CN106171988A - Improve blue berry detoxic seedling Fast-propagation survival rate culture medium and preparation method thereof - Google Patents
Improve blue berry detoxic seedling Fast-propagation survival rate culture medium and preparation method thereof Download PDFInfo
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- CN106171988A CN106171988A CN201610551412.3A CN201610551412A CN106171988A CN 106171988 A CN106171988 A CN 106171988A CN 201610551412 A CN201610551412 A CN 201610551412A CN 106171988 A CN106171988 A CN 106171988A
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- culture medium
- blue berry
- survival rate
- detoxic seedling
- preparation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of culture medium, be specifically related to a kind of raising blue berry detoxic seedling Fast-propagation survival rate culture medium and preparation method thereof.Described culture medium, minimal medium is WPM, and it is added with 0.2 1.0g/L lactoalbumin hydrolysate, 0.4 1.2g/L vitamin E, 0.002 0.006g/L zeatin, 0.002 0.008g/L 6 benzyladenine, 0.003 0.01g/L naphthalene acetic acid, 0.004 0.02g/L 2,4 dichlorphenoxyacetic acid and 10 40g/L sucrose.Described culture medium ensure that the blue berry detoxic seedling good growth conditions in tissue-culturing rapid propagation and high survival rate, cultivates making detoxic seedling for blueberry tissue and lays a good foundation；Described preparation method, simple, convenient to operate.
Description
Technical field
The present invention relates to a kind of culture medium, be specifically related to a kind of raising blue berry detoxic seedling Fast-propagation survival rate culture medium
And preparation method thereof.
Background technology
Blue berry is a kind of small berries, fruit in blue, sarcocarp is fine and smooth, seed is minimum, has " king of berry " and " fruit emperor
Good reputation afterwards ".Blueberry averagely weighs 0.5～2.5g, maximum weight 5g, and edible rate is 100%, sweet and sour palatability, and has fragrant refreshing
Pleasant fragrance, for eating good merchantable brand raw.Blue berry is not only rich in conventional nutrients, such as proteins,vitamins,minerals and trace unit
Element etc., but also containing extremely abundant anthocyanidin, flavonoid and polysaccharide compound, there is alleviation asthenopia, promote to regard
Rhodopsin regeneration in retinulae, pre-myopia prevention, improves and promotes the effects such as vision.
Except alleviating in addition to asthenopia, blue berry also has many health cares, such as prevention cardiovascular and cerebrovascular disease, gives protection against cancer, improves
Immunity, alleviate and have sore throat and diarrhoea etc., so, utilization and spin-off exploitation to blue berry increasingly come into one's own.
In view of the broad mass market prospect of blue berry, the research and development to blue berry nursery stock the most increasingly come into one's own.Train blue berry nursery stock
In educating, can be by cuttage and tissue culture detoxicating mode.Cutting seedling owing to may cause a timing containing virus pathogenic bacteria etc.
Blue berry quality deterioration after between, thus affect Yield and quality and the economic benefit of blue berry.Therefore, for preventing blue berry quality deterioration existing
As occurring, it is ensured that the quality of blue berry and yield and the economic benefit of blue berry manufacturing enterprise, carry out numerous by making blue berry detoxic seedling
Educate the first-selected varieties breeding mode just becoming outstanding science-and-technology enterprise or scientific and technical research personnel.
Detoxic seedling makes, and typically chooses blue berry stem apex or leaf bud etc. and has the separate living tissue of stronger meristematic capacity, in reality
Test in room and remove virus treated through sterilizing, be then inoculated in aseptic virus-free culture medium, by progressively cultivating, then observe
The contamination microbiological contamination situation of blue berry Seedling, filters out the blue berry seedling not having virus and bacterial invasion, by modern biotechnology means
Cutting is the most numerous further, thus realizes obtaining the purpose of a large amount of blue berry detoxic seedling.
In blue berry detoxic seedling manufacturing process, how to ensure that the detoxic seedling obtained can fast-growth breeding be that blue berry takes off
The key factor whether poison Seedling is successful.
Summary of the invention
For the deficiencies in the prior art, it is an object of the invention to provide a kind of raising blue berry detoxic seedling Fast-propagation survival rate
By culture medium, it is possible to make the blue berry detoxic seedling good growth conditions of holding during tissue-culturing rapid propagation and high survival rate, for
Blueberry tissue is cultivated making detoxic seedling and is laid the foundation；Present invention simultaneously provides its preparation method.
Raising blue berry detoxic seedling Fast-propagation survival rate culture medium of the present invention, minimal medium is WPM, and adds
Added with 0.2-1.0g/L lactoalbumin hydrolysate, 0.4-1.2g/L vitamin E, 0.002-0.006g/L zeatin, 0.002-0.008g/
L 6-benzyladenine, 0.003-0.01g/L naphthalene acetic acid, 0.004-0.02g/L 2,4 dichlorophenoxyacetic acid and 10-40g/L sugarcane
Sugar.
Described culture medium is preferably: minimal medium is WPM, and is added with 0.5g/L lactoalbumin hydrolysate, 0.6g/L dimension
Raw element E, 0.003g/L zeatin, 0.004g/L 6-benzyladenine, 0.004g/L naphthalene acetic acid, 0.008g/L 2,4 dichloro benzene
Fluoroacetic acid and 20g/L sucrose.
The described preparation method improving blue berry detoxic seedling Fast-propagation survival rate culture medium, by lactoalbumin hydrolysate, dimension
Raw element E, zeatin, 6-benzyladenine, naphthalene acetic acid, 2,4-dichlorphenoxyacetic acid and sucrose join in minimal medium, mixed
Subpackage after closing uniformly, standby after sterilizing.
Described subpackage specification is 20-30mL/ bottle.
Compared with prior art, beneficial effects of the present invention is as follows:
(1), in the present invention, WPM minimal medium provides the growth desired nutritional composition of blueberry tissue culture Seedling, and vitamin
E, lactoalbumin hydrolysate has the function of suppression phenoloxidase activity, and a combination thereof can alleviate tissue cultured seedling metabolite to blue berry Seedling
Toxic action, lactoalbumin hydrolysate can also supply nutrient needed for seedling as nutrient；Zeatin, 6-benzyladenine, naphthalene
Acetic acid and 2,4-dichlorphenoxyacetic acid etc. as phytohormone composition, has promotion cell division, promotes root growth, promotes leaf
The functions such as Bud polarization, four Synergistics ensure that the quick division growth of blueberry tissue culture Seedling；Sucrose provide not only blue berry group
Carbon supply needed for training growth of seedling, can regulate Premeabilisation of cells pressure, it is ensured that the normal growth of blue berry bottle Seedling simultaneously.
(2) raising blue berry detoxic seedling Fast-propagation survival rate culture medium of the present invention, it is possible to ensure blue berry detoxification
The Seedling good growth conditions in tissue-culturing rapid propagation and high survival rate, cultivate for blueberry tissue and make detoxic seedling and established base
Plinth.
(3) preparation method of the present invention, simple, convenient to operate.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described.
The minimal medium WPM used in embodiment uses those skilled in the art's well-known formulations to prepare.
Embodiment 1
Described raising blue berry detoxic seedling Fast-propagation survival rate culture medium, minimal medium is WPM, and is added with
0.5g/L lactoalbumin hydrolysate, 0.6g/L vitamin E, 0.003g/L zeatin, 0.004g/L 6-benzyladenine, 0.004g/L
Naphthalene acetic acid, 0.008g/L 2,4 dichlorophenoxyacetic acid and 20g/L sucrose.
Its preparation method is:
By lactoalbumin hydrolysate, vitamin E, zeatin, 6-benzyladenine, naphthalene acetic acid, 2,4 dichlorophenoxyacetic acid and sugarcane
Sugar joins in minimal medium, subpackage after mix homogeneously, standby after sterilizing.
Subpackage specification is 25mL/ bottle.
Embodiment 2
Described raising blue berry detoxic seedling Fast-propagation survival rate culture medium, minimal medium is WPM, and is added with
0.2g/L lactoalbumin hydrolysate, 0.4g/L vitamin E, 0.002g/L zeatin, 0.002g/L 6-benzyladenine, 0.003g/L
Naphthalene acetic acid, 0.004g/L 2,4 dichlorophenoxyacetic acid and 10g/L sucrose.
Its preparation method is:
By lactoalbumin hydrolysate, vitamin E, zeatin, 6-benzyladenine, naphthalene acetic acid, 2,4 dichlorophenoxyacetic acid and sugarcane
Sugar joins in minimal medium, subpackage after mix homogeneously, standby after sterilizing.
Subpackage specification is 20mL/ bottle.
Embodiment 3
Minimal medium is WPM, and is added with 1.0g/L lactoalbumin hydrolysate, 1.2g/L vitamin E, 0.006g/L Semen Maydis
Element, 0.008g/L 6-benzyladenine, 0.01g/L naphthalene acetic acid, 0.02g/L 2,4 dichlorophenoxyacetic acid and 40g/L sucrose.
Its preparation method is:
By lactoalbumin hydrolysate, vitamin E, zeatin, 6-benzyladenine, naphthalene acetic acid, 2,4 dichlorophenoxyacetic acid and sugarcane
Sugar joins in minimal medium, subpackage after mix homogeneously, standby after sterilizing.
Subpackage specification is 30mL/ bottle.
Blue berry Seedling is inoculated into respectively WPM minimal medium and in culture medium prepared by embodiment 1-3, observes after 5 weeks
Blue berry Seedling growing state on different culture media, is specifically shown in Table 1.The situation survival rate of surviving represents.Survival rate computing formula
By: total blue berry Seedling the number inoculated in blue berry Seedling the number/each culture bottle survived in survival rate=each culture bottle.
The growing state of blue berry Seedling on table 1 different culture media
| Every bottle graft kind sum | Survive a number | Survival rate (%) | |
| WPM minimal medium | 30 | 8 | 26.7 |
| Embodiment 1 culture medium | 30 | 30 | 100 |
| Embodiment 2 culture medium | 30 | 26 | 86.7 |
| Embodiment 3 culture medium | 30 | 24 | 80 |
Observe and find: culture medium prepared by embodiment 1-3 can significantly improve the one-tenth of blue berry detoxication and fast reproduction tissue cultured seedling
Motility rate, improves the growth conditions of bottle Seedling.The growing state of the blue berry Seedling in embodiment 1-3 culture medium is far superior to minimal medium
On, wherein the blue berry Seedling growing state in embodiment 1 culture medium is optimum.
Claims (4)
1. one kind is improved blue berry detoxic seedling Fast-propagation survival rate culture medium, it is characterised in that: minimal medium is WPM, and
It is added with 0.2-1.0g/L lactoalbumin hydrolysate, 0.4-1.2g/L vitamin E, 0.002-0.006g/L zeatin, 0.002-
0.008g/L6-benzyladenine, 0.003-0.01g/L naphthalene acetic acid, 0.004-0.02g/L 2,4 dichlorophenoxyacetic acid and 10-
40g/L sucrose.
Raising blue berry detoxic seedling Fast-propagation survival rate culture medium the most according to claim 1, it is characterised in that: basic
Culture medium is WPM, and is added with 0.5g/L lactoalbumin hydrolysate, 0.6g/L vitamin E, 0.003g/L zeatin, 0.004g/L 6-
Benzyladenine, 0.004g/L naphthalene acetic acid, 0.008g/L 2,4 dichlorophenoxyacetic acid and 20g/L sucrose.
3. the preparation method improving blue berry detoxic seedling Fast-propagation survival rate culture medium described in claim 1 or 2, its
It is characterised by: by lactoalbumin hydrolysate, vitamin E, zeatin, 6-benzyladenine, naphthalene acetic acid, 2,4 dichlorophenoxyacetic acid and sugarcane
Sugar joins in minimal medium, subpackage after mix homogeneously, standby after sterilizing.
The preparation method of raising blue berry detoxic seedling Fast-propagation survival rate culture medium the most according to claim 3, it is special
Levy and be: described subpackage specification is 20-30mL/ bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610551412.3A CN106171988B (en) | 2016-07-13 | 2016-07-13 | Improve quick reproductive survival rate culture medium of blueberry detoxic seedling and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610551412.3A CN106171988B (en) | 2016-07-13 | 2016-07-13 | Improve quick reproductive survival rate culture medium of blueberry detoxic seedling and preparation method thereof |
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| Publication Number | Publication Date |
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| CN106171988A true CN106171988A (en) | 2016-12-07 |
| CN106171988B CN106171988B (en) | 2018-06-22 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110432133A (en) * | 2019-08-27 | 2019-11-12 | 吉林农业大学 | A kind of blueberry rapid breeding method |
Citations (4)
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|---|---|---|---|---|
| CN103004754A (en) * | 2012-12-14 | 2013-04-03 | 苏州和美生物科技有限公司 | Composition for preventing browning of plant tissue culture and using method of composition |
| CN103404434A (en) * | 2013-08-05 | 2013-11-27 | 浙江省农业科学院 | Prevention technology for multiple blueberry viruses at early stage |
| CN103583368A (en) * | 2013-11-22 | 2014-02-19 | 西南林业大学 | Construction method for vaccinium ashei tissue culture rapid propagation system |
| CN104823857A (en) * | 2015-05-18 | 2015-08-12 | 江苏农林职业技术学院 | Seedling raising method for promoting ex-vitro rooting of blueberry tissue culture seedlings |
-
2016
- 2016-07-13 CN CN201610551412.3A patent/CN106171988B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004754A (en) * | 2012-12-14 | 2013-04-03 | 苏州和美生物科技有限公司 | Composition for preventing browning of plant tissue culture and using method of composition |
| CN103404434A (en) * | 2013-08-05 | 2013-11-27 | 浙江省农业科学院 | Prevention technology for multiple blueberry viruses at early stage |
| CN103583368A (en) * | 2013-11-22 | 2014-02-19 | 西南林业大学 | Construction method for vaccinium ashei tissue culture rapid propagation system |
| CN104823857A (en) * | 2015-05-18 | 2015-08-12 | 江苏农林职业技术学院 | Seedling raising method for promoting ex-vitro rooting of blueberry tissue culture seedlings |
Non-Patent Citations (2)
| Title |
|---|
| 朱芳明等: "兔眼蓝莓快繁技术研究", 《西南林业大学学报》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110432133A (en) * | 2019-08-27 | 2019-11-12 | 吉林农业大学 | A kind of blueberry rapid breeding method |
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| CN106171988B (en) | 2018-06-22 |
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