CN106106170A - A kind of Rhizoma Paridis method for quickly breeding - Google Patents
A kind of Rhizoma Paridis method for quickly breeding Download PDFInfo
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- CN106106170A CN106106170A CN201610583996.2A CN201610583996A CN106106170A CN 106106170 A CN106106170 A CN 106106170A CN 201610583996 A CN201610583996 A CN 201610583996A CN 106106170 A CN106106170 A CN 106106170A
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- rhizoma paridis
- rhizome
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of Rhizoma Paridis method for quickly breeding, the tuber that mainly present in solution prior art, existing Rhizoma Paridis tuber modes of reproduction exists can only breed a sprout, the root formed is less, poor growth, it is impossible to meet the existing market problem to the demand that Rhizoma Paridis is bred.This selection is perennial, healthy and strong, not damaged, without the Rhizoma Paridis rhizome of pest and disease damage, as breeding kind source；Then by fresh for Rhizoma Paridis rhizome dig out clean after dry, excision rhizome on adventitious root；Then, after stipes aseptic water washing rear cutout being removed sprout thereon, above-mentioned rhizome stipes is cut into block structure；Then block structure is accessed in propagating culture medium and cultivate；Grow rough leaf in block structure, when adventitious root grows 5, be transplanted in soft soil growth.By such scheme, invention has reached Fast-propagation Rhizoma Paridis, is that Rhizoma Paridis is taken root more purpose.
Description
Technical field
The present invention relates to Rhizoma Paridis field of planting, specifically, relate to a kind of Rhizoma Paridis method for quickly breeding.
Background technology
Rhizoma Paridis is mainly used in the effects such as reducing swelling and alleviating pain, anticancer, heat-clearing and toxic substances removing and hemostasis, has snake bite and insect sting, traumatic injury
Unique curative effect, both can directly be used as medicine, be again the multiple Chinese patent medicines such as Ji Shengde antivenom tablet, YUNNAN BAIYAO, the cleer and peaceful GONGXUENING of pyretic toxicity
One of primary raw material bucket；Owing to Rhizoma Paridis is perennial plant, and growing environment is required higher, after additionally Rhizoma Paridis seed has embryo
Ripe physiological property, will experience " secondary dormancy " phase, needs under field conditions (factors) could sprout through 2 years, and emergence rate is non-
The lowest, the natural speed of growth is very slow, only can not meet the market demand by wild resource breeding.
So far, quick breeding technology and the industrialization plantation of Rhizoma Paridis not yet achieves satisfactory results；In recent years
Rhizoma Paridis price continuous rise, directly results in and excavate wild Rhizoma Paridis predation formula, therefore exploitation Rhizoma Paridis quick breeding technology, is to solve
Certainly Rhizoma Paridis resource scarcity, ensure the key means of its sustainable use.
In prior art, Rhizoma Paridis uses tuber modes of reproduction, but one tuber of this mode can only breed a sprout, shape
The root become is less, poor growth；And current rhizoma paris rhizome fresh goods about 80-110 unit/kg, every mu needs 80kg rhizoma paris rhizome, every mu of ground
Seedling cost just reaches 8000 yuan；After additionally Rhizoma Paridis only grows 3 years, effective ingredient Rhizoma Paridis saponin therein just can meet country
Standards of pharmacopoeia, and growing environment requires high, tuber to breed, and input is high, growth cycle is long, so existing Rhizoma Paridis propagation method
The most perfect.
Summary of the invention
The present invention provides a kind of Rhizoma Paridis method for quickly breeding, to solve the one of existing Rhizoma Paridis tuber modes of reproduction existence
Individual tuber can only breed a sprout, and the root of formation is less, poor growth, it is impossible to meets the demand that Rhizoma Paridis is bred by existing market
Problem.
In order to solve the problems referred to above, the invention following technical scheme of offer:
A kind of Rhizoma Paridis method for quickly breeding comprises the following steps:
(1) selecting perennial, healthy and strong, not damaged, without the Rhizoma Paridis rhizome that pest and disease damage fresh weight is more than 80g, as breeding
The source of kind；
(2) by fresh for the Rhizoma Paridis rhizome of step (1) dig out clean after dry in the air 3-7 days, the adventitious root on excision rhizome, then cut
Take 3-6 the stipes of band sprout；
(3) after 3-6 rear cutout of stipes aseptic water washing of step (2) being removed sprout thereon, by above-mentioned rhizome stipes
It is cut into the block structure of 3-5cm；
(4) block structure of step (3) is accessed cultivation in propagating culture medium；Propagating culture medium includes 1.0-4.0mg
L-1ABT1、0.1-1.0mg·L-1BA、200-500mg·L-1Biochemical fulvic acid potassium, is 6-15 DEG C, illumination every day in cultivation temperature
8-12 hour, intensity of illumination be to cultivate under conditions of 500-1500lx；
(5) block structure of step (4) grows rough leaf, when adventitious root grows more than 4, is transplanted to soft
Soil in grow.
Specifically, the propagating culture medium of step (4) also includes mesh medical stone powder, the 0.2-that 0.1-2% particle diameter is 50-60
Plant ash, the 0.1-0.5% of 1% are husky.
Specifically, the cultivation temperature transplanted to mellow soil in step (5) is 12-22 DEG C, relative humidity 75%-
85%, illumination every day 6-10 hour, intensity of illumination are 600-1200lx.
Compared with prior art, invention has the advantages that mesophytization potassium fulvate of the present invention and ABT1 mono-reinstate
Can achieve the effect that more preferably when Rhizoma Paridis tuber is bred, make adventitious bud induction frequency, bud ratio that Rhizoma Paridis tuber breeds, go out root
Survival rate after rate, transplanting is higher；In the present invention, medical stone powder and sand make propagating culture medium have certain permeability and make Rhizoma Paridis
Tuber can preferably grow, plant ash sterilization further to propagating culture medium, supplements the multiple ore deposit that Rhizoma Paridis tuber growth needs
It is the most sturdy that matter-element element makes that it grows.
Detailed description of the invention
Being described further invention below in conjunction with embodiment, the embodiment of invention includes but not limited to following enforcement
Example.
Embodiment 1
Selecting perennial, healthy and strong, not damaged, without the Rhizoma Paridis rhizome that pest and disease damage fresh weight is more than 80g, as breeding kind
Source；Then by fresh for Rhizoma Paridis rhizome dig out clean after dry in the air 3 days, the adventitious root on excision rhizome, then cut 3 stems of band sprout
Joint；Then, after 3 rear cutout of stipes aseptic water washing being removed sprout thereon, above-mentioned rhizome stipes is cut into the block knot of 3cm
Structure；Then block structure is accessed in propagating culture medium and cultivate；Propagating culture medium includes 1.0mg L-1ABT1、0.1mg·L- 1BA、200mg·L-1Biochemical fulvic acid potassium, 0.1% particle diameter are the mesh medical stone powder of 50, the plant ash of 0.2%, 0.1% husky,
Cultivation temperature is 6 DEG C, illumination every day 8 hours, intensity of illumination are to cultivate under conditions of 500x；First is grown in block structure
Sheet true leaf, when adventitious root grows 4, is transplanted in soft soil growth；Cultivation temperature in mellow soil is 12 DEG C,
Relative humidity 75%, illumination every day 6 hours, intensity of illumination are 600x.
Embodiment 2
Selecting perennial, healthy and strong, not damaged, without the Rhizoma Paridis rhizome that pest and disease damage fresh weight is more than 80g, as breeding kind
Source；Then by fresh for Rhizoma Paridis rhizome dig out clean after dry in the air 4 days, the adventitious root on excision rhizome, then cut 4 stems of band sprout
Joint；Then, after 4 rear cutout of stipes aseptic water washing being removed sprout thereon, above-mentioned rhizome stipes is cut into the bulk of 3.5cm
Structure；Then block structure is accessed in propagating culture medium and cultivate；Propagating culture medium includes 2.0mg L-1ABT1、0.3mg·L-1BA、300mg·L-1Biochemical fulvic acid potassium, 0.5% particle diameter are the mesh medical stone powder of 55, the plant ash of 0.5%, 0.2% husky,
Cultivation temperature is 10 DEG C, illumination every day 10 hours, intensity of illumination are to cultivate under conditions of 8000x；Grow in block structure
Rough leaf, when adventitious root grows 5, is transplanted in soft soil growth；Cultivation temperature in mellow soil is
18 DEG C, relative humidity 78%, illumination every day 8 hours, intensity of illumination be 10000x.
Embodiment 3
Selecting perennial, healthy and strong, not damaged, without the Rhizoma Paridis rhizome that pest and disease damage fresh weight is more than 80g, as breeding kind
Source；Then by fresh for Rhizoma Paridis rhizome dig out clean after dry in the air 5 days, the adventitious root on excision rhizome, then cut 5 stems of band sprout
Joint；Then, after 5 rear cutout of stipes aseptic water washing being removed sprout thereon, above-mentioned rhizome stipes is cut into the block knot of 4cm
Structure；Then block structure is accessed in propagating culture medium and cultivate；Propagating culture medium includes 3.0mg L-1ABT1、0.7mg·L- 1BA、400mg·L-1Biochemical fulvic acid potassium, 1.5% particle diameter are the mesh medical stone powder of 58, the plant ash of 0.8%, 0.4% husky,
Cultivation temperature is 12 DEG C, illumination every day 11 hours, intensity of illumination are to cultivate under conditions of 10000x；Grow in block structure
Rough leaf, when adventitious root grows 6, is transplanted in soft soil growth；Cultivation temperature in mellow soil is
21 DEG C, relative humidity 83%, illumination every day 9 hours, intensity of illumination be 12000x.
Embodiment 4
Selecting perennial, healthy and strong, not damaged, without the Rhizoma Paridis rhizome that pest and disease damage fresh weight is more than 80g, as breeding kind
Source；Then by fresh for Rhizoma Paridis rhizome dig out clean after dry in the air 7 days, the adventitious root on excision rhizome, then cut 6 stems of band sprout
Joint；Then, after 6 rear cutout of stipes aseptic water washing being removed sprout thereon, above-mentioned rhizome stipes is cut into the block knot of 5cm
Structure；Then block structure is accessed in propagating culture medium and cultivate；Propagating culture medium includes 4.0mg L-1ABT1、0.1mg·L- 1BA、500mg·L-1Biochemical fulvic acid potassium, 2% particle diameter are the mesh medical stone powder of 60, the plant ash of 1%, 0.5% husky, are cultivating
Temperature is 15 DEG C, illumination every day 12 hours, intensity of illumination are to cultivate under conditions of 15001x；First is grown in block structure
Sheet true leaf, when adventitious root grows 7, is transplanted in soft soil growth；Cultivation temperature in mellow soil is 22 DEG C,
Relative humidity 85%, illumination every day 10 hours, intensity of illumination are 12001x.
Embodiment 5
Selecting perennial, healthy and strong, not damaged, without the Rhizoma Paridis rhizome that pest and disease damage fresh weight is more than 80g, as breeding kind
Source；Then by fresh for Rhizoma Paridis rhizome dig out clean after dry in the air 7 days, the adventitious root on excision rhizome, then cut 6 stems of band sprout
Joint；Then, after 6 rear cutout of stipes aseptic water washing being removed sprout thereon, above-mentioned rhizome stipes is cut into the block knot of 5cm
Structure；Then block structure is accessed in propagating culture medium and cultivate；Propagating culture medium includes 4.0mg L-1ABT1、0.1mg·L- 1BA, 2% particle diameter are the mesh medical stone powder of 60, the plant ash of 1%, 0.5% husky, cultivation temperature be 15 DEG C, illumination every day 12 little
Time, intensity of illumination be to cultivate under conditions of 15001x；Rough leaf is grown in block structure, when adventitious root grows 7,
It is transplanted in soft soil growth；Cultivation temperature in mellow soil is 22 DEG C, relative humidity 85%, illumination every day 10
Hour, intensity of illumination be 12001x.
Embodiment 6
Selecting perennial, healthy and strong, not damaged, without the Rhizoma Paridis rhizome that pest and disease damage fresh weight is more than 80g, as breeding kind
Source；Then by fresh for Rhizoma Paridis rhizome dig out clean after dry in the air 7 days, the adventitious root on excision rhizome, then cut 6 stems of band sprout
Joint；Then, after 6 rear cutout of stipes aseptic water washing being removed sprout thereon, above-mentioned rhizome stipes is cut into the block knot of 5cm
Structure；Then block structure is accessed in propagating culture medium and cultivate；Propagating culture medium includes 0.1mg L-1BA、500mg·L-1Raw
Change mesh medical stone powder that potassium fulvate, 2% particle diameter are 60, the plant ash of 1%, 0.5% husky, be 15 DEG C, every day in cultivation temperature
Illumination 12 hours, intensity of illumination are to cultivate under conditions of 15001x；Growing rough leaf in block structure, adventitious root is raw
When long 7, it is transplanted in soft soil growth；Cultivation temperature in mellow soil is 22 DEG C, relative humidity 85%, every
It illumination 10 hours, intensity of illumination are 12001x.
Checked embodiment 1,2,3,4,5,6 and the adventitious bud induction frequency of existing propagation method, bud ratio through 25 days, sprout
Rate, transplant to the survival rate of mellow soil, by take out the tuber of equal number by the method for averaging draw above-mentioned respectively
The value of parameter；Concrete condition such as following table:
Interpretation of result: the adventitious bud induction frequency of embodiment 1 to embodiment 4, bud ratio, go out root rate, transplant after survival rate
The most close, not there is marked difference, and its all than existing propagation method adventitious bud induction frequency, bud ratio, go out root rate, transplant after
Survival rate high, therefore the most existing propagation method of the propagation method of the present invention has significant advantage；Embodiment 5 and embodiment 6
Adventitious bud induction frequency, bud ratio, go out root rate, transplant after survival rate be below embodiment 1 to embodiment 4, embodiment 5 is the most real
Executing example 1 to embodiment 4 and lacked biochemical fulvic acid potassium, relative embodiment 1 to the embodiment 4 of embodiment 6 has lacked ABT1；Therefore it is biochemical
Potassium fulvate can achieve the effect that more preferably together with ABT1 when Rhizoma Paridis tuber is bred, and make that Rhizoma Paridis tuber breeds is indefinite
Bud induction rate, bud ratio, go out root rate, transplant after survival rate higher；In the present invention, medical stone powder and sand make propagating culture medium have
Certain permeability is had to make Rhizoma Paridis tuber preferably to grow, plant ash sterilization further to propagating culture medium, supplements Rhizoma Paridis
It is the most sturdy that the several kinds of mineral elements that tuber growth needs makes that it grows.
According to above-described embodiment, invention just can be realized well.What deserves to be explained is, premise based on said structure design
Under, for solving same technical problem, even if some made in invention are without substantial change or polishing, the skill used
The essence of art scheme is still as invention, therefore it should also be as in the protection domain of invention.
Claims (3)
1. a Rhizoma Paridis method for quickly breeding, it is characterised in that comprise the following steps:
(1) selecting perennial, healthy and strong, not damaged, without the Rhizoma Paridis rhizome that pest and disease damage fresh weight is more than 80g, as a breeding kind source；
(2) by fresh for the Rhizoma Paridis rhizome of step (1) dig out clean after dry in the air 3-7 days, the adventitious root on excision rhizome, then cut band
3-6 the stipes of sprout；
(3), after 3-6 rear cutout of stipes aseptic water washing of step (2) being removed sprout thereon, above-mentioned rhizome stipes is cut into
The block structure of 3-5cm；
(4) block structure of step (3) is accessed cultivation in propagating culture medium；Propagating culture medium includes 1.0-4.0mg L- 1ABT1、0.1-1.0mg·L-1BA、200-500mg·L-1Biochemical fulvic acid potassium, is 6-15 DEG C, illumination every day 8-in cultivation temperature
12 hours, intensity of illumination be to cultivate under conditions of 500-1500lx；
(5) block structure of step (4) grows rough leaf, when adventitious root grows more than 4, is transplanted to soft soil
Earth grows.
A kind of Rhizoma Paridis method for quickly breeding the most according to claim 1, it is characterised in that the breeding of step (4) is cultivated
Base also including, mesh medical stone powder that 0.1-2% particle diameter is 50-60, the plant ash of 0.2-1%, 0.1-0.5% are husky.
A kind of Rhizoma Paridis method for quickly breeding the most according to claim 1, it is characterised in that transplant to pine in step (5)
Cultivation temperature in weak soil earth is 12-22 DEG C, relative humidity 75%-85%, illumination every day 6-10 hour, intensity of illumination are 600-
1200lx。
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106417036A (en) * | 2016-12-21 | 2017-02-22 | 西南科技大学 | Tissue culture method for inducing immature zygotic embryos of paris polyphylla to generate somatic embryos |
| CN106613186A (en) * | 2016-12-01 | 2017-05-10 | 丽江鼎辉生物开发有限公司 | High-yield planting method of paris polyphylla |
| CN107155576A (en) * | 2017-05-23 | 2017-09-15 | 香格里拉市圣洁生物种植有限公司 | The method for culturing seedlings of Paris polyphylla seed |
| CN110432146A (en) * | 2019-08-30 | 2019-11-12 | 丽江思邈生物开发有限公司 | A kind of paris polyphylla nursery and large-scale planting method |
| CN110476818A (en) * | 2019-09-29 | 2019-11-22 | 中南林业科技大学 | A kind of auxiliary method for promoting tung oil tree stem with bud regeneration plant of physics |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444391A (en) * | 2013-07-31 | 2013-12-18 | 王小婵 | Cutting propagation and cultivation method for Paris polyphylla |
| CN104969742A (en) * | 2015-06-11 | 2015-10-14 | 怀化医学高等专科学校 | Rapid propagation method for cutting rhizomes of Paris polyphylla with high power |
| CN105104213A (en) * | 2015-10-15 | 2015-12-02 | 湖南农业大学 | Rapid propagation and seedling culture method using rhizomes of paris polyphylla |
| CN105210882A (en) * | 2015-11-05 | 2016-01-06 | 广西壮族自治区药用植物园 | A kind of tissue culture propagation of Diels Stephania Root |
| CN105325144A (en) * | 2015-10-19 | 2016-02-17 | 中国科学院昆明植物研究所 | Paris polyphylla rapid propagation technology |
-
2016
- 2016-07-22 CN CN201610583996.2A patent/CN106106170B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444391A (en) * | 2013-07-31 | 2013-12-18 | 王小婵 | Cutting propagation and cultivation method for Paris polyphylla |
| CN104969742A (en) * | 2015-06-11 | 2015-10-14 | 怀化医学高等专科学校 | Rapid propagation method for cutting rhizomes of Paris polyphylla with high power |
| CN105104213A (en) * | 2015-10-15 | 2015-12-02 | 湖南农业大学 | Rapid propagation and seedling culture method using rhizomes of paris polyphylla |
| CN105325144A (en) * | 2015-10-19 | 2016-02-17 | 中国科学院昆明植物研究所 | Paris polyphylla rapid propagation technology |
| CN105210882A (en) * | 2015-11-05 | 2016-01-06 | 广西壮族自治区药用植物园 | A kind of tissue culture propagation of Diels Stephania Root |
Non-Patent Citations (1)
| Title |
|---|
| 崔宝禄等: "重楼无性繁殖研究进展", 《河北林果研究》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613186A (en) * | 2016-12-01 | 2017-05-10 | 丽江鼎辉生物开发有限公司 | High-yield planting method of paris polyphylla |
| CN106417036A (en) * | 2016-12-21 | 2017-02-22 | 西南科技大学 | Tissue culture method for inducing immature zygotic embryos of paris polyphylla to generate somatic embryos |
| CN107155576A (en) * | 2017-05-23 | 2017-09-15 | 香格里拉市圣洁生物种植有限公司 | The method for culturing seedlings of Paris polyphylla seed |
| CN110432146A (en) * | 2019-08-30 | 2019-11-12 | 丽江思邈生物开发有限公司 | A kind of paris polyphylla nursery and large-scale planting method |
| CN110476818A (en) * | 2019-09-29 | 2019-11-22 | 中南林业科技大学 | A kind of auxiliary method for promoting tung oil tree stem with bud regeneration plant of physics |
| CN110476818B (en) * | 2019-09-29 | 2020-08-14 | 中南林业科技大学 | Method for physically assisting regeneration of plant from tung tree stem with bud |
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|---|---|
| CN106106170B (en) | 2018-10-02 |
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