CN105993956B - A kind of Atractylis lancea rapid propagation method - Google Patents
A kind of Atractylis lancea rapid propagation method Download PDFInfo
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- CN105993956B CN105993956B CN201610415933.6A CN201610415933A CN105993956B CN 105993956 B CN105993956 B CN 105993956B CN 201610415933 A CN201610415933 A CN 201610415933A CN 105993956 B CN105993956 B CN 105993956B
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
The present invention discloses a kind of Atractylis lancea rapid propagation method, includes the following steps：The selection and processing of seed：The big full Atractylis lancea seed of grain is chosen, is sterilized using the potassium permanganate of concentration 5 ~ 10%, using being impregnated 20 ~ 30 minutes in 75% alcohol after 30 minutes, is impregnated 5 ~ 10 minutes with 3% ammonium hydroxide, then clean using aseptic water washing；Seedling is cultivated：Atractylis lancea seed is seeded in cultivate 1 ~ 2 week on germination culture medium and grows seedling；The induction of Multiple Buds：The plumular axis of seedling is cut into culture in access differential medium and obtains Multiple Buds, Multiple Buds are divided into single plant continuously cultivated for 3 generations on differential medium and obtain regeneration plant；The induction of root：Transplantation of Regenerated Plantlets is obtained into the plant of well developed root system to root media root induction；Transplanting：Field management will be carried out in the plantlet of transplant to experimental plot of well developed root system and grow under field conditions (factors).What the present invention selected is that explant is bred, and the processing method enhances the survival rate of Atractylis lancea seedling.
Description
Technical field
The invention belongs to plant planting fields, and in particular to a kind of Atractylis lancea rapid propagation method.
Background technology
Atractylis lancea is common Chinese medicine, has drying damp and strengthening spleen, expelling wind and clearing away cold, improving eyesight and other effects, is a kind of medicinal plant in imminent danger
Object, according to investigations, rhizoma atractylodis currently on the market are mostly Atractylis chinensis, and the Atractylis lancea of real Jiangsu production is seldom seen, therefore in order to
The quick breeding for solving Atractylis lancea, has there is many propagation methods at present, but effect is not obvious, and there is also very big for survival rate
Problem.
Invention content
Goal of the invention：The object of the present invention is to provide a kind of Atractylis lancea rapid propagation methods, and this method bud ratio is high, training
The foster time is short, and operability is strong, and application value is high, less investment, and output is high.
Technical solution：To solve the above-mentioned problems, the present invention provides a kind of Atractylis lancea rapid propagation method, including it is following
Step：
1) selection and processing of seed：Choose the big full Atractylis lancea seed of grain, use mass concentration for 5~10% height
Potassium manganate sterilizes, use after 30 minutes volumetric concentration for 75% alcohol in impregnate 20~30 minutes, be 3% with mass concentration
Ammonium hydroxide impregnates 5~10 minutes, then clean using aseptic water washing；
2) seedling is cultivated：The Atractylis lancea seed of step 1) processing is seeded in cultivate 1~2 week on germination culture medium and grows children
Seedling；
3) induction of Multiple Buds：The plumular axis of seedling is cut into culture in access differential medium and obtains Multiple Buds, will be grown thickly
Bud, which is divided into single plant, continuously to be cultivated for 3 generations on differential medium and obtains regeneration plant；
4) induction of root：Root induction in Transplantation of Regenerated Plantlets to root media is obtained into the plant of well developed root system；
5) it transplants：Field management will be carried out in the plantlet of transplant to experimental plot of well developed root system and grow under field conditions (factors).
Wherein, above-mentioned steps 2) in germination culture medium be added in common MS culture mediums mass fraction be 0.01~
0.05% methyl α-naphthyl acetate.
Wherein, the cultivation temperature in step 2) is 28 DEG C, light intensity 1500LX, daily illumination 6 hours.
Wherein, the differential medium in step 3) is heteroauxin+0.05~0.15mg/L's of MS+0.5~1.5mg/L
2,4- dichlorphenoxyacetic acids, the medium pH are 6.5.
Wherein, the cultivation temperature in step 3) is 25 DEG C, light intensity 1800LX, and daily illumination culture in 8 hours obtains regeneration and plants
Strain.
Wherein, the root media in step 3) is 1/3MS+IBA0.02mg/L.
Wherein, above-mentioned steps 5) in the field management in experimental plot carry out weeding in 5~June, and monthly increase fertilizer one
It is secondary, the fertilizer be 50% rape cake, 20% calcium phosphate and 30% cow dung, the above percentage is weight percent.
Wherein, above-mentioned steps 5) in field management further include the prevention and control of plant diseases, pest control, be by by 60% phoxim baythion missible oil 660
Times liquid, which pours to be filled on planting site, carries out disease control；It is sprayed after being diluted according to operation instruction with bacillus thuringiensis suspending agent,
Prevention for insect pest.
Advantageous effect：The present invention compared with the existing technology, has the following advantages：
1) what the present invention selected is that explant is bred, and the mode handled is simple and environmentally-friendly, does not use toxic examination
Agent carries out disinfection processing, not damaged to Atractylis lancea bud, and the survival rate of processing method enhancing Atractylis lancea seedling.
2) the different stages that method of the invention uses use different culture mediums, obtain higher survival rate.
3) present invention has found out different condition of each stage by plantation, is finally obtained excellent Atractylis lancea product
Kind.
Specific implementation mode
The present invention is further described below.
In the present invention, bacillus thuringiensis suspending agent is bought from Jiangsu Nong He agricultural science and technologys Co., Ltd.Remaining chemicals
It is commercial product.
Embodiment 1
A kind of Atractylis lancea rapid propagation method, includes the following steps：
1) selection and processing of seed：Choose the big full Atractylis lancea seed of grain, use mass concentration for 10% Gao Meng
The disinfection of sour potassium, use after 30 minutes volumetric concentration for 75% alcohol in impregnate 30 minutes, be the immersion of 3% ammonium hydroxide with mass concentration
10 minutes, then it is clean using aseptic water washing；
2) seedling is cultivated：The Atractylis lancea seed of step 1) processing is seeded in cultivate 1~2 week on germination culture medium and grows children
Seedling；
3) induction of Multiple Buds：The plumular axis of seedling is cut into culture in access differential medium and obtains Multiple Buds, will be grown thickly
Bud, which is divided into single plant, continuously to be cultivated for 3 generations on differential medium and obtains regeneration plant；
4) induction of root：Root induction in Transplantation of Regenerated Plantlets to root media is obtained into the plant of well developed root system；
5) it transplants：Field management will be carried out in the plantlet of transplant to experimental plot of well developed root system and grow under field conditions (factors).
Germination culture medium is that the methyl α-naphthyl acetate that mass fraction is 0.05% is added in common MS culture mediums in step 2).
Cultivation temperature in step 2) is 28 DEG C, light intensity 1500LX, daily illumination 6 hours.
2, the 4- dichlorphenoxyacetic acids for the heteroauxin+0.15mg/L that differentiation culture in step 3) is MS+1.5mg/L,
The medium pH is 6.5.
Cultivation temperature in step 3) is 25 DEG C, light intensity 1800LX, and daily illumination culture in 8 hours obtains regeneration plant.
Root media in step 4) is 1/3MS+IBA0.02mg/L.
The field management in experimental plot carries out weeding in 5~June in step 5), and it is primary monthly to increase fertilizer, the fertilizer
Material be 50% rape cake, 20% calcium phosphate and 30% cow dung, the above percentage is weight percent.
Field management further includes the prevention and control of plant diseases, pest control in step 5), is by pouring 60% 660 times of liquid of phoxim baythion missible oil
On planting site.It is sprayed after being diluted according to operation instruction with bacillus thuringiensis suspending agent, is used for the prevention of insect pest.
The transplanting survival rate of the Atractylis lancea planted by the above method, Atractylis lancea seedling is 96%.
Embodiment 2
A kind of Atractylis lancea rapid propagation method, includes the following steps：
1) selection and processing of seed：Choose the big full Atractylis lancea seed of grain, use mass concentration for 5% permanganic acid
Potassium sterilize, use after 30 minutes volumetric concentration for 75% alcohol in impregnate 20 minutes, with mass concentration be 3% ammonium hydroxide immersion 5
Minute, then it is clean using aseptic water washing；
2) seedling is cultivated：The Atractylis lancea seed of step 1) processing is seeded in cultivate 1~2 week on germination culture medium and grows children
Seedling；
3) induction of Multiple Buds：The plumular axis of seedling is cut into culture in access differential medium and obtains Multiple Buds, will be grown thickly
Bud, which is divided into single plant, continuously to be cultivated for 3 generations on differential medium and obtains regeneration plant；
4) induction of root：Root induction in Transplantation of Regenerated Plantlets to root media is obtained into the plant of well developed root system；
5) it transplants：Field management will be carried out in the plantlet of transplant to experimental plot of well developed root system and grow under field conditions (factors).
Germination culture medium is that the methyl α-naphthyl acetate that mass fraction is 0.01% is added in common MS culture mediums in step 2).
Cultivation temperature in step 2) is 28 DEG C, light intensity 1500LX, daily illumination 6 hours.
2, the 4- dichlorphenoxyacetic acids for the heteroauxin+0.05mg/L that differentiation culture in step 3) is MS+0.5mg/L,
The medium pH is 6.5.
Cultivation temperature in step 3) is 25 DEG C, light intensity 1800LX, and daily illumination culture in 8 hours obtains regeneration plant.
Root media in step 4) is 1/3MS+IBA0.02mg/L.
The field management in experimental plot carries out weeding in 5~June in step 4), and it is primary monthly to increase fertilizer, the fertilizer
Material be 50% rape cake, 20% calcium phosphate and 30% cow dung, the above percentage is weight percent.
Field management further includes the prevention and control of plant diseases, pest control in step 5), is by pouring 60% 660 times of liquid of phoxim baythion missible oil
On planting site.It is sprayed after being diluted according to operation instruction with bacillus thuringiensis suspending agent, is used for the prevention of insect pest.
The transplanting survival rate of the Atractylis lancea planted by the above method, Atractylis lancea seedling is 93%.
Embodiment 3
A kind of Atractylis lancea rapid propagation method, includes the following steps：
1) selection and processing of seed：Choose the big full Atractylis lancea seed of grain, use mass concentration for 7% permanganic acid
Potassium sterilize, use after 30 minutes volumetric concentration for 75% alcohol in impregnate 25 minutes, with mass concentration for 3% ammonium hydroxide immersion
7 minutes, then it is clean using aseptic water washing；
2) seedling is cultivated：The Atractylis lancea seed of step 1) processing is seeded in cultivate 1~2 week on germination culture medium and grows children
Seedling；
3) induction of Multiple Buds：The plumular axis of seedling is cut into culture in access differential medium and obtains Multiple Buds, will be grown thickly
Bud, which is divided into single plant, continuously to be cultivated for 3 generations on differential medium and obtains regeneration plant；
4) induction of root：Root induction in Transplantation of Regenerated Plantlets to root media is obtained into the plant of well developed root system；
5) it transplants：Field management will be carried out in the plantlet of transplant to experimental plot of well developed root system and grow under field conditions (factors).
Germination culture medium is that the methyl α-naphthyl acetate that mass fraction is 0.03% is added in common MS culture mediums in step 2).
Cultivation temperature in step 2) is 28 DEG C, light intensity 1500LX, daily illumination 6 hours.
2, the 4- dichlorphenoxyacetic acids for the heteroauxin+0.1mg/L that differentiation culture in step 3) is MS+1mg/L, it is described
Medium pH is 6.5.
Cultivation temperature in step 3) is 25 DEG C, light intensity 1800LX, and daily illumination culture in 8 hours obtains regeneration plant.
Root media in step 4) is 1/3MS+IBA0.02mg/L.
The field management in experimental plot carries out weeding in 5~June in step 5), and it is primary monthly to increase fertilizer, the fertilizer
Material be 50% rape cake, 20% calcium phosphate and 30% cow dung, the above percentage is weight percent.
Field management further includes the prevention and control of plant diseases, pest control in step 5), is by pouring 60% 660 times of liquid of phoxim baythion missible oil
On planting site.It is sprayed after being diluted according to operation instruction with bacillus thuringiensis suspending agent, is used for the prevention of insect pest.
The transplanting survival rate of the Atractylis lancea planted by the above method, Atractylis lancea seedling is 95%.
The above is only a preferred embodiment of the present invention, it should be pointed out that：For the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (1)
1. a kind of Atractylis lancea rapid propagation method, which is characterized in that include the following steps：
1) selection and processing of seed：Choose the big full Atractylis lancea seed of grain, use mass concentration for 5 ~ 10% potassium permanganate
Disinfection, use after 30 minutes volumetric concentration for 75% alcohol in impregnate 20 ~ 30 minutes, with mass concentration for 3% ammonium hydroxide immersion 5
~ 10 minutes, then it is clean using aseptic water washing；
2) seedling is cultivated：The Atractylis lancea seed of step 1) processing is seeded in cultivate 1 ~ 2 week on germination culture medium and grows seedling；
3) induction of Multiple Buds：The plumular axis of seedling is cut into culture in access differential medium and obtains Multiple Buds, by Multiple Buds point
Continuously cultivating on differential medium for 3 generations at single plant obtains regeneration plant；
4) induction of root：By in Transplantation of Regenerated Plantlets to root media, root induction obtains the plant of well developed root system；
5) it transplants：Field management will be carried out in the plantlet of transplant to experimental plot of well developed root system and grow under field conditions (factors)；
Germination culture medium is that the naphthalene second that mass fraction is 0.01 ~ 0.05% is added in common MS culture mediums in the step 2)
Acid；Cultivation temperature in step 2) is 28 DEG C, light intensity 1500LX, daily illumination 6 hours；In step 3)
Differential medium is 2, the 4- dichlorphenoxyacetic acids of heteroauxin+0.05 ~ 0.15mg/L of MS+0.5 ~ 1.5mg/L, institute
It is 6.5 to state medium pH；Step 3）In cultivation temperature be 25 DEG C, light intensity 1800LX, the culture of daily illumination 8 hours obtains again
Raw plant, step 4）In root media be 1/3MS+IBA0.02mg/L, the step 5）The field management in middle experimental plot exists
5 ~ June carries out weeding, and monthly increases that fertilizer is primary, the fertilizer be 50% rape cake, 20% calcium phosphate and 30% cow dung,
The step 5）Middle field management further includes the prevention and control of plant diseases, pest control, is to be filled in plantation by pouring 60% 660 times of liquid of phoxim baythion missible oil
Disease control is carried out on the ground；It is sprayed after being diluted according to operation instruction with bacillus thuringiensis suspending agent, for the anti-of insect pest
It controls.
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| CN108617505A (en) * | 2017-03-24 | 2018-10-09 | 江苏省中国科学院植物研究所 | One kind inducing rapid propagation method of sprouting by RHIZOMA ATRACTYLODIS IANCEAE plumular axis |
| CN107439085A (en) * | 2017-07-28 | 2017-12-08 | 镇江绿健农业科技开发有限公司 | A kind of pre-sowing treatment method of Atractylis lancea seed |
| CN110352853B (en) * | 2019-08-26 | 2020-06-09 | 南京农业大学 | Method for improving quality and transplanting survival rate of atractylis lancea test-tube plantlet |
| CN115281085A (en) * | 2022-07-08 | 2022-11-04 | 内蒙古民族大学 | Tissue culture propagation method of rhizoma atractylodis macrocephalae |
| CN115362937B (en) * | 2022-08-26 | 2023-04-07 | 中国中医科学院中药研究所 | Rhizoma atractylodis test-tube plantlet and culture method thereof, and method for transplanting rhizoma atractylodis test-tube plantlet |
| CN115316274B (en) * | 2022-08-29 | 2023-03-14 | 中国中医科学院中药研究所 | Starting culture medium, rhizoma atractylodis lanceae test-tube plantlet and cultivation method thereof |
| CN115316278A (en) * | 2022-09-13 | 2022-11-11 | 湖北诗经源中药科技有限公司 | Seed propagation technology of atractylodes lancea of Compositae |
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| CN1307867C (en) * | 2005-02-25 | 2007-04-04 | 江苏大学 | Method for preserving germplasm for Atractylis lancea tissue culture propagation |
| CN103348913B (en) * | 2013-06-28 | 2015-04-15 | 江苏茅山地道中药材种植有限公司 | Rapid propagation method for bletilla striata |
| CN104012209B (en) * | 2014-06-26 | 2015-07-01 | 甘肃中医学院 | Method for promoting seeds of meconopsis integrifolia to germinate |
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