CN106167424A - Utilize the method that edible fungi residue is changed into culture medium of edible fungus by fly larvae - Google Patents
Utilize the method that edible fungi residue is changed into culture medium of edible fungus by fly larvae Download PDFInfo
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- CN106167424A CN106167424A CN201610538301.9A CN201610538301A CN106167424A CN 106167424 A CN106167424 A CN 106167424A CN 201610538301 A CN201610538301 A CN 201610538301A CN 106167424 A CN106167424 A CN 106167424A
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- Prior art keywords
- edible
- culture medium
- edible fungi
- fly larvae
- high protein
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/05—Treatments involving invertebrates, e.g. worms, flies or maggots
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention relates to the manufacture method of culture medium of edible fungus, the method that especially with fly larvae, edible fungi residue is changed into culture medium of edible fungus, comprise the steps of, screen after edible fungi residue is shone dry grinding；By edible fungi ground-slag, dried poultrymanure with dry beer distiller grains by weight mixing, after adding EM bacterium stock solution, fish flour and water mixing, sealing and fermenting makes fly larvae culture matrix；Fly larvae culture matrix is placed in cement conversion pool；Accessing fly blow in fly larvae culture medium to cultivate, then entirety is dried, is ground, and makes high protein edible bacterium culture medium substrate；Need to make high protein edible bacterium culture medium with other culture materials by different proportion combination according to edible fungus culturing.This method solving edible fungi residue and cannot recycle problem, the high protein edible bacterium culture medium of preparation reduces the toxigenic capacity of edible fungi, improves weight and the size of edible fungi, existing significant ecological benefits, also has significant economic benefit.
Description
Technical field
The present invention relates to the manufacture method of culture medium of edible fungus, edible fungi residue is changed into edible especially with fly larvae
The method of bacterium culture medium.
Background technology
Edible fungi because it is nutritious, delicious flavour and extremely common people's favor.China is that edible fungi recognizes, utilizes, cultivates
Country the earliest.Within 1988, edible fungi of china total output leaps to the first in the world, and within 2011, national Edible Fungi total amount reaches
2571.7 ten thousand tons, accounting for more than the 70% of whole world total output, the output value, more than 140,000,000,000 yuan, is exported goods and earned foreign currency 24.07 hundred million dollars.Along with food
With the development of bacterium industry, China the most at least produces the dreg of 4,000,000 tons.The traditional method processing these dregs is to abandon or fire
Burn, planting material, animal feed, crops or vegetable fertilizer etc. as other edible fungi, these methods may pollute the environment
Or cause agricultural organic resource waste, do not meet modern agriculture recycling economy development needs, it is therefore desirable to study edible fungi
The method that slag recycles.
During edible fungus culturing, it is necessary that nitrogen source and carbon source, and nitrogen source add main inorganic with rotten generation
Nitrogen is main, and utilization rate is the highest, and adds organic nitrogen and be likely to result in the increase of production cost, and therefore nitrogen source is added and become some
The bottleneck of edible fungi sector development, limits the development of edible fungi sector.
Summary of the invention
For solving the problems referred to above, the present invention provides one both can utilize edible fungi residue, can provide again the profit in nitrogen source
The method that with fly larvae, edible fungi residue is changed into culture medium of edible fungus, concrete technical scheme is:
The method utilizing fly larvae that edible fungi residue changes into culture medium of edible fungus, comprises the steps of
After step 1, edible fungi residue shine dry grinding, with 20 eye mesh screen screenings；
Step 2, by the edible fungi ground-slag after screening, dried poultrymanure and dry beer distiller grains with weight ratio (4～6): (2～4):
The ratio mixing of 2, then according to raw material gross weight ratio adds EM bacterium stock solution and the fish flour of 1% of 1%, then by weight adding 40
～the water of 50%, after mixing, sealing and fermenting 4～5 days, make fly larvae culture matrix；
Step 3, fly larvae culture matrix is placed in cement conversion pool；
Step 4, on fly larvae culture matrix in 5000～10000/kg ratio access fly blow, be placed in 25～30 DEG C,
Cultivate under conditions of humidity 60～75%, after 4～5 days, the fly larvae entirety of fly larvae culture matrix and inside thereof dried, grind
Broken, make high protein edible bacterium culture medium substrate；
Step 5, according to edible fungus culturing needs, by high protein edible bacterium culture medium substrate and wood sawdust, cotton seed hulls, bran
Skin, sucrose, Gypsum Fibrosum powder and water etc. are combined by different proportion, make high protein edible bacterium culture medium.
Edible fungi residue described in step 1 is the culture medium residue of pocket type cultivation edible fungi.
Medicated beer distiller grains described in step 2 include yellow beer and the distiller grains brewageing generation of black beer.
Cement conversion pool specification described in step 3 is 120cm × 60cm × 20cm.
Fly blow described in step 4 includes housefly, lucilia sericata and Sarcophga fuscicauda fly blow；Described Eggs of Musca Domestica Vicina is 5000-
10000/kg；Described Sarcophga fuscicauda ovum or lucilia sericata ovum are 4000-8000 grain/kg.
Edible fungi described in step 5 includes pocket type cultivation edible fungi, such as Pleurotus ostreatus, Flammulina velutiper (Fr.) Sing, Grifola frondosa, Pleurotus eryngii, Bai Ling
Mushroom, Auricularia polytricha (Mout) Sacc., Hericium erinaceus (Bull. Ex Fr.) Pers., Lentinus Edodes, Cordyceps militaris (L.) Link.；Also include the edible fungi that earthing formula is cultivated, such as Caulis Bambusae In Taeniam and Coprinus comatus.
Fly larvae is a kind of preferably bioconversion material, and its growth rapidly, is suitable for all feeds and environment, and conduct
Feed protein additive is of high nutritive value, and containing thick protein 53.26-62%, crude fat 13.09%, various amino acid/11s 7 kinds, is
A kind of high protein is biological.
By wood sawdust used with classical culture protocols again for the high protein edible bacterium culture medium substrate containing fly larvae, cotton
Seed shell, wheat bran, corn cob, sucrose, Gypsum Fibrosum powder, water etc. are combined by different proportion, make high protein edible bacterium culture medium.
The method utilizing fly larvae that edible fungi residue changes into culture medium of edible fungus that the present invention provides solves edible fungi
Dreg cannot recycle problem, and the high protein edible bacterium culture medium of preparation reduces the toxigenic capacity of edible fungi, improves edible
The weight of bacterium and size, existing significant ecological benefits, also there is significant economic benefit.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
After step 1, Pleurotus eryngii dreg shine dry grinding, with 20 eye mesh screen screenings；
Step 2, will screening after Pleurotus eryngii dreg powder 60kg, dried poultrymanure 20kg, dry beer distiller grains 20kg mixing, then press
Add EM bacterium stock solution and the fish flour of 1kg of 1kg according to weight ratio, then by weight adding the water of 45kg, after mixing, pile up and cover
Polypropylence Sheet seals, and ferments 5 days, makes fly larvae culture matrix；
Step 3, take 10kg fly larvae culture matrix and be placed in the cement conversion pool of 120cm × 60cm × 20cm；
Step 4, on fly larvae culture matrix, access 80,000 Eggs of Musca Domestica Vicinas, after 4 days, by fly larvae culture matrix and inside thereof
Fly larvae entirety is dried, is ground, and makes high protein edible bacterium culture medium substrate；
Step 5, by high protein edible bacterium culture medium matter substrate, cotton seed hull, wood sawdust, sucrose, Gypsum Fibrosum powder according to 40:30:
The ratio of 28:1:1 makes high protein edible bacterium culture medium.
Use traditional method make control medium: by cotton seed hull, weed tree sawdust, corn cob, Gypsum Fibrosum powder, sucrose according to
The ratio of 50:24:24:1:1 makes tradition culture medium of edible fungus.
Every bag of 400g of high protein edible bacterium culture medium, inoculates Hericium erinaceus (Bull. Ex Fr.) Pers., if 3 culture bag are as repetition.
Tradition culture medium of edible fungus every bag 400g, inoculates Hericium erinaceus (Bull. Ex Fr.) Pers., if 3 culture bag are as repetition.
Synopsis after having cultivated is as shown in table 1.
The impact that Hericium erinaceus (Bull. Ex Fr.) Pers. is grown by table 1 high protein edible bacterium culture medium and control medium
| Culture medium classification | Mushroom fresh weight (g) | Bacteria cover diameter (cm) |
| High protein edible bacterium culture medium | 246.3±6.11a | 7.5±0.4a |
| Control medium | 212.7±10.5b | 6.2±0.6b |
Same column of figure, different letters represent significant difference (p < 0.05).
The result of table 1 shows, the high protein edible bacterium culture medium that the present invention provides is remarkably improved pocket type and cultivates Hericium erinaceus (Bull. Ex Fr.) Pers.
Weight and size, compared with control medium, mushroom fresh weight improve 15%, cap size improve 20%.
Embodiment 2
After step 1, needle mushroom dreg shine dry grinding, with 20 eye mesh screen screenings；
Step 2, will screening after golden mushroom ground-slag 50kg, dried poultrymanure 30kg, dry beer distiller grains 20kg mixing, then press
Add EM bacterium stock solution and the fish flour of 1kg of 1kg according to weight ratio, then by weight adding the water of 42kg, after mixing, pile up and cover
Polypropylence Sheet seals, and ferments 4 days, makes fly larvae culture matrix；
Step 3, take 10kg fly larvae culture matrix and be placed in the cement conversion pool of 120cm × 60cm × 20cm；
Step 4, in fly larvae culture medium, access 40,000 Sarcophga fuscicauda ovum, after 4 days, by fly larvae culture matrix and interior
The fly larvae entirety in portion is dried, is ground, and makes high protein edible bacterium culture medium substrate；
Step 5, by high protein edible bacterium culture medium matter substrate, cotton seed hull, wood sawdust, sucrose, Gypsum Fibrosum powder according to 45:38:
The ratio of 25:2:1 makes high protein edible bacterium culture medium.
Use traditional method make control medium: by cotton seed hull, weed tree sawdust, corn cob, Gypsum Fibrosum powder, sucrose according to
The ratio of 60:30:30:2:1 makes tradition culture medium of edible fungus.
Every bag of 500g of high protein edible bacterium culture medium, inoculates Pleurotus ostreatus, if 3 culture bag are as repetition.
Tradition culture medium of edible fungus every bag 500g, inoculates Pleurotus ostreatus, if 3 culture bag are as repetition.
Synopsis after having cultivated is as shown in table 2.
Table 2 high protein edible bacterium culture medium and the control medium impact on mushroom growth
| Culture medium classification | Mushroom fresh weight (g) | Bacteria cover diameter (cm) |
| High protein edible bacterium culture medium | 300±10a | 22±1a |
| Control medium | 250±12b | 18±1.2b |
Same column of figure, different letters represent significant difference (p < 0.05).
The result of table 2 shows, the high protein edible bacterium culture medium that the present invention provides is remarkably improved pocket type and cultivates Pleurotus ostreatus
Weight and size, compared with control medium, mushroom fresh weight improves 20%, and cap size improves 22%.
Embodiment 3
After step 1, Pleurotus ostreatus dreg shine dry grinding, with 20 eye mesh screen screenings；
Step 2, will screening after Pleurotus ostreatus dreg powder 40kg, dried poultrymanure 40kg, dry beer distiller grains 20kg mixing, then according to
Weight ratio adds EM bacterium stock solution and the fish flour of 1kg of 1kg, then by weight adding the water of 50kg, after mixing, piles up and cover and mould
Material cloth seals, and ferments 5 days, makes fly larvae culture matrix；
Step 3, take 10kg fly larvae culture matrix and be placed in the cement conversion pool of 120cm × 60cm × 20cm；
Step 4, in fly larvae culture medium access 70,000 lucilia sericata ovum, after 5 days, by fly larvae culture matrix and inside thereof
Fly larvae entirety dry, grind, make high protein edible bacterium culture medium substrate；
Step 5, by high protein edible bacterium culture medium matter substrate, cotton seed hull, wheat bran, Gypsum Fibrosum powder according to the ratio of 50:20:12:2
Example makes high protein edible bacterium culture medium.
Use the control medium that traditional method makes: by cotton seed hull, corn cob, carbamide, Gypsum Fibrosum powder according to 90:8:0.5:
The ratio of 1.5 makes tradition culture medium of edible fungus.
High protein edible bacterium culture medium earthing thickness 30cm, inoculates Coprinus comatus, if 3 hot houses are as repetition.
Tradition culture medium of edible fungus earthing thickness 30cm, inoculates Coprinus comatus, if 3 hot houses are as repetition.
Synopsis after having cultivated is as shown in table 3.
The impact that Coprinus comatus is grown by table 3 high protein edible bacterium culture medium and control medium
| Culture medium classification | Stem height (cm) | Bacteria cover diameter (cm) |
| High protein edible bacterium culture medium | 18±2a | 5±1a |
| Control medium | 14±2b | 4±0.5b |
Same column of figure, different letters represent significant difference (p < 0.05).
The result of table 3 shows, the high protein edible bacterium culture medium that the present invention provides is remarkably improved earthing formula cultivation drumsticks
The stem height of mushroom and cap size, compared with control medium, stem height improves 14%, and cap size improves 25%.
Claims (5)
1. utilize the method that edible fungi residue is changed into culture medium of edible fungus by fly larvae, it is characterised in that comprise the steps of
After step 1, edible fungi residue shine dry grinding, with 20 eye mesh screen screenings；
Step 2, by the edible fungi ground-slag after screening, dried poultrymanure and dry beer distiller grains with the ratio of weight ratio 4～6:2～4:2
Mixing, then according to above raw material gross weight than add 1% EM bacterium stock solution and the fish flour of 1%, then by weight addition 40～
The water of 50%, after mixing, sealing and fermenting 4～5 days, make fly larvae culture matrix；
Step 3, fly larvae culture matrix is placed in cement conversion pool；
Step 4, on fly larvae culture matrix in 4000～10000/kg ratio access fly blow, be placed in 25～30 DEG C, humidity
Cultivate under conditions of 60～75%, after 4～5 days, the fly larvae entirety of fly larvae culture matrix and inside thereof dried, ground,
Make high protein edible bacterium culture medium substrate；
Step 5, according to edible fungus culturing needs, by high protein edible bacterium culture medium substrate and wood sawdust, cotton seed hulls, wheat bran, sugarcane
Sugar, Gypsum Fibrosum powder and water etc. are combined by different proportion, make high protein edible bacterium culture medium.
The method utilizing fly larvae that edible fungi residue changes into culture medium of edible fungus the most according to claim 1, its feature
Being, the fly blow described in step 4 includes housefly, lucilia sericata and Sarcophga fuscicauda fly blow；Described Eggs of Musca Domestica Vicina inoculum concentration is 5000-
10000/kg；Described Sarcophga fuscicauda ovum or lucilia sericata ovum inoculum concentration are 4000-8000 grain/kg.
The method utilizing fly larvae that edible fungi residue changes into culture medium of edible fungus the most according to claim 1 and 2, it is special
Levying and be, the edible fungi residue described in step 1 is the culture medium residue of pocket type cultivation edible fungi.
The method utilizing fly larvae that edible fungi residue changes into culture medium of edible fungus the most according to claim 1 and 2, it is special
Levying and be, the medicated beer distiller grains described in step 2 include yellow beer and the distiller grains brewageing generation of black beer.
The method utilizing fly larvae that edible fungi residue changes into culture medium of edible fungus the most according to claim 1 and 2, it is special
Levying and be, the edible fungi described in step 5 includes pocket type cultivation edible fungi；Also include the edible fungi that earthing formula is cultivated.
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|---|---|---|---|
| CN201610538301.9A CN106167424A (en) | 2016-07-08 | 2016-07-08 | Utilize the method that edible fungi residue is changed into culture medium of edible fungus by fly larvae |
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| CN201610538301.9A CN106167424A (en) | 2016-07-08 | 2016-07-08 | Utilize the method that edible fungi residue is changed into culture medium of edible fungus by fly larvae |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805201A (en) * | 2011-06-01 | 2012-12-05 | 刘成军 | Method for making magnetic fly maggot feed |
| CN103583901A (en) * | 2013-11-07 | 2014-02-19 | 周辛平 | Fly and maggot cultivation method and high protein feed adopting same |
| CN104785509A (en) * | 2015-04-17 | 2015-07-22 | 泰山医学院 | Method for bioconversion of edible mushroom dregs with housefly larvae |
-
2016
- 2016-07-08 CN CN201610538301.9A patent/CN106167424A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805201A (en) * | 2011-06-01 | 2012-12-05 | 刘成军 | Method for making magnetic fly maggot feed |
| CN103583901A (en) * | 2013-11-07 | 2014-02-19 | 周辛平 | Fly and maggot cultivation method and high protein feed adopting same |
| CN104785509A (en) * | 2015-04-17 | 2015-07-22 | 泰山医学院 | Method for bioconversion of edible mushroom dregs with housefly larvae |
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