CN106035215A - Pelophylax nigromaculata breeding method - Google Patents
Pelophylax nigromaculata breeding method Download PDFInfo
- Publication number
- CN106035215A CN106035215A CN201610424927.7A CN201610424927A CN106035215A CN 106035215 A CN106035215 A CN 106035215A CN 201610424927 A CN201610424927 A CN 201610424927A CN 106035215 A CN106035215 A CN 106035215A
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- China
- Prior art keywords
- black speck
- cylinder mould
- pleat frog
- frog
- cultural method
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
Abstract
A Pelophylax nigromaculata breeding method includes: putting natural enemies of tadpole in a breeding pond in a breeding process from oviposition of Pelophylax nigromaculata to successful tadpole metamorphosis, wherein the natural enemies are put in a net cage, and the net cage is arranged in water. In this way, the smell of the natural enemies can stimulate surrounding eggs of the Pelophylax nigromaculata and the tadpole, the anti-disease ability of the tadpole can be enhanced, the body weight and the body length of young Pelophylax nigromaculata metamorphosed by the tadpole can be increased, and the benefits and the yield of Pelophylax nigromaculata breeding can be increased.
Description
Technical field
The present invention relates to frog culture technical field, particularly relate to a kind of black speck side pleat frog cultural method.
Background technology
At present, in the breeding process of the black speck side pleat frog, raiser is in order to improve income, and raising of will trying every possible means is produced
Amount, therefore cultivates and first must clear up culturing pool, and kill the black speck side pleat frog and the natural enemy of Tinea Ranae and rival, kill the most exactly
Other biological in dead culturing pool, it is provided that the black speck side pleat frog is carried out the environment of single cultivation.Under such environment, the black speck side pleat frog
In developmental process, lack some viabilitys, be unfavorable for growing up healthy and sound of the black speck side pleat frog, the most liable to illness or weak, individual
Long is little.And prior art quickly increases the black speck side pleat frog generally by increase feedstuff and nourishing additive agent, this
Sample not only increases cost, and is unfavorable for the meat of the black speck side pleat frog.
Summary of the invention
The invention solves the problems that the problem that above-mentioned prior art exists, it is provided that a kind of black speck side pleat frog cultural method, by sky
Enemy stimulates the growth of black speck side pleat frog Tinea Ranae, not only increases the volume after the pleat frog metamorphosis of black speck side, and improves offspring and adapt to energy
Power and meat.
The present invention solve its technical problem use technical scheme: this black speck side pleat frog cultural method, at black speck side pleat
The frog lays eggs in the abnormal successfully breeding process of Tinea Ranae, puts into the natural enemy of Tinea Ranae in culturing pool, and natural enemy closes in cylinder mould, cylinder mould
It is placed in water.The so ovum of the Odor stimulation periphery black speck side pleat frog of natural enemy and Tinea Ranae, be possible not only to strengthen the existence of Tinea Ranae and
Premunition, after Tinea Ranae metamorphosis is black speck side pleat froglet, the volume of black speck side pleat froglet increases the most relatively, and body weight becomes weight, thus
Improve adaptive capacity to environment and the yield of the black speck side pleat frog.
In order to the most perfect, the preferred Procambarus clarkii of natural enemy or mosquito fish.Procambarus clarkii or mosquito fish are the ovum of the black speck side pleat frog
With the natural enemy of Tinea Ranae, put into the effect of stimulation produced in water preferable.
The most perfect, cylinder mould has several and is spaced 3-5 and puts a cylinder mould, 15 Procambarus clarkii of every cylinder mould.Due to sky
The abnormal smells from the patient of enemy is diffused with certain limit, and therefore interval 3-5 puts a cylinder mould, makes abnormal smells from the patient gamut be distributed.
The most perfect, the cylinder mould point of 6-9 by day and 18-21 point are put in the water of culturing pool.The black speck side pleat frog is in morning, evening
Movable more, increase the stimulation to it with two time periods of evening the most in the morning, strengthen the health of the black speck side pleat frog, be conducive to
Put on weight.
The most perfect, after the black speck side pleat frog lays eggs, in culturing pool, pour stimulation liquid into, specifically comprise the following steps that
The first step, preparation stimulates liquid: put into 50 Procambarus clarkii in 50 black speck side pleat frog Tinea Ranaes and cylinder mould in container,
After 12h, pull black speck side pleat frog Tinea Ranae and Procambarus clarkii out, solution filtered through gauze is obtained clear filtrate, i.e. stimulates liquid；
Second step, adds in culturing pool and stimulates liquid, and interval 12h adds once stimulates liquid.
The distribution of cylinder mould is difficult to visibly homogeneous, and therefore Tinea Ranae can use and stimulate liquid away from the cylinder mould equipped with natural enemy, stimulate liquid
Can be sprayed on the region that Tinea Ranae is intensive, the effect stimulating liquid is obvious, suitably sprays according to Tinea Ranae distribution.
The invention have the advantages that: the present invention uses the method for uniqueness to stimulate ovum and the Tinea Ranae of the black speck side pleat frog, not only
Strengthen the premunition of Tinea Ranae, increase the head of Tinea Ranae, after Tinea Ranae metamorphosis is the black speck side pleat frog, the volume also phase of the black speck side pleat frog
To increase, body weight becomes weight, thus improves the yield of the black speck side pleat frog, is conducive to being widely popularized.
Accompanying drawing explanation
The abnormal time that Fig. 1 is froglet, NP: without natural enemy, EP: have natural enemy；
Fig. 2 is that the body of froglet is long, NP: without natural enemy, EP: have natural enemy；
Fig. 3 is the body weight of froglet, NP: without natural enemy, EP: have natural enemy；
Fig. 4 is the skip capability of froglet, NP: without natural enemy, EP: have natural enemy.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described:
1 materials and methods
1.1 experiment equipment
Phjytotron (10M2), analytical balance (± 0.0001g, Sai Duolisi scientific instrument company limited), biochemical permanent
Temperature incubator, XTS3 type anatomical lens (Tyke, Beijing Instrument Ltd.), plant pulverizer (the Tianjin limited public affairs of Stettlen instrument
Department), digital camera (Sony DSC-T100), electronic digital indicator (± 0.01mm), HOBO record block and software, plastic box
(700L×500W×400H mm3), plastic bowl (bore 20mm), dredger, culture dish, Morris type water maze video tracking divide
Analysis system (Anhui Zhenghua Biology Instrument Equipment Co., Ltd.) etc..
1.2 laboratory animal collection and spawn incubations
At night, by pocket lamp irradiate from persistency culturing pool with dredger (mesh 2mm) manual capture nearly 15 big
Little similar Procambarus clarkii, takes back amphibians faunal laboratory, raises at outdoor tank-culture (3.0 × 2.0 × 1.5m3In) standby
With, hungry 24h before experiment.
From identical persistency culturing pool, gather number nest black speck side pleat Rana ovum group, take back amphibians faunal laboratory,
The square big plastic box (700L × 500W × 400H mm being momentarily placed in climate room (20.8 ± 0.2 DEG C)3, the 200mm depth of water) and standby
With.
1.3 experimentation
Take out 200 pieces at random from this nest black speck side pleat frog germ cell above-mentioned, be divided into 2 parts, be sequentially placed into square plastic hopper
(180×130×60mm3, the depth of water is 6cm) in, numbered 1 and 2, then carry out following 2 conditions process respectively: little without Predator
The blank of Lobster, put into Procambarus clarkii and process always.
Two forelimbs (42 phase) occur as abnormal mark using Tinea Ranae, start that to Tinea Ranae two forelimb afterbodys occur from experiment
It is wholly absent experienced natural law and is designated as period of metamorphosis.Weigh body weight, and measure froglet body with electronic digital indicator (± 0.01mm)
Long (distance from kiss end to urostyle).
Every black speck side pleat froglet, after indoor (25 ± 1 DEG C) endoadaptation 10-15min, puts it into the non-polluted edible of 1%
Pigment solution soaks its extremity, pulls out and be placed on the ground being covered with white antiskid paper, touch its tail gently with willow branch
Bar bone stimulates it to jump, and at least allows every froglet jump more than 10 times.Jump length is determined, from upper one by coating vestige
The end of individual hind leg trace is a jump to the distance between the end of next hind leg trace, measures the longest by three with ruler
Secondary skip distance (± 0.01cm), obtains meansigma methods.
2 results
Fig. 1 is shown in the statistically description of abnormal correlated characteristic.Single factor test multivariate analysis of variance (One-way MANOVA) is tied
Fruit shows, different Predators arrange the abnormal time of Tinea Ranae, body length and body weight, skip distance significant difference (Wilks λ=0.83,
Df=4,88, P < 0.002), Tukey ' s test shows, does not has the body length of froglet under conditions of Predator and body weight notable
The situation (allP < 0.004) existed less than Predator, the not notable (P of two kinds of abnormal times arranged and froglet skip distance difference
>0.10)。
It is apparent that different letters represents significant difference (a > b), NP: without sky from the icon of Fig. 1,2,3 and 4
Enemy, EP: have natural enemy, in the environment of having natural enemy, the abnormal time of the black speck side pleat frog, body length, body weight and skip capability have increase.
Although the present invention is by being described with reference to preferred embodiment, but, those skilled in the art should
Work as understanding, in the range of claims, the various change in form and details can be made.
Claims (5)
1. a black speck side pleat frog cultural method, is characterized in that: laying eggs to Tinea Ranae metamorphosis the black speck side pleat frog successfully cultivated
Cheng Zhong, puts into the natural enemy of Tinea Ranae in culturing pool, and natural enemy closes in cylinder mould, and cylinder mould is placed in water.
Black speck side the most according to claim 1 pleat frog cultural method, is characterized in that: described natural enemy is Procambarus clarkii or food mosquito
Fish.
Black speck side the most according to claim 1 pleat frog cultural method, is characterized in that: the every 3-5 of described cylinder mould puts a net
Cage, 15 Procambarus clarkii of every cylinder mould.
Black speck side the most according to claim 1 pleat frog cultural method, is characterized in that: the described cylinder mould point of 6-9 by day and 18-
In 21 water putting into culturing pool.
Black speck side the most according to claim 1 pleat frog cultural method, is characterized in that: after the black speck side pleat frog lays eggs, and is supporting
Pour stimulation liquid in growing water into, specifically comprise the following steps that
The first step, preparation stimulates liquid: put into 50 Procambarus clarkii in 50 black speck side pleat frog Tinea Ranaes and cylinder mould, 12h in container
After, pull black speck side pleat frog Tinea Ranae and Procambarus clarkii out, solution filtered through gauze is obtained clear filtrate, i.e. stimulates liquid；
Second step, adds in culturing pool and stimulates liquid, and sooner or later adding at interval of 12h once stimulates liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610424927.7A CN106035215B (en) | 2016-06-16 | 2016-06-16 | Blackspot side pleat frog cultural method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610424927.7A CN106035215B (en) | 2016-06-16 | 2016-06-16 | Blackspot side pleat frog cultural method |
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| CN106035215A true CN106035215A (en) | 2016-10-26 |
| CN106035215B CN106035215B (en) | 2017-06-23 |
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| CN201610424927.7A Active CN106035215B (en) | 2016-06-16 | 2016-06-16 | Blackspot side pleat frog cultural method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1251145A (en) * | 1997-03-25 | 2000-04-19 | 吉利斯德股份有限公司 | Chelate-forming fiber, process for preparing same, and use thereof |
| CN101543193A (en) * | 2008-03-28 | 2009-09-30 | 广东省昆虫研究所 | Method for breeding Chinese rana in Yuebei |
| CN104381213A (en) * | 2014-11-14 | 2015-03-04 | 劳炳凤 | High-density frog breeding method |
-
2016
- 2016-06-16 CN CN201610424927.7A patent/CN106035215B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1251145A (en) * | 1997-03-25 | 2000-04-19 | 吉利斯德股份有限公司 | Chelate-forming fiber, process for preparing same, and use thereof |
| CN101543193A (en) * | 2008-03-28 | 2009-09-30 | 广东省昆虫研究所 | Method for breeding Chinese rana in Yuebei |
| CN104381213A (en) * | 2014-11-14 | 2015-03-04 | 劳炳凤 | High-density frog breeding method |
Non-Patent Citations (1)
| Title |
|---|
| 阿舍小虎等: "龙虱对高原林蛙蝌蚪生长和存活率的影响", 《广东农业科学》 * |
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| CN106035215B (en) | 2017-06-23 |
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