CN106105487A - A kind of method promoting Radix Gentianae Macrophyllae seed germination - Google Patents
A kind of method promoting Radix Gentianae Macrophyllae seed germination Download PDFInfo
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- CN106105487A CN106105487A CN201610718933.3A CN201610718933A CN106105487A CN 106105487 A CN106105487 A CN 106105487A CN 201610718933 A CN201610718933 A CN 201610718933A CN 106105487 A CN106105487 A CN 106105487A
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- seed
- radix gentianae
- biogas slurry
- gentianae macrophyllae
- treatment
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
Abstract
The invention discloses a kind of method promoting Radix Gentianae Macrophyllae seed germination, comprise the steps: 1) initial point selection: gather half ripe Radix Gentianae Macrophyllae seed, be placed in ventilated place, under the conditions of 25～30 DEG C, be dried to water content less than 15%；2) K cryogenic treatment: in seedling rearing room, after being mixed with wet sandy soil by dry seed, spreads on prefabricated seedbed, then covers with the mixture of wood flour and horsehit, controls seedling rearing room temperature 12～18 DEG C, carries out K cryogenic treatment 3～7 days；3) biogas slurry treatment: after K cryogenic treatment terminates, takes out and separates Radix Gentianae Macrophyllae seed, puts in aseptic biogas slurry, and room temperature processes 18～36 hours；4) after biogas slurry treatment, using 75% alcoholic solution to carry out disinfection, then tap water rinses 2～3 times.The present invention is simple to operate, with low cost, can be widely used.Germination after this method processes is fast, emerges neat, is obviously enhanced the resistance of low temperature and high temperature, has good market application foreground.
Description
Technical field
The invention belongs to Chinese crude drug planting technology field, particularly relate to a kind of method promoting Radix Gentianae Macrophyllae seed germination.
Background technology
Radix Gentianae Macrophyllae is Gentianaceae Gentiana herbaceos perennial, be grown on height above sea level 2000～4950m Subalpine region grass ten days and
Shrubbery.It is used as medicine with root, cold nature, acrid in the mouth, hardship；Return stomach warp, Liver Channel, gallbladder meridian；There are the effects such as rheumatism, relaxing muscles and tendons network, clearind deficient heat,
Cure mainly rheumatic arthralgia, the disease such as muscle arteries and veins contracture, joint are ached, afternoon fever, infantile malnutrition heating, in existing more than 2,000 year of China
Medicinal history.Owing to the most too much emphasizing the exploitation of Radix Gentianae Macrophyllae, make market demand quickly increase, cause excessively adopting
Dig, cause wild resource increasingly to reduce, be listed in three grades of national key protected plant of country.In order to alleviate its degree in imminent danger, protect the Qin
Macrophylla wild resource, artificial growth is gradually paid close attention to by people.Radix Gentianae Macrophyllae propagation method has seminal propagation and division propagation, seminal propagation
Plant strain growth in great numbers, rhizome is thick, is current most popular method；But its seed is little, the life-span is short, yield poorly, and after-ripening is had to make
With.No matter additionally, use and sow in spring or autumn sowing, in seeding process, all have that germination percentage is low, the situation such as irregular of germinateing, seriously
Govern standardized planting and the production of Radix Gentianae Macrophyllae.Tissue culture technology has been widely used in crop-planting technical field, but Radix Gentianae Macrophyllae
In tissue culture, due to seed skin depth and hard, conventionally cultivate aseptic seedling, germination rate and low, limit
Make the research utilizing genetic engineering means that Radix Gentianae Macrophyllae carries out improveing the aspects such as breeding.Therefore, for the extensive cultivation of Radix Gentianae Macrophyllae
Produce with standardization and utilize Radix Gentianae Macrophyllae seed to carry out tissue culture and genetic manipulation, when how research shortens Radix Gentianae Macrophyllae germination
Between, improve percentage of seedgermination and seed resistance and become the technical problem that this area is urgently to be resolved hurrily.
In recent years for solving germination problem, production generally uses the methods such as the process of chemicals treatment, the temperature difference, physical treatment.Examination
Test and show, in medicament method with gibberellins (Liu Lisha, Ji Keping, the research [J] of Radix Gentianae Macrophyllae germination characteristic. Chinese herbal medicine,
2002,3 (33): 269～271), NaCl, KNO3(different condition of culture and pretreatment are to the Qin for Teng Hongmei, Cao Xiaoyan, Wang Zhi
The impact [J] of Macrophylla seed germination. seed, 2008,11:87～91) etc. process, physical method are the ultrasonic place of 40kHz by frequency
Manage 15 minutes (Teng Hongmei, Cao Xiaoyan, Wang Zhi. different condition of culture and the pretreatment impact [J] on Radix Gentianae Macrophyllae seed germination. plant
Son, 2008,11:87～91), temperature difference method processes (Zhang Xiling, Wang Lan, Liu Lisha, gentiana straminea maxim with the hot-water soak of 20 DEG C
The research [J] of germination characteristic. Chinese crude drug, 2004,3 (27): 160～161) etc. have promote Radix Gentianae Macrophyllae seed germination work
With.But these methods still can not solve the problem in terms of shortening seed germinating time and improving resistance.To this end, patent CN
103250488 A disclose a kind of method promoting Radix Gentianae Macrophyllae seed germination, disclose and " are first immersed in by plant seed containing red mould
In the aqueous solution of element and ferrous sulfate, plant seed after being processed；Again the plant seed after described process is sowed, it is achieved promote
Enter plant seed germination and/or improve plant seed germination toleration；In the described aqueous solution containing gibberellins and ferrous sulfate
The mass ratio of gibberellins and ferrous sulfate is 1:5～200 ".It can effectively break stopping of seed by gibberellin treatment seed
Sleep, promote to sprout, help seed by the starting stage sprouted, induce seed vitality；Utilize certain density trace unit simultaneously
Element seed soaking, with the enzyme system in activated seed body, promotes the decomposition of seed storage material, accelerates the process of seed germination.Should
Method can make seed germination be significantly increased at germination percentage, germinating energy, germination index, and with often under 5 and 35 DEG C of sprouting conditions
Rule seed there are differences on germination percentage, germinating energy, germination index；It is embodied in germination fast, emerges neat, to low temperature and height
The resistance of temperature is obviously enhanced, and has good market application foreground.It is known that percentage of seedgermination is relevant to factors, and
Non-is hard with seed coat relevant, therefore, by said method can potential lifting germination percentage, stratification, but still be difficult to full
Foot demand.
Summary of the invention
It is an object of the invention to: a kind of method promoting Radix Gentianae Macrophyllae seed germination is provided, use " low temperature-biogas slurry-ethanol "
Combined Treatment means, Radix Gentianae Macrophyllae seed germination rate is low, weatherability is poor, sprout problem slowly in solution.
To achieve these goals, the present invention adopts the following technical scheme that
A kind of method promoting Radix Gentianae Macrophyllae seed germination, it is characterised in that: comprise the steps:
1) initial point selection: August 20 to 25, gathers half ripe Radix Gentianae Macrophyllae seed, is placed in ventilated place, at 25～30 DEG C of bars
Under part, being dried to water content less than 15%, wherein, seed requires full seed, without worm, and epidermis is smooth, free from insect pests；
2) K cryogenic treatment: in seedling rearing room, after dry seed is mixed according to the ratio of 1:8～10 with wet sandy soil, stand
It is placed on prefabricated seedbed, then covers with the mixture of wood flour with horsehit, cladding thickness 10～20cm, control seedling rearing room temperature 12
～18 DEG C, carrying out K cryogenic treatment, the time is 3～7 days；
3) biogas slurry treatment: after K cryogenic treatment terminates, takes out and separates Radix Gentianae Macrophyllae seed, puts in aseptic biogas slurry, and room temperature processes
18～36 hours；
4) after biogas slurry treatment, using 75% alcoholic solution to carry out disinfection, then tap water rinses 2～3 times.
Further, being additionally added gibberellins, and be thoroughly mixed in described aseptic biogas slurry, gibberellins addition is
0.01mg/L biogas slurry.
Further, described aseptic biogas slurry is additionally added FeSO4Solution, addition is 0.1ml/L biogas slurry.
Further, it is 6.4～7.2 that described aseptic biogas slurry pH value controls.
Further, described wood flour is with the mixture of horsehit, and wood flour part by weight is 65～78%.
Compared with prior art, the beneficial effects of the present invention is:
1) present invention carries out " K cryogenic treatment-biogas slurry immersion-alcohol disinfecting " integrated treatment prior to seeding to seed, especially
When K cryogenic treatment, after seed is mixed with wet sandy soil, spread on prefabricated seedbed, then cover with the mixture of wood flour with horsehit
Lid, controls seedling rearing room temperature 12～18 DEG C, carries out K cryogenic treatment, compares Conventional cryogenic treatment technology, the method use wood flour with
The mixture of horsehit covers, and breathability is more preferable, will not produce heap accumulated heat, it is ensured that seed internal and external temperature is stable, and closer to environment
Temperature；Then use room temperature biogas slurry immersion treatment, be easily formed the stable temperature difference, and then reach to promote the mesh of Radix Gentianae Macrophyllae seed germination
's.Additionally, the present invention selects half ripe seed, seed coat hardness is relatively low and water flow big, and nutrient distribution is unbalanced, adopts
When using K cryogenic treatment, it is possible to decrease water fluidity, and then make nutrition composition slowly mobile to equilibrium, and then improve the matter of seed
Amount, to improving, germination rate is favourable.
2) present invention is when utilizing biogas slurry immersion treatment, adds gibberellins and FeSO4Solution, mycin is that plant kingdom extensively deposits
Phytohormone, process seed with it and can effectively break the dormancy of seed, promote to sprout, help seed at the beginning of by sprouting
In stage beginning, induce seed vitality；For most of light seeds, Exogenous gibberellic acid can substitute for the dark of infrared induction seed
Sprout；Ferrous sulfate is commonly used for micro-fertilizer and applies seed, and ferrum is little at plant in-vivo content as the one of trace element,
But plant growing is indispensable.Certain density trace element seed soaking, can with the enzyme system in activated seed body,
Promote the decomposition of seed storage material, accelerate the process of seed germination.Therefore, the method using the present invention, make seed germination exist
Germination percentage, germinating energy, germination index are significantly increased.
3) present invention uses gibberellins-FeSO4Biogas slurry solution processes seed, compares the gibberellins-FeSO in conventional art4
Aqueous solution, it contains abundant nutritional labeling, and muddiness has corresponding viscosity, the most only seed germination to provide nutrient, is more easy to red
Mycin-FeSO4" fetter " in the surface of the seed, form one layer and promote to sprout nutrient film, improve seed to gibberellins and Fe2+Absorption,
Improve gibberellins and Fe2+Utilization rate, promote Radix Gentianae Macrophyllae seed germination effect more preferable.
4) present invention is simple to operate, with low cost, can be widely used.Germination after this method processes is fast,
Emerge neat, the resistance of low temperature and high temperature is obviously enhanced, there is good market application foreground.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme is described further, in order to same domain technical staff
Understanding.
Embodiment 1
A kind of method promoting Radix Gentianae Macrophyllae seed germination, comprises the steps:
1) initial point selection: August 20 to 25, gathers half ripe Radix Gentianae Macrophyllae seed, is placed in ventilated place, at 25～30 DEG C of bars
Under part, being dried to water content less than 15%, wherein, seed requires full seed, without worm, and epidermis is smooth, free from insect pests；
2) K cryogenic treatment: in seedling rearing room, after dry seed is mixed according to the ratio of 1:8～10 with wet sandy soil, stand
It is placed on prefabricated seedbed, then covers with the mixture of wood flour with horsehit, cladding thickness 10～20cm, control seedling rearing room temperature 12
～18 DEG C, carrying out K cryogenic treatment, the time is 3～7 days, and described wood flour is with the mixture of horsehit, and wood flour part by weight is 65
～78%；
3) biogas slurry treatment: after K cryogenic treatment terminates, takes out and separates Radix Gentianae Macrophyllae seed, puts in aseptic biogas slurry, and room temperature processes
18～36 hours, wherein, it was 6.4～7.2 that biogas slurry pH value controls；
4) after biogas slurry treatment, using 75% alcoholic solution to carry out disinfection, then tap water rinses 2～3 times.
Embodiment 2
A kind of method promoting Radix Gentianae Macrophyllae seed germination, comprises the steps:
1) initial point selection: August 20 to 25, gathers half ripe Radix Gentianae Macrophyllae seed, is placed in ventilated place, at 25～30 DEG C of bars
Under part, being dried to water content less than 15%, wherein, seed requires full seed, without worm, and epidermis is smooth, free from insect pests；
2) K cryogenic treatment: in seedling rearing room, after dry seed is mixed according to the ratio of 1:8～10 with wet sandy soil, stand
It is placed on prefabricated seedbed, then covers with the mixture of wood flour with horsehit, cladding thickness 10～20cm, control seedling rearing room temperature 12
～18 DEG C, carrying out K cryogenic treatment, the time is 3～7 days, and described wood flour is with the mixture of horsehit, and wood flour part by weight is 65
～78%；
3) biogas slurry treatment: after K cryogenic treatment terminates, takes out and separates Radix Gentianae Macrophyllae seed, puts in aseptic biogas slurry, and room temperature processes
18～36 hours, wherein, it was 6.4～7.2 that biogas slurry pH value controls；
4) after biogas slurry treatment, using 75% alcoholic solution to carry out disinfection, then tap water rinses 2～3 times, then uses
Broadcast under conventional method, and record germination rate.
Embodiment 2
A kind of method promoting Radix Gentianae Macrophyllae seed germination, comprises the steps:
1) initial point selection: August 20 to 25, gathers half ripe Radix Gentianae Macrophyllae seed, is placed in ventilated place, at 25～30 DEG C of bars
Under part, being dried to water content less than 15%, wherein, seed requires full seed, without worm, and epidermis is smooth, free from insect pests；
2) K cryogenic treatment: in seedling rearing room, after dry seed is mixed according to the ratio of 1:8～10 with wet sandy soil, stand
It is placed on prefabricated seedbed, then covers with the mixture of wood flour with horsehit, cladding thickness 10～20cm, control seedling rearing room temperature 12
～18 DEG C, carrying out K cryogenic treatment, the time is 3～7 days, and described wood flour is with the mixture of horsehit, and wood flour part by weight is 65
～78%；
3) biogas slurry treatment: after K cryogenic treatment terminates, takes out and separates Radix Gentianae Macrophyllae seed, puts in aseptic biogas slurry, to aseptic natural pond
Adding gibberellins in liquid, after being thoroughly mixed, gibberellins addition is 0.01mg/L biogas slurry, at normal temperatures, and immersion treatment 18
～36 hours, wherein, it is 6.4～7.2 that biogas slurry pH value controls；
4) after biogas slurry treatment, using 75% alcoholic solution to carry out disinfection, then tap water rinses 2～3 times, then uses
Broadcast under conventional method, and record germination rate.
Embodiment 3
A kind of method promoting Radix Gentianae Macrophyllae seed germination, comprises the steps:
1) initial point selection: August 20 to 25, gathers half ripe Radix Gentianae Macrophyllae seed, is placed in ventilated place, at 25～30 DEG C of bars
Under part, being dried to water content less than 15%, wherein, seed requires full seed, without worm, and epidermis is smooth, free from insect pests；
2) K cryogenic treatment: in seedling rearing room, after dry seed is mixed according to the ratio of 1:8～10 with wet sandy soil, stand
It is placed on prefabricated seedbed, then covers with the mixture of wood flour with horsehit, cladding thickness 10～20cm, control seedling rearing room temperature 12
～18 DEG C, carrying out K cryogenic treatment, the time is 3～7 days, and described wood flour is with the mixture of horsehit, and wood flour part by weight is 65
～78%；
3) biogas slurry treatment: after K cryogenic treatment terminates, takes out and separates Radix Gentianae Macrophyllae seed, puts in aseptic biogas slurry, to aseptic natural pond
Liquid adds FeSO4Solution, addition is 0.1ml/L biogas slurry, at normal temperatures, immersion treatment 18～36 hours, wherein, biogas slurry pH
Value control is 6.4～7.2；
4) after biogas slurry treatment, using 75% alcoholic solution to carry out disinfection, then tap water rinses 2～3 times, then uses
Broadcast under conventional method, and record germination rate.
Embodiment 4
A kind of method promoting Radix Gentianae Macrophyllae seed germination, comprises the steps:
1) initial point selection: August 20 to 25, gathers half ripe Radix Gentianae Macrophyllae seed, is placed in ventilated place, at 25～30 DEG C of bars
Under part, being dried to water content less than 15%, wherein, seed requires full seed, without worm, and epidermis is smooth, free from insect pests；
2) K cryogenic treatment: in seedling rearing room, after dry seed is mixed according to the ratio of 1:8～10 with wet sandy soil, stand
It is placed on prefabricated seedbed, then covers with the mixture of wood flour with horsehit, cladding thickness 10～20cm, control seedling rearing room temperature 12
～18 DEG C, carrying out K cryogenic treatment, the time is 3～7 days, and described wood flour is with the mixture of horsehit, and wood flour part by weight is 65
～78%；
3) biogas slurry treatment: after K cryogenic treatment terminates, takes out and separates Radix Gentianae Macrophyllae seed, puts in aseptic biogas slurry, to aseptic natural pond
Adding gibberellins in liquid, after being thoroughly mixed, gibberellins addition is 0.01mg/L biogas slurry；It is subsequently adding FeSO4Solution, adds
Entering amount is 0.1ml/L biogas slurry, and at normal temperatures, immersion treatment 18～36 hours, wherein, it is 6.4～7.2 that biogas slurry pH value controls；
4) after biogas slurry treatment, using 75% alcoholic solution to carry out disinfection, then tap water rinses 2～3 times, then uses
Broadcast under conventional method, and record germination rate.
In the above-described embodiments, broadcast under seed latter about 10 days and can start to germinate, be specifically shown in table 1 below.
The impact on germination rate of the table 1-different disposal
Knowable to upper table 1, processing Radix Gentianae Macrophyllae seed by technical solution of the present invention, sprout time all controls at about 11 days,
Particularly with the addition of gibberellins and FeSO4After solution, its sprout time is advanced to 9 days, and the sprout time comparing existing 10 days does sth. in advance 1
My god；Additionally, after being processed by the present invention, its germination rate improves to more than 81% from about 60%, especially add gibberellins and
FeSO4After solution, germination rate is up to 89.5%.
Claims (5)
1. the method promoting Radix Gentianae Macrophyllae seed germination, it is characterised in that: comprise the steps:
1) initial point selection: August 20 to 25, gathers half ripe Radix Gentianae Macrophyllae seed, is placed in ventilated place, 25～30 DEG C of conditions
Under, it is dried to water content less than 15%, wherein, seed requires full seed, without worm, and epidermis is smooth, free from insect pests；
2) K cryogenic treatment: in seedling rearing room, after being mixed according to the ratio of 1:8～10 with wet sandy soil by dry seed, spreads
On prefabricated seedbed, then cover with the mixture of wood flour with horsehit, cladding thickness 10～20cm, control seedling rearing room temperature 12～18
DEG C, carrying out K cryogenic treatment, the time is 3～7 days；
3) biogas slurry treatment: after K cryogenic treatment terminates, takes out and separates Radix Gentianae Macrophyllae seed, puts in aseptic biogas slurry, room temperature process 18～
36 hours；
4) after biogas slurry treatment, using 75% alcoholic solution to carry out disinfection, then tap water rinses 2～3 times.
A kind of method promoting Radix Gentianae Macrophyllae seed germination the most as claimed in claim 1, it is characterised in that: in described aseptic biogas slurry
Being additionally added gibberellins, and be thoroughly mixed, gibberellins addition is 0.01mg/L biogas slurry.
A kind of method promoting Radix Gentianae Macrophyllae seed germination the most as claimed in claim 1, it is characterised in that: in described aseptic biogas slurry
It is additionally added FeSO4Solution, addition is 0.1ml/L biogas slurry.
A kind of method promoting Radix Gentianae Macrophyllae seed germination the most as claimed in claim 1, it is characterised in that: described aseptic biogas slurry pH
Value control is 6.4～7.2.
A kind of method promoting Radix Gentianae Macrophyllae seed germination the most as claimed in claim 1, it is characterised in that: described wood flour and horsehit
Mixture in, wood flour part by weight is 65～78%.
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|---|---|---|---|
| CN201610718933.3A CN106105487A (en) | 2016-08-25 | 2016-08-25 | A kind of method promoting Radix Gentianae Macrophyllae seed germination |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1600056A (en) * | 2003-09-25 | 2005-03-30 | 中国科学院西北高原生物研究所 | Method for introducing and planting plants of twisted large leaf gentian root |
| CN103250488A (en) * | 2013-05-02 | 2013-08-21 | 中国农业大学 | Method for promoting seed germination of large-leaved gentian |
| CN105432295A (en) * | 2015-12-02 | 2016-03-30 | 宁蒗县国兵生物科技有限公司 | Gentiana macrophylla planting method for preventing successive cropping obstacles |
-
2016
- 2016-08-25 CN CN201610718933.3A patent/CN106105487A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1600056A (en) * | 2003-09-25 | 2005-03-30 | 中国科学院西北高原生物研究所 | Method for introducing and planting plants of twisted large leaf gentian root |
| CN103250488A (en) * | 2013-05-02 | 2013-08-21 | 中国农业大学 | Method for promoting seed germination of large-leaved gentian |
| CN105432295A (en) * | 2015-12-02 | 2016-03-30 | 宁蒗县国兵生物科技有限公司 | Gentiana macrophylla planting method for preventing successive cropping obstacles |
Non-Patent Citations (3)
| Title |
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| 张晓青: "大通地区秦艽的育苗技术", 《青海农林科技》 * |
| 张秋月: "沼液在作物浸种和催芽中的应用", 《河北农业科技》 * |
| 彭云霞 等: "不同成熟度秦艽种子的发芽特性", 《浙江农业科学》 * |
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Application publication date: 20161116 |