CN106083227A - A kind of culture medium of edible fungus containing Moringa and application thereof - Google Patents

A kind of culture medium of edible fungus containing Moringa and application thereof Download PDF

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Publication number
CN106083227A
CN106083227A CN201610401771.0A CN201610401771A CN106083227A CN 106083227 A CN106083227 A CN 106083227A CN 201610401771 A CN201610401771 A CN 201610401771A CN 106083227 A CN106083227 A CN 106083227A
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moringa
culture medium
edible fungus
bar
raw material
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CN106083227B (en
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吴圣进
吴小建
沙国新
韦仕岩
陈雪凤
王灿琴
陈丽新
陈振妮
唐军
郎宁
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Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
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INSTITUTE OF MICROBIOLOGY GUANGXI ZHUANG AUTONOMOUS ACADEMY OF AGRICULTURAL SCIENCES
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention discloses a kind of culture medium of edible fungus containing Moringa, this culture medium includes following raw material by weight percentage: Moringa bar 60~100%, leaf of Moringa 0~40%, Moringa pod 0~15%.The invention also discloses the purposes of this culture medium.The present invention uses Moringa garbage to be major ingredient culturing edible fungus; belong to and first Moringa is used for edible fungus culturing; alleviate Moringa bar utilization ways few; utilization rate is low; the situation of difficult treatment, organically combines Moringa plantation and the big industry of edible fungus culturing two simultaneously, on the one hand avoids a large amount of Moringa bar wasting of resources; on the other hand the problem also alleviating current edible fungus culturing raw material worsening shortages, for protection environment, builds many Cyclic Economies body and is significant.

Description

A kind of culture medium of edible fungus containing Moringa and application thereof
Technical field
The present invention relates to technical field of edible fungi production, a kind of culture medium of edible fungus containing Moringa and Its purposes.
Background technology
Edible fungi is global health food, is liked by common people, along with people with rich in proteins, vitamins Growth in the living standard, the demand of edible fungi increases, the demand growing for meeting people, it is necessary to greatly develop edible fungi Production.Because Edible Fungi needs to consume substantial amounts of broad leaf tree resource, greatly develop the production of edible fungi necessarily in region And the quantity of the forest reserves of surrounding area and quality, the particularly quantity of natural broad-leaved forests resource and quality bring huge Impact.Along with Environmental protection is increasingly paid attention to, the dynamics of protection forest tree resource continues to increase, and particularly wildwood protects The progressively operation of nurse's journey, increases the protection to the forest reserves, and wood producing amount declines year by year, and the rotten class edible fungus culturing of wood is former Material supply be becoming tight day, raw material source and be supplied in the bottleneck grown for whole industry development.The most correctly process The mushroom industry gone from strength to strength well and the raw material supply contradiction of growing tension, Development of Novel alternative materials, promote regional economy The important problem that mushroom industry is badly in need of solving at present has been become with Coordination Development Between Eco-environment.
Moringa is Moringaceae (Moringaceae) Moringa (MoringaAdans) plant, originates in north India, non- The ground such as continent, south subtropics, has plurality of health care functions.India is Moringa manufacturing country maximum in the world at present, cultivated area 38000hm2, annual per hectare income, up to 1500 dollars, has people more than 5.2 ten thousand to be engaged in cultivating Moringa oleifera, is better economic benefit Crop.China introduces Moringa the sixties in 20th century, and at present, whole nation Moringa cultivated area is about about 40,000 mu, and only Yunnan just reaches About 20000 mu, accounting for the 50% of whole nation Moringa cultivated area, Moringa industry development in recent years is swift and violent, has become as China and breeds In new industry.
Moringa is perennial fast-growing tree, and growth is exceedingly fast, and within 1 year, its diameter increases averagely up to 5cm, maximum up to 10cm, raw Thing amount is huge.The Different Organs of Moringa Herb all can develop, the equal edible of blade, pod, tender shoots, flower, tender stem and root.Though So the various piece of Moringa has value of exploiting and utilizing, but the current development and utilization to Moringa is also in reduced levels, country Relevant laws and regulations the most only allow Moringa blade to eat.A large amount of stems, branch that thus Moringa plantation produces not only can not get Utilize, become Moringa plantation, the burden of processing enterprise on the contrary.Cultivating Moringa oleifera edible fungi is utilized on the one hand to solve Moringa industry big Amount stem, branch can not get effectively utilizing, and cause the problem that ample resources is wasted.On the other hand mushroom industry is alleviated the tightest The raw material supply problem opened, for building recycling economy, it is achieved two big industries develop jointly, developing agricultural ecological circulation economy has Significant.At present, there is not been reported to utilize the correlational study of cultivating Moringa oleifera edible fungi.
Summary of the invention
For above-mentioned technical problem, the present inventor, through performing creative labour, has invented a kind of food containing Moringa With bacterium culture medium, and disclosing the preparation method and its usage of this culture medium, this culture medium need not add other raw materials again, Formula is simple, and cost of material is low, and input-output ratio is high, and economy and environmental benefit are fairly obvious.
For achieving the above object, the technical solution adopted in the present invention is:
A kind of culture medium of edible fungus containing Moringa, this culture medium includes following raw material by weight percentage: Moringa bar 60 ~100%, leaf of Moringa 0~40%, Moringa pod 0~15%.
The above is containing culture medium of edible fungus of Moringa, preferably includes following raw material by weight percentage: Moringa bar 75 ~100%, leaf of Moringa 0~25%, Moringa pod 0~10%.
The above culture medium of edible fungus containing Moringa, from cost consideration, the most further preferably includes Following raw material: Moringa bar 100%.
The above is containing culture medium of edible fungus of Moringa, the most most preferably preferably includes following raw material: Moringa Bar 85%, leaf of Moringa 15%.
Described Moringa bar is: take stem and/or the branch of Moringa, through drying or drying, pulverizes, to obtain final product;Preferably can pass through 5 mesh The Moringa bar of sieve;Described leaf of Moringa is: take leaf of Moringa, through drying or drying, pulverizes, to obtain final product;Described Moringa pod is: take Moringa Pod, takes out the fruit pod shell after seed for behen-nut pod, through drying or drying, pulverizes, to obtain final product.
The preparation method of more than one described culture medium of edible fungus containing Moringa, comprises the following steps:
Weigh following raw material the most respectively: Moringa bar 60~100%, leaf of Moringa 0~40%, Moringa pod 0 ~15%.
2. add water damping by Moringa bar, fermentation 3~8 days of at room temperature banking up, and adds leaf of Moringa, Moringa pod, mix homogeneously;
3. controlling gained moisture content in medium is 60%~68%, and pH value is 8.0~8.5, and sterilizing to obtain final product.
The most described Moringa bar adds water fermentation 5-6 days of banking up after damping, banks up turning in the 3rd day once.
Amount of water in the above step 2 should be less than above-mentioned raw materials weight sum, the most just can be by described Moringa bar Complete wetting;Described room temperature is 10~30 DEG C, preferably 25 ± 2 DEG C.
The above preparation method of culture medium of edible fungus containing Moringa, the sterilizing described in step 3, can use normal pressure or The modes such as autoclaving, such as the damp and hot high-pressure sterilizing method of " 121 DEG C, sterilizing 1.5-2 hour ".Preferably normal-pressure sterilization;The most excellent The sterilizing methods of choosing is 100 DEG C of normal-pressure sterilizations 10~12 hours.
The purposes of above-described culture medium of edible fungus, described culture medium of edible fungus can be used for the cultivation of various edible fungi Training;Described edible fungi is preferably Pleurotus ostreatus, Auricularia polytricha (Mout) Sacc. or JIGU;Described edible fungi is most preferably preferably Pleurotus ostreatus.
Compared with prior art, the invention have the benefit that
The culture medium of edible fungus formula of the present invention is simple, the main garbage culturing edible fungus using Moringa, it is not necessary to add Add the convenient source such as cotton seed hulls, wood flour and the adjuvant such as Testa Tritici, Semen Maydis powder, reduce Edible Fungi cost, also belong to first simultaneously Moringa being used for edible fungus culturing, alleviates Moringa tree bar utilization ways few, utilization rate is low, the situation of difficult treatment, and will Moringa plantation and the big industry of edible fungus culturing two organically combine, and on the one hand avoid the resource wave of a large amount of Moringa tree bar, Moringa pod Take, the problem on the other hand also alleviating current edible fungus culturing raw material worsening shortages, for protection environment, build many industries and follow Ring economy is significant.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.
One, the preparation of culture medium of edible fungus cultivation
Moringa bar, leaf of Moringa, Moringa pod are provided by Guangxi Xi Yuan Moringa company limited.
Described Moringa bar is: take the stem of Moringa, branch, through drying, pulverizes, crosses 5 mesh sieves, to obtain final product.
Described leaf of Moringa is: take leaf of Moringa, through drying, pulverizes, to obtain final product.
Described Moringa pod is: take the fruit pod shell after behen-nut pod takes out seed, through drying, pulverizes, to obtain final product.
Embodiment 1
The preparation method of the culture medium of edible fungus containing Moringa, comprises the following steps:
Weigh following raw material the most respectively: Moringa bar 100%;
2. the damping that added water by Moringa bar is mixed thoroughly, fermentation 3 days of banking up at 28-30 DEG C;
3. controlling gained moisture content in medium is 60%, and measuring pH value is 8.2, pack, every bag of 0.85kg, 100 DEG C of normal pressures Sterilizing 10h, to obtain final product.
Embodiment 2
The preparation method of the culture medium of edible fungus containing Moringa, comprises the following steps:
Weigh following raw material the most respectively: Moringa bar 85%, leaf of Moringa 15%;
2. add water damping by Moringa bar, fermentation 4 days of banking up at 25-27 DEG C, adds leaf of Moringa, mix homogeneously;
3. controlling gained moisture content in medium is 65%, and measuring pH value is 8.3, pack, every bag of 0.85kg, 100 DEG C of normal pressures Sterilizing 12h, to obtain final product.
Embodiment 3
The preparation method of the culture medium of edible fungus containing Moringa, comprises the following steps:
Weigh following raw material the most respectively: Moringa bar 65%, leaf of Moringa 25%, Moringa pod 10%;
2. add water damping by Moringa bar, fermentation 5 days of banking up at 20-23 DEG C, adds leaf of Moringa, Moringa pod, mix homogeneously;
3. control gained moisture content in medium be 68%, measure pH value be 8.0, pack, every bag of 0.85kg, put 121 DEG C, wet Heat sterilization 2 hours, to obtain final product.
Embodiment 4
The preparation method of the culture medium of edible fungus containing Moringa, comprises the following steps:
Weigh following raw material the most respectively: Moringa bar 60%, leaf of Moringa 40%;
2. add water damping by Moringa bar, bank up at 15-17 DEG C fermentation 6 days, bank up turning in the 3rd day once, fermentation 6 of banking up After it, add leaf of Moringa, mix homogeneously;
3. controlling gained moisture content in medium is 65%, and measuring pH value is 7.8, adds appropriate Calx and adjusts pH value to be 8.5, dress Bag, every bag of 0.85kg, 100 DEG C of normal-pressure sterilization 12h, to obtain final product.
Embodiment 5
The preparation method of the culture medium of edible fungus containing Moringa, comprises the following steps:
Weigh following raw material the most respectively: Moringa bar 75%, leaf of Moringa 10%, Moringa pod 15%;
2. add water damping by Moringa bar, fermentation 8 days of banking up at 10-12 DEG C, bank up the 3rd, 6 days respectively turning once, heap After putting fermentation 8 days, add leaf of Moringa, Moringa pod, mix homogeneously;
3. control gained moisture content in medium be 65%, measure pH value be 8.0, pack, every bag of 0.85kg, put 121 DEG C, wet Heat sterilization 2 hours, to obtain final product.
Two, the cultivation research of culture medium of edible fungus
Test material: the strains tested of research is Pleurotus ostreatus tea 39, by institute of microbiology of Guangxi academy of agricultural sciences the Breeding of Edible Mushroom Research department provides.Culture medium of edible fungus self-control used by reference examples, other materials is commercially.
The preparation method of reference examples culture medium of edible fungus is: weigh by weight percentage 82% Ramulus Mori bits, 15% Testa Tritici, 2% Calx, 1% Gypsum Fibrosum, Ramulus Mori bits are added water and mixes thoroughly, at room temperature bank up and after fermenting 3 days, add Testa Tritici, Calx and Gypsum Fibrosum stirring Uniformly, regulation moisture content in medium to 65%, pH to 8.2, pack, every bag of 0.85kg, 100 DEG C of normal-pressure sterilization 12h, to obtain final product.
1. test method
Prepared by 1.1 strains
Bacterial strain is Pleurotus ostreatus tea 39, conventionally prepares master clock, original seed and cultigen, the method be recorded in (Chang Mingchang, 2003, edible mushroom cultivation [M], Beijing: Chinese agriculture publishing house: 121-137).
Mother culture media: PDA culture medium: Rhizoma Solani tuber osi 200g, glucose 20g, agar 20g, add water 1000ml, and pH is natural.
Original seed, Cultivar culture medium: cotton seed hulls 82%, Testa Tritici 14%, Calx 2%, Gypsum Fibrosum 1%, calcium superphosphate 1%.
1.2 inoculation and Mycelium cultures
Inoculating at culture medium bag two according to sterile working's code, each embodiment and reference examples every batch make 100 bags of trainings Support base, be repeated 3 times test.After inoculation, bacterium bag being placed in baterial cultivation chamber and send out bacterium, room temperature controls at 25 DEG C~28 DEG C, relative air humidity It is 65%~70%.Observe mycelia growing state, if there being bacterium pocket infections miscellaneous bacteria to remove in time, record mycelial growth rate and Growing way.
1.3 management of producing mushroom with gather
Conventionally carry out management of producing mushroom and gather, concrete grammar be recorded in (Wang Hexiang, edible mushroom cultivation [M], Beijing: China Agricultyre University Press, 2008).
2. statistical method
2.1 mycelial growth rates and biological efficiency
In each culture medium prescription of observed and recorded, Growth of Pleurotus Mycelium speed (using simple interest measurement method), compares also simultaneously Record mycelium growth vigor and dense degree (with "+" number represent that mycelial density and growing way are strong and weak), first three tide mushroom of each formula added up in record Yield.
The per day speed of growth of mycelia (mm/d)=mycelium morphology factor (mm)/cultivated days (d)
Biological efficiency (%)=(fresh mushroom weight/siccative weight) × 100%
2.2 significant difference tests
Use duncan's new multiple range method to check between each formula whether to there is significant difference, with this, reliability of test is described.
3. test results and analysis
The impact on Growth of Pleurotus Mycelium of the 3.1 different culture media formula, refers to table 1.
Table 1 cultivating Moringa oleifera Experiment of Pleurotus Ostreatus different formulations mycelial growth situation
"+" number, represents that the dense degree of mycelia and growing way are strong and weak." A " and " a " represents significance of difference analysis, and same letter is said Between the reason of daylight, difference is the most notable.
As shown in Table 1, Pleurotus ostreatus 39 mycelia all shows preferable growing way in each culture medium, and mycelia is pure white dense, sturdy Effectively, grow vigorous.In 6 formula for examination, Pleurotus ostreatus tea 39 mycelia grows the soonest in the culture medium of embodiment 2, the denseest Close, test data is analyzed and significance of difference comparative result shows: Pleurotus ostreatus tea 39 mycelia is raw in embodiment 2 culture medium Long the fastest, growing way is best, but and between embodiment 1-5 and reference examples difference the most notable.
The impact on Yield of Pleurotus Ostreatus of the 3.2 different culture media formula, refers to table 2.
The table 2 different culture media formula Yield of Pleurotus Ostreatus significance of difference compares (duncan's new multiple range method)
Result shows, the biological efficiency of embodiment 4 is the highest, reaches 94.71%, is secondly embodiment 2, the most aobvious 1% In work level, reference examples is the most notable with the biological efficiency difference of embodiment 1-5.
The performance analysis that 3.3 different culture media formula produce for Pleurotus ostreatus, refers to table 3.
The performance analysis that table 3 different culture media formula produces for Pleurotus ostreatus
Note: the costs such as cost of labor, strain, sterilizing do not count;Pleurotus ostreatus fresh mushroom market procurement price is based on 6.0 yuan/kg;Cultivate Based raw material price (containing freight charges): 0.35 yuan/kg of Moringa bar;1.5 yuan/kg of leaf of Moringa;0.5 yuan/kg of Moringa pod;0.8 yuan of Ramulus Mori bits/ kg;2.6 yuan/kg of Testa Tritici;0.80 yuan/kg of Gypsum Fibrosum;0.40 yuan/kg of Calx.
From table 3, the compost cost of embodiment 1-5 is far below reference examples, the simultaneously net income of embodiment 1-5, throwing Enter output ratio and be above reference examples.Its main cause is that the Ramulus Mori bits that reference examples is used are high with Testa Tritici price, and embodiment institute The Moringa bar, the Moringa pod that use are very cheap, the expense that the mainly transport of its cost and pulverizing produce.
4. discuss
Result of the test shows, utilizes cultivating Moringa oleifera Pleurotus ostreatus, the normal fruiting of each formula all energy, wherein, Moringa wood flour 85%, peppery During wood leaf 15%, Growth of Pleurotus Mycelium situation is best, and mycelia is dense, and growing way is prosperous, and biological transformation ratio is high, and net income is high.Above-mentioned reality Execute the growing state of example 1-5, output condition is close with reference examples or is better than reference examples, and production cost is far below reference examples.
Owing to being limited by relevant food decree regulation and exploitation process technology, Moringa tree bar fails to obtain all the time Well utilize.Development recently as Moringa industry grows, and a large amount of Moringa tree bars of generation are difficult by, and processes difficulty Become puzzlement Moringa and plant a great problem of enterprise.This testing inspection finds that the nutritional labeling of Moringa tree bar is very comprehensive, thick egg Bai Hanliang is high, i.e. possesses the suitable carbon needed for culturing edible fungus/nitrogen ratio by adding the materials such as leaf of Moringa.Experiment in cultivation result is also Show: embodiment 1-5 in terms of mycelium growth vigor and biological conversion rate with the equal no significant difference of reference examples;Simultaneously because raw material becomes This is low, and the input-output ratio of embodiment 1-5 is far above reference examples.
The culture medium of edible fungus of the present invention is additionally operable to the experiment in cultivation of Auricularia polytricha (Mout) Sacc., JIGU, and result of the test shows: Auricularia polytricha (Mout) Sacc., JIGU growth potential is vigorous, sturdy, and biological efficiency reaches more than 85%, cost of material the most relatively market low cost, can be used for Auricularia polytricha (Mout) Sacc., The cultivation of JIGU.
Currently, utilizing agricultural organic waste culturing edible fungus is that one turns waste into wealth, and builds effective way of circular agriculture Footpath.Research and utilization cultivating Moringa oleifera edible fungi of the present invention, cheaper starting materials is easy to get, and culture material formula is simple, and cultivation effect is good.Pass through The application of the present invention, on the one hand avoids a large amount of Moringa tree bar wasting of resources, on the other hand also alleviates current edible fungus culturing The problem of raw material worsening shortages, is significant for protection environment construction many Cyclic Economies body, can be big on producing Try hard to recommend wide.
Above content is to combine concrete preferred implementation further description made for the present invention, it is impossible to assert Being embodied as of the present invention is confined to these explanations.For general technical staff of the technical field of the invention, Substitute or obvious modification without departing from making some equivalents on the premise of present inventive concept, and performance or purposes are identical, all should It is considered as belonging to the scope of patent protection that the present invention is determined by the claims submitted to.

Claims (7)

1. the culture medium of edible fungus containing Moringa, it is characterised in that include following raw material by weight percentage: Moringa bar 60 ~100%, leaf of Moringa 0~40%, Moringa pod 0~15%.
Culture medium of edible fungus containing Moringa the most according to claim 1, it is characterised in that include following by weight percentage Raw material: Moringa bar 75~100%, leaf of Moringa 0~25%, Moringa pod 0~10%.
Culture medium of edible fungus containing Moringa the most according to claim 1, it is characterised in that include following by weight percentage Raw material: Moringa bar 100%.
Culture medium of edible fungus containing Moringa the most according to claim 1, it is characterised in that include following by weight percentage Raw material: Moringa bar 85%, leaf of Moringa 15%.
5. according to the purposes of described culture medium of edible fungus arbitrary in Claims 1 to 4, it is characterised in that: described edible fungi Culture medium is for the cultivation of edible fungi.
The purposes of culture medium of edible fungus the most according to claim 5, it is characterised in that: described edible fungi is Pleurotus ostreatus, hair Auricularia or JIGU.
The purposes of culture medium of edible fungus the most according to claim 5, it is characterised in that: described edible fungi is Pleurotus ostreatus.
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Cited By (2)

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CN106748231A (en) * 2016-12-30 2017-05-31 广西壮族自治区农业科学院微生物研究所 A kind of selenium enriched oyster mushroom culture medium and application method containing Moringa bits
CN106966769A (en) * 2017-02-14 2017-07-21 广西肽王生物科技有限公司 A kind of method that utilization Moringa branch bar prepares edible bacterium bag

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CN103460983A (en) * 2013-08-05 2013-12-25 滁州市南谯区大柳镇凤胜食用菌专业合作社 Cultivation method for liver-protection health-care ganoderma lucidum
CN104106370A (en) * 2014-06-04 2014-10-22 保山富群农业科技有限公司 Liquid strain of edible mushrooms and method for producing liquid strain of edible mushrooms

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Publication number Priority date Publication date Assignee Title
CN103460982A (en) * 2013-08-05 2013-12-25 滁州市南谯区大柳镇凤胜食用菌专业合作社 Culturing method of lucid ganoderma having effects of replenishing qi and protecting health
CN103460983A (en) * 2013-08-05 2013-12-25 滁州市南谯区大柳镇凤胜食用菌专业合作社 Cultivation method for liver-protection health-care ganoderma lucidum
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Publication number Priority date Publication date Assignee Title
CN106748231A (en) * 2016-12-30 2017-05-31 广西壮族自治区农业科学院微生物研究所 A kind of selenium enriched oyster mushroom culture medium and application method containing Moringa bits
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