CN106136085B - A kind of natural semen sojae atricolor medicinal extract and preparation method thereof - Google Patents
A kind of natural semen sojae atricolor medicinal extract and preparation method thereof Download PDFInfo
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- CN106136085B CN106136085B CN201610474075.2A CN201610474075A CN106136085B CN 106136085 B CN106136085 B CN 106136085B CN 201610474075 A CN201610474075 A CN 201610474075A CN 106136085 B CN106136085 B CN 106136085B
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- semen sojae
- sojae atricolor
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- 210000000582 semen Anatomy 0.000 title claims abstract description 55
- 239000000284 extract Substances 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 238000000605 extraction Methods 0.000 title abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 4
- PYMYPHUHKUWMLA-VPENINKCSA-N aldehydo-D-xylose Chemical compound OC[C@@H](O)[C@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-VPENINKCSA-N 0.000 claims abstract description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 4
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 239000006228 supernatant Substances 0.000 claims description 12
- 108091005804 Peptidases Proteins 0.000 claims description 9
- 239000004365 Protease Substances 0.000 claims description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 9
- 235000019419 proteases Nutrition 0.000 claims description 9
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 8
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 6
- 229940088598 enzyme Drugs 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 239000013049 sediment Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 102000004139 alpha-Amylases Human genes 0.000 claims description 3
- 108090000637 alpha-Amylases Proteins 0.000 claims description 3
- 229940024171 alpha-amylase Drugs 0.000 claims description 3
- 239000000084 colloidal system Substances 0.000 claims description 3
- 239000000796 flavoring agent Substances 0.000 claims description 3
- 235000019634 flavors Nutrition 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 230000004044 response Effects 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 238000010792 warming Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 2
- 238000002386 leaching Methods 0.000 claims 1
- 208000020442 loss of weight Diseases 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 13
- 235000010627 Phaseolus vulgaris Nutrition 0.000 abstract description 10
- 244000046052 Phaseolus vulgaris Species 0.000 abstract description 10
- 230000000996 additive effect Effects 0.000 abstract description 7
- 239000000126 substance Substances 0.000 abstract description 7
- 239000000654 additive Substances 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 3
- 239000003795 chemical substances by application Substances 0.000 abstract description 2
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- 239000003205 fragrance Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 206010013496 Disturbance in attention Diseases 0.000 description 2
- 230000036528 appetite Effects 0.000 description 2
- 235000019789 appetite Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 241001107116 Castanospermum australe Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000021279 black bean Nutrition 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 239000013538 functional additive Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 1
- 150000002515 isoflavone derivatives Chemical class 0.000 description 1
- 235000008696 isoflavones Nutrition 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000014860 sensory perception of taste Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of natural semen sojae atricolor medicinal extract and preparation method thereof, and the semen sojae atricolor medicinal extract is extracted and is prepared from following raw material components and its corresponding parts by weight: semen sojae atricolor, and 100 parts；Reduced sugar, 2 parts；Pure water, 1300 parts；The reduced sugar is made by D-Glucose and D- xylose by 2:1 weight ratio, passes sequentially through step-up temperature extraction, and biological enzymolysis extracts, and the preparation step of natural semen sojae atricolor medicinal extract is made by three steps.A kind of natural semen sojae atricolor medicinal extract provided by the invention, the mouthfeel of food after addition is natural, aroma of pure, there is no the uncoordinated feeling of chemical addition agent smell bring, the a variety of needs for containing more benefit materials in it, meeting human body, additive effect synthesizes beans essence additive better than traditional chemical, increases the trophism of food, and safe to the human body without side-effects, meet pursuit of the people for natural food, is the pure natural substitute of existing chemically synthesized beans essence.
Description
Technical field
The present invention relates to a kind of field of food producing technology, and in particular to a kind of pure natural of pure natural, fragrance
Semen sojae atricolor medicinal extract and preparation method thereof.
Background technique
The nutriments such as semen sojae atricolor protein rich in, fat, amino acid, and it is living rich in isoflavones, polysaccharide, pigment etc.
Property substance, given activity substance is extracted from the casting skin of semen sojae atricolor and is used as functional additive, has there is a product appearance, and utilization is black
Beans extract manufactures and replaces traditional beans essence additive (including soya-bean milk essence, beans essence, soya bean essence, fermented soya bean essence etc.)
Technology do not find temporarily；For the fragrance of beans essence with the fragrance of natural bean product there are no small difference, natural sense is not strong simultaneously,
The people having a sharp sense of taste can feel flatly, seriously affect the appetite and desire to buy of customer；In addition with the development of society, people
The health of food is increasingly paid attention to, pure natural addictive replaces chemical synthesis additive and essence, has become food service industry
Therefore how the trend of development is developed in a kind of skin and pulp from semen sojae atricolor and extracts and utilize its active constituent, satisfaction food is made
The requirement of product addition, mouthfeel is natural, aroma of pure, benefit materials are more, additive effect better than traditional chemical synthesizes beans essence
Additive, pure natural Semen sojae atricolor extract or semen sojae atricolor medicinal extract are the targets that food manufacture field technical staff makes great efforts.
Summary of the invention
In view of the deficiencies of the prior art, the present invention intends to provide a kind of natural semen sojae atricolor medicinal extract and its production side
Method.
The first object of the present invention is to provide a kind of natural semen sojae atricolor medicinal extract, above-mentioned semen sojae atricolor medicinal extract from following raw material components and
It extracts and is prepared in its corresponding parts by weight: semen sojae atricolor, 100 parts；Reduced sugar, 2 parts；Pure water, 1300 parts；Above-mentioned reduced sugar by
D-Glucose and D- xylose are made by 2:1 weight ratio.
The second object of the present invention is to provide the preparation method of above-mentioned natural semen sojae atricolor medicinal extract, and steps are as follows for the above method:
Step (1): step-up temperature is extracted, and the semen sojae atricolor of the impurity such as selected damaged out, mildew is cleaned after weighing with tap water
Completely；By semen sojae atricolor: after pure water=1:8 weight ratio adds pure water to impregnate, 4 ~ 5h is cooked under the conditions of being heated to 95 ~ 100 DEG C, so
Heating continues to cook under air-proof condition afterwards；After being cooled to room temperature, under the conditions of 5000rpm ~ 8000rpm be centrifuged 10 ~
15min collects supernatant respectively and sediment is stand-by.
Step (2): biological enzymolysis extracts, and obtained above-mentioned sediment will be extracted in step (1), 5 times of semen sojae atricolor weight are added
Pure water, broken with 200 mesh colloid mills after mixing evenly, 0.2% alpha-amylase for then adding semen sojae atricolor weight carries out first
Secondary enzymatic hydrolysis；It is cooled to 50 DEG C later, then adds 0.5% compound protease progress, second of enzymatic hydrolysis of semen sojae atricolor weight, is finally warming up to
95 ~ 100 DEG C, inactive enzyme under the conditions of heat preservation 15min, are centrifuged 10 ~ 15min under the conditions of 5000rpm ~ 8000rpm, and separation removes slag,
It is stand-by to obtain supernatant.
Step (3): the preparation of natural semen sojae atricolor medicinal extract, by the supernatant that step (1) obtains and the supernatant that step (2) obtains
Mixing, adds reduced sugar in proportion, 1.5 ~ 2h of thermal response under conditions of 121 DEG C of temperature, pH6, through vacuum concentration loss of weight 30 ~
Up to natural semen sojae atricolor medicinal extract after 40%.
Above-mentioned preparation method of the invention is further arranged to: above-mentioned compound protease by 2:1 weight ratio neutral egg
White enzyme and flavor protease form.
Above-mentioned preparation method of the invention is further arranged to: above-mentioned soaking temperature be 4 ~ 10 DEG C, soaking time be 8 ~
10h；Temperature is 115 ~ 121 DEG C under above-mentioned air-proof condition, and above-mentioned continuation brew time is 2 ~ 3h.
Above-mentioned preparation method of the invention is further arranged to: the condition of above-mentioned first time enzymatic hydrolysis are as follows: 90 ~ 95 DEG C,
PH5.5, first time enzymolysis time are 30min.
Above-mentioned preparation method of the invention is further arranged to: the condition of above-mentioned second of enzymatic hydrolysis are as follows: 50 ~ 55 DEG C,
PH5.5, second of enzymolysis time are 6 ~ 8h.
By using above-mentioned technical proposal, the present invention is had an advantage that
1. by from extracting and be made the medicinal extract being added in food in semen sojae atricolor, the mouthfeel of food is natural, aroma of pure, does not have
There is the uncoordinated feeling of chemical addition agent smell bring, the class for improving food and naturally sense.
2. the semen sojae atricolor medicinal extract of pure natural extraction, the interior a variety of needs for containing more benefit materials, meeting human body, addition
Effect synthesizes beans essence additive better than traditional chemical, increases the trophism of food.
3. pure natural semen sojae atricolor medicinal extract, and it is safe to the human body without side-effects, meet pursuit of the people for natural food,
The appetite and desire to purchase that people can be stimulated facilitate the marketing and application of pure natural semen sojae atricolor medicinal extract.
4. pure natural semen sojae atricolor medicinal extract, it is existing chemically synthesized beans essence that effect, which is better than traditional beans essence,
Pure natural substitute.
Detailed description of the invention
Fig. 1 is process flow chart of the invention.
Specific embodiment
Embodiment 1
A kind of natural semen sojae atricolor medicinal extract, semen sojae atricolor medicinal extract extracted from following raw material components and its corresponding parts by weight and prepare and
At: semen sojae atricolor, 100 parts；Reduced sugar, 2 parts；Pure water, 1300 parts；Reduced sugar presses 2:1 weight ratio by D-Glucose and D- xylose
It is made into.
Embodiment 2
In conjunction with the embodiments 1, a kind of preparation method of natural semen sojae atricolor medicinal extract, steps are as follows for the above method:
Step (1): step-up temperature is extracted, and the semen sojae atricolor of the impurity such as selected damaged out, mildew is cleaned after weighing with tap water
Completely；By semen sojae atricolor: after pure water=1:8 weight ratio adds pure water to impregnate, 4 ~ 5h is cooked under the conditions of being heated to 95 ~ 100 DEG C, so
Heating continues to cook under air-proof condition afterwards；After being cooled to room temperature, under the conditions of 5000rpm ~ 8000rpm be centrifuged 10 ~
15min collects supernatant respectively and sediment is stand-by.
Step (2): biological enzymolysis extracts, and obtained sediment will be extracted in step (1), the pure of 5 times of semen sojae atricolor weight is added
Water, broken with 200 mesh colloid mills after mixing evenly, 0.2% alpha-amylase for then adding semen sojae atricolor weight carries out first time enzyme
Solution；It is cooled to 50 DEG C later, then adds 0.5% compound protease of semen sojae atricolor weight and carries out second and digest, finally it is warming up to 95 ~
100 DEG C, inactive enzyme under the conditions of heat preservation 15min, are centrifuged 10 ~ 15min, separation removes slag, obtain under the conditions of 5000rpm ~ 8000rpm
Supernatant is stand-by.
Step (3): the preparation of natural semen sojae atricolor medicinal extract, by the supernatant that step (1) obtains and the supernatant that step (2) obtains
Mixing, adds reduced sugar in proportion, 1.5 ~ 2h of thermal response under conditions of 121 DEG C of temperature, pH6, through vacuum concentration loss of weight 30 ~
Up to natural semen sojae atricolor medicinal extract after 40%.
Compound protease is made of the neutral proteinase of 2:1 weight ratio with flavor protease.
Soaking temperature is 4 ~ 10 DEG C, and soaking time is 8 ~ 10h；Temperature is 115 ~ 121 DEG C under air-proof condition, when continuing to cook
Between be 2 ~ 3h.
The condition of enzymatic hydrolysis for the first time are as follows: 90 ~ 95 DEG C, pH5.5, first time enzymolysis time are 30min.
The condition of second of enzymatic hydrolysis are as follows: 50 ~ 55 DEG C, pH5.5, second of enzymolysis time are 6 ~ 8h.
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-mentioned implementation
Example, all technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
Those of ordinary skill for, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (5)
1. a kind of preparation method of natural semen sojae atricolor medicinal extract, which is characterized in that the semen sojae atricolor medicinal extract is from following raw material components and its phase
Ying Chong
It extracts and is prepared in amount part:
Semen sojae atricolor, 100 parts；
Reduced sugar, 2 parts；
Pure water, 1300 parts；
The reduced sugar is made by D-Glucose and D- xylose by 2:1 weight ratio；
The method comprises the following steps:
Step (1) step-up temperature is extracted: the semen sojae atricolor of the impurity such as selected damaged out, mildew is cleaned up after weighing with tap water；
By semen sojae atricolor: after pure water=1:8 weight ratio adds pure water to impregnate, 4 ~ 5h is cooked under the conditions of being heated to 95 ~ 100 DEG C, then close
Heating continues to cook under the conditions of envelope；After being cooled to room temperature, 10 ~ 15min is centrifuged under the conditions of 5000rpm ~ 8000rpm, respectively
It collects supernatant and sediment is stand-by；
Step (2) biological enzymolysis extracts: will extract the obtained sediment in step (1) and the pure of 5 times of semen sojae atricolor weight is added
Water, broken with 200 mesh colloid mills after mixing evenly, 0.2% alpha-amylase for then adding semen sojae atricolor weight carries out first time enzyme
Solution；It is cooled to 50 DEG C later, then adds 0.5% compound protease of semen sojae atricolor weight and carries out second and digest, finally it is warming up to 95 ~
100 DEG C, inactive enzyme under the conditions of heat preservation 15min, are centrifuged 10 ~ 15min, separation removes slag, obtain under the conditions of 5000rpm ~ 8000rpm
Supernatant is stand-by；
The preparation of the natural semen sojae atricolor medicinal extract of step (3): the supernatant that step (1) obtains and the supernatant that step (2) obtains are mixed,
Reduced sugar is added in proportion, 1.5 ~ 2h of thermal response under conditions of 121 DEG C of temperature, pH6, after being concentrated in vacuo loss of weight 30 ~ 40% i.e.
Obtain natural semen sojae atricolor medicinal extract.
2. the preparation method of natural semen sojae atricolor medicinal extract described in claim 1, which is characterized in that the compound protease is by 2:1 weight
The neutral proteinase and flavor protease of ratio form.
3. the preparation method of natural semen sojae atricolor medicinal extract described in claim 1, which is characterized in that the soaking temperature is 4 ~ 10 DEG C, leaching
The bubble time is 8 ~ 10h；Temperature is 115 ~ 121 DEG C under the air-proof condition, and the continuation brew time is 2 ~ 3h.
4. the preparation method of natural semen sojae atricolor medicinal extract described in claim 1, which is characterized in that the condition of the first time enzymatic hydrolysis are as follows:
90 ~ 95 DEG C, pH5.5, first time enzymolysis time are 30min.
5. the preparation method of natural semen sojae atricolor medicinal extract described in claim 1, which is characterized in that the condition of second of enzymatic hydrolysis are as follows:
50 ~ 55 DEG C, pH5.5, second of enzymolysis time are 6 ~ 8h.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610474075.2A CN106136085B (en) | 2016-06-27 | 2016-06-27 | A kind of natural semen sojae atricolor medicinal extract and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610474075.2A CN106136085B (en) | 2016-06-27 | 2016-06-27 | A kind of natural semen sojae atricolor medicinal extract and preparation method thereof |
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| Publication Number | Publication Date |
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| CN106136085A CN106136085A (en) | 2016-11-23 |
| CN106136085B true CN106136085B (en) | 2019-10-18 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111134326B (en) * | 2018-11-02 | 2023-04-07 | 内蒙古伊利实业集团股份有限公司 | Black bean extracting solution and preparation method thereof |
| CN111213840A (en) * | 2018-11-26 | 2020-06-02 | 内蒙古伊利实业集团股份有限公司 | Black bean concentrated solution, solid black bean product and production and processing methods thereof |
| CN111772108A (en) * | 2019-04-03 | 2020-10-16 | 内蒙古伊利实业集团股份有限公司 | Natural black bean extract and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101457248A (en) * | 2008-11-12 | 2009-06-17 | 广州华宝香精香料有限公司 | Method for preparing maillard flavor peptides by using soja bean separation protein |
| CN103892241A (en) * | 2014-04-08 | 2014-07-02 | 浙江省农业科学院 | Natural beef flavor spice and production method thereof |
| CN105309913A (en) * | 2014-07-18 | 2016-02-10 | 广州御膳记生物科技有限公司 | Production technology for novel natural extractive condiment |
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2016
- 2016-06-27 CN CN201610474075.2A patent/CN106136085B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101457248A (en) * | 2008-11-12 | 2009-06-17 | 广州华宝香精香料有限公司 | Method for preparing maillard flavor peptides by using soja bean separation protein |
| CN103892241A (en) * | 2014-04-08 | 2014-07-02 | 浙江省农业科学院 | Natural beef flavor spice and production method thereof |
| CN105309913A (en) * | 2014-07-18 | 2016-02-10 | 广州御膳记生物科技有限公司 | Production technology for novel natural extractive condiment |
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