CN106134994A - A kind of artificial cultivation method of radix ranunculi ternati - Google Patents
A kind of artificial cultivation method of radix ranunculi ternati Download PDFInfo
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- CN106134994A CN106134994A CN201610493896.0A CN201610493896A CN106134994A CN 106134994 A CN106134994 A CN 106134994A CN 201610493896 A CN201610493896 A CN 201610493896A CN 106134994 A CN106134994 A CN 106134994A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
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- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention discloses the artificial cultivation method of a kind of Chinese herbal medicine radix ranunculi ternati, the method of radix ranunculi ternati holder explant Fiber differentiation is utilized first in terms of Chinese herbal medicine artificial cultivation, induction is cultivated and is obtained radix ranunculi ternati seedling, obtain the radix ranunculi ternati block root that can use as Chinese medicine after domestication, hardening, transplanting breeding, also obtain excellent radix ranunculi ternati germ plasm resource.The present invention cultivates, through callus differentiation, the radix ranunculi ternati seedling obtaining, both the region having overcome artificial transplanting limits and cost disadvantage, obtain gene atypical of germ plasm resource when overcoming again by seminal propagation, have in the application prospect promoting radix ranunculi ternati artificial cultivation field.
Description
Technical field
The present invention relates to the artificial cultivation method of a kind of radix ranunculi ternati, be specifically related to a kind of based on the cultivation of radix ranunculi ternati vitro anther
Artificial cultivation method.
Background technology
Radix ranunculi ternati is Ranunculaceae herbaceos perennial Seynuns Kugelann, is used as medicine with block root.Mainly originate in Jiangsu, Zhejiang, Anhui,
The areas such as Jiangxi, Henan, Hubei, Guizhou, Guangxi.Micro-sweet, the property of radix ranunculi ternati taste is put down, and enters lung, liver two warp, has dissipating bind, detumescence, clearly
Heat, the function of removing toxic substances.Cure mainly pulmonary tuberculosis, scrofula, sphagitis, hemorrhoid etc. on neck, also can treat kinds cancer, lymthoma etc., anti-
Cancer effect is notable, and cure rate is high, is antitumor in Chinese medicine, removing toxic substances, the good medicinal material of one of dissipating bind.
Owing to radix ranunculi ternati is wild, excavate conveniently, there is wide curative effect, the huge market demand, and price comparison high, draw
Send out producing region peasant actively to excavate, make radix ranunculi ternati wild resource day by day reduce, become Chinese medicine in short supply at present, therefore, Planting
Training radix ranunculi ternati becomes a kind of inevitable trend.
The method of prior art artificial cultivation radix ranunculi ternati mainly artificial transplanting, block root propagation, seminal propagation.Due to wild
The low cost of radix ranunculi ternati and some areas are relatively conventional, and medicinal herb grower uses the method cultivation radix ranunculi ternati of artificial transplanting mostly, but should
Method is only applicable to the environment with a large amount of wild radix ranunculi ternati, is not suitable for most of area.Block root propagation is to utilize radix ranunculi ternati
The method of the other fritter root cuttage plantation of block root realize artificial propagation, the coefficient of block root propagation is low, it is impossible to meet extensive
The needs producing, and owing to the factors such as culture environment such as illumination, moisture, temperature, nutrition etc. and hormone concentration easily cause cat
There is asexual variation in pawl grass seeds seedling.Ranunculaceae plant has very big variability, and radix ranunculi ternati is also resulted in by cross pollination
Its gene mixes, and the seed being obtained by the method does not reaches expected medicinal effects after continuing to cultivate, and easily causes palm fibre in radix ranunculi ternati
The disappearance of the active ingredients such as ethyl gallate, myristic acid, palmitic acid, cupreol, stigmasterol, campesterol.
Content of the invention
For overcoming the defect of prior art, it is an object of the invention to provide the artificial cultivation method of a kind of radix ranunculi ternati, specifically
Step is as follows:
(1) holder explant pretreatment:
Choose the bud of radix ranunculi ternati, after refrigerating 3～5 days in 4 DEG C of environment, bud is taken out clear-cutting forestland to room temperature, stripping
Removing bract and little Hua, rinsing with pure water, first sterilize in the mercuric chloride aqueous solution of 0.05% 5～8min, then with 70% alcohol
Sterilization 20～40s, uses aseptic water washing 3 times afterwards, i.e. obtains radix ranunculi ternati holder explant.The percentage that above is referred to is body
Long-pending percentage.
(2) explant inoculation and Fiber differentiation:
In the first culture medium after radix ranunculi ternati holder explant is moved into sterilizing, temperature is set as 23 ± 1 DEG C, with indigo plant
Light modulation is light source, and intensity of illumination control is 400～800Lx, and photoperiod control is the dark 10h of light 14h/, and replacing in every ten days is once trained
Support base, until inducing callus.
The constituent of described first culture medium is: KNO3800～1300mg/L, 2-amino-5-carboxyl pentanamide 800
～1200mg/L, KH2PO4300～450mg/L, CaCl2·2H2O 320～350mg/L, MgSO4·7H2O 180～240mg/
L, EDTA 120～180mg/L, FeSO4·7H2O 30～50mg/L, MnSO4·4H2O 50～80mg/L, ZnSO4·7H2O
15～30mg/L, H3BO38～10mg/L, KI 5～10mg/L, Na2MoO4·2H2O 1～5mg/L, CoCl2·6H2O 0.2
～0.5mg/L, 4-iodobenzene fluoroacetic acid 0.15～0.20mg/L, inositol 30～80mg/L, glycine 0.5～1.0mg/L, vitamin
B1 0.5～0.75mg/L, vitamin B6 1.0～1.5mg/L, nicotinic acid 1.0～1.5mg/L, sucrose 30～50g/L, agar 10
～15g/L, kinetin KT 0.5～1.0mg/L, methyl α-naphthyl acetate NAA0.3～0.4mg/L, sorbierite 50～70g/L, biotin
0.15～0.20mg/L, puridoxine hydrochloride 0.5～1.0mg/L, thiamine hydrochloride 0.5～1.0mg/L, methyl-nitroso-urea 1.5
～2.0mg/L, indolebutyric acid 1.5～2.0mg/L, alanine 5～10mg/L, folic acid 5～10mg/L, Prohexadione calcium 1.5～
2.0mg/L, colchicin 1.5～2.0mg/L, protein hydrolysate 2.0～2.5g/L.
(3) Fiber differentiation is shifted:
The callus that step (2) obtains is transferred to the second culture medium, and temperature is 27 ± 1 DEG C, with fluorescent lamp as light source,
Intensity of illumination control is 1800～2500Lx, and photoperiod control is the dark 10h of light 14h/, after callus induction goes out tender shoots, by tender
Bud intactly cuts from radix ranunculi ternati holder explant, continues with identical CMC model to formation in transferring to the 3rd culture medium
Radix ranunculi ternati seedling.
The constituent of described second culture medium with the addition of indolebutyric acid on described first medium base of step (2)
0.5～2.0mg/L and 2,4 dichlorophenoxyacetic acid butyl ester 1.5～2.0mg/L.
The constituent of described 3rd culture medium with the addition of on described second medium base 6-benzyl aminopurine 1.5～
2.0mg/L。
(4) domestication of seedling and hardening:
By in radix ranunculi ternati seedling replanting to hardening matrix, and progressively transfer in external environment condition.
The constituent of described hardening matrix is: perlite, vermiculite, sawdust, pine bark, sphagna, flower mud, peat soil and
Fused calcium magnesium phosphorus potash fertilizer, mass ratio be 1:0.5～1.5:0.2～0.8:0.2～0.8:0.1～0.5:0.05～0.15:0.05～
0.15:0.005～0.015, preferred mass is than for 1:1:0.5:0.5:0.3:0.1:0.1:0.01.
(5) transplant:
Selecting soil layer hillside deep, well-drained or wasteland as field to be broadcast, thin after-applied base fertilizer is ploughed in leveling, in step
(4), after the seedling described in adapts to external environment condition normal growth, transplant to described field to be broadcast, line-spacing 10～15cm, spacing in the rows 5～8cm.
Described base fertilizer includes radix ranunculi ternati Special slow release fertilizer, and described radix ranunculi ternati Special slow release fertilizer forms and parts by weight are: urine
Element 100 parts, polysaccharide 50～100 parts, 20～25 parts of ammonium hydrogen carbonate, 12～14 parts of ammonium hydrogen carbonate, 3～6 parts of calcium nitrate, di(2-ethylhexyl)phosphate
Ammonium 50～60 parts, 12～14 parts of calcium magnesium phosphate, potassium sulfate 50～60 parts, zinc ammonium phosphate 3～5 parts, potassium silicate 4～7 parts, borax 1
～3 parts, manganese sulfate 1～3 part.
Wherein polysaccharide provides required biological carbon energy for radix ranunculi ternati growth, promotes the synthesis of sterol and acid.Excellent
Selection of land, the parts by weight of described polysaccharide are 65 parts.
(6) field management:
Carry out in time in growth period loosening the soil, water, the Routine Management such as weeding；Pest management aspect, for ensureing radix ranunculi ternati
Chinese medicine characteristic, agricultural chemicals is not applied to the insect pest that general harmfulness is little, to disease then to put prevention first, after disease occurs
Chinese medicine sterilizing liquid is used to carry out sterilization processing to radix ranunculi ternati.
(7) results and seed collecting:
After radix ranunculi ternati cultivation year and a day, digging out the block root of its under ground portion root, the seed of aerial part is adopted
Collection, completes artificial cultivation and the breeding of radix ranunculi ternati simultaneously, not only obtains economic benefit but also obtain stable radix ranunculi ternati germ plasm resource.
The present invention utilizes the method for Chinese herbal medicine radix ranunculi ternati holder explant Fiber differentiation, carries out artificial cultivation to radix ranunculi ternati,
This still belongs to the first time in terms of Chinese herbal medicine artificial cultivation.The artificial cultivation method of a kind of radix ranunculi ternati that the present invention relates to, uses ram's horn
The holder explant of grass obtains radix ranunculi ternati seedling through Fiber differentiation, shortens the callus divergaence time of callus, improves callus
The quantity of formation of tissue and efficiency.Cultivate, through callus differentiation, the radix ranunculi ternati obtaining, both overcome the region of artificial transplanting limit and become
This inferior position, obtains gene atypical of germ plasm resource when overcoming again by seminal propagation.The present invention has popularization cat
The application prospect of pawl strawman work cultivation field.
Detailed description of the invention
Below by specific embodiment, further technical scheme is specifically described.It should be understood that below
Embodiment be intended only as illustrating, and do not limit the scope of the invention, those skilled in the art is according to the present invention simultaneously
The obvious change made and modification are also contained within the scope of the invention.
Embodiment 1
A kind of radix ranunculi ternati in-vitro breeding method, specifically comprises the following steps that
(1) holder explant pretreatment:
Choose the bud of radix ranunculi ternati, after refrigerating 3 days in 4 DEG C of environment, by bud taking-up clear-cutting forestland to room temperature, peel off bud
Piece and little Hua, rinse with pure water, and first sterilize in the mercuric chloride aqueous solution of 0.05% 5min, then with 70% alcohol disinfecting 30s,
Use aseptic water washing 3 times afterwards, i.e. obtain radix ranunculi ternati holder explant；The percentage that above is referred to is percent by volume.
(2) explant inoculation and Fiber differentiation:
In the first culture medium after radix ranunculi ternati holder explant is moved into sterilizing, temperature is set as 23 ± 1 DEG C, with indigo plant
Light modulation is light source, and intensity of illumination control is 600Lx, and photoperiod control is the dark 10h of light 14h/, within every ten days, changes a subculture,
Until inducing callus.
The constituent of described first culture medium is: KNO3900mg/L, 2-amino-5-carboxyl pentanamide 1000mg/L,
KH2PO4360mg/L, CaCl2·2H2O 340mg/L, MgSO4·7H2O 190mg/L, EDTA 150mg/L, FeSO4·7H2O
40mg/L, MnSO4·4H2O 60mg/L, ZnSO4·7H2O 20mg/L, H3BO39mg/L, KI 8mg/L, Na2MoO4·2H2O
3mg/L, CoCl2·6H2O 0.3mg/L, 4-iodobenzene fluoroacetic acid 0.18mg/L, inositol 50mg/L, glycine 0.8mg/L, vitamin
B1 0.65mg/L, vitamin B6 1.3mg/L, nicotinic acid 1.1mg/L, sucrose 40g/L, agar 12g/L, kinetin KT 0.8mg/
L, methyl α-naphthyl acetate NAA0.35mg/L, sorbierite 60g/L, biotin 0.18mg/L, puridoxine hydrochloride 0.8mg/L, thiamine hydrochloride
0.8mg/L, methyl-nitroso-urea 1.6mg/L, indolebutyric acid 1.6mg/L, third ammonia 8mg/L, folic acid 8mg/L, Prohexadione calcium
1.7mg/L, colchicin 1.8mg/L, protein hydrolysate 2.2g/L.
(3) Fiber differentiation is shifted:
The callus that step (2) obtains is transferred to the second culture medium, and temperature is 27 ± 1 DEG C, with fluorescent lamp as light source,
Intensity of illumination control is 1800～2500Lx, and photoperiod control is the dark 10h of light 14h/, after callus induction goes out tender shoots, by tender
Bud intactly cuts from radix ranunculi ternati holder explant, continues with identical CMC model to formation in transferring to the 3rd culture medium
Radix ranunculi ternati seedling；
The constituent of described second culture medium with the addition of indolebutyric acid on described first medium base of step (2)
0.5～2.0mg/L and 2,4-dichlorobenzene oxygen butyl acetate 1.5～2.0mg/L.
The constituent of described 3rd culture medium with the addition of on described second medium base 6-benzyl aminopurine 1.5～
2.0mg/L。
(4) domestication of seedling and hardening:
After radix ranunculi ternati seedling replanting to hardening matrix, from induced environment, every day reduces temperature 1 DEG C, intensity of illumination every day
Increase 300Lx, to when identical with external environment condition, be further cultured for 2～3 days can transplanting to field to be broadcast.
The constituent of described hardening matrix is: perlite, vermiculite, sawdust, pine bark, sphagna, flower mud, peat soil and
Fused calcium magnesium phosphorus potash fertilizer, its mass ratio is 1:1:0.5:0.5:0.3:0.1:0.1:0.01.
(5) transplant:
Selecting soil layer hillside deep, well-drained or wasteland as field to be broadcast, thin after-applied base fertilizer is ploughed in leveling, in step
(4), after the seedling described in adapts to external environment condition normal growth, transplant to described field to be broadcast, line-spacing 10～15cm, spacing in the rows 5～8cm.
Described base fertilizer includes radix ranunculi ternati Special slow release fertilizer, and described radix ranunculi ternati Special slow release fertilizer forms and parts by weight are: urine
Element 100 parts, 20～25 parts of ammonium hydrogen carbonate, 12～14 parts of ammonium hydrogen carbonate, 3～6 parts of calcium nitrate, Diammonium phosphate (DAP) 50～60 parts, calcium and magnesium
12～14 parts of phosphate fertilizer, potassium sulfate 50～60 parts, zinc ammonium phosphate 3～5 parts, potassium silicate 4～7 parts, borax 1～3 part, manganese sulfate 1～3
Part.
(6) field management:
Carry out in time in growth period loosening the soil, water, the Routine Management such as weeding；Pest management aspect, for ensureing radix ranunculi ternati
Chinese medicine characteristic, agricultural chemicals is not applied to the insect pest that general harmfulness is little, to disease then to put prevention first, after disease occurs
Chinese medicine sterilizing liquid is used to carry out sterilization processing to radix ranunculi ternati.
(7) results and seed collecting:
After radix ranunculi ternati cultivation year and a day, digging out the block root of its under ground portion root, the seed of aerial part is adopted
Collection, completes artificial cultivation and the breeding of radix ranunculi ternati simultaneously.
Claims (8)
1. the artificial cultivation method of a radix ranunculi ternati, it is characterised in that: specifically comprise the following steps that
(1) holder explant pretreatment:
Choose the bud of radix ranunculi ternati, after refrigerating 3～5 days in 4 DEG C of environment, by bud taking-up clear-cutting forestland to room temperature, peel off bud
Piece and little Hua, rinse with pure water, and first sterilize in the mercuric chloride aqueous solution of 0.05% 5～8min, then with 70% alcohol disinfecting
20～40s, uses aseptic water washing 3 times afterwards, i.e. obtains radix ranunculi ternati holder explant；The percentage that above is referred to is volume hundred
Proportion by subtraction；
(2) explant inoculation and Fiber differentiation:
In the first culture medium after radix ranunculi ternati holder explant is moved into sterilizing, temperature is set as 23 ± 1 DEG C, with blue-ray light
For light source, intensity of illumination control is 400～800Lx, and photoperiod control is the dark 10h of light 14h/, induces callus；
(3) Fiber differentiation is shifted:
The callus that step (2) obtains is transferred to the second culture medium, and temperature is 27 ± 1 DEG C, with fluorescent lamp as light source, illumination
Strength control is 1800～2500Lx, the photoperiod control be the dark 10h of light 14h/, after callus induction goes out tender shoots, by tender shoots from
Intactly cut on radix ranunculi ternati holder explant, transfer to continue in the 3rd culture medium with identical CMC model to forming ram's horn
Grass seedling；
(4) domestication of seedling and hardening:
By in radix ranunculi ternati seedling replanting to hardening matrix, and progressively transfer in external environment condition；
(5) transplant:
Selecting soil layer hillside deep, well-drained or wasteland as field to be broadcast, thin after-applied base fertilizer is ploughed in leveling, in step (4)
After described seedling adapts to external environment condition normal growth, transplant to described field to be broadcast, line-spacing 10～15cm, spacing in the rows 5～8cm；
(6) field management:
Carry out in time in growth period loosening the soil, water, the Routine Management such as weeding；Pest management aspect, for ensureing in radix ranunculi ternati
Medicine characteristic, not applies agricultural chemicals to the insect pest that general harmfulness is little, to disease then to put prevention first, uses after disease occurs
Chinese medicine sterilizing liquid carries out sterilization processing to radix ranunculi ternati；
(7) results and seed collecting:
After radix ranunculi ternati cultivation year and a day, digging out the block root of its under ground portion root, the seed of aerial part is gathered, with
When complete artificial cultivation and the breeding of radix ranunculi ternati.
2. the artificial cultivation method of radix ranunculi ternati according to claim 1, it is characterised in that: step (2) described first is cultivated
The constituent of base is: KNO3800～1300mg/L, 2-amino-5-carboxyl pentanamide 800～1200mg/L, KH2PO4 300
～450mg/L, CaCl2·2H2O 320～350mg/L, MgSO4·7H2O 180～240mg/L, EDTA120～180mg/L,
FeSO4·7H2O 30～50mg/L, MnSO4·4H2O 50～80mg/L, ZnSO4·7H2O 15～30mg/L, H3BO38～
10mg/L, KI 5～10mg/L, Na2MoO4·2H2O 1～5mg/L, CoCl2·6H2O 0.2～0.5mg/L, 4-iodobenzene oxygen second
Acid 0.15～0.20mg/L, inositol 30～80mg/L, glycine 0.5～1.0mg/L, vitamin B1 0.5～0.75mg/L, dimension
Raw element B6 1.0～1.5mg/L, nicotinic acid 1.0～1.5mg/L, sucrose 30～50g/L, agar 10～15g/L, kinetin KT 0.5
～1.0mg/L, methyl α-naphthyl acetate NAA0.3～0.4mg/L, sorbierite 50～70g/L, biotin 0.15～0.20mg/L, hydrochloric acid pyrrole is trembled
Alcohol 0.5～1.0mg/L, thiamine hydrochloride 0.5～1.0mg/L, methyl-nitroso-urea 1.5～2.0mg/L, indolebutyric acid 1.5～
2.0mg/L, alanine 5～10mg/L, folic acid 5～10mg/L, Prohexadione calcium 1.5～2.0mg/L, colchicin 1.5～
2.0mg/L, protein hydrolysate 2.0～2.5g/L.
3. the artificial cultivation method of a kind of radix ranunculi ternati according to claim 1 and 2, it is characterised in that: described second cultivates
The constituent of base with the addition of indolebutyric acid 0.5～2.0mg/L and 2,4-dichloro on described first medium base of step (2)
Phenoxy acetic acid butyl ester 1.5～2.0mg/L；
The constituent of described 3rd culture medium with the addition of on described second medium base 6-benzyl aminopurine 1.5～
2.0mg/L。
4. the artificial cultivation method of a kind of radix ranunculi ternati according to claim 1, it is characterised in that: the described hardening of step (4)
The constituent of matrix is: perlite, vermiculite, sawdust, pine bark, sphagna, flower mud, peat soil and fused calcium magnesium phosphorus potash fertilizer.
5. the artificial cultivation method of a kind of radix ranunculi ternati according to claim 4, it is characterised in that: in described hardening matrix,
Perlite, vermiculite, sawdust, pine bark, sphagna, flower mud, peat soil, the mass ratio of fused calcium magnesium phosphorus potash fertilizer be 1:0.5～1.5:0.2～
0.8:0.2～0.8:0.1～0.5:0.05～0.15:0.05～0.15:0.005～0.015.
6. the artificial cultivation method of a kind of radix ranunculi ternati according to claim 5, it is characterised in that: in described hardening matrix,
Perlite, vermiculite, sawdust, pine bark, sphagna, flower mud, peat soil, the mass ratio of fused calcium magnesium phosphorus potash fertilizer are 1:1:0.5:0.5:0.3:
0.1:0.1:0.01。
7. the artificial cultivation method of a kind of radix ranunculi ternati according to any one of claim 1～6, it is characterised in that: step (5)
Described base fertilizer includes radix ranunculi ternati Special slow release fertilizer, and described radix ranunculi ternati Special slow release fertilizer forms and parts by weight are: 100 parts of urea,
Polysaccharide 50～100 parts, 20～25 parts of ammonium hydrogen carbonate, 12～14 parts of ammonium hydrogen carbonate, 3～6 parts of calcium nitrate, Diammonium phosphate (DAP) 50～60
Part, 12～14 parts of calcium magnesium phosphate, potassium sulfate 50～60 parts, zinc ammonium phosphate 3～5 parts, potassium silicate 4～7 parts, borax 1～3 part, sulphur
1～3 part of manganese of acid.
8. the artificial cultivation method of a kind of radix ranunculi ternati according to claim 7, it is characterised in that: the weight of described polysaccharide
Number is 65 parts.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107980458A (en) * | 2017-12-10 | 2018-05-04 | 广西长耀隆农业科技有限公司 | A kind of Bougainvillea spectabilis cuttage breeding method |
| CN107996401A (en) * | 2017-11-30 | 2018-05-08 | 安徽心缘康生物科技有限公司 | A kind of peony tissue culture prevent-browning promotees root-growing agent |
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