CN105994368B - A kind of medicament and its spraying method for promoting radix pseudostellariae growth and root tuber to grow - Google Patents
A kind of medicament and its spraying method for promoting radix pseudostellariae growth and root tuber to grow Download PDFInfo
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- CN105994368B CN105994368B CN201610445450.0A CN201610445450A CN105994368B CN 105994368 B CN105994368 B CN 105994368B CN 201610445450 A CN201610445450 A CN 201610445450A CN 105994368 B CN105994368 B CN 105994368B
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- radix pseudostellariae
- root tuber
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
Abstract
The invention discloses medicaments and its spraying method that a kind of promotion radix pseudostellariae growth and root tuber are grown, belong to field of plant growing technology.It sprays medicinal plant radix pseudostellariae nursery stock blade face using trace element solution, continuously sprays twice at the beginning of mid-April and 5 months.The present invention can promote the nutrient growth of nursery stock aerial part, also help the growth and development of root tuber, and single radix pseudostellariae root tuber quality and yield per unit area is made to be significantly improved, further, it is also possible to improve radix pseudostellariae photosynthetic capacity.The microelement has many advantages, such as that easy using concentration and at low cost, high-efficiency low-toxicity, processing method, application technology is mature, has good application value to the high-yield culturing of medicinal plant radix pseudostellariae.
Description
Technical field
The invention belongs to field of plant growing technology, strong more specifically to a kind of growth of promotion radix pseudostellariae and root tuber
Big medicament and its spraying method.
Background technique
Radix pseudostellariae (Radix Pseudostellariae) is the dried root of pinkwort pseudostellaria heterophylla, is a kind of
The traditional tonic medicine material of dual-purpose of drug and food is widely used in treatment diabetes, coronary heart diseases and angina pectoris, leukopenia, first by medical field
The difficult and complicated cases such as high, scrofula.In recent years, polysaccharide, saponin, cyclic peptide, microelement etc. are isolated from radix pseudostellariae root tuber
Multiple types active constituent, these active constituents are the bases of radix pseudostellariae quality.Exempt from the study found that Pseudostellaria Polysaccharide has to improve
A variety of effects such as the content, function of physical fatigue alleviation of hemolysin and inhibition growth of tumour cell in epidemic disease serum.Radix pseudostellariae soap
Glucoside has treatment palpitaition, antioxidant activity and antivirus action, stronger to blister blight cytotoxic activity inhibiting effect.Radix pseudostellariae cyclic peptides pair
Cruel oxygen acid enzyme and B16 cell have strong inhibitory activity, can treat common chromatodermatosis such as sparrow
Spot, chloasma, senile plaque.Radix pseudostellariae can be not only used for treatment disease, be also used to develop health care product and cosmetics, application prospect
It is relatively wide.In recent years, the problems such as radix pseudostellariae drug requirement is gradually increased, and generally existing provenance is degenerated in addition, and disease is serious makes
Radix pseudostellariae Severe Reduction, quality and quantity are difficult to ensure, it has also become the bottleneck of radix pseudostellariae exploitation.Therefore the height of radix pseudostellariae is solved
It is most important to produce main points of cultivation.
Summary of the invention
1, it to solve the problems, such as
For the above-mentioned problems in the prior art, the present invention provide it is a kind of promotion radix pseudostellariae growth and root tuber grow
Medicament and its spraying method can promote the nutrient growth of nursery stock aerial part, also help the growth and development of root tuber, make list
A radix pseudostellariae root tuber quality and yield per unit area are significantly improved.
2, technical solution
To solve the above-mentioned problems, the technical solution adopted in the present invention is as follows:
A kind of spraying method for the medicament for promoting radix pseudostellariae growth and root tuber to grow, chooses and grows normal medicinal plant too
Son ginseng, radix pseudostellariae root tuber expanding stage is twice in succession in medicinal plant radix pseudostellariae foliage spray medicament at the beginning of mid-April and 5 months;Institute
The time interval for stating the medicament described in medicinal plant radix pseudostellariae foliage spray twice in succession is 20-24 days, and the medicament is dense
Degree is the rare earth salt aqueous solution of 100mg/L.
Further, the time interval of the medicament described in medicinal plant radix pseudostellariae foliage spray twice in succession is 3 weeks.
Further, each fountain height of the medicament is 500ml/m2。
A kind of medicament for promoting radix pseudostellariae growth and root tuber to grow, the medicament are the rare earth salt water that concentration is 100mg/L
Solution.
Further, the rare earth salt aqueous solution is lanthanum nitrate hexahydrate.
3, beneficial effect
Compared with the prior art, the invention has the benefit that
(1) present invention can not only be such that radix pseudostellariae nursery stock radix pseudostellariae plants by the Rare Earth Lanthanum solution by using suitable concentration
Young plant height, ground diameter, height of seedling and ground diameter relative growth yield, root tuber length, root tuber diameter, root tuber be fresh, dry weight, yield per unit area etc. refer to
Mark dramatically increases, so as to remarkably promote the growth of medicinal plant radix pseudostellariae, improve single root tuber quality, yield per unit area.No
It only can be improved medicinal plant radix pseudostellariae photosynthetic capacity and beet yield, and the microelement has and uses concentration and cost
The advantages that low, high-efficiency low-toxicity, processing method are easy, application technology is mature, there is well the high-yield culturing of medicinal plant radix pseudostellariae
Application value;
(2) the Rare Earth Lanthanum solution of the invention by spraying suitable quality concentration, can not only promote nursery stock aerial part
Nutrient growth also helps the growth and development of root tuber, obtains single radix pseudostellariae root tuber quality and yield per unit area significantly
It improves, and optimum concentration is 100mg/L;
(3) present invention, which sprays suitable concentration Rare Earth Lanthanum (25-400mg/L) in root tuber expanding stage, can promote radix pseudostellariae overground part
Growth, show as that height improves and ground diameter increases, wherein 100mg/L concentration treatment effect is best, and height of seedling and ground diameter are relatively raw
Long amount increases by 45.45%, 33.33% than control group respectively, significant (p ﹤ 0.05) with contrast difference.Show the nitre of debita spissitudo
Sour lanthanum handles while promoting radix pseudostellariae seedling-height growth and thickening to grow;
(4) present invention can promote the development of radix pseudostellariae root tuber by spraying the Rare Earth Lanthanum solution of suitable quality concentration, wherein
100mg/L concentration treatment effect is best, which keeps radix pseudostellariae main root length, root tuber length, root tuber diameter, root tuber fresh and dry weight difference
7.40%, 48.04%, 13.97%, 18.84%, 16.21% is increased than control group, significant difference (p ﹤ 0.05).Show to fit
When the lanthanum nitrate of concentration can effectively facilitate the development of radix pseudostellariae root tuber and biomass accumulation.And concentration processing makes radix pseudostellariae unit
Area output is fresh, dry weight increases 58.34%, 56.87% than control group respectively, significant difference (p ﹤ 0.05).
(5) present invention can make radix pseudostellariae nursery stock radix pseudostellariae Plant Leaf by spraying the Rare Earth Lanthanum solution of suitable quality concentration
Chlorophyll contents, carotenoid content and Net Photosynthetic Rate dramatically increase, and show that the lanthanum nitrate processing of debita spissitudo can be shown
It writes and improves radix pseudostellariae net photosynthetic rate;Specifically, radix pseudostellariae blade net photosynthetic rate nitric acid la concn be 100mg/L when,
Net Photosynthetic Rate annual average is maximum, increases by 43.44% than control, significant difference (p < 0.05);
Detailed description of the invention
Fig. 1 is nitric acid in a kind of medicament and its spraying method for promoting radix pseudostellariae growth and root tuber to grow of the present invention
Influence schematic diagram of the lanthanum to radix pseudostellariae height of seedling dynamic change;
Fig. 2 is nitric acid in a kind of medicament and its spraying method for promoting radix pseudostellariae growth and root tuber to grow of the present invention
Influence schematic diagram of the lanthanum to radix pseudostellariae ground diameter dynamic change;
Fig. 3 is nitric acid in a kind of medicament and its spraying method for promoting radix pseudostellariae growth and root tuber to grow of the present invention
Influence schematic diagram of the lanthanum to radix pseudostellariae height of seedling and ground diameter relative growth yield;
Fig. 4 is nitric acid in a kind of medicament and its spraying method for promoting radix pseudostellariae growth and root tuber to grow of the present invention
Influence schematic diagram of the lanthanum to radix pseudostellariae yield;
Fig. 5 is different in a kind of medicament and its spraying method for promoting radix pseudostellariae growth and root tuber to grow of the present invention
Influence schematic diagram of the concentration lanthanum nitrate to radix pseudostellariae Net Photosynthetic Rate Zhou Bianhua;
Fig. 6 is different in a kind of medicament and its spraying method for promoting radix pseudostellariae growth and root tuber to grow of the present invention
Influence schematic diagram of the concentration lanthanum nitrate to radix pseudostellariae Net Photosynthetic Rate diurnal variation.
Fig. 7 is different in a kind of medicament and its spraying method for promoting radix pseudostellariae growth and root tuber to grow of the present invention
Influence schematic diagram of the concentration lanthanum nitrate to radix pseudostellariae chlorophyll content.
Fig. 8 is different in a kind of medicament and its spraying method for promoting radix pseudostellariae growth and root tuber to grow of the present invention
Influence schematic diagram of the concentration lanthanum nitrate to radix pseudostellariae carotenoid content.
Specific embodiment
The present invention is further described below combined with specific embodiments below.
A kind of medicament for promoting radix pseudostellariae growth and root tuber to grow, which is characterized in that the medicament is that concentration is 25-
The Rare Earth Lanthanum of 700mg/L.
A kind of spraying method for the medicament for promoting radix pseudostellariae growth and root tuber to grow, which is characterized in that it is normal to choose growth
Medicinal plant radix pseudostellariae, radix pseudostellariae root tuber expanding stage is twice in succession on medicinal plant radix pseudostellariae blade face at the beginning of mid-April and 5 months
Spray the medicament.The time interval of the medicament described in medicinal plant radix pseudostellariae foliage spray twice in succession is 20-24 days.
The each fountain height of the medicament is 500ml/m2。
Preferably, the time interval of the medicament described in medicinal plant radix pseudostellariae foliage spray twice in succession is preferably
3 weeks.
Embodiment
One, test material and experimental field overview
It is provided for examination radix pseudostellariae seedling by Fujian Province Zherong County, when test chooses robust growth, the consistent radix pseudostellariae of growing way
Seedling cultivation.Experimental field it is located at another name for Sichuan Province practice forest farm, 31 ° 59 ' of north latitude, 119 ° of east longitude under Nanjing Forestry University and belongs to north subtropical monsoon
Weather, 15.2 DEG C of average annual temperature, average annual rainfall be 1104mm, annual sunshine time 2018 hours, the annual average frost-free period 229
It.Soil is yellowish soil, and soil property attaches most importance to earth to loamy texture.Topsoil (0-20cm) soil contains full N 0.704gkg-1, crack N
78.63g·kg-1, full P 0.146gkg-1, effective P 1.295gkg-1, active potassic 39.7gkg-1, organic matter 7.39g
kg-1, pH 4.5-5.5.
Two, field trial is handled
The radix pseudostellariae nursery stock that test selection is grown and physiological status is more consistent, is arranged 15 experimental plots, plot area
2m2(2m×1m).Rare earth treatment use lanthanum nitrate hexahydrate foliage-spray method, concentration be respectively 0mg/L (CK), 25mg/L,
100mg/L, 400mg/L and 700mg/L), coprocessing 2 times, the time is respectively in mid-April, 2015 and the first tenday period of a month in May (radix pseudostellariae block
Root expanding stage), it is dripped with blade face for degree, each concentration processing is repeated 3 times.The normal field management such as moisture during test.
Three, testing index and method
(1) overground part growth indexes: height of seedling and ground diameter in the 4-6 month each processing randomly select 6 plants it is for statistical analysis,
It surveys 6 times altogether, every minor tick 7d.Height of seedling is measured using Stanley (Stanley) 30-6165m tape measure, and ground diameter is using 0~300MM electricity
Sub- vernier caliper measurement.
Height of seedling relative growth yield calculation formula are as follows: RGIH=(H1﹣ H0)/H0
Wherein: H0: measurement height of seedling height (cm) for the first time
H1Last time measurement height of seedling height (cm)
Ground stem relative growth yield calculation formula are as follows: RGID=(D1﹣ D0)/D0
Wherein: D0: measurement ground diameter width (mm) for the first time
D1Last time measurement ground diameter width (mm)
(2) root tuber growth indexes: single plant main root length, root tuber length, root tuber diameter, root tuber be fresh, dry weight and yield per unit area
Fresh, dry weight is measured radix pseudostellariae root tuber finished product after harvest.Main root is long and root tuber is long using Stanley (Stanley) 30-
The measurement of 6165m tape measure, root tuber diameter are measured using 0~300MM electronic vernier caliper, and fresh weight is to remove surface water after plant is cleaned
It is measured after point using electronic balance, dry weight is to be measured after drying using electronic balance.
(3) Net Photosynthetic Rate: in the 4-6 month using Li-6400 portable photosynthetic instrument measurement radix pseudostellariae plant Net Photosynthetic Rate week
Variation randomly selects 6 plants of for statistical analysis, survey 6 times altogether, every minor tick 7d in each processing.Minute is morning 9:00-
10:30, measurement day are fine.Net Photosynthetic Rate diurnal variation is measured May, each interval time of measurement is 2 hours.
(4) it pigment content: takes the fresh radix pseudostellariae blade 0.5g shredded to be put into mortar, adds a small amount of quartz sand and 95% second
Alcohol, is ground into homogenate, then plus ethyl alcohol continues to be ground to tissue turned pale a little, be filled into 25mL volumetric flask, constant volume.With 95% second
Alcohol compares, and optical density is measured at wavelength 665nm, 649nm and 470nm, as follows calculate chlorophyll a, chlorophyll b and
Carotenoid content:
Ca=13.95D665-6.88D649;
Cb=24.96D649-7.32D665;
CX·C=(1000 × D470-3.27×Ca-104×Cb)/229。
In formula: D665, D649And D470It is light of the chloroplast pigment extracting solution at wavelength 665nm, 649nm and 470nm respectively
Density.
(5) beet yield: fresh weight is to be measured after removing surface moisture after plant is cleaned using electronic balance, and dry weight is drying
It is measured afterwards using electronic balance.
(6) data processing
Data analysis is handled using origin and SPSS17.0 analysis software.
Four, analysis of experimental results
(1) influence of the microelement to radix pseudostellariae height of seedling, ground diameter and height of seedling ground diameter relative growth yield: as shown in Figure 1, Figure 2 and Fig. 3
Shown, the growth of radix pseudostellariae overground part can be promoted by spraying suitable concentration Rare Earth Lanthanum (25-400mg/L) in root tuber expanding stage, performance
It is improved for height and ground diameter increases, best with 100mg/L la concn facilitation effect, height of seedling and ground diameter relative growth yield compare respectively
According to increasing by 45.45%, 33.33%, significantly (p ﹤ 0.05) with contrast difference.Show the lanthanum nitrate processing of debita spissitudo while promoting
It is grown into radix pseudostellariae seedling-height growth and thickening.
(2) influence of the microelement to radix pseudostellariae root tuber growth indexes: as shown in table 1, Rare Earth Lanthanum is to radix pseudostellariae plant master
Root long, root tuber length, root tuber diameter, root tuber are fresh and dry weight has good facilitation, and are in apparent concentration effect, with 100mg/
L la concn facilitation effect is best.Concentration processing keeps radix pseudostellariae main root length, root tuber length, root tuber diameter, root tuber fresh and dry weight difference
7.40%, 48.04%, 13.97%, 18.84%, 16.21% is increased than CK, significant difference (p ﹤ 0.05).Show appropriate dense
The lanthanum nitrate of degree can effectively facilitate the development of radix pseudostellariae root tuber and biomass accumulation.
Influence of the 1 various concentration lanthanum nitrate of table to radix pseudostellariae plant strain growth index
(3) influence of the microelement to radix pseudostellariae beet yield: as shown in figure 4, compared with CK group, Rare Earth Lanthanum can be significant
Increase radix pseudostellariae yield per unit area, wherein 100mg/L processing group effect of increasing production effect is best, and concentration processing makes radix pseudostellariae list
Position area output is fresh, dry weight increases 58.34%, 56.87% than CK respectively, significant difference (p ﹤ 0.05).
(4) influence of the microelement to radix pseudostellariae Net Photosynthetic Rate Zhou Bianhua: as shown in figure 5, in various concentration lanthanum nitrate
Under the conditions of, radix pseudostellariae Net Photosynthetic Rate drops afterwards as the increase of nitric acid la concn has the tendency that first increasing, wherein with 100mg/L concentration
Lanthanum nitrate treatment effect it is best.Show that the lanthanum nitrate processing of debita spissitudo can significantly improve radix pseudostellariae blade net photosynthesis speed
Rate.
(5) influence of the microelement to radix pseudostellariae Net Photosynthetic Rate diurnal variation: as shown in fig. 6, respectively processing radix pseudostellariae blade
The diurnal variation curve of Net Photosynthetic Rate is in bimodal pattern, and the 1st peak value is above the 2nd peak value.1st peak value appears in 9:
00 or so, the Net Photosynthetic Rate that respectively handles later continues to decline, and low ebb value occurs to 11:00-13:00 or so, then net photosynthesis
Rate is gone up again, until there is the 2nd peak value in 15:00 again, decline rapidly again later.The day of radix pseudostellariae blade net photosynthetic rate
Variation has apparent " lunch break " phenomenon.When nitric acid la concn is 100mg/L, Net Photosynthetic Rate annual average is maximum, increases than control
43.44%, significant difference (p < 0.05).And the concentration can effectively alleviate " lunch break " phenomenon.
(6) influence of the microelement to radix pseudostellariae Net Photosynthetic Rate Chlorophylls and Carotenoids content: such as Fig. 7 and Fig. 8
Shown, during test, compared with the control, the lanthanum nitrate processing of debita spissitudo can improve chlorophyll and class recklessly to some extent
Radish cellulose content, with the increase of concentration for the treatment of, the trend fallen after rising generally is presented in the variation of pigment content, is had apparent
Concentration effect, best with 100mg/L concentration treatment effect, content is significantly higher than control (p ﹤ 0.05).High concentration processing simultaneously
(100mg/L) promotes effect even lower than CK.As it can be seen that the lanthanum nitrate processing of debita spissitudo can effectively adjust radix pseudostellariae blade
Pigment content promotes light energy absorption, but excessive concentration then plays inhibiting effect.
Based on above-mentioned, the present invention can not only make radix pseudostellariae nursery stock radix pseudostellariae by the Rare Earth Lanthanum by using suitable concentration
Plant height of seedling, ground diameter, height of seedling and ground diameter relative growth yield, root tuber length, root tuber diameter, root tuber be fresh, dry weight, yield per unit area etc.
Index dramatically increases, so as to remarkably promote the growth of medicinal plant radix pseudostellariae, improve single root tuber quality, yield per unit area,
And the microelement has many advantages, such as that easy using concentration and at low cost, high-efficiency low-toxicity, processing method, application technology is mature,
There is good application value to the high-yield culturing of medicinal plant radix pseudostellariae;It is molten by the Rare Earth Lanthanum for spraying suitable quality concentration
Liquid can not only significantly improve radix pseudostellariae root tuber quality and yield per unit area, can also improve radix pseudostellariae Net Photosynthetic Rate and
Leaf pigment concentration.
Schematically the present invention and embodiments thereof are described above, description is not limiting.So if
Those of ordinary skill in the art are inspired by it, without departing from the spirit of the invention, not inventively designs
Frame mode embodiment similar with the technical solution out, is within the scope of protection of the invention.
Claims (3)
1. a kind of spraying method for the medicament for promoting radix pseudostellariae growth and root tuber to grow, which is characterized in that it is normal to choose growth
Medicinal plant radix pseudostellariae, radix pseudostellariae root tuber expanding stage sprays on medicinal plant radix pseudostellariae blade face twice in succession at the beginning of mid-April and 5 months
Spill medicament;The time interval of the medicament described in medicinal plant radix pseudostellariae foliage spray twice in succession is 20-24 days, and described
Medicament is the rare earth salt aqueous solution that concentration is 100mg/L.
2. a kind of spraying method of medicament for promoting radix pseudostellariae growth and root tuber to grow according to claim 1, feature
It is, the time interval of the medicament described in medicinal plant radix pseudostellariae foliage spray twice in succession is 3 weeks.
3. a kind of spraying method of medicament for promoting radix pseudostellariae growth and root tuber to grow according to claim 1, feature
It is, each fountain height of the medicament is 500ml/m2。
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