CN106074210A - Cosmetic composition, application thereof and cosmetic essence - Google Patents

Cosmetic composition, application thereof and cosmetic essence Download PDF

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Publication number
CN106074210A
CN106074210A CN201610566011.5A CN201610566011A CN106074210A CN 106074210 A CN106074210 A CN 106074210A CN 201610566011 A CN201610566011 A CN 201610566011A CN 106074210 A CN106074210 A CN 106074210A
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China
Prior art keywords
skin
cosmetic composition
parts
weight
water
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Granted
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CN201610566011.5A
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Chinese (zh)
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CN106074210B (en
Inventor
戴跃锋
何广文
陈维
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Syoung Cosmetics Manufacturing Co Ltd
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Hunan Yujia Cosmetics Manufacturing Co ltd
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Priority to CN201610566011.5A priority Critical patent/CN106074210B/en
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/645Proteins of vegetable origin; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dermatology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Cosmetics (AREA)

Abstract

The invention relates to a cosmetic composition, application thereof and cosmetic essence. The active components of the cosmetic composition comprise, by weight, 410-60 parts of biological carbohydrate gum, 1-20 parts of inonotus obliquus plant peptide and 1-10 parts of oat polypeptide. The biogum glycocoll-4 forms a physical barrier film on the surface of the skin, so that dust or fine particles are prevented from being attached, and further, the generation of free radicals on the surface of the skin can be reduced; the inonotus obliquus plant peptide can be beneficial to controlling skin grease excessive secretion, regulating the balance of skin water and oil secretion, further reducing the attachment of fine particles on the skin and further reducing the generation of free radicals on the surface of the skin; the oat polypeptide can eliminate free radicals generated by dust, fine particulate matters and UV radiation of skin, and prevent collagen decomposition and natural antioxidant consumption in the skin. The active components have synergistic effect on the skin, and play a role in resisting exogenous environmental injury together through multiple protection on the skin.

Description

Cosmetic composition and application and cosmetics essence
Technical field
The present invention relates to household chemicals field, particularly relate to a kind of cosmetic composition and application thereof and cosmetics essence.
Background technology
Showing according to the study, exposing aerial skin is inevitably affected by from external environment, and environment Skin can be made injury by the dust of exogenous, fine particle or ultraviolet especially.The impact on skin of the most exogenous environment Or injury receives the extensive concern of researcher.
Exogenous environment shows that on the impact of skin or injury we live the every aspect of surrounding.
As routine work live in human body inevitably by various radiation, wherein UV (Ultraviolet Rays, Ultraviolet) radiation is fairly obvious to the injury of skin, and UV irradiates skin and produces free radical, and free radical accelerates collagen protein in skin Decomposition cause the problems such as skin histology atrophy scaly dry skin, wrinkle, mottle, corse sweat pore.
Additionally it is reported, the people in the whole world 80% lives in full fine particle, and (PM2.5, i.e. surrounding air hollow is pneumatic Mechanics equivalent diameter is less than or equal to the particulate matter of 2.5 microns) environment in the middle of, PM2.5 can cause skin to aoxidize according to the study Reduction reaction forms free radical, thus causes protein function in skin to be degenerated and Natural antioxidant consumption, and then affects flesh Skin tissue and the physiological function of cell.It is chronically exposed in the environment of full fine particle, easily causes the damage of skin, cause Skin produces wrinkle and pigmentation, causes the premature aging of skin;Skin is also easy to become responsive type reactivity skin, causes Skin produces series of problems.
And have at present resist the cosmetics of exogenous environmental injury function in cosmetic field be one relatively blank Field.Therefore, exploitation has and resists the cosmetics of exogenous environmental injury function and have higher market using value and weight The realistic meaning wanted.
Summary of the invention
Based on this, one of purpose of this invention is to provide a kind of cosmetics having and resisting exogenous environmental injury function Compositions.
The concrete technical scheme realizing foregoing invention purpose is as follows:
A kind of cosmetic composition, in parts by weight, its active component includes:
Biological carbohydrate gum-4 10~60 parts
Phaeoporus obliquus plant peptide 1~20 parts
Oat polypeptide 1~10 parts.
Above-mentioned cosmetic composition, biological carbohydrate gum-4 forms physics Obstruct membrane in skin surface, prevents dust or fine grained Thing adheres to, and then also can reduce the generation of skin surface free radical;Phaeoporus obliquus plant peptide can beneficially control skin oils and fats transition and divide Secrete, the balance of regulation skin water oil secretion, reduce fine particle attachment on skin further, and then also can reduce skin table The generation of face free radical;Oat polypeptide can eliminate the free radical that skin produces because of dust, fine particle and UV radiation, it is to avoid skin Middle collagen protein decomposes and Natural antioxidant consumption.Between each active constituent, skin is had Synergistic protective effect, By the multiple protective of skin being played jointly the effect resisting exogenous environmental injury.Additionally, above-mentioned cosmetic composition is also There is the skin moisturizing effect of excellence.
Additionally, biological carbohydrate gum-4 and Phaeoporus obliquus plant peptide reduce dust or fine particle at skin jointly by different mechanism On attachment, so increasing the amount of Phaeoporus obliquus plant peptide, the amount of biological carbohydrate gum-4 can be reduced within the specific limits.And it is biological sugared Glue-4 and Phaeoporus obliquus plant peptide advantageously reduce the generation of skin surface free radical, so increasing biological carbohydrate gum-4 and/or Betula platyphylla Suk. The amount of fine and soft plant, can reduce the amount of oat polypeptide within the specific limits.Then lacking of skin surface generation is eliminated by oat polypeptide Amount free radical.Above-mentioned cosmetic composition complements each other between each active constituent, coordination potentiation, multiple protective skin, real Now resist the function of exogenous environmental injury.
The pharmacology of contained active component is as follows:
Biological carbohydrate gum-4 (No. CAS: 283602-75-5), by biofermentation technique by plant fermentation.It has guarantor Wet reparation effect, can form one layer of ventilative sugar film simultaneously in skin, effectively stop the dust in air or fine particle etc. no Good pollutant, also have certain barriering effect simultaneously to ultraviolet.
Oat polypeptide: a kind of polypeptide obtained through enzymolysis by avenin.It is small active peptides, containing substantial amounts of parent Water base group, has the performance of keeping humidity of excellence, can form protective layer at skin surface by fast skin penetration;And can effectively block skin The great chain reaction of skin nocuity, eliminates free radical, accelerates cell proliferation and metabolism, Cucumber Extract, supplements skin glue Former albumen, strengthens skin elasticity.
Preferably, described cosmetic composition, in parts by weight, its active component includes:
Biological carbohydrate gum-4 30~60 parts
Phaeoporus obliquus plant peptide 5~10 parts
Oat polypeptide 6~10 parts.
Wherein in an embodiment, described cosmetic composition also include stabilizer, described stabilizer include polyhydric alcohol, Thickening agent and water, described stabilizer percentage by weight in described cosmetic composition is 20%~88%.Wherein thickening agent Play the solvent that Stabilization, polyhydric alcohol and water are thickening agent.Polyhydric alcohol not only has solvent action, also has antibacterial anticorrosion concurrently and makees With, improve cosmetic composition further and resist exogenous environmental injury function.
Wherein in an embodiment, the ratio of weight and number of described polyhydric alcohol, described thickening agent and described water is 20~60 Part: 0.1~1 part: 10~30 parts.Preferably, the ratio of weight and number of described polyhydric alcohol, described thickening agent and described water is 25~40 Part: 0.1~0.5 part: 15~30 parts.
Wherein in an embodiment, in parts by weight,
Described active component includes:
Biological carbohydrate gum-4 40 parts
Phaeoporus obliquus plant peptide 5 parts
Oat polypeptide 8 parts;
Described stabilizer includes:
Polyhydric alcohol 30 parts
Thickening agent 0.2 part
16.8 parts of water.
Wherein in an embodiment, described polyhydric alcohol selected from propylene glycol, butanediol, 1,2-pentanediol, 1,2-hexanediol, One or more in caprylyl glycol and Sensiva SC50.
Wherein in an embodiment, in parts by weight, described polyhydric alcohol includes 1,2-pentanediol and butanediol.Preferably , in parts by weight, described in described polyhydric alcohol 1, the ratio of weight and number of 2-pentanediol and described butanediol is 5 parts: 25 parts.
Wherein in an embodiment, described thickening agent one in carbomer, xanthan gum and hydroxyethyl cellulose Or it is multiple.
The preparation method of above-mentioned cosmetic composition, comprises the steps:
After thickening agent being disperseed and dissolves in polynary alcohol and water by proportioning, then by proportioning to its add biological carbohydrate gum-4, Phaeoporus obliquus plant peptide and oat polypeptide, stirring and evenly mixing.
Another object of the present invention is to provide the application of above-mentioned cosmetic composition.
Concrete technical scheme is as follows:
The application in preparing cosmetics of the above-mentioned cosmetic composition.
Another object of the present invention is to provide a kind of cosmetics essence having and resisting exogenous environmental injury function.
Concrete technical scheme is as follows:
A kind of cosmetics essence, its raw material includes substrate and above-mentioned cosmetic composition;Described substrate and described cosmetic Product compositions mixes, and obtains described cosmetics essence.
Above-mentioned cosmetics essence, can be used for face surface stuck type facial film, non-stimulated to skin gentleness, has no side effect, can have Effect resists the exogenous environmental injury such as dust, fine particle and UV radiation.
Wherein in an embodiment, described cosmetic composition percentage by weight in described raw material be 1%~ 10%.
Wherein in an embodiment, described raw material includes: 88.48% water, 2% butanediol, 3% glycerol, 0.05% saturating Bright matter acid sodium, 0.15% xanthan gum, 1% trehalose, 1% glycine betaine, 0.2% allantoin, 0.15% methyl hydroxybenzoate, 0.15% Cosmetic composition described in carbomer, 0.12% arginine, 0.2% phenoxyethanol, 0.5% spice and 3%.
Accompanying drawing explanation
Fig. 1 is that the skin moisture content that the experiment of embodiment 5 cosmetic composition performance of keeping humidity obtains is schemed over time;
Fig. 2 is before the water that embodiment 6 cosmetic composition prevents dust or fine particle from damaging performance test rinses The MIcrosope image of rear skin surface carbon dust distribution, wherein a, b are respectively skin surface carbon dust distribution before and after comparison water rinses MIcrosope image;C, d are respectively the MIcrosope image of skin surface carbon dust distribution before and after experimental group water rinses.
Detailed description of the invention
For the ease of understanding the present invention, below with reference to relevant drawings, the present invention is described more fully.In accompanying drawing Give the preferred embodiment of the present invention.But, the present invention can realize in many different forms, however it is not limited to herein Described embodiment.On the contrary, providing the purpose of these embodiments is to make the understanding to the disclosure more saturating Thorough comprehensively.
Below in conjunction with specific embodiment, cosmetic composition of the present invention and preparation method thereof is made further details of theory Bright.
Wherein, biological carbohydrate gum-4 is provided by France's element Lai Biya company (Solabia Group);Betula platyphylla Suk. plant peptide is by Korea S Dou Lai company (Durae Corporation) provides;Oat polypeptide is provided by Shanghai millet ground bio tech ltd.
Embodiment 1
The present embodiment one cosmetic composition, including following raw material:
The preparation method of above-mentioned cosmetic composition, comprises the steps:
By proportioning by xanthan gum 1, after 2-pentanediol, butanediol and water disperse and dissolves, then add to it by proportioning Biological carbohydrate gum-4, Phaeoporus obliquus plant peptide and oat polypeptide, stirring and evenly mixing.
Embodiment 2
The present embodiment one cosmetic composition, including following raw material:
The preparation method of above-mentioned cosmetic composition, comprises the steps:
After hydroxyethyl cellulose being disperseed and dissolves in Sensiva SC50 and water by proportioning, then add to it by proportioning Biological carbohydrate gum-4, Phaeoporus obliquus plant peptide and oat polypeptide, stirring and evenly mixing.
Embodiment 3
The present embodiment one cosmetic composition, including following raw material:
The preparation method of above-mentioned cosmetic composition, comprises the steps:
By proportioning, carbomer and xanthan gum are disperseed in 1,2-hexanediol, 1,2-pentanediol, caprylyl glycol and water and dissolve After, then add biological carbohydrate gum-4, Phaeoporus obliquus plant peptide and oat polypeptide, stirring and evenly mixing by proportioning to it.
Embodiment 4
The present embodiment one cosmetic composition, including following raw material:
The preparation method of above-mentioned cosmetic composition, comprises the steps:
By proportioning by carbomer 1, after 2-hexanediol, propylene glycol and water disperse and dissolves, then add to it by proportioning Biological carbohydrate gum-4, Phaeoporus obliquus plant peptide and oat polypeptide, stirring and evenly mixing.
Embodiment 5 cosmetic composition performance of keeping humidity is tested
Experimenter is tested by the cosmetic composition using embodiment 1.Specific experiment method is as follows:
Experimenter is in room temperature 25 DEG C, and relative humidity is the 30min that sits quietly in the environment of 50%.The change of experimental group Example 1 Cosmetic compositions is according to 2mg/cm2Standard is applied in experimenter's inner forearm, and the good application area of labelling.Separately take pure water by 2mg/ cm2Standard is applied in same experimenter's another one inner forearm relevant position, and application area on labelling, as blank Matched group.
Use Corneometer CM825 instrument that application area carries out skin moisture content mensuration, test in every 15 minutes Once, when testing, 5 points read in application area take its mean values, follow-on test 120 minutes every time.Corneometer Moisture content weight content percentage ratio in the tested skin histology of CM825 Instrument measuring, thus obtain skin moisture content such as Fig. 1.
It can be seen from figure 1 that within testing time 0~120 minutes, the water content of experimental group skin is both significantly higher than comparison Group;Especially before the testing time in 0~90 minute, the water content of experimental group skin is higher than matched group nearly 30%.Thus say Bright, cosmetic composition of the present invention has the skin moisture-keeping of excellence and moistens effect.And moisture content of skin can affect skin surface Water and the formation of oils and fats hybrid films, and this layer of hybrid films can play physical barrier membrane interaction, prevents dust or fine particle attachment, And then skin aging can be prevented.
Embodiment 6 cosmetic composition prevents dust or fine particle from damaging performance test
Choose 10 experimenters.Two forearm correspondence positions of the same experimenter are respectively as experimental group and matched group. The forearm of experimental group is coated with the cosmetic composition of embodiment 1, and the forearm of matched group is smeared and is left intact;Exist the most again The solution that equivalent contains carbon dust is smeared in forearm Experimental Area, then rinses skin surface carbon dust with water respectively, smears carbon dust and uses water Amount and the method rinsed are identical.The carbon dust distribution situation of skin surface before and after being rinsed by observation by microscope water.Choose Image before and after the two forearm use water flushings of one of them experimenter, as shown in Figure 2.
As can be seen from Figure 2, before no matter being flushing or after flushing, the carbon dust of experimental group skin surface attachment is all less than matched group The carbon dust of skin surface attachment.Thus illustrate that cosmetic composition of the present invention can effectively stop fine particle on skin attached , thus reduce the free radical that skin produces because of dust and fine particle, it is to avoid in skin, collagen protein decomposes and natural antioxygen Agent consumes.
Embodiment 7 cosmetics essence and preparation method thereof
A kind of cosmetics essence, its raw material composition and percentage by weight are as shown in table 1.
The raw material composition of table 1 cosmetics essence and percentage by weight
The preparation method of above-mentioned cosmetics essence, comprises the steps:
1, accurately weigh each component of A phase, add in reaction pot, heated and stirred to 80~85 DEG C, it is stirred until homogeneous and complete CL;
2, accurately weighing each component of B phase, mixing obtains B phase completely to dissolving, B phase puts into reaction pot and neutralizes, stirring After Jun Yun, stop heating and open cooling water temperature;
3, accurately weighing each component of C phase, mix homogeneously obtains C phase, when question response pot is cooled to 40~45 DEG C, by C phase Putting in reaction pot, stir, cooling obtains cosmetics essence.
It is appreciated that, it is possible to each raw material in table 1 is mixed, obtains cosmetics essence.
Described in the raw material of above-mentioned cosmetics essence, the percentage by weight of cosmetic composition is 3%.It can be used for face Surface stuck type facial film, non-stimulated to skin gentleness, have no side effect, can effectively resist the external sources such as dust, fine particle and UV radiation Property environmental injury.
Each technical characteristic of embodiment described above can combine arbitrarily, for making description succinct, not to above-mentioned reality The all possible combination of each technical characteristic executed in example is all described, but, as long as the combination of these technical characteristics is not deposited In contradiction, all it is considered to be the scope that this specification is recorded.
Embodiment described above only have expressed the several embodiments of the present invention, and it describes more concrete and detailed, but also Can not therefore be construed as limiting the scope of the patent.It should be pointed out that, come for those of ordinary skill in the art Saying, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement, these broadly fall into the protection of the present invention Scope.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.

Claims (11)

1. a cosmetic composition, it is characterised in that in parts by weight, its active component includes:
Biological carbohydrate gum-4 10~60 parts
Phaeoporus obliquus plant peptide 1~20 parts
Oat polypeptide 1~10 parts.
Cosmetic composition the most according to claim 1, it is characterised in that described cosmetic composition also includes stable Agent, described stabilizer includes polyhydric alcohol, thickening agent and water, described stabilizer percentage by weight in described cosmetic composition It is 20%~88%.
Cosmetic composition the most according to claim 2, it is characterised in that described polyhydric alcohol, described thickening agent and described The ratio of weight and number of water is 20~60 parts: 0.1~1 part: 10~30 parts.
Cosmetic composition the most according to claim 3, it is characterised in that in parts by weight,
Described active component includes:
Biological carbohydrate gum-4 40 parts
Phaeoporus obliquus plant peptide 5 parts
Oat polypeptide 8 parts;
Described stabilizer includes:
Polyhydric alcohol 30 parts
Thickening agent 0.2 part
16.8 parts of water.
5. according to the cosmetic composition described in any one of claim 2~4, it is characterised in that described polyhydric alcohol is selected from the third two One or more in alcohol, butanediol, 1,2-pentanediol, 1,2-hexanediol, caprylyl glycol and Sensiva SC50.
Cosmetic composition the most according to claim 5, it is characterised in that described polyhydric alcohol includes 1,2-pentanediol and fourth Glycol.
7. according to the cosmetic composition described in any one of claim 2~4, it is characterised in that described thickening agent is selected from card ripple One or more in nurse, xanthan gum and hydroxyethyl cellulose.
8. the application in preparing cosmetics of the cosmetic composition described in any one of claim 1~7.
9. a cosmetics essence, it is characterised in that its raw material includes the change described in substrate and any one of claim 1~7 Cosmetic compositions;Described substrate mixes with described cosmetic composition, obtains described cosmetics essence.
Cosmetics essence the most according to claim 9, it is characterised in that described cosmetic composition is at described raw material In percentage by weight be 1%~10%.
11. cosmetics essences according to claim 10, it is characterised in that described raw material includes: 88.48% water, 2% Butanediol, 3% glycerol, 0.05% hyaluronate sodium, 0.15% xanthan gum, 1% trehalose, 1% glycine betaine, 0.2% allantoin, Change described in 0.15% methyl hydroxybenzoate, 0.15% carbomer, 0.12% arginine, 0.2% phenoxyethanol, 0.5% spice and 3% Cosmetic compositions.
CN201610566011.5A 2016-07-18 2016-07-18 Cosmetic composition, application thereof and cosmetic essence Active CN106074210B (en)

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Cited By (9)

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CN106726893A (en) * 2016-12-06 2017-05-31 蔡日芬 A kind of Huanghua Pear Essence and preparation method thereof
CN107822946A (en) * 2017-10-10 2018-03-23 广州柏俐臣化妆品有限公司 A kind of painting type water light porcelain flesh Essence and preparation method thereof
CN109875922A (en) * 2019-04-01 2019-06-14 北京爸爸的选择科技有限公司 A kind of man Song Chunlu surfactant and preparation method thereof
CN110292538A (en) * 2019-07-25 2019-10-01 广州睿森生物科技有限公司 A kind of composition and its application in the anti-ageing cosmetics of preparation whitening
CN110522681A (en) * 2019-10-18 2019-12-03 广州芳利医药科技有限公司 A kind of anti-oxidant moisture retention water and its preparation method and application
CN111214427A (en) * 2020-02-29 2020-06-02 宁波法迪曼生物工程有限公司 Acid-free skin refreshing composition, preparation method thereof and essence containing composition
CN111557878A (en) * 2020-06-28 2020-08-21 无锡简玺生物科技有限公司 Essence containing human-like collagen and Cistus umbellatus extract and preparation method thereof
CN114504532A (en) * 2022-03-02 2022-05-17 广东巴松那生物科技有限公司 Composition with effects of resisting oxidation and relieving skin, preparation method and application
CN117243844A (en) * 2023-11-16 2023-12-19 成都普什制药有限公司 Collagen-containing skin care product essence and preparation method and application thereof

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106726893A (en) * 2016-12-06 2017-05-31 蔡日芬 A kind of Huanghua Pear Essence and preparation method thereof
CN107822946A (en) * 2017-10-10 2018-03-23 广州柏俐臣化妆品有限公司 A kind of painting type water light porcelain flesh Essence and preparation method thereof
CN109875922A (en) * 2019-04-01 2019-06-14 北京爸爸的选择科技有限公司 A kind of man Song Chunlu surfactant and preparation method thereof
CN110292538A (en) * 2019-07-25 2019-10-01 广州睿森生物科技有限公司 A kind of composition and its application in the anti-ageing cosmetics of preparation whitening
CN110522681A (en) * 2019-10-18 2019-12-03 广州芳利医药科技有限公司 A kind of anti-oxidant moisture retention water and its preparation method and application
CN111214427A (en) * 2020-02-29 2020-06-02 宁波法迪曼生物工程有限公司 Acid-free skin refreshing composition, preparation method thereof and essence containing composition
CN111557878A (en) * 2020-06-28 2020-08-21 无锡简玺生物科技有限公司 Essence containing human-like collagen and Cistus umbellatus extract and preparation method thereof
CN114504532A (en) * 2022-03-02 2022-05-17 广东巴松那生物科技有限公司 Composition with effects of resisting oxidation and relieving skin, preparation method and application
CN114504532B (en) * 2022-03-02 2024-02-23 广东巴松那生物科技有限公司 Composition with antioxidant and skin soothing effects, and preparation method and application thereof
CN117243844A (en) * 2023-11-16 2023-12-19 成都普什制药有限公司 Collagen-containing skin care product essence and preparation method and application thereof

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