CN105994999A - Method for preparing high-protein corn cob feed - Google Patents
Method for preparing high-protein corn cob feed Download PDFInfo
- Publication number
- CN105994999A CN105994999A CN201610411866.0A CN201610411866A CN105994999A CN 105994999 A CN105994999 A CN 105994999A CN 201610411866 A CN201610411866 A CN 201610411866A CN 105994999 A CN105994999 A CN 105994999A
- Authority
- CN
- China
- Prior art keywords
- preparation
- yeast
- quality protein
- described step
- liquid spawn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for preparing high-protein corn cob feed and belongs to the technical field of feed. The method includes the steps of preparation of a to-be-fermented matrix, preparation of a pleurotus ostreatus liquid strain, preparation of a yeast liquid strain and matrix fermentation. Corn cobs are fermented by means of pleurotus ostreatus (white rot fungi) and yeast, and in the process that the corn cobs are fermented by means of white rot fungi and yeast, the nutrition structure of the matrix is greatly changed. White rot fungi degrade lignin first, lignin is exposed, and accordingly palatability and the digestibility of straw feed are improved. White rot fungi can degrade cellulose and hemicellulose for bacterial growth, and degraded sugar can be used by yeast in time. Most part of low-quality non-protein nitrogen is converted into high-quality bacterial protein, and protein quality (mainly amino acid balance) is greatly improved. After the corn cobs are fermented by means of white rot fungi and yeast, nutrition value of the matrix is high, taste is good, and the feed has the flavor of mushrooms.
Description
Technical field
The invention belongs to field of feed, be specifically related to the preparation method of a kind of Quality Protein Maize core feedstuff.
Background technology
Semen Maydis is extremely wide at the distribution area of China, and annual production is up to hundred billion kilograms, occupies the 2nd, the world, its
Middle corn cob yield accounts for 10%, reaches more than ten million ton.Corn cob is the cob after corn ear goes seed threshing,
Typically constitute from corncob weight 20.0%-30.0%.Corn cob contains abundant cellulose (32%-36%), and half
Cellulose (35%-40%) and lignin (17%-20%).Lignin exists with a kind of physical barriers, surrounds fiber
Element, becomes peripheral stem matter, and protection cellulose exempts from the attack of enzyme.But become many and develop wooden group
One obstacle of the course of industrialization knitted.Effectively remove the lignin in corn cob, become the class of constantly research
Topic.
Summary of the invention
According to problems of the prior art, the invention provides the preparation of a kind of Quality Protein Maize core feedstuff
Method, can the effective lignin in degrading maize core, improve palatability and the digestion profit of straw feed
By rate.
In order to solve the problems referred to above, the present invention by the following technical solutions:
The preparation method of a kind of Quality Protein Maize core feedstuff, comprises the following steps:
Step one: corn cob dries dedusting, pulverized 30-40 mesh sieve standby；
Step 2: treat preparing after the corn cob of percentage by weight respectively 80%, the wheat bran mix homogeneously of 20%
Fermentation substrate；
Step 3: by weight percentage by 3% glucose, 0.2% peptone, 0.05%MgSO4·7H2O、
0.01%CaCl2, 0.05%KH2PO4And the water mix homogeneously of surplus, 121 DEG C of sterilizing 20min, after cooling, connect
Plant flat mushroom strain, prepare Pleurotus ostreatus liquid spawn；
Step 4: 100Inoculation yeast bacterium in P malt extract medium, prepares yeast liquid spawn；
Step 5: fermentation: will treat that fermentation substrate packs, in 121 DEG C, sterilizing 2 hours, after cooling, to connect
Kind of amount was 10% access Pleurotus ostreatus liquid spawn, and accesses yeast liquid spawn, in 26 DEG C of constant temperature culture 30 days
After, dry to constant weight, pulverize.
Preferably, after described step 3 inoculates flat mushroom strain, in 25 DEG C, 130r/min shaken cultivation 5
My god.
Preferably, in described step 4, yeast is saccharomyces cerevisiae, candida tropicalis, Candida utilis ferment
One or more in mother.
Preferably, in described step 5, the inoculum concentration of yeast liquid spawn is 5%-20%.
Preferably, described step 5 is to access 10% while 10% access Pleurotus ostreatus liquid spawn with inoculum concentration
Candida utilis liquid spawn.
Preferably, in described step 5, substrate water content to be fermented is 65%.
Preferably, in described step 5, pack uses polypropylene plastics pocket.
Preferably, described step one was pulverized 30 mesh sieves.
Preferably, described step 5 is treated the every sacked material of fermentation substrate 1000 grams.
The beneficial effects of the present invention is:
The present invention uses Pleurotus ostreatus (whiterot fungi) and yeast fermented maize core altogether, mixes at whiterot fungi and yeast
During bacterium fermented maize core, the trophic structure of its substrate has a very large change.First whiterot fungi drops
Solve lignin, make cellulose come out, improve palatability and the digestive utilization ratio of straw feed；In vain
Rotten bacterium also is able to degraded cellulose and hemicellulose etc. and carries out thalli growth, and the saccharide of degraded also can be in time by ferment
Female bacterium utilizes；On the other hand the non-protein nitrogen of major part low-quality is converted into the tropina that quality is higher, albumen
Matter quality (mainly amino acid balance) also obtains improvement by a relatively large margin.And corn cob is through Pleurotus ostreatus and yeast
After bacterium is fermented altogether, its substrate is not only of high nutritive value, in good taste, and has mushroom fragrance.
Detailed description of the invention
Embodiment 1
The preparation method of a kind of Quality Protein Maize core feedstuff, comprises the following steps:
Step one: corn cob dries dedusting, pulverized 30 mesh sieves standby；
Step 2: treat preparing after the corn cob of percentage by weight respectively 80%, the wheat bran mix homogeneously of 20%
Fermentation substrate；
Step 3: by weight percentage by 3% glucose, 0.2% peptone, 0.05%MgSO4·7H2O、
0.01%CaCl2, 0.05%KH2PO4And the water mix homogeneously of surplus, 121 DEG C of sterilizing 20min, after cooling, connect
Plant flat mushroom strain, prepare Pleurotus ostreatus liquid spawn；
Step 4: 100P malt extract medium is inoculated saccharomyces cerevisiae, prepares saccharomyces cerevisiae liquid spawn；
Step 5: fermentation: by moisture content be 65% treat fermentation substrate load polypropylene plastics pocket, every sacked material
1000 grams, in 121 DEG C, sterilizing 2 hours, after cooling, is 10% access Pleurotus ostreatus liquid spawn with inoculum concentration,
After 26 DEG C of constant temperature culture 20 days, then access 20% saccharomyces cerevisiae liquid spawn, in 26 DEG C of constant temperature culture
10 days, dry to constant weight, pulverize.
Embodiment 2
The preparation method of a kind of Quality Protein Maize core feedstuff, comprises the following steps:
Step one: corn cob dries dedusting, pulverized 30 mesh sieves standby；
Step 2: treat preparing after the corn cob of percentage by weight respectively 80%, the wheat bran mix homogeneously of 20%
Fermentation substrate；
Step 3: by weight percentage by 3% glucose, 0.2% peptone, 0.05%MgSO4·7H2O、
0.01%CaCl2, 0.05%KH2PO4And the water mix homogeneously of surplus, 121 DEG C of sterilizing 20min, after cooling, connect
Plant flat mushroom strain, prepare Pleurotus ostreatus liquid spawn；
Step 4: 100P malt extract medium is inoculated candida tropicalis, prepares candida tropicalis liquid
Body strain；
Step 5: fermentation: by moisture content be 65% treat fermentation substrate load polypropylene plastics pocket, every sacked material
1000 grams, in 121 DEG C, sterilizing 2 hours, after cooling, is that 10% access Pleurotus ostreatus liquid spawn is same with inoculum concentration
Time access 20% candida tropicalis liquid spawn, after 26 DEG C of constant temperature culture 30 days, dry to constant weight,
Pulverize.
Embodiment 3
The preparation method of a kind of Quality Protein Maize core feedstuff, comprises the following steps:
Step one: corn cob dries dedusting, pulverized 30 mesh sieves standby；
Step 2: treat preparing after the corn cob of percentage by weight respectively 80%, the wheat bran mix homogeneously of 20%
Fermentation substrate；
Step 3: by weight percentage by 3% glucose, 0.2% peptone, 0.05%MgSO4·7H2O、
0.01%CaCl2, 0.05%KH2PO4And the water mix homogeneously of surplus, 121 DEG C of sterilizing 20min, after cooling, connect
Plant flat mushroom strain, prepare Pleurotus ostreatus liquid spawn；
Step 4: 100P malt extract medium is inoculated Candida utilis, prepares Candida utilis liquid
Body strain；
Step 5: fermentation: by moisture content be 65% treat fermentation substrate load polypropylene plastics pocket, every sacked material
1000 grams, in 121 DEG C, sterilizing 2 hours, after cooling, is that 10% access Pleurotus ostreatus liquid spawn is same with inoculum concentration
Time access 10% Candida utilis liquid spawn, after 26 DEG C of constant temperature culture 30 days, dry to constant weight,
Pulverize.
In the Quality Protein Maize core feedstuff that embodiment 3 prepares, lignin and cellulose have dropped 53.1% He respectively
39.3%.
Protein content in the Quality Protein Maize core feedstuff that embodiment 1-3 prepares see table 1.
Table 1
| Embodiment | Protein content (%) before fermentation | Protein content (%) after fermentation |
| Embodiment 1 | 5.344 | 11.98±0.63 |
| Embodiment 2 | 5.344 | 12.00±0.21 |
| Embodiment 3 | 5.344 | 13.25±0.36 |
Claims (9)
1. the preparation method of a Quality Protein Maize core feedstuff, it is characterised in that comprise the following steps:
Step one: corn cob dries dedusting, pulverized 30-40 mesh sieve standby；
Step 2: treat preparing after the corn cob of percentage by weight respectively 80%, the wheat bran mix homogeneously of 20%
Fermentation substrate；
Step 3: by weight percentage by 3% glucose, 0.2% peptone, 0.05%MgSO4·7H2O、
0.01%CaCl2, 0.05%KH2PO4And the water mix homogeneously of surplus, 121 DEG C of sterilizing 20min, after cooling, connect
Plant flat mushroom strain, prepare Pleurotus ostreatus liquid spawn；
Step 4: 100Inoculation yeast bacterium in P malt extract medium, prepares yeast liquid spawn；
Step 5: fermentation: will treat that fermentation substrate packs, in 121 DEG C, sterilizing 2 hours, after cooling, to connect
Kind of amount was 10% access Pleurotus ostreatus liquid spawn, and accesses yeast liquid spawn, in 26 DEG C of constant temperature culture 30 days
After, dry to constant weight, pulverize.
2. according to the preparation method of a kind of Quality Protein Maize core feedstuff shown in claim 1, it is characterised in that:
Described step 3 is inoculated after flat mushroom strain, in 25 DEG C, 130r/min shaken cultivation 5 days.
3. according to the preparation method of a kind of Quality Protein Maize core feedstuff shown in claim 1, it is characterised in that:
One during yeast is saccharomyces cerevisiae, candida tropicalis, Candida utilis in described step 4 or
Several.
4. according to the preparation method of a kind of Quality Protein Maize core feedstuff shown in claim 1, it is characterised in that:
In described step 5, the inoculum concentration of yeast liquid spawn is 5%-20%.
5. according to the preparation method of a kind of Quality Protein Maize core feedstuff shown in claim 1, it is characterised in that:
In described step 5 with inoculum concentration be access while 10% access Pleurotus ostreatus liquid spawn 10% Candida utilis ferment
Female liquid spawn.
6. according to the preparation method of a kind of Quality Protein Maize core feedstuff shown in claim 1, it is characterised in that:
In described step 5, substrate water content to be fermented is 65%.
7. according to the preparation method of a kind of Quality Protein Maize core feedstuff shown in claim 1, it is characterised in that:
In described step 5, pack uses polypropylene plastics pocket.
8. according to the preparation method of a kind of Quality Protein Maize core feedstuff shown in claim 1, it is characterised in that:
Described step one was pulverized 30 mesh sieves.
9. according to the preparation method of a kind of Quality Protein Maize core feedstuff shown in claim 1, it is characterised in that:
Described step 5 is treated the every sacked material of fermentation substrate 1000 grams.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610411866.0A CN105994999A (en) | 2016-06-14 | 2016-06-14 | Method for preparing high-protein corn cob feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610411866.0A CN105994999A (en) | 2016-06-14 | 2016-06-14 | Method for preparing high-protein corn cob feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105994999A true CN105994999A (en) | 2016-10-12 |
Family
ID=57091074
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610411866.0A Pending CN105994999A (en) | 2016-06-14 | 2016-06-14 | Method for preparing high-protein corn cob feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105994999A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107095008A (en) * | 2017-04-05 | 2017-08-29 | 新疆农业职业技术学院 | A kind of Quality Protein Maize core feed and preparation method thereof |
| CN107173527A (en) * | 2017-07-13 | 2017-09-19 | 安徽师范大学 | A kind of preparation method of the straw feed of high crude protein content |
| CN107683956A (en) * | 2017-10-18 | 2018-02-13 | 沈阳金博地生态环保科技有限公司 | It is a kind of using maize peel and stalk waste residue as feed of raw material and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102232466A (en) * | 2010-05-04 | 2011-11-09 | 洋浦慷民高科生物有限公司 | Bio-protein feed prepared by adopting cellulose raw material and preparation method thereof |
| CN102246890A (en) * | 2010-05-20 | 2011-11-23 | 盐城工学院 | Making method for producing biologic protein feeds by using corncobs |
| CN103082145A (en) * | 2013-01-29 | 2013-05-08 | 大连工业大学 | Method for producing grape skin residue pig feed by utilizing lentinula edodes and yeast for symbiotic fermentation |
| CN105475623A (en) * | 2016-01-20 | 2016-04-13 | 宾石其 | Production method and feeding method of ecological fungal compound feed |
-
2016
- 2016-06-14 CN CN201610411866.0A patent/CN105994999A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102232466A (en) * | 2010-05-04 | 2011-11-09 | 洋浦慷民高科生物有限公司 | Bio-protein feed prepared by adopting cellulose raw material and preparation method thereof |
| CN102246890A (en) * | 2010-05-20 | 2011-11-23 | 盐城工学院 | Making method for producing biologic protein feeds by using corncobs |
| CN103082145A (en) * | 2013-01-29 | 2013-05-08 | 大连工业大学 | Method for producing grape skin residue pig feed by utilizing lentinula edodes and yeast for symbiotic fermentation |
| CN105475623A (en) * | 2016-01-20 | 2016-04-13 | 宾石其 | Production method and feeding method of ecological fungal compound feed |
Non-Patent Citations (1)
| Title |
|---|
| 柯丽霞等: "白腐菌混菌发酵玉米芯木质素酶系分泌的种属互惠性研究", 《食品与发酵工业》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107095008A (en) * | 2017-04-05 | 2017-08-29 | 新疆农业职业技术学院 | A kind of Quality Protein Maize core feed and preparation method thereof |
| CN107173527A (en) * | 2017-07-13 | 2017-09-19 | 安徽师范大学 | A kind of preparation method of the straw feed of high crude protein content |
| CN107683956A (en) * | 2017-10-18 | 2018-02-13 | 沈阳金博地生态环保科技有限公司 | It is a kind of using maize peel and stalk waste residue as feed of raw material and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1313017C (en) | Zymophyte of plant straw feedstuff and its preparation | |
| CN108041289B (en) | Silage additive special for paper mulberry and application thereof | |
| CN103168921A (en) | Method for producing straw feed | |
| CN106396807A (en) | Culture medium for improving bioconversion rate of edible fungi, preparation method of culture medium and cultivation method of edible fungi | |
| CN102246890A (en) | Making method for producing biologic protein feeds by using corncobs | |
| CN103564199A (en) | Method for producing cow feed by multi-strain mixing solid-state fermentation | |
| CN104012787A (en) | Method for preparing corn straw coarse feed by microbial beneficial living bacteria | |
| CN109527200A (en) | A kind of Pleurotus eryngii germ bran biological fermented feed and preparation method | |
| CN108142660A (en) | A kind of preparation method of straw feed | |
| CN104855680A (en) | Pleurotus nebrodensis fungus chaff fermented feed and processing technology thereof | |
| CN101361520B (en) | Potato pulp energy fermentation feed capable of replacing bran and preparation method thereof | |
| CN105475623A (en) | Production method and feeding method of ecological fungal compound feed | |
| CN110384177A (en) | It is a kind of to prepare the method for Yeast culture and its application in animal feed based on distillers ' grains | |
| CN106721195A (en) | A kind of ruminant concentrated feed and preparation method thereof | |
| CN113796455A (en) | Process for producing feed by using crop straws | |
| CN109845899A (en) | A kind of sugarcane end pin white wine residue protein feed and preparation method thereof | |
| CN108378200A (en) | A kind of earthworm feedstuff base and preparation method thereof | |
| CN111165653A (en) | Potato residue and corn straw mixture fermented feed and preparation method and leavening agent thereof | |
| CN104472869A (en) | Mushroom bran protein biological feed additive and preparation method thereof | |
| CN105994999A (en) | Method for preparing high-protein corn cob feed | |
| CN106804875A (en) | A kind of sweet potato residue fermented feed and preparation method and application | |
| CN103229888B (en) | Method for preparing protein feedstuff through straw solid-state fermentation by using neurospora crassa | |
| CN108813109A (en) | A kind of composite fungi fermented stalk feed and preparation method thereof | |
| CN108260713A (en) | The method that germ bran biological fermented feed is made using vinegar grain | |
| CN112335783A (en) | Mixed silage of sweet sorghum and lemon leaves and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161012 |