CN102835315B - Culture medium for cultivating papaya tissue - Google Patents
Culture medium for cultivating papaya tissue Download PDFInfo
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Abstract
The invention discloses a culture medium for cultivating papaya tissue. The culture medium comprises a subculture medium, a first-step rooting medium and a second-step rooting medium; the combination of a basic culture medium (MS) and a growth regulator is generally adopted on selection of the culture medium, and the emphases are different. Therefore, the culture medium has the advantages that: the subculture cycle is shortened; the quality of buds is good, and the obtained seedling is high in rooting percentage, strong in rooting ability, and fewer in callus and has bottle-green leaves.
Description
Technical field
The present invention relates to tissue culture medium (TCM), relate in particular to a kind of papaya culture medium for tissue culture.
Background technology
Papaya (Carica papaya L.) is one of the famous torrid zone, subtropical fruit, it is a kind of perennial evergreen fruit tree crop that is distributed widely in the torrid zone, the world, subtropical zone, its because of grow fast, the result early, the output height, become one of fruit tree kind of the common cultivated in south China and torrid areas, the world, extensively plant on China Guangdong, Guangxi, Fujian, Taiwan and other places.It has important edible and industrial value, is one of the important promotion project of the tropical high-efficiency agriculture of current development and tropical agriculture industry restructuring project.Being of high nutritive value of papaya contains soluble solid 12%, and every 100g pulp contains vitamin A, B1, B2, C and G 60-122mg.In the milk of underdone Chinese olive, contain a large amount of " Fructus Chaenomelis ferment (papain) ", as meat soft tender dose can be aid digestion.Purposes is wide, except mellow fruit as fruit for eating raw, outside Chinese olive is edible as vegetables, also can processes pickled or make preserved fruit, preserved fruit, jam and fruit juice etc.Mellow fruit also can be made the stomach medicine, and underdone fruit contains abundant papoid, can be used to softening meat, helps digest; Pharmaceutically can be used as manufacturing albumen arteries and veins; On drink industry, can make finings; At industrial can be used to make face cream, the shampoo etc. of making up.Green fruit and fleshy root also can be used as feed, and visible the present invention can not only promote the production of papaya, and will greatly stimulate the development of related industrieies such as food-processing, medical and health, beauty and health care and aquaculture, has important economic implications.
Papaya strain complexity has male, female and 3 kinds of plant types of both sexes strain, must plantation both sexes strain as fruit in the production, and as papain production with female plant for well.Papaya is slower with the plant sex performance of seed growing, does not also have quick, easy and effective early stage authentication method at present, has largely influenced production with the seed growing seedling.In addition, the seed price of high-quality papaya is very expensive, and storage tolerance not, and the easy infection disease and pest adopts conventional vegetative propagation technique surviving rate such as cuttage, grafting low again, is not suitable for the quick breeding of papaya.Therefore, the good seed of breeding papaya strain unanimity fast by tissue culture technique receives much attention.Studies show that in a large number it is very desirable approach that papaya utilizes group culturation rapid propagating technology to grow seedlings, have the genetic stability that keeps improved seeds; Produce the advantage of anosis seedling; Characteristics such as sapling multiplication is rapid.
At present, domestic existing many papaya technical study reports also begin to take shape in production application.But papaya is various in style, and different kinds is to the requirement difference of substratum, and general batch production is produced and produced several kinds simultaneously, if a kind is used a kind of substratum, the production operation difficulty is big, and the cost height is unfavorable for large-scale production.At present the subculture medium emphasis is the bud differentiation, but can be for the bud (being effective bud) of root induction seldom, and the quality of bud is also bad, thin and delicate, short, Huang Ye; The root culture based formulas is a lot, but it is irregular to go out root, instability, and the root system callusization, quantity is few etc., owing to have above problem, and papaya seedling cost height, relatively difficulty is produced in batch production.The general combination of adopting minimum medium (MS) and growth regulator in the selection of substratum, minimum medium is generally established 3 kinds of 1/2MS, MS, 3/2MS etc., growth regulator is selected two kinds of IBA (indolebutyric acid) and NAA (naphthylacetic acid) for use, but the special emphasis of subculture medium and root media is different, and as not distinguishing, the seedling rooting rate that obtains is low, root is expanded, callus is many, and the blade chrysanthemum etc., exist a lot of not enough.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of papaya culture medium for tissue culture is provided, this substratum comprises subculture medium, the first step root media and the second step root media, wherein:
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 2550mg/L, ammonium nitrate 2148mg/L, magnesium sulfate heptahydrate 475mg/L, potassium primary phosphate 325mg/L, calcium chloride 500mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: 6-benzyladenine 0.35-0.45mg/L, naphthylacetic acid 0.15-0.25mg/L;
Surplus is distilled water.
Component and each components contents of every liter of (L) the first step root media are as follows:
(1) macroelement: saltpetre 2100mg/L, ammonium nitrate 1824mg/L, magnesium sulfate heptahydrate 352mg/L, potassium primary phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.1-0.2mg/L, indolebutyric acid 1.0-2.0mg/L;
(5) other additives: gac 2.0-3.0mg/L;
Surplus is distilled water.
Every liter (L) second step component and each components contents of root media is as follows:
(1) macroelement: saltpetre 1050mg/L, ammonium nitrate 912mg/L, magnesium sulfate heptahydrate 176mg/L, potassium primary phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: indolebutyric acid 0.1-0.2mg/L;
(5) other additives: gac 3.0-4.0mg/L;
Surplus is distilled water.
The compound method of the subculture medium in the papaya substratum of the present invention is:
(1) preparation macroelement mother liquor, micro-mother liquor, organism mother liquor, plant growth regulation mother liquor;
(2) measure various mother liquors successively according to the substratum amount of preparation, namely mix mother liquor;
(3) weighing white sugar and agar, white sugar 30g/L wherein, agar 3.0g/L;
(4) heating white sugar and agar are to dissolving fully;
(5) add measured mixing mother liquor then, the water constant volume stirs;
(6) use NaOH and HCl to debug the pH value to 5.8 of substratum;
(7) substratum that mixes up pH value divides while hot and installs to culturing bottle, covers bottle cap, tightens;
(8) substratum is put into the autoclave sterilization pot, at 121.6 ℃, sterilization is 20 minutes under the 0.1MPa;
(9) culture medium after sterilization leaves standstill to be cooled to and solidifies, and the substratum of this moment can use.
The compound method of the first step root media in the papaya culture medium for tissue culture of the present invention is:
((1) preparation macroelement mother liquor, micro-mother liquor, organism mother liquor, plant growth regulation mother liquor, gac;
(2) measure various mother liquors successively according to the substratum amount of preparation, namely mix mother liquor;
(3) weighing white sugar and agar, white sugar 15g/L wherein, agar 3.2g/L;
(4) heating white sugar and agar are to dissolving fully;
(5) add measured mixing mother liquor then, the water constant volume stirs;
(6) use NaOH and HCl to debug the pH value to 5.8 of substratum;
(7) substratum that mixes up pH value divides while hot and installs to culturing bottle, covers bottle cap, tightens;
(8) substratum is put into the autoclave sterilization pot, at 121.6 ℃, sterilization is 20 minutes under the 0.1MPa;
(9) culture medium after sterilization leaves standstill to be cooled to and solidifies, and the substratum of this moment can use.
In the papaya culture medium for tissue culture of the present invention second step compound method of root media is:
(1) preparation macroelement mother liquor, micro-mother liquor, organism mother liquor, gac;
(2) measure various mother liquors successively according to the substratum amount of preparation, namely mix mother liquor;
(3) weighing white sugar and agar, white sugar 15g/L wherein, agar 3.4g/L;
(4) heating white sugar and agar are to dissolving fully;
(5) add measured mixing mother liquor then, the water constant volume stirs;
(6) use NaOH and HCl to debug the pH value to 5.8 of substratum;
(7) substratum that mixes up pH value divides while hot and installs to culture bag, fixes with tripod, clamps;
(8) substratum is put into the autoclave sterilization pot, at 121.6 ℃, sterilization is 17 minutes under the 0.1MPa;
(9) culture medium after sterilization leaves standstill to be cooled to and solidifies, and the substratum of this moment can use.
The method of succeeding transfer culture is in the papaya culture medium for tissue culture of the present invention:
(1) the first low light level was cultivated 7 to 8 days, and subculture seedling wound healing very, differentiates lateral bud on the terminal bud, and nursery stock recovers growing way;
(2) high light was cultivated 8 to 12 days then, and the terminal bud lateral bud is high, and blade is unfolded more than 3 centimetres, the stem semi-lignified.The bud of this moment namely can be used for root culture.Culture temperature remains on 25-30 ℃.
The method of papaya substratum the first step root culture of the present invention is:
(1) secretly cultivate 5 to 7 days, the seedling base portion of taking root expands, and forms the root original hase.Temperature requirement is more extensive, is not less than 15 ℃ and gets final product, optimum temps 25-30 ℃.
(2) Ci Shi the seedling of taking root changes the root media continuation cultivation of second step over to.
The method of second step of papaya substratum root culture of the present invention is:
(1) high light was cultivated 10 to 15 days, and incubated at room temperature gets final product.
(2) take root height of seedling 4-5 centimetre this moment, root system is more than 5, and blade is unfolded, and seedling stem lignifying can go out the garden and transplant.
Compare with the papaya tissue culture medium (TCM) of present prior art, advantage of the present invention is:
Embodiment
The invention will be further described with embodiment below, but the present invention is not limited to these embodiment.
Embodiment 1:
Papaya culture medium for tissue culture, this substratum comprise subculture medium, the first step root media and the second step root media, wherein:
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 2550mg/L, ammonium nitrate 2148mg/L, magnesium sulfate heptahydrate 475mg/L, potassium primary phosphate 325mg/L, calcium chloride 500mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: 6-benzyladenine 0.35mg/L, naphthylacetic acid 0.25mg/L;
Surplus is distilled water.
Component and each components contents of every liter of (L) the first step root media are as follows:
(1) macroelement: saltpetre 2100mg/L, ammonium nitrate 1824mg/L, magnesium sulfate heptahydrate 352mg/L, potassium primary phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.1mg/L, indolebutyric acid 2.0mg/L;
(5) other additives: gac 2.0mg/L;
Surplus is distilled water.
Every liter (L) second step component and each components contents of root media is as follows:
(1) macroelement: saltpetre 1050mg/L, ammonium nitrate 912mg/L, magnesium sulfate heptahydrate 176mg/L, potassium primary phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: indolebutyric acid 0.1mg/L;
(5) other additives: gac 3.0mg/L;
Surplus is distilled water.
Embodiment 2:
Papaya culture medium for tissue culture, this substratum comprise subculture medium, the first step root media and the second step root media, wherein:
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 2550mg/L, ammonium nitrate 2148mg/L, magnesium sulfate heptahydrate 475mg/L, potassium primary phosphate 325mg/L, calcium chloride 500mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: 6-benzyladenine 0.35mg/L, naphthylacetic acid 0.15mg/L;
Surplus is distilled water.
Component and each components contents of every liter of (L) the first step root media are as follows:
(1) macroelement: saltpetre 2100mg/L, ammonium nitrate 1824mg/L, magnesium sulfate heptahydrate 352mg/L, potassium primary phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.2mg/L, indolebutyric acid 1.0mg/L;
(5) other additives: gac 3.0mg/L;
Surplus is distilled water.
Every liter (L) second step component and each components contents of root media is as follows:
(1) macroelement: saltpetre 1050mg/L, ammonium nitrate 912mg/L, magnesium sulfate heptahydrate 176mg/L, potassium primary phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: indolebutyric acid 0.2mg/L;
(5) other additives: gac 4.0mg/L;
Surplus is distilled water.
Embodiment 3:
Papaya culture medium for tissue culture, this substratum comprise subculture medium, the first step root media and the second step root media, wherein:
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 2550mg/L, ammonium nitrate 2148mg/L, magnesium sulfate heptahydrate 475mg/L, potassium primary phosphate 325mg/L, calcium chloride 500mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: 6-benzyladenine 0.45mg/L, naphthylacetic acid 0.15mg/L;
Surplus is distilled water.
Component and each components contents of every liter of (L) the first step root media are as follows:
(1) macroelement: saltpetre 2100mg/L, ammonium nitrate 1824mg/L, magnesium sulfate heptahydrate 352mg/L, potassium primary phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.1mg/L, indolebutyric acid 1.0mg/L;
(5) other additives: gac 2.0mg/L;
Surplus is distilled water.
Every liter (L) second step component and each components contents of root media is as follows:
(1) macroelement: saltpetre 1050mg/L, ammonium nitrate 912mg/L, magnesium sulfate heptahydrate 176mg/L, potassium primary phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: indolebutyric acid 0.1mg/L;
(5) other additives: gac 3.0mg/L;
Surplus is distilled water.
Embodiment 4:
Papaya culture medium for tissue culture, this substratum comprise subculture medium, the first step root media and the second step root media, wherein:
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 2550mg/L, ammonium nitrate 2148mg/L, magnesium sulfate heptahydrate 475mg/L, potassium primary phosphate 325mg/L, calcium chloride 500mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: 6-benzyladenine 0.45mg/L, naphthylacetic acid 0.25mg/L;
Surplus is distilled water.
Component and each components contents of every liter of (L) the first step root media are as follows:
(1) macroelement: saltpetre 2100mg/L, ammonium nitrate 1824mg/L, magnesium sulfate heptahydrate 352mg/L, potassium primary phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.2mg/L, indolebutyric acid 2.0mg/L;
(5) other additives: gac 3.0mg/L;
Surplus is distilled water.
Every liter (L) second step component and each components contents of root media is as follows:
(1) macroelement: saltpetre 1050mg/L, ammonium nitrate 912mg/L, magnesium sulfate heptahydrate 176mg/L, potassium primary phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: indolebutyric acid 0.2mg/L;
(5) other additives: gac 4.0mg/L;
Surplus is distilled water.
Comparative Examples:
Adopt the combination of 1/2MS+IBA, wherein IBA concentration is 0.3mg/L.
The substratum that utilizes above-described embodiment 1 and Comparative Examples to make carries out tissue culture to papaya, and from the result of following table as can be seen, the technical indicator of embodiment far surpasses Comparative Examples.
Claims (1)
1. papaya culture medium for tissue culture, this substratum comprise subculture medium, the first step root media and the second step root media, it is characterized in that:
Component and each components contents of every liter of (L) subculture medium are as follows:
(1) macroelement: saltpetre 2550mg/L, ammonium nitrate 2148mg/L, magnesium sulfate heptahydrate 475mg/L, potassium primary phosphate 325mg/L, calcium chloride 500mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: 6-benzyladenine 0.35-0.45mg/L, naphthylacetic acid 0.15-0.25mg/L;
Surplus is distilled water;
Component and each components contents of every liter of (L) the first step root media are as follows:
(1) macroelement: saltpetre 2100mg/L, ammonium nitrate 1824mg/L, magnesium sulfate heptahydrate 352mg/L, potassium primary phosphate 222mg/L, calcium chloride 490mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: naphthylacetic acid 0.1-0.2mg/L, indolebutyric acid 1.0-2.0mg/L;
(5) other additives: gac 2.0-3.0mg/L;
Surplus is distilled water;
Every liter (L) second step component and each components contents of root media is as follows:
(1) macroelement: saltpetre 1050mg/L, ammonium nitrate 912mg/L, magnesium sulfate heptahydrate 176mg/L, potassium primary phosphate 111mg/L, calcium chloride 245mg/L;
(2) trace element: manganese sulfate monohydrate 22.3mg/L, Zinc Sulphate Heptahydrate 8.6mg/L, boric acid 6.2mg/L, Sodium Molybdate Dihydrate 0.025mg/L, cupric sulfate pentahydrate 0.025mg/L, CoCL2 0.025mg/L, sodium salt 37.3mg/L, iron vitriol 27.8mg/L, potassiumiodide 0.83mg/L;
(3) organism: glycine 2.0mg/L, vitamin 10.0mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, inositol 100.0mg/L;
(4) plant-growth regulator: indolebutyric acid 0.1-0.2mg/L;
(5) other additives: gac 3.0-4.0mg/L;
Surplus is distilled water.
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| CN104686367A (en) * | 2015-03-31 | 2015-06-10 | 桂林市一峰食品有限公司 | Sweet tea tissue culture medium |
| CN106900555B (en) * | 2017-03-21 | 2018-10-09 | 钦州市林业科学研究所 | Amomum viosum test tube plant division culture medium and once-seedling forming tissue culture plant division quick-breeding method |
| CN111072422A (en) * | 2020-01-16 | 2020-04-28 | 河南华薯农业科技有限公司 | Special culture medium for ipomoea batatas 32 and preparation method thereof |
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| CN1481674A (en) * | 2002-09-11 | 2004-03-17 | 广州市果树科学研究所 | Method for production of papaya sprout with strain stabilization |
| CN101268758A (en) * | 2008-05-09 | 2008-09-24 | 南京林业大学 | Quick replication method for Xuan pawpaw tissue cultivation |
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| CN1481674A (en) * | 2002-09-11 | 2004-03-17 | 广州市果树科学研究所 | Method for production of papaya sprout with strain stabilization |
| CN101268758A (en) * | 2008-05-09 | 2008-09-24 | 南京林业大学 | Quick replication method for Xuan pawpaw tissue cultivation |
Non-Patent Citations (7)
| Title |
|---|
| "中白"番木瓜组培苗规模化生产技术;时群等;《吉林农业》;20101231(第10期);第99-100页 * |
| 吕平等.番木瓜组织培养概况.《热带农业科技》.2006,第29卷(第2期),第31-33页. |
| 时群等."中白"番木瓜组培苗规模化生产技术.《吉林农业》.2010,(第10期),第99-100页. |
| 李晓艳，潘丹.番木瓜组织培养无菌体系的建立.《吉林农业》.2010,(第9期),第45页. * |
| 游恺哲等.番木瓜继代培养影响因素的探讨.《福建果树》.2007,(第1期),第5-7页. |
| 番木瓜组织培养概况;吕平等;《热带农业科技》;20061231;第29卷(第2期);第31-33页 * |
| 番木瓜继代培养影响因素的探讨;游恺哲等;《福建果树》;20071231(第1期);第5-7页 * |
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