CN106164260A - 假丝酵母及其羰基还原酶的产生及应用 - Google Patents

假丝酵母及其羰基还原酶的产生及应用 Download PDF

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CN106164260A
CN106164260A CN201580002695.XA CN201580002695A CN106164260A CN 106164260 A CN106164260 A CN 106164260A CN 201580002695 A CN201580002695 A CN 201580002695A CN 106164260 A CN106164260 A CN 106164260A
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caprylate
halo
carbonyl
carbonyl reductase
hydroxyl
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CN106164260B (zh
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许建和
张玉军
郑高伟
潘江
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East China University of Science and Technology
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Abstract

本发明公开了近平滑假丝酵母CGMCC 9630，该菌株表达的羰基还原酶及其编码基因和氨基酸序列，含有该基因序列的重组表达载体和重组表达转化体，以及利用所述近平滑假丝酵母整细胞、其羰基还原酶或相应的重组转化体作为催化剂用于催化潜手性羰基化合物的不对称还原，特别是6‑羰基‑8‑卤代辛酸酯还原制备(R)‑α‑硫辛酸合成前体(R)‑6‑羟基‑8‑卤代辛酸酯。与不对称还原制备(R)‑6‑羟基‑8‑卤代辛酸酯的其他方法相比，本发明具有底物浓度高、反应条件温和、环境友好、产率高、产品光学纯度高等优势，因此在(R)‑α‑硫辛酸的工业化生产中具有很好的应用前景。

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PCT国内申请，说明书已公开。

Claims (1)

  1. PCT国内申请，权利要求书已公开。
CN201580002695.XA 2015-03-04 2015-03-04 一种假丝酵母羰基还原酶及用于制备(r)-6-羟基-8-氯辛酸酯的方法 Active CN106164260B (zh)

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CN110004119A (zh) * 2019-04-18 2019-07-12 华东理工大学 ε-酮酯还原酶突变体及其催化合成(R)-α-硫辛酸前体的应用
CN110408638A (zh) * 2019-06-19 2019-11-05 南京趣酶生物科技有限公司 硫辛酸手性醇中间体的制备方法及其新酶催化剂的制备方法
CN113174377A (zh) * 2021-04-28 2021-07-27 华东理工大学 羰基还原酶、突变体及其在制备地尔硫卓中间体中的应用
CN117887679A (zh) * 2024-03-14 2024-04-16 山东金城生物药业有限公司 羰基还原酶突变体及其在制备(s)-玻色因中的应用

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CN115927224A (zh) * 2021-08-05 2023-04-07 上海医药工业研究院 一种羰基还原酶突变体及其应用
CN117247911B (zh) * 2023-08-22 2024-06-07 武汉爱博泰克生物科技有限公司 大肠杆菌dna连接酶突变体及其在毕赤酵母中表达纯化方法
CN117230031B (zh) * 2023-11-03 2024-01-26 山东理工大学 羰基还原酶突变体及其应用

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110004119A (zh) * 2019-04-18 2019-07-12 华东理工大学 ε-酮酯还原酶突变体及其催化合成(R)-α-硫辛酸前体的应用
CN110004119B (zh) * 2019-04-18 2020-10-20 华东理工大学 ε-酮酯还原酶突变体及其催化合成(R)-α-硫辛酸前体的应用
CN110408638A (zh) * 2019-06-19 2019-11-05 南京趣酶生物科技有限公司 硫辛酸手性醇中间体的制备方法及其新酶催化剂的制备方法
CN113174377A (zh) * 2021-04-28 2021-07-27 华东理工大学 羰基还原酶、突变体及其在制备地尔硫卓中间体中的应用
CN117887679A (zh) * 2024-03-14 2024-04-16 山东金城生物药业有限公司 羰基还原酶突变体及其在制备(s)-玻色因中的应用
CN117887679B (zh) * 2024-03-14 2024-06-11 山东金城生物药业有限公司 羰基还原酶突变体及其在制备(s)-玻色因中的应用

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