium nitrate 1824mg/L, epsom salt 352mg/L, potassium dihydrogen phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: methyl α-naphthyl acetate 0.1-0.2mg/L, indolebutyric acid 1.5-2.5mg/L;
(5) other additives: active carbon 2.0-3.0mg/L;
Surplus is distilled water;
The component of every liter of (L) second step root media and the content of each component are as follows:
(1) macroelement: potassium nitrate 1050mg/L, ammonium nitrate 912mg/L, epsom salt 176mg/L, potassium dihydrogen phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.0mg/L, white vitriol 8.2mg/L, boric acid 5.5mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 6H2O 0.025mg/L, sodium salt 35.5mg/L, ferrous sulfate heptahydrate 26.5mg/L, potassium iodide 0.75mg/L;
(3) organic matter: glycine 2.0mg/L, thiamine hydrochloride 10.0mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant growth regulator: indolebutyric acid 0.1-0.2mg/L;
(5) other additives: active carbon 3.0-4.0mg/L;
Surplus is distilled water.
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| CN105210864A (en) * | 2015-09-10 | 2016-01-06 | 宋立胜 | A kind of acquisition methods of dahlia detoxic seedling |
| CN105340744A (en) * | 2015-11-17 | 2016-02-24 | 浦北南国水果种植农民专业合作社 | Passion fruit tissue culture seedling method |
| CN114027197A (en) * | 2021-12-09 | 2022-02-11 | 广西大学 | Application of passion fruit tissue culture medium |
| CN114938807A (en) * | 2022-05-06 | 2022-08-26 | 中国热带农业科学院海口实验站 | Method for preparing chromosome specimen of root tip meristematic region of passionfruit subgenus plant |
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| CN103493707A (en) * | 2013-08-29 | 2014-01-08 | 高深 | Passion fruit planting method |
| CN103733934A (en) * | 2013-12-26 | 2014-04-23 | 广西恒得润生物科技有限公司 | Cultivation method of passion fruits |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105210864A (en) * | 2015-09-10 | 2016-01-06 | 宋立胜 | A kind of acquisition methods of dahlia detoxic seedling |
| CN105340744A (en) * | 2015-11-17 | 2016-02-24 | 浦北南国水果种植农民专业合作社 | Passion fruit tissue culture seedling method |
| CN114027197A (en) * | 2021-12-09 | 2022-02-11 | 广西大学 | Application of passion fruit tissue culture medium |
| CN114938807A (en) * | 2022-05-06 | 2022-08-26 | 中国热带农业科学院海口实验站 | Method for preparing chromosome specimen of root tip meristematic region of passionfruit subgenus plant |
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