CN105974066A - Vertical type animal gastrointestinal tract bionic digestion device - Google Patents
Vertical type animal gastrointestinal tract bionic digestion device Download PDFInfo
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- CN105974066A CN105974066A CN201610423920.3A CN201610423920A CN105974066A CN 105974066 A CN105974066 A CN 105974066A CN 201610423920 A CN201610423920 A CN 201610423920A CN 105974066 A CN105974066 A CN 105974066A
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Abstract
A vertical type animal gastrointestinal tract bionic digestion device comprises a simulation digestive tract, a stirring rod, a motor and a magnetic transmission device. The simulation digestive tract comprises a vertical glass tube body and a dialysis tube body which is placed inside the vertical glass tube body. The stirring rod is installed in the dialysis tube body, and the top end of the stirring rod is detachably connected with the magnetic transmission device. The magnetic transmission device is installed above a silica gel plug at the top end of the simulation digestive tract and electrically connected with the motor. The motor is installed above the magnetic transmission device through a motor fixing base. The vertical type animal gastrointestinal tract bionic digestion device is simple in structure, convenient to use, good in accuracy and repeatability of digestion simulation and small in error, and bionic digestion reaction liquid leakage can be avoided.
Description
Technical field
The present invention relates to a kind of Bionic digestion experiment equipment, especially relate to a kind of vertical animal gastrointestinal tract Bionic digestion device.
Background technology
In modern farming, the objective nutrient biological value accurately evaluating feedstuff determines that Animal nutrition requirement and optimizes the primary decision-making basis of feed formula.From the sixties in 20th century, people are attempting realizing the rapid evaluation to feed nutrient biological value by the digestion in animal gastrointestinal tract of the conditions in vitro Imitating feedstuff always.
Prior art generally use triangular flask or test tube as gastrointestinal tract Bionic digestion device.When being simulated digestion experiment, first the feedstuff of constant weight is loaded in triangular flask or test tube, then in triangular flask or test tube, add simulated gastric fluid or intestinal juice, after again, triangular flask or test tube being placed on water bath with thermostatic control shaking table vibration digestion a period of time, this process is simulation animal stomach or the digestion process of intestinal, after digestion terminates, centrifuged or filtered again goes out oneself digestion material and undigested material, when the weight of undigested material is higher relative to the weight of Feed Sample, represents that digestibility is low.When using triangular flask or test tube as gastrointestinal tract Bionic digestion device, owing to the digestion product in triangular pipe or test tube has inhibitory action to digestion reaction, the digestive efficiency causing this analoglike digestion experiment is generally low than the measured value in animal body, affects the accurate test and appraisal to feed nutrient biological value.
Compared with triangular flask or test tube as Simulative digestive device for gastrointestinal tract of animals, the eighties in 20th century Canada scholar Gauther etc., Savoie etc. establishes with pig small intestine simulation digestive appartus as ingredient of Dialysis tubing (aperture 1000Da ~ 14000Da is optional), flowing dialysis solution system, agitator, water bath with thermostatic control system.
In addition, CN 201344874Y discloses a kind of Simulative digestive device for gastrointestinal tract of animals, it includes glass tubing and Dialysis tubing, this glass tubing is a hollow tube, this body be respectively arranged at the two ends with a ground, the side of this body is provided with input pipe and outlet tube, this Dialysis tubing is placed in this glass tubing, the two ends of this Dialysis tubing are stretched out at the two of this glass tubing grounds respectively and are turned up, the Dialysis tubing end being exposed to outside ground of turning up is tied up by rubber bar and is fixed on ground, ties up behind Dialysis tubing end two grounds and is plugged with a rubber stopper and respectively with the rubber stopper of tube for transfusion.
CN
104020264 A disclose a kind of gastrointestinal tract Bionic digestion device, including a support, multiple simulation digestive appartus, a Multi-hole-site test tube clamp, the plurality of simulation digestive appartus is clamped in described Multi-hole-site test tube clamp, and described Multi-hole-site test tube clamp fixedly mounts on the bracket；Each described simulation digestive appartus is provided with buffer input port, buffer delivery outlet and Digestive system input port；In two adjacent simulation digestive appartus, the buffer input port of a forward simulation digestive appartus is connected by infusion tube with the buffer delivery outlet of another simulation digestive appartus, and all of simulation digestive appartus is sequentially connected in series by described infusion tube.
The most several employing Dialysis tubings are as the device of animal gastrointestinal tract Bionic digestion, but this Dialysis tubing be horizontal be placed in air bath shaking table in or shaking bath in simulate digestion process, easily because of in Dialysis tubing feedstuff in digestion process aerogenesis and cause the rubber stopper of Bionic digestion tube end outwards to come off, the problem such as reactant liquor leakage, secondly, during Bionic digestion, treating that sample digestion easily sticks causes bag filter blocked on bag filter, and then affects the digestion effect of Feed Sample.
Summary of the invention
The technical problem to be solved in the present invention is: overcome the deficiencies in the prior art, it is provided that a kind of simple in construction, easy to use, can avoid the vertical animal gastrointestinal tract Bionic digestion device that Bionic digestion reactant liquor is revealed.
The technical solution adopted for the present invention to solve the technical problems is: a kind of vertical animal gastrointestinal tract Bionic digestion device, including simulation digestive tube, stirring rod, motor and magnetic drive device；Described simulation digestive tube includes vertical glass tubing and Dialysis tubing, and Dialysis tubing is placed in this vertical glass tubing；Described stirring rod is arranged in Dialysis tubing, and its top removably connects with magnetic drive device；Described magnetic drive device is arranged on above simulation digestive tube, electrically connects with motor；Described motor is arranged on above magnetic drive device by motor fixing seat.
Further, described vertical glass tubing is a hollow tube, and described hollow tube upper and lower side is respectively provided with a ground, and its side bottom and upper end are respectively equipped with input pipe and outlet tube；The two ends of Dialysis tubing are stretched out at the two of this vertical glass tubing grounds respectively and are turned up, the Dialysis tubing end being exposed to outside ground of turning up is tied up by rubber bar and is fixed on ground, ground after hollow tube upper and lower side ties up Dialysis tubing end is plugged with at least tube for transfusion (preferably respectively, the quantity of tube for transfusion is 2 ~ 6) silica gel plug and solid silicon plug, offer in the middle part of the described silica gel plug with at least tube for transfusion place magnetic drive device groove.
Further, described magnetic drive device includes that seat fixed by magnetic core, magnetic core；Described magnetic core is fixed and is provided with magnetic loop cover outside seat；Described magnetic core is fixed seat by bearing with magnetic core and is connected；The bottom of described magnetic core is connected with groove matched in clearance.
Further, described vertical animal gastrointestinal tract Bionic digestion device also includes the first sealing ring being fixed on magnetic drive device with the junction of the silica gel plug with tube for transfusion.
Further, described stirring rod is connected with magnetic drive device by connecting shaft, and the upper end of described connecting shaft is set in outside magnetic core, its bottom and the hollow top clamping of stirring rod.Preferably, described connecting shaft is silica gel tube.
Further, described magnetic drive device includes outer magnetic ring set, outer magnetic ring, isolation cover, internal magnetic ring and internal magnetic ring pitman；Described outer magnetic ring set is fixed with motor output shaft, upper seal bootr and is connected；Described outer magnetic ring is nested and is located in outer magnetic ring；It is provided with isolation cover between described internal magnetic ring and outer magnetic ring；Described internal magnetic ring is arranged in isolation cover；One end of described internal magnetic ring pitman snaps in internal magnetic ring, and its other end is inserted through lower seal bootr and silica gel plug, is connected with the top of stirring rod.
Further, described motor being arranged with motor cover, the bottom of described motor cover is connected with motor fixing seat, and described motor fixing seat is made up of upper seal bootr and lower seal bootr, is provided with the second sealing ring, is bolted to connection between upper seal bootr and lower seal bootr.
Further, described vertical animal gastrointestinal tract Bionic digestion device also includes the pumping peristaltic pump being connected with input pipe and the pumping peristaltic pump being connected with outlet tube.
Operation principle and the using method of a kind of vertical animal gastrointestinal tract Bionic digestion device of the present invention be: Feed Sample loads Dialysis tubing, and stomach simulated digestive juice is injected in Dialysis tubing by tube for transfusion.Then, there is the stomach buffer of a design temperature, at the input pipe with simulation digestive tube, under the effect pumping peristaltic pump and pumping peristaltic pump that outlet tube connects, inject in vertical glass tubing via input pipe, then flow out vertical glass tubing via outlet tube, and the power of pumping peristaltic pump is less than the power of pumping peristaltic pump, make between vertical glass tubing and the Dialysis tubing of simulation digestive tube, to form relatively low negative pressure (such as-0.01MPa ~-0.04MPa), and the liquid level of reactant liquor does not produce fluctuation in bag filter, make that the sample to be digested in bag filter is non-sticky to be adhered on Dialysis tubing tube wall, reduce the error of simulation digestion process.Like this, the stomach buffer of certain volume is such vertical glass tubing of inflow and outflow circularly within the setting time, meanwhile, the magnetic core of driven by motor magnetic drive device is fixed seat relative to magnetic core and is done cutting magnetic line movement, and then magnetic core rotates, and drive stirring rod to rotate by connecting shaft, or, driven by motor outer magnetic ring set and outer magnetic ring rotate, outer magnetic ring is made to produce rotating excitation field in isolation cover, drive internal magnetic ring rotates, snapping in the internal magnetic ring pitman in internal magnetic ring drives stirring rod to rotate, and then realize the stirring of the Feed Sample in bag filter with stomach simulated digestive juice is mixed, rotating speed >=the 100r/min of stirring rod, guarantee to realize between stomach simulated digestive juice and Feed Sample sealing stirring in vertical glass pipe, it is sufficiently mixed, complete the simulation digestion process that stomach stage chyme is wriggled.nullIn this setting time，Digestion reaction is there is in the stomach simulated digestive juice being under stirring and Feed Sample in Dialysis tubing，The digestion product produced is flowed out by the molecular sieve of Dialysis tubing and carries out mass exchange with the stomach buffer in vertical glass tubing，Digestion product dissolves in and can not return in stomach buffer in Dialysis tubing that (digestion product i.e. produced is taken away by stomach buffer，So that produce digestion product do not interfere with Dialysis tubing in carry out subsequently other reaction)，Dialysis tubing has left behind indigested product，Then，The stomach buffer dissolved with digestion product in vertical glass tubing is all discharged，Deionized water is injected in vertical glass tubing via input pipe by pumping peristaltic pump，Vertical glass tubing is flowed out through pumping peristaltic pump by outlet tube after and，Like this，The vertical glass tubing of the deionized water of certain volume inflow and outflow circularly is for several times，So that the digestion product of residual in the stomach buffer remained in vertical glass tubing and Dialysis tubing is cleaned up，Thus complete the simulation digestion process of stomach.
When needing the simulation digestion carrying out small intestine site, above-mentioned stomach simulated digestive juice is replaced by small intestinal simulated digestive juice, stomach buffer exchange is small intestinal buffer, and according to the situation of digesta into duodenum wriggling, set the rotating speed of the stirring rod of small intestinal simulation digestion phase, other operations simulate digestion process with stomach, and then complete the simulation digestion of small intestine site.
When needing the simulation digestion being carried out continuously harmonization of the stomach small intestinal, after the operation completing stomach simulation digestion process, first simulation digestive tube is carried out, vertical glass tubing is regulated again consistent with the ion concentration in Dialysis tubing with small intestinal buffer, build the external environment of small intestinal simulation digestion, then by tube for transfusion, small intestinal simulated digestive juice is injected in Dialysis tubing, and situation about wriggling according to digesta into duodenum, set the rotating speed of the stirring rod of small intestinal simulation digestion phase, proceed small intestinal simulation digestion process operation.
The beneficial effect of a kind of vertical animal gastrointestinal tract Bionic digestion device of the present invention:
1) compared with existing gastrointestinal tract Bionic digestion device, the vertical glass tubing simulation animal gastrointestinal tract digestive environments of sheathed Dialysis tubing in using, due to vertical display when vertical glass tubing uses, there is not liquid in its upper end, the gas that only in simulation digestive tube, residual and digestion reaction produce, vertically in glass tubing, gas is more weak to the active force of silica gel plug, and there is the gravity of effect downwards in silica gel plug, therefore during using, the gas that simulation digestion produces is difficult to come off silica gel plug in the ground of vertical glass tubing end, can be prevented effectively from the glass tubing of traverse in prior art causes the rubber stopper at glass tubing two ends to come off because of digestion reaction aerogenesis in simulation digestion process, the phenomenon that reactant liquor is revealed, improve the Stability and veracity of Bionic digestion；
2) this gastrointestinal tract Bionic digestion device is combined by magnetic drive device and stirring rod, the stirring realized between Feed Sample with simulated digestive juice mixes, reach the simulation that in digestion process, chyme is wriggled, by regulating the mixing speed of stirring rod, complete the simulation digestion process of different parts different phase；
3) pumping-extraction mode that the pumping peristaltic pump connected with input pipe, outlet tube and pumping peristaltic pump are combined is used, and the power of pumping peristaltic pump is less than the power of pumping peristaltic pump, control the simulation digestion buffer flow velocity by vertical glass tubing, and make to be formed between vertical glass tubing and Dialysis tubing relatively low negative pressure, making Dialysis tubing in parting open state a little, the liquid level of the digestion reaction liquid in Dialysis tubing does not fluctuates；Dialysis tubing will not be close to stirring rod because of the liquid stream active force rotating generation of stirring rod, and then, it is to avoid the stirring rod breakage to Dialysis tubing, extend the service life of Dialysis tubing；Simultaneously, it is to avoid treat that sample digestion sticks and cause Dialysis tubing to block at Dialysis tubing tube wall, the error of simulation digestion process is reduced；
4) the first sealing ring of junction or second sealing ring of magnetic drive device and the silica gel plug with tube for transfusion it are fixed on, improve the sealing property of simulation digestive tube, guarantee to simulate the simulated environment temperature in digestive tube consistent, can be avoided digestion reaction liquid and evaporation occurs in simulation digestion process and the phenomenon that causes digestion reaction liquid to reduce, and then improve accuracy and the repeatability that simulation digests；Also allow for maintaining vertical negative pressure between glass tubing and Dialysis tubing in simulation digestion process；
5) simple in construction, easy to use.
Accompanying drawing explanation
Fig. 1 is the perspective view of a kind of vertical animal gastrointestinal tract Bionic digestion device of the present invention；
Fig. 2 is the front view of a kind of vertical animal gastrointestinal tract Bionic digestion device of the present invention；
Fig. 3 is the sectional view of Section A-A in Fig. 2 (vertical animal gastrointestinal tract Bionic digestion device in embodiment 1)；
Fig. 4 is the enlarged diagram in Fig. 3 at C；
Fig. 5 is the sectional view of section B-B in Fig. 2；
Fig. 6 is a kind of vertical animal gastrointestinal tract Bionic digestion device in embodiment 2) front view；
Fig. 7 is the sectional view in D-D cross section in Fig. 6；
Fig. 8 is the enlarged diagram in Fig. 7 at E.
Detailed description of the invention
Below in conjunction with drawings and Examples, the invention will be further described.
Embodiment
1
With reference to Fig. 1 ~ 5, the one vertical animal gastrointestinal tract Bionic digestion device of the present embodiment, including simulation digestive tube, stirring rod 9, motor 8 and magnetic drive device 7；Described simulation digestive tube includes vertical glass tubing 3 and Dialysis tubing 10, and Dialysis tubing 10 is placed in this vertical glass tubing 3；Described stirring rod 9 is arranged in Dialysis tubing 10, and its top removably connects with magnetic drive device 7；Described magnetic drive device 7 is arranged on above simulation digestive tube, electrically connects with motor 8；Described motor 8 is arranged on above magnetic drive device 7 by motor fixing seat 13.
Described vertical glass tubing 3 is a hollow tube, and described hollow tube upper and lower side is respectively provided with a ground 14, and its side bottom and upper end are respectively equipped with input pipe 2 and outlet tube 4；The two ends of Dialysis tubing 10 are stretched out at two grounds 14 of this vertical glass tubing 3 respectively and are turned up, Dialysis tubing 10 end being exposed to outside ground 14 of turning up is tied up by rubber bar and is fixed on ground 14, ground 14 after hollow tube upper and lower side ties up Dialysis tubing 10 end is plugged with the silica gel plug 6 with 3 tube for transfusions 5 and solid silicon plug 1 respectively, offers the groove placing magnetic drive device 7 in the middle part of the described silica gel plug 6 with 3 tube for transfusions 5.
Described input pipe 2 and described outlet tube 4 are centrosymmetric setting relative to the center of described hollow tube.Time actually used, described input pipe 2, described outlet tube 4 lay respectively at lower right, upper left side.
The aperture of described Dialysis tubing 10 is between 700 to 20000 dalton, and described Dialysis tubing 10 is used for containing Feed Sample and simulated digestive juice；The outer tube diameter of described vertical glass tubing 3 is 40 ~ 100mm, the tube length of described vertical glass tubing 3 is 180 ~ 300mm, described vertical glass tubing 3 for being passed through buffer or the deionized water cleaned via described input pipe 2, the feedstuff being easy to heavy dose is simulated digestion process, such as, existing gastrointestinal tract Bionic digestion single treatment amount is generally 2 ~ 3g, and the single treatment amount of the vertical gastrointestinal tract Bionic digestion device after improving is up to more than 20g.
Turn up and be exposed to described Dialysis tubing 10 tip lengths outside described ground 14 between 10 to 25mm.
Described vertical animal gastrointestinal tract Bionic digestion device also includes the first sealing ring (sign) being fixed on magnetic drive device 7 with the junction of the silica gel plug 6 with tube for transfusion 5.
Described magnetic drive device 7 includes that seat 7-2 fixed by magnetic core 7-4, magnetic core；Described magnetic core is fixed and is provided with magnetic loop cover 7-1 outside seat 7-2；Described magnetic core 7-4 fixes seat 7-2 by bearing 7-3 with magnetic core and is connected；The bottom of described magnetic core 7-4 is connected with groove matched in clearance.
Seeing Fig. 4, described stirring rod 9 is connected with magnetic drive device 7 by connecting shaft 11, and the upper end of described connecting shaft 11 is set in outside magnetic core 7-4, its bottom and the hollow top clamping of stirring rod 9.Described connecting shaft 11 is silica gel tube.
Being arranged with motor cover 12 on described motor 8, the bottom of described motor cover 12 is connected with motor fixing seat 13, and motor fixing seat 13 is made up of upper seal bootr 13-1 and lower seal bootr 13-2, and upper seal bootr 13-1 and lower seal bootr 13-2 is bolted to connection.
Embodiment
2
With reference to Fig. 1 ~ 3, Fig. 6 and Fig. 7, the one vertical animal gastrointestinal tract Bionic digestion device of the present embodiment, compared with the vertical animal gastrointestinal tract Bionic digestion device described in embodiment 1, there is following difference:
The quantity of described tube for transfusion 5 is 4.
It is provided with the second sealing ring 21 between described upper seal bootr 13-1 and lower seal bootr 13-2, is bolted to connection.
Seeing Fig. 5, described magnetic drive device 7 includes outer magnetic ring set 17, outer magnetic ring 18, isolation cover 19, internal magnetic ring 20 and internal magnetic ring pitman 22；Described outer magnetic ring set 17 is fixed with motor output shaft, upper seal bootr and is connected；Described outer magnetic ring set 17 is embedded in outer magnetic ring 18；It is provided with isolation cover 19 between described internal magnetic ring 20 and outer magnetic ring 18；Described internal magnetic ring 20 is arranged in isolation cover 19；One end of described internal magnetic ring pitman 22 snaps in internal magnetic ring 20, and its other end is inserted through lower seal bootr 13-2 and silica gel plug 6, is connected with the top of stirring rod 9.
Described vertical animal gastrointestinal tract Bionic digestion device also includes the pumping peristaltic pump 15 being connected with input pipe 2 and the pumping peristaltic pump 16 being connected with outlet tube 4.
Below as a example by the digestion of the harmonization of the stomach small intestinal of simulation animal, further illustrate operation principle and the using method of a kind of vertical animal gastrointestinal tract Bionic digestion device of the present invention.
When the peptic digestion using the present invention to simulate animal, Feed Sample loading Dialysis tubing 10, stomach simulated digestive juice is injected in Dialysis tubing 10 by tube for transfusion 5.Then, there is the stomach buffer of a design temperature, at the input pipe 2 with simulation digestive tube, under the effect pumping peristaltic pump 15 and pumping peristaltic pump 16 that outlet tube 4 connects, inject in vertical glass tubing 3 via input pipe 2, then flow out vertical glass tubing 3 via outlet tube 4, and the power of pumping peristaltic pump 15 is less than the power of pumping peristaltic pump 16, make between the vertical glass tubing 3 of simulation digestive tube and Dialysis tubing 10, to form relatively low negative pressure (such as-0.01MPa), and the liquid level of reactant liquor does not produce fluctuation in bag filter, make that the sample to be digested in bag filter is non-sticky to be adhered on Dialysis tubing 10 tube wall, reduce the error of simulation digestion process.nullLike this，The stomach buffer of certain volume is such vertical glass tubing of inflow and outflow circularly 3 within the setting time，Meanwhile，Motor 8 drives the magnetic core 7-4 of magnetic drive device 7 to fix seat 7-2 relative to magnetic core and does cutting magnetic line movement，And then magnetic core 7-4 rotates，And drive stirring rod 9 to rotate by connecting shaft 11，Or，Motor 8 drives outer magnetic ring set 17 and outer magnetic ring 18 to rotate，Outer magnetic ring 18 is made to produce rotating excitation field in isolation cover 19，Internal magnetic ring 20 is driven to rotate，Snapping in the internal magnetic ring pitman 22 in internal magnetic ring 20 drives stirring rod 9 to rotate，And then realize the stirring of the Feed Sample in bag filter with stomach simulated digestive juice is mixed，Rotating speed >=the 100r/min of stirring rod 9，Guarantee to be sufficiently mixed between stomach simulated digestive juice and Feed Sample，Complete the simulation digestion process that stomach stage chyme is wriggled.nullIn this setting time，Digestion reaction is there is in the stomach simulated digestive juice being under stirring and Feed Sample in Dialysis tubing 10，The digestion product produced is flowed out by the molecular sieve of Dialysis tubing 10 and carries out mass exchange with the stomach buffer in vertical glass tubing 3，Digestion product dissolves in and can not return in stomach buffer in Dialysis tubing 10 that (digestion product i.e. produced is taken away by stomach buffer，So that produce digestion product do not interfere with Dialysis tubing 10 in carry out subsequently other reaction)，Dialysis tubing 10 has left behind indigested product，Then，The stomach buffer dissolved with digestion product in vertical glass tubing 3 is all discharged，Deionized water is injected in vertical glass tubing 3 via input pipe 2 by pumping peristaltic pump 15，Vertical glass tubing 3 is flowed out through pumping peristaltic pump 16 by outlet tube 4 after and，Like this，The vertical glass tubing of the deionized water of certain volume inflow and outflow circularly 3 is for several times，So that the stomach buffer of residual in vertical glass tubing 3 and the digestion product of the interior residual of Dialysis tubing 10 are cleaned up，Thus complete the simulation digestion process of stomach.
When the little intestinal digestion using the present invention to simulate animal, Feed Sample loading Dialysis tubing 10, small intestinal simulated digestive juice is injected in Dialysis tubing 10 by tube for transfusion 5.Then, there is the small intestinal buffer of a design temperature, at the input pipe 2 with simulation digestive tube, under the effect pumping peristaltic pump 15 and pumping peristaltic pump 16 that outlet tube 4 connects, inject in vertical glass tubing 3 via input pipe 2, then flow out vertical glass tubing 3 via outlet tube 4, and the power of pumping peristaltic pump 15 is less than the power of pumping peristaltic pump 16, make between the vertical glass tubing 3 of simulation digestive tube and Dialysis tubing 10, to form relatively low negative pressure (such as-0.01MPa), and the liquid level of reactant liquor does not produce fluctuation in bag filter, make that the sample to be digested in bag filter is non-sticky to be adhered on Dialysis tubing 10 tube wall, reduce the error of simulation digestion process.Like this, the small intestinal buffer of certain volume such vertical glass tubing of inflow and outflow circularly 3 within the setting time；Meanwhile, motor 8 drives the magnetic core 7-4 of magnetic drive device 7 to fix seat 7-2 relative to magnetic core and does cutting magnetic line movement, and then magnetic core 7-4 rotates, and drive stirring rod 9 to rotate by connecting shaft 11, or, or, motor 8 drives outer magnetic ring set 17 and outer magnetic ring 18 to rotate, outer magnetic ring 18 is made to produce rotating excitation field in isolation cover 19, internal magnetic ring 20 is driven to rotate, snapping in the internal magnetic ring pitman 22 in internal magnetic ring 20 drives stirring rod 9 to rotate, and then realize the stirring of the Feed Sample in bag filter with small intestinal simulated digestive juice is mixed, situation about wriggling according to digesta into duodenum, set the rotating speed >=100r/min of stirring rod 9, guarantee to be sufficiently mixed between small intestinal simulated digestive juice and Feed Sample, complete the simulation digestion process that small intestinal stage chyme is wriggled.nullIn this setting time，Digestion reaction is there is in the small intestinal simulated digestive juice being under stirring and Feed Sample in Dialysis tubing 10，The digestion product produced is flowed out by the molecular sieve of Dialysis tubing 10 and carries out mass exchange with the small intestinal buffer in vertical glass tubing 3，Digestion product dissolves in and can not return in small intestinal buffer in Dialysis tubing 10 that (digestion product i.e. produced is taken away by small intestinal buffer，So that produce digestion product do not interfere with Dialysis tubing 10 in carry out subsequently other reaction)，Dialysis tubing 10 has left behind indigested product，Then，The small intestinal buffer dissolved with digestion product in vertical glass tubing 3 is all discharged，Deionized water is injected in vertical glass tubing 3 via input pipe 2 by pumping peristaltic pump 15，Vertical glass tubing 3 is flowed out through pumping peristaltic pump 16 by outlet tube 4 after and，Like this，The vertical glass tubing of the deionized water of certain volume inflow and outflow circularly 3 is for several times，So that the small intestinal buffer of residual in vertical glass tubing 3 and the digestion product of the interior residual of Dialysis tubing 10 are cleaned up，Thus complete the simulation digestion process of small intestine site.
When the little intestinal digestion of harmonization of the stomach using the present invention to simulate animal, the operation of peptic digestion dummy run phase is with the peptic digestion process of above-mentioned simulation animal, therefore repeats no more；After stomach simulation digestion process terminates, then, the stomach buffer dissolved with digestion product in vertical glass tubing 3 is all discharged, deionized water injects in vertical glass tubing 3 by pumping peristaltic pump 15 via input pipe 2, then under the effect of pumping peristaltic pump 16, flow out glass tubing via outlet tube 4, like this, the vertical glass tubing of the deionized water of certain volume inflow and outflow circularly 3 for several times, cleans up with the digestion product by the stomach buffer remained in vertical glass tubing 3 and the interior residual of Dialysis tubing 10.Then, within the second setting time, inject by having the small intestinal buffer of uniform temperature by pumping peristaltic pump 15 input pipe 2 from vertical glass tubing 3 that moves in circles, flow out from the outlet tube 4 of vertical glass tubing 3, so that vertically glass tubing 3 is equal with the ion concentration in Dialysis tubing 10, build small intestinal digestive environments.Then, injecting in Dialysis tubing 10 by small intestinal simulated digestive juice by tube for transfusion 5, subsequent operation is with the simulated operation of above-mentioned small intestine site digestion process.
Claims (9)
1. a vertical animal gastrointestinal tract Bionic digestion device, it is characterised in that include simulating digestive tube, stirring rod, motor and magnetic drive device；Described simulation digestive tube includes vertical glass tubing and Dialysis tubing, and Dialysis tubing is placed in this vertical glass tubing；Described stirring rod is arranged in Dialysis tubing, and its top removably connects with magnetic drive device；Described magnetic drive device is arranged on above the silica gel plug on simulation digestive tube top, electrically connects with motor；Described motor is arranged on above magnetic drive device by motor fixing seat.
Vertical animal gastrointestinal tract Bionic digestion device the most as claimed in claim 1, it is characterised in that described vertical glass tubing is a hollow tube, and described hollow tube upper and lower side is respectively provided with a ground, and its side bottom and upper end are respectively equipped with input pipe and outlet tube；The two ends of Dialysis tubing are stretched out at the two of this vertical glass tubing grounds respectively and are turned up, the Dialysis tubing end being exposed to outside ground of turning up is tied up by rubber bar and is fixed on ground, ground after hollow tube upper and lower side ties up Dialysis tubing end is plugged with the silica gel plug with at least tube for transfusion and solid silicon plug respectively, offers the groove placing magnetic drive device in the middle part of the described silica gel plug with at least tube for transfusion.
Vertical animal gastrointestinal tract Bionic digestion device the most as claimed in claim 2, it is characterised in that described magnetic drive device includes that seat fixed by magnetic core, magnetic core；Described magnetic core is fixed and is provided with magnetic loop cover outside seat；Described magnetic core is fixed seat by bearing with magnetic core and is connected；The bottom of described magnetic core is connected with groove matched in clearance.
Vertical animal gastrointestinal tract Bionic digestion device the most as claimed in claim 3, it is characterised in that described vertical animal gastrointestinal tract Bionic digestion device also includes the first sealing ring being fixed on magnetic drive device with the junction of the silica gel plug with tube for transfusion.
Vertical animal gastrointestinal tract Bionic digestion device the most as claimed in claim 3, it is characterised in that described stirring rod is connected with magnetic drive device by connecting shaft, and the upper end of described connecting shaft is set in outside magnetic core, its bottom and the hollow top clamping of stirring rod.
Vertical animal gastrointestinal tract Bionic digestion device the most as claimed in claim 5, it is characterised in that described connecting shaft is silica gel tube.
Vertical animal gastrointestinal tract Bionic digestion device the most as claimed in claim 1 or 2, it is characterised in that described magnetic drive device includes outer magnetic ring set, outer magnetic ring, isolation cover, internal magnetic ring and internal magnetic ring pitman；Described outer magnetic ring set is fixed with motor output shaft, upper seal bootr and is connected；Described outer magnetic ring is nested and is located in outer magnetic ring；It is provided with isolation cover between described internal magnetic ring and outer magnetic ring；Described internal magnetic ring is arranged in isolation cover；One end of described internal magnetic ring pitman snaps in internal magnetic ring, and its other end is inserted through lower seal bootr and silica gel plug, is connected with the top of stirring rod.
Vertical animal gastrointestinal tract Bionic digestion device the most as claimed in claim 1 or 2, it is characterised in that being arranged with motor cover on described motor, the bottom of described motor cover is connected with motor fixing seat, described motor fixing seat is made up of upper seal bootr and lower seal bootr.
Vertical animal gastrointestinal tract Bionic digestion device the most as claimed in claim 1 or 2, it is characterised in that described vertical animal gastrointestinal tract Bionic digestion device also includes the pumping peristaltic pump being connected with input pipe and the pumping peristaltic pump being connected with outlet tube.
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| CN201610423920.3A CN105974066A (en) | 2016-06-16 | 2016-06-16 | Vertical type animal gastrointestinal tract bionic digestion device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110244006A (en) * | 2019-07-10 | 2019-09-17 | 湖南中本智能科技发展有限公司 | A kind of vertical gastrointestinal tract simulation digest tube of anti-enzyme solution wall built-up |
| CN110244007A (en) * | 2019-07-10 | 2019-09-17 | 湖南中本智能科技发展有限公司 | A kind of Bionic digestion simulator and application method |
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Application publication date: 20160928 |